WATER AIR AND SOIL POLLUTION
·
2021
·Vol. 232
·期 9
·1-14
·
IF 2.52
Han Rui,Li Kexin,We Chenzheng,He Tao,Liu Jingru,Zhang Renduo,Wan Li,Wu Yinghai
Titanium dioxide nanoparticles (TiO 2 NPs) can potentially impact constructed wetlands (CWs). The aim of this study was to investigate the rapid impacts on nitrogen removal performance and on the microbial community after short-term exposure to TiO 2 NPs in CWs, which were determined via 16S rDNA amplicon sequencing and Biolog-Eco technologies. The results showed that removal efficiencies declined by 23.9%, 32.2%, and 38.8% for ammonia nitrogen (NH 4 + -N) and by 26.5%, 43.4%, and 71.4% for nitrate nitrogen at TiO 2 NP concentrations of 50, 100, and 150 mg/L, respectively. Bacterial damage and TiO 2 NP agglomeration on the packing increased as the TiO 2 NP concentration increased. Microbes showed different tolerances against TiO 2 NPs, and members, such as the bacteria removing organic pollutants and NH 4 + -N, were susceptible to TiO 2 NPs. The order of microbial utilisation of the six carbon sources was determined, and the utilisation ratios declined as TiO 2 NP concentration increased. The concentration of 50 mg/L TiO 2 NPs impaired the functions of nitrifiers and denitrifiers in CWs after short-term exposure, and higher concentrations (100 mg/L and 200 mg/L) had no additional negative impact on the packing compared with the concentration of 50 mg/L.
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EUROPEAN JOURNAL OF NUTRITION
·
2019
·Vol. 59
·期 6
·2709-2728
·
IF 4.449
Wang Tao,Yan Hong,Lu Yingying,Li Xin,Wang Xin,Shan Yuanyuan,Yi Yanglei,Liu Bianfang,Zhou Yuan,Lü Xin
Purpose The objective of the study was to evaluate the anti-obesity effect of Lactobacillus rhamnosus LS-8 and Lactobacillus crustorum MN047, and illustrate the potential functional mechanism about the alleviation of high fat and high fructose diet (HFFD) induced obesity and related metabolic abnormalities. Methods C57BL/6J mice were subjected to a standard or HFFD with or without supplementation of L. rhamnosus LS-8 and L. crustorum MN047 for 10 weeks. Obesity related metabolic indices including glucose tolerance, insulin resistance, serum lipid, liver function, hormones and inflammatory cytokines were assessed by standard protocols. For the monitoring of inflammatory response and lipid metabolism, transcriptional levels were profiled in liver and/or adipose tissues. Furthermore, gut microbiota composition analyses in the fecal samples were performed using 16S rRNA gene sequencing, and gut microbial metabolites, including lipopolysaccharide (LPS) and short-chain fatty acids (SCFAs), were also tested for the assessment of the relationship between gut microbiota variation and inflammatory response. Results Administration with L. rhamnosus LS-8 and L. crustorum MN047 significantly mitigated body weight gain and insulin resistance, and inflammatory response (TNF-α, IL-1β and IL-6 levels in serum and corresponding mRNA levels in adipose tissues) was significantly inhibited in these two strains-treated mice. Moreover, L. rhamnosus LS-8 and L. crustorum MN047 could partially normalized mRNA expression levels involved in lipid metabolism including Pparγ , Srebp - 1c , CD36 , Fabp2 and FAS . In addition, these two strains manipulated gut microbiota by decreasing the abundance of Bacteroides and Desulfovibrio and increasing that of Lactobacillus and Bifidobacterium , which in turn raised the levels of feces SCFAs and lowered the levels of circulating LPS. Conclusion These results indicated that L. rhamnosus LS-8 and L. crustorum MN047 supplementation possessed the anti-obesity effect on the HFFD fed mice by alleviating inflammatory response and regulating gut microbiota, which further suggested that these two probiotics can be considered as an alternative dietary supplement in combination with the preventive and therapeutic strategies against obesity and related complications. Graphic abstract
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CURRENT MICROBIOLOGY
·
2014
·Vol. 70
·期 1
·67-74
·
IF 1.359
Liu Jing,Pu Yi-Yi,Xie Qian,Wang Jia-Kun,Liu Jian-Xin
Pectin is a non-fiber carbohydrate (NFC) that exists in forages, but it is not clear how pectin exerts its effect on populations of either known microbial species or uncultured ruminal bacteria. PCR-denaturing gradient gel electrophoresis (PCR-DGGE) and real-time PCR analysis were used in the present study to investigate the effects of pectin on microbial communities in an in vitro rumen fermentation system. The fermentations were conducted using forage (corn stover or alfalfa), an NFC source (pectin or corn starch), or their combination as the substrates. Addition of pectin increased acetate ( P < 0.05), whereas inclusion of starch increased butyrate production ( P < 0.05). The pectate lyase activity was higher with alfalfa than with corn straw, or with pectin than with corn starch ( P < 0.05), while the amylase activity was higher in corn starch-included treatments than the others ( P < 0.05). The cluster analysis of the bacterial 16S rRNA gene showed that the DGGE banding patterns differed significantly between the treatments and led to the identification of three groups that were highly associated with the NFC sources. The specific bands associated with pectin-rich treatments were identified to be dominated by members of the Treponema genus. The growth of the Treponema genus was remarkably supported by the inclusion of pectin, highlighting their specific ability to degrade pectin. The results from the present study expand our knowledge of the microbial populations associated with pectin digestion, which may not only facilitate future research on utilization of pectin in feeds, but also improve our understanding of pectin digestion with respect to the rumen micro-ecosystem.
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PLANT CELL TISSUE AND ORGAN CULTURE
·
2020
·Vol. 143
·期 3
·665-679
·
IF 2.196
Liu Qun,Zhou Wei,Ruan Qinyan,Cheng Haitao,Liu Tingyao,Wang Leran,Yuan Yuan,Li Li,Wu Jie,Jiang Jihong,Ning Wei,Kai Guoyin
WRKY transcription factors play a critical role in metabolism and stress responses in plants, but few WRKYs have been reported in Taraxacum antungense . Here, a multiple stress-inducible gene, TaWRKY14 , was isolated from T. antungense . TaWRKY14 was localized to the nuclei, and phylogenetic analysis indicated that TaWRKY14 shared the highest identity to WRKY40 in Salvia miltiorrhiza . TaWRKY14 was highly expressed in roots, and up-regulated by salt and drought stress, salicylic acid treatment and powdery mildew. TaWRKY14 -overexpressing transgenic lines had higher chlorogenic acid concentration and high expression of TaPAL1 . The yeast one-hybrid assay proved that TaWRKY14 bond to the W-box of proTaPAL1 . Additionally, field experiments showed that TaWRKY14 -overexpressing T. antungense lines have higher powdery mildew resistance than the wild-type. Our results demonstrated that TaWRKY14 can regulate CGA biosynthesis and play an important role in resistance to powdery mildew in T. antungense . TaWRKY14 transgenic T. antungense can be used for further evaluation as a new germplasm resource.
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2014
·Vol. 71
·期 2
·380-384
·
IF 1.547
Li Binjie,Zhang Yongna,Cao Liuqin,Wu Yonghui,Zhao Yanbao
Silica (SiO 2 ) nanospheres (NSs) with immobilized metal ligands have been prepared for the affinity separation of proteins. First, SiO 2 NSs were prepared by controlled hydrolysis of tetraethoxysilane in a basic aqueous-ethanol solution. Then through reaction of iminodiacetic acid (IDA) with 3-glycidoxypropyltrimethoxysilane and immobilization of them onto the surfaces of above SiO 2 NSs, novel affinity adsorbents with IDA chelating groups were obtained. After chelating Ni 2+ ions, the SiO 2 –IDA–Ni 2+ NSs were applied to separate his-tagged proteins directly from the mixture of lysed cells. The SiO 2 –IDA–Ni 2+ NSs present negligible nonspecific protein adsorption and high protein binding ability (28.3 mg/g).
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JOURNAL OF NANOBIOTECHNOLOGY
·
2021
·Vol. 19
·期 1
·1-17
·
IF 10.435
Liao Haoran,Yu Han-Ping,Song Wei,Zhang Guangcheng,Lu Bingqiang,Zhu Ying-Jie,Yu Weilin,He Yaohua
Background Rotator cuff tear (RCT) is a common problem of the musculoskeletal system. With the advantage of promoting bone formation, calcium phosphate materials have been widely used to augment tendon-bone healing. However, only enhancing bone regeneration may be not enough for improving tendon–bone healing. Angiogenesis is another fundamental factor required for tendon–bone healing. Therefore, it’s necessary to develop a convenient and reliable method to promote osteogenesis and angiogenesis simultaneously, thereby effectively promoting tendon–bone healing. Methods The amorphous calcium phosphate (ACP) nanoparticles with dual biological activities of osteogenesis and angiogenesis were prepared by a simple low-temperature aqueous solution method using adenosine triphosphate (ATP) as an organic phosphorus source. The activities of osteogenesis and angiogenesis and the effect on the tendon–bone healing of ACP nanoparticles were tested in vitro and in a rat model of acute RCT. Results The ACP nanoparticles with a diameter of tens of nanometers were rich in bioactive adenosine. In vitro, we confirmed that ACP nanoparticles could enhance osteogenesis and angiogenesis. In vivo, radiological and histological evaluations demonstrated that ACP nanoparticles could enhance bone and blood vessels formation at the tendon–bone junction. Biomechanical testing showed that ACP nanoparticles improved the biomechanical strength of the tendon–bone junction and ultimately promoted tendon–bone healing of rotator cuff. Conclusions We successfully confirmed that ACP nanoparticles could promote tendon–bone healing. ACP nanoparticles are a promising biological nanomaterial in augmenting tendon–bone healing. Graphic abstract
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Cell Death & Disease
·
2018
·Vol. 9
·期 9
·1-11
·
IF 5.638
Wang Yunchuan,Zheng Jianping,Han Yue,Zhang Yijie,Su Linlin,Hu Dahai,Fu Xiaobing
Junctional adhesion molecule-A (JAM-A) belongs to the immunoglobulin superfamily, it predominantly exists at the tight junctions of epithelial and endothelial cells. JAM-A is known to regulate leukocyte trans-endothelial migration, however, how it affects the proliferation and migration of keratinocytes, the two essential steps during wound healing, has less been explored. In this study, we showed that JAM-A was significantly expressed in normal skin epidermis. RNAi-mediated JAM-A knockdown remarkably promoted the proliferation and migration of keratinocytes. We also found that loss of JAM-A increased the protein levels of p -FAK, p -Erk1/2, and p -JNK; however, FAK inhibitor PF-562271 restrained the expression of p -FAK and p -Erk1/2 elevated by JAM-A RNAi, but not p -JNK, and also slowed down keratinocyte proliferation and migration. Finally, in a rat wound model we showed that absence of JAM-A significantly promoted the wound healing process, while the use of PF-562271 or Erk1/2 inhibitor PD98059 repressed those effects. These data collectively demonstrate that suppressing JAM-A expression could promote the proliferation and migration of keratinocytes and accelerate the healing process of rat skin wounds, potentially via FAK/Erk pathway, indicating that JAM-A might serve as a potential therapeutic target for the treatment of chronic refractory wounds.
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Food Analytical Methods
·
2026
·Vol. 19
·期 12
·407
·
IF 3
Chen Qiming,Zhang Hua,Duan Ke,Gao Qinghua,Frango Alberto,Liu Zhanmin
CRISPR/Cas9 genome editing is increasingly used in crop improvement, creating a need for practical tools to screen plant-derived materials for retained editing components. We developed a probe-free fluorescent loop-mediated isothermal amplification (LAMP) assay that uses thioflavin T (ThT) to detect the Cas9 coding sequence in genome-edited tomato materials. Under the tested conditions, the fluorescence calibration yielded an operational plasmid-based threshold estimate of 4.34 copies/μL; this value is not a validated limit of detection, and additional low-copy replicates are required. Cas9-positive tomato DNA was distinguished from wild-type tomato, four tested non-target plant DNA samples, and a no-template control, and four independent Cas9-positive tomato lines generated positive signals. Analysis of G-rich amplicon motifs and sequence-matched oligonucleotides suggested sequence-dependent contributions to ThT fluorescence, although G-quadruplex formation in LAMP products was not directly confirmed. The assay uses the same Cas9 target and primer framework as a previous HNB-based colorimetric method; its contribution is an instrument-recorded, probe-free fluorescence readout rather than broader target coverage or demonstrated superiority over existing fluorescent chemistries. The method is therefore a proof-of-concept approach for preliminary screening. Validation in processed foods, formal threshold development, low-copy replication, and closed-tube implementation remain necessary before routine use.
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Environmental Sciences Europe
·
2020
·Vol. 32
·期 1
·1-14
·
IF 5.394
Wu Lingling,Chen Ling,Zhu Lingjiao,Chen Xiaoping,Li Qian
Sediments function as a secondary and significant source of tributyltin (TBT) and triphenyltin (TPT) in aquatic ecosystems and may pose a potential threat on benthic organisms and human health. The subchronic toxicity of sediment-associated TBT or TPT to snails Bellamya aeruginosa at environmentally relevant concentrations was investigated in this study. Multiple endpoints at the biochemical [ethoxyresorufin-O-deethylase (EROD), superoxide dismutase (SOD), catalase (CAT), protein carbonyl content (PCC) and lipid peroxidation (LPO)] and transcriptomic levels were examined.TBT or TPT in sediment could induce antioxidant enzymes’ activities and result in oxidative damage in the hepatopancreas of B. aeruginosa after 28-day exposure. A transcriptomic profile of B. aeruginosa exposed to TBT and TPT was reported. CYP genes and EROD activity were sensible and reliable biomarkers for toxicity assessment of TBT or TPT in sediments. Comparative pathway analysis revealed the alteration of steroid hormone biosynthesis and retinol metabolism in B. aeruginosa after 90-day exposure to sediment-associated TBT at the concentration of 2000 ng/g dw, which might affect both reproduction and lipogenesis functions. The ubiquitin proteasome system and immune system might be the toxicity target in B. aeruginosa after exposure to sediment-associated TPT for 90 days.The results offered new mechanisms underlying the toxicity of sediment-associated tributyltin and triphenyltin.
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AAPS PHARMSCITECH
·
2021
·Vol. 22
·期 1
·1-16
·
IF 3.246
Sun Congyong,Li Wenjing,Zhang Huiyun,Adu-Frimpong Michael,Ma Ping,Zhu Yuan,Deng Wenwen,Yu Jiangnan,Xu Ximing
This study aimed to develop a self-microemulsifying drug delivery system (SMEDDS) to enhance the solubility, oral bioavailability, and hypolipidemic effects of syringic acid (SA), a bioactive and poorly-soluble polyphenol. Based on the response surface methodology-central composite design (RSM-CCD), an optimum formulation of SA-SMEDDS, consisting of ethyl oleate (oil, 12.30%), Cremophor-EL (surfactant, 66.25%), 1,2-propanediol (cosurfactant, 21.44%), and drug loading (50 mg/g), was obtained. The droplets of SA-SMEDDS were nanosized (16.38 ± 0.12 nm), spherically shaped, and homogeneously distributed (PDI = 0.058 ± 0.013) nanoparticles with high encapsulation efficiency (98.04 ± 1.39%) and stability. In vitro release study demonstrated a prolonged and controlled release of SA from SMEDDS. In vitro cell studies signified that SA-SMEDDS droplets substantially promoted cellular internalization. In comparison with the SA suspension, SA-SMEDDS showed significant prolonged T max , t 1/2 , and MRT after oral administration. Also, SA-SMEDDS exhibited a delayed in vivo elimination, increased bioavailability (2.1-fold), and enhanced liver accumulation. Furthermore, SA-SMEDDS demonstrated significant improvement in alleviating serum lipid profiles and hepatic steatosis in high-fat diet-induced hyperlipidemia in mice. Collectively, SMEDDS demonstrated potential as a nanosystem for the oral delivery of SA with enhanced bioavailability and hypolipidemic effects.
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Microbial Cell Factories
·
2021
·Vol. 20
·期 1
·1-11
·
IF 5.328
Wan Yupeng,Liu Hongchen,Xian Mo,Huang Wei
Background 1-Hydroxyphenazine (1-OH-PHZ) is a phenazine microbial metabolite with broad-spectrum antibacterial activities against a lot of plant pathogens. However, its use is hampered by the low yield all along. Metabolic engineering of microorganisms is an increasingly powerful method for the production of valuable organisms at high levels. Pseudomonas chlororaphis is recognized as a safe and effective plant rhizosphere growth-promoting bacterium, and faster growth rate using glycerol or glucose as a renewable carbon source. Therefore, Pseudomonas chlororaphis is particularly suitable as the chassis cell for the modification and engineering of phenazines. Results In this study, enzyme PhzS (monooxygenase) was heterologously expressed in a phenazine-1-carboxylic acid (PCA) generating strain Pseudomonas chlororaphis H18, and 1-hydroxyphenazine was isolated, characterized in the genetically modified strain. Next, the yield of 1-hydroxyphenazine was systematically engineered by the strategies including (1) semi-rational design remodeling of crucial protein PhzS, (2) blocking intermediate PCA consumption branch pathway, (3) enhancing the precursor pool, (4) engineering regulatory genes, etc. Finally, the titer of 1-hydroxyphenazine reached 3.6 g/L in 5 L fermenter in 54 h. Conclusions The 1-OH-PHZ production of Pseudomonas chlororaphis H18 was greatly improved through systematically engineering strategies, which is the highest, reported to date. This work provides a promising platform for 1-hydroxyphenazine engineering and production. Graphical Abstract
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MONATSHEFTE FUR CHEMIE
·
2020
·Vol. 151
·期 1
·99-106
·
IF 1.349
Li Nutao,Chen Feng,Wang Guanghui,Zeng Qingle
Abstract A ligand-free copper-catalyzed C–P cross-coupling reaction of arylmethyl quaternary ammonium salts and diarylphosphine oxides in air is developed. Arylmethyl quaternary ammonium salts with various functional groups and a variety of dialkyl- and diarylphosphine oxides afford C–P cross-coupling products with good yields. This protocol requires no inert atmosphere, no ligand, and simple operation steps. Graphic abstract
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危险品化学品经营许可证（带存储）