DRUG DEVELOPMENT RESEARCH
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2026
·Vol. 87
·期 1
·e70224
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IF 4.2
Sitao Liang,Yonghua Zhu,Jiahao Su,Chuangcai Luo,Chunhong Yang
Mitochondrial dynamics play a crucial role in glioma progression by regulating cellular metabolism, proliferation, and survival. This study investigated the effects of desmopressin (dDAVP), a synthetic vasopressin analog, on mitochondrial function in human U87 MG glioma cells. Our results demonstrate that dDAVP treatment induces dose-dependent cytotoxicity while upregulating vasopressin type 2 receptor expression. The compound significantly increased oxidative stress markers and impaired mitochondrial function, as evidenced by reduced ATP production, compromised respiratory chain activity, and decreased oxygen consumption. Furthermore, dDAVP promoted mitochondrial fragmentation through Drp1 activation, enhancing its phosphorylation at Ser616 and subsequent mitochondrial translocation. Mechanistically, dDAVP was found to activate CaMKII signaling, which mediated the observed changes in Drp1 phosphorylation and mitochondrial dynamics. The CaMKII inhibitor KN-93 effectively reversed dDAVP-induced mitochondrial fragmentation, Drp1 phosphorylation, and energy metabolism impairment. The AVPR2 antagonist tolvaptan blocked dDAVP effects, confirming receptor specificity. These findings reveal that dDAVP disrupts mitochondrial homeostasis in glioma cells through AVPR2-mediated CaMKII-dependent regulation of Drp1 activity, leading to mitochondrial dysfunction and cell damage. The study provides new insights into the molecular mechanisms underlying dDAVP's effects on glioma cells and suggests potential therapeutic applications targeting the CaMKII-Drp1 axis in glioma treatment.
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Advanced Science
·
2026
·
IF 14.1
Ri Wang,Xiaofei Deng,Bo Chen,Jienan Shen,Wei Xu,Yingjie Zhu,Yi Zhang,Hui Yang
Illicit drug abuse presents a global challenge to public health and security, creating an urgent need for reliable detection methods to curb trafficking and mitigate societal harm. Methamphetamine (METH), a highly addictive and widely circulated stimulant, requires urgent monitoring solutions. We introduce an approach for the rapid and direct detection of METH using an electrochemical sensor enhanced with MXene nano-interfaces that operates on the principle of electrochemical oxidation. Theoretical simulations are conducted to elucidate the reaction pathway of METH and to analyze the molecular interactions between METH and the MXene surface, providing key insights into the underlying molecular dynamics. The rich surface functionalities of MXene enable a favorable structural affinity with METH, facilitating enhanced interfacial interactions that promote oxidation kinetics and amplify electrochemical responses. The sensor demonstrates a linear response to METH across a concentration range from 2 ngmL −1 to 50 µg mL −1 , alongside satisfactory anti-interference performance and repeatability, rendering it appropriate for on-site preliminary screening of positive samples. Its successful validation in complex biological matrices confirms its broad applicability. By combining user-friendly operation with a rapid response, this method establishes a robust theoretical framework and a practical solution for the on-site screening of METH and related illicit substances.
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CANCER RESEARCH
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2026
·
IF 16.6
Liao Fangli,Tong Yanran,Sun Hua,Chen Sen,Wen Siyang,Du Yan-e,Jiang Linshan,Huang Tong,Liu Manran,Chen Weixian,Yang Liping
The immunosuppressive tumor microenvironment (TME) enables cancer cells to evade clinical immunotherapies. Neural networks are vital components of the TME, and interactions between cancer cells, neuronal cells, and immune cells mediate immunosuppression. Hence, understanding the mechanisms of intercellular crosstalk could inform immunomodulatory approaches to enhance immunotherapy efficacy. Here, we found that the vagus nerve regulated the crosstalk between gastric cancer (GC) cells and group 3 innate lymphoid cells (ILC3s), boosting immune resistance in GC by enhancing programmed death ligand 1 (PD-L1) expression. Specifically, the infiltrated vagus nerve released acetylcholine (ACh) that elevated the expression of lipase ABHD16A in GC cells, facilitating the production and secretion of the metabolite lysophosphatidylserine (LysoPS) into the TME. LysoPS facilitated the proliferation and activation of ILC3s in TME, resulting in production of the cytokine interleukin (IL)-22 via the GPR34/AKT/STAT3 axis. In turn, IL-22 triggered the unfolded protein response (UPR) in GC cells, which led to an increase in PD-L1 expression that enhanced immune resistance. Importantly, targeting ACh or the crosstalk between GC cells and ILC3s significantly enhanced the efficacy of anti-PD-L1 immunotherapy. Serum levels of LysoPS and IL-22 were elevated in GC patients, particularly those with perineural invasion. Collectively, these findings provide valuable insights into the crosstalk between GC cells, nerve cells, and ILC3s that regulates immunosuppression and response to ant-PD-L1 immunotherapy, emphasizing the potential clinical significance of this axis for detecting and treating GC.
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Food Chemistry-X
·
2026
·
IF 8.2
Qingyang Zhang,Meiyu Zheng,Zhen Lu,Kangyu Zhao,Shu Wang,Bin Li,Zhigang Hu,Sihong Zhang,Dongping He,Fenfen Lei,Li Zhou
Emulsification was found to enhance the nutritional value and flavor of bovine bone broth, although the specific processing conditions and their impact on amino acid profiles and flavor compounds remained unclear. To address this knowledge gap, this study identified critical parameters for producing clear broth (simmering time of 4 h, temperature of 115 °C, bone size of 4.0 cm, and liquid-to-solid ratio of 1:4) and established optimal conditions for white broth (a shear rate of 5000 r/min, emulsification pressure of 18 kPa, temperature of 50 °C, and duration of 90.0 min). Analysis identified 17 amino acids in both broths, while volatile compound profiling revealed 57 and 64 distinct compounds in the clear and white broths, respectively. The findings provide a theoretical foundation for the high-value utilization of bovine bones.
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Results in Engineering
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2026
·
IF 7.9
Qian-Qian Chu,Jishuang Luan,Dehu Qv,Yangfan He,Peitao Fan,Xuanbo Wang,Bo Cheng,Li Feng,Baizeng Fang
Phase change materials (PCMs) absorb and release energy from the environment effectively during the phase change process, leading to their widespread use in the field of thermal energy storage. However, the development of PCMs has been limited by issues such as poor thermal conductivity and light absorption. We therefore designed a silver-plated melamine foam (MF) framework and synthesized a phenolamine aggregate coating on the MF through a redox reaction, enhancing the bonding with silver nanoparticles and resulting in fully silver-plated MF. Meanwhile, hydroxylated carbon nanotubes (HCNTs) were added to the framework as a filler. This modification improved the conductivity of the corresponding polyethylene glycol (PEG)-based composite PCMs from 0.33 (pure PEG) to 0.82 W m⁻¹ K⁻¹ due to the construction of a multilevel thermal conduction channel. Additionally, a high photothermal conversion efficiency of 92.4 % was achieved through the combined effect of the local resonance absorption of the silver and the molecular thermal vibration of the HCNTs. These advances provide a new strategy for optimizing the performance of PCMs, with potential applications in energy storage and thermal management.
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Journal of Materials Chemistry B
·
2026
·
IF 5.7
Linyu Wang,Jiafan Bai,Xiangli Chen,Hongyan Liu,Jie Shi,Wenzhen Peng,Jie Weng,Wei Zhi,Jianxin Wang
Colorectal cancer (CRC) chemotherapy faces challenges such as poor gastrointestinal stability, low targeting efficiency, severe toxicity, and complex protocols. Recent pH-responsive nanocarriers mainly improve environmental stability but lack intelligent control. This study introduces a novel two-step "one-pot" aqueous synthesis strategy to create dual-targeting core-shell nanoparticles (LTDR-DS NPs) that are both efficient and environmentally friendly. The core contains a lysine-tannic acid conjugate and D-galactose, while the shell is a pH-responsive dopamine-alginate sodium (DA-SA) "smart armor." This design enables spatiotemporal targeting, combining pH responsiveness, precise delivery, and multi-mechanistic synergy.Unlike traditional nanocarriers, LTDR-DS NPs co-optimize stability and targeting, overcoming the dual challenges of poor stability and low targeting efficiency. They offer a groundbreaking, low-toxicity treatment strategy with high potential for clinical translation, enhancing therapeutic efficacy while reducing systemic toxicity and advancing CRC chemotherapy.
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DALTON TRANSACTIONS
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2026
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IF 3.3
Xingyu Wang,jiahui zhang,Chengyang Fang,Xinyi Shi,jiaping zhao,Wenchu Lin,Hui Wang,Rongrong Nie
Metal-free carbon-based nanomaterials hold great potential for imaging-guided cancer therapy. In this work, we synthesized carbon quantum dots embedded graphitic carbon nitride nanofibers (GCN-CQD) via a facile one-pot solvothermal approach for fluorescent imaging and combined cancer therapy. Embedded CQDs impact excitation-dependent-tunable and upconverted fluorescence to the nanofibers, establishing them as robust optical contrast agents suitable for multicolor confocal imaging. Within the tumor microenvironment (TME), GCN-CQD could catalyze hydrogen peroxide decomposition to generate hydroxyl radical (·OH) enabling chemodynamic therapy (CDT). Furthermore, GCN-CQD efficiently adsorb near-infrared (NIR) light, converting it locally into heat for photothermal therapy (PTT) while simultaneously generating singlet oxygen (1O2) to drive photodynamic therapy (PDT), collectively inducing cells death. This work offers novel perspectives for developing multimodal combination therapy approaches utilizing metal-free carbon-based nanoplatforms.
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Materials Horizons
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2026
·
IF 10.7
Kang Wang,Hengjie Zhang,Mengxin Wang,Zhengyong Li,Wu Wu,Peng-cheng Liu,Ruiqi Liu,Zhipeng Gu,Yiwen Li,Zhenyu Zhang
Achieving scarless healing combine with skin appendage regeneration remains an extremely challenging task in the treatment of skin wounds. Vitamin A derivatives have shown great potential for follicle neogenesis and scarless repair but suffer from poor solubility, instability, photosensitivity, and toxicity in wound healing process. Here, we present a multifunctional bioadhesive hydrogel system that integrates polydopamine (PDA) nanoparticles with Vitamin A derivatives to promote functional skin regeneration. The PDA nanoparticles stabilized and enabled pH-responsive release of Vitamin A derivatives, while simultaneously providing reactive oxygen species (ROS) scavenging and antioxidant protection. Embedded within an imine-crosslinked adhesive hydrogel network, this platform achieved strong tissue adhesion, sustained drug delivery, and favorable immune microenvironment modulation. In vivo, using both rats burn wounds and rabbit hypertrophic scar models, the optimized formulation accelerated wound closure, rebalanced collagen deposition, suppressed myofibroblast activation, and markedly prevented pathological fibrosis. Strikingly, it also induced robust de novo hair follicle formation, indicating true functional tissue restoration. Transcriptomic and immunofluorescence analyses further revealed downregulation of pro-fibrotic and inflammatory pathways alongside activation of regenerative signaling, including M2 macrophage polarization and suppression of M1 phenotype in treated wounds. This work introduces an early Vitamin A-based nanocomposite hydrogel with dual anti-scarring and regenerative functions, offering a promising strategy for advanced wound care.
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EXPERIMENTAL CELL RESEARCH
·
2026
·
IF 3.5
Fang Tong,Xue Gao,Yuhong Shang
Endometrial cancer (EC) represents one of the most prevalent malignancies of the female reproductive system. Ferroptosis, a recently identified form of programmed cell death, has garnered increasing attention in cancer research. Ubiquitin-specific peptidase 18 (USP18), a member of the deubiquitinating enzyme family, has been recognized as an oncoprotein in various cancers; however, its functional role and underlying mechanisms in EC remain largely unexplored. In this study, we found that USP18 was markedly upregulated in EC patients (n=15, stage I; n=4, stage II and n=1, stage III), and elevated USP18 expression correlated with unfavorable prognosis. Functional assays demonstrated that knockdown of USP18 significantly inhibited EC cell proliferation. Moreover, USP18 silencing promoted the accumulation of lipid reactive oxygen species (ROS), malondialdehyde (MDA), and Fe 2+ , thereby enhancing erastin-induced ferroptosis. In contrast, USP18 overexpression produced opposing effects. These in vitro findings were further validated in vivo, where USP18 knockdown suppressed tumor growth and promoted ferroptosis. Mechanistic investigations revealed that USP18 interacted with and deubiquitinated histone deacetylase 3 (HDAC3), thus leading to its stabilization. Subsequent rescue experiments confirmed that the tumor-promoting effects of USP18 were abrogated upon HDAC3 knockdown. Taken together, our results identify the USP18/HDAC3 axis as a key regulator of EC cell proliferation and ferroptosis suppression, underscoring the potential of USP18 as a therapeutic target in EC.
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Chinese Medicine
·
2026
·Vol. 21
·期 1
·40
·
IF 5.7
Yao Qianfang,Hu Guishan,Yin Can,Wu Anguo,Hu Guangqiang,Qin Dalian,Zhou Xiaogang,Law Betty Yuen-Kwan,Du Xi,Chen Li,Li Jianqiao,Lin Hong,Long Xin,Wu Jianming,Yu Lu
Background Cerebral ischemia/reperfusion injury (CI/RI) remains a critical barrier to effective ischemic stroke (IS) treatment. While mitophagy activation has been shown to attenuate apoptosis and pyroptosis, thereby ameliorating CI/RI, the therapeutic potential of natural compounds targeting this pathway remains underexplored. Penthorum chinense Pursh (PCP), a traditional hepatoprotective herb, contains Thonningianin A (TA), a bioactive compound with reported autophagic properties. However, the role and mechanisms of TA in CI/RI mitigation remain unclear. Methods In vivo, a middle cerebral artery occlusion/reperfusion (MCAO/R) rat model was established to evaluate TA’s neuroprotective effects via TTC staining, Longa neurological scoring, and immunofluorescence staining. In vitro, oxygen–glucose deprivation/reoxygenation (OGD/R)-treated HT22 and BV2 cells were used to assess TA’s impact on cell viability (MTT, Hoechst/PI staining), mitochondrial oxidative stress (DHE, TMRM, JC-1, Mito-Tracker staining and Western blot), apoptosis (flow cytometry, immunofluorescence staining, Hochest and PI staining and Western blot), and pyroptosis (EthD-2/YO-PRO-1 staining and Western blot). Autophagy and mitophagy modulation was investigated using rapamycin (Rap), 3-MA (autophagy inhibitor), CCCP (mitophagy inducer), and AC220 (mitophagy inhibitor) in EGFP-LC3-U87 and mCherry-GFP-FIS1-293T cells. Co-localization immunofluorescence and Western blotting were employed to validate PINK1/Parkin pathway involvement. Results TA administration significantly improved neurological function, reduced cerebral infarct volume, and attenuated neuronal damage in MCAO/R rats. In vitro, TA suppressed OGD/R-induced mitochondrial oxidative stress and apoptosis in HT22 cells while mitigating pyroptosis in BV2 microglia. Mechanistically, TA activated PINK1/Parkin-dependent mitophagy, as evidenced by enhanced LC3-II/I ratio, and increased mitochondrial-autophagosome co-localization. Crucially, TA’s anti-apoptotic and anti-pyroptotic effects were abolished upon mitophagy inhibition. These findings were corroborated in the MCAO/R model, where TA upregulated PINK1/Parkin signaling and mitigated cell damage. Conclusion This study identifies TA as a novel natural agent alleviating CI/RI by activating PINK1/Parkin-mediated mitophagy, thereby concurrently suppressing apoptosis and pyroptosis. These findings provide the first elucidating the molecular mechanis underlying TA's potential as a therapeutic candidate for IS. Graphical
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
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IF 12.6
Chaoran Liu,Wenyu Zhang,Qingbin He,Xinyu Ma,Jianfeng Qiu,Runxiao Zheng,Hongjin Xue
Catalyzing the glucose cascade reaction to impair tumor cell energy metabolism represents a promising strategy for tumor therapy. However, the application of natural enzymes as therapeutic agents remains limited by various challenges. Although nanozymes with multi-enzyme activities, including glucose oxidase-like (GOx-like) activity, have been reported, their development often involves complex material combinations and cumbersome synthesis processes. Here, we develop a nanozyme with GOx-, peroxidase (POD)-, superoxide dismutase (SOD)-, and NADH oxidase (NOX)-mimic activities by simply controlling the AuPt alloy ratio. The optimal cascade activity was observed for the nanozyme at an Au: Pt proportion of 13:7. Density functional theory (DFT) calculations revealed that Au sites drive glucose dehydrogenation (GOx-like), while Pt sites facilitate hydroxyl radical (•OH) generation (POD-like). Both in vitro and in vivo data indicated that Au 13 Pt 7 nanozymes disrupt tumor redox/metabolic homeostasis by depleting glucose and generating cytotoxic •OH, and impairing mitochondrial function via NOX-like, thereby inducing cell apoptosis. Notably, apoptotic immunogenic cell death (ICD) induces antitumor immunity and suppresses tumor metastasis. This study presents an innovative strategy for engineering nanozymes with multi-enzyme catalytic capabilities while demonstrating the promising application of alloy-based nanozymes in synergistic metabolic-immunotherapy.
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Pharmaceutics
·
2026
·Vol. 18
·期 1
·98
·
IF 5.5
Jingjing Wang,Rui Ni,Ziwei Li,Jianhong Chen,Yao Liu
Background/Objectives:Wound healing represents a pervasive and urgent clinical challenge. Hard-to-heal chronic wounds are frequently complicated by infections, inflammatory responses, and oxidative stress. Currently, wound dressings are broadly categorized into dry and moist types, with moist wound dressings for chronic wounds accounting for approximately 70% of market revenue. Recently, adipose-derived stem cells (ADSCs), which possess self-renewal and multi-lineage differentiation capabilities, have emerged as a promising strategy for promoting tissue regeneration and wound repair.Methods:In this study, we developed a novel luteolin nanoparticle–ADSCs composite hydrogel (GelCA@LUT@ADSCs). This system was constructed by first encapsulating ADSCs within a chitosan/alginate hydrogel (GelCA), followed by coating the hydrogel with luteolin-loaded nanoparticles (LUT@NPs).Results:The sustained release of LUT@NPs from the hydrogel modulates the wound microenvironment, enhancing the pro-healing functions of ADSCs at the wound site. The GelCA hydrogel exhibited excellent biocompatibility. Both in vitro and in vivo results demonstrated that GelCA@LUT@ADSCs treatment effectively reduced inflammation, promoted angiogenesis and collagen deposition, stimulated cell proliferation and migration, and polarized macrophages toward an anti-inflammatory, pro-healing M2phenotype, thereby accelerating wound healing.Conclusions:Overall, this innovative therapeutic approach provides a novel strategy for wound management through a synergistic division of labor between pharmaceutical agents and stem cells.
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