JOURNAL OF ENVIRONMENTAL MANAGEMENT
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2025
·
IF 8.4
Si-Yi Ren,Zhao-Xing Peng,Jin-Ru Feng,Xi-Ming Zeng,Hong-Gang Ni
Tri-n-butyl phosphate (TBP), a widely used organophosphorus flame retardants, is bioaccumulative, persistent, and toxic. In the real world, heavy metals co-occurrence with TBP often inhibit biodegradation of this pollutant. Immobilized microbial technology offers a promising way for mitigating TBP contamination. In this study, immobilized bacterial microspheres were prepared with sodium alginate (SA) and polyvinyl alcohol (PVA) as carrier materials and Sphingobium xenophagum as the functional microorganism to enhance TBP removal. Magnetic nanoparticles Fe 3 O 4 (sizes of 20 nm, 100 nm, and 200 nm) were introduced to enhance the mechanical strength and microbial activity of the microspheres. The results showed that the optimal environmental conditions for TBP removal were 35 °C and pH 8.0. The Fe 3 O 4 (200 nm)-PVA-SA bacterial microspheres exhibited the best performance, with larger specific surface area and abundant active groups (O-H, -OH, and -COOH). The removal rate exhibited 81.72 % at 5 mg/L within 4 d, which was higher than that of free microorganisms (62.23 %). For Cu 2+ , Zn 2+ , Ni 2+ , and Cd 2+ , the toxicity of Cd 2+ to free microorganisms was greater, while the bacterial microspheres were tolerant to heavy metal stress, especially the Fe 3 O 4 (200 nm)-PVA-SA bacterial microspheres; the TBP removal rate remained at approximately 80.00 % even under multiple heavy metals contamination. Additionally, the Fe 3 O 4 (200 nm)-PVA-SA microspheres maintained high stability and TBP removal rate in 5 consecutive cycles of TBP removal tests and 50 days of storage at 4 °C. The magnetic recyclability, stability, and reusability of the bacterial microspheres offer the potential for efficient and environmentally friendly wastewater treatment. The results provide a theoretical foundation for the development of high-performance immobilized microbial materials and technical support for the effective treatment of organic pollutants in harsh environments.
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CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Wuxia Zhang,Feifei Sun,Hong Niu,Qingjie Wang,Jinyou Duan
As a major component in extracellular matrix of the tissue, chondroitin sulfate A (CS-A) has been shown to exhibit either pro- or anti-inflammatory immune response which was largely dependent on its molecular size and cell types. In this study, we determined the signaling pathway involved in immune response of CS and its N -deacetylated derivative (dCS). Our data indicated that both CS and dCS could activate the NF-κB transcription factor in antigen presenting cells and induce TNF-α production through the TLR/MyD88 pathway. Further studies demonstrated that both CS and dCS had a potential in promoting the proliferation of spleen lymphocytes, and promoting the cytokines secretion by OVA-sensitized splenocytes. Thus, our finding provided a mechanistic insight into the understanding of cellular immunity of CS and dCS, which might be helpful to develop CS-based immune modulators against chronic inflammatory conditions, autoimmunity, infectious diseases, allergies and asthmatic conditions.
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ChemSusChem
·
2019
·Vol. 12
·期 14
·3390-3400
·
IF 7.804
Nan Jia,Jing Liu,Yunshan Gao,Pei Chen,Xinbing Chen,Zhongwei An,Xifei Li,Yu Chen
Graphical Bifunctional electrocatalyst : A nanocomposite with graphene-encapsulated Co 9 S 8 nanoparticles loaded on N,S-codoped carbon nanotubes is fabricated as a highly efficient and stable bifunctional electrocatalyst for the oxygen reduction and evolution reactions. It can be used as the air electrode in the rechargeable Zn–air battery. An inexpensive and efficient bifunctional electrocatalyst for the oxygen reduction reaction (ORR) and the oxygen evolution reaction (OER) is central to the rechargeable zinc–air battery. Herein, a nanohybrid, in which N,S-codoped carbon nanotubes were decorated with Co 9 S 8 nanoparticles encapsulated in porous graphene layers, was fabricated by a one-step heat-treatment process. The N,S dopant species were the major active sites for the ORR, and Co 9 S 8 nanoparticles were mainly responsible for the OER. Compared with commercial 20 wt % Pt/C and Ir/C electrocatalysts, this nanohybrid exhibited a comparable ORR half-wave potential (0.831 V vs. reversible hydrogen electrode) and OER potential (1.591 V at 10 mA cm −2 ), better long-term stability in an alkaline medium, and a narrower potential gap (0.76 V) between ORR and OER. Furthermore, as air electrode of the rechargeable zinc–air battery, it delivered a low charge–discharge voltage gap (0.65 V at 5 mA cm −2 ), high open-circuit potential (1.539 V), good specific capacity (805 mA h g at 5 mA cm −2 ), and excellent cycling stability (48 h), superior to those of commercial Pt/C and Ir/C catalysts, and thus showed promise for applications in renewable energy conversion devices.
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JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Junyu Lu,Jingyi Luan,Yijun Li,Xiwen He,Langxing Chen,Yukui Zhang
Biomedical sciences, and in particular disease biomarker research, demand highly selective and efficient glycoproteins/peptides enrichment platforms. In this work, a facile strategy to prepare hydrophilic maltose-functionalized magnetic metal-organic framework loaded with Au nanoparticles (denoted as [email protected] ) for highly efficient enrichment of N-linked glycopeptides. In brief, carboxyl-functional Fe 3 O 4 nanospheres were firstly coated with a Zr-based MOF shell, the resulting MOF was then loaded with Au nanoparticles in situ and then modified with thiol-functional maltose via Au− S bonds to obtain [email protected] with core-shell structure. The physical property and adsorption of [email protected] to glycopeptides were investigated. The results showed [email protected] possessing the outstanding performance in glycopeptides enrichment with high selectivity (1:200, mass ratio of horseradish peroxidase to bovine serum albumin digest), a low limit of detection (10 fmol), a high recovery (over 83.3%), and a large binding capacity (83 μg•mg −1 ). The [email protected] nanocomposite can enrich 24 and 32 glycopeptides from tryptic HRP and human IgG digests, respectively. Moreover, the nanocomposite was applied to the selective enrichment of glycopeptides from the complex biological samples and a total of 123 unique N-glycosylation sites were identified from 113 glycopeptides in 1 μL of human serum, which were assigned to 46 different glycoproteins. These results showed the promising application of [email protected] in the detection and identification of low-abundance N-linked glycopeptides in complex biological samples.
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Journal of Oleo Science
·
2021
·
IF 1.601
Xiumei Wang,Xiaoxu Zhao,Xiaoli Qin,Zexin Zhao,Bo Yang,Yonghua Wang
n-3 polyunsaturated fatty acids (PUFA)-rich triacylglycerols (TAG) with many beneficial effects are still difficult to be synthesized efficiently and rapidly by current synthetic techniques. This study reports the fatty acid specificity of immobilized MAS1 lipase and its efficient synthesis of n-3 PUFA-rich TAG by esterification of glycerol with n-3 PUFA in natural deep eutectic solvents (NADES) systems. Immobilized MAS1 lipase showed the highest preference for capric acid [C10:0, the highest specificity constant (1/α)=1] whereas it discriminated strongly against docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) due to their lowest specificity constants (1/α=0.19 and 0.2). Moreover, the highest n-3 PUFA-rich TAG content (55.8%) with similar n-3 PUFA composition to the substrate was obtained in choline chloride/glycerol (CG) system. There was a 1.38-fold increase of TAG content in CG system compared with that in the solvent-free system. Interestingly, immobilized MAS1 lipase exhibited no regiospecificity in the solvent-free and various NADES systems. Besides, the potential reaction mechanism of immobilized MAS1 lipase-catalyzed esterification of glycerol with n-3 PUFA in NADES systems was described. It was found that the use of NADES as solvents could greatly enhance TAG content, and make it easy to separate the product. These results indicated that immobilized MAS1 lipase is a promising biocatalyst for the efficient synthesis of n-3 PUFA-rich TAG by esterification of glycerol with n-3 PUFA in NADES systems.
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DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
·
2021
·
IF 3.636
Ling Zhang,Li-na Yao,Wei Liu,An-qi Chen,Shi-min He,Mei-li Wei,Zi-xuan Fan,Da-long Ren
N-acetylcholine receptors (AChRs) are mainly distributed in the postsynaptic membrane and have been widely studied for their control of muscle contraction by regulating neural action potentials. However, the influences of AChRs on immune responses and potential mechanisms remain unclear. Here, we used the advantages of live imaging of zebrafish to explore the regulation process of AChRs on inflammatory responses. Pharmacologically activating of the receptor, we found that the expression of pro-inflammatory cytokines il-1β, il-6, tnf-α and il-8 was significantly up-regulated and neutrophil migration to injury sites was also significantly increased. However, these phenomena were reversed under antagonism of the receptor activity. Results showed that interfering with nAChRs functions did not significantly affect zebrafish motion behavior. Results also showed that activation and antagonism of nAChRs function could regulate the phosphorylation of ERK protein respectively. We further demonstrated that ERK participated in the regulation of AChRs in cytokines expression and neutrophils migration in zebrafish. This study preliminarily revealed the roles of AChRs in inflammatory processes and their potential mechanism, providing additional evidence of peripheral immune regulation by cholinergic receptors.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Liping Sun,Chao Li,Yong Yan,Yue Yu,Hao Zhao,Zijue Zhou,Feng Wang,Yi Feng
Single atom nanozymes (SAzymes) represent the state-of-the-art technology in nanomaterial-based catalysis, which have attracted attentions in catalysis, cancer treatment, disinfection and biosensing fields. However, numerous SAzymes suffered from low aqueous dispersion and without recognition capacity, which impeded their applications in bioanalysis . Herein, we engineered DNA onto SAzymes to obtain the DNA/SAzymes conjugates , which significantly improved the aqueous dispersion and recognition ability of SAzymes. We synthesized iron SAzymes (Fe–N–C SAzymes) as the catalytic nanomaterials, and investigated the interactions between Fe–N–C SAzymes and DNA. We compared A 15 , T 15 and C 15 adsorption of Fe–N–C SAzymes in HEPES containing 2 mM MgCl 2 . We found that 50 μg mL −1 Fe–N–C SAzymes produced nearly 100% A 15 adsorption, 90% T 15 adsorption and only 69% C 15 adsorption, indicating that adenine and thymine had higher adsorption affinity on Fe–N–C SAzymes. More importantly, DNA modification did not affect the peroxidase-like activity of Fe–N–C SAzymes and the bioactivity of the adsorbed DNA. Taking the advantage of the diblock DNA with one DNA sequence (adenine) binding to Fe–N–C SAzymes and the other DNA sequence (i.e., aptamer) binding to cancer cells, we designed Apt/Fe–N–C SAzymes for colorimetric detection of cancer cells, which offered new insights for the use of SAzymes in biomedicine .
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METABOLIC ENGINEERING
·
2017
·
IF 8.142
Lu Dai,Fei Tao,Hongzhi Tang,Yali Guo,Yaling Shen,Ping Xu
Primordial enzymes are proposed to possess broad specificities. Through divergence and evolution, enzymes have been refined to exhibit specificity towards one reaction or substrate, and are thus commonly assumed as “specialists”. However, some enzymes are “generalists” that catalyze a range of substrates and reactions. This property has been defined as enzyme promiscuity and is of great importance for the evolution of new functions. The promiscuities of two enzymes, namely glycerol dehydratase and diol dehydratase, were herein exploited for catalyzing long-chain polyols , including 1,2-butanediol, 1,2,4-butanetriol, erythritol , 1,2-pentanediol, 1,2,5-pentanetriol, and 1,2,6-hexanetriol. The specific activities required for catalyzing these six long-chain polyols were studied via in vitro enzyme assays , and the catalytic efficiencies were increased through protein engineering. The promiscuous functions were subsequently applied in vivo to establish 1,4-butanediol pathways from lignocellulose derived compounds, including xylose and erythritol. In addition, a pathway for 1-pentanol production from 1,2-pentanediol was also constructed. The results suggest that exploiting enzyme promiscuity is promising for exploring new catalysts, which would expand the repertoire of genetic elements available to synthetic biology and may provide a starting point for designing and engineering novel pathways for valuable chemicals.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
·
IF 3.9
Mengxue Li,Juanjuan Wang,Nana Zhu,Yang Zong,Rui Jiao,Zhaoqi Zhu,Hanxue Sun,An Li
Fe–N–C catalysts are some of the most promising catalysts for the oxygen reduction reaction (ORR). However, there are still some problems in the pyrolysis of precursors, such as the destruction of the carbon skeleton and low utilization of active sites. In this work, we synthesized hollow pyridine N-containing conjugated microporous polymers (CMPs) by coupling polymerization. The bipyridine units in CMPs’ skeleton can anchor Fe3+ to form a stable coordination structure, which was effectively converted into Fe–N–C catalysts by one-step pyrolysis. Due to the rigid skeleton of precursors, the hollow structure was preserved in catalysts, which is favorable to O2 diffusion and mass transport of intermediates. X-ray photoelectron spectroscopy and Fe K-edge X-ray near-edge spectroscopy revealed approximate FeN4 structures within the framework of the catalysts. As a result, the resulting CMP-derived catalysts exhibited satisfactory ORR activity in alkaline media with a limiting current density of 4.40 mA cm–2. Furthermore, the Fe,N–C1–900 catalyst demonstrated exceptional methanol immunity with a mere negative shift of 0.01 mA cm–2 in limiting current density compared with the commercial Pt/C catalyst, indicating remarkable stability.
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LUMINESCENCE
·
2025
·Vol. 40
·期 11
·e70357
·
IF 3
Li Li,Yulu Xia,Ming Zhang
Carbon nanoparticles (CNPs) integrated with nanomaterial-based quenchers have emerged as an innovative platform for developing advanced sensing systems. In this study, we developed a novel fluorescent sensing platform for the highly sensitive detection of hydroxylamine (HA). Through a one-step hydrothermal synthesis approach, nitrogen and sulfur co-doped carbon nanoparticles (N,S-CNPs) were successfully prepared using dimethylamine hydrochloride and dimethyl sulfoxide (DMSO) as precursors. The synthesized N,S-CNPs demonstrated exceptional fluorescence properties, achieving a remarkably high quantum yield of 50%, along with outstanding water solubility, photostability, and chemical stability. The fluorescence emission of N,S-CNPs could be effectively quenched by manganese dioxide (MnO 2 ) nanosheets through a combined mechanism of inner filter effect (IFE) and static quenching effect (SQE). Notably, HA induced the redox-mediated decomposition of MnO 2 nanosheets, converting MnO 2 to Mn 2+ ions and consequently restoring the fluorescence of N,S-CNPs (turn-on response). Under optimized conditions, the proposed sensor exhibited a wide linear detection range (0.1–200 μM) with an ultra-low detection limit of 0.04 μM. The sensor demonstrated excellent analytical performance in real-world applications, successfully detecting HA in lake and river water samples with high accuracy and reliability. These findings highlight the great potential of this fluorescent sensing platform for environmental monitoring and water quality assessment.
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Journal of Environmental Chemical Engineering
·
2025
·Vol. 14
·期 1
·120629
·
IF 7.2
Lijun Hu,Qian Huang,Meng Li,Ju Zhang,Qi Xue,Jiayi Wang,Caiwen Li
The parasitic dinoflagellate in Hematodinium genus causes significant economic losses to fishery and aquaculture of multiple valuable marine crustaceans. It is practically needed to develop an effective and environmentally compatible method to control the Hematodinium epidemics in mariculture. In the present study, a novel Z-scheme composite photocatalyst was synthesized successfully using Zinc oxide (ZnO) and Copper Metal-Organic Framework (Cu-MOF), The N-Cu-MOF photocatalyst showed superior charge carrier separation efficiency compared to Cu-MOF, and exhibited significantly improved visible-light absorption in contrast to the activation of ZnO only under UV light. The ZnO/N-Cu-MOF photocatalyst demonstrated excellent inactivation of Hematodinium perezi microspores, with half maximal inhibitory concentration (IC 50 ) values of 3.12 mg/L at 1 h and 1.01 mg/L at 2 h. The inactivation effects involved the generation of large amounts of intracellular reactive oxygen species (ROS), which induced oxidative stress and disrupted the membrane and flagella structural integrity of the dinospores, ultimately leading to cell necrosis induced by oxidative damage. The present study provided a new insight and approach for seawater treatment of mariculture facilities, thereby to prevent and control spreading of Hematodinium epidemics in commercially valuable crustaceans.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Youyou Feng,Jing Qin,Yu Zhou,Qin Yue,Jing Wei
Nanozyme has been regarded as an efficient antibiotic to kill bacteria using the reactive oxygen species (ROS) generated by Fenton-like reaction. However, its activity is still unsatisfied and requires large amount of hydrogen peroxide with side effects toward normal tissues. Herein, spherical mesoporous Fe-N-C single-atom nanozyme (SAzyme) is designed for antibacterial therapy via photothermal treatment enhanced Fenton-like catalysis process. Due to the large pore size (4.0 nm), high specific surface area (413.9 m 2 g −1 ) and uniform diameter (100 nm), the catalytic performance of Fe-N-C SAzyme is greatly improved. The Michaelis-Menten constant ( K m ) is 4.84 mmol L −1 , which is similar with that of horseradish peroxidase (3.7 mmol L −1 ). Moreover, mesoporous Fe-N-C SAzyme shows high photothermal conversion efficiency (23.3 %) owing to the carbon framework. The catalytic activity can be enhanced under light irradiation due to the elevated reaction temperature. The bacteria can also be killed via physical heat effect. Due to the synergistic effect of nanozyme catalysis and photothermal treatment, the antibacterial performance is much higher than that using single antibacterial method. This work provides an alternative for combined antibacterial treatment via photothermal treatment assisted catalytic process using spherical mesoporous single-atom nanozyme as an antibiotic.
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