Chinese Medicine
·
2024
·Vol. 19
·期 1
·1-24
·
IF 5.3
Cao Ting,Wang Xue-lian,Rao Jiang-yan,Zhu Hui-feng,Qi Hong-yi,Tian Zhen
Background Parkinson’s disease (PD) is a chronic neurodegenerative disorder that currently has no curable strategies. More and more evidence suggests that endoplasmic reticulum (ER) stress plays an essential role in PD pathogenesis. Periplaneta americana L. ( P. americana ) is a traditional Chinese medicine with diverse therapeutic properties. This study aims to investigate the neuroprotective effect and underlying mechanism of P. americana in in vitro and in vivo PD models. Methods The exposure of SH-SY5Y cells to 1-methyl-4-phenyl-pyridinium (MPP + ) was used as the in vitro PD model. MTT assay, Hoechst staining, Calcein AM-PI staining and flow cytometry were performed to measure the cell viability and apoptosis. DCFH-DA and JC-1 assay were used to measure the intracellular ROS and mitochondrial membrane potential (Δψm), respectively. Western-blot and immunostaining were conducted to detect the expression of key molecules related with ER stress. For the in vivo PD model induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydro-pyridine (MPTP), the motor function of mice was assessed by behavioral tests, the level of TH was examined by western-blot and immunostaining, the expression of key molecules related with ER stress was measured by western-blot. Results Periplaneta americana ethanol extract (PAE) concentration-dependently inhibited MPP + -induced cell loss and increased cell viability. PAE also remarkably attenuated ROS accumulation, the decline of Δψm as well as the excessive ER stress. The neuroprotective effects of PAE could be blocked by ROS inducer trimethylamine N-Oxide or ER stress activator tunicaymycin, while the antioxidant N-Acetyl-L-cysteine or ER stress inhibitor sodium 4-phenylbutyrate mimicked the effects of PAE. Furthermore, we found that PAE could activate AKT/GSK3β/β-catenin pathway. The effect of PAE on ROS production, Δψm and ER stress was blocked by AKT inhibitor MK-2206. In in vivo model, PAE significantly improved motor function, prevented dopaminergic neuronal loss and attenuated ER stress in substantia nigra and striatum of MPTP-treated mice. Similarly, the effects of PAE on MPTP-treated mice were also abolished by MK-2206. Conclusions Our results suggest that P. americana exerts neuroprotective effects through inhibiting ER stress via AKT-dependent pathway. Periplaneta americana may represent a promising therapeutic agent for PD treatment and is worthy of further being exploited.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jiahui Du,Le Du,Jiushi Guo,Tao Sun,Linze Liu,Xueyan Yun,Tungalag Dong
Chilled beef is a perishable food product. Due to the broad-spectrum antimicrobial properties of chitosan, and the biodegradability of PLLA, chitosan and PLLA are introduced double bonding groups to obtain Chitosan- graft -Glycidyl methacrylate (Cs-g-GMA) and Poly(L-lactic acid)- co -poly(butylene itaconate) (PLBI), respectively. Cs-g-GMA is then grafted onto the surface of PLBI films by UV curing to produce PLBI/Cs-g-GMA films with non-migratory, broad-spectrum antimicrobial, biodegradable, and possess good oxygen barrier properties, compared to current antimicrobial films. Antimicrobial tests in vitro confirmed that PLBI/Cs-g-GMA film could effectively inhibit the growth and reproduction of P. fluorescens , S. aureus , and E. coli . The PLBI/Cs-g-GMA films were further used for the packaging of chilled beef during 15 days of refrigerated storage. The microbiological (total viable counts (TVC), microbial diversity analysis), physicochemical (pH, total volatile basic nitrogen (TVB-N), drip loss, color) and sensory of beef were measured to evaluate the preservation effect. The results showed that PLBI/Cs-g-GMA films can significantly inhibit Pseudomonas and Aeromonas at the phylum and genus levels, respectively. Based on the good results of the preservation index, PLBI/Cs-g-GMA film could delay the quality deterioration of chilled beef by 3 to 9 days, suggesting that its considerable prospects for maintaining the storage quality of chilled beef.
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Materials Today Chemistry
·
2025
·
IF 6.7
Haitham Saad Al-mashriqi,Yunyun Zhang,Jia Chen,Eskandar Qaed,Xin Li,Yali Yang,Hongdeng Qiu
While chiral nanomaterials have seen significant advances, chiral carbon dots (CDs) remain relatively unexplored. Chiral fluorescent probes, known for their high sensitivity and selectivity, hold great potential in biosensing and bioimaging applications. In this research, we synthesized chiral carbon dots (b-CDs and y-CDs), with the y-CDs displaying strong fluorescence, excellent water solubility, chirality-dependent optical properties, and a quantum yield of 89.87 %. The y-CDs served as a versatile probe for the highly selective detection of L-cysteine using the y-CDs + Fe 3+ system, while y-CDs alone were employed to detect ofloxacin in serum, attaining remarkable detection limits of 1.7 mM and 0.10 μM, respectively. To assess the practical applicability of the system, we tested its performance in sensing ofloxacin in serum and urine, with recovery percentages ranging from 89.2 % to 101.3 % and 83.53 %–101.0 %, respectively, with all relative standard deviations (RSD) below 3.9 %, confirming the reliability and accuracy of the approach for biological sample analysis. Additionally, the y-CDs exhibited outstanding biocompatibility and were successfully employed for cellular imaging in SiHa cells, underscoring their potential in bioimaging applications. This study introduces a versatile chiral nano platform with promising uses in biomedical sensing, environmental monitoring, and diagnostic imaging.
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Bioresources and Bioprocessing
·
2021
·Vol. 8
·期 1
·1-17
·
IF 4.578
Wang Jianfei,Guo Huanyu,Huang Jiaqi,Jiang Shaoming,Hou Shibo,Chen Xingyu,Lv Hujie,Bi Xudong,Hou Maolin,Lin Hebei,Lu Yuming,Qiao Jinyue,Yang Ruiyi,Liu Shijie
Under the optimal conditions of immobilization and fermentation, the highest LA yield of 0.966 ± 0.006 g/g fructose and production rate of 2.426 ± 0.018 g/(L × h) with an error of -0.5% and -0.2% to the predicted results were obtained from batch fermentation by the CS film-coated SA-PVA immobilized L. pentosus cells. The LA yield and production rate of these immobilized cells were 2.7% and 10.1% higher than that of normal SA-PVA immobilized cells respectively, and they were 5.7% and 48.4% higher than that of free cells, respectively. The effect of temperature on different types of immobilized cells and free cells was significantly different, but the effect of pH on different types of cells was not much different. The kinetic models could effectively describe the different fermentation performances of three types of cells. The immobilized cells have excellent reusability to conduct 9 runs of repeated batch fermentation.
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Water Supply
·
2021
·Vol. 21
·期 8
·4429-4441
·
IF 1.033
Sun Xuecheng,Ali Meesam,Cui Changzheng,Lyu Shuguang
The simultaneous oxidation performance of benzene, toluene, ethylbenzene, and xylene (BTEX) by nanoscale calcium peroxide particles (nCaO 2 ) activated with ferric ions (Fe(III)) and the mechanism of the enhancement of BTEX degradation by L-cysteine (L-cys) were investigated. The batch experimental results showed that the nCaO 2 /Fe(III)/L-cys process was effective in the destruction of BTEX in both ultrapure water and actual groundwater. A proper amount of L-cys could enhance BTEX degradation due to the promotion of Fe(II)/Fe(III) redox cycles by the participation of L-cys, but an excessive presence of L-cys would cause inhibition. Adding 1.0 mM L-cys to the nCaO 2 /Fe(III) system, the concentration of Fe(II) increased to 1.15 mM instantly. Simultaneously, the yield of HO• produced by the 1.0 mM L-cys-containing system was 0.066 mM at 180 min reaction, higher than that without L-cys (0.049 mM). When excess L-cys (5.0 mM) was added to the system, the amount of Fe(II) increased to 3.73 mM because excessive L-cys caused a large amount of Fe(III) in the system to be reduced. However, the yield of HO• decreased to 0.043 mM since excessive Fe(II) could conversely scavenge HO• to produce Fe(III) again. EPR tests and quenching results indicated that HO• was the dominant reactive species in the nCaO 2 /Fe(III)/L-cys system. For the removal of BTEX, the optimal molar ratio of nCaO 2 /Fe(III)/L-cys was 10.5/20/1 based on calculation by response surface methodology (RSM). Finally, the BTEX destruction pathway was proposed according to the detected intermediates by liquid chromatography-mass spectrometry (LC-MS). nCaO 2 /Fe(III) system with L-cysteine (L-cys) was applied to remediate mixed contaminants. A suitable amount of L-cys promoted redox cycling (Fe(III) → Fe(II)). The mechanism of enhancing or inhibiting hydroxyl radical generation was investigated. Proposed BTEX destruction pathway in the nCaO 2 /Fe(III)/L-cys system. nCaO 2 /Fe(III)/L-cys system was recommended for BTEX-contaminated actual groundwater remediation.
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Water Supply
·
2021
·Vol. 22
·期 1
·555-573
·
IF 1.033
Sun Xuecheng,Cui Changzheng,Yang Chaoxiang,Lyu Shuguang
Benzene, toluene, ethylbenzene and xylene (BTEX) possess a negative impact on the environment and human beings due to their highly toxic and carcinogenic properties. In this study, persulfate (PS) activated by nano zero-valent iron (nZVI) coupled with chelated L-cysteine (L-cys) process was investigated for BTEX degradation in contaminated groundwater. BTEX degradation had a significant acceleration and improvement with the removal from 62.7 to 100% along with the increasing dosage of L-cys from 0.12 to 0.27 M in 24 h. Further, the compact nZVI catalytic cylinder and nZVI encapsulated L-cys catalytic cylinder were successfully manufactured by encapsulating nZVI, and nZVI and L-cys together with additives of cement, river sand, stearic acid (SA) and zeolite. The SEM image, XRD patterns and FTIR spectra showed that the manufactured catalytic cylinder had a porous structure and encapsulated nZVI and L-cys successfully. Six successive cycles of BTEX degradation were completed and the degradation rate decreased gradually in each cycle. The catalytic activity of nZVI encapsulated L-cys catalytic cylinder was superior to nZVI catalytic cylinder in each cycle. The electron paramagnetic resonance (EPR) results indicated that HO• was the dominant active species in the BTEX degradation process. Benzoic acid (BA) scavenge experiments showed that L-cys could increase the yield of HO• in the PS/nZVI system. The HO• yields of PS/nZVI encapsulated L-cys catalytic cylinder system were 3.2 to 4.8 fold higher than those of the PS/nZVI catalytic cylinder system. The possible mechanisms of PS activation by nZVI encapsulated L-cys catalytic cylinder were supposed. Homogeneous Fenton reaction and heterogeneous catalysis on the nZVI surface are two co-existence mechanisms in the PS/nZVI encapsulated L-cys catalytic cylinder system. The findings of this study provide new insights into the mechanism of nZVI encapsulated L-cys catalytic cylinder activating PS, showing its potential applications for the remediation of groundwater. PS/nZVI/L-cys system was applied to remediate BTEX contaminants. The compact nZVI encapsulated L-cys catalytic cylinder was successfully manufactured. Six successive cycles of BTEX degradation were completed. Two co-existence mechanisms in the PS/nZVI encapsulated L-cys catalytic cylinder system were investigated. PS/nZVI catalytic cylinder systems were recommended for BTEX-contaminated groundwater remediation.
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ACS Sustainable Chemistry & Engineering
·
2026
·Vol. 14
·期 30
·13802-13811
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IF 7.6
Xu Xinyue,Xu Ying,Shi Siwen,Wen Jialong,Yuan Tongqi
The development and functional utilization of light-colored lignin are largely hindered by uncontrolled condensation reactions during extraction, which reduces lignin reactivity and compromises its functional performance. Herein, a formic acid/L-Cysteine (FA/L-Cys) pretreatment system was developed to fractionate functional light-colored lignin from bamboo. Under mild conditions (100 ° C, 1 h), this system achieved 95.3% delignification and 71.3% lignin recovery, affording pale beige lignin with a lightness value of 80.9. 2D-HSQC NMR analysis revealed that L-Cys suppressed lignin condensation by nucleophilically trapping reactive intermediates. When incorporated into a sun protection factor (SPF) 15 sunscreen formulation at 5 wt %, the optimized lignin (L-Cys-0.3) increased the SPF to 71.7. Moreover, L-Cys-0.3-derived lignin nanoparticles efficiently stabilized Pickering emulsions and exhibited a 12-fold enhancement in UV absorption capacity compared with their LNPs aqueous dispersion counterparts. This work highlights condensation-controlled fractionation as an effective strategy for producing light-colored lignin with potential applications in sustainable sunscreens and functional Pickering emulsion systems.
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INORGANIC CHEMISTRY COMMUNICATIONS
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2026
·
IF 5.9
Mei Wang,Yingkuan Sun,Wei Shang,Yaqian Liu,Liying Jiang
Herein, a cost-effective and user-friendly electrochemical sensor was fabricated via a straightforward surface functionalization strategy using L-cysteine-functionalized NiFe 2 O 4 @NiCoFe LDH/MLG nanocomposites, specifically designed for on-site screening of Pb 2+ in Class III-V surface waters. The fabrication process involved the in-situ one-step hydrothermal growth of NiFe 2 O 4 @ternary NiCoFe layered double hydroxide (LDH) nanomaterials on a multilayer graphene (MLG) scaffold, followed by functionalization with L-cysteine (L-Cys) to yield the Cys/NiFe 2 O 4 @NiCoFe LDH/MLG/SPE sensor. Benefiting from the excellent electrical conductivity of MLG, the synergistic enhancement effect of the ternary LDH, and the multiple coordination active sites of L-Cys, the as-prepared sensor exhibits reliable sensing performance for Pb 2+ detection. Under optimal conditions, the sensor achieved a linear dynamic range of 0.1–3 mg/L and a limit of detection (LOD) of 30 μg/L, which is sufficient to discriminate Pb 2+ concentrations exceeding the regulatory threshold for Class III surface water (50 μg/L, GB 3838–2002). The sensor also demonstrated excellent selectivity against common coexisting ions (Co 2+ , Fe 3+ , Cu 2+ , and Zn 2+ ) and satisfactory repeatability (RSD = 4.42%). In real water sample analysis, recoveries ranged from 94.1% to 113%, confirming its practical applicability. Overall, the Cys/NiFe 2 O 4 @NiCoFe LDH/MLG/SPE composite electrode offers a cost-effective, robust, and operationally simple analytical platform, showing great promise for rapid on-site risk-level screening of Pb 2+ contamination in heavily impacted surface waters, particularly in resource-limited regions.
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Gels
·
2026
·Vol. 12
·期 8
·709
·
IF 6.4
Xiuyun Guo,Jinsheng Yang,Chao Fu,Jiangpeng Yao,Zhikun Yang,Xiangren Meng
Reducing sodium in meat products is nutritionally desirable, but salt reduction often weakens myofibrillar protein gelation and reduces texture quality and water retention. This study investigated the effects of L-lysine (Lys) and konjac glucomannan (KGM) on the physicochemical properties, gel characteristics, and structural changes of beef myofibrillar protein (MP) gels under low-salt conditions. The results indicated that reducing NaCl from 0.6 to 0.2 M decreased water-holding capacity (WHC), increased cooking loss, and produced a loose gel network. Compared with the 0.2 M NaCl group, the combined Lys-KGM treatment increased WHC from 28.53% to 56.70% and reduced cooking loss to 22.19% (p< 0.05). Texture analysis showed that Lys-KGM increased hardness and springiness by 69.40% and 80.43%, respectively (p< 0.05). LF-NMR indicated a higher proportion of immobilized water and reduced water mobility in the combined treatment. Lys increased reactive sulfhydryl content and surface hydrophobicity, whereas KGM reduced surface hydrophobicity and enhanced water immobilization. Lys-KGM slightly but significantly decreased α-helix and increased β-sheet contents (p< 0.05), accompanied by changes in the relative contributions of intermolecular forces and a more continuous gel network. Molecular docking and molecular dynamics simulations provided supporting evidence for different interaction patterns between myosin and Lys/KGM. These results suggested that Lys and KGM might help maintain the quality of low-salt meat protein gels and provide a formulation basis for reduced-sodium meat products.
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Food Chemistry-X
·
2026
·
IF 9.2
Ying Liu,Linlin Yu,Shuxiang Yan,Xiaowei Fang,Tenggao Zhu,Huanwen Chen,Dong Wu
Metabolic changes during the ripening of wolfberry ( Lycium barbarum L.) fruits reflect their quality and pharmacological properties. To decipher the metabolic evolution underlying this physiological process, a systematic analysis of wolfberry fruits at different ripening stages (7–35 days after anthesis) was conducted by theta-nano-extractive electrospray ionization mass spectrometry. Multivariate analysis effectively discriminated the various stages and identified proline, γ-aminobutyric acid, arginine, and N 1 -caffeoyl- N 10 -dihydrocaffeoyl spermidine as potential differential metabolites. Polyamine and flavonoid metabolism were significantly activated during the early ripening stages, leading to the rapid accumulation of antioxidant components such as spermidine, rutin, and chlorogenic acid. In the late ripening stages, amino acid metabolism was markedly enhanced, resulting in a substantial increase in betaine, serine, and threonine. This study elucidates the key stages and metabolic foundation of quality formation in wolfberry fruits at the metabolomic level, providing a theoretical basis for determining the optimal harvest time and regulating nutritional quality.
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Journal of Pharmaceutical Analysis
·
2026
·
IF 11.2
Man Zhang,Shanshan Zhai,He Gao,Tong Sun,Kaixin Liu,Wenshuang Wang,Yuanyuan Hou,Gang Bai
Acute pulmonary inflammation, a major cause of morbidity and mortality, involves complex immune responses and increased vascular permeability. Conventional imaging techniques for assessing pneumonia and monitoring vascular leakage have limitations in spatial resolution and practicality. Rhodamine B-conjugated poly-L-lysine (RhB-PLL) self-assembled nanoparticles (NPs) were synthesized for targeting mitochondria and sensing vascular permeability. Dynamic spatiotemporal imaging analysis stratifies the progression of acute pneumonia into four distinct stages. Six phosphatidylcholine metabolites, exhibiting stage-dependent variations, were proposed as potential biomarkers to support RhB-PLL detection. Subsequently, Fuzheng Jiedu decoction (FZJD) was used as a model to validate the rationale of syndrome-specific treatment for pneumonia. Three simplified prescriptions derived from FZJD showed unique advantages at different stages. Xiaochaihu decoction (XCH) relieved inflammation and oxidative stress in mild to moderate cases; Sanren decoction (SR) reduced vascular leakage and prevented pulmonary edema in moderate to severe cases; Maxingshigan decoction (MXSG) improved mitochondrial function, prevented microcirculatory disorders in severe to critical cases. The combined prescription, FZJD, mitigated disease progression in mild to moderate cases and demonstrated the benefits of overall intervention. This conclusion was further substantiated by metabolomics cluster analysis. Real-time RhB-PLL multimodal imaging enables precise visualization and detection of inflammation progress, facilitates therapy assessment, and enhances the understanding of multicomponent drug combinations.
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Frontiers in Pharmacology
·
2026
·
IF 5.4
Jia-Lu Wang,Xing-Xing Liu,Xin-Yu Li,Chuan-Gui Wang,Hong-Yu Wang,Jing-Han Jia,Yu-Yue Luo,Xin-Hua Song,Chao Wang,Wen-Long Sun
Introduction Alcohol-associated liver disease (ALD) represents a growing global health burden, for which safe and accessible therapeutic options remain limited. As a widely used medicinal and food plant, Piper nigrum L. contains piperine as its core hepatoprotective alkaloid. However, conventional organic solvent extraction poses environmental risks, leaves solvent residues, and causes loss of synergistic endogenous bioactives. As sustainable green extraction media, deep eutectic solvents (DESs) show great potential for phytochemical enrichment, yet their impacts on the hepatoprotective potency and gut microbiota-regulatory activity of black pepper extracts remain unclear. Methods A choline chloride-1,4-butanediol-based ultrasonic-assisted DES extraction system was optimized via single-factor experiments and Box-Behnken design. We systematically compared the phytochemical profiles, in vitro antioxidant activities, and in vivo hepatoprotective efficacies of DES extracts versus conventional 60% ethanol extracts using a mouse model of acute ethanol-induced liver injury, and employed 16S rRNA gene sequencing to analyze gut microbiota composition. Results Under the optimized conditions (extraction time of 60 min, water content of 30%, solid-to-liquid ratio of 1:28 g/mL, molar ratio of choline chloride to 1,4-butanediol of 1:3, ultrasonic power of 400 W, and temperature of 40 °C), the DES process achieved a piperine yield of 44.40 ± 0.84 mg/g. In the DPPH radical scavenging assay, DES extract exhibited better in vitro antioxidant activity than the ethanol extract. In mice with acute ethanol-induced liver injury, DES extract reduced hepatic lipid accumulation, oxidative stress, and hepatocellular injury. 16S rRNA sequencing revealed that DES extract treatment was correlated with altered gut microbial composition under ethanol stimulation, including decreased relative abundance of Muribaculaceae and Clostridia UCG-014, and increased relative abundance of Lactobacillus murinus. Discussion Collectively, these findings indicate that DES-derived black pepper extract is associated with favorable hepatoprotective phenotypes in the acute ethanol-induced liver injury model, alongside distinct correlational changes in gut microbiota composition. The study provides technical support for green high-value processing of plant-based spice resources, and scientific insights for developing microbiota-targeted plant-derived functional foods.
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危险品化学品经营许可证（带存储）