BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Jing-Yi Huang,Lang Zhao,Wan Lei,Wei Wen,Yi-Jia Wang,Ting Bao,Hua-Yu Xiong,Xiu-Hua Zhang,Sheng-Fu Wang
In this work, we have developed an electrochemical aptasensor for high-sensitivity determination of carcinoembryonic antigen (CEA) based on lead ion (Pb 2+ )-dependent DNAzyme-assisted signal amplification and graphene quantum dot-ionic liquid-nafion (GQDs-IL-NF) composite film. We designed hairpin DNA containing CEA-specific aptamers and DNAzyme chains. In the presence of CEA, hairpin DNA recognized the target and performed a DNAzyme-assisted signal amplification reaction to yield a large number of single-stranded DNA. The GQDs-IL-NF composite film was immobilized on the glassy carbon electrode for the interaction with single-stranded DNA through noncovalent π-π stacking interaction. Therefore, the methylene blue-labeled substrate DNA (MB-substrate) was fixed on the electrode and exhibited an initial electrochemical signal. Under optimal conditions, the response current change was proportional to the concentration of CEA, demonstrating a wide linear range from 0.5 fg mL −1 to 0.5 ng mL −1 , with a low detection limit of 0.34 fg mL −1 . Furthermore, the proposed aptasensor was successfully applied in determining CEA in serum samples, showing its superior prospects in clinical diagnosis.
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高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2016
·Vol. 39
·期 16
·3221-3229
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IF 2.741
Qiong Jiang,Qin Liu,Qiliang Chen,Wenjie Zhao,Guoqiang Xiang,Lijun He,Xiuming Jiang,Shusheng Zhang
Magnetic particles modified with a dicationic polymeric ionic liquid are described as a new adsorbent in magnetic solid-phase extraction. They were obtained through the copolymerization of a 1,8-di(3-vinylimidazolium)octane-based ionic liquid with vinyl-modified SiO 2 @Fe 3 O 4 , and were characterized by FTIR spectroscopy, X-ray diffraction, and vibrating sample magnetometry. The modified magnetic particles are effective in the extraction of organophosphate pesticides and polycyclic aromatic hydrocarbons. Also, they can provide different extraction performance for the selected analytes including fenitrothion, parathion, fenthion, phoxim, phenanthrene, and fluoranthene, where the extraction efficiency is found to be in agreement with the hydrophobicity of analytes. Various factors influencing the extraction efficiency, such as, the amount of adsorbent, extraction, and desorption time, and type and volume of the desorption solvent, were optimized. Under the optimized conditions, a good linearity ranging from 1–100 μg/L is obtained for all analytes, except for parathion (2–200 μg/L), where the correlation coefficients varied from 0.9960 to 0.9998. The limits of detection are 0.2–0.8 μg/L, and intraday and interday relative standard deviations are 1.7–7.4% ( n = 5) and 3.8–8.0% ( n = 3), respectively. The magnetic solid-phase extraction combined with high-performance liquid chromatography can be applied for the detection of trace targets in real water samples with satisfactory relative recoveries and relative standard deviations.
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BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Yanyan Yu,Chao Yu,Tianxiao Yin,Shanshan Ou,Xiaoyu Sun,Xiangru Wen,Lin Zhang,Daoquan Tang,Xiaoxing Yin
An efficient ratiometric electrochemical biosensor for Cu 2+ determination was constructed using dual hydroxyl-functionalized poly (ionic liquid) (DHF-PIL) as the catalyst support. The DHF-PIL exhibited typical macroporous structure, which provided a high surface area of 39.31 m 2 /g for the sufficient loading of biomolecules . The specific recognition of Cu 2+ was accomplished by employing neurokinin B (NKB) for the first time, which could bind to Cu 2+ to form a [Cu II (NKB) 2 ] complex with high specificity. Meanwhile, a common redox mediator , 2, 2′-Azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) was modified into DHF-PIL by electrostatic interactions to act as an inner reference molecule, which provided a built-in correction for environmental effects and improving the detection accuracy. With this strategy, the developed electrochemical biosensor was capable of determining Cu 2+ with a linear range between 0.9 and 36.1 μM and low detection limit (LOD) and quantification limit (LOQ) of 0.24 and 0.6 μM, respectively. The sensor also displayed a satisfactory selectivity against a series of interferences in the brain, including metal ions , amino acids and other endogenous compounds . Accordingly, the present biosensor was successfully applied to evaluate Cu 2+ levels in normal and AD rats.
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ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Lei Mao,Yaxin Chen,Ruifang Zhang,Chun Yang,Yanhong Zhou,Guangya Zhang
The utilization of carbonic anhydrases (CAs) for CO2 capture aligns with the principles of environmental sustainability. However, there is an urgent need to minimize the cost of CA purification while improving their performance in harsh environments. A novel strategy was proposed to solve the above problems by employing the ferritin-tagged CA variant (DvCA8.0-F). The purification is achieved by low-speed centrifugation, yielding 92% activity recovery and 95% purity. After 14 weeks of incubation in a 50% MDEA solution at 50 °C, DvCA8.0-F maintained activity nearly equivalent to the initial activity, which showed remarkable stability compared to the free ones. Encouragingly, DvCA8.0-F had a yield of up to 800 mg/L, allowing for cost-effective CA-assisted CO2 absorption and desorption in MDEA solutions. The results show that DvCA8.0-F reduced the absorption time from 70 to 50 min at 40 °C and desorption time from 40 to 25 min at 96 °C in 25%MDEA+1%[N1111][Gly] solution with a final CO2 load and a stripped amount of CO2 at the same time. DvCA8.0-F exhibits the maximum reaction rate at 96 °C; the CO2 regeneration efficiency improved from 60% at 87 °C to 72%. When the concentration of DvCA8.0-F was 1.5 g/L, the absorption rate of CO2 reached about 90% of that of the 25% MEA solution. These results demonstrate the simplicity of preparation and the outstanding properties of DvCA8.0-F, which paves a feasible way for CA-assisted CO2 capture. This exciting strategy may also have excellent potential in enzymes for innovative chemical synthesis to achieve the sustainable development of green chemistry.
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JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 14
·2992-2998
·
IF 2.557
Xinpei Li,Ying Sun,Qun Sun,Li Liang,Huilan Piao,Yanxiao Jiang,Aimin Yu,Daqian Song,Xinghua Wang
Zinc oxide nanoparticles have recently been used as effective adsorbent materials for sample pretreatment in analytical chemistry because of their excellent properties, such as high specific surface area, high effective porosity, non-toxicity, and ease of fabrication. In this study, the zinc oxide nanoparticles functionalized by an ionic liquid, 1-carboxyethyl-3-methylimidazolium chloride, were fabricated and used as the adsorbent for the solid phase extraction of five triazine herbicides in corn for the first time. High-performance liquid chromatography was employed for the determination of these triazine herbicides. Several experimental parameters affecting the extraction efficiency were investigated, including the volume of extraction solvent, the extraction time, the type of extraction solvent and elution solvent, the amount of absorbent, and the volume of elution solvent. By using the proposed method, low limits of detection and quantification for all the five triazine herbicides were obtained between 0.71–1.08 and 2.67–3.64 ng/g, respectively. Recoveries of the proposed method range from 89.05 to 100.33% with intra- and inter-day relative standard deviations lower than 8.45%. The calibration curves are linear in the concentration range of 0.005–1.00 μg/g with the correlation coefficient higher than 0.9954.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xuyan Wei,Tingrui Lin,Jiyuan Gao,Yang Hu,Zeyu Zhang,Jing Peng,Jiuqiang Li,Maolin Zhai
Flexible electronic devices, such as supercapacitors (SCs), place high demands on the mechanical properties, ionic conductivity, and electrochemical stability of electrolytes. Hydrogels, which combine flexibility and the advantages of both solid and liquid electrolytes, will meet the demand. Here, we report the synthesis of novel poly(ionic liquid)/polyacrylamide double-network (DN) (PIL/PAM DN) hydrogel electrolytes containing different metal salts via a two-step γ-radiation method. The resultant Li2SO4-1.0/PIL/PAM DN hydrogel electrolyte possesses excellent mechanical properties (tensile strength of 3.64 MPa, elongation at break of 446%) and high ionic conductivity (24.1 mS·cm–1). The corresponding flexible SC based on the Li2SO4-1.0/PIL/PAM DN hydrogel electrolyte (SC-Li2SO4) presents improved ion diffusion, ideal electrochemical double-layer capacitor behavior, good rate capability, and excellent cyclic stability. Moreover, symmetric SC-Li2SO4 achieves a wide operating voltage range of up to 1.5 V, with a maximum energy density of 26.0 W h·kg–1 and a capacitance retention of 94.1% after 10,000 galvanostatic charge–discharge cycles, owing to the deactivation of free water molecules by the synergistic effect of PIL, PAM, and SO42–. Above all, the capacitance of SC-Li2SO4 is well-maintained after overcharge, overdischarge, short circuit, extreme temperature, compression, and bending tests, indicating its high security and flexibility. This work reveals the enormous application potential of PIL-based conductive hydrogel electrolytes for flexible electronic devices.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Gongheng Zhang,Abdullah Ali Mohammed Ahmed Al-Anzah,Fengxiang Yin,Jie Zhang,Guoru Li,Biaohua Chen,Qiuyue Gao,Xian Tong Yu,Yuqian Sun
The precursor ZIF-67/xC-T was prepared by solvothermal and pyrolysis methods. A series of ZIF-67/xC-T-yIL catalysts were then prepared by modifying the surface of the precursor with [BMIM][NTf 2 ] ionic liquid . The results showed that [BMIM][NTf 2 ] as sulfur and nitrogen sources, N, S co-doped carbon can improve the catalytic activity of ORR . Meanwhile, [BMIM][NTf 2 ] formed an oxygen-rich layer on the catalyst surface, which increased the contact between oxygen and the active sites, reduced the alkaline strength of the catalyst surface, and improved the desorption ability of the catalyst to the product. Therefore, the ZIF-67/xC-T-yIL catalyst exhibited better ORR activity in an alkaline electrolyte than the catalyst without [BMIM][NTf 2 ]. Among the ZIF-67/xC-T-yIL catalysts, ZIF-67/1.2 C-600–6IL demonstrated the best ORR catalytic activity, with a half-wave potential of 0.86 V and a limiting current density of 5.21 mA cm −2 in 0.1 mol L −1 KOH, which was comparable to that of commercial 20% wt% Pt/C.
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CHROMATOGRAPHIA
·
2018
·Vol. 81
·期 4
·595-610
·
IF 1.401
Liu Wei,Quan Ji
In this work, a quantitative structure–property relationship (QSPR) model based on atom-type electrotopological state (E-state) indices has been developed for predicting the dispersive liquid–liquid microextraction (DLLME) enrichment effect for aromatic organics by using 1-octyl-3-methylimidazolium hexafluorophosphate ([OMIM][PF 6 ]) ionic liquid as extractant. The enrichment factors of the aromatic organics obtained through DLLME-IL process were used as the dependent variable of the multiple linear regression (MLR) equation. The E-state indices of those organic compounds which combined together both electronic and topological characteristics of the analyzed molecules were used as molecular structure descriptors and the independent variable of the equation. The squared correlation coefficient R 2 for the MLR was 0.992 for the training set of 33 compounds. For the test set of 8 compounds, the linear regression coefficient was R 2 = 0.985. The results of this study showed that it can successfully predict enrichment factors of [OMIM][PF 6 ] in DLLME by using atom-type E-state indices, which can provide one more way for predicting the ability of ionic liquid to extract organic compounds based on their molecular structures.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Liwei Qian,Yuhao Zhang,Wenqi Song,Sufeng Zhang,Nan Zhang,Valentin Nica
Glycoproteins are essential in biological systems, yet their complex structures and diverse glycosylation patterns present challenges for efficient and specific recognition. Herein, molecularly imprinted polymers (MIPs) with high selectivity and adsorption capacity for glycoproteins were developed using two synergistic strategies. One strategy was to synthesize a boronic acid-containing ionic liquid (PBA-IL) functional monomer that combines long-range non-covalent interactions with short-range covalent bonds, enhancing the interaction modes with ovalbumin (OVA) to improve imprinting efficiency. The other strategy entailed constructing a composite carrier with high specific surface area (50.3 m 2 /g) and abundant active sites by in-situ growth of UiO-66 metal–organic framework crystals on a wood-derived cellulose sponge (WCS). This composite (WCS@UiO-66) was utilized in a template immobilization method combined with surface imprinting technique (TIM-SIT) to prepare the MIPs (WCS@UiO-66@MIPs). Molecular simulations indicated that PBA-IL formed stronger interactions with OVA compared to traditional monomer. Based on these characteristics, the WCS@UiO-66@MIPs achieved rapid adsorption equilibrium within 40 min, exhibiting surprisingly high adsorption capacity (up to 571 mg g −1 ) and recognition ability (imprinting factor of 5.09) for OVA. The competitive adsorption experiments also demonstrated the efficient extraction of OVA from biological samples by WCS@UiO-66@MIPs, confirming its exceptional specificity. Additionally, the osmotic separation experiments revealed that WCS@UiO-66@MIPs achieved a selective permeation factor of up to 7.68 for OVA in a binary protein mixture, further demonstrating their potential for large-scale continuous separation processes. Therefore, this work provides a promising strategy for the efficient and specific recognition of glycoproteins, advancing their application in biotechnology and analytical chemistry.
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Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Qingyuan Xue,Linyan Cheng,Qinghua Qu,Lingwei Yang,Cheng Fang,Hongping Li,Jing Ding,Hui Wan,Guofeng Guan
Covalent organic frameworks (COFs) and ionic liquids (ILs) as raw materials exhibit effective catalytic performance and adsorption capabilities in the reaction of epoxides and atmospheric CO 2 , respectively. Nonetheless, bonding modes between ILs and COFs have restricted their utility, thereby constraining the in-depth study of synergistic catalytic mechanisms over these composite catalysts. Herein, a heterogeneous composite ([DBUH] 2 Cys@COF-X) were successfully prepared by the facile acid-base neutralization method, incorporating varying amounts of amino acid ILs ([DBUH] 2 Cys) into the pore channels of sulfonic acid-based COF (TpPa-SO 3 H). [DBUH] 2 Cys@COF-3 efficiently catalyzed epichlorohydrin with a yield of 96.38 % without any solvent or co-catalyst under atmospheric CO 2 . Notably, larger sterically hindered epoxides were selectively blocked on the surface of [DBUH] 2 Cys@COF-3, enabling [DBUH] 2 Cys@COF-3 to act as a gatekeeper for selectively catalyzing small sterically hindered epoxides due to [DBUH] 2 Cys was distributed within the pore channels of [DBUH] 2 Cys@COF-3. Through DFT calculations, comprehensive understanding of the synergistic activation conferred of -COO - and [DBUH] + towards CO 2 and epoxides in [DBUH] 2 Cys@COF-3 and the ring-opening mechanism was clarified. This work presents a fresh outlook on the catalyst design by the facile acid-base neutralization method for efficiently catalyzing small-hindered epoxides under atmospheric CO 2 .
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ELECTROCHIMICA ACTA
·
2011
·
IF 3.65
Yiping Liu,Shirong Wang,Zhongqiang Shan,Xianggao Li,Jianhua Tian,Yeming Mei,Huanmei Ma,Kunlei Zhu
The anatase TiO 2 hollow spheres with a diameter of approximately 250 nm were synthesized by a chemical template method. Compared to conventional P25 nanocrystalline particles, the TiO 2 hollow structured materials not only have a low density, high specific surface areas, and hierarchically porous structures but also exhibit high light-scattering efficiency and fast motion of the charge carriers. A photoelectrode consisting of TiO 2 hollow spheres for use in dye-sensitized solar cells (DSSCs) is prepared, and the nanostructure and the performance of DSSCs with an ionic liquid electrolyte are investigated. Improved performance of the DSSCs (Jsc = 8.0 mA cm −2 , Voc = 0.732 V, FF = 65%, and η = 3.79%) is obtained compared with the P25 TiO 2 nanocrystalline DSSCs (Jsc = 7.25 mA cm −2 , Voc = 0.702 V, FF = 59%, η = 2.96%, and measured at 100 mW cm −2 , AM 1.5 G). The improved performance is primarily due to the enhanced light scattering by the TiO 2 hollow spheres and the highly porous structure of the hollow sphere electrode, allowing the highly viscous non-volatile electrolytes to thoroughly penetrate the electrode. The results show that the structure of the TiO 2 hollow sphere photoelectrode can greatly improve the performance of the DSSCs with non-volatile electrolytes.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Qiong-Qiong Ren,Jing Wu,Wen-Chao Zhang,Chang Wang,Xin Qin,Guo-Chang Liu,Zhen-Xin Li,Yi Yu
Single-walled carbon nanotubes (SWCNTs), horseradish peroxidase (HRP), and 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM·BF 4 ) were employed to construct a cellular H 2 O 2 sensor based on direct electron transfer. At a working potential of −0.35 V, HRP-BMIM·BF 4 /SWCNTs/CFUME showed a dynamic range of up to 10.2 μM with a low detection limit of 0.13 μM (S/N = 3) and a high sensitivity of 4.25 A/M cm 2 . The apparent Michaelis-Menten constant ( K m,app ) was estimated to be as low as 15.4 μM, which suggested that HRP molecules entrapped in the BMIM·BF 4 and SWCNTs immobilized at the carbon fiber ultramicroelectrode maintained a very high affinity. Because of the extremely small dimension and low working potential, HRP-BMIM·BF 4 /SWCNTs/CFUME enabled direct amperometric real-time monitoring of H 2 O 2 in HeLa cells treated with the anticancer drug, camptothecin, without requiring complex data processing and extra surface coatings to prevent interference. HRP-BMIM·BF 4 /SWCNTs/CFUME testing clearly showed that the H 2 O 2 level significantly increased in HeLa cells under camptothecin stress. When HeLa cells were cultured in medium without camptothecin, the H 2 O 2 level remained stable during the whole measurement process. These results indicate that HRP-BMIM·BF 4 /SWCNTs/CFUME could be a powerful tool for real-time investigation of cellular H 2 O 2 level, especially under anticancer drug stress. This method could provide in-depth insights regarding the occurrence, development, and apoptosis of tumors.
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