ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2020
·Vol. 28
·期 4
·4262-4275
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IF 3.056
Li Chao,Luo Miaomiao,Zhou Shihua,He Hanyue,Cao Jiashun,Luo Jingyang
l -Threonine and three kinds of conductive polymers were applied for anode modification in microbial fuel cells (MFCs) for decolorization of Congo red with simultaneous electricity generation. The description of modified anodes with FTIR, surface contact angle, and CV analysis showed that the anode surface was successfully grafted with functional groups, with improving wettability, as well as the increasing specific surface area and electrochemical activity. For l -threonine modification, the highest decolorization rate of 97% of the MFC, and meanwhile, the maximum current density of 155.8 mA/m 2 , was obtained at the modified concentration of 400 mg/L. For conductive polymer modifications, the poly (aniline-1,8-diaminonaphthalene) (short for PANDAN) owned the highest performance, with the current density 185 mA/m 2 , and the decolorization rate was 97%. Compared with l -threonine, the modifications by conductive polymers were more suitable for MFC decolorization due to their functional groups and unique conductivity. In addition, high-throughput sequencing analysis was conducted for the conductive polymers modified anodes to reveal their bioelectrochemical mechanisms.
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Food & Function
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2020
·Vol. 11
·期 11
·9535-9546
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IF 4.171
Na Deng,Ruixue Guo,Bisheng Zheng,Tong Li,Rui Hai Liu
The effects of whole grain highland barley (WGH) with rich phenolics on glucose metabolism, the insulin pathway, and microRNA (miRNA) expression in db/db mice were explored in the present study. Supplementation with WGH decreased the levels of blood glucose, glycosylated serum protein (GSP), insulin, and inflammatory cytokines in db/db mice. Furthermore, WGH administration triggered a remarkable amelioration of glucose intolerance and insulin resistance. The hepatic glucose-6-phosphatase (G6Pase) and phosphoenolpyruvate carboxylase (PEPCK) activities and the G6PC, PEPCK, and forkhead transcription factor 1 (FOXO1) mRNA levels in the WGH-treated group were also reduced. Moreover, WGH promoted the glycogen storage in the liver via up-regulating the activities of hexokinase (HK) and glycogen synthase (GS) and the phosphorylation of glycogen synthase kinase 3β (GSK3β) protein, while down-regulating the GSK3β mRNA level. The protein expression of phosphatidylinositol 3-kinase (PI3K), the phosphorylation of protein kinase B (Akt), and the mRNA levels of insulin receptor substrate-1 (IRS-1), PI3K and Akt were also up-regulated by WGH treatment. Moreover, WGH significantly augmented the expression of miRNA-26a and miRNA-451, but reduced those of miRNA-126a and miRNA-29a. These results demonstrated that WGH exhibits a hypoglycemic effect through regulating the IRS-1/PI3K/Akt pathway and related miRNAs, further modulating the expression of G6PC, PEPCK, and FOXO1 mRNAs and p-GSK3β protein, thus inhibiting hepatic gluconeogenesis, improving glycogen synthesis and alleviating insulin resistance. Therefore, this study suggested WGH as an effective candidate to ameliorate the hyperglycemia of type 2 diabetes mellitus.
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BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Guoyan Zhang,Hossain M. Zabed,Junhua Yun,Jiao Yuan,Yufei Zhang,Yang Wang,Xianghui Qi
In this study, a new strain of Lactobacillus plantarum (CY.6) was identified and its L-arabinose isomerase (L-AI) encoding gene ( ara A) was overexpressed in Escherichia coli BL21 for the biosynthesis of D-tagatose from milk whey powders (WP). Whole-cell biotransformation of lactose in WP into D-tagatose was done by three technological approaches, including 100%, 50% and 0% hydrolysis of lactose in WP before biotransformation, where simultaneous saccharification and biotransformation (SSB, 0% prior hydrolysis of lactose) produced maximum amounts of D-tagatose. Two-stage SSB provided 73.6% conversion efficiency (based on D-galactose) and 36.8% (in term of lactose), with 51.5 g/L of D-tagatose after 96 h, while concentration of D-tagatose produced after first stage was 34.4 g/L. Yield and volumetric productivity of D-tagatose after two-stage SSB were found to be 0.26 g/g of WP (0.37 g/g of lactose, 0.74 g/g of D-galactose produced from lactose) and 0.54 g/L/h, respectively.
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Nanoscale
·
2020
·Vol. 12
·期 35
·18225-18239
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IF 6.895
Kun Liu,Ling Zhu,Shengyue Tang,Wei Wen,Lu Lu,Mingxian Liu,Changren Zhou,Binghong Luo
Although poly(L-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair. Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix. Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) composite inks. Then, the CP porous composite scaffolds were further fabricated via the direct ink writing method. The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%. Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs. Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0–20 wt%. However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds. Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties. These robust CP composite scaffolds offer a new route for bone tissue engineering application.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2020
·Vol. 167
·期 8
·086508
·
IF 3.721
Suxia Wang,Min Zhou,Yongxi Zhu,Bo Peng,Jia Yang,Yongjun Ma
In this study, a novel poly(L-citrulline)/lanthanide-containing cyano-bridged heterometallic coordination polymer modified glassy carbon electrode, poly(L-Cit)/(Ho(III)-Fe(III)-WO42− CyHMCP/GCE, was constructed as a sensing platform for the electrochemical detection of 3-nitropropionic acid (3-NPA) using the two-step electrosynthesis method. The surface morphology of materials coated on the chemically modified electrode was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Based on the excellent synergistic electrocatalytic performance for electrochemical reduction of 3-NPA, the voltammetric detection of 3-NPA in real sugarcane juice samples was achieved with a facile sample pretreatment process and less matrix interference. Furthermore, by means of water-phase infrared spectra technique, the electrocatalytic reduction pathway of 3-NPA was preliminarily studied. Under the optimized detection conditions, a good linear relationship was obtained between the reduction peak current of 3-NPA and testing concentration in the range of 40.0–4.00 × 103 μM, with a regression equation of Ip (μA) = −3.126 + 0.08804C3-NPA (μM), R2 = 0.9997. The lower detection limit (LOD) of 3-NPA was estimated to be 16.0 μM (S/N = 3). Therefore, the method had been employed to analyze raw sugarcane samples, with the average recovery of 96.8%–104%, and the actual concentration level of free 3-NPA in the raw sugarcane juices was revealed.
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FOOD CHEMISTRY
·
2020
·
IF 6.306
Xinlei Yu,Yuchuan Li,Chang He,Jingtao Zhou,Yuqiong Chen,Zhi Yu,Pu Wang,Dejiang Ni
The influence mechanism of different withering methods (CK, indoor natural spreading; LTD, low-temperature plus dark; LTY, low-temperature plus yellow-light; LTCD, low-temperature plus CO 2 ) on non-volatile compounds in postharvest tea leaves was investigated by UHPLC-Q‐TOF/MS-based non-targeted metabolomic and transcriptomic analyses. Compared with CK, low-temperature withering could slow down polyphenol oxidation by inhibiting polyphenol oxidase activity and keeping the expression of genes for flavanol synthesis. After withering, the proteinaceous amino acid content increased significantly, especially for LTCD and LTY, mainly due to increased peptidase activity and up-regulation of genes involved in the biosynthesis of valine, leucine, aspartic acid, glutamic acid, phenylalanine, and proline. Moreover, LTCD and LTY enhanced the synthesis of γ-aminobutyric acid and metabolism of phenylalanine-methyl salicylate and tryptophan-indole, respectively. Meanwhile, the transformation of theobromine to caffeine was accelerated under low-temperature withering. This research provides a genetic metabolic basis for the application of low-temperature withering to actual green tea processing.
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ENVIRONMENT INTERNATIONAL
·
2019
·
IF 7.943
Xinying Gong,Yu Wang,Jian Pu,Junjie Zhang,Hongwen Sun,Lei Wang
To reveal the metabolic characteristic of organophosphate esters (OPEs) in plants, uptake efficiency, subcellular distribution and hydrolysis of OPEs and their hydrolysis metabolites in wheat ( Triticum aestivum L.) were investigated by hydroponic experiments. Tris(2-chloroethyl) phosphate (TCEP) and bis(2-chloroethyl) phosphate (BCEP) were prone to transporting to shoots by transpiration stream, with the translocation factors of 6.2 and 21 for TCEP and BCEP, respectively, as greater than 40% of TCEP and BECP were distributed in the cell sap due to their hydrophilicity. Hydrophobic OPEs (i.e. tri- n -butyl phosphate [TnBP] and triphenyl phosphate [TPhP]), and their hydrolysis metabolites (di- n -butyl phosphate [DnBP] and diphenyl phosphate [DPhP]) were stored in roots, resulting in low translocation capacity in wheat. As organophosphate diesters with relatively high proportions in cell walls (70–84% of DnBP and 41–43% of DPhP) are difficult to being transmembrane transported due to electrical repulsion of the cytomembrane, it is supposed that cell walls could be a main location for in vivo hydrolysis of OPEs. For DnBP, absorption by roots after in vitro hydrolysis of TnBP in hydroponic media was also an important source in wheat. Inhibition experiments showed that it is usually a non-energy-consuming process for root uptake of OPEs and their hydrolysis metabolites. The uptake of OPEs (i.e. TCEP, TnBP, and TPhP) and DPhP were facilitated diffusion mediated by aquaporins or anion channels, while uptake of BCEP and DnBP were simple diffusion processes. This study illustrated the distribution characteristics and translocation capacity of OPEs and their diester metabolites at the subcellular level.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Guanjie Liu,Liu Yang,Yifei Tang,Jiacheng Lin,Fang Wang,Jie Shen,Bin Chang,Xiaoni Kong
Ethnopharmacological relevance Traditional Chinese medicine (TCM) holds that non-alcoholic fatty liver disease (NAFLD) belong to the category of “thoracic fullness”. Polygonum perfoliatum L . (PPL), a Chinese medicinal herb with the effect of treating thoracic fullness, was recorded in the ancient Chinese medicine book “Supplements to Compendium of Materia Medica”. It has been used since ancient times to treat NAFLD. However, the underlying mechanism and active components of PPL against NAFLD remains unclear. Aim of study To identify the main active components and the anti-NAFLD mechanism of PPL. Materials and methods Network pharmacology, UPLC/QE-HFX analysis, and molecular docking were employed to determine the main bioactive compounds and key targets of PPL for the NAFLD treatment. This effect was further validated with administration of PPL (200 mg/kg and 400 mg/kg) to NAFLD model mice for 5 weeks. Systemic signs of obesity, biochemical parameters, and histological changes were characterized. Immunohistochemistry, western blot, and PCR analysis were conducted to elucidate the mechanistic pathways through which PPL exerts its effects. Results Network pharmacology revealed 77 crossover genes between the PPL and NAFLD. The kyoto encyclopedia of genes and genomes (KEGG) analysis show that PPL treat NAFLD mainly regulating glucose-lipid metabolism mediated by PI3K/AKT signal pathway. The Gene Ontology (GO) enrichment analysis show that PPL treat NAFLD mainly regulating inflammation mediated by cytokine-mediated signaling pathway. In accordance with the anticipated outcomes, administration of PPL in a dose-dependent manner effectively mitigated insulin resistance induced by a high-fat diet (HFD) by activating the PI3K/AKT signaling pathway. Histopathological evaluation corroborated the hepatoprotective effects of PPL against HFD-induced hepatic steatosis, as evidenced by the inhibition of de novo fatty acid synthesis and promotion of fatty acid β-oxidation (FAO). Further research showed that PPL blocked cytokine production by inhibiting the NF-κB pathway, thereby reducing immune cell infiltration. Furthermore, five flavonoids from PPL, including quercetin, baicalein, galangin, apigenin, and genistein were identified as key compounds based on ingredient-target-pathway network analysis. Molecular docking show that these active compounds have favorable binding interactions with AKT1, PIK3R1, and MAPK1, further confirming the impact of PPL on the PI3K/AKT pathway. Conclusions Through the combination of network pharmacology prediction and experimental validation, this work determined that therapeutic effect of PPL on NAFLD, and such protective effect is mediated by activating PI3K/AKT-mediated glucolipid metabolism pathway and hepatic NF-κB-mediated cytokine signaling pathway.
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FISH & SHELLFISH IMMUNOLOGY
·
2023
·
IF 4.7
Lei Diao,Meiqi Ding,Hongzhen Sun,Yawei Xu,Rui Yin,Huijie Chen
Polystyrene microplastics (PM) is a pressing global environmental concern, posing substantial risks to aquatic ecosystems . Microalgal astaxanthin (MA), a heme pigment, safeguards cells against oxidative damage induced by free radicals, which contributes to various health conditions, including aging, inflammation and chronic diseases. Herein, we investigated the potential of MA in ameliorating the immunotoxicity of PM on carp ( Cyprinus carpio L.) based on head kidney lymphocytes treated with PM (250 μM) and/or MA (100 μM). Firstly, CCK8 results showed that PM resulted in excessive death of head kidney lymphocytes. Secondly, head kidney lymphocytes treated with PM had a higher proportion of necroptosis, and the levels of necroptosis-related genes in head kidney lymphocytes were increased. Thirdly, the relative red fluorescence intensity of JC-1 and MitoSox showed decreased mitochondrial membrane potential and increased mtROS in head kidney lymphocytes treated with PM. MitoTracker® Green FM fluorescence analysis revealed enhanced mitochondrial Ca 2+ levels in PM-treated lymphocytes, corroborating the association between PM exposure and elevated intracellular Ca 2+ dynamics. PM exposure resulted in upregulation of calcium homeostasis-related gene (Orail, CAMKIIδ and SLC8A1) in lymphocytes. Subsequent investigations revealed that PM exposure reduced miR-25-5p expression while increasing levels of MCU, MICU1, and MCUR1 . Notably, these effects were counteracted by treatment with MA. Furthermore, PM led to the elevated secretion of inflammatory factors (IFN-γ, IL-1β, IL-2 and TNF-α), thereby inducing immune dysfunction in head kidney lymphocytes. Encouragingly, MA treatment effectively mitigated the immunotoxic effects induced by PM, demonstrating its potential in ameliorating necroptosis, mitochondrial dysfunction and immune impairment via regulating the miR-25-5p/MCU axis in lymphocytes. This study sheds light on safeguarding farmed fish against agrobiological threats posed by PM, highlighting the valuable applications of MA in aquaculture.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xiao-Jie Yue,Peng-Wei Xu,Yuan Zhu,Shou-Bu Hou,Xiao-Chuan Luo,Bing Zhao
The structural properties and biological activities of okra pectic polysaccharides (OPs) were impacted by various extraction methods. Based on commonly grinding (40, 100 meshes) and superfine grinding okra powders, two extraction solvents (hydrochloric acid, HA; citric acid , CA) were used firstly. Next, the extraction yield, physical and chemical properties, molecular structure and functional properties of OPs were analyzed by non-ultrasonic treatment and ultrasound-assisted superfine grinding method. The outcomes demonstrated that the extraction yield of OPs rose as the particle size of the powder decreased. HA-OPs had higher molecular weight (Mw), apparent viscosity and emulsification ability than CA-OPs. CA-OPs had higher esterification degree (DE), solubility and total sugar content, and higher amounts of rhamnogalacturonan-I (RG-I) segments. Compared with OPs without ultrasound-assisted extraction, ultrasound-assisted superfine grinding extraction exhibited higher sugar content, antioxidant capacity, emulsification ability, lower Mw, DE and apparent viscosity. Finally, the correlation between structure and function of OPs was further quantified. The antioxidant capacity was positively correlated with RG-I content, and negatively correlated with DE and Mw. The emulsification ability was mainly positively correlated with the GlcA of OPs. This study provides a theoretical basis for the development of OPs foods with clear structure-function relationship, which would be instructive for the application of OPs in food and cosmetics.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2023
·
IF 3
Kun Wang,Wei Li,Ying Yu,Hui Li
In this study, dopamine hydrochloride was grafted onto the sulfated carboxymethylcellulose sodium with different sulfated degree (DA-g-SCMC S ) as heparin-like polysaccharides. By studying the preliminary structure and coagulation time of DA-g-SCMC S , the DA-g-SCMC2 possessing good anticoagulant properties were selected to be assembled layer by layer on the surface of aminated poly (L-lactic acid) (APLLA) film with chitosan (CS). The characterization properties including morphologies and wettability of DA-g-SCMC2/CS multilayers modified APLLA films were studied by scanning electron microscope and water contact angle instrument. Anticoagulation tests revealed that the (DA-g-SCMC2/CS) 15 multilayers modified APLLA film experienced a decrease of 37.5% and 73.68% in the adsorption of bovine serum albumin (BSA) and fibrinogen (FBG), respectively. And the platelet adhesion ability was reduced by 92.42% compared with the APLLA film. The degree of platelet activation (PF4 and TAT) and hemolysis rates of modified APLLA films also decreased significantly. Besides, the modified APLLA films promoted the growth of L929 cells and exhibited remarkable anti-inflammatory and antibacterial abilities. To summarize, the APLLA films modified with heparin-like multifunctional DA-g-SCMC2/CS multilayers exhibit outstanding biocompatibility, cytocompatibility, anti-inflammatory, and antibacterial characteristics, showcasing their potential as anticoagulation materials.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Jian Hu,Bo Liu,Tao Sun,Jiatao Zhang,Xueyan Yun,Tungalag Dong
The irreconcilable paradox between barrier performance and ductility is a “stumbling block” restricting the development of poly(L-lactic acid) (PLLA) films in the packaging industry. In this work, we reported the fabrication of an ultra-thin PLLA-based film with barrier properties and ductility by adjusting the polarity and conformational behavior of the polymer chains. Firstly, a novel unsaturated poly(L-lactic acid- co -butyrate itaconate) P(LA-BI) copolymer containing C C double bonds was synthesized using melt polycondensation. The results reveal that the addition of 60 % of P(LA-BI) enables PLLA film to achieve an elongation at a break of 83.6 % due to P(LA-BI) containing partially branched structures, which resulted in the polymer chains being arranged more in a high-energy gg conformer. Meanwhile, because of the large number of C O polar groups in P(LA-BI), PLLA/P(LA-BI) 60 film show CO 2 and O 2 permeability coefficients (CDP and OP) of 1.8 and 0.45 × 10 −8 g·m·m −2 ·h −1 ·Pa −1 respectively, which means that it has excellent gas barrier properties. Moreover, PLLA/P(LA-BI) 60 film shows a 33.3 % increase in CO 2 /O 2 ratio and an excellent ultraviolet (UV) barrier performance compared to neat PLLA. Preservation results suggested that the CO 2 and O 2 levels within the package could be regulated by varying the amount of P(LA-BI) added. Among them, PLLA/P(LA-BI) 40 film generated a more desirable CO 2 and O 2 atmosphere for cherry tomatoes preservation, which was reflected by the delaying of senescence, discoloration, and decay, inhibition of oxidative cell damage through reduced malondialdehyde production, and maintenance of nutritional and flavor substances in cherry tomatoes. This PLLA-based film offers the advantages of operational simplicity, environmental friendliness, and inexpensive cost, making it great promising for food preservation and other applications requiring barrier properties and ductility.
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