Discover Oncology
·
2024
·Vol. 15
·期 1
·1-18
·
IF 2.8
Feng Rui,Li Haolin,Meng Tong,Fei Mingtian,Yang Cheng
Purpose This study aimed to construct an m6A and cuproptosis-related long non-coding RNAs (lncRNAs) signature to accurately predict the prognosis of kidney clear cell carcinoma (KIRC) patients using the information acquired from The Cancer Genome Atlas (TCGA) database. Methods First, the co-expression analysis was performed to identify lncRNAs linked with N6-methyladenosine (m6A) and cuproptosis in ccRCC. Then, a model encompassing four candidate lncRNAs was constructed via univariate, least absolute shrinkage together with selection operator (LASSO), and multivariate regression analyses. Furthermore, Kaplan–Meier, principal component, functional enrichment annotation, and nomogram analyses were performed to develop a risk model that could effectively assess medical outcomes for ccRCC cases. Moreover, the cellular function of NFE4 in Caki-1/OS-RC-2 cultures was elucidated through CCK-8/EdU assessments and Transwell experiments. Dataset outcomes indicated that NFE4 can have possible implications in m6A and cuproptosis, and may promote ccRCC progression. Results We constructed a panel of m6A and cuproptosis-related lncRNAs to construct a prognostic prediction model. The Kaplan–Meier and ROC curves showed that the feature had acceptable predictive validity in the TCGA training, test, and complete groups. Furthermore, the m6A and cuproptosis-related lncRNA model indicated higher diagnostic efficiency than other clinical features. Moreover, the NFE4 function analysis indicated a gene associated with m6A and cuproptosis-related lncRNAs in ccRCC. It was also revealed that the proliferation and migration of Caki-1 /OS-RC-2 cells were inhibited in the NFE4 knockdown group. Conclusion Overall, this study indicated that NFE4 and our constructed risk signature could predict outcomes and have potential clinical value.
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PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-18
·
IF 10.6
Wu Cheng,Yan Jing,Ge Chenglong,Xie Lucheng,He Yunjie,Zhao Ziyin,Deng Yekun,Dong Qirong,Yin Lichen
The fracture healing outcome is largely dependent on the quantities as well as osteogenic differentiation capacities of mesenchymal stem cells (MSCs) at the lesion site. Herein, macrophage membrane (MM)-reversibly cloaked nanocomplexes (NCs) are engineered for the lesion-targeted and hierarchical co-delivery of short stromal derived factor-1α peptide (sSDF-1α) and Ckip-1 small interfering RNA (Ckip-1 siRNA, siCkip-1) to promote bone repair by concurrently fostering recruitment and osteogenic differentiation of endogenous MSCs. To construct the NCs, a membrane-penetrating α-helical polypeptide first assembles with siCkip-1, and the cationic NCs are sequentially coated with catalase and an outer shell of sSDF-1α-anchored MM. Due to MM-assisted inflammation homing, intravenously injected NCs could efficiently accumulate at the fractured femur, where catalase decomposes the local hydrogen peroxide to generate oxygen bubbles that drives the shedding of sSDF-1α-anchored MM in the extracellular compartment. The exposed, cationic inner core thus enables robust trans-membrane delivery into MSCs to induce Ckip-1 silencing. Consequently, sSDF-1α-guided MSCs recruitment cooperates with siCkip-1-mediated osteogenic differentiation to facilitate bone formation and accelerate bone fracture healing. This study provides an enlightened strategy for the hierarchical co-delivery of macromolecular drugs into different cellular compartments, and it also renders a promising modality for the management of fracture healing.
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PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 13
·3999-4009
·
IF 4.478
Zhang Liule,Gao Xia,Chen Xiying,Zhao Minyang,Wu Haotian,Liu Yaqing
In view of the high toxicity and widespread availability, it is of great importance to develop accurate, sensitive, and convenient assays for fluoride ion (F − ) detection. Herein, a ratiometric fluorescent system is established for point-of-care testing (POCT) of F − with a smartphone as analyzer. The sensing system of calcein-QDs-Eu 3+ contains two fluorescent probes of calcein (green emission) and ZnCdSe/ZnS QDs (red emission). The sensing system only presents red emission in that the calcein emission is quenched due to the combination between calcein and Eu 3+ . When F − is introduced, the fluorescence of calcein is recovered due to the stronger interaction between F − and Eu 3+ , which changes the emission from red to green. The ratiometric strategy offers an obvious fluorescence color change of the system, which eliminates interference and improves the detection accuracy. Specifically, the sensing system has excellent selectivity in that Eu 3+ is more inclined to bind with F − rather than other anions. The developed assay was further used to prepare a test paper and hydrogel for POCT. To further improve the detection sensitivity and realize quantitative analysis, a smartphone installed with a color scan app is integrated as signal reader and analyzer, which is used for POCT of F − in real samples, showing great application potential in environmental protection and food safety evaluation. Graphical abstract
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高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2021
·Vol. 37
·期 2
·280-285
·
IF 1.307
Chen Yawei,Wang Shiyang,Jie Yulin,Lei Zhanwu,Cao Ruiguo,Jiao Shuhong
Sodium ion batteries(SIBs) are promising energy storage devices for smart grid applications due to their low cost and the high abundance of sodium, but few cathode materials of SIBs with high energy density are available for practical applications. Herein, a series of NaNCM ternary materials(NCM=nickel-cobalt-manganese) is obtained by solid- phase reaction with well-regulated temperature and other reaction conditions. XRD results show that impure NiO phase is more likely to occur under high nickel content. The cross-section SEM indicates that the primary particles in the electrode materials are radially distributed along the radial direction, and the internal porous structure is conducive to the infiltration of electrolyte. The initial specific capacities of Na[Ni 0.68 Co 0.10 Mn 0.22 ]O 2 (NaNCM712), Na[Ni 0.6 Co 0.2 Mn 0.2 ]O 2 (NaNCM622) and Na[Ni 0.4 Co 0.3 Mn 0.3 ]O 2 (NaNCM433) at 0.2 C are 165.5, 153.1 and 146.8 mA·h/g, and the corresponding capacity retention rates are 63.2%, 78.5% and 71.7% after 100 cycles. NaNCM712 possesses the highest initial specific capacity, and NaNCM433 delivers the best rate capability. The rate capabilities of high-nickel and low-cobalt NaNCM cathodes need to be further improved. Moreover, ex-situ XRD pattern reveals the structure evolution (from O3 type to P2 type) during a long cycling charge and discharge process.
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高影响因子
JOURNAL OF FORESTRY RESEARCH
·
2020
·Vol. 32
·期 4
·1779-1787
·
IF 1.689
Cai Hangchuan,Wang Zhiqi,Xie Di,Zhao Panpan,Sun Jianping,Qin Daoyu,Cheng Fangchao
Transparent wood has potential application in intelligent building, solar cell, electronics, and other advanced materials, while its single functionability hinders its further development. Flexible transparent wood (FTW) was prepared by alkaline pretreatment and bleaching treatment of paulownia wood followed by impregnation of epoxy resin and ethylene glycol diglycidyl ether (EDGE). The effect of delignification degree on the optical and mechanical properties of FTW was studied, and the influence of the epoxy/EDGE ratio on the flexibility and mechanical properties of FTW was also investigated. The results showed that higher delignification degree resulted in higher transmittance of FTW. More EDGE addition led to better flexibility of FTW, while overmuch addition of EDGE will reduce the mechanical properties. The optimal FTW sample resulted in a high transmittance of 89% and an ultrahigh haze value of 97% with outstanding flexibility and excellent mechanical properties. The investigation of FTW broadens the research field of transparent wood, and provides great possibility for its application in flexible wearable devices and flexible materials.
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高影响因子
Frontiers of Materials Science
·
2024
·Vol. 18
·期 1
·1-11
·
IF 2.5
Lan Weiwei,Wang Mingbo,Lv Zhenjun,Li Jun,Chen Fuying,Liang Ziwei,Huang Di,Wei Xiaochun,Chen Weiyi
In the field of bone defect repair, critical requirements for favorable cytocompatibility and optimal mechanical properties have propelled research efforts towards the development of composite materials. In this study, carbon nanotubes/polylactic acid/hydroxyapatite (CNTs/PLA/HA) scaffolds with different contents (0.5, 1, 1.5 and 2 wt.%) of CNTs were prepared by the thermally induced phase separation (TIPS) method. The results revealed that the composite scaffolds had uniform pores with high porosities over 68% and high through performances. The addition of CNTs significantly enhanced the mechanical properties of resulted PLA/HA, in which the 1.5 wt.% CNTs/PLA/HA composite scaffold demonstrated the optimum mechanical behaviors with the bending elastic modulus of (868.5 ± 12.34) MPa, the tensile elastic modulus of (209.51 ± 12.73) MPa, and the tensile strength of (3.26 ± 0.61) MPa. Furthermore, L929 cells on the 1.5 wt.% CNTs/PLA/HA scaffold displayed good spreading performance and favorable cytocompatibility. Therefore, it is expected that the 1.5 wt.% CNTs/PLA/HA scaffold has potential applications in bone tissue engineering.
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高影响因子
CHEMISTRY AND TECHNOLOGY OF FUELS AND OILS
·
2024
·Vol. 60
·期 2
·309-314
·
IF 0.6
Liu Tianyu,Ye Zhongbin,Liu Dong
Utilizing emulsion polymerization, a microcapsule breaker with a polystyrene-polyacrylamide core-shell structure was synthesized. Ammonium persulfate served as the breaker, polystyrene as the shell, and polyacrylamide as the drug-carrying agent. The results demonstrated that the synthesized microencapsulated gel-breaker exhibited a uniform spherical shape, superior water dispersion, and enhanced thermal stability. Conductivity tests indicated that the core-shell structure of the microcapsules effectively regulated the release of ammonium persulfate as a gel-breaking agent, resulting in delayed polymer gel-breaking.
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高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2022
·Vol. 33
·期 17
·14167-14177
·
IF 2.779
Zheng YaQian,Hou YaFei,Tan YiNa,Lin Qian,Du Peng,Luo LaiHui,Li WeiPing
Interfacial polarizations might provide important contributions to increase the dielectric constant/polarization of polymer-based nanocomposites, however, with the enhanced interfacial polarization, the insulation (interfacial barriers) and charge–discharge energy efficiency of nanocomposites might be greatly reduced. Therefore, how to balance the coupling effects of interfacial polarizations and interfacial barriers needs to be systematically investigated for optimizing the energy storage performances of nanocomposites. In this study, hierarchical x wt% BT@ST nanofibers with various weight fraction of BaTiO 3 nanoparticles are designed and prepared. Through regulating the weight fraction of BaTiO 3 nanoparticles, the coupling effects of internal interfacial polarizations and interfacial barriers have been explored and optimized, achieving an excellent energy storage performance. For example, the 2 wt% BT@ST/P(VDF-HFP) nanocomposite provides the highest discharged energy density of 17.4 J/cm 3 and charge–discharge energy efficiency of 66% at 453 MV/m due to the simultaneously improved polarization and breakdown strength. The regulation of internal interfacial polarizations and interfacial barriers of hierarchical fillers provide a new approach to design and prepare polymer-based nanocomposites with satisfactory energy storage performances at low filler fraction.
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高影响因子
IRANIAN POLYMER JOURNAL
·
2020
·Vol. 29
·期 3
·275-286
·
IF 1.707
Shao Ming-lu,Yue Xiang-an,He Jie
To better control the gelation time of in situ polymerization of gel system in reservoir, an activator regenerated by AGET (activator generated by electron transfer) ATRP (atom transfer radical polymerization) initiating system was developed. The AGET ATRP initiating system was composed of an initiator 1,2-dibromoethane, a catalyst ferric chloride and a reducing agent vitamin C, and the effects of these three component proportions on the gelation time and gel viscosity were systematically investigated. Results of bottle tests indicated that the gelation time can even be more than 20 h at 60 °C at optimal concentration ratios. Moreover, the viscosity decreased and the gelation time increased with the increase of salinity and temperature. The gelation time of the core test was 1.5 times or even longer in comparison with that of the bottle test owing to the influences of dilution, adsorption, and rock skeleton isolation. It was also confirmed that the AGET ATRP initiating system can initiate the cross-link gelation of monomers in the core. The enhanced oil recovery result indicated that the oil recovery increased by 9.17% after the gelation of the gelling solutions, and final oil recovery was 40.05%. This research provided a brand-new method to control the gelation time of the in situ polymerization of gel system.
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高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2020
·Vol. 39
·期 4
·493-500
·
IF 3.154
Meng Xiao-Yu,Li Yi,Yao Shu-Fang,Wei Xue-Wei,Ye Hai-Mu
Spherulites are the most common crystalline morphology and thus the visual expression of crystal structures for polymers. The diversified patterns have provided intuitive morphology probes for various crystallization behaviors, while the correlations between them are still needed to be enriched. In this work, the complicated spherulitic morphology of poly(propylene fumarate) (PPF), which is sensitive to crystallization temperature, is investigated. PPF melt, respectively, crystallizes into rough spherulites, regularly banded spherulites, and spherulites containing both two kinds of morphology at low, high, and mediate temperatures. By systematically assaying, it is clear that the growth axis along the radial direction changes from a -axis to b -axis as the crystallization temperature increases, which leads to the formation of unique crystallization-temperature-dependent spherulites. Based on detailed characterization of Fourier transform infrared spectroscopy, the packing state of the specific hydrogen bonds of “C=C-H⋯O=C-C=C” in PPF crystal lattices is determined, and furthermore, the mechanism for temperature-dependent selection of growth axes for PPF spherulites in melt is reasonably speculated.
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高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-17
·
IF 14.7
Wu Min,Wang Tingting,Ji Nan,Lu Ting,Yuan Ran,Wu Lingxiang,Zhang Junxia,Li Mengyuan,Cao Penghui,Zhao Jiarui,Li Guanzhang,Li Jianyu,Li Yu,Tang Yujie,Gao Zhengliang,Wang Xiuxing,Cheng Wen,Ge Ming,Cui Gang,Li Rui,Wu Anhua,You Yongping,Zhang Wei,Wang Qianghu,Chen Jian
Glioblastoma (GBM) is the most common brain tumor and remains incurable. Primary GBM cultures are widely used tools for drug screening, but there is a lack of genomic and pharmacological characterization for these primary GBM cultures. Here, we collect 50 patient-derived glioma cell (PDGC) lines and characterize them by whole genome sequencing, RNA sequencing, and drug response screening. We identify three molecular subtypes among PDGCs: mesenchymal (MES), proneural (PN), and oxidative phosphorylation (OXPHOS). Drug response profiling reveals that PN subtype PDGCs are sensitive to tyrosine kinase inhibitors, whereas OXPHOS subtype PDGCs are sensitive to histone deacetylase inhibitors, oxidative phosphorylation inhibitors, and HMG-CoA reductase inhibitors. PN and OXPHOS subtype PDGCs stably form tumors in vivo upon intracranial transplantation into immunodeficient mice, whereas most MES subtype PDGCs fail to form tumors in vivo. In addition, PDGCs cultured by serum-free medium, especially long-passage PDGCs, carry MYC/MYCN amplification, which is rare in GBM patients. Our study provides a valuable resource for understanding primary glioma cell cultures and clinical translation and highlights the problems of serum-free PDGC culture systems that cannot be ignored.
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PubMed
高影响因子
CELLULOSE
·
2020
·Vol. 27
·期 13
·7447-7461
·
IF 4.21
Zhang Caihong,Wu Min,Yang Shuang,Song Xueping,Xu Ying
Abstract The low yield and size nonuniformity of cellulose nanofibrils (CNFs) prepared by mechanical grinding restrict their extensive application in many fields. In this study, microfibrillated cellulose (MFC) with varying grinding time (0–2.5 h) was post-treated with endoglucanase (EG) to improve the yield and size uniformity of the CNFs. With the same grinding time, the yield of the CNFs post-treated with EG increased approximately one-fold, and the diameter of the microfibrils decreased according to the SEM and TEM images. The yield of the CNFs ground for 0.5 h and post-treated by EG was similar to that of the sample ground for 2.5 h, and approximately 26.1 kWh/kg mechanical grinding energy were saved by the EG post-treatment while obtaining the same CNF yield. There was an abundance of micropores that affected the specific surface area of samples, and the width of the micropores was centered between 1.3 and 2.0 nm. The crystallinity index (CrI) of the MFC decreased gradually from 74.90 to 69.30% with prolonged grinding time, whereas the CrI of the samples post-treated by EG slightly increased between 1.7 and 3.0%. The tensile strength and elongation at break of the films prepared by the EG post-treated samples slightly decreased, while the Young’s modulus and transmittance increased. This study provides a valid and an eco-friendly biological method to improve the yield and size uniformity of CNFs and reduce energy consumption. Graphic abstract
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危险品化学品经营许可证（带存储）