DALTON TRANSACTIONS
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2026
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IF 3.3
Guorui Song,Jinke Ji,Changying Li,Jinlong Zhao,Bo Zhang
Photodynamic therapy (PDT) has shown good therapeutic results in recent years, but the efficacy is limited by the hypoxic environment within cancer cells. At present, most of the research studies on overcoming PDT hypoxia focus on providing exogenous oxygen, but the effect is not outstanding. This work shows an unconventional source of free radicals, compared with the traditional PDT strategy of generating reactive oxygen species, using a radical polymerization initiator (2,2′-azobis (2,4-dimethylvaleronitrile), ABVN) to generate alkyl radicals, which is free from the limitation of oxygen, is simpler and more efficient, and has obvious therapeutic effects. By synthesizing hollow mesoporous manganese dioxide (MnO2) as a carrier, the nanoparticles are loaded with ABVN and encapsulated with PEG, which have excellent photothermal properties, can quickly heat up to the thermal decomposition temperature of ABVN under laser irradiation, can degrade and release ABVN in GSH and acidic environments, and generate a large number of alkyl radicals in a short period of time, with excellent treatment efficiency. This study breaks through the limitation of PDT caused by hypoxia in cancer cells and provides a promising research strategy for PDT treatment.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
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IF 6.7
Zhuoda Liu,Yifei Zhou,Dongliang Wang,Ruichi Li,Zhen Tian,Yifan Li,Jiyuan Liu
The rapid evolution of insecticide resistance threatens global food security, with cytochrome P450-mediated detoxification representing a key adaptive mechanism in insects. However, direct biochemical evidence linking specific P450s to pyrethroid metabolism in Plutella xylostella remains limited. Here, we investigated the molecular basis of high-level pyrethroid resistance in P. xylostella. Comparative transcriptomic analyses of a susceptible strain and two highly resistant field populations (resistance ratios >650-fold) identified PxCYP6B4 as the most strongly overexpressed P450 (>95-fold). RNA interference-mediated knockdown of PxCYP6B4 significantly increased larval susceptibility to pyrethroids, while heterologous expression in Drosophila melanogaster conferred a 5.27-fold increase in tolerance to lambda-cyhalothrin. Importantly, in vitro assays using recombinant PxCYP6B4 expressed in Sf9 cells, combined with LC–MS/MS analysis, demonstrated direct hydroxylation of lambda-cyhalothrin to 4′-hydroxy-lambda-cyhalothrin. These results establish a direct molecular and biochemical link between PxCYP6B4 overexpression and pyrethroid detoxification.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2026
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IF 3.9
Zheng Zhang,Dan Sui,Ting Xue,Man Xu,Lingling Yang,Kangjun Wang
Lanthanide (Ln)-doped upconversion nanoparticles (UCNPs) exhibit exceptional properties, are usually used to combine multiple components to achieve double fluorescent imaging and photothermal therapy, and have intrigued researchers for several years. Here, Au nanocrystals are successfully combined with UCNPs using a one-pot strategy to form a multifunctional prism-like nanoparticle (UCNP/Au NPs) with adjustable length. Despite Au being only 4% (w/w), a wide near-infrared (NIR) absorption range covers the full NIR-I and NIR-II regions and achieves a photothermal conversion efficiency (η) of up to 35.65%. Significantly, UCNP/Au NPs with varying Au contents exhibited different capabilities in NIR-triggered photothermal conversion and photoluminescence. With the increase of Au contents, the emission wavelengths of green fluorescence have remained almost untouched. Besides, UCNP/Au NPs exhibit satisfactory drug loading efficiency and cumulative release. The study proposes a concise strategy for solutions to many key issues in medical sciences.
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FASEB JOURNAL
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2026
·Vol. 40
·期 3
·e71499
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IF 4.2
Liwen Xu,Fei Zheng,Dandan Wang,Qing Yang
N-terminal acetyltransferases are emerging as potential therapeutic targets in cancer. N-alpha-acetyltransferase 30 (NAA30), which serves as the catalytic subunit of the NATC complex. However, the role of NAA30 in ovarian cancer remains unknown. In this study, we found that NAA30 expression was abnormally upregulated in ovarian cancer tissues compared to normal tissues. Functionally, NAA30 promoted cell proliferation, migration, and invasion in ovarian cancer cells. Moreover, in vivo experiments revealed that NAA30 enhanced tumor growth and intraperitoneal metastasis in mouse models. We further explored the regulatory mechanisms underlying NAA30 upregulation. Dual-luciferase assays demonstrated that the transcription factor nuclear receptor subfamily 2 group C member 2 (NR2C2) significantly enhanced the transcriptional activity of the NAA30 promoter. Besides, NR2C2 increased the migratory, invasive, and proliferative capabilities of ovarian cancer cells. Importantly, NAA30 knockdown reversed the pro-tumorigenic effects of NR2C2 overexpression on the malignant phenotype. To identify the downstream targets of NAA30, we employed IP-LC/MS and N-terminal acetylation modification omics. Actin-Related Protein 2/3 Complex Subunit 1B (ARPC1B) was identified as a direct target of NAA30. It was demonstrated that NAA30 protein binds to ARPC1B protein and that NAA30 knockdown enhanced the polyubiquitination of ARPC1B and promotes its degradation. Crucially, the re-expression of ARPC1B in NAA30-silenced cells effectively restored these malignant phenotypes. These findings highlight the critical role of the NR2C2-NAA30-ARPC1B axis in ovarian cancer progression and provide more foundation for the development of more effective treatment strategies for patients with ovarian cancer. Graphical In ovarian cancer, transcription factor NR2C2 is highly expressed and enhances the transcription of NAA30. This subsequently suppresses the ubiquitination of ARPC1B, leading to its protein stabilization, which ultimately promotes malignant tumor behaviors and disease progression.
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EUROPEAN JOURNAL OF NUTRITION
·
2026
·Vol. 65
·期 2
·52
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IF 4.5
Liu Kaiyue,Li Na,Fan Jiayi,He Siliang,Tang Weixiang,Cao Yaxin,Shen Liang,Sun Shuyang,Du Chengcheng,Du Chao
Purpose This study investigated the basic composition, in vitro digestibility, biological activities, and potential bioactive peptides of Pacific oyster (PO), Mytilus edulis (ME), Argopecten irradians (AIS), and Meretrix meretri x L. (MML) after simulated gastrointestinal digestion (SGD). Methods SGD products were prepared using the INFOGEST 2.0 model, and their nutritional properties and biological activities were analyzed. A comprehensive peptidomic profiling of the digested samples was conducted using nano-liquid chromatography-tandem mass spectrometry (nano-LC MS/MS) analysis. Results All four shellfish exhibited a nutritional profile characterized by high protein and low-fat content. The digestibility of shellfish increased progressively, while the average molecular weight (MW) of the hydrolysates gradually decreased during SGD, with the intestinal hydrolysates showing the highest overall digestibility. Biological activity analysis demonstrated that intestinal digestion products from all four species exhibited notable ABTS radical scavenging activity and α-glucosidase inhibition activity, with PO and ME exhibiting the strongest effects. A total of 349, 99, 314 and 105peptides were identified from the digestion products of PO, ME, AIS, and MML, respectively. Among these, PO and AIS showed the highest sequence similarity, sharing 69 identical peptides. By contrast, MML displayed distinct peptidomic characteristics, including a higher proportion of large MW peptides. Conclusion This study provides a comprehensive peptide catalog of shellfish digestion products and offers new insights into the biological activities of shellfish-derived peptides, supporting their potential as functional food resources.
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Research
·
2026
·
IF 12.9
Yingying Zhang,Shaoqing Chen,Chun Liu,Pengyin Li,Yutao Zhang,Ziman Li,Xinye Ni
Radiotherapy (RT) and photodynamic therapy (PDT) for breast cancer are limited by tumor hypoxia and suboptimal photosensitizer performance. We developed folate-modified copper-doped carbon dots and loaded them with 5-aminolevulinic acid (ALA) to yield FCA, a nanoplatform that executes cascade nanozyme activities to remodel the tumor microenvironment: decomposing H 2 O 2 to relieve hypoxia, generating hydroxyl radicals and singlet oxygen ( 1 O 2 ), and depleting glutathione (GSH). This priming enabled efficient ALA-to-protoporphyrin IX conversion, which subsequently amplified reactive oxygen species generation. The elevated oxidative stress then synergized with RT to accumulate DNA double-strand breaks and trigger cell cycle arrest. Consequently, FCA-PDT-RT reduced 4T1 cell viability to 20.09% and induced 83.82% apoptosis outcomes mechanistically linked to nuclear factor erythroid 2-related factor 2 (NRF2)–Kelch-like ECH-associated protein 1 (KEAP1)–heme oxygenase 1 (HMOX1) pathway activation. Despite compensatory upregulation of antioxidant genes (HMOX1 and glutamate–cysteine ligase modifier subunit [GCLM]), intracellular GSH and adenosine triphosphate were severely depleted, establishing a metabolic crisis wherein synthesis could not match consumption. This redox/energy collapse drove the pronounced cytotoxicity observed. In an orthotopic 4T1 model, FCA-PDT-RT achieved superior tumor control at only 12 Gy, which correlated with increased CD3 + /CD8 + T cell infiltration and suppressed angiogenesis, while maintaining favorable safety. FCA thus enables synergistic PDT-RT through sequential microenvironment remodeling, oxidative amplification, and metabolic exhaustion, offering a dose-sparing strategy with translational promise for breast cancer therapy.
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Foods
·
2026
·Vol. 15
·期 3
·556
·
IF 6
Zhenjing Huang,Jiahao Luo,Xiaoyun Fei,Deming Gong,Xing Hu,Guowen Zhang
Covalent modification of polyphenols effectively enhances functional properties of proteins. This study conjugated potato protein isolate (PPI) with gallic acid (GA) via an alkaline method to investigate structural and functional alterations. Successful conjugation was confirmed by a significant decrease in free amino and sulfhydryl groups, coupled with a marked increase in total phenolic content. Multispectroscopic analyses indicated a loosening of the secondary structure and notable changes in the tertiary conformation. Molecular dynamics (MD) simulations corroborated these findings, revealing that GA conjugation induced conformational expansion, improved structural stability, and enhanced surface hydrophilicity. These structural modifications led to substantial functional improvements in the PPI-GA conjugates, including enhanced dispersion stability, improved emulsifying performance, strengthened antioxidant activity, and increased thermal stability. This research may provide effective strategy and technical support for the improvement of functional properties of PPI and expand its application in the food industry.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
·
IF 8.7
Shuanglong Tang,Xi Wang,Jingyu Li,Xiaodan Wen,Hao Chen,Mei Yan,Xi Chen,Chongshen Guo
Although photothermal therapy (PTT) has been deemed as an effective approach for tumor ablation, the ubiquitous overheat over 50 °C in the PTT process would damage surrounding normal tissue. The combination of nutrient depletion at the tumor site with mild PTT seems like an efficient way to overcome this dilemma. In this work, cobalt-doped polydopamine (CP) behaved as both photothermal agent and glucose oxidase (GOx) carrier to achieve a starvation assisted photothermal therapy. CP possesses more weak acid sites than pure polydopamine, and it gives higher encapsulation efficiency and loading capacity of 89.71% and 47.29% towards GOx, respectively. The CPGP was prepared by coating the GOx-loaded CP with a phase change material (PCM) layer. The solidified PCM prevents the binding between the GOx and glucose, thereby inhibiting GOx activity. The PCM melts at 45 °C and then the GOx activity restores to normal level. The CPGP could cause reduction of intracellular levels of NAD + , NADH and ATP, as well as mitochondrial dysfunction and the induction of apoptosis or necrosis. Hence, CPGP-mediated starvation-photothermal combination therapy exhibits an effective tumor-killing effect. In addition, upregulated M1-polarized macrophages, CD4 + T cells and CD8 + T cells were observed in the mouse model.
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MATERIALS & DESIGN
·
2026
·
IF 8.2
Dashuai Li,Zijian Lin,Wanyi Zhao,Liqun Li,Pan Zhang
Diabetic chronic wounds (DCWs) remain a severe clinical challenge due to their prolonged inflammatory response, impaired tissue repair, high risk of bacterial infection, and limited angiogenesis. To address this, we developed a multifunctional dressing, HA-RHC-AP@MSC-Exo, integrating hyaluronic acid, recombinant human collagen, silver-containing apatite, and mesenchymal stem cell-derived exosomes (MSC-Exo). The dressing exhibits optimal hydrophilicity and degradation rates matching tissue repair. In vitro , it demonstrated potent antibacterial activity against S. aureus and E. coli , augmented by photothermal effects under NIR irradiation. Furthermore, HA-RHC-AP@MSC-Exo promoted the transition of macrophages from pro-inflammatory M1 to anti-inflammatory M2 phenotypes and enhanced fibroblast migration and angiogenesis. In vivo studies in diabetic rat confirmed accelerated wound closure with nearly complete healing by day 15. Histological and transcriptomic analyses revealed enhanced collagen deposition, vascularization, and regulation of key signaling pathways involved in tissue remodeling. Consequently, HA-RHC-AP@MSC-Exo offers a synergistic therapeutic strategy for effective DCW treatment
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Shijing Dong,Liping Guo,Chenyang Xuan,Chenhui Zhu,Zongze Han,Nian Chen,Xinyu Wang,Meng Shen,Xiaoxuan Wei,Hui Yang,Xue Zhang,Man Liu,Simin Zhou,Lu Zhou
Lactobacillus plantarum (L. plantarum ) has shown protective effects in various liver diseases, yet its therapeutic potential and mechanisms in intrahepatic cholestasis remain unelucidated. This study aimed to investigate the therapeutic efficacy and mechanism of L. plantarum in intrahepatic cholestasis using two well-established mouse models induced by α-naphthylisothiocyanate (ANIT) and 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC). The results indicated that L. plantarum significantly alleviated cholestasis-related hepatic inflammation, ductular proliferation, fibrosis, and improved gut barrier integrity. The 16S rRNA sequencing and untargeted metabolomics analysis revealed that the intestinal abundance of L. plantarum was significantly increased after intervention and showed a positive correlation with the level of indole-3-acetic acid (IAA), a key functional metabolite of tryptophan ( r = 0.712, p = 0.003). The elevated IAA levels were closely associated with the improvement of key liver injury indicators. Exogenous IAA supplementation replicated the hepatoprotective effects of L. plantarum in intrahepatic cholestasis. Transcriptome analysis indicated that IAA mediated these benefits primarily through aryl hydrocarbon receptor (AHR) activation, and AHR inhibition markedly attenuated its protective effects. This study reveals that L. plantarum ameliorates intrahepatic cholestasis by enhancing tryptophan metabolism to promote IAA production, which in turn activates the IAA-AHR axis, providing new insights into probiotic and metabolite-based therapies for intrahepatic cholestasis.
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INDUSTRIAL CROPS AND PRODUCTS
·
2026
·
IF 6.4
Penghua Shu,Wenhao Zou,Yu Meng,Jiaqi He,Tingting Guo,Xinfeng Fan,Di Hu,Hao Yin,Shuxian Fan,Na Wang,Simin Liu,Lu Lv,Si Chen,Xialan Wei,Jihong Huang
Astragali complanati Semen (ACS) is frequently used as Chinese medicines and functional foods. Complanatoside A (CA), a key bioactive phytochemical isolated from ACS, exhibits broad-spectrum pharmacological properties. This investigation systematically formulated sixteen natural deep eutectic solvent (NADES) systems by combining three hydrogen-bond acceptors with eleven hydrogen-bond donors at varying molar ratios. These NADES formulations were subsequently employed in tandem with ultrasonic-assisted extraction to isolate CA. Optimization studies identified the following parameters as most effective for extraction using the L -proline/levulinic acid-based NADES (molar ratio 1:2): 60℃, 30 min, 40 % water content, liquid-solid ratio 25 mL/g, achieving an extraction yield of 1.57 %. The adsorption and desorption conditions were optimized employing a macroporous resin for the recovery of CA, while the reusability of the NADES solvent was thoroughly evaluated throughout the extraction process. The CA extract exhibited significant tyrosinase and α-glucosidase inhibitory activity. These findings establish ultrasound-assisted NADES extraction as a sustainable methodology for efficient extraction of CA from ACS, providing valuable insights for eco-friendly extraction and pharmacological exploitation of plant-derived bioactives.
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JOURNAL OF MOLECULAR STRUCTURE
·
2026
·
IF 4.9
Na Yao,Lian Deng,Ziqing Weng,Yang Song,Ning Lei,Junda Liu,Neng Qiu
Podophyllotoxin (PPT) is a natural lignan antitumor compound, but its clinical application is significantly limited by poor water solubility and lack of selectivity. To address these issues, PPT was conjugated with a tumor-targeting ligand, biotin (Bio), through different linkers to prepare three PPT-Bio conjugates: podophyllotoxin-biotin (PB), podophyllotoxin-6-aminohexanoic acid-biotin (PACAB), and podophyllotoxin-3,3’-dithiobispropionic acid-ethanolamine-biotin (PSSB). These conjugates were subsequently complexed with HP-β-CD to form the corresponding inclusion complexes, aiming to improve both tumor-targeting capability and water solubility of PPT. Among them, the PSSB/HP-β-CD complex exhibited 5.68-fold increase in solubility compared to free PPT. In vitro release studies demonstrated that the PSSB/HP-β-CD inclusion complex exhibited an accelerated release under both acidic and reductive environment. Cellular uptake studies revealed significantly higher internalization of all three inclusion complexes in Hela cells compared to free PPT. The targeting specificity of biotin was confirmed by competitive inhibition of the biotin receptor (BR). Further endocytosis pathway studies showed that the internalization process was energy-dependent and involved multiple endocytic pathways. Cytotoxicity assays indicated that all compounds and complexes exhibited concentration-dependent cytotoxicity, with the three inclusion complexes showing significantly stronger antitumor activity than free PPT. Notably, the PSSB/HP-β-CD complex demonstrated the highest potency (IC50 =17.03μM). Additionally, these complexes exhibited low toxicity on normal cells and good hemocompatibility. Preliminary pharmacokinetic evaluation indicated favorable tissue distribution of the biotinylated prodrug conjugates. Therefore, the biotinylated prodrug inclusion complexes, particularly PSSB/HP‑β‑CD, demonstrate great potential as targeted antitumor agents with improved solubility and efficacy.
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