Nano Research
·
2016
·Vol. 9
·期 12
·3683-3694
·
IF 8.893
Deng Jun,Zheng Honghao,Zheng Xiaowen,Yao Mengyun,Li Zheng,Gao Changyou
Chirality is one of the most distinctive biochemical signatures of life, and plays crucial roles in maintaining normal functions of living cells or organisms. Pioneering work from another group has demonstrated the dependency of cell differentiation on the chirality of nano-coated substrates, but the effect of the chiral surface of nanoparticles on stem cell fates has not been investigated. In this study, the influence of molecular chiral poly(acryloyl-L(D)-valine) (L(D)- PAV)-anchored gold nanoparticles (L(D)-PAV-AuNPs) on the differentiation of mesenchymal stem cells (MSCs) was investigated. Though osteogenic differentiation of MSCs was not affected by D-PAV-AuNPs, it was significantly promoted by L-PAV-AuNPs in terms of calcium deposition, alkaline phosphatase (ALP) activity, and expression of collagen type I and osteocalcin (OCN) at both mRNA and protein levels. L-PAV-AuNPs could activate the P38 mitogen-activated protein kinase (MAPK) pathway, and may exert mechanical stress on MSCs because of high amounts of internalization. These results provide new insights on surface chirality at the nanoscale as a direct regulator to guide the differentiation of MSCs, and the use of these nanomaterials for strategic regenerative medicine.
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高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2020
·Vol. 18
·期 1
·1-11
·
IF 6.518
Ji De,Wang Qiaohan,Zhao Qi,Tong Huangjin,Yu Mengting,Wang Meng,Lu Tulin,Jiang Chengxi
Hepatic stellate cells (HSCs) were activated and secreted excessive amounts of extracellular matrix (ECM) proteins during pathogenetic progress of liver fibrosis. Germacrone (GMO) and miR-29b can play an important role in inhibiting growth of HSCs and production of type I collagen. GMO and miR-29b were co-encapsulated into nanoparticles (NPs) based on poly(ethylene glycol)-block-poly(lactide-co-glycolide) (PEG-PLGA). Then, NPs were modified with cyclic RGD peptides (cRGDfK). cRGDfK is an effective ligand to bind integrin αvβ3 and increase the targeting ability for fibrotic liver. GMO- and miR-29b-loaded NPs exhibited great cytotoxicity to activated HSCs and significantly inhibited production of type I collagen. Liver fibrosis model of mice was induced by administration of carbon tetrachloride. Great targeting ability was achieved in liver fibrotic mice treated with cRGD-modified NPs. Significant ant-fibrotic effects have been presented based on hematoxylin and eosin (H&E), Masson and Sirius Red staining results of liver tissues collected from mice treated with drug-loaded NPs. All these results indicate GMO- and miR-29b-loaded cRGD-modified NPs have the potential for clinical use to treat liver fibrosis.
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高影响因子
POLYMER BULLETIN
·
2018
·Vol. 76
·期 8
·4237-4257
·
IF 1.589
Liu Benxing,Tan Lianjiang,He Changyu,Liu Bingya,Zhu Zhenggang,Gong Bing,Shen Yu-Mei
Novel redox-responsive amphiphilic cationic multi-block copolymers PEG 2000 – PLA 3000 – PEI 1200 – PLA 3000 – PEG 2000 and PEG 2000 – PLA 3000 – PEI 1800 – PLA 3000 – PEG 2000 were synthesized and self-assembled into micelles for co-delivery of siRNA and hydrophobic doxorubicin (DOX). The chemical structure and molecular weight of the copolymers were characterized by 1 H nuclear magnetic resonance and gel permeation chromatography, respectively. The copolymeric micelles were examined by dynamic light scattering, and their size, zeta potential and critical micelle concentration were determined. The in vitro drug release analyses indicated that reductive environment can trigger the release of DOX and siRNA by breaking the micelles. MTT assay demonstrated that the DOX/siRNA-loaded micelles are capable of inhibiting proliferation of SGC7901 cells. The results of fluorescence microscopy and flow cytometry verify the simultaneous delivery of DOX and siRNA from the nanomicellar particles into SGC7901 cells. The reduction-responsive cationic copolymers will provide a platform for constructing drug/gene delivery system toward cancer therapy.
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BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2018
·Vol. 101
·期 4
·536-542
·
IF 1.48
Qian Haifeng,Ke Mingjing,Qu Qian,Li Xingxing,Du Benben,Lu Tao,Sun Liwei,Pan Xiangliang
The manufacturers of single-walled carbon nanotubes (SWCNTs) are continuously expanding their manufacturing and commercial markets, indicating that the environmental release and accumulation of SWCNTs in soil is inevitable. However, little is known about the effects of SWCNTs on soil physicochemical properties and soil microbial communities. Our results showed that treatment with SWCNTs resulted in an enhancement of microorganism metabolism related to soil organic compound degradation and a change in the structure of soil microbial communities, but the diversity of soil microorganisms was not significantly affected. The decrease in soil urease activity and the increase in the relative abundance of Nitrospirae after SWCNTs exposure might be relevant to the induction of soil nitrification. The relative abundances of phosphate-solubilizing microorganisms increased after exposure to SWCNTs, which was beneficial for phosphorus bioavailability in the soil. Our current study highlights that exposure to SWCNTs at concentrations of 3 and 10 µg/g can change the composition of soil microorganism communities, promote soil organic degradation and improve soil fertility by enhancing N and P availability in a short time.
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JOURNAL OF PLANT GROWTH REGULATION
·
2018
·Vol. 38
·期 2
·701-712
·
IF 2.047
Liu Shaojin,He Yan,Tian Hao,Yu Chunxin,Tan Weiming,Li Zhaohu,Duan Liusheng
Pesticide residues, especially for herbicides, often damage crops, except for weeds, which results in reduced production or even death in agriculture. Brassinosteroids (BRs) can alleviate the injury from pesticide stress and enhance the growth of plants. However, the roles of low-cost BR mimetics in alleviation and protection from herbicide (nicosulfuron, NSF) stress in maize remain unclear. To investigate the effects of brassinosteroid mimetics on the growth of NSF-stressed plants, we treated maize seedlings with bikinin and brazide at 10 µM prior to NSF treatment, and epibrassinolide (EBL) as a positive control. The NSF treatment dramatically reduced the height, root length, and biomass of maize, and significantly influenced photosynthetic activity and pigments. Accumulation of reactive oxygen species (ROS) and membrane lipid peroxidation, enhanced activity of antioxidant, and detoxification-related enzymes were also observed under NSF stress. As compared to NSF-induced plants, foliar application of bikinin and brazide significantly increased biomass, photosynthesis, and antioxidant enzymes activities and decreased the ROS levels by more than 32.3%, the similar effects as EBL. The glutathione content genes ( GST1, ABC-2, ALS1) involved in detoxification in the BR mimetics + NSF-treated plants were higher than those of NSF alone. Reduced levels of NSF residues by more than 55% after 3 days were observed as a result of BR mimetics pretreatment. In summary, our results present a new pattern of roles of BR mimetics, which display the potential protection of plants under pesticide stress.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
·
2019
·Vol. 30
·期 8
·1-14
·
IF 2.467
Wang Jun,Li Shunying,Chen Tingting,Xian Wenjiao,Zhang Huiwu,Wu Lei,Zhu Wenting,Zeng Qingbing
To enhance the bioavailability of protein therapeutants and improve the stability of storage and delivery, a series of branched amphiphilic block copolymers consisting of cholic acid (CA) initiated poly(D,L-lactide-co-glycolide) (CA-PLGA) and water-soluble polyethyleneimine cross-linked polyethylene glycol (PEI-PEG) denoted as CA-PLGA-b-(PEI-PEG) were synthesized and characterized. CA-PLGA-b-(PEI-PEG) presented low cytotoxicity by MTT and cck-8 assay. The cationic CA-PLGA-b-(PEI-PEG) micelles (diameter about 100 nm and zeta potential 34–61 mV) were prepared through self-assembly method, and complexed with insulin via electrostatic interaction to obtain nanoscale micelle/insulin complexes. The micelle/insulin complexes-loaded CA-PLGA microspheres (MIC-MS, 10.4 ± 3.85 μm) were manufactured by employing a double emulsion (W 1 /O/W 2 ) method. The in vitro insulin release behavior and in vivo hypoglycaemic effect of MIC-MS on streptozotocin (STZ) induced diabetic rats were compared with those of the insulin-loaded CA-PLGA microspheres (INS-MS, 7.8 ± 2.57 μm). The initial burst in vitro release of MIC-MS was markedly lower than that of INS-MS ( P < 0.01), and the pharmacological availability of MIC-MS via subcutaneous administration was 148.9% relative to INS-MS. Therefore, the cationic CA-PLGA-b-(PEI-PEG) micelles can effectively increase the bioavailability of insulin in CA-PLGA microspheres and can be considered as a potential protein carrier.
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Scientific Reports
·
2025
·
IF 3.9
Li Cheng,Jiang Peng,Fan Chenglong,Chen Jinji,Liang Shengsheng,Chen Shaohua,Mi Hua
Ketamine-induced cystitis (KC) manifests as lower urinary tract symptoms stemming from prolonged ketamine abuse, yet its precise pathogenesis remains unclear. It is widely recognized that gut microbiota dysregulation can trigger metabolic aberrations in many diseases. This study aimed to address the dearth of knowledge regarding the functional characteristics of gut microbiota and their metabolites in KC, and to explore the underlying mechanisms of KC from the perspective of the gut-bladder axis. Metagenomic and untargeted metabolomic analyses were employed to elucidate critical features of gut microbiota and metabolism in KC rats. Metagenomic sequencing revealed significant gut microbiota dysregulation, characterized by discrepancies in 46 bacterial taxa at the species level, including Bifidobacterium pseudolongum , Erysipelotrichaceae bacterium OPF54 , Firmicutes bacterium CAG: 424 , and Phocaeicola sartorii . Untargeted metabolomics identified 13 dysregulated metabolites, encompassing Stachydrine, Quinoline, Sedanolide, and others. Correlation analyses among differential gut microbiota, metabolites, and bladder inflammatory factors in KC rats suggested a potential interconnectivity between these factors. Furthermore, the anti-inflammatory property of Stachydrine was experimentally validated using an in vitro model. These findings collectively indicate that KC rats exhibit alterations in gut microbiota composition and metabolites profiles, establishing a preliminary association among gut microbiota, metabolites, and KC pathogenesis. Finally, validation of the anti-inflammatory effects of Stachydrine provides insight into a potential pathogenic pathway involving gut–bladder axis crosstalk, in which dysregulation of gut microbiota and metabolites contributes to the development of KC.
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高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2025
·Vol. 116
·期 1
·10
·
IF 2.2
Huang Junyun,Liu Boying,Yuan Sijia,Zhou Haiyun,Bao Jian,Tu Yong,Wang Zunyao,Wang Weixia
The UV filter benzophenone-3 (BP-3) is a prevalent environmental pollutant due to its extensive use in personal care products, raising concerns about its ecological risks. This study investigated the pH-dependent oxidative stress effects of benzophenone-3 (BP-3) on Carassius auratus by measuring four key biomarkers—superoxide dismutase, catalase, glutathione, and malonaldehyde in liver tissue. The results demonstrated that BP-3 exposure led to significant oxidative stress, as evidenced by altered antioxidant levels and increased lipid peroxidation. The integrated biomarker response indicated that stress was more pronounced at higher BP-3 concentrations and varied with pH, being greatest under acidic conditions. These findings demonstrate that the toxicity of BP-3 is strongly influenced by environmental pH, highlighting the necessity of incorporating pH variations into ecological risk assessments of ionizable organic pollutants.
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MICROCHIMICA ACTA
·
2025
·Vol. 193
·期 1
·49
·
IF 5.3
Wang Chuang,Peng Yue,Miao Yuanyuan,Sun Xinyuan,Cheng Wenting,Li Jinlong
DNA methyltransferases (MTases) play a critical role in epigenetic regulation and are closely associated with the occurrence and development of various diseases. To address the limitations of current detection methods, which often suffer from complicated operation and limited sensitivity we have developed a label-free fluorescent biosensing platform based on DNA-templated silver nanoclusters (DNA-AgNCs) for the detection of DNA adenine methyltransferase (Dam MTase) activity and the screening of its inhibitors. In this system, DNA-AgNCs signal probes are synthesized via a simple reduction reaction. Dumbbell-shaped DNA substrates are designed to be specifically methylated by Dam MTase in the presence of S-adenosylmethionine (SAM). The methylated substrates are subsequently recognized and cleaved by DpnI, yielding DNA fragments with 3′-OH termini. These termini are extended by terminal deoxynucleotidyl transferase (TdT) in the presence of dGTP, generating G-rich sequences, which hybridize with the DNA-AgNCs and significantly enhance the fluorescence intensity. This cascade reaction enables the sensitive detection of Dam MTase activity. Compared with the other approaches, the proposed method exhibits a wider linear range (0.5–10 U/mL) and a lower detection limit (0.165 U/mL). Furthermore, inhibition studies demonstrate that 5-fluorouracil and gentamicin effectively suppress Dam MTase activity. The method also shows excellent performance in complex biological samples. Overall, this fluorescence-based strategy offers high sensitivity and excellent specificity, demonstrating broad application prospects in epigenetic research and drug discovery. Graphical
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POLYMER BULLETIN
·
2025
·Vol. 83
·期 4
·179
·
IF 4
Shen Xiaoxuan,Chen Longhai,Ma Yichang,Li Ziyi,Liang Bo,Zhang Yue-Fei
Using BASF commercial polyurethane 1185 A (TPU) as the modifying material, polyurethane-modified asphalt in varying dosages was created for this study. The rheological properties, fatigue behavior, temperature sensitivity, chemical composition, and self-healing properties of polyurethane-modified asphalt were all characterized using multi-dimensional characterization techniques, such as the dynamic shear rheometer test (DSR), bending creep stiffness test (BBR), fatigue-healing-fatigue test, Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The findings of the study indicated that adding TPU to asphalt can optimize its low-temperature performance, effectively increase its low-temperature flexibility, and greatly increase its resistance to low-temperature cracking. Excellent interfacial adhesion between the modifier and the base asphalt was found by microscopic morphological investigation. According to the results of the fatigue-healing-fatigue tests, the modified asphalt’s self-healing performance progressively improved as the TPU content rose, peaking at 6% TPU content. When the TPU concentration is 6%, the changed asphalt achieves the best overall modification effect, according to a thorough investigation of all performance metrics. This study’s findings offer a theoretical foundation for enhancing matrix asphalt’s rheological characteristics and self-healing capabilities while also prolonging the pavement’s service life.
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JOURNAL OF POLYMER RESEARCH
·
2025
·Vol. 33
·期 1
·10
·
IF 2.8
Sun Ying-Xiang,Lu Kun-Rong,Ji Ying,Guo An-Bang,Zhang Jin,Zhang Yu-Hang
Solid polymer electrolytes (SPEs) are flexible and exhibit wide application prospects. However, their low ionic conductivity at room temperature largely restricts their practical use in next-generation solid-state lithium batteries. A composite polymer electrolyte (CPE) that combines the advantages of soft chains and rigid particles is constructed to balance the electrochemical properties and mechanical strength. Hydrophobic nano-fumed silica (SiO 2 ) fillers are uniformly distributed in the dense cross-linking -CH 2 -CH 2 O- network, which is composed of polyethylene oxide and tetraglyme that is triggered by ultraviolet irradiation. The increasing amorphous region of the resulting CPE, due to the cross-linking and nanoparticles doping, facilitates an excellent electrochemical property with an ionic conductivity of 0.29 mS·cm − 1 and a lithium transference number of 0.60 at room temperature. Moreover, the compact film with a tensile elongation of 57% can provide close interfacial contact and ensure more security for cells at work. The assembled Li||LiFePO 4 cells can deliver a reversible specific capacity of 160 mAh g − 1 at 0.1 C at room temperature and a capacity retention of 98% after 100 cycles. This study proposes a novel method for creating high-performance composite solid electrolytes at room temperature.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2025
·
IF 4
Yan Qin-Yu,Song Shun-Gang,Zhou Bu-Jie,Hu Jing,Feng Lian-Fang,Gu Xue-Ping,Zhang Cai-Liang
Poly(phenylene oxide) (PPO) exhibits excellent dielectric properties, making it an ideal substrate for high-frequency, high-speed copper-clad laminates. The phenolic hydroxyl group at the end of PPO plays a key role in its reactivity. Accurately quantifying the phenolic hydroxyl content in PPO is essential but challenging. In this study, we proposed a method for measuring the phenolic hydroxyl content of PPO using differential UV absorption spectroscopy. In alkaline solutions, the phenolic hydroxyl in PPO completely ionizes to form phenoxide ions, leading to a significant increase in UV absorbance at approximately 250 and 300 nm. Notably, the differential UV absorbance at approximately 300 nm was directly proportional to the phenolic hydroxyl concentration. Using 2,6-dimethylphenol as a standard, a calibration curve was established to relate the phenolic hydroxyl concentration to differential UV absorbance at approximately 300 nm, providing a precise and straightforward method for phenolic hydroxyl quantification in PPO with distinct advantages over conventional techniques.
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