ACTA BIOCHIMICA POLONICA
·
2021
·Vol. 68
·期 4
·765-774
·
IF 2.149
Shaoyi Li,Yong Li,Bibo Tan,Zhaojie An
Krüppel-like factor 1 (KLF1) is a transcription factor that exhibits promoting effect in cervical cancer, but its correlation with gastric cancer (GC) has not been reported yet. In this study, we explored the role and potential mechanism of KLF1 in GC progression by using a series of experimental methods including RT-qPCR, Western blot, CCK-8 assay, EdU staining, and cell cycle analysis. KLF1 was found to be elevated in GC tissues (n=415) compared with the normal tissues by applying UALCAN to analyze datasets from The Cancer Genome Atlas (TCGA). The upregulation of KLF1 was also validated in GC cell lines. Functional studies proved that RNA interference-mediated silencing of KLF1 inhibited GC cell growth, as evidenced by the decreased cell viability, DNA synthesis, and arrested cell cycle in G1 phase. Moreover, KLF1 knockdown exerted the inhibitory effects on cell migration and invasion as well as the epithelial-mesenchymal transition (EMT) in GC cells. Conversely, overexpression of KLF1 had the opposite effects on GC progression. Furthermore, we proved that the activation of Wnt/β-catenin pathway was markedly inhibited by KLF1 knockdown and promoted by KLF1 overexpression. The blockade of Wnt/β-catenin pathway rescued the effects of KLF1 overexpression. These results suggested that KLF1 promoted the growth, migration, invasion, and EMT process in GC cells, and this promotion was achieved by activating the Wnt/β-catenin pathway. This work will be helpful for searching the potential therapeutic targets for treatment of GC.
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Yuanting Zhu,Guizhou Xu,Xueyao Wang,Xiaowen Ji,Xun Jia,Lin Sun,Xueyuan Gu,Xianchuan Xie
Chlorophenols (CPs) have been listed as priority control pollutants because of their high toxicity and wide range. An In-situ monitoring technique using diffusive gradients in thin films based on porous β-cyclodextrin polymers as binding materials (CDP-DGT), was established to monitor four typical CPs, namely, 4-Chlorophenol (4-CP), 2,4-Dichlorophenol (2,4-DCP), 2,4,5-Trichlorophenol (2,4,5-TCP), 2,4,6-Trichlorophenol (2,4,6-TCP) in water and soils. The performance of CDP-DGT are stable under the conditions of pH 3.5–9.3, ionic strength 0.001–0.500 mol L −1 and dissolved organic matter concentration 0–20 mol L −1 . The adsorption capacities of CDP-DGT for 4-CP, 2,4-DCP, 2,4,5-TCP, 2,4,6-TCP were 57.80 μg cm −2 , 98.82 μg cm −2 , 95.69 μg cm −2 and 98.91 μg cm −2 , respectively. The time-average weighted concentrations of four CPs determined by CDP-DGT at Sanjiangkou wharf (Yangtze river, China) were consistent with the results of grab sampling, indicating the feasibility of CDP-DGT application in actual water. In addition, the distribution of CPs in the red soil of Kunming and paddy soil of Yixing were also studied by CDP-DGT, and the desorption kinetics in the two soils were analyzed with the DIFS model. The higher the soil organic matter content is, the more CPs are distributed in the soil solid phase. CPs in both soils can be partially resupplied to soil solution from the soil solid phase and the higher the partition coefficient for labile CPs is, the stronger the supplement capacity is.
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高影响因子
ACS Applied Polymer Materials
·
2019
·
IF 0
Jinshui Liu,Huijuan Bao,Chenfu Liu,Fangfei Wu,Feng Gao
β-Glucosidase is associated with many diseases, so it is important to be able to accurately determine β-glucosidase activity. Here, we describe a turn-on fluorescence method for sensitively determining β-glucosidase activity. The method involves the formation of water-soluble fluorescent polymer nanoparticles. β-Glucosidase initially hydrolyzes the β-arbutin substrate to release hydroquinone (1,4-dihydroxybenzene). The hydroquinone then forms cross-links between branched polyethylenimine molecules to give fluorescent water-soluble polymer nanoparticles. The polymer nanoparticles were found to fluoresce with excitation and emission peaks at 373 and 510 nm, respectively, and the fluorescence intensity was related to the β-glucosidase activity. The fluorescence intensity could therefore be used to determine the β-glucosidase activity. The lowest β-glucosidase activity that could be determined was 0.4 U L–1, and the dynamic linear range was 1.0–36.0 U L–1. The method was very selective for β-glucosidase activity and was not sensitive to other proteins. The method was successfully used to determine β-glucosidase in spiked samples containing human serum albumin at a concentration of 0.5%, and the recoveries were 95.2%–104.5%.
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CRYSTENGCOMM
·
2020
·Vol. 22
·期 8
·1359-1367
·
IF 3.117
Qinqin Shao,Chao Yang,Xiaoxu Chen,Hong Zhang,Guoying Feng,Shouhuan Zhou
Lanthanide doped hexagonal phase (β) rare earth fluoride (NaREF4) nanocrystals have considerable potential in laser and, especially, biological applications. However, their large size and extreme synthetic conditions have become unavoidable obstacles in their path to various applications. Herein, a core-mediated hetero-shell growth method was adopted to synthesize α-NaGdF4@β-NaLuF4:Nd3+ nanocrystals, in which cubic phase (α) NaGdF4 was used as the core to induce the subsequent growth of a β-NaLuF4:Nd3+ shell via a two-step solvothermal reaction. The resulting α-NaGdF4@β-NaLuF4:Nd3+ core–shell nanocrystals were characterized by structural analysis, photoluminescence spectroscopy and decay lifetime. Besides, a persuasive lattice-disordered heterointerface-induced mechanism was proposed to understand the core-mediated hetero-shell growth. Meanwhile, the influence of the heterointerface on the dynamic growth of the shell nanocrystals was also investigated in detail. Most importantly, the near-infrared luminescence intensity at 1.06 μm was enhanced by over two times via coating the lattice-matched homogeneous NaLuF4 shell on the surface of α-NaGdF4@β-NaLuF4:Nd3+ nanocrystals. The high-performance α-NaGdF4@β-NaLuF4:Nd3+ nanocrystals thus obtained are expected to be used for laser output and depth detection of biological tissues.
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JOURNAL OF AOAC INTERNATIONAL
·
2019
·Vol. 97
·期 1
·121-127
·
IF 1.201
Wang Lili,Deng Jian,Ji Xuezhen,Liu Wanbin,Liang Jun,Yan Xiaona,Chen Dan,Xie Jie
A novel derivative β-cyclodextrin (β-CD) bonded silica gel (β-CD/SiO2) was prepared via carbodiimide activation and used as a chiral stationary phase (CSP). The β-CD/SiO2 CSP was characterizedby X-ray photoelectron spectroscopy and FTIR spectroscopy to prove the successful immobilization of β-CD onto the surface of the silica gel.The thermal behavior of the CSP was studied by differential scanning calorimetry and then used forthe chiral separation of racemic naproxen using HPLC. Several factors affecting the separation efficiency of naproxen enantiomers were investigatedsystematically. The following parameters were selected: methanol–0.01 M phosphate buffer solution (85 + 15, v/v, pH 3.5) was the mobile phase, the flow rate was 1.0 mL/min, and the column temperature was 35°C. Under optimal conditions, the racemic naproxen was efficiently separated with a resolution factor (RS) of 1.70 and a separation factor (α) of 1.25. This method was successfully used for the enantiomeric separation and purity assessment of naproxen in commercial naproxen sustained release tablets.
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Frontiers in Chemistry
·
2019
·
IF 3.782
Wang Xindong,Chen Guanying
Lanthanide-doped upconverting nanoparticles (UCNPs) are promising for applications as wide as biosensing, bioimaging, controlled drug release, and cancer therapy. These applications require surface engineering of as-prepared nanocrystals, commonly coated with hydrophobic ligand of oleic acid, to enable an aqueous dispersion. However, literature-reported approaches often require a long time and/or multiple step treatment, along with several fold upconversion luminescence (UCL) intensity decrease. Here, we describe a strategy allowing oleate-capped UCNPs to become water-soluble and open-modified, with almost undiminished UCL, through ultrasonication of minutes. The prompt phase transfer was enabled by oleate-mediated supramolecular self-assembly of amino modified β-cyclodextrin (amino-β-CD) onto UCNPs surface. We showed that this method is valid for a wide range of UCNPs with quite different sizes (6–400 nm), various dopant types (Er, Tm, and Ho), and hierarchical structures (core, core-shell). Importantly, the amino group of amino-β-CD on the surface of treated UCNPs provide possibilities to introduce entities for biotargeting or functionalization, as exemplified here, a carboxylic-containing near infrared dye (Cy 7.5) that sensitizes UCNPs to enhance their UCL by ~4,820 fold when excited at ~808 nm. The described method has implications for all types of oleate-capped inorganic nanocrystals, facilitating their myriad bioapplications.
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高影响因子
Nanoscale Research Letters
·
2019
·Vol. 14
·期 1
·1-10
·
IF 3.159
Yuan Maohui,Wang Rui,Zhang Chaofan,Yang Zining,Yang Xu,Han Kai,Ye Jingfeng,Wang Hongyan,Xu Xiaojun
The presence of manganese ions (Mn 2+ ) in Yb/Er-co-doped nanomaterials results in suppressing green (545 nm) and enhancing red (650 nm) upconversion (UC) emission, which can achieve single-red-band emission to enable applications in bioimaging and drug delivery. Here, we revisit the tunable multicolor UC emission in a single Mn 2+ -doped β-NaYF 4 :Yb/Er microcrystal which is synthesized by a simple one-pot hydrothermal method. Excited by a 980 nm continuous wave (CW) laser, the color of the single β-NaYF 4 :Yb/Er/Mn microrod can be tuned from green to red as the doping Mn 2+ ions increase from 0 to 30 mol%. Notably, under a relatively high excitation intensity, a newly emerged emission band at 560 nm ( 2 H 9/2 → 4 I 13/2 ) becomes significant and further exceeds the traditional green (545 nm) emission. Therefore, the red-to-green (R/G) emission intensity ratio is subdivided into traditional (650 to 545 nm) and new (650 to 560 nm) R/G ones. As the doped Mn 2+ ions increase, these two R/G ratios are in lockstep with the same tunable trends at low excitation intensity, but the tunable regions become different at high excitation intensity. Moreover, we demonstrate that the energy transfer (ET) between Mn 2+ and Er 3+ contributes to the adjustment of R/G ratio and leads to tunable multicolor of the single microrod. The spectroscopic properties and tunable color from the single microrod can be potentially utilized in color display and micro-optoelectronic devices.
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高影响因子
APPLIED SURFACE SCIENCE
·
2019
·
IF 5.155
Maiyong Zhu,Qi Chen,Jingjing Tang,Wenjing Wei,Songjun Li
The combination of carbon and iron oxide has recently attracted increasing research interest in energy conversion and storage . Here, the hybrid nanostructure with N, S-codoped Fe 3 O 4 supported/embedded on/in N-doped porous carbon (NS-Fe 3 O 4 @N-PC) nanococoon was prepared via direct pyrolysis of core@shell β-FeOOH@Polypyrrole (β-FeOOH@PPy) composite. Benefiting from the synergistic effect of high redox activity of NS-Fe 3 O 4 and excellent electrical conductivity as well as good mechanical stability of N-PC, the obtained NS-Fe 3 O 4 @N-PC exhibited an enhanced specific capacitance of 866 F/g at 1 A/g along with excellent rate capability (383 F/g at 10 A/g) and improved cycling stability (78.2% of initial capacitance retained after 5000 cycles test) while applied as electrode materials of supercapacitor . Significantly, the asymmetric supercapacitor devices assembled using NS-Fe 3 O 4 @N-PC as anodes and commercial carbon nanotubes as cathodes can achieve a large stack energy density of 38.9 Wh/Kg at a stack power density of 700.2 W/Kg along with good stability (only 8.4% decay of initial capacitance after 5000 cycles at 4 A/g). The present work may provide a general approach for fabricating other metal oxide@carbon hybrid nanomaterials for various applications.
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COLORATION TECHNOLOGY
·
2019
·Vol. 135
·期 3
·244-249
·
IF 1.241
Wen Wang,Zhuizhui Fan,Qing Zhu,Mufang Li,Ke Liu,Kelu Yan,Dong Wang
In this paper, highly absorbent poly(vinyl alcohol- co -ethylene) nanofibre membranes modified by β-cyclodextrin were prepared to adsorb dyestuff from water, and 1,2,3,4-butanetetra carboxylic acid was used as a crosslinking agent, which greatly enhanced the adsorption capacity of the modified membranes. Field emission scanning electron microscopy and Fourier Transform–infrared spectroscopy were used to characterise the surface morphology and chemical structures of the membranes. Methylene Blue (MB) was used as the main adsorbed dye. The effect of pH value and concentration of the MB solution were also investigated, and equilibrium adsorption reached 139.2 mg/g when the pH value was 10.0. The adsorption process fitted well with the Langmuir adsorption isotherm model and was in accord with the pseudo-second-order kinetic model. Moreover, the modified membranes proved to have selective adsorption, especially for some cationic dyes other than MB, and had the potential to be recycled multiple times.
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Journal of the Taiwan Institute of Chemical Engineers
·
2019
·
IF 3.834
Pengchao Xie,Yujia Zou,Shan Jiang,Zongping Wang,Jingwen Wang,Li Zhang,Siyang Yue,Xiaonan Feng
Good degradation of β -blocker propranolol (PrP) in the VUV/UV 254 system was achieved in both pure water and realistic water. Both hydroxyl radical (HO • ) and UV 254 photolysis have been evidenced to contribute to PrP degradation in the VUV/UV 254 system. A kinetic model by including both HO • and UV 254 photolysis was successfully applied to predict the observed rate constant ( k obs ) of PrP degradation in the VUV/UV 254 system under different conditions. Elevating initial PrP concentration would reduce the steady-state concentration of HO • , as a result to decreasing the k obs of PrP degradation. While elevating reaction temperature can significantly accelerate PrP degradation. The degradation of PrP in the VUV/UV 254 system was little affected by the variation of reaction pH. HCO 3 − /CO 3 2− , humic acid (HA) and Cl − significantly inhibit PrP degradation in the VUV/UV 254 system. The PrP degradation pathways initiated with hydroxylation and cleavage of side chain were proposed. Although some intermediate products containing higher acute toxicity than PrP were produced in the initial phase of PrP degradation, they could be further oxidized to low toxic products in the system. This study provides that VUV/UV 254 would be a good advanced oxidation process for the treatment of β -blockers in aquatic environment.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2019
·
IF 3.375
Kejing Wu,Chunyan Yang,Yingming Zhu,Junbo Wang,Xueting Wang,Changjun Liu,Yingying Liu,Houfang Lu,Bin Liang,Yongdan Li
Molybdenum carbide catalysts over activated carbon with different crystal phases were synthesized under controllable carburization atmospheres and temperatures for transfer hydrogenation of Kraft lignin in ethanol to aromatic monomers. Single α-MoC1–x and crystal transformation of α-MoC1–x to β-Mo2C are observed under a CH4/H2 mixture and H2 carburization atmospheres, respectively. The mixed crystal phases of α-MoC1–x and β-Mo2C with different crystallinities under a N2 carburization atmosphere exhibit higher activity with a maximum aromatic yield of 0.5163 g/g Kraft lignin when carburized at 700 °C. The dehydrogenation reaction and Kraft lignin hydrogenation are proposed to explain the synergistic effect of β-Mo2C, base, and α-MoC1–x for the base-promoted transfer hydrogenation process in ethanol, which is confirmed by in situ Fourier transform infrared analysis. This highly efficient process is suitable for the conversion of different lignin feedstock.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2020
·Vol. 24
·期 3
·545-555
·
IF 2.646
Pan Yufu,Luo Yanfang,Li Chengcheng,Wang Rongjing,Yuan Yihao,Li Meng,Na Ping
The present study focused on the preparation, characterization, and application of cerium (Ce) and neodymium (Nd) co-doped lead (PbO 2 ) electrode, i.e., Ti/SnO 2 -Sb 2 O 3 /α-PbO 2 /Ce-Nd-β-PbO 2 . The electrochemical activities of the modified electrode were investigated and compared with those of Ce-PbO 2 , Nd-PbO 2 , and pure PbO 2 electrodes. Scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) were used to characterize the surface morphology, crystal structure, and elemental states of the modified electrode. The Ce and Nd co-doped PbO 2 electrode had smaller crystal particles, more compact structure, and higher activity of electrocatalysis compared with the single-doped and undoped PbO 2 electrodes. Linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) were also utilized to study the electrochemical response of the modified electrodes. The results show that the prepared Ce-Nd-PbO 2 electrode has the highest O 2 evolution potential (OEP) and lowest charge transfer resistance, suggesting that it has the lower energy consumption than the other three kinds of electrodes. Electrochemical oxidation methyl orange (MO) as a model dye wastewater was studied to evaluate the potential applications of this modified electrode in environmental science. It was found that the Ce-Nd-PbO 2 electrode exhibited higher MO and chemical oxygen demand (COD) removal efficiency than single-doped and pure PbO 2 electrodes.
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