COLLOIDS AND SURFACES B-BIOINTERFACES
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2016
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IF 3.902
Xianqiang Li,Shuyu Xie,Yuanhu Pan,Wei Qu,Yanfei Tao,Dongmei Chen,Lingli Huang,Zhenli Liu,Yulian Wang,Zonghui Yuan
In order to effectively control the bacterial pneumonia in pigs, doxycycline hydrochloride (DoxHcl) and florfenicol (FF) microparticle suspension together with inclusion complexes was prepared by using hydroxypropyl-β-cyclodextrin (HP-β-CD) as host molecules, polyvinylpyrroliddone (PVP) as polymer carriers and hydroxypropyl methyl cellulose (HPMC) as suspending agents. In vitro antibacterial activity, properties, stability and pharmacokinetics of the suspension were studied. The results demonstrated that DoxHcl and FF had a synergistic or additive antibacterial activity against Streptococcus suis , Actinobacillus pleuropneumoniae and Haemophilus parasuis . The size, polydispersity index and zeta potential of microparticles were 1.46 ± 0.06 μm, 0.30 ± 0.02 and 1.53 ± 0.04 mV, respectively. The encapsulation efficiency (EE) of DoxHcl and FF was 45.28% ± 3.30% and 89.69% ± 2.71%, respectively. The re-dispersed time and sedimentation rate of the suspension were 1 min and 1. The suspension went through the 9-gage needle smoothly with withdrawal volume of 9.12 ± 0.87 mL/min. The suspension showed good stability when stored away from light, no irritation at the injection site and sustained release in PBS buffer. After intramuscular administration to pig, DoxHcl and FF could maintain over 0.15 μg/mL for 72 h. Compared to the control injection, the suspension increased the elimination half-life (T ½ke ) as well as mean residence time (MRT) of DoxHcl from 5.73 to 9.77 h and from 12.02 to 18.81 h, and those of FF from 12.02 to 26.19 h and from12.02 to 28.16 h, respectively. The suspension increased the bioavailability of DoxHcl and FF by 1.74 and 1.13-fold, respectively. These results suggest that the compound suspension is a promising formulation for pig pneumonia therapy.
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Materials
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2017
·Vol. 10
·期 8
·868
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IF 2.654
Wenbo Dong,Kaiyin Wang,Yu Chen,Weiping Li,Yanchun Ye,Shaohua Jin
An electrochemical detection biosensor was prepared with the chitosan-immobilized-enzyme (CTS-CAT) and β-cyclodextrin-included-ferrocene (β-CD-FE) complex for the determination of H2O2. Ferrocene (FE) was included in β-cyclodextrin (β-CD) to increase its stability. The structure of the β-CD-FE was characterized. The inclusion amount, inclusion rate, and electrochemical properties of inclusion complexes were determined to optimize the reaction conditions for the inclusion. CTS-CAT was prepared by a step-by-step immobilization method, which overcame the disadvantages of the conventional preparation methods. The immobilization conditions were optimized to obtain the desired enzyme activity. CTS-CAT/β-CD-FE composite electrodes were prepared by compositing the CTS-CAT with the β-CD-FE complex on a glassy carbon electrode and used for the electrochemical detection of H2O2. It was found that the CTS-CAT could produce a strong reduction peak current in response to H2O2and the β-CD-FE could amplify the current signal. The peak current exhibited a linear relationship with the H2O2concentration in the range of 1.0 × 10−7–6.0 × 10−3mol/L. Our work provided a novel method for the construction of electrochemical biosensors with a fast response, good stability, high sensitivity, and a wide linear response range based on the composite of chitosan and cyclodextrin.Keywords:chitosan;catalase;cyclodextrin;ferrocene;composite electrode;electrochemical sensor
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JOURNAL OF MOLECULAR LIQUIDS
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2017
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IF 3.648
Yongqin Wei,Jin Zhang,Amjad Hussain Memon,Hao Liang
Phloretin , a dihydrochalcone flavonoid , has been associated with a wide range of biological properties such as antioxidation, antitumor , antidiabetes, skin whiting, etc. However, the instability and aqueous insolubility in its native form decreases its bioavailability and make its usage limited in clinical application. In our study, an inclusion complex of phloretin with hydroxypropyl-β-cyclodextrin (HP-β-CD) that featured improved aqueous solubility and stability was prepared using a simple co-evaporation method. The inclusion complex prepared under a condition of 1:1, 50 °C, 2 h and 500 rpm showed a great enhancement of phloretin aqueous solubility of 54.08 ± 0.05 mg/mL, which was 5808 times higher than that of free phloretin in pure water at 25 °C. The results of phase solubility study and the characterization data from SEM, PXRD , FT-IR and NMR suggested that aromatic ring of one phloretin molecule was merged into the cavity of one HP-β-CD from the narrow side. The in vitro test showed that the inclusion complex still remained an approximately DPPH radical-scavenging capacity compared to the free phloretin at the same concentration. And the stability of phloretin was greatly improved after forming inclusion complex with HP-β-CD. Thus, the phloretin/HP-β-CD inclusion complex would be a promising approach in the clinical application of phloretin in the future.
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CHEMICAL ENGINEERING JOURNAL
·
2016
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IF 5.31
Luhua Jiang,Yunguo Liu,Shaobo Liu,Xinjiang Hu,Guangming Zeng,Xi Hu,Simian Liu,Shaoheng Liu,Binyan Huang,Meifang Li
A novel adsorbent, namely, β-cyclodextrin/poly ( l -glutamic acid) supported magnetic graphene oxide (CGMG), was synthesized through a facile chemical route and its application as excellent adsorbent for 17β-estradiol (E2) removal from aqueous solution was also demonstrated. The characteristics of CGMG were investigated via using Fourier transform infrared spectrometer, X-ray photoelectron spectroscopy, Raman spectra, X-ray diffraction, vibrating sample magnetometer and Brunauer-Emmett-Teller surface area, respectively. Results indicated that CGMG was successfully prepared and the saturated magnetization could reach up to 0.09043 emu/mg. Meanwhile, investigation of adsorption behavior illustrated that the adsorption process could be better fitted by the pseudo-second-order and Langmuir models. Analysis of intraparticle diffusion model demonstrated that intraparticle diffusion was not the only rate-limiting step; however, both film diffusion and intraparticle diffusion were involved in the diffusion process. Thermodynamic study indicated that adsorption of E2 onto CGMG was spontaneous and endothermic. The E2 uptake by CGMG decreased in high pH values. However, it was insensitive to ionic strength variation (0.0–0.1 M). Moreover, the regeneration experiments illustrated that CGMG could be recovered, and it showed good recycling ability with ca. 88.7% of the initial sorption capacity after being used for sixth cycles.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
·
IF 3.671
Tao Feng,Ke Wang,Fangfang Liu,Ran Ye,Xiao Zhu,Haining Zhuang,Zhimin Xu
Naringin is a bioflavonoid that is rich in citrus plants and possesses enormous health benefits. However, the use of naringin as a nutraceutical is significantly limited by its low bioavailability. In this study, a novel water-soluble ternary nanoparticle material consisting of amylose , α-linoleic acid and β-lactoglobulin was developed to encapsulate naringin to improve its bioavailability. The physicochemical characteristics of the ternary nanoparticle-naringin inclusion complex were analysed by ultraviolet-visible spectroscopy (UV), Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), high-resolution transmission electron microscopy (TEM), X-ray diffractometry (XRD) and particle size distribution. The results confirmed the formation of the ternary nanoparticle-naringin inclusion complex. The encapsulation efficiency (EE) and loading content (LC) of the ternary nanoparticle-naringin inclusion complex were 78.73 ± 4.17% and 14.51 ± 3.43%, respectively. In addition, the results of the ternary nanoparticle-naringin inclusion complex in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) demonstrated that naringin can be gradually released from the complex. In conclusion, ternary nanoparticles are considered promising carriers to effectively improve the bioavailability of naringin.
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NEW JOURNAL OF CHEMISTRY
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2017
·Vol. 41
·期 5
·2169-2177
·
IF 3.269
Min Zhang,Hua Zhang,Xingchen Zhai,Xin Yang,Haitian Zhao,Jing Wang,Aijun Dong,Zhenyu Wang
A sensitive, simple and cost-effective method for large-scale monitoring of nitenpyram (NIT) is important in food quality control. This study presents a new approach for the rapid detection of NIT using a glassy carbon electrode modified by β-cyclodextrin–reduced graphene oxide nanosheets, which exhibits a considerable improvement of the reduction peak current of NIT. After incubation at 0 °C for 8 min, the resulting electrochemical platform presented high sensitivity with a wide linear range from 5 × 10−7 M to 2.2 × 10−5 M as well as a low detection limit of 1.1 × 10−7 M by linear sweep voltammetry. In addition, water-extraction followed by liquid–liquid extraction pretreatment has been developed to improve the extraction rate and efficiently eliminate the interferent. This method has been applied for the determination of spiked NIT in three kinds of rice samples with the detection limit of 0.3 mg kg−1. The recovery was 74.56–107.15% and the results were validated through HPLC.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
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IF 3.3
Hongbo Mi,Yingci Yang,Shumin Yi,Jianrong Li,Jingxin Chen,Xuepeng Li
BACKGROUND The effect of oleogels prepared with peanut oil and different concentrations of γ -oryzanol and β -sitosterol mixture ( γ / β ; 20, 40, 60, 80 and 100 g kg −1 ) on the physicochemical and gel properties of myofibrillar protein (MP) was investigated. RESULTS The solubility and average particle size of MP first decreased and then increased with increasing γ / β concentration. Peanut oil or oleogels could induce the exposure of hydrophobic amino acids and the unfolding of MP, thus significantly increasing the surface hydrophobicity, sulfhydryl content and absolute value of zeta potential, which reached maximum values when the γ / β concentration was 60 g kg −1 ( P < 0.05). The addition of peanut oil decreased the gel strength and water holding capacity of MP gel. However, oleogels prepared with 60 g kg −1 γ / β could significantly increase the hydrophobic interactions and disulfide bond content of MP gel ( P < 0.05), which promoted the crosslinking and aggregation of MP, enhancing the gel properties. Peanut oil had no significant influence on the secondary structure of MP, while oleogels promoted the transition of MP conformation from α -helix to β -sheet structure. The results of light microscopy and confocal laser scanning microscopy indicated that oleogels prepared with 60 g kg −1 γ / β filled in the pores of MP gel network to form denser and more uniform structure. CONCLUSION Oleogels prepared with 60 g kg −1 γ / β could effectively improve the quality of MP gel and have promising application prospects in surimi products. © 2024 Society of Chemical Industry.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2024
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IF 3.8
Wei Yuxin,Bai Qingqing,Ning Xinlu,Bai Xiaofan,Lv Jie,Li Meng
Sensitive and accurate detection of the amyloid-β (Aβ) monomer is of fundamental significance for early diagnosis of Alzheimer’s disease (AD). Herein, inspired by the specific Cu-Aβ monomer coordination, a cutting-edge colorimetric assay based on single-atom Cu anchored N-doped carbon nanospheres (Cu-NCNSs) was developed for Aβ monomer detection and an amyloidogenesis study. By directly pyrolyzing Cu 2+ -incorporated covalent organic frameworks (COFs), the resulting Cu-NCNSs with a high loading of Cu (8.04 wt %) exhibited outstanding peroxidase-like activity. The strong binding affinity of Aβ monomer to Cu-NCNSs effectively inhibited their catalytic activity, providing the basis for the colorimetric assay. The Cu-NCNSs-based sensor showed a detection limit of 1.182 nM for Aβ monomer, surpassing traditional techniques in terms of efficiency, accuracy and simplicity. Moreover, the system was successfully utilized for Aβ monomer detection in rat cerebrospinal fluid (CSF). Notably, the distinct inhibitory effects of monomeric and aggregated Aβ species on the catalytic activity of Cu-NCNSs were allowed for monitoring of the dynamic aggregation process of Aβ. Compared to thioflavin T (ThT), the most widely used amyloid dye, the detection system exhibited greater sensitivity towards toxic Aβ oligomers, which was crucial for early AD diagnosis and treatment. Our work not only sheds light on the rational design of highly active single-atom nanozymes from COFs but also expands the potential applications of nanozymes in early disease diagnosis. Graphical
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Mohamed Eid,Jingsong Zhu,Muhammad Asif Ismail,Bin Li
Chemically cross-linked hydrogel nanoparticles (HGNPs) offer enhanced properties over their physical counterparts, particularly in drug delivery and cell encapsulation. This study applied pH-thermal dual responsive bio-adhesive HGNPs for dual complexation and enhanced the controlled release and bioavailability of cisplatin (CDDP) and Vitamin E (VE) drugs. The CDDP was loaded into the HGNPs via chemical conjugation with the carboxyl groups in the HGNPs surface by soy polysaccharides (SSPS). At the same time, the host-guest interaction complexed the VE through the β-cyclodextrin (β-CD). The HGNPs showed a uniform HGNPs size distribution of 90.77 ± 14.77 nm and 81.425 ± 13.21 nm before and after complexation, respectively. The FTIR, XRD , XPS , and zeta potential confirmed the conjugation. The cumulative release percent of CDDP reached 98 % at pH 1.2, while <45 % was released at pH 7.4. Our HGNPs enhance the incorporation of CDDP by substituting its chlorides with carboxyl groups of the SSPS; the loading of CDDP and VE was 15 ± 0.33 and 11.32 ± 0.25 wt%, respectively. Moreover, the CDDP and VE also released slower from the HGNPs at 25 °C than at 37 °C and 42 °C. The (VE/CDDP)-loaded HGNPs exhibited longer circulation time in vivo than free CDDP and free VE suspension.
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TALANTA
·
2024
·
IF 5.6
Sihui Ding,Yu Xu,Song Xue,Siyao Liu,Haoxiang Meng,Qi Zhang
β-cyclodextrin (β-CD), as an important pseudo-stationary phase (PSP) in capillary electrophoresis (CE), frequently confronts challenges stemming from its limited water solubility, particularly when high concentrations are required for resolving complex analytes. Traditionally, researchers often resort to the use of (toxic) organic solvents to enhance the solubility of β-CD, establishing non-aqueous capillary electrophoresis (NACE) for specific separations. However, such practices are hazardous to health and run counter to the principles of green analytical chemistry. In this study, we demonstrate a deep eutectic solvent (DES), Proline:Urea (PU), as a promising alternative to conventional organic solvents for β-CD-based CE separations. The DES exhibits a solubility of up to 30% for β-CD, a significant improvement compared to the 1.8% solubility in the aqueous phase. Utilizing this DES-type separation medium, we achieved simultaneous baseline separation of a complex analyte composed of eight structurally similar naphthoic acid derivatives. Furthermore, we conducted a systematic comparison of β-CD's performance in aqueous CE buffers, organic solvents, and DESs, highlighting the superiority of this novel and environmentally friendly CE separation medium.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2024
·
IF 4.2
Yonghua Zhang,Okwong Oketch Reymick,Bin Duan,Shenghua Ding,Rongrong Wang,Nengguo Tao
The essential oil and β -cyclodextrin inclusion complex was able to inhibit the growth of Penicillium digitatum , a damaging pathogen that causes green mold in citrus fruit. In this study, cinnamaldehyde- β -cyclodextrin inclusion complex ( β -CDCA) for controlling citrus green mold was synthesized by the co-precipitation method. Characterization of β -CDCA revealed that the aromatic ring skeleton of cinnamaldehyde (CA) was successfully embedded into the cavity of β -CD to form the inclusion complex. β -CDCA inhibited P. digitatum at a minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of 4.0 g/L. FT-IR spectroscopy analysis, calcofluor white staining, extracellular alkaline phosphatase (AKP) activity and propidium iodide (PI) staining of hyphae morphology showed that β -CDCA may damage the cell ultrastructure and membrane permeability of P. digitatum . The study further demonstrated that hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA), and reactive oxygen species (ROS) markedly accumulated in 1/2 MIC β -CDCA treated hyphae. This implied that β -CDCA inhibited growth of P. digitatum by the triggering oxidative stress, which may have caused cell death by altering cell membrane permeability. In addition, in vivo results showed that β -CDCA alone or combined with L -phenylalanine (L-PHe) displayed a comparable level to that of prochloraz. Therefore, β -CDCA combined with L-PHe can thus be used as an eco-friendly preservative for the control green mold in postharvest citrus fruit.
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EXPERIMENTAL CELL RESEARCH
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2024
·
IF 3.3
Zhen Xu,Yilin Guo,Lu Wang,Jinquan Cui
HECW1 belongs to ubiquitin ligase (E3) HECT family, and is found to be involved in tumorigenesis and tumor progression. However, the function of HECW1 in cervical cancer (CC) remains unknown. Clinical analysis showed that HECW1 is significantly decreased in CC tumor tissues. Ectopic expression of HECW1 suppressed cell growth, promoting cell cycle arrest and apoptosis in CC cells, while downregulation of HECW1 reversed these trends, impeded proliferation and accelerated cell cycle progression of CC cells. Overexpressing of HECW1 reduced mitochondrial membrane potential and the protein expression of voltage-dependent anion channel 1 (VDAC1). In addition, upregulation of HECW1 inhibited nuclear β-catenin accumulation, downregulated β-catenin/TCF/LEF-mediated transcriptional activity and the expression of downstream gene c-Myc, whereas inhibition of HECW1 received opposite results. Further results confirmed HECW1 affects the protein expression of dishevelled-1 (DVL1), a potent activator of Wnt/β‐catenin, and inhibition of HECW1 inhibited the ubiquitination of DVL1, upregulating its expression. Inhibition of DVL1 restrained the promotion effect of HECW1 suppression on cell proliferation. In vivo experiments also verified that HECW1 suppression promoted the tumor formation of CC cells. Summary, we demonstrated that HECW1 inhibits CC cell proliferation and tumor formation by downregulating DVL1 induced Wnt/β‐catenin signaling pathway activation.
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危险品化学品经营许可证（带存储）