ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Jianguo Hou,Binyun Xia,Kunpeng Niu,Jiale Wang,Jinhua Li,Ning Wang
Background Cobalt, an essential trace element, is vital for maintaining human nervous system function, aiding in DNA synthesis, and contributing to red blood cell production. It is helpful for disease diagnosis and treatment plan evaluation by precisely monitoring its concentration changes in the human body. Despite extensive efforts made, due to its ultra-low concentration, the current limit of detection (LOD) as reported is still inadequate and cannot be satisfied with the precise clinical applications. Therefore, it is crucial to develop novel label-free sensors with high sensitivity and excellent selectivity for detecting trace amounts of Co 2+ . Results Here, an ultrasensitive optical fiber SPR sensor was designed and fabricated for label-free detection of Co 2+ with ultra-low concentration. It is achieved by modifying the carboxyl-functionalized CQDs on the AuNPs/Au film-coated hetero-core fiber, which can specifically capture the Co 2+ , leading to changes in the fiber's surface refractive index (RI) and subsequent SPR wavelength shifts in the transmission spectrum. Both the Au film and AuNPs on the fiber are modified with CQDs, leveraging their large surface area to enhance the number of active sites and probes. The sensor exhibits an ultra-high sensitivity of approximately 6.67 × 10 19 nm/M, and the LOD is obtained as low as 5.36 × 10 −20 M which is several orders of magnitude lower compared to other conventional methods. It is also experimentally demonstrated that the sensor possesses excellent specificity, stability, and repeatability, which may be adapted for detecting real clinical samples. Significance The CQDs-functionalized optical fiber SPR sensor exhibits substantial potential for precisely detecting Co 2+ of trace amounts, which is especially vital for scarce clinical samples. Additionally, the sensing platform with sample sensor fabrication and measurement configuration introduces a novel, highly sensitive approach to biochemical analysis, particularly adapting for applications involving the detection of trace targets, which could also be employed to detect various biochemical targets by facile modification of CQDs with specific groups or biomolecules.
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PubMed
高影响因子
Small
·
2025
·
IF 12.1
Chenggong Zhu,Qunfang Huang,Ruijie Fu,Zhen Xun,Qishui Ou,Yunlei Xianyu,Can Liu
Hepatitis B virus (HBV) infection remains a significant global public health issue, and rapid detection of HBV DNA is crucial for disease prevention and control. However, traditional methods for HBV DNA detection are limited by their reliance on precise instruments and single readout, which can hardly meet the requirements of on-site detection. In this study, the Mg 2+ -enhanced trans-cleavage activity of clustered regularly interspaced short palindromic repeats/associated protein 12a (CRISPR/Cas12a) is reported and coupled with loop-mediated isothermal amplification (LAMP) and Au@Pt nanoparticles as a signaling reporter for on-site detection of HBV DNA. This triple-modal biosensing strategy enables multiple signal readouts including UV–vis spectrum, RGB value, and temperature with high sensitivity and accuracy. The linear detection ranges using UV–vis spectroscopy, RGB color recognition, and photothermal modes are from 50 to 10,000 copies µL −1 , with the limitation of detection of 24.07, 39.65, and 23.33 copies µL −1 , respectively. This biosensing strategy is further employed for the qualitative detection of HBV DNA in 48 serum samples, achieving sensitivities of 100%, 100%, and 95.24% for triple modes. This work offers a promising tool of next-generation LAMP-CRISPR/Cas12a for the rapid and portable detection of nucleic acids.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Shuo Tian,Jiaping Lin,Wei Chen,Dianping Tang,Yang Ou
The development of rapid and sensitive CA19–9 (carbohydrate antigen 19–9) analytical methods for early screening of potential patients, especially in clinical situations, is important for controlling the progression of pancreatic cancer. Herein, a new photoelectrochemical (PEC) immunoassay has been devised for the ultrasensitive detection of CA19–9 using gold nanoparticle-modified ultrathin ZnIn 2 S 4 nanosheets (Au-UZNS) as the photoactive substrate. Notably, the alkaline phosphatase (ALP) labeled on the detection antibody (ALP-dAb) serves as the signal amplifier that catalyzes the production of large amounts of ascorbic acid (AA) after the sandwiched immunoreaction. The generated AA is an outstanding electron donor, thereby improving the intensity of the photoactive substrate. Under the optimized parameters, the Au-UZNS-based PEC immunosensor displays an excellent response to CA19–9 ranging from 0.01 to 500 U mL −1 with a limit of detection (LOD) of 4.7 mU mL −1 . Generally, the CA19–9 levels in the serum of high-risk pancreatic cancer patients range from 120 to 400 U mL −1 , while the levels in normal humans range from 0 to 40 U mL −1 . Therefore, the exceptionally low LOD value of the proposed method is sufficient to meet the threshold for clinical detection and holds significant potential for further application.
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高影响因子
TALANTA
·
2025
·
IF 6.1
Qiujin Li,Jianing Ren,Kexin Feng,Jixian Gong,Zheng Li,Xiuming Liu,Jianfei Zhang
Wearable glucose sensors have received widespread attention for their ability to provide real-time and continuous physiological information through non-invasive detection of glucose in sweat, which is significant to wellness assessment and management. However, there are still challenges of glucose catalysis and sweat sampling in developing efficient wearable glucose sensors. Here, we report a flexible wearable electrochemical sensor based on a Janus nanofiber membrane for efficient sweat collection and glucose detection. Leveraging the high reducing capability of MXene, gold nanoparticles (AuNPs) were synthesized in situ, and then functionalize the screen-printed carbon electrode (SPCE) substrate. The synergistic effect derived from the high specific surface area and superior redox activity of MXene-Au composites, as well as the efficient immobilization of glucose oxidase (GOx) make the sensor with high sensitivity and accuracy. A polyurethane/polyvinyl alcohol (PU/PVA) Janus fiber membrane, fabricated via electrospinning, served as a rapid unidirectional water transport component for active sweat collection. This electrochemical sensor exhibits a wide linear range encompassing the typical physiological glucose concentration profile in sweat, with a sensitivity of 0.33 μA μM −1 cm −2 and a detection limit of 13.0 μM. The sensor demonstrates excellent selectivity for glucose with good reproducibility and stability, suggesting significant promise for providing a new strategy towards non-invasive glucose monitoring.
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Nanoscale
·
2025
·
IF 5.1
Mi Li,Haorong Cheng,Yang Song,Xinxin Shi,Tongxiang Li,Yan Chen,Zhao Li,Lin Tian
The rapid detection of sulfide compounds including glutathione (GSH) and Na2S2O8 is of great interest because they play an essential role in the process of food processing. Here, we design a colorimetric/smartphone dual-mode sensing platform to detect GSH and Na2S2O8 simultaneously, depending on gold nanoparticle-decorated MnO2 nanosheets (Au/MnO2 NSs). Because of the distinct structure, Au/MnO2 possesses superior catalytic activity similar to native oxidase capable of catalysing the directly oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) under near-neutral pH conditions. Meanwhile, this catalytic reaction results in an obvious color change or the solution, which senses GSH and Na2S2O8 through redox and diazotization reactions. The designed sensor enables value-assisted and visual determination of GSH and Na2S2O8 with limits of detection of 2.3 nM. Based on the dual-modal sensing system, a smartphone-mediated “all-in-one” sensing platform is designed to realize the real-time and intelligent monitoring of GSH and Na2S2O8. This work provides a new avenue to construct multifunctional sensors to achieve the visualization and portable determination of sulfide compounds.
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Nano-Micro Letters
·
2023
·Vol. 15
·期 1
·1-13
·
IF 26.6
Gao Manyi,Tian Fenyang,Zhang Xin,Chen Zhaoyu,Yang Weiwei,Yu Yongsheng
A hybrid co-catalysts system comprising Au nanoparticles (NPs) and PtSAs with different functions was constructed on the polymeric carbon nitride (PCN) surface by double-solvent method.
For the dual co-catalysts, the Au NPs absorb relatively long-wavelength light to produce plasmonic hot-electrons, and the adjacent Pt single atoms (PtSAs) can trap the plasmonic hot-electrons effectively for H 2 evolution.
The PtSAs–Au 2.5 /PCN exhibits excellent broad-spectrum photocatalytic H 2 evolution activity with the H 2 evolution rate of 8.8 mmol g − 1 h − 1 at 420 nm and 264 μmol g − 1 h − 1 at 550 nm, respectively.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2024
·
IF 20.2
Tianwei Dou,Yangyang Zhu,Zhanyu Chu,Lei Sun,Zhijun Li,Liqiang Jing
TiO 2 mesoporous hollow nanofibers with excellent mass diffusion is an attractive photocatalytic material to remove toxic gaseous pollutants like CO, while it is highly desired to improve the photogenerated charge separation and O 2 activation. Herein, the Au nanoparticles inner-loaded TiO 2 mesoporous hollow nanofibers (Au@TO MPHNFs) have been controllably fabricated by one-step coaxial electrospinning, in which it is crucial to use polyethyleneimine as porogen and stabilizer for mesoporous and TiO 2 in nanofibers shell. Concurrently, a stepwise phase transfer technique is employed to realize the uniform and stable dispersion of the Au in the paraffin oil as core precursor. The Au@TO MPHNFs exhibits 2.7 and 7.4-fold CO photooxidation enhancement compared with commercial P25 and TO MPHNFs, respectively. The enhanced photoactivity can be attributed to the creation of the mesoporous hollow structure for facilitating better mass transportation, and the incorporation of Au nanoparticles for efficient charge separation and O 2 activation. Meanwhile, the photothermal effect of Au expedites intermediaries transformation and product desorption.
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高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Shuai Zhao,Haojia Qin,Jie Huang,Qiang Chen,Shangzhong Jin,Pei Liang
Rapid determination of pesticide residues in water is of vital importance for human health. However, rapid, reliable, and highly sensitive detection of pesticide residues in water environments remains a challenging issue. In this paper, we prepare Fe 3 O 4 /MOF-867 composites using the hot solvent method, and Au + is reduced to Au NPs by the reducing agent and attached to the film of Fe 3 O 4 /MOF-867 composites by the addition of APTES as a linker to synthesize the Au/Fe 3 O 4 /MOF-867 SERS substrates. Then, we change the Fe 3 O 4 content and the concentration of the reducing agent to obtain the optimal substrate. The substrate has a detection capability of up to 10 −9 M for probe molecule R6G and the RSD is less than 5%, which demonstrates that the substrate has excellent SERS effect and reproducibility. In addition, the Au/Fe 3 O 4 /MOF-867 SERS substrates have been applied for the determination of thiram in lake water samples, with its limit of detection (LOD) up to 3.8 × 10 −10 M. The Au/Fe 3 O 4 /MOF-867 SERS substrates can quickly and efficiently collect target analytes by magnetic enrichment, have high SERS performance, and have great potential for the rapid and sensitive detection of pesticide residues and other pollutants in the environment.
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SENSORS
·
2024
·Vol. 24
·期 23
·7636
·
IF 3.4
Yan Yu,Haiyu Xie,Tao Zhou,Haonan Zhang,Chenze Lu,Ran Tao,Zhaozhao Tang,Jingting Luo
Prostate-specific antigen (PSA) is a well-established tumour marker for prostatic carcinoma. In this study, we present a novel, real-time, and ultrasensitive Love-mode surface acoustic wave (L-SAW) immunosensor for PSA detection enhanced by MoS2@Cu2O-Au nanocomposite conjugation. The MoS2@Cu2O-Au nanocomposites were analyzed by SEM, XRD, and EDS. The experiments show a significant improvement in sensitivity and detection limit compared with the previous detection methods utilizing nanogold alone to detect PSA biomolecules. The experimental results show a good linear relationship when the range of PSA concentrations between 200 pg/mL and 5 ng/mL was tested. The experimental results also show good specificity against alpha 1 fetoprotein and L-tryptophan disruptors.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Huanyu Yang,Zhiping Sun,Xinguang Qin,Hongyuan Wu,Haizhi Zhang,Gang Liu
Ultrasmall Au nanoparticles (UsAuNPs) in the size range of 4.0–7.0 nm was successfully immobilized on the surface of 2D metalloporphyrinic metal–organic framework nanosheets (2D MOF). Firstly, The obtained hybrid nanomaterial, UsAuNPs/2D MOF, was fully characterized by TEM, HRTEM, element mapping images and XPS. Then, the peroxidase-like activity of UsAuNPs/2D MOF was comparatively studied with other hybrid nanozyme to explore the influence of AuNPs size on peroxidase-like activity. Further, UsAuNPs/2D MOF with outstanding peroxidase-like activity was selected to form ternary cascade enzyme reaction with acetylcholinesterase (AChE) and choline oxidase (ChOx). Based on the inhibitory effect of organophosphorus pesticides on AChE, a fast and sensitive colorimetric method was established for trichlorfon detection with the advantages of simple operation, low detection limit (1.7 μM), good linear range (1.7–42.4 μM) and high accuracy (recovery rate of 96.6–105.3%). Finally, this method was applied to visual analysis of trichlorfon concentration in tomatoes, cucumbers and eggplants.
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高影响因子
APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Yanling Zhang,Min Liu,Shuai Pan,Lulu Yu,Shaoshuai Zhang,Ruijiang Liu
Based on magnetic Fe 3 O 4 /Fe 2 O 3 @Au nanoparticles (Fe 3 O 4 /Fe 2 O 3 @Au NPs), to detect an important tumor screening marker, vascular endothelial growth factor 165 (VEGF 165 ), a label-free electrochemical aptasensor was constructed. Magnetic glassy carbon electrode (MGCE) after Fe 3 O 4 /Fe 2 O 3 @Au NPs surface modification by a magnetic-induced self-assembly technique was the platform. Fe 3 O 4 /Fe 2 O 3 @Au NPs were used as the signal amplifier to enhance electron transfer, which was essential for the detection of trace amounts of biological analytes, and were prepared via heating hydrolyzation-combustion calcination and trisodium citrate reduction. Meanwhile, aptamers bound to the as-prepared nanomaterial via Au-s bonds and specifically recognized VEGF 165. The electrochemical properties of the aptasensor were evaluated with [Fe(CN) 6 ] 3−/4 − as redox probes. When in optimal detection conditions, an excellent linear relationship (between 0.01 and 10 pg mL −1 , R 2 = 0.9973) was obtained for the electrochemical aptasensor. The limit of detection (LOD) and the limit of quantitation (LOQ) of the electrode were 0.01 and 0.03 pg mL −1 . This proposed aptasensor exhibited favourable selectivity, stability, reproducibility, and regenerability. The results demonstrated that the sensor was promising to provide an efficient, accurate, and low-cost auxiliary detection method for the early screening of VEGF 165 -related diseases, which had important clinical practical value for the research of human precision medicine.
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TALANTA
·
2020
·
IF 5.339
Bingxue Hu,Da-Wen Sun,Hongbin Pu,Qingyi Wei
A universal surface-enhanced Raman scattering (SERS) substrate with tuneable plasmonic behaviour and long-term stability was synthesized and characterized, and the substrate was applied in the sensitive detection of fungicide malachite green (MG) in fish fillets. Temperature-responsive poly (N-isopropyl acrylamide) (pNIPAM) template and anisotropic Au nanorods (NRs) were synthesized to prepare pNIPAM @ Au NRs nano-hybrids with dynamically optical and SERS properties and high stability. Results showed that excitation wavelength-dependent SERS efficiency could be tailored by temperature-induced swelling and collapse of the nano-hybrids, thus anticipated optical and SERS properties of the composites could be obtained. Furthermore, the SERS performance, uniformity, long-term stability analysis, and MG in fish evaluation showed that the detection of MG in fish tissues was realized with a limit of detection of 1.58 × 10 −9 M (0.73 ng/g), and the pNIPAM @ Au NRs composites could serve as effective SERS substrates allowing trace level detection of contaminants for the food industry.
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高影响因子
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