MOLECULAR THERAPY
·
2026
·
IF 11.4
Peng Gu,Yunfei Zhao,Jiajia Hu,Yuguang Tian,Lingyan Zhao,Jingjing Ren,Qin Yang,Jiaxin Li,Wenhao He,Jiayin Guo,Peng Chen
Hereditary tyrosinemia type 1 (HT1) is a life-threatening metabolic disorder caused by the toxic accumulation of tyrosine and its metabolites. While treatment with nitisinone (NTBC) combined with a strict dietary regimen has improved outcomes, it imposes a significant lifelong burden and is associated with debilitating side effects and incomplete protection. Here, we developed an engineered probiotic with an optimized design, e-EcN-HT, and demonstrated its comprehensive efficacy and safety in HT1 across multiple animal models, including FAH -/- mice, FAH -/- rabbits, and Bama minipigs. Our findings indicate that e-EcN-HT not only mitigates multifaceted acute manifestations of FAH -/- mice, including neonatal death, acute liver injury, but also improves chronic liver lesions when combined with NTBC. The therapeutic effect translated successfully to the FAH -/- rabbit model. Moreover, e-EcN-HT administration led to the rapid metabolism of orally administered 13 C-tyrosine, confirming robust and active tyrosine consumption in pigs. Comprehensive safety assessments across murine and porcine models showed that e-EcN-HT was well-tolerated, with no significant adverse effects, systemic dissemination, or detrimental disruption to the resident gut microbiota. Collectively, our multi-species preclinical data underscore the potential of engineered bacteria as a viable therapeutic strategy for HT1 and possibly other metabolic disorders.
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高影响因子
WATER RESEARCH
·
2026
·
IF 12.8
Xuan Qu,Qian Xu,Zhiqiang Chen,Ang Li
Reactive oxygen species (ROS) generated on or near the oil droplet surface have remarkable potential in altering the structure of 1-adamantanecaboxylic acid (ACA) at the oil-water interface, facilitating separation of oil and water. To stimulate confined multi-phase ROS, a thermodynamic control strategy for ozone catalysis by Fe clusters anchored on nanoscale supports was developed. This strategy explored interfacial pollutant redistribution within film of microdroplets (10-20 μm in size) prepared with a high-speed shear emulsifier. Experimental results indicated that 1 O 2 selectively attacked carboxylic groups confined at the oil-water interface and two-electron migration pathway from ACA to adsorbed O 3 at interfacial oxygen vacancies (O v ) led to spatial redistribution with hydrophilic dihydroxylated intermediates via synchronous electrophilic substitution. ACA attached to interface of microdroplet is fueled by •OH and 1 O 2 , achieving degradation at 89.84% within 30 min, and pseudo-first-order kinetics rate constants ( k obs ) of 0.0793 min –1 . Thermodynamic analysis revealed that 1 O 2 reduced the Gibbs free energy (Δ G ) by 23.98 kJ mol −1 relative to that of •OH. This phenomenon is attributed to the narrow site distribution of Fe clusters, which enhances the overall electron utilization efficiency. These findings demonstrate the feasibility of manipulating ROS to regulate the distribution of interfacial species, representing a promising strategy for promoting the coalescence of oil microdroplets.
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高影响因子
POLYMER
·
2026
·
IF 5.1
Chang Liu,Yuming Cui,Yunbo Mo,Qian Xu,Kai Wang,Jianwu Lan,Shaojian Lin
Commercial poly(butylene adipate-co-terephthalate) (PBAT), a biodegradable aromatic–aliphatic copolyester, is considered a promising alternative to conventional plastics in various fields. However, its relatively low mechanical strength and low glass transition temperature (T g ) often restricts broader applications. In this study, bio-based 1,3-propanediol (PDO) was employed to completely replace 1,4-butanediol in PBAT, and a series of poly(trimethylene terephthalate- co -trimethylene adipate) (PPAT) copolyesters were synthesized via melt polycondensation. Structural characterization confirmed the targeted copolyester compositions and random chain segments (R ≈ 1), with intrinsic viscosities of 0.89-1.01 dL/g. Crystallographic analysis revealed that the crystallinity of PPATs varied with the adipic acid/dimethyl terephthalate molar ratio while preserving a monoclinic crystal structure. Notably, PPAT55 exhibited a significantly higher T g (−7.8 °C) than PBAT (−26.1 °C), and all copolyesters showed good thermal stability for melt processing. Importantly, PPAT55 fibers achieved a tensile strength of 0.71 cN/dtex after threefold drawing, representing a 51.06% enhancement over PBAT fibers. Furthermore, the increased aliphatic segment content promoted by hydrolytic susceptibility, as demonstrated by enzymatic degradation tests, with PPAT55 showing a 9.93% mass loss after 42 days, demonstrating satisfactory biodegradability comparable to PBAT. The degradation mechanism was further elucidated at the molecular level through theoretical calculations. This work provides a feasible strategy for developing high-performance sustainable polyester materials with good spinnability.
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ACS Food Science & Technology
·
2026
·Vol. 6
·期 8
·2570-2583
·
IF 3
He Wanjun,Lv Danyu,Wu Qingxiao,Xu Huanyu,Guan Yongguang,Zeng Xin-An
Litchi
( L. chinensis Sonn.) is a
perishable fruit that is vulnerable to oxidative browning and fungal
infection. Herein, ferulic acid and natamycin were chosen as the antioxidant
and antimycotic agents, respectively. To improve their water solubility,
we used CD-MOF-1 to encapsulate ferulic acid. Meanwhile, natamycin
was encapsulated with gum arabic and pullulan. The most homogeneous
and stable natamycin nanocapsules were fabricated through stirring
0.5 mg/mL natamycin using 2.5% (w/v) gum arabic-pullulan (1:1, w/w,
1.25% each) in water. The main encapsulation mechanisms of ferulic
acid and natamycin were electrostatic interactions between the carboxyl
groups of ferulic acid and K + ions from CD-MOF-1 and hydrophobic
interactions between the macrocyclic lactone ring of natamycin and
the protein part of gum arabic, respectively. In addition, pullulan
was indispensable for stabilizing natamycin. The antioxidant and antifungal
composite sol presented 99.85% oxidation inhibition and 79.06% mycelial
growth inhibition and effectively delayed the oxidative browning and
fungal decay on litchi pericarps.
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Journal of Inorganic and Organometallic Polymers and Materials
·
2026
·
IF 6
Li Qiaomei,Hong Wei,Wei Tianyu,Fan Yupeng,Luo Yuhui,Li Jinlou,Qiao Zhihua,Jia Xuemeng,Zhang Dong-En
Metal-organic frameworks (MOFs) have shown great promise for water treatment owing to their tunable porous structures and abundant active sites. However, the practical application of conventional MOFs, such as HKUST-1, is still limited by insufficient structural stability in aqueous environments and poor recoverability in powder form. Herein, a coordination-induced in situ self-assembly strategy was developed to fabricate structurally stable carboxymethyl chitosan (CMCS)@HKUST-1/polyvinylidene fluoride (PVDF) electrospun fibers. In detail, Cu 2+ was first incorporated into PVDF matrix by electrospinning to obtain Cu 2+ /PVDF fibers, which were subsequently immersed in a mixed ligand solution containing 1,3,5-benzenetricarboxylic acid (H 3 BTC) and CMCS, enabling the in situ growth of CMCS-modified HKUST-1 on the fiber matrix. CMCS participated in the coordination-induced assembly process mainly through -COOH/-COO⁻, thereby strengthening the Cu-O coordination environment and improving the resistance of HKUST-1 to water-induced structural degradation. Meanwhile, the flexible CMCS chains and their hydrophilic functional groups enhanced the interfacial interaction, wettability, and accessibility of adsorption sites within the composite fibrous network. The practical potential of CMCS@HKUST-1/PVDF electrospun fibers was evaluated through the treatment of simulated dyeing wastewater, in which it exhibited a high Congo red adsorption capacity of 173.08 mg/g and maintained robust structural stability under elevated temperature treatment at 70 ℃, as well as in aqueous environments over a pH range of 6–10. This work provides a facile and effective strategy for constructing recoverable, structurally stable, and high-performance MOF-based fibrous adsorbents for water treatment applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Chengxun Li,Fei Chen,Jianing Wu,Jun Ren,Yingzhu Li,Shengjie Li,Zuguang Xia
Antibody drug conjugates (ADCs) offer targeted cancer therapy but face limitations in drug loading, stability, and immune modulation. Here, antibody DNA nanostructure conjugates (ADNCs) is reported as a next-generation platform that overcomes these challenges. By leveraging the programmable architecture of DNA nanostructures, ADNCs achieve high, tunable drug payloads without inducing antibody aggregation—a major drawback of conventional ADCs. In CT26 cells, gemcitabine-loaded ADNCs trigger dual cell death pathways—caspase-3-mediated apoptosis and RIP3-mediated necroptosis—enhancing tumor cell killing. In the CT26 syngeneic model, ADNCs suppress tumor growth with no observable toxicity and, notably, activate anti-tumor immunity without upregulating PD-1, suggesting avoidance of T-cell exhaustion. Critically, ADNCs maintain efficacy in patient-derived xenograft (PDX) models despite tumor heterogeneity. The study presents a novel, stable, and scalable antibody-drug conjugate (ADC)-inspired platform that integrates high payload delivery capacity with enhanced and adjustable drug-loading capabilities—characteristics that are seldom achieved in existing ADC technologies—thereby demonstrating translational potential for applications in precision oncology.
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高影响因子
Foods
·
2025
·Vol. 14
·期 20
·3474
·
IF 5.1
Shaodan Peng,Lian Yang,Wei Zhou,Yuan Yuan,Liangkun Liao,Xiaobing Huang,Chenghui Zhang,Xiao Gong,Jihua Li
Lactic acid bacteria (LAB) are widely utilized in the production of various fermented foods.Lacticaseibacillus casei(Ls. casei) fermentation has been demonstrated to enhance antioxidant activity, flavor, and nutritional value. This study aimed to evaluate the physicochemical properties, proximate chemical compositions, antioxidant activities, and volatile profiles of pineapple juice fermented byLs. caseiLK-1. Strain growth, physicochemical parameters, phenolics and flavonoids, carbohydrates, organic acids (by HPLC), free amino acids (FAAs), antioxidant activities (DPPH and FRAP methods), and volatile profiles (by GC-MS) of fermented pineapple juice were characterized. After 30 h of fermentation, the cell count reached 9.07 log CFU/mL. The fermentation process led to a significant decrease in pH, total soluble solids (TSS), and thea* value (p< 0.05), with an increase in theL* andb* values (p< 0.05). Lactic acid and total umami amino acids were elevated, whereas sucrose and total sweet amino acid levels remained unchanged. The fermented juice exhibited enhanced DPPH scavenging activity and FRAP values. In terms of volatile profiles, esters and terpenes were the dominant volatiles in the fermented pineapple juice. The fermentation resulted in the production of 2-heptanone, 2-undecanone, and a significant reduction in 2-furaldehyde (p< 0.05). These findings demonstrate the feasibility of usingLs. caseiLK-1 for fermentation to develop novel fermented pineapple juice with improved antioxidant properties and a modified volatile profile.
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Journal of Extracellular Vesicles
·
2025
·Vol. 14
·期 6
·e70105
·
IF 14.5
Wenxin Zhang,Weichen Dong,Chen Cheng,Hongting Zhao,Yutong Liu,Kristina Zaydel,Liora Frech,Zhiyao Xing,Wusheng Zhu,Bing Zhou,Yi Li,Esther G Meyron‐Holtz,Kuanyu Li
The incidence of inflammatory bowel disease (IBD) is on the rise, yet current clinical treatments are limited. Previous studies have identified impairments in both systemic and local iron metabolism in IBD patients. However, the impact of iron dyshomeostasis on the development and pathogenesis of IBD remains elusive. In this study, we confirmed iron deposition in inflamed intestinal lesions of IBD patients and mice with DSS‐induced colitis, accompanied by distinct distribution patterns of the iron storage protein ferritin. To reveal the role of ferritin in the involvement of pathology of IBD, we constructed intestinal epithelial cell‐ or myeloid‐specific ferritin H ( FtH ) knockout mice and demonstrated that intestinal epithelial cells (IECs) release extracellular vesicles (EVs) that contain iron‐loaded ferritin. These EVs are internalized by macrophages via the macrophage scavenger receptor 1 (Msr1), leading to the activation of inflammatory responses and oxidative stress, thereby exacerbating colitis severity. Genetic deletion of FtH in IECs or blockage of macrophage ferritin uptake, either through Msr1 inhibitor fucoidan or through Msr1 knockdown (KD), suppressed inflammatory symptoms. Thus, EVs containing iron‐loaded ferritin released from IECs activate macrophages and contribute to IBD development, supporting that IBD patients with iron deficiency anaemia are often prescribed iron supplementation in a remission phase, other than in an active phase of the disease. Pharmacological inhibition of this ferritin secretion and engulfing process provides a therapeutic target for the disease. This study explores the interaction between intestinal epithelial cells (IECs) and macrophages through extracellular vesicles (EVs) in the context of inflammatory bowel disease (IBD). IECs release iron‐loaded ferritin via EVs as the donor, while macrophage scavenger receptor 1 on the surface of macrophages serves as the receptor for the internalization of the iron‐loaded ferritin‐containing EVs. This process activates macrophages and contributes to the development of IBD. These findings highlight the pathophysiological significance of iron metabolism disorders and suggest potential targets for future clinical applications in patients with IBD.
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Cell Death & Disease
·
2025
·Vol. 16
·期 1
·1-16
·
IF 9.6
Jing Yukai,Zhang Yunfei,Wang Xiaocui,Wang Yufen,Hu Ying,Wen Bin,Dai Xin,Duan Xuemei,Li Haonan,Dong Shumin,Yan Ze,Fan Yufeng,Wang Cong-yi,Liu Xiansheng,Zhang Ruiping
Systemic lupus erythematosus (SLE) is a typical autoimmune disease characterized by the overproduction of autoantibodies and type I interferon, which damages its own tissues, causing multiple organ damage. B cells are thought to play a major role in the pathogenesis of SLE. As a DNA methylation reader, Methyl-CpG-binding domain protein 2 (MBD2) has been extensively studied in the contexts of innate immunity, adaptive immunity, and autoimmune diseases. However, its specific role in B cells and SLE remains unexamined. Herein, we found that MBD2 was highly expressed in B cells of SLE patients and positively correlated with disease activity. Knockout of MBD2 in B cells disturbed B-cell differentiation, dampened B-cell activation, B-cell receptor (BCR) signaling, and T-cell-dependent humoral immune responses in mice. What’s more, MBD2 deficiency effectively attenuated lupus-like symptoms, reduced the germinal center responses, and decreased anti-dsDNA antibodies in lupus model mice. Mechanistically, MBD2 selectively bound to the methylated CpG of Lef-1 induced by IFN-α, inhibiting the transcription and expression of Lef-1 , which repressed Pten transcription and expression, thereby promoting PI3K-Akt-mTOR signaling. This study first demonstrated the role of MBD2 in the pathogenesis of SLE and provided a new target for SLE.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2026
·
IF 5.2
Jianan Zhang,Yangli He,Yundi Wu,Tian Xie,Wanmeng Fu,Xilong Wu,Lei Zhou,Min Zeng
Effective management of myocardial ischemia/reperfusion (I/R) injury remains a clinical challenge. Epigallocatechin gallate (EGCG), a natural antioxidant, exhibits therapeutic potential in I/R models; however, it is limited by poor bioavailability and inadequate targeted delivery. Therefore, using a synergistic therapeutic strategy, we designed a nanoplatform (PB@PEI@FPBA-EGCG NPs, referred to as PPFE NPs) to address these limitations. PPFE NPs integrate Prussian blue nanoparticles (PB NPs) with EGCG, harnessing the multi-enzyme–mimicking activities of PB NPs (SOD/CAT mimetic, •OH scavenging) together with the pharmacological properties of EGCG. Notably, in this nanoplatform, PB NPs and EGCG demonstrate excellent synergistic therapeutic effects, characterized by enhanced ROS-responsive drug release and injured cardiac tissue-selected delivery. In vitro and in vivo studies confirmed the efficacy of PPFE NPs in neutralizing ROS, restoring mitochondrial homeostasis, and inhibiting apoptosis following I/R injury. Mechanistically, cardioprotection was mediated through the regulation of DJ-1 (PARK7) and its downstream pathways—Nrf2/ARE antioxidant signaling and ASK1/p38/JNK apoptotic regulation. Importantly, this study is the first to utilize native PB NPs as an EGCG carrier, exploiting their ROS scavenging ability and boronate ester bonds to improve EGCG loading efficiency and targeted delivery. Furthermore, we innovatively found that the combination of EGCG and DJ-1 may represent a key mechanism underlying the protective effects of EGCG against myocardial ischemia/reperfusion injury (MIRI). This work pioneers a ROS-responsive Prussian blue (PB) nanoplatform with superior synergistic efficacy. The study establishes PPFE NPs as a biocompatible nano-platform for myocardial I/R injury and provides a translatable strategy to optimize the clinical application of EGCG.
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DESIGNED MONOMERS AND POLYMERS
·
2014
·
IF 2.21
Bo Gao,Ping Guan,Xiaoling Hu,Liwei Qian,Qiaoli Wang,Longfei Yang,Dan Wang
L-Phenylalanine (L-Phe) imprinted polymers (L-MIPs) were prepared via precipitation polymerization using 1-vinyl-3-carboxymethylimidazolium chloride ([VCIM]Cl) as functional monomer, which could interact with L-Phe through electrostatic interactions, hydrogen-bonding, and π–π interactions. The morphologies of obtained polymers were observed by a scanning electron microscope, and their structures were analyzed by Fourier transform infrared spectroscopy. The effects of monomer species and molar ratio of template to functional monomer or crosslinker on adsorption capacity and imprinting factors (IFs) of polymers were investigated. The result indicated that [VCIM]Cl was preferable monomer and the optimum molar ratio of template to functional monomer and crosslinker was 1:4:16. The maximum IF of the obtained L-MIPs could reach 3.86 in 0.4 mg mL−1 L-Phe solution at 25 °C, after 8 h of adsorption and the maximum adsorption capacity of L-MIPs for L-Phe was 33.08 mg g−1. The adsorption behavior of L-Phe on the L-MIPs was construed seriously within the experimental concentration by Freundlich model and Langmuir model. In terms of the higher correlation coefficient, it was considered that the adsorption behavior of L-Phe fit Freundlich model better than Langmuir model, which means that the distribution of active sites was heterogeneous in L-MIPs. The selectivity adsorption showed L-MIPs have better specificity and selectivity for L-Phe than that of L-His and L-Trp. Finally, L-MIPs were applied to the CD and Automatic Polarimeter experiments, showing that L-MIPs had the capability of specific chiral recognition for L-Phe.
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ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE
·
2017
·Vol. 643
·期 7
·471-476
·
IF 1.144
Lingyun Dong,Jiao Yang,Shigang Shen,Zhenzhen Liu,Xiaojing Chen,Sufang Sun
Through extensive research on the PbO / PbBr 2 / B 2 O 3 system, a new single crystal of yellow lead-containing oxyborate bromine, [O 2 Pb 3 ] 2 (BO 3 )Br, was grown from the melt. It crystallizes in the centrosymmetric space group Cmcm (no. 63) of the orthorhombic system with the following unit cell dimensions: a = 9.5748(8) Å, b = 20.841(2) Å, c = 5.7696(5) Å, and Z = 4. The whole structure is characterized by an infinite one-dimensional (1D) ∞ 1 [O 2 Pb 3 ] double chain, which is based on the OPb 4 oxocentered tetrahedra and considered as the derivative of the continuous sheet of OPb 4 tetrahedra from the tetragonal modification of α-PbO. The 1D ∞ 1 [O 2 Pb 3 ] double chains are further bridged by the BO 3 units through common oxygen atoms to form two-dimensional (2D) ∞ 1 [[(O 2 Pb 3 )(BO 3 )] layers, with Br atoms situated between the layers. IR spectroscopy, UV/Vis/NIR diffuse reflectance spectroscopy, and thermal analysis were also performed on the reported material.
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危险品化学品经营许可证（带存储）