ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2026
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IF 4.1
Hu Jiale,Li Wei,Luo Pei,Nan Yihong,Liu Yimin,Yang Yashi,Zou Jianmei,He Huijun,Zhang Yun
In this work, an innovative Tyndall-effect-based (TE-based) colorimetric strategy was developed to detect the activity of alkaline phosphatase (ALP) via enzyme-triggered degradation of MnO₂ nanosheets (MnO₂ NSs), and it was further extended to construct a TE-based colorimetric enzyme-linked aptamer assay (ELAA) for monitoring thrombin (TB) concentrations. In the presence of ALP, ascorbic acid 2-phosphate (AAP) was hydrolyzed to ascorbic acid (AA), which in turn reduced MnO₂ NSs to Mn 2+ and led to their structural decomposition. Under the irradiation of a handheld laser pointer, the TE signal of the detection solution gradually diminished with increasing ALP concentration. The quantitative measurement of ALP was further achieved using a smartphone to read out the TE signal, with a limit of detection (LOD) of 0.35 mU/mL. Furthermore, this strategy effectively functioned as an exquisite signal generation mechanism to devise a TE-based colorimetric ELAA for TB detection. The results demonstrated that the proposed method exhibited high specificity and sensitivity for TB, capable of linear detection over a concentration range of 1–25 nM with a LOD of 0.38 nM (3σ). In actual human serum samples, the recovery of TB ranged from 93.40 to 109.30%. To our knowledge, this work presents the first TE-based colorimetric ELAA for instrument-free protein analysis by leveraging enzyme-triggered MnO₂ nanosheet degradation. This approach establishes a novel signal transduction mechanism for colorimetric ELAA, thereby offering significant potential to expand the theoretical scope of this assay format and broaden its applications in various resource-limited settings. Graphical abstract
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Nature Communications
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2026
·
IF 18.1
Xiao Jin,Li Yang,Liu Yanan,Li Jing,Fang Li,Zheng Haofeng,Wang Yanlong,Liu Qi,Ma Xuyu,Pang Shuai,Hu Jing,Wang Jianbo,Hou Shaocong
Precise control of interfaces is crucial for spin generation, transport, and detection in opto-spintronics. However, the interface engineering for efficient spin injection remains a significant challenge. Here, we synthesized a helical structure of PbI 2 ( R -PbI 2 ) via an interfacial chirality-induced growth approach at the heterostructure interface. This few-nanometer-thick R -PbI 2 layer shows a lower lattice mismatch with both the adjacent R- NEAPbI 3 ( R- NEA refers to R -1-(1-naphtyl)ethylamine) and PbI 2 layers, and leads to an optimal chiral interface in the chiral heterostructure with minimized residual strain and defect density. Combined with circularly polarized pump-probe spectroscopic and spin-photovoltaic measurements, our chiral heterostructure interface contributes a spin-injection efficiency up to 68%, thus leading to a degree of polarization of 29% in photocurrent. The precise synthesis of a chiral interface offers a promising route to manipulate spin dynamics and achieve a high degree of spin polarization required for advanced opto-spintronic applications.
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Microfluidics and Nanofluidics
·
2026
·Vol. 30
·期 3
·21
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IF 2.9
Liu Zhen,Chen Rifei,Huang Kaicheng,Zhu Haiping,Liu Jiaze,Wu Chunhui,Wang Liqiu,Cheng Xing
Digital microfluidics (DMF) shows a great application prospect in droplet manipulation. However, the fouling of the hydrophobic surfaces caused by biomolecules limits its development. In this study, we report a new strategy to enhance the functionality and anti-biofouling performance of the DMF chip by using a hydrophobic liquid surface (HLS) rather than a regular hydrophobic solid surface (HSS). The DMF chip with such a configuration can efficiently drive various liquids with full-function operations. Moreover, our DMF chips can directly manipulate biomolecular droplets without restrictive conditions like adding surfactants or filling with silicon oil. The liquid-liquid contact between the droplet and the hydrophobic surface ensures that the non-specifically adsorbed biomolecules move along with the droplet. Thus, no residue is left behind to ruin the hydrophobicity of the hydrophobic surface. Meanwhile, the long-term reversibility of contact angle change and stability of droplet movement demonstrate the excellent ability against biofouling. In addition, high- and low-temperature tests also show the temperature stability of the HLS. Finally, a biochemical application, plasmid extraction of Escherichia coli ( E.coli ) cells, is successfully carried out on the DMF chip with HLS as a proof of its usability. This HLS is expected to offer versatile functionalities and anti-biofouling performance for DMF chips in handling biomolecular droplets.
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Nature Energy
·
2026
·
IF 70.1
Zhao Qiangqiang,Zhang Bingqian,Hui Wei,Gao Kun,Ji Hongpei,Sun Xiuhong,Feng Xiaopeng,Peng Cheng,Wang Kaiyu,Gao Caiyun,Zhang Chenyang,Wang Han,Zhang Qi,Su Zhenhuang,Wang Xiao,Wang Li,Pang Shuping,De Wolf Stefaan,Wang Kai
Incorporating two-dimensional (2D) perovskite phases at the interfaces of three-dimensional (3D) perovskite solar cells improves device performance. These 2D structures form in the bulk and at interfaces of the perovskite film by adding long-chain ammonium salts into the perovskite, but achieving them exclusively at the buried interface remains challenging. Here we sequentially graft thioglycolic acid and oleylamine onto SnO 2 nanoparticles. The strong chemical bond between thioglycolic acid and oleylamine ensures that cation exchange with formamidinium iodide, a perovskite precursor, occurs only during thermal annealing, creating a 2D/3D perovskite structure solely at the bottom interface. This localized 2D layer accelerates 3D phase formation, enhances perovskite crystallization and reduces defect concentration at the interface by over tenfold. The resulting solar cells achieve power conversion efficiencies of 26.19% (certified 26.04%, 0.09 cm 2 ), 23.44% (aperture area of 21.54 cm 2 , certified 22.68%) and 22.22% (aperture area of 64.80 cm 2 ), respectively.
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Cell Communication and Signaling
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2026
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IF 8.9
Qiu Yueping,Liu Yijie,Ding Haiying,Xin Wenxiu,Lu Shuanghui,Hu Yan,Chen Jing,Weng Qinjie,Wang Jiajia,Fang Luo
Osimertinib is a first-line treatment for advanced non-small cell lung cancer with epidermal growth factor receptor (EGFR)-sensitive mutations; however, the severe hepatotoxicity of osimertinib is a challenge that has attracted attention. In this study, we found that osimertinib directly caused hepatocyte damage, with cells showing morphological features of vacuolization after treatment. Using transmission electron microscopy and other analytical techniques, we found that excessive activation of the autophagy-lysosome pathway was the main cause of cell vacuolation. Mechanistically, osimertinib causes dephosphorylation of transcription factor EB (TFEB), leading to its translocation into the nucleus to exert transcriptional regulatory functions. TFEB in the nucleus induces autophagy and lysosome biogenesis, thereby causing excessive activation of the autophagy-lysosome pathway subsequently leading to vacuolation-associated cell death. Through this study, we have identified S-adenosyl-L-methionine as a clinical hepatoprotective agent that could mitigate osimertinib-induced hepatocyte damage by inhibiting TFEB activation. Collectively, our findings not only lay the groundwork for addressing the clinical challenges posed by osimertinib hepatotoxicity but also broaden our understanding of drug-induced hepatocyte injury, thereby offering a novel research pathway for the study of drug-induced liver injury.
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Nature Communications
·
2026
·
IF 18.1
Jiang Yi,Wang Banghai,An Yang,Liu Tianji,Qin Rui,Zhu Dong,Zhang Min,Chen Zipeng,Yang Zhengwei,Li Wei,Li Qiang,Chen Peng,Lu Yanqing,Zhu Jia,Zhu Bin
Various outdoor scenarios demand both temperature control and self-protection from environment, which are often contradictory from the optical perspective, thus inspiring many material designs on multispectral camouflage and radiative cooling performance. However, these methods on the basis of one-dimensional photonic crystals or meta-surfaces always rely on stringent fabrication and may result in strong angular dependence. Here, we demonstrate an aluminum-polyamide 66 metal-based polymer bilayer thin film through hierarchical design at both the molecular and microscale levels and scalable production, enabling camouflage in infrared (3-5 μm and 8-14 μm) and laser (10.6 μm) bands with efficient radiative cooling in the non-atmospheric window (5-8 μm and 14-20 μm) while possessing weak angular dependence between −60° to 60°. Furthermore, our films can be tailored with specific emissivity and color to balance camouflage and cooling across diverse environments. This work provides a scalable, low-cost radiative cooling polymer film, advancing practical solutions for multispectral camouflage.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 3
·126
·
IF 5.4
Zhou Yu,Wu Runqiang,Fan Lingling,Qui Pengtao,Deng Xiaohua,Tan Xiaohong,Dong Shuaikang,Li Canru,Chen Jiejing,Liang Jintao,Li Guiyin
Hepatocellular carcinoma (HCC) is one of the most lethal malignancies worldwide, and early diagnosis is crucial. Golgi protein 73 (GP73) has emerged as a promising serum biomarker for HCC. However, current detection methods often fail to meet routine screening requirements due to limitations in sensitivity and operational simplicity. To address these challenges, we have developed a novel fluorescent aptamer-based sensor for highly sensitive GP73 detection based on the fluorescence resonance energy transfer (FRET) mechanism between graphitic carbon nitride quantum dots (g-CNQDs) and a copper-based metal–organic framework (Cu-TCPP). g-CNQDs were covalently conjugated with a GP73-specific aptamer to serve as the fluorescent donor, while two-dimensional Cu-TCPP nanosheets acted as the efficient acceptors. Fluorescence was quenched upon donor–acceptor interaction via FRET. In the presence of GP73, aptamer–target binding disrupted FRET interaction, separating the donor from the acceptor and restoring fluorescence in a concentration-dependent manner. Under optimal conditions, the sensor exhibited excellent linearity over a concentration range 1.0–225.0 ng mL⁻¹, with a detection limit as low as 0.907 ng mL⁻¹. Recoveries for spiked human serum samples ranged from 95.96% to 103.85%, with relative standard deviations (RSDs) of 0.38%–5.48%. The developed aptamer sensor demonstrated excellent sensitivity, selectivity, and stability, providing a powerful tool for early HCC diagnosis and offering strong potential for real-time analytical applications. Graphical A novel fluorescent aptamer sensor for GP73 detection by leveraging the fluorescence resonance energy transfer (FRET) mechanism between graphite-phase carbon nitride quantum dots (g-CNQDs) and a copper-based organic framework (Cu-TCPP). This platform achieved a good linear relationship with GP73 concentration in the range of 1.0–225.0 ng mL -1 for GP73 concentration, and a detection limit of 0.907 ng mL -1 .
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JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 12.6
Li Cai,Jian Gao,Kai Zhang,Bing Xiao,SiJia Xu,Wei Zhao,Juan Li,Yanli Zhou,WenYing Zhu,ShuYuan Liu,TingTing Pei,JunHua Li,Yang Chen,ShiXian Chen,Ji Li,Juan Li
Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic synovial inflammation, cartilage destruction, and bone loss. Current therapeutic approaches are often limited by short drug half-life, insufficient local drug release, and substantial systemic side effects.In this study, we developed a composite thermosensitive hydrogel system that integrates in situ gelation, sustained drug release, and multitarget therapeutic effects for localized precision treatment of RA.The system consists of a thermosensitive hydrogel matrix composed of hydroxypropyl methylcellulose (HPMC), hyaluronic acid (HA), and glycerol, in which gelatin methacryloyl (GelMA) hydrogel microspheres are embedded. The microspheres efficiently encapsulate Drynaria rhizome-derived extracellular vesicles (DR-EVs), while sinomenine is incorporated into the thermosensitive hydrogel to enhance anti-inflammatory activity. Characterization by transmission electron microscopy (TEM), scanning electron microscopy (SEM), nanoparticle tracking analysis (NTA), rheological measurements, and Fourier-transform infrared spectroscopy (FTIR) confirmed the intact morphology of DR-EVs, the uniform porous structure of the microspheres, and the favorable thermoresponsive gelation behavior and controllable degradation properties of the composite system. Functional assays revealed that, in vitro, the system effectively suppressed TH17 cell proliferation, promoted Treg cell differentiation, and inhibited M1 macrophage polarization.Meanwhile, it upregulated osteogenesis-related genes (Runx2, BMP2) and inhibited osteoclast formation. In a collagen-induced arthritis (CIA) rat model, the system significantly alleviated joint swelling, restored cartilage and bone architecture, and suppressed the progression of synovial inflammation. In summary, this composite thermosensitive hydrogel system possesses injectability, thermoresponsive behavior, prolonged release capability, and multiple biological activities, offering a safe, efficient, and controllable novel strategy for localized precision therapy of RA.
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RESPIRATORY RESEARCH
·
2026
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IF 5
Zhang Jia,Liu Jin,Chen Yuqian,Chen Huan,Meng Yihan,Wang Yan,Qiu Yuanjie,Hu Huizhong,Shi Wenhua,Li Manxiang,Li Man-xiang
Background Galactose lectin-3 (Gal-3) has been shown to promote the progression of pulmonary arterial hypertension (PAH), yet the intricate molecular mechanisms are still unclear. Methods Primary cultured rat pulmonary arterial smooth muscle cells (PASMCs) and PAH rats were used in this study. Protein phosphorylation and expression levels were identified using Western blotting, while mRNA level was quantified through qRT-PCR. Hemodynamic measurements, hematoxylin-eosin and immunohistochemistry staining were employed to assess the PAH progression. Results Yes-associated protein 1 (YAP1) mediated Gal-3/toll-like receptor 4 (TLR4)-induced PDZ-binding kinase (PBK) upregulation. Furthermore, polo-like kinase 1 (PLK1)/cyclin-dependent kinase 1 (CDK1) mediated Gal-3/TLR4-induced PBK phosphorylation. Activated PBK further phosphorylated protein regulator of cytokinesis 1 (PRC1), which ultimately led to PASMC proliferation. In monocrotaline-induced PAH rat models, inhibition of Gal-3, TLR4, YAP1, CDK1 or silencing of PLK1, PBK, PRC1 attenuated PAH progression. Conclusion Our study presents novel evidence that Gal-3 promotes PASMC proliferation and pulmonary vascular remodeling by activating PBK/PRC1 through the TLR4/YAP1 and TLR4/PLK1/CDK1 signaling pathways, suggesting that this signaling pathway might be a promising target for the treatment of PAH.
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JOURNAL OF FLUORESCENCE
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2026
·
IF 3.1
Zhu Danhong,Wu Wanhui,Zhang Lejing,Liu Zixin,Zhao Huan,Zheng Zhongkai,Lin Mei
The development of fluorescent probes with high stability and selectivity for ferric ion (Fe³⁺) detection remains crucial for environmental monitoring. Herein, we report a one-pot hydrothermal synthesis of nitrogen and sulfur co-doped carbon dots grafted with polyvinylamine (CDs-PVAm) using citric acid and L-cysteine as sustainable precursors. The obtained CDs-PVAm exhibited strong blue fluorescence with a high quantum yield of 49% and an average fluorescence lifetime of 9.75 ns, attributed to the synergistic effect of N, S co-doping and PVAm passivation which effectively suppresses non-radiative recombination. The material demonstrates excellent environmental stability over a wide pH range (3–9), under thermal stress (25–45 °C), and prolonged irradiation. As a fluorescent probe, CDs-PVAm shows high selectivity toward Fe³⁺ among various metal ions, with a linear response range of 0–200 µM and a detection limit of 11.40 µM. Practical application in natural water samples achieved satisfactory recoveries (100.46–101.45%) with low relative standard deviations (0.71–1.43%), confirming its reliability for Fe³⁺ detection in complex aqueous matrices. This work presents a robust nanoplatform for environmental sensing with significant application potential.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 2
·124
·
IF 5.4
Li Zhe,Gao Zhaodong,Chen Zhijiao,Liu Yuanan,Wang Xiaohui
A dual-mode ratiometric fluorescent nanosensor was developed for ultrasensitive detection of glucose via simultaneous measuring pH and O 2 changes during enzymatic oxidation. The nanosensor integrates pH-sensitive fluorescent probe FITC and O 2 -sensitive probe RuDP into hybrid nanoparticle core, with glucose oxidase (GOx) immobilized poly-L-lysine shell. During GOx-catalyzed reaction with glucose, the nanosensor gives rise of a decrease in pH-sensitive fluorescence due to acidification and an increase in O 2 -sensitive fluorescence owing to oxygen depletion, enabling a dual-mode readout under single-excitation wavelength. The emission ratio between O 2 -sensitive peak and pH-sensitive peak, employs signal amplification and results in high sensitivity, compared to single O 2 and pH testing. The ratiometric fluorescent signal exhibits a strong linear response in the 0–1 mM glucose range with a detection limit of 7.3 µM. The sensor shows robust performance and high reproducibility in DMEM and human sweat. In vivo imaging of zebrafish further confirms the photostability, and sensitivity of nanosensor, highlighting their utility for glucose detection. This study presents the first ratiometric fluorescence amplification strategy employing dual pH/O 2 testing for real-time glucose detection. The proposed nanosensor promotes non-invasive glucose detection in biomedical research and dual-mode ratiometric amplification strategies for monitoring metabolic targets. Graphical abstract
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JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 12.6
Ce Li,Yichao Yuan,Yaqing Bao,Yeling Ni,Nan Chen,Jigang Piao,Lu Wang,Zhihong Zhu
Idiopathic pulmonary fibrosis (IPF), a chronic interstitial lung disease, is characterized by progressive fibrosis and poor prognosis, with no current therapies capable of reversing the fibrotic changes. The aberrant repair driven by fibroblast activation and an inflammatory microenvironment results in irreversible IPF. In this work, a macrophage-derived apoptotic body delivery system (SA + NAC@AB) co-loaded with sodium arsenite (SA) and N-acetylcysteine (NAC) was developed to exert synergistic antifibrotic activity against IPF via coordinated regulation of TGF-β1 signaling and inflammation. Apoptotic bodies derived from macrophages inherit inflammation-homing capability, enabling targeted delivery to fibrotic lesions. In vivo evaluation in a bleomycin-induced IPF mouse model demonstrated that SA + NAC@AB effectively targeted the lungs, significantly improved body weight and survival, and alleviated pulmonary fibrosis. Immunofluorescence and Western blot analyzes revealed that SA + NAC@AB reduced Smad2/3 phosphorylation and M2 macrophage polarization, indicating regulation of the TGF-β1/Smad2/3 pathway and inflammation as part of its mechanism of action. Furthermore, in vitro studies validated the enhanced efficacy of SA + NAC@AB, which significantly promoted fibroblast uptake, thereby potentiating its inhibitory effects on fibroblast viability, as well as TGF-β1-induced migration and differentiation. In conclusion, our study demonstrates that SA + NAC@AB represents an effective therapeutic strategy for IPF, offering a promising novel approach by modulating both the TGF-β1/Smad2/3 signaling pathway and the inflammatory response.
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