JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2026
·
IF 2.5
Zhaowei Xue,Mengjiao Wang,Ting Linghu,Xiong Guo,Xuemei Qin,Aiping Li
13 C 16 -palmitate tracer lipidomics was optimized for MPC-5 cells. • FHT reshaped lipid flux in adriamycin-injured podocytes. • Acylcarnitine profiling revealed restored fatty acid oxidation. • Integrated metabolomics linked lipid remodeling to podocyte protection. Lipid metabolism disturbances drive Nephrotic Syndrome (NS) progression. Current NS therapies have limited efficacy against lipid disorders and often cause significant side effects. Previously, our glucose tracer profiling revealed a compensatory activation of fatty acid synthesis in NS, highlighting lipid metabolism as a critical pathophysiological factor. However, the specific molecular targets through which the multi-component Fangji Huangqi Tang (FHT) regulates this process remain undefined. This study evaluated FHT's protective effects using an adriamycin (ADR)-induced MPC-5 podocyte injury model. We integrated an optimized 13 C 16 -palmitic acid tracer platform with acylcarnitine-targeted metabolomics and molecular biology to dissect underlying mechanisms. FHT intervention significantly attenuated podocyte apoptosis and neutral lipid deposition while upregulating structural proteins. Crucially, it rescued mitochondrial dysfunction by restoring membrane potential, ATP levels, and cellular oxygen consumption. Metabolomic analysis demonstrated that FHT rebalanced lipid homeostasis by inhibiting triglyceride synthesis, promoting triglyceride catabolism, enhancing phospholipid synthesis, and restoring fatty acid oxidation. In conclusion, FHT mitigates podocyte injury by reshaping lipid metabolic homeostasis via multiple targets, providing a strong mechanistic basis for its clinical use in NS.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Yaoyu Zhang,Yuxiong Wang,Yue Liu,Huimin Xu,Zhongbiao Wu
Pt@Cu-ZSM-5@CuO x catalyst was constructed for the catalytic oxidation of acetonitrile (AN), which achieved a complete mineralization rate and more than 95 % N 2 selectivity within 300–450 °C. Characterization results revealed that the satisfactory synergistic action of hydrolysis and oxidation pathways was well achieved. The NO x release from CN oxidation reaction on out-layer CuO x species and NH x species over-oxidation over Pt sites could be eliminated by NH 3 from CN hydrolysis reaction on Cu ions via internal selective catalytic reduction mechanism. The deep oxidation of carbon-containing intermediates (such as CO etc.) during CN decomposition over Cu species effectively occurred over Pt site. The encapsulation of Pt inner ZSM-5 zeolite not only strengthened low-temperature mineralization rate compared to Cu/ZSM-5 sample, but also inhibited NO x release caused by over-oxidation of AN at an elevated temperature with co-impregnation of Pt species over Cu/ZSM-5. This work provided a viable strategy for designing catalysts to effective abatement of NVOCs.
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NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 47
·期 5
·2248-2256
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IF 3.925
Defeng Hu,Rongzhong Li,Yide Han,Hao Meng,Xia Zhang
Currently, the co-immobilization of multi-enzymes on a solid carrier and their applications in cascade catalytic reactions are primarily studied. In this study, a MOF/polymer porous membrane composed of DUT-5 incorporated polyvinylidene fluoride (PVDF) is constructed and employed in the co-immobilization of porcine pancreatic lipase (PPL) and glucose oxidase (GOx) for the first time. The loading amounts of DUT-5 nanoparticles were varied to optimize the subsequent immobilization efficiency for the two enzymes. The adsorption conditions, isotherms and kinetics for both PPL and GOx were investigated systematically, and the optimum adsorption conditions were determined and used in the co-immobilization of the two enzymes step by step. It is interesting that the maximum adsorption amounts for the two enzymes in the co-immobilization process are similar to the results obtained in the single enzyme immobilization, which indicates the better compatibility of PPL and GOx in the hybrid membranes. The catalytic activities of the co-immobilized enzymes were evaluated using a cascade oxidation reaction, in which H2O2 produced in the GOx-catalyzed oxidation of glucose worked as the oxidant in the subsequent oxidation of styrene. Further, the catalytic efficiency of the co-immobilized enzymes is compared to that of the mixed free PPL and GOx. The improved catalytic rates and better reusability of the co-immobilized enzymes highlight the broader applications of MOF-based membranes in the co-immobilization of two or more enzymes and the catalytic applicabality in the cascade organic reactions.
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MOLECULAR BIOTECHNOLOGY
·
2023
·
IF 2.6
Liu Hu,Li Tao,Ma Ben,Wang Yue,Sun Jun
Osteoblast regeneration, characterized by osteoblast differentiation, is the basis of fracture healing and accelerates fracture repair. It has been reported that hyaluronan and proteoglycan link protein 1 (HAPLN1) is overexpressed during osteoblast differentiation and regulates cartilage regeneration, but its function in fracture healing remains unclear. To elucidate this issue, we collected clinical blood samples of fracture healing, established a femoral fracture rat model, and induced an osteoblast differentiation cell model. We found that HAPLN1 was overexpressed in the serum of patients with fracture healing and the bone tissues of rats with fracture healing. Furthermore, the expression of HAPLN1 was increased time dependently during the osteogenic differentiation of MC3T3-E1 cells. HAPLN1 silencing prevented osteoblast differentiation and mineralization in MC3T3-E1 cells as evidenced by decreased osteoblast differentiation-related factors, suppressed alkaline phosphatase activities, and reduced alizarin red positive staining. Mechanically, the bone morphogenic protein 4 (BMP4)/Smad1/5/8 pathway, a facilitator of osteoblastic differentiation, was found to be inhibited by HAPLN1 knockdown, and inhibition of BMP4/Smad1/5/8 signaling enhanced the effects caused by HAPLN1 silencing. These findings demonstrated that HAPLN1 might promote fracture healing by facilitating osteogenic differentiation through the BMP4/Smad1/5/8 pathway, indicating that targeting HAPLN1 may be a feasible therapeutic candidate for fracture repair. Graphical Abstract
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TALANTA
·
2023
·
IF 6.1
Dan Luo,Zaiqiong Liu,Aiwen Su,Yanli Zhang,Hongbin Wang,Lijuan Yang,Wenrong Yang,Pengfei Pang
T4 polynucleotide kinase (T4 PNK) is an important DNA repair-related enzyme that plays a crucial role in DNA recombination, replication and damage repair. Herein, an electrochemical biosensor was developed for detection of T4 PNK activity and inhibitor screening based on supramolecular host–guest recognition between phosphate pillar (Dumitrache and McKinnon, 2017) [5] arene (PP5) and methylene blue (MB). The water-soluble PP5 employed as the host for complexation of MB guest molecules. The substrate DNA with 5′-hydroxyl group was first self-assembled on the gold electrode surface through the chemical adsorption of the thiol group , which was phosphorylated in the presence of T4 PNK and adenosine triphosphate (ATP). TiO 2 served as a bridge to link phosphorylated DNA and PP5 via the robust phosphate-Ti 4+ -phosphate chemistry . The immobilized PP5 captured the MB on electrode surface via the supramolecular host–guest recognition interaction, resulting in an enhanced electrochemical response signal. The electrochemical signal is proportional to the T4 PNK concentration in the range of 2 × 10 −4 to 5 U mL −1 with a detection limit of 1 × 10 −4 U mL −1 . It was also successfully used for PNK inhibitor screening and PNK activity assay in HeLa cell lysates sample. The proposed strategy provides a novel sensing platform for kinase activity assay and inhibitor screening, holding a great potential in clinical diagnostics, inhibitor screening, and nucleotide kinase-target drug discovery.
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FUEL
·
2023
·
IF 7.4
Yuting Jin,Yanhong Quan,Yan Li,Jinxian Zhao,Jun Ren
A series of chainlike ZSM-5 supported Cu catalysts with the addition of citric acid (CA) was conveniently prepared using a complexation-impregnation method and employed in the methanol dehydrogenation to methyl formate (MF). The catalytic performance of Cu x /CZ5 first increased and then decreased with increasing CA/Cu ratio, reached 35.0% methanol conversion and 88.3% MF selectivity for Cu1.8/CZ5, significantly higher than that of Cu/CZ5. The superior catalytic performance might be principally explained by the higher Cu 0 concentration combined with abundant Brønsted acid sites, which are responsible for methanol conversion and MF selectivity, respectively. This work provides new insights into the synergy between Cu 0 species and surface acidity of Cu x /CZ5 catalysts for methanol dehydrogenation and offers a feasible and effective strategy for enhancement of the catalytic performance of copper-based catalysts.
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ChemistrySelect
·
2023
·Vol. 8
·期 23
·e202300862
·
IF 2.1
Xiaoyan Chen,Jingjing Wang,Zhao Du,Huayi Cai,Yang Huang,Guifeng Chen,Chengchun Tang,Yi Fang
Graphical The article reports the CuPd/BCN bimetallic catalysts were prepared for the selective hydrogenation reaction of HMF. The 10Cu3Pd/BCN catalyst exhibited extremely strong catalytic activity and favorable recoverability under the combined action of Cu−Pd alloy and support. In the present world, 2,5-dimethylfuran (DMF), as a renewable liquid fuel, is valuable for maintaining ecological stability. Therefore, it is quite necessary to enhance the selectivity of DMF during the hydrodeoxygenation (HDO) of 5-hydroxymethylfurfural (HMF) to DMF. In this paper, a copper-palladium bimetal supported on carbon-doped boron nitride (BCN) catalyst was prepared as a class of catalyst for HDO. Notably, under the combined action of Cu−Pd alloy and support, the catalyst of 10Cu3Pd/BCN showed remarkable activity with high selectivity (99.2 %) for DMF. In addition, the packing and dispersion of the alloy particles by the carrier improved the cycling performance of the catalyst. Therefore, the selection of 10Cu3Pd/BCN as a catalyst for HDO has excellent application prospects.
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JOURNAL OF POROUS MATERIALS
·
2022
·Vol. 29
·期 4
·1039-1047
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IF 2.523
Yang Zhigang,Li Yanru,Wang Dongliang,Long Xuefeng,Li Hongwei,Zhao Xinhong
Currently, synthesizing zeolites with specific physicochemical properties in a more environmentally benign and safe way is challenging. Herein we report a novel ionothermal route of preparing HZSM-5 zeolite, which is featured by the direct interzeolite transformation of HY to HZSM-5. The appropriate synthetic conditions of this HZSM-5 material are refined. Although performing a series of optimization, the resultant product still contains minor (NH 4 ) 3 AlF 6 impurity. Pure HZSM-5 phase with high relative crystallinity can be obtained through successive calcination and acid-washing treatments towards the as-made sample. SEM–EDS characterization and nitrogen physisorption measurement reveal that this pure HZSM-5 sample is composed of classical coffin-like crystals with a low Si/Al ratio and evident hierarchical micro-mesoporous structure. Benefiting from its unique physicochemical properties, this pure HZSM-5 catalyst exhibits more superior methanol-to-aromatics performance than those of the state-of-the-art HZSM-5 and Metal/ZSM-5 catalysts evaluated under similar reaction conditions. It can be anticipated that this HZSM-5 material may be applied in other industrially relevant reactions owing to its particular structure and properties.
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Catalysis Science & Technology
·
2022
·Vol. 12
·期 10
·3363-3371
·
IF 6.177
Gang Xu,Chenyu Chen,Mengxia Li,Xinyi Ren,Lianggao Hu,Chengrong Wu,Yu Zhuang,Fanan Wang
The electrocatalytic HMF oxidation reaction (HMFOR) is one of the promising technologies for the valorization of biomass derivatives and potential replacements for the energy-consuming oxygen evolution reaction (OER). For the most investigated Ni-based electrode, the in situ reconstruction from Ni(OH)2 to NiOOH is hardly avoidable during the electro-oxidation reaction, which is also a highly indispensable prerequisite towards HMFOR. Herein, we developed an efficient strategy of sacrificing W to investigate the effect of surface defects on the self-reconstruction of Ni for promoting HMFOR. It was rationalized that the selective and rapid exsolution of the W species from the as-prepared NiW layer created a porous structure and induced lattice defects on the surface, which facilitated the ion and electron transportation, and further promoted the in situ reconstruction for the formation of higher oxidated Ni with superior activity for HMFOR. This work offers a novel and low-cost means to enrich the active sites for the electrocatalytic upgrading of HMF and other bio-derived platforms.
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Frontiers in Chemistry
·
2022
·
IF 5.545
Wang Huan,Fan Yanmiao,Yang Qiaoli,Sun Xiaoyu,Liu Hao,Chen Wei,Aziz Ayesha,Wang Shenqi
Redox activity is an important indicator for evaluating electrochemical biosensors. In this work, we have successfully polymerized indole-5-carboxylic acid into poly-5-carboxyindole nanomaterials (PI-5-CA), using its superior redox activity, and introduced carboxylated single-walled carbon nanotubes (C-SWCNTs) to synthesize a composite material. Finally, a synthesized composite material was used for the modification of the glass carbon electrode to fabricate the PI-5-CA/C-SWCNTs/GCE-based immunosensor and was successfully applied for the sensitive detection of E. coli O157:H7. The fabricated immunosensor exhibited an outstanding electrocatalytic activity toward the detection of E. coli O157:H7 with a remarkably lowest limit of detection (2.5 CFU/ml, LOD = 3 SD/k, n = 3) and has a wide linear range from 2.98×101 to 2.98×107 CFU/ml. Inspired from the excellent results, the fabricated electrode was applied for the detection of bacteria from real samples (water samples) with a good recovery rate (98.13–107.69%) as well as an excellent stability and specificity. Owing to its simple preparation, excellent performance, and detection time within 30 min, our proposed immunosensor will open a new horizon in different fields for the sensitive detection of bacteria from real samples.
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Juntao Wang,Jiajia Liu,Weihua Liu,Ziwang Liu,Qiuhua Wu,Zhi Wang,Hongyuan Yan
A novel magnetic hyper crosslinked polymer (BTA-MHCP) was prepared by one-pot reaction of benzotriazole, 1,3,5-triphenylbenzene and phenyl-functionalized Fe 3 O 4 @SiO 2 . The BTA-MHCP was then investigated as the adsorbent for the rapid magnetic solid-phase extraction (MSPE) of the 5-nitroimidazoles (5-NDZs) including dimetridazole, metronidazole, secnidazole, ronidazole and ornidazole. The adsorbent showed good adsorption properties for the 5-NDZs. The key factors that affect the MSPE such as extraction time, the amount of sorbent, and the type of eluent and its volume were optimized. Under the optimal conditions, the linear response ranges for the established method were 0.15–100.0 ng mL −1 and 3.9–100.0 ng g −1 for drinking water and fish samples, respectively, with the coefficients of determination ( r 2 ) of 0.9981 to 0.9998. The limits of detection (S/N = 3) for the analytes were 0.02–0.05 ng mL −1 and 0.7–1.3 ng g −1 , and the method recoveries ranged from 92.0% to 101.0% and from 89.9% to 104.0% for drinking water and fish samples, respectively.
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CHEMICO-BIOLOGICAL INTERACTIONS
·
2021
·
IF 5.194
Shuaibing Liu,Ziteng Wang,Eric Chan,Yibo Zhao,Jian Kang,Xiaojian Zhang,Xin Tian
Vicagrel, an antiplatelet drug candidate targeting platelet P2Y 12 receptor and has finished its phase II clinical trial . The inhibition of six major cytochrome P450 enzymes (P450) (CYP2B6, CYP2C8 , CYP2C9 , CYP2C19 , CYP2D6 , and CYP3A4) and six UDP-glucuronosyltransferases (UGT) (UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A9, and UGT2B7) by vicagrel was evaluated using pooled human liver microsomes and specific probe substrates. Physiology-based pharmacokinetic (PBPK) simulation was further applied to predict the in vivo drug-drug interaction (DDI) potential between vicagrel and bupropion as well as S-mephenytoin. The results suggested that vicagrel inhibited CYP2B6 and CYP2C19 potently with apparent IC 50 values of 1.6 and 2.0 μM, respectively. In terms of mode of reversible inhibition, vicagrel exhibited mixed-type inhibition of CYP2B6-catalyzed bupropion hydroxylation and noncompetitive inhibition of CYP2C19-mediated S-mephenytoin 4′-hydroxylation with K i values of 0.19 μM and 1.2 μM, respectively. Vicagrel displayed profound time-dependent inhibition towards CYP2B6 with maximal rate constant of inactivation ( k inact ) and half-maximal inactivator concentration ( K I ) values of 0.062 min −1 and 1.52 μM, respectively. No time-dependent inhibition by vicagrel was noted for CYP2C19. For UGT, negligible to moderate inhibition by vicagrel was observed with IC 50 values of >50.0, >50.0, 28.2, 8.7, >50.0 and 28.2 μM for UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A9 and UGT2B7, respectively. In terms of mode of reversible inhibition, vicagrel exhibited mixed-type inhibition of UGT1A6-catalyzed N-Acetylserotonin β-D-glucuronidation with a K i value of 5.6 μM. No time-dependent inhibition by vicagrel was noted for UGT1A6. PBPK simulation indicated that neither altered AUC nor C max of bupropion and S-mephenytoin was observed in the presence of vicagrel. Our study provides inhibitory constants for future DDI prediction between vicagrel and drug substrates of CYP2B6, CYP2C19 and UGT1A6. In addition, our simulation suggests the lack of clinically important DDI between vicagrel and bupropion or S-mephenytoin.
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