ANALYTICAL LETTERS
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2011
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IF 0.92
Chuanping Wei,Huiqing Zhou,Changbao Chen,Zongchen Li,Jie Zhou
A surface plasmon resonance (SPR)-based detection methodology, which allows the identification and quantification of the plant hormone, 1H-indole-3-acetic acid (IAA) in Peach, Rosa flower, and Crape myrtle bud is described. To achieve high selectivity, a molecularly imprinted monolayer (MIM) was prepared from alkanethiols self-assembled on an SPR sensor chip with preadsorbed template (IAA). The association constant of the MIM to IAA was calculated to be 2.8 × 1011 M−1 by analysis of the SPR wavenumber changes induced by different concentrations of IAA. A good calibration curve was obtained in a low concentration range of IAA. Moreover, the MIM revealed impressive selectivity with the selectivity factors of 1.0, 0.12, and 0.20 for IAA and other related analogues. Compared to the MIM sensor chip, the affinity of the self-assembled monolayer (SAM) control sensor chip for IAA was significantly low. The single SPR sensor decorated with the MIM was used to determine IAA concentrations in peach, Rosa flower, and Crape myrtle bud, and satisfactory results were obtained. Based on a signal to noise ratio of 3, the detection limits were 0.23 pM for Crape myrtle, 0.20 pM for Peach, and 0.32 pM for Rosa. The method showed good recoveries and precision, 95.7% (RSD 0.54%) for Peach, 98.1% (RSD 3.4%) for Rosa, and 98.1% (RSD 2.5%) for Crape myrtle, spiked with 0.4171 µg, respectively. These results indicate that a combination of SPR sensing and the MIM provides a simple, promising alternative method for determining endogenous IAA in plant tissue in a real-time manner.
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高影响因子
JOURNAL OF POWER SOURCES
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2024
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IF 8.1
Lu Sai,Zhongsheng Dai,Zhenbo Wang,Huiling Zhao,Ying Bai
Li-rich layered oxides with high energy densities have become one kind of promising cathode for advanced lithium-ion batteries, however some issues are severely hindering their practical applications. To address these challenges, fast ion conductive LiTi 2 (PO 4 ) 3 (LTPO) is employed to modify the typical Li-rich cathode Li 1 · 2 Mn 0 · 56 Ni 0 · 17 Co 0 · 07 O 2 (LMNCO) via a sol-gel process combined with a two-step annealing treatment in this work. Serving as a physical separator, LTPO coating effectively depresses the side reactions at cathode-electrolyte interface and the transition-metal dissolution from LMNCO active material under harsh electrochemical environment. Meanwhile, LTPO layer with high ion conductivity could facilitate the interfacial Li + diffusion during charge/discharge process. In addition, partial Ti 4+ ions doped into LMNCO effectively elevate the oxygen electronegativity and restrain the excessive oxidization of lattice oxygen, thus stabilizing the crystal structure and alleviating the stress-strain propagation upon cycling. Electrochemical characterization results demonstrate that the LTPO-modified LMNCO cathode exhibits a superior capacity retention of 86.5 % after 400 cycles at room temperature and that of 73.7 % even under an elevated temperature of 45 °C after 250 cycles under 1C. This study provides a facile strategy for the surface modification of Li-rich layered-structure oxides, which also sheds light in enhancing electrochemical performances for various cathode materials .
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ARCHIVES OF TOXICOLOGY
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2024
·
IF 4.8
Yang Liu,Li Qiao,Wang Sa,Ji Yi,Ma Xinbo,Qin Ming,Gao Yanhui,Yang Yanmei
Skeletal fluorosis is a chronic metabolic bone disease caused by long-term excessive fluoride intake. Abnormal differentiation of osteoblasts plays an important role in disease progression. Research on the mechanism of fluoride-mediated bone differentiation is necessary for the prevention and treatment of skeletal fluorosis. In the present study, a rat model of fluorosis was established by exposing it to drinking water containing 50 mg/L F − . We found that fluoride promoted Runt-related transcription factor 2 (RUNX2) as well as superoxide dismutase 2 (SOD2) and sirtuin 3 (SIRT3) expression in osteoblasts of rat bone tissue. In vitro, we also found that 4 mg/L sodium fluoride promoted osteogenesis-related indicators as well as SOD2 and SIRT3 expression in MG-63 and Saos-2 cells. In addition, we unexpectedly discovered that fluoride suppressed the levels of reactive oxygen species (ROS) and mitochondrial reactive oxygen species (mtROS) in osteoblasts. When SOD2 or SIRT3 was inhibited in MG-63 cells, fluoride-decreased ROS and mtROS were alleviated, which in turn inhibited fluoride-promoted osteogenic differentiation. In conclusion, our results suggest that SIRT3/SOD2 mediates fluoride-promoted osteoblastic differentiation by down-regulating reactive oxygen species.
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APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Tao Huanga,Lifeng Guan,Shuai Jin,Dehui Zhang,Wangwen Liao,Xiangyang Zhou,Xingxing Liu,Chen Wu,Wei Zeng
The low electrical conductivity of Li 3 V 2 (PO 4 ) 3 (LVP) impedes its practical application. To address this issue, this study enhances its conductivity by introducing nitrogen-doped carbon nanoframes into LVP. Using phytic acid as a carbon and phosphorus source, and polyvinylpyrrolidone (PVP) as a nitrogen source, nitrogen-doped nanoframe-modified LVP material (LVP@NC) was successfully synthesized. Importantly, the carbon nanoframes of LVP@NC are entirely derived from the precursor’s thermal decomposition, simplifying the synthesis process and reducing production costs. By optimizing the process parameters, significant improvements were achieved in key performance indicators, including the initial discharge capacity at 1C (132.85 mAh·g −1 ), long cycling life at 10C (98.98 % capacity retention after 1000 cycles), and rate capability at 50C (99.65 mAh·g −1 ). These improvements are attributed to the introduction of nitrogen-doped nanoframes, where the high-temperature decomposition of PVP leads to the formation of porous honeycomb-like structures, and nitrogen doping in the nanocarbon framework introduces defects and active sites, thereby promoting lithium-ion migration and facilitating electrolyte penetration. This simple, novel, and energy-efficient method demonstrates significant potential for the large-scale preparation of high-performance cathode materials.
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ACS Sustainable Chemistry & Engineering
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2024
·
IF 7.1
Bo Yang,Wentao Sun,Xinhe Liu,Ming-zhu Ding,Xudong Feng,Chun Li
Glycyrrhetinic acid 3-O-monoglucuronide (GAMG) and glycyrrhizin (GL) are typical pentacyclic triterpenoid saponins present in licorice roots that exhibit important pharmacological properties. GAMG and GL are sourced primarily from licorice, namely, “digging roots and extracting acid”, which results in ecological imbalance and environmental pollution. To overcome these issues, it is an eco-friendly and sustainable alternative to de novo synthesize GAMG and GL in Saccharomyces cerevisiae by coupling UDP-glucuronic acid and glycyrrhetinic acid (GA) under the catalysis of glycosyltransferases. However, the reported productivity is too low for industrial production. The primary limiting factors include insufficient supply of precursors, poor activity, and low specificity of the glycosyltransferases. In this study, we reconstructed the GA-producing platform strain in which GA was mainly accumulated intracellularly, making it suitable for subsequent glycosylation modifications. Different glycosyltransferases were tested and selected in the platform strain for achieving the efficient and specific synthesis of GAMG and GL. Protein engineering of rate-limiting enzyme cUGT73P12 was performed to further facilitate the conversion from GAMG to GL. The glycosylation capacity of this system was assessed in vivo, uncovering that the insufficient supply of precursor GA limited glycosylation modifications. Correspondingly, multiple metabolic engineering strategies were applied to optimize the carbon flux distribution, increasing the titers of GAMG and GL by 115.6 and 50.2%, respectively. Finally, 552.9 mg/L GAMG and 476.6 mg/L GL were produced in the 5 L fed-batch fermentation, which were 238- and 79-fold higher than previously reported values, respectively. The strategy described herein can serve as a reference for the glycosylation of triterpenoids in a cost-effective manner.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
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IF 3.8
Zhang Shirong,Tan Yuxin,Liu Yilei,Li Yafei,Li Jishun,Zhang Shenglan,Pan Hongcheng
Quantum dots (QDs) have emerged as highly sensitive fluorescent markers for lateral flow immunoassays (LFIAs) due to their excellent fluorescence brightness and photostability. The incorporation of multiple QDs onto a carrier can further enhance detection sensitivity. However, traditional carriers such as polystyrene, while commercially successful, involve complex preparation processes and rely on organic solvents, which can lead to fluorescence quenching and adversely affect detection performance. In this study, we selected isoreticular metal–organic framework-3 (IRMOF-3), characterized by its porous structure, high surface area, and abundant amine functional groups, as the carrier. We synthesized IRMOF-3@CdSe/ZnS composite nanoparticles with an average diameter of 378 ± 46 nm by coupling carboxyl-modified CdSe/ZnS QDs to the IRMOF-3 surface through an aqueous synthesis method. These nanocomposites efficiently loaded a high density of QDs without aggregation, thereby maintaining strong fluorescence intensity. Moreover, the abundant amino and carboxyl groups on their surfaces facilitate efficient antibody conjugation, and the nanoparticles retain excellent water dispersibility after labeling. Utilizing IRMOF-3@CdSe/ZnS composite nanoparticles as immunomarkers, we developed a novel fluorescent LFIA for the detection of interleukin-6 (IL-6), a biomarker associated with various inflammatory and autoimmune diseases. The method achieved a limit of detection for IL-6 as low as 8.6 pg/mL, with relative standard deviations ranging from 2.73% to 6.14% and spiking recoveries between 96.4% and 110.8%. Passing-Bablok regression analysis demonstrated good consistency between our method and commercially available immunochromatography assays. The IRMOF-3@CdSe/ZnS composite nanoparticles, as a novel fluorescent label, exhibit considerable potential for applications in biomedical detection. Graphical abstract
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SENSORS AND ACTUATORS B-CHEMICAL
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2025
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IF 7.7
Xingxing Yu,Faxun Wang,Huakang Zong,Yufei Gan,Guotao Duan,Yuanyuan Luo
The modification of ionic liquid electrolytes is of particular importance for the development of high-performance electrochemical oxygen sensors with zero power consumption but still quite challenging. Herein, we designed and synthesized an electrochemical oxygen sensor based on the modified Pt/CMK-3-COOH-[EMIM][OAc] ionic liquid as composite electrolyte, where the -COOH functional group was introduced by acidifying CMK to provide hydrogen protons, and then Pt nanoparticles with different contents were loaded to improve the activity of the sensor towards O 2 gas as well as the conductivity of ionic liquid. Such composite ionic liquid sensor exhibits excellent O 2 detection ability with high sensitivity (0.58 μA/[%O 2 ]), high stability, wide detection range (0.4-10%), and zero-power consumption, along with the detection limit as low as 0.4%. Furthermore, it has humidity-independent gas-sensing characteristic. Moreover, density functional theory (DFT) calculations combined with experimental results were applied to deeply unveil the improved O 2 sensing mechanism. Compared with CMK, the introduction of Pt/CMK-3-COOH material increases the conductivity of the ionic liquid, decreases the adsorption energy of the sensor for O 2 and hydrogen protons, promotes the oxygen reduction reaction, and simulates the transfer of effective charge, thereby significantly improving O 2 sensing. This work provides a high-performance ionic liquid oxygen sensor, showing potential application in zero-power consumption and low concentration O 2 detection.
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Nano Energy
·
2021
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IF 17.881
Guijia Cui,Qingyu Dong,Zhuangzhou Wang,Xiao-Zhen Liao,Siqi Yuan,Meidan Jiang,Yanbin Shen,Hong Wang,Haiying Che,Yu-Shi He,Zi-Feng Ma
NASICON-type (Na super ionic conductor) Na 4 VMn(PO 4 ) 3 (NVMP) cathode material has attracted increasing attention due to its higher sodium de-/intercalation voltage, lower cost and greener resources compared with Na 3 V 2 (PO 4 ) 3 . However, the poor electronic conductivity limits the exploitation of its electrochemical performance. In this work, we achieved an ultrahigh rate capable and long cycle life NVMP/C-rGO composite using the simple and scalable spray-drying technique, in which low-fraction rGO nanosheets (2.14 wt%) highly dispersed and bridged the carbon shelled NVMP nanoparticles to form an effective electron conductive network. Benefiting from this favorable nano-architecture, the elaborately designed NVMP/C-rGO composite exhibited excellent performance as cathode in sodium ion batteries, delivering reversible capacities of 105.5 mA h g −1 at 1 C (1 C = 110 mA h g −1 ) and 102.6 mA h g −1 at 50 C with capacity retention of 90.6% (1 C) and 80.4% (50 C) over 500 cycles, respectively. This is the highest rate performance for Na 4 VMn(PO 4 ) 3 materials reported so far. This work provides a practicable tactic for fabricating advanced polyanion-type cathode materials which can be commercially scale up for sodium energy storage.
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CRYSTENGCOMM
·
2024
·Vol. 26
·期 9
·1303-1311
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IF 2.6
Shuyue Miao,Fangbao Jiao,Ying Wang,Qi Zhang,Suming Jing,Hongzhen Li,Xin Zhou
Compared with jagged and rod-like raw products, the spherulitic 3-nitro-1,2,4-triazol-5-one (NTO) crystalline powder is generally superior in the formulation. However, the growth mechanism and corresponding influential factors for NTO spherulites remain to be deeply explored. Herein, the morphological evolutions of NTO crystals in alcohol solvents under a range of maximum supersaturation (Smax) are systematically investigated. It was found that differences in solvent-crystal interface interactions determine the primary morphology of the growing crystals while the magnitude of Smax determines the subsequent polycrystalline evolution. The cooling rate, initial concentration and stirring rate all could affect the crystal morphology by changing the Smax. It is demonstrated that there exists a critical Smax range (1.75–1.88) above which the formation of spherulites is kinetically favorable. Face indexing tests suggest that the (0 0 1) plane of NTO is mostly prone to twinning at low supersaturation, which is because that the molecular layers on the (0 0 1) surface are prone to twisting and the energy difference before and after is only 8.77 kJ mol−1. Then, the interfacial growth theory is employed to explain how a growing crystal plane develops from smooth to rough with non-crystallographic sub-individuals as the crystallization driving force increases. The change of morphology has a positive consequence in improving the safety performance of NTO crystalline powder under drop-weight impact. These findings would forward the understanding of the spherulitic crystallization process for energetic crystals.
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JOURNAL OF POLYMER ENGINEERING
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2020
·Vol. 40
·期 5
·409-414
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IF 1.126
Tian-Xiang Jin,Miao-miao Wu,Jin-bo Ou-yang,Qin Zhang
Poly (butylene succinate-co-butylene-3-hydroxyphenylphosphinyl-propionate) (PBSH) was synthesized through polycondensation. Then, 4,4′-methylene diphenyl diisocyanate (MDI) was introduced into PBSH matrix by reactive blending. The chemical structure and properties of the blending products were investigated using Fourier Transform Infrared Spectroscopy (FT-IR), differential scanning calorimetry (DSC), limiting oxygen index (LOI) tests, thermogravimetric analysis (TGA), and vertical burning tests. The results proved that MDI can improve the mechanical properties and flame retardancy of PBSH. In addition, it was found that the crosslinking structure can reduce the hydrolysis rate of PBSH and effectively eliminate the melt-dripping of PBSH during combustion.
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高影响因子
Journal of Cleaner Production
·
2019
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IF 6.395
Hossain M. Zabed,Yufei Zhang,Qi Guo,Junhua Yun,Miaomiao Yang,Guoyan Zhang,Xianghui Qi
The probiotic bacterium, Lactobacillus reuteri, possesses a unique pathway for metabolizing glycerol into 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PD), which are two important platform chemicals. In this work, a potent L. reuteri strain, designated as FXZ014, was isolated and identified from healthy human feces for co-biosynthesis of 3-HP and 1,3-PD during whole cell biotransformation of glycerol. Initially, biotransformation conditions were optimized for this new strain using single factor optimization and response surface methodology (RSM). Under the optimum batch conditions, FXZ014 produced 8.74 ± 0.24 g/L of 3-HP and 9.94 ± 0.36 g/L of 1,3-PD with a corresponding net yield of 0.67 mol/mol. Finally, efficiency of the isolated strain in the co-production of these two metabolites was compared with the same of a reference strain, namely L. reuteri CICC 6118. Compared to the reference strain, the amounts of 3-HP and 1,3-PD produced by the isolated strain were significantly higher that corresponded to 38.4% and 21.14%, respectively. Therefore, FXZ014 could be a promising natural strain for co-biosynthesis of 3-HP and 1,3-PD from glycerol.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2017
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IF 2.603
Yang Xu,Dongwen Hu,Yuting Li,Chongde Sun,Wei Chen
Accumulating evidence indicates that consumption of berries may exert beneficial effects against oxidative stress mediated diseases. Pelargonidin -3-O- glucoside (Pg3G), a bioactive ingredient in strawberry, has been reported to possess a potent antioxidant capacity. This study was therefore designed to develop an effective method to prepare pure Pg3G from strawberry and investigate its protective effect against H 2 O 2 -induced oxidative stress. According to our results, Pg3G occupied 85.55% of total anthocyanin content in strawberry. 240 mg of Pg3G with the purity of 97.26% was finally isolated from 320 g of strawberry lyophilized powder (SLP) by combination of AB-8 macroporous resin and high-speed counter-current chromatography (HSCCC) technologies. Further study unveiled that Pg3G significantly inhibited H 2 O 2 -induced ROS generation, GSH depletion and mitochondrial dysfunction, thereby ameliorating H 2 O 2 -induced oxidative stress. Overall, this study suggests that pelargonidin -3-O- glucoside can be used as a natural bioactive agent to prevent cellular oxidative stress.
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高影响因子
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