CERAMICS INTERNATIONAL
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2021
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IF 4.527
Shiyao Shen,Ying Tang,Jie Li,Weishuang Fang,LaiYuan Ao,Xiangguang Zhao,Liang Fang
Substitution of Ga 3+ by [Zn 0.5 Ti 0.5 ] 3+ in MgGa 2 O 4 improved the comprehensive properties of the resultant spinel. A continuous solid solution with a spinel structure was formed in Mg(Zn 0.5 Ti 0.5 ) 2 x Ga (2−2 x ) O 4 (0 ≤ x ≤ 1); here, Zn 2+ occupied the tetrahedral 8 a site while Ti 4+ occupied the octahedral 16 d site, resulting in a decrease in the degree of inversion λ from 0.86 to 0. The optimal sintering temperature decreased from 1460 °C to 1220 °C, and the dielectric constant ( ε r ) increased from 8.8 to 14.9; these changes could be ascribed to the greater polarizability of [Zn 0.5 Ti 0.5 ] 3+ relative to that of Ga 3+ . The temperature coefficient of resonant frequency ( τ f ) of the material remarkably increased from −64.1 ppm/°C to −16.8 ppm/°C, which may be attributed to the increase in dielectric polarizability/molecular volume, bond energy, and bond valence in B-sites. The high-quality factor ( Q × f ) slightly decreased from 89,253 GHz to 73,432 GHz with decreasing λ and packing fraction. Optimal microwave dielectric properties were obtained from the sample with x = 1 and sample sintered at 1220 °C; in this case, ε r = 14.9, Q × f = 73,432 GHz, and τ f = −16.8 ppm/°C.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
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2016
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IF 5.31
Junyun Huang,Xiaofang Li,Mengjie Ma,Dinglong Li
Di-(2-ethylhexyl) phthalate (DEHP) is an annexing agent that is widely used in the plastics industry. Due to the endocrine-disrupting effect of this compound, the development of an efficient method for degrading DEHP is important. In this study, DEHP was degraded by UV-activated peroxymonosulfate (PMS) oxidation. The influences of various parameters, including PMS dose, temperature, pH, metal ions, common anions and humic acid (HA), were examined. An increase in PMS dose and the presence of metal ions and common anions was observed to accelerate the reaction, while high pH and the presence of HA inhibited the reaction. In addition, seven transformation products were identified by liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS), from which the reaction mechanism and degradation pathways were tentatively proposed. Direct oxidation, hydroxylation, cleavage of the ester bond and decarboxylation were observed in the degradation process. These findings may provide useful information for the treatment of this pollutant.
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FOOD AND CHEMICAL TOXICOLOGY
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2021
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IF 6.025
Xingyao Pei,Haiyang Jiang,Xinyu Liu,Liuan Li,Cun Li,Xilong Xiao,Daowen Li,Shusheng Tang
T-2 toxin, a food-derived mycotoxin , has been identified as a neurotoxin . Nonetheless, T-2 toxin-induced neuroinflammation has never been revealed. As an important therapeutic target for inflammatory diseases and cancers, the role of high mobility group B1 (HMGB1) in mycotoxin-mediated neurotoxicity remains a mystery. In current study, we found that PC12 cells were sensitive to trace amounts of T-2 toxin less than 12 ng/mL, distinguished by decreased cell viability and increased release of lactate dehydrogenase (LDH). Oxidative stress and mitochondrial damage were observed in PC12 cells, manifested as accumulation of oxidative stress products, up-regulation of Nrf2/HO-1 pathway and decrease of mitochondrial membrane potential (MMP), leading to mitochondria-dependent apoptosis . Meanwhile, we first discovered that tiny amounts of T-2 toxin triggered neuroinflammation directly, including raising the expression and translocation of NF-κB and promoting secretion of proinflammatory cytokines such as TNF-α, IL-6, IL-8 and IL-1β. Most interestingly, the increased of HMGB1 was detected both inside and outside the cells. Conversely, HMGB1 siRNA reduced T-2 toxin-mediated oxidative stress, apoptosis and neuroinflammatory outbreak, accompanied by lessened caspase-3 and caspase-9, and decreased secretion of pro-inflammatory cytokines. Taken together, T-2 toxin-stimulated PC12 cells simultaneously displayed apoptosis and inflammation, whereas HMGB1 played a critical role in these neurotoxic processes.
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高影响因子
ACS Applied Materials & Interfaces
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2012
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IF 4.525
Bo Yuan,Qiang Chen,Wen-Quan Ding,Ping-Sheng Liu,Shi-Shan Wu,Si-Cong Lin,Jian Shen,Yue Gai
AB diblock copolymers comprised of poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly(3-methacryloxypropyl trimethoxysilane) (PMTSi) segments, which are used for biocompatible coatings, were investigated. Block copolymers with various compositions were synthesized by atomic transfer radical polymerization (ATRP). The obtained copolymers were dissolved in an ethanol solution, and dynamic light scattering showed that all block copolymers were capable of existing as micelles. After a convenient “one-step” reaction, the cellulose membranes could be covalently modified by these copolymers with stable chemical bonds (C–O–Si and Si–O–Si). Block copolymers with different PMPC chain length were applied to surface modification to find the most suitable copolymer. The functional MPC density can be controlled by adjusting the ratio of the two monomers (MPC and MTSi), which also affect surface properties, including the surface contact angle, surface morphology, and number of functional PC groups. The low-fouling properties were measured by protein adsorption, platelet adhesion and activation, and cell adhesion. Protein adsorption of bovine serum albumin (BSA), fibrinogen, and human plasma were also tested and a moderate monomer composite was attained. The protein adsorption behavior on the novel interfaces depends both on MPC density and PMPC chain length. Platelet adhesion and activation were reduced on all the modified surfaces. The adhesion of Human Embryonic Kidney 293 (293T) cells on the coated surfaces also decreased.
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高影响因子
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
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2017
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IF 2.313
Nan Xiang,Chunfeng Zhao,Xiaoping Diao,Qian Han,Hailong Zhou
Di(2-ethylhexyl) phthalate (DEHP) is recognized as one of the most ubiquitous contaminants in marine environments and causes adverse effects on the health of marine organisms. The purpose of this study was to investigate the toxic effects of DEHP on the pearl oyster Pinctada martensii . The Pinctada martensii was exposed to 0.0, 0.5, 2, 8, or 32 mg L −1 DEHP for 7 and 10 days using parameters of antioxidant. Antioxidant indicators included levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), peroxidase (POD), and total antioxidant capacity (T-AOC) in the gills and hepatopancreas of Pinctada martensii for 7 and 10 days. Besides, we used the lowest observed effect concentration (LOEC) of five enzyme activities in different tissues of Pinctada martensii for 7 and 10 days to compare sensitivity. The results showed that the gills were more sensitive than the hepatopancreas of Pinctada martensii and that GSH activity in the gills and CAT activity in the hepatopancreas might be suitable biomarkers after 7 days of DEHP exposure. After 10 days of DEHP exposure, the GSH activity and CAT activity in the gills and SOD activity in the hepatopancreas could be regarded as biomarkers. Compared to the LOEC, GSH activity in the gills and CAT activity in the hepatopancreas after 7 days of DEHP exposure were more sensitive than any other biomarkers. In addition, after 10 days of DEHP exposure, GSH activity in the gills and hepatopancreas were much more sensitive than other activities. In conclusion, GSH activity demonstrated its potential to be used as a biomarker for the monitoring of DEHP pollution in the marine environment.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2015
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IF 4.152
Zhenzhen Liu,Yong Tang,Ting Kang,Minyu Rao,Kejia Li,Qin Wang,Changyun Quan,Chao Zhang,Qing Jiang,Huiyong Shen
HA and bone morphogenetic protein-2 (BMP-2) possess superior osteoinductive and osteoconductive activities in the repair of bone defects. In this study, a BMP-2 mimicking oligopeptide (serine–serine–valine–proline–threonine, SSVPT) was introduced to the surface of nano-sized HA as the reinforcement phase of a poly(methyl methacrylate) (PMMA) cement, and the synergistic action of HA and oligopeptide on the bioactivity of HA/PMMA bone cement was investigated. Incorporated with 3-trimethoxysilyl propyl methacrylate (MPS)-modified HA, the compressive strength, flexural strength, and flexural modulus of HA/PMMA complied with the international standardization organization 5833 standard. Adherence measurement demonstrated that the introduction of HA and SSVPT could promote the adhesion and proliferation of human fetal osteoblast (hFOB) on the surface of HA/PMMA cement. The increased alkaline phosphatase (ALP) activity indicated that HA/PMMA could promote osteogenic differentiation of hFOB. All of the results illuminated that the HA/PMMA cement could be used as a bioactive material for regeneration and reconstruction of load-bearing bone.
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高影响因子
BIOORGANIC CHEMISTRY
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2020
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IF 4.831
Li Yang,Shengzhang Liu,Ronghua Liu,Junwei He
The root of Dendropanax dentiger (Harms) Merr. is a traditional Chinese medicine that has been used to treat inflammation-related diseases with little scientific validation. In this study, a bioassay-guided phytochemical investigation of D. dentiger led to the isolation of 19 phenylpropanoid derivatives including one new compound ( 1 ) and 18 known ones ( 2 – 19 ). Their structures were elucidated by NMR and HRMS as well as comparison with literature data. The ability of cyclooxygenase-2 (COX-2) inhibition and antioxidant of all isolated compounds were measured in vitro . Chlorogenic acid derivatives ( 14 – 19 ) exhibited outstanding COX-2 inhibitory (IC 50 = 5.1–93.4 μM) and antioxidant (IC 50 = 13.2–31.9 μM) activities. Moreover, the tight structure-activities relationships were proposed. This is the first report on the COX-2 inhibitory activity of phenylpropanoids and D. dentiger .
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高影响因子
Eco-Environment & Health
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2026
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IF 13.4
Kongdong Li,Yichao Zhang,Yingying Zhan,Rongtian Zhang,Ziting Zhao,Kezhuang Chen,Hong Shen,Quanyi Wang,Chang Liu,Wenxiang Zhang,Siyu Chen
DBP-orchestrated liver transcriptional targets are largely associated with HCC. • DBP stabilizes hepatocyte-derived LCN2 to activate cancer cell proliferation. • 24p3R-MMP-9 axis is involved in such a LCN2-triggered cancer cell activation. • Stable DBP-LCN2 complex formation prevents ubiquitin-mediated degradation of LCN2. Dibutyl phthalate (DBP) is a ubiquitous environmental contaminant whose exposure is positively associated with the progression of metabolic dysfunction-associated steatotic liver disease (MASLD). However, the underlying mechanisms by which DBP promotes MASLD progression remain unclear. In this study, high-throughput RNA sequencing revealed that the DBP-orchestrated liver transcriptome is enriched in target clusters associated with hepatocellular carcinoma (HCC), an advanced complication of MASLD. DBP promotes HCC progression in murine models but does not directly affect HCC cell proliferation, indicating that hepatocytes may be the primary target cells for DBP signaling. To identify a molecular target in hepatocytes that transduces DBP signals to HCC cells, we performed label-free proteomic analyses, molecular docking, and the Cancer Genome Atlas (TCGA) database analyses, which identified hepatocyte-derived lipocalin-2 (LCN2) as a candidate target of DBP. Elevated LCN2 expression is negatively associated with overall survival in HCC patients. Inhibition of LCN2 partially attenuates the tumor-promoting effect of DBP-treated hepatocytes on HCC cells. Mechanistically, molecular docking and molecular dynamics simulations demonstrate that DBP directly binds to LCN2 to form a stable complex, which disrupts ubiquitin-proteasome-mediated degradation of LCN2 and thereby enhances its protein stability. Functionally, hepatocyte-derived LCN2 promotes HCC cell proliferation through activation of the 24p3R–matrix metalloproteinase-9 (MMP-9) signaling axis. Our findings demonstrate that DBP acts as a progression promoter in pre-existing HCC via hepatocyte LCN2 stabilization, rather than an initiator of hepatocarcinogenesis. This mechanistic insight addresses a critical gap in understanding how environmental toxicants modulate established HCC progression, complementing epidemiological associations between phthalates and liver disease. Download: Download high-res image (228KB) Download: Download full-size image
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POWDER TECHNOLOGY
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2021
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IF 5.134
Mingyu Zheng,Tiefeng Peng,Yanhong Wang,Liqun Luo,Wei Shao,Jiaqi Gao,Qi Lu
Selective separation of pyrite and galena using 2-mercaptobenzimidazole (MBI) as a chelating collector, was investigated by flotation , scanning electron microscope (SEM), Fourier transform infrared (FT-IR), X-ray photoelectron spectroscopy (XPS) and density functional theory (DFT) calculations. Single-mineral flotation showed a significant flotability of pyrite at pH = 2–4 and galena at pH = 8–10 in the presence of MBI. The depressant effect of pyrite was observed to be more pronounced under alkaline conditions. Artificially mixed minerals flotation suggested the galena-pyrite could be separated under alkaline conditions, especially at pH = 10. SEM analysis indicates that the adsorption of MBI on galena surface is more intense than pyrite. However, the interaction between MBI and pyrite is stronger than that of MBI-galena. FT-IR analysis shows the iron and lead ions may chelate with -N = and -SH groups of MBI. Pyrite contains more iron hydroxide (or sulfate forms) in solution from XPS analysis, only half of the metallic ions on the surface function as active sites, thus reducing the amount of metal that can be chelated with MBI. DFT calculations revealed that MBI was often in the molecular form under acidic mediums and the ionic form under alkaline condition, while the ionic form of MBI was more active.
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高影响因子
ACS Sensors
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2022
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IF 9.618
Haiyang Yu,Huibin Zhang,Jinhua Li,Zheng Zhao,Minhua Deng,Zhanpeng Ren,Ziqin Li,Chenglong Xue,Mitch Guijun Li,Zhaowei Chen
The disease caused by severe acute respiratory syndrome coronavirus, SARS-CoV-2, is termed COVID-19. Even though COVID-19 has been out for more than two years, it is still causing a global pandemic. Due to the limitations of sample collection, transportation, and kit performance, the traditional reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method has a long detection period and high testing costs. An increased risk of infection is inevitable, since many patients may not be diagnosed in time. The CRISPR-Cas13a system can be designed for RNA identification and knockdown, as a promising platform for nucleic acid detection. Here, we designed a solution-gated graphene transistor (SGGT) biosensor based on the CRISPR-Cas13a system. Using the gene-targeting capacity of CRISPR-Cas13a and gate functionalization via multilayer modification, SARS-CoV-2 nucleic acid sequences can be quickly and precisely identified without the need for amplification or fluorescence tagging. The limit of detection (LOD) in both buffer and serum reached the aM level, and the reaction time was about 10 min. The results of the detection of COVID-19 clinical samples from throat swabs agree with RT-PCR. In addition, the interchangeable gates significantly minimize the cost and time of device fabrication. In a nutshell, our biosensor technology is broadly applicable and will be suitable for point-of-care (POC) testing.
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高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2019
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IF 4.561
Zhisheng Zhou,Xing Hu,Xinyue Hong,Jie Zheng,Xia Liu,Deming Gong,Guowen Zhang
5−Hydroxymethyl−2−furaldehyde (5-HMF) is a kind of by-product produced in food processing, which may cause latent hazard to human health. Here, the interaction of 5-HMF with human serum albumin (HSA) was studied by multi-spectroscopic methods, multivariate curve resolution-alternating least squares (MCR−ALS) and molecular docking . The formation of 5-HMF−HSA complex was confirmed by MCR−ALS. The fluorescence quenching mechanism of HSA by 5-HMF was static , and their binding constant was 5.25 × 10 4 L mol −1 at 298 K, the binding site of 5-HMF was located in subdomain IIA of HSA. The main forces of the interaction were hydrogen bonds and van der Waals forces . The molecular docking showed that three hydrogen bonds were formed between –CH 2 OH of 5-HMF and Lys106, Pro147 and Gln29 of HSA. The synchronous fluorescence, three-dimensional fluorescence and circular dichroism spectra showed that 5-HMF induced a structural shrinkage of HSA. Fluorescence phase diagram ascertained that the HSA conformational change was in accordance with all-or-none model. Moreover, the presence of the food nutrients (vitamin C, chlorogenic acid and phloretin) weakened the binding of 5-HMF with HSA. This study is helpful to understand the hazard mechanism of 5-HMF to human body and provides new strategies to reduce the hazard of 5-HMF.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2021
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IF 6.291
Lindong Han,Junke Wang,Tianxin Zhao,Yuhao Wu,Yuexin Wei,Jiadong Chen,Lian Kang,Lianju Shen,Chunlan Long,Zhengwei Yang,Shengde Wu,Guanghui Wei
Di-(2-ethylhexyl) phthalate (DEHP) is the most common phthalate that can affect the male reproductive system. DEHP exposure at the prepubertal stage could lead to the injury of immature testes, but the mechanism has not been fully clarified. In the present study, we elucidated the possible underlying mechanism of DEHP-induced prepubertal testicular injury through stereological analysis and transcriptome profiling. Compared with the control group, the DEHP-treated rats had lower body weight gain and decreased testicular weight and organ coefficient. Moreover, lower serum levels of testosterone and LH were observed in the DEHP group, in contrast to the increased FSH level. Additionally, the serum level of estradiol had no significant difference after DEHP exposure. Stereological analysis showed significant reduction in volumes of most testicular structures, especially in the seminiferous tubule and seminiferous epithelium, along with a vast decrease of spermatogenic cells and obvious structural damages with substantial pathological signs (germ cracks, cytoplasmic vacuolization, sloughing, multinucleated giant cell formation, chromatolysis desquamation and dissolution, pyknosis of nuclei) in the seminiferous tubule upon DEHP exposure at the prepubertal stage. Furthermore, transcriptome profiling identified 5548 differentially expressed genes (DEGs) upon DEHP exposure. Pathway enrichment analysis revealed several crucial signaling pathways related to retinol metabolism, oxidative phosphorylation, steroid hormone biosynthesis, and cell adhesion molecules (CAMs). In addition, seven DEGs selected from RNA-seq data were validated by quantitative real-time polymerase chain reaction (qRT-PCR), and the results showed the same trends as the RNA-seq results. In conclusion, the above findings provide basic morphological data and lay a foundation for systematic research on transcriptome profiling in prepubertal testicular injury induced by DEHP.
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高影响因子
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