APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2025
·
IF 3.3
Zhang Xiuju,Nie Xiaowei,Yu Wenxiao,Du Guanchao,Liu Shaoming,Song Yewen
MicroRNA (miRNA) has emerged as a potential diagnostic marker for malignancies, such as prostate cancer; yet, few analytical approaches have been established for the simple and effective identification and quantification of miRNA. We depict here a fluorescent dumbbell probe utilizing DNA-silver nanoclusters (DNA-AgNCs) to facilitate the simple and label-free quantification of miRNA. The fluorescent probe was methodically engineered using a double-flapped dumbbell structure, wherein the 5′ flap was synthesized using DNA-AgNCs, and the 3′ flap was extended by a G-rich sequence. The DNA scaffold rigidifies the DNA-AgNCs and the G-rich sequence, bringing them into close contact, which leads to enhanced fluorescence due to the activation of DNA-AgNCs by the G-rich sequence. Upon the introduction of miRNA, the 5′ flap of the probe unfolds, liberating the G-rich sequence from the probe, disrupting the closeness between DNA-AgNCs and the G-rich region, and resulting in decreased fluorescence. The proposed method, by incorporating target recycling and polymerase/endonuclease-assisted cycles, enables sensitive detection of miRNA with a detection limit of 4.2 fM and demonstrates high specificity for monitoring target miRNA in clinical samples, offering a robust platform for miRNA monitoring and disease diagnosis. Graphical
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FOOD SCIENCE AND BIOTECHNOLOGY
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2025
·
IF 3.1
Li Hang,Li Mengyuan,Zhang Manman,Liu Ruixue,Li Nan,Wang Yongsheng,Wang Zhihan,Wang Shihan
This study demonstrated the commercialization potential of Rana chensinensis ovum protein isolates (RCOPI) by comparing it with commercially available proteins from physicochemical, structural, and functional properties to stabilizing Pickering emulsions. The size of RCOPI colloids nanoparticles reached 65 nm which is much smaller than the commercially available proteins. Besides, RCOPI exhibited incredible emulsion ability with the emulsion drop around 22 μm diameters and the zeta potential approximately − 50 mV. The emulsions stabilized by RCOPI showed remarkable stability and the Lactalysis index was about 21% after 100 days storage. In the encapsulation test, scanning electron microscopy (SEM) showed the size of the microcapsule ranging from 20 to 50 μm. The encapsulated efficiency of RCOPI stabilized emulsion was as good as the compared proteins, up to 75%. Furthermore, the results of the sensory evaluation showed that RCOPI stabilized emulsions received the highest approval ratings for appearance, taste, flavor and overall acceptability. Graphic
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Science China-Materials
·
2025
·
IF 7.4
Li Junlong,Su Hao,Huang Wei,Chen Rong,Le Jianbi,Lin Min,Zhou Xiongtu,Zhang Yongai,Guo Tailiang,Wu Chaoxing
Alternating current (AC) driven quantum dots (QDs) light-emitting diodes have attracted widespread attention due to their unique optoelectronic performance. An AC-driven device with an interdigital electrode structure is proposed. The devices exhibit AC electroluminescence (EL) with driving frequency and different frequency responses due to the introduction of P(VDF-TrFE-CFE). The optical and electrical characteristics of the device under various applied voltages and driving frequencies are studied. Unlike the conventional driving mode where carriers are continuously injected into the device, the carriers undergo periodic motion under an AC field. Therefore, there exists an optimal driving frequency that maximizes the EL intensity of the device. Based on the experimental results, the operational mechanism of the device is analyzed, and a carrier transport model is proposed to elucidate the underlying factors contributing to the presence of an optimal driving frequency. Moreover, a model for the device is proposed and its accuracy is verified. From a circuit perspective, the EL behavior of devices at various driving frequencies is analyzed, thereby further confirming the accuracy of the proposed device working mechanism. We believe the work can provide guidance for obtaining advanced QD-based light-emitting technology.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-10
·
IF 15.7
Li Weipeng,Xu Weizhuo,Sun Zhaopeng,Tang Linning,Xu Guohao,He Xinyue,Deng Yulin,Sun Wei,Zhou Bingjie,Song Jianfei,Liu Wei
As a promising stationary energy storage device, aqueous redox flow battery (ARFB) still faces the challenge of low open-circuit voltage, due to the limitation of the potential of water splitting (1.23 V theoretically). Herein, we present a low potential anolyte design by using Na substituted phosphotungstic acid (3Na-PW 12 ) for an aqueous redox flow battery with the high open-circuit voltage up to 2.0 V. The 3Na-PW 12 can store 5 electrons in the charging process and simultaneously capture Na + or protons from the dissociation of water, resulting in the increase of electrolyte pH to 11. Because of the high pH value, the hydrogen evolution reaction (HER) is highly suppressed, and the 3Na-PW 12 is partially degraded into a lacunary structured PW 11 with extremely low potential down to −1.1 V (vs. SHE). After discharging, the captured protons are re-released into the solution, therefore, pH and the structure of 3Na-PW 12 are recovered. Based on the cyclic pH change and self-regulation process of 3Na-PW 12 in the charge and discharge process, the aqueous flow battery offered a high-power density of 200 mW cm −2 and 160 mW cm −2 coupled with Br 2 /Br − and I 2 /I − catholyte respectively.
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Science China-Technological Sciences
·
2025
·Vol. 68
·期 6
·1-10
·
IF 4.9
Zeng Xu,Liu Xintian,Sun Zhengnan,Wang Yilin,He Changliu,Tang Wenjie,Zhang Xiaosheng
In the modern age of the Internet of Things, nanogenerators have advanced due to their stability, portability, and sustainability. This study focuses on high-performance wood-based nanogenerators. Natural wood possesses a unique three-dimensional microstructure with hierarchical, interconnected channels aligned along its growth direction. This porous, highly oriented structure of wood was fully studied and maintained during the whole delignification process. Calcium chloride and silver nanowires were used as backfilling agents in the delignified wood, enhancing its electrical conductivity. The resulting conductive wood served as both the friction layer and electrode, forming a single-electrode triboelectric nanogenerator with excellent electrical output performance. Our findings highlight the potential of multifunctional wood as an eco-friendly, sustainable energy source, contributing to the future of smart cities.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-13
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IF 3.9
Meng Zhuo-Nan,Chen Jian-Yuan,Yu Chong,Zheng Ai-hong,Reddy O. Sreekanth,Liu Kai-Yan,Su Yong-Rui,Zhang Shi-Tai,Wang Yin-Shuang,Gu Hang-Yu,Wang Fu-Wei,Xu Song-Cheng,Sun Li-Tao,Chen Bing-Chen,Lai Wing-Fu,Wu Guo-Qing,Zhang Da-Hong
Surgical resection of the tumor remains the preferred treatment for most solid tumors at an early stage, however, residual tumor cells after surgical resection poses a considerable obstacle in cancer treatment. Here, we developed a gel carrier using a cellulose-based gel-forming polymer (CT) combined with gelatin sponge (GS) to fill the resection cavity and delay postoperative residual tumor growth. The fabricated gel exhibited a porous nature along with gradual swelling and erosion over time. Curcumin (Cur) and apatinib (Apa) were loaded into CT gel (CT-CA), and a sustained release behavior was observed at pH 7.4 and 6.4 at 37 °C. The preclinical studies indicated that the mouse weight and tissue exhibited no apparent change after administration of the GS-CT compared with the control. The in vivo fluorescence images showed that GS-CT has the capability to regulate the release of Cur and Apa, facilitating the accumulation of these two agents at the surgical tumor site. Moreover, GS-CT loaded Cur and Apa (GS-CT-CA) delayed postoperative residual tumor growth in intraperitoneal and subcutaneous postoperative mouse models. These findings demonstrated that our gel carrier system significantly prevents postoperative residual tumor growth because of enhanced drug accumulation and sustained drug release at the tumor site.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-8
·
IF 15.7
Sun Wenlu,He Yulu,Fang Lei,Zeng Jinrong,Tang Enli,Hu Tingping,Zhang Hui,Lu Derong,Hu Chenji,Quan Zhilong,Kong Huabin,Chen Hongwei
Enhancing the durability of electrodes under high-power conditions is crucial for advancing high-power secondary batteries. Despite numerous optimization strategies proposed to mitigate electrode structural degradation, the performance of common electrodes inevitably deteriorates under high currents over extended cycling life. In this study, we report a capacity refreshing strategy for a porous organic framework electrode operating under high power conditions. By intermittently applying low currents, deactivated ions trapped within the framework can be effectively released, thus refreshing the electrode’s capacity. Importantly, this capacity refreshing can be periodically repeated. The framework electrode thus achieves an extended cycle life of over 60,000 cycles at 20 C (6 A/g) with a specific power of 28 kW/kg (based on active material). This strategy represents an alternative concept for designing organic electrodes that combine high power and long cycle life.
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CATALYSIS LETTERS
·
2018
·Vol. 148
·期 6
·1714-1722
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IF 2.911
Luo Wei,Deng Xin-Xing,Huo Jiao,Ruan Tao,Gong Zhi-Wei,Yan Jia-Bao,Yang Zhong-Hua,Quan Can,Cui Zhi-Fang
Abstract Microalgae are excellent biocatalyst candidates for photo-biocatalytic-asymmetric reduction of prochiral ketones to produce enantiomer alcohols. In the biocatalysis asymmetric reduction of carbonyl group process, the cofactor, NADPH, plays a key role. So the current study focused on the effect of NADPH availability on the reduction. Photosynthesis is a main pathway for NADPH regeneration in microalgae, and the ferredoxin-NADP + oxidoreductase (FNR) is the key enzyme in this process. In this work, we constructed an engineered cyanobacterium Synechocystis sp. PCC6803:: Ω- P petE-petH to overexpress FNR gene petH to strengthen NADPH regeneration. The results show that the intracellular NADPH content was increased 80.8% compared to the wild type. Applying the Synechocystis sp. PCC6803:: Ω- P petE-petH to catalyze the model asymmetric reduction reaction (reduction of acetophenone to S -phenylethanol), the yield was improved 61.2% compared to the wild-type. This work makes the microalgal biocatalysis asymmetric reduction process become more preponderant via providing a new route to enhance the regeneration of cofactor NADPH. Graphical Abstract Putative metabolic process of nicotinamide adenine dinucleotide phosphate (NADPH) regeneration and the photo-biocatalytic-asymmetric reduction reaction of prochiral ketones in microalgal cells.
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Cell Death & Disease
·
2023
·Vol. 14
·期 10
·1-17
·
IF 9
Deng Huan,Chen Yamei,Wang Li,Zhang Yibi,Hang Qingqing,Li Peijing,Zhang Peng,Ji Jing,Song Hai,Chen Ming,Jin Ying
Our previous study revealed that PI3K/AKT/mTOR signaling was associated with SCLC radioresistance. SBC2 cells were used as primary radioresistance models, while H446 cells were continuously exposed to ionizing radiation (IR) to develop acquired radioresistance. Cell viability and apoptosis assays were used to investigate synergistic effects of BEZ235/GSK2126458 and IR in vitro, while immunoblotting, metabolite quantitative analysis and bioinformatic analyses were utilized to explore the underlying mechanism. Both genetically engineered mouse models (GEMM) and subcutaneous tumor models were used to confirm the synergistic effect in vivo. Key molecules of PI3K/AKT/mTOR signaling were upregulated after IR, which was correlated with primary radioresistance, and they were more expressed in acquired radioresistant cells. BEZ235/GSK2126458 effectively enhanced the cytotoxic effects of IR. BEZ235/GSK2126458 plus IR elevated γ-H2AX and p-Nrf2 expression, suggesting DNA and oxidative stress damage were intensified. Mechanistically, BEZ235/GSK2126458 plus IR significantly reduced the expression of G6PD protein, the rate-limiting enzyme of the pentose phosphate pathway (PPP). In detail, PI3K/mTOR inhibitors reinforced interaction between G6PD and HSPA8/HSC70, and G6PD was degraded by chaperone-mediated autophagy processes. Their metabolites (NADPH and R-5P) were decreased, and ROS levels were indirectly elevated, both of which exacerbated cell death. PI3K/AKT/mTOR signaling activator, insulin, enhanced SCLC radioresistance, while the synergistic effect of BEZ235/GSK2126458 and IR can be attenuated by N-acetylcysteine, and enhanced by 6-amino niacinamide. GEMM and allograft transplantation assays further confirmed their synergistic effect in vivo. This study provided insights into the connection between PI3K/AKT/mTOR signaling and the PPP underlying radioresistance and provided evidence of mechanisms supporting PI3K/mTOR inhibitors as possible therapeutic strategies to abrogate SCLC radioresistance.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2021
·Vol. 19
·期 1
·1-16
·
IF 10.435
Zhao Jiayan,Wu Hang,Zhao Jiulong,Yin Yichen,Zhang Zhilun,Wang Shige,Lin Kun
Owing to the hypoxia status of the tumor, the reactive oxygen species (ROS) production during photodynamic therapy (PDT) of the tumor is less efficient. Herein, a facile method which involves the synthesis of Mg–Mn–Al layered double hydroxides (LDH) clay with MoS2 doping in the surface and anionic layer space of LDH was presented, to integrate the photo-thermal effect of MoS2 and imaging and catalytic functions of Mg–Mn–Al LDH. The designed LDH-MoS2 (LMM) clay composite was further surface-coated with bovine serum albumin (BSA) to maintain the colloidal stability of LMM in physiological environment. A photosensitizer, chlorin e6 (Ce6), was absorbed at the surface and anionic layer space of LMM@BSA. In the LMM formulation, the magnetic resonance imaging of Mg–Mn–Al LDH was enhanced thanks to the reduced and acid microenvironment of the tumor. Notably, the ROS production and PDT efficiency of Ce6 were significantly improved, because LMM@BSA could catalyze the decomposing of the overexpressed H2O2 in tumors to produce oxygen. The biocompatible LMM@BSA that played the synergism with tumor microenvironment is a promising candidate for the effective treatment of cancer.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 19
·4629-4638
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IF 3.637
Munyemana Jean Claude,Chen Jia,Wei Xin,Ali Mohammad Chand,Han Yangxia,Qiu Hongdeng
In the quest for alternative products that would conquer natural enzyme drawbacks, enzyme-like nanomaterials with controllable morphology, high catalytic activity, excellent stability, and reusability have gained extensive attention in recent years. Herein, a simple and versatile strategy based on basic deep eutectic solvents was used to create layered copper hydroxide nitrate (Cu 2 (OH) 3 NO 3 ) with a well-structured nanosheet-like morphology. The present nanosheets exhibited extraordinary oxidase and peroxidase-like activity. More importantly, these nanosheets have shown the ability to operate at low and high temperatures with appreciable stability and multiple reusabilities. Based on inhibiting the oxidase-like activity of the prepared Cu 2 (OH) 3 NO 3 , we designed a colorimetric sensing technique with a high-efficiency detection of biothiols in serum samples. Because of the simplicity and low-cost fabrication approach, our findings would be beneficial to the artificial enzyme research community as another facile and green tactics to fabricate heterogeneous artificial enzymes.
Graphical abstract
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MONATSHEFTE FUR CHEMIE
·
2015
·Vol. 146
·期 8
·1343-1347
·
IF 1.222
Qiu Dezhi,Wang Yanyan,Lu Dongming,Zhou Lihong,Zeng Qingle
Abstract We have developed a KOH-promoted transition-metal-free synthesis of 2-substituted 4(3 H )-quinazolinones from anthranilamides and benzyl alcohols or cinnamyl alcohol with air as oxidant. The protocol is simple, practical, and cost saving. Graphical abstract
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危险品化学品经营许可证（带存储）