JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-23
·
IF 10.6
Deng Zihan,Yang Chuan,Xiang Tingwen,Dou Ce,Sun Dong,Dai Qijie,Ling Zhiguo,Xu Jianzhong,Luo Fei,Chen Yueqi
Osteoarthritis (OA) is a common degenerative joint disease that can cause severe pain, motor dysfunction, and even disability. A growing body of research indicates that gut microbiota and their associated metabolites are key players in maintaining bone health and in the progression of OA. Short-chain fatty acids (SCFAs) are a series of active metabolites that widely participate in bone homeostasis. Gold nanoparticles (GNPs) with outstanding anti-bacterial and anti-inflammatory properties, have been demonstrated to ameliorate excessive bone loss during the progression of osteoporosis (OP) and rheumatoid arthritis (RA). However, the protective effects of GNPs on OA progression are not clear. Here, we observed that GNPs significantly alleviated anterior cruciate ligament transection (ACLT)-induced OA in a gut microbiota-dependent manner. 16S rDNA gene sequencing showed that GNPs changed gut microbial diversity and structure, which manifested as an increase in the abundance of Akkermansia and Lactobacillus . Additionally, GNPs increased levels of SCFAs (such as butyric acid), which could have improved bone destruction by reducing the inflammatory response. Notably, GNPs modulated the dynamic balance of M1/M2 macrophages, and increased the serum levels of anti-inflammatory cytokines such as IL-10. To sum up, our study indicated that GNPs exhibited anti-osteoarthritis effects via modulating the interaction of “microbiota-gut-joint” axis, which might provide promising therapeutic strategies for OA.
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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2018
·Vol. 56
·期 2
·754-762
·
IF 1.797
Siddeeg Azhari,Zeng Xin-An,Ammar Al-Farga,Han Zhong
The target of this research was to determine sugar profile, volatile compounds, minerals content and antioxidant properties of the Sukkari date flesh as methanolic and ethanolic extracts. Sukkari date showed sugar 78.32% (dry weight), while fibre, crude protein, ash and crude fat were 3.15, 3.01, 2.30 and 0.56, respectively. Glucose (51.80%), fructose (47.50%), while small amount sucrose, fucose and galacturonic acid were also detected. Potassium, calcium and magnesium were observed to be the predominant. Twenty-two components were identified; 5-Hydroxymethylfurfural was present in the highest amount (27.25%), followed by 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl (9.45%). Total phenolic and flavonoid content of methanolic extract were 62.50 mg GAE/100 g and 3.20 mg CE/100 g, respectively, against 60.25 mg GAE/100 g and 2.90 mg CE/100 g respectively, for ethanolic extracts. Three assays including DPPH, reducing power and ABTS radical scavenging activities showed a good antioxidant activity of date palm extract. Sukkari date was observed to have good nutritional and antioxidant characteristics and can be utilize as potential nutrition.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-14
·
IF 14.7
Wei Xinlei,Yang Xue,Hu Congcong,Li Qiangzi,Liu Qianqian,Wu Yue,Xie Leipeng,Ning Xiao,Li Fei,Cai Tao,Zhu Zhiguang,Zhang Yi-Heng P. Job,Zhang Yanfei,Chen Xuejun,You Chun
In vitro biotransformation (ivBT) facilitated by in vitro synthetic enzymatic biosystems (ivSEBs) has emerged as a highly promising biosynthetic platform. Several ivSEBs have been constructed to produce poly-3-hydroxybutyrate (PHB) via acetyl-coenzyme A (acetyl-CoA). However, some systems are hindered by their reliance on costly ATP, limiting their practicality. This study presents the design of an ATP-free ivSEB for one-pot PHB biosynthesis via acetyl-CoA utilizing starch-derived maltodextrin as the sole substrate. Stoichiometric analysis indicates this ivSEB can self-maintain NADP + /NADPH balance and achieve a theoretical molar yield of 133.3%. Leveraging simple one-pot reactions, our ivSEBs achieved a near-theoretical molar yield of 125.5%, the highest PHB titer (208.3 mM, approximately 17.9 g/L) and the fastest PHB production rate (9.4 mM/h, approximately 0.8 g/L/h) among all the reported ivSEBs to date, and demonstrated easy scalability. This study unveils the promising potential of ivBT for the industrial-scale production of PHB and other acetyl-CoA-derived chemicals from starch.
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WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2024
·Vol. 40
·期 6
·1-13
·
IF 4
Zhao Siji,Yin Ruya,Zhang Mengwei,Zhai Ziqi,Shen Zhen,Mou Yan,Xu Dan,Zhou Ligang,Lai Daowan
The endophytic fungus Berkleasmium sp. Dzf12 that was isolated from Dioscorea zingiberensis , is a proficient producer of palmarumycins, which are intriguing polyketides of the spirobisnaphthalene class. These compounds displayed a wide range of bioactivities, including antibacterial, antifungal, and cytotoxic activities. However, conventional genetic manipulation of Berkleasmium sp. Dzf12 is difficult and inefficient, partially due to the slow-growing, non-sporulating, and highly pigmented behavior of this fungus. Herein, we developed a CRISPR/Cas9 system suitable for gene editing in Berkleasmium sp. Dzf12. The protoplast preparation was optimized, and the expression of Cas9 in Berkleasmium sp. Dzf12 was validated. To assess the gene disruption efficiency, a putative 1, 3, 6, 8-tetrahydroxynaphthalene synthase encoding gene, bdpks , involved in 1,8-dihydroxynaphthalene (DHN)-melanin biosynthesis, was selected as the target for gene disruption. Various endogenous sgRNA promoters were tested, and different strategies to express sgRNA were compared, resulting in the construction of an optimal system using the U6 snRNA-1 promoter as the sgRNA promoter. Successful disruption of bdpks led to a complete abolishment of the production of spirobisnaphthalenes and melanin. This work establishes a useful gene targeting disruption system for exploration of gene functions in Berkleasmium sp. Dzf12, and also provides an example for developing an efficient CRISPR/Cas9 system to the fungi that are difficult to manipulate using conventional genetic tools.
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Frontiers of Materials Science
·
2015
·Vol. 10
·期 1
·73-79
·
IF 1
Gao Rui,Ge Wen-jun,Miao Shu,Zhang Tao,Wang Xian-ping,Fang Qian-feng
The grain morphology, nano-oxide particles and mechanical properties of oxide dispersion strengthened (ODS)-316L austenitic steel synthesized by electron beam selective melting (EBSM) technique with different post-working processes, were explored in this study. The ODS-316L austenitic steel with superfine nano-sized oxide particles of 30–40 nm exhibits good tensile strength (412 MPa) and large total elongation (about 51%) due to the pinning effect of uniform distributed oxide particles on dislocations. After hot rolling, the specimen exhibits a higher tensile strength of 482 MPa, but the elongation decreases to 31.8% owing to the introduction of high-density dislocations. The subsequent heat treatment eliminates the grain defects induced by hot rolling and increases the randomly orientated grains, which further improves the strength and ductility of EBSM ODS-316L steel.
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CHEMISTRY OF NATURAL COMPOUNDS
·
2023
·Vol. 59
·期 6
·1067-1074
·
IF 0.8
Chen Yi-Fang,Luo Ji-Feng,Zhang Yan-Ni,Wang Yue-Hu
Four flavonoid C -glycosides, including two new diglycosides, dendrodiglycosides A ( 1 ) and B ( 2 ), along with known isoviolanthin ( 3 ) and apigenin 6- C - β -D-glucopyranosyl-(1→2)-α-L-arabinopyranoside ( 4 ), were isolated from the leaves of Dendrobium officinale (Orchidaceae). Their structures were elucidated by NMR and MS. Apigenin 6- C - β -D-glucopyranosyl-(1→2)-α-L-arabinopyranoside exhibited an inhibitory effect against tyrosinase with an IC 50 value of 146.5 μg/mL.
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RARE METALS
·
2023
·Vol. 43
·期 1
·356-365
·
IF 8.8
Liao Bo-Kai,Quan Rui-Xuan,Feng Ping-Xian,Wang Huan,Wang Wei,Niu Li
Abstract Lignin is a typical biological macromolecule with a three-dimensional network structure and abundant functional groups. It has excellent ionic complexation ability and amphiphilic molecular structure characteristics. In this study, the carbon steel anticorrosion performance of sodium lignosulfonate (SLS) in an acid solution was evaluated using the weight loss method, electrochemical measurements, scanning vibration electrode technique (SVET), and surface characterization methods. SLS exhibited excellent corrosion inhibition efficiency for Q235 carbon steel in 1 mol·L -1 HCl, reaching a maximum value of 98%. A low SLS concentration of 20 mg·L -1 resulted in the maximum corrosion inhibition efficiency, which remained nearly constant at higher SLS concentrations. The SVET test demonstrated that the formation of an SLS adsorption film can impede corrosion. This study confirms the significance of the application of green biomass resources in the field of metal corrosion protection and green functional materials. Graphical abstract
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Frontiers of Chemical Science and Engineering
·
2020
·Vol. 15
·期 2
·310-318
·
IF 3.552
Guan Miao,Wang Mengfan,Qi Wei,Su Rongxin,He Zhimin
Recently, many efforts have been dedicated to creating enzyme-mimicking catalysts to replace natural enzymes in practical fields. Inspired by the pathological biomineralization behaviour of l -cystine, in this study, we constructed a laccase-like catalyst through the co-assembly of l -cystine with Cu ions. Structural analysis revealed that the formed catalytic Cu-cystine nanoleaves (Cu-Cys NLs) possess a Cu(I)-Cu(II) electron transfer system similar to that in natural laccase. Reaction kinetic studies demonstrated that the catalyst follows the typical Michaelis-Menten model. Compared with natural laccase, the Cu-Cys NLs exhibit superior stability during long-term incubation under extreme pH, high-temperature or high-salt conditions. Remarkably, the Cu-Cys NLs could be easily recovered and still maintained 76% of their activity after 8 cycles. Finally, this laccase mimic was employed to develop a colorimetric method for epinephrine detection, which achieved a wider linear range (9–455 µmol·L −1 ) and lower limit of detection (2.7 µmol·L −1 ). The Cu-Cys NLs also displayed excellent specificity and sensitivity towards epinephrine in a test based on urine samples.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 33
·期 3
·1183-1191
·
IF 2.478
Dong Yanchen,Peng Haiyi,Xue Yulei,Xing Jianping,Gu Zhongyuan,Yao Xiaogang,Lin Huixing
Polyphenylene oxide (PPO)-based microwave composite substrates filled with different volume fractions (10, 20, 30, 40, 50 vol%) of MgTiO 3 -Ca 0.7 La 0.2 TiO 3 (MTCLT) ceramics were prepared by injection-molding process. The microstructure, thermal, mechanical, and dielectric properties of the PPO/MTCLT substrates were fully studied. The results showed that PPO/MTCLT substrates with dense microstructure and uniform filler distribution were obtained when the filler fraction didn’t exceed 30vol%. Further increase of the filler fraction brought about agglomeration of ceramics and weakened interfacial bonding, producing more pores in the substrates. With increasing volume fraction of ceramics, the coefficient of thermal expansion (CTE) of the substrates decreased significantly, while the bending strength increased first and then decreased, reaching a maximum at 30 vol%. Moreover, the dielectric constant of the substrates increased remarkably, while the dissipation factor decreased first and then increased. Increasing the testing frequency had little effect on the dielectric constant but increased the dissipation factor gradually. PPO/MTCLT composite substrate with filler fraction near 30 vol% exhibited the best performance and was used to fabricate a microstrip antenna. The antenna showed good performance with antenna gain over 3.54 dB and low axial ratio below 1.24 dB. The PPO/MTCLT microwave composite substrates prepared by injection-molding process exhibited good comprehensive properties, being important candidates for microwave and millimeter-wave applications.
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Nano Research
·
2020
·Vol. 13
·期 12
·3261-3267
·
IF 8.183
Han Shenghui,Lian Gang,Zeng Xiaoliang,Cao Zhaozhen,Wang Qilong,Cui Deliang,Wong Ching-Ping
Developing metal-free and long lifetime room-temperature phosphorescence (RTP) materials has received tremendous interest due to their numerous potential applications, of which stable triplet-excited state is the core challenge. Here, boron carbon oxynitride (BCNO) dots, emitting stable blue fluorescence and green RTP, are reported for the first time. The obtained BCNO dots exhibit an unexpected ultralong RTP lifetime of 1.57 s, lasting over 8 s to naked eyes. The effective doping of carbon and oxygen elements in boron nitride (BN) actually provides a small energy gap between singlet and triplet states, facilitating the intersystem crossing (ISC) and populating of triplet excitons. The formation of compact cores via crystallization and effective inter-/intra-dot hydrogen bonds further stabilizes the excited triplet states and reduces quenching of RTP by oxygen at room temperature. Based on the water-soluble feature of BCNO dots, a novel advanced security ink is developed toward anti-counterfeiting tag and confidential information encryption. This study extends BCNO dots to rarely exploited phosphorescence fields and also provides a facile strategy to prepare ultralong lifetime metal-free RTP materials.
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Scientific Reports
·
2020
·Vol. 10
·期 1
·1-8
·
IF 3.998
Chang Chao,Wang Zongyu,Fu Benwei,Ji Yulong
Solar energy as an abundant renewable resource has been investigated for many years. Solar thermoelectric conversion technology, which converts solar energy into thermal energy and then into electricity, has been developed and implemented in many important fields. The operation of solar–thermal–electric conversion systems, however, is strongly affected by the intermittency of solar radiation, which requires installation of thermal storage subsystems. In this work, we demonstrated a new solar–thermal–electric conversion system that consists of a thermoelectric converter and a rapidly charging thermal storage subsystem. A magnetic-responsive solar–thermal mesh was used as the movable charging source to convert incident concentrated sunlight into high-temperature heat, which can induce solid-to-liquid phase transition of molten salts. Driven by the external magnetic field, the solar–thermal mesh can move together with the receding solid–liquid interface thus rapidly storing the harvested solar–thermal energy within the molten salts. By connecting with a thermoelectric generator, the harvested solar–thermal energy can be further converted into electricity with a solar–thermal–electric energy conversion efficiency up to 2.56%, and the converted electrical energy can simultaneously light up more than 40 orange-colored LEDs. In addition to stable operation under sunlight, the charged thermal storage subsystem can release the stored heat and thus enables the solar–thermal–electric system to continuously generate electricity after removal of solar illumination.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2018
·Vol. 29
·期 21
·18036-18044
·
IF 2.324
Hu Querui,Wang Yiping,Wu Lei,Yin Jiang,Chen Lang,Yuan Guoliang,Yang Ying
Piezoelectric ceramics x LiNbO 3 – y BiScO 3 –(1− x − y )PbTiO 3 (LN–BS–PT, 0.00 ≤ x ≤ 0.10, 0.30 ≤ y ≤ 0.36) were synthesized and their phase diagram and morphotropic phase boundary between rhombohedral and tetragonal phases have been confirmed. The optimal properties were found at the composition of 0.03LN–0.36BS–0.61PT with piezoelectric coefficient d 33 * value of 702 pm/V, d 33 of 551 pC/N, planar electromechanical coupling factor k p of 0.51, remnant polarization P r of 46.5 µC/cm 2 , Curie temperature T c of 337 °C, and a large strain of 0.351% at an electric field of 50 kV/cm and frequency of 2 Hz with a low strain hysteresis of 5.9%. The Curie temperature of the ternary system presents a linear relationship with LiNbO 3 and BiScO 3 contents. The optimization of these electric properties was probably ascribed to the enhancement in domain walls and the improving mobility of domain switching due to LiNbO 3 doping.
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