LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Chao Zhang,Xiaoai Chen,Xiaoxue Guo,Ruijia Guo,Lin Zhu,Xinrong Qiu,Xiaohan Yu,Jun Chai,Chunhe Gu,Zhen Feng
Noni ( Morinda citrifolia L.) fruit juice (NFJ) has an unpleasant flavor but is highly bioactive, so it is necessary to improve the quality of NFJ by exploring a novel strategy. The effects of eight lactic acid bacteria (LAB) on the antioxidant, phenolic, iridoid, and flavor properties of NFJ were investigated in the present study. The results showed that antioxidant activity was improved after NFJ fermentation by LAB and that L. rhamnosus had the highest free radical scavenging activity. The highest total phenolic content and total flavonoid content were in L. brevis- fermented NFJ (78.85 mg gallic acid equivalent/100 mL and 37.12 mg rutin equivalent/100 mL, respectively). Bioconversion of iridoid revealed that deacetylasperulosidic acid can be deacetylated from asperulosidic acid. Furthermore, LAB fermentation presented a significant decrease in unpleasant butanoic acid, with the lowest content found in L. fermentum- fermented NFJ. This research provides progressive information on the application of LAB culture in NFJ fermentation.
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Processes
·
2023
·Vol. 11
·期 9
·2547
·
IF 3.5
Rui An,Wenzhu Kuang,Zijian Li,Tiancheng Mu,Hongxia Luo
In this work, carbon nano-onions (CNOs) with particle sizes of 5–10 nm were prepared by the multi-potential step method. High-resolution transmission electron microscopy, infrared spectroscopy and Raman spectroscopy characterize the effective synthesis of CNOs. CNOs/GCEs were prepared by depositing the prepared CNOs onto glassy carbon electrodes (GCEs) by a drop-coating method. Examination of the electrocatalytic activity of the CNOs/GCE sensor by simultaneously detecting dopamine (DA), uric acid (UA), L-tryptophan (Trp) and theophylline (TP) using a differential pulse voltammetry technique. The results showed that the linear ranges of DA, UA, Trp and TP were DA 0.01–38.16 μM, UA 0.06–68.16 μM, Trp 1.00–108.25 μM, and TP 8.16–108.25 μM, and the detection limits (S/N = 3) were 0.0039 μM, 0.0087 μM, 0.18 μM and 0.35 μM, respectively. The CNOS/GCE sensor had good stability and could be used for the detection of actual samples.Keywords:carbon nano-onions;electrochemical sensing;glassy carbon electrode;theophylline;uric acid;L-tryptophan;dopamine
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 27
·70109-70120
·
IF 5.8
Deng Dengxian,Wang Junxia,Xu Sijie,Sun Yueying,Shi Guangyu,Wang Huili,Wang Xuedong
This study investigated the physiological and biochemical impacts of organophosphate flame retardants (OPFRs) on wheat ( Triticum aestivum L.) germination and growth performance in the presence and absence of copper. The study evaluated seed germination, growth, OPFRs concentrations, chlorophyll fluorescence index (F v /F m and F v /F 0 ), and antioxidant enzyme activity. It also calculated the root accumulation of OPFRs and their root-stem translocation. At the germination stage, at a concentration of 20 μg·L −1 OPFR exposure, wheat germination vigor, root, and shoot lengths were significantly decreased compared to the control. However, the addition of a high concentration of copper (60 mg·L −1 ) decreased by 80%, 82%, and 87% in the seed germination vitality index and root and shoot elongation, respectively, compared to 20 μg·L −1 of OPFR treatment. At the seedling stage, a concentration of 50 μg·L −1 of OPFRs significantly decreased by 42% and 5.4% in wheat growth weight and the photochemical efficiency of photosystem II (Fv/Fm) compared to the control. However, the addition of a low concentration of copper (15 mg·L −1 ) slightly enhanced the growth weight compared to the other two co-exposure treatments, but the results were not significant ( p > 0.05). After 7 days of exposure, the activity of superoxide dismutase (SOD) and malondialdehyde (MDA) (indicates lipid peroxidation) content in wheat roots significantly increased compared to the control and was higher than in leaves. MDA contents in wheat roots and shoots were decreased by 18% and 6.5% when OPFRs were combined with low Cu treatment compared with single OPFRs treatment, but SOD activity was slightly improved. These results suggest that the co-exposure of copper and OPFRs enhances reactive oxygen species (ROS) production and oxidative stress tolerance. Seven OPFRs were detected in wheat roots and stems, with root concentration factors (RCFs) and translocation factors (TFs) ranging from 67 to 337 and 0.05 to 0.33, respectively, for the seven OPFRs in a single OPFR treatment. The addition of copper significantly increased OPFR accumulation in the root and aerial parts. In general, the addition of a low concentration of copper promoted wheat seedling elongation and biomass and did not significantly inhibit the germination process. OPFRs could mitigate the toxicity of low-concentration copper on wheat but had a weak detoxification effect on high-concentration copper. These results indicated that the combined toxicity of OPFRs and Cu had antagonistic effects on the early development and growth of wheat.
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高影响因子
Chinese Medicine
·
2023
·Vol. 18
·期 1
·1-20
·
IF 4.9
Xu Yang-ding,Guo Yu-jie,Mao He-rong,Xiong Zhi-xiang,Luo Meng-yu,Luo Rui-qi,Lu Shan,Huang Lu,Hong Yi
Background Dermatophyte caused by Trichophyton mentagrophytes is a global disease with a growing prevalence that is difficult to cure. Perilla frutescens (L.) Britt. is an edible and medicinal plant. Ancient books of Traditional Chinese Medicine and modern pharmacological studies have shown that it has potential anti-fungi activity. This is the first study to explore the inhibitory effects of compounds from P. frutescens on Trichophyton mentagrophytes and its mechanism of action coupled with the antifungal activity in vitro from network pharmacology, transcriptomics and proteomics.Methods Five most potential inhibitory compounds against fungi in P. frutescens was screened with network pharmacology. The antifungal activity of the candidates was detected by a broth microdilution method. Through in vitro antifungal assays screening the compound with efficacy, transcriptomics and proteomics were performed to investigate the pharmacological mechanisms of the effective compound against Trichophyton mentagrophytes. Furthermore, the real-time polymerase chain reaction (PCR) was applied to verify the expression of genes.Results The top five potential antifungal compounds in P. frutescens screened by network pharmacology are: progesterone, luteolin, apigenin, ursolic acid and rosmarinic acid. In vitro antifungal assays showed that rosmarinic acid had a favorable inhibitory effect on fungi. The transcriptomic findings exhibited that the differentially expressed genes of fungus after rosmarinic acid intervention were mainly enriched in the carbon metabolism pathway, while the proteomic findings suggested that rosmarinic acid could inhibit the average growth of Trichophyton mentagrophytes by interfering with the expression of enolase in the glycolysis pathway. Comparison of real-time PCR and transcriptomics results showed that the trends of gene expression in glycolytic, carbon metabolism and glutathione metabolic pathways were identical. The binding modes and interactions between rosmarinic acid and enolase were preliminary explored by molecular docking analysis.Conclusion The key findings of the present study manifested that rosmarinic acid, a medicinal compound extracted from P. frutescens, had pharmacological activity in inhibiting the growth of Trichophyton mentagrophytes by affecting its enolase expression to reduce metabolism. Rosmarinic acid is expected to be an efficacious product for prevention and treatment of dermatophytes.
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Analytical Methods
·
2023
·Vol. 15
·期 26
·3251-3258
·
IF 3.1
Feifei Li,Congcong Hu,Wenen Su,Hao Liang,Fubing Xiao,Jinquan Liu,Yan Tan,Shengyuan Yang
L-Cysteine, widely used in medicine and the food industry, is of great essentiality to organisms and the food quality. Given that current detection approaches require exacting lab conditions and tedious sample treatment, there is a pressing demand for developing a method that possesses advantages of user friendliness, prominent performance, and cost-effectiveness. Herein, a self-cascade system was developed for the fluorescence detection of L-cysteine based on the ingenious performance of Ag nanoparticle/single-walled carbon nanotube nanocomposites (AgNP/SWCNTs) and DNA-templated Ag nanoclusters (DNA-AgNCs). The fluorescence of DNA-AgNCs could be quenched on account of the adsorption of DNA-AgNCs on AgNP/SWCNTs by π–π stacking. With the cooperation of Fe2+, AgNP/SWCNTs with oxidase and peroxidase-like activities could catalyze the oxidation of L-cysteine to produce cystine and hydrogen peroxide (H2O2) and then break the O–O bond of H2O2 to generate a hydroxyl radical (·OH), which could cleave the DNA strand into different sequence fragments which subsequently peeled off from the AgNP/SWCNTs, resulting in a “turn-on” fluorescence response. In this paper, AgNP/SWCNTs with multi-enzyme activities was synthesized enabling the reaction to proceed in just one step. The successful preliminary applications for the L-cysteine detection in pharmaceutical, juice beverage, and serum samples indicated that the developed method exhibited great potential in medical diagnosis, food monitoring, and the biochemical field, which also broadened the horizon for follow-up research.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 14
·2677-2692
·
IF 4.3
Meng Xin,Zong Hou,Zheng Zhong,Xing Junpeng,Liu Zhiqiang,Song Fengrui,Liu Shu
α-Glucosidase inhibitors in natural products are one of the promising drugs for the treatment of type 2 diabetes. However, due to the complexity of the matrix, it is challenging to comprehensibly clarify the specific pharmacodynamic substances. In this study, a novel high-throughput inhibitor screening strategy was established based on covalent binding of α-glucosidase on chitosan-functionalized multi-walled carbon nanotubes coupled with high-resolution mass spectrometry. The synthesized MWCNTs@CS@GA@α-Glu was characterized by TEM, SEM, FTIR, Raman, and TG. Performance studies showed that the microreactor exhibited stronger thermostability and pH tolerance than that of the free one while maintaining its inherent catalytic activity. Feasibility study applying a model mixture of known α-glucosidase ligand and non-ligands indicated the selectivity and specificity of the system. By integrating ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-QTOF-MS) with ion mobility mass spectrometry (IMS), 15 ligands were obtained and tentatively identified from Tribulus terrestris L., including 8 steroidal saponins, 4 flavonoids, and 3 alkaloids. These inhibitors were further validated by in vivo experiments and molecular docking simulation.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2023
·
IF 5.8
Yue Huang,Chenguang Yang,Jianlin lv,Yu Zhang,Kun Wang,Lili Ma,Zhilin Liu,Haiyang Yu,Mingqiang Li,Zhaohui Tang
Poly(L-glutamic acid)- g -methoxy poly(ethylene glycol)/Combretastatin A4 (CA4)/BLZ945 nanoparticles (CB-NPs) have shown great potential in synergistic cancer therapy. However, it is still unclear how the nanoparticles’ formula, such as injection dose, active agent ratio, and drug loading content, affects the side effects and in vivo efficacy of CB-NPs. In this study, a series of CB-NPs with different BLZ945/CA4 (B/C) ratios and drug loading contents were synthesized and evaluated on a hepatoma (H22) tumor-bearing mice model. The injection dose and B/C ratio were found to have a significant influence on the in vivo anticancer efficacy. The CB-NPs 20 with B/C weight ratio of 0.45/1, and total drug loading content (B + C) of 20.7 wt%, showed the highest potential for clinical application. Systematic pharmacokinetics , biodistribution , and in vivo efficacy evaluation for CB-NPs 20 have been finished, which may provide significant instruction for medicine screening and clinical application.
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高影响因子
JOURNAL OF FLUORESCENCE
·
2022
·Vol. 33
·期 3
·1111-1123
·
IF 2.525
Zhang Dianwei,Zhang Furui,Wang Shengnan,Hu Sha,Liao Yonghong,Wang Fenghuan,Liu Huilin
Amino- and sulfhydryl- functionalized biomass carbon dots (BCDs) were prepared by one-pot reverse microemulsion for specific recognition of ferric ions (Fe 3+ ) and L-cysteine (L-Cys). Green grapefruit peel was used as the carbon source while aminosilane and mercaptosilane were used as N- and S-supplier. Following the adsorption of Fe 3+ on the surfaces of BCDs-NH 2 and BCDs-SH, the fluorescence responses was quenched step by step, while adding L-Cys to the BCDs-NH 2 /Fe 3+ system restored the fluorescence. The BCDs-NH 2 and BCDs-SH system exhibited extremely low limits of detection for Fe 3+ of 3.2 and 3.0 nM, respectively, within a wide linear ranges of 0.006–200 μM and 0.004–200 μM, respectively. The BCDs-NH 2 /Fe 3+ systems were used as an optosensor for L-Cys in the concentration ranges of 0.08–30 and 30–1000 μM with a detection limit of 65 nM. Developed BCDs-NH 2 and BCDs-SH were able to respond to Fe 3+ in water samples with satisfactory recoveries of 100.1%-103.1% and 94.6%-108.5%, respectively, and the BCDs-NH 2 /Fe 3+ system was also able to respond to BCDs-NH 2 /Fe 3+ in actual lake water samples with recoveries from 87.3% to 98.8%. Meanwhile, The BCDs-NH 2 exhibited good photoluminescence and stability, and the with a fluorescence quantum yield was as high as 25%. This work demonstrates the feasibility of using such materials to remove hazardous ions from water and employing the resulting complexes for optosensing in a sustainable manner.
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CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Wenjun Liu,Ling Zhu,Yunfa Ma,Lihao Ai,Wei Wen,Changren Zhou,Binghong Luo
In order to better utilize and combine the advantages of poly( d , l -lactide) (PDLLA) matrix and natural polysaccharide chitin whiskers (CHWs), needle-like CHWs with chiral nematic liquid crystal characteristics and remarkable moduli were chosen to surface modify PDLLA film. By a vertical coating method, a stable and well-ordered CHWs coating was formed on the surface of the PDLLA film through a polydopamine (PDA) interlayer. The high stability of the well-ordered CHWs coating on the surface of the PDLLA film was confirmed by PBS soaking experiment in terms of the changes of weight and surface morphology of the film. The surface microstructure and composition of the resulting PDLLA-PDA-CHWs film were characterized by field emission scanning electron microscopy (FESEM), atomic force microscope (AFM) and X-ray photoelectron spectroscopy (XPS), and the thickness of the PDLLA, PDA and CHWs layers was determined using FESEM, too. Comparing with traditional PDLLA/CHWs film, prepared by solution blending with optimal content of 5 wt% CHWs, the hydrophilicity and mechanical properties of the PDLLA-PDA-CHWs film were significantly improved owing to the immobilization of a highly ordered CHWs coating. Furthermore, in vitro cell culture showed that the well-ordered CHWs coating had a more significant effect on promoting the adhesion, spreading, proliferation and osteogenic differentiation of MC3T3-E1 cells. All these results showed that the resulting PDLLA-PDA-CHWs film with well-ordered CHWs coating designed through simple and effective approach has promising application in bone repair material field.
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TALANTA
·
2019
·
IF 4.916
Wenfang Deng,Chang Cheng,Hui Yang,Hui Wang,Yueming Tan,Qingji Xie,Ming Ma,Shouzhuo Yao
We report here a sensitive method for immunoassay of Staphylococcus aureus ( S. aureus ) based on colorimetric and fluorescent responses of 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) to l -cysteine (Cys). S. aureus cells were separated from samples by immunomagnetic nanoparticles and labeled with Cys-encapsulated liposome ( [email protected] ) immuno-nanocapsules. After mangnetic separation, the [email protected] immuno-nanocapsules linked to bacterial cells were destructed by Tween-20, and then the released Cys molecules reacted with NBD-Cl to generate colorimetric and fluorescent signals. Due to the amplification effect of liposome nanocapsules , the high capture efficiency of immunomagnetic nanoparticles, and the high responses of NBD-Cl to Cys, the detection limits for colorimetric and fluorescent detection of S. aureus were as low as 10 and 1 CFU mL −1 , respectively. The proposed method was successfully used to detect S. aureus in milk samples. This work has provided a novel NBD-Cl based colorimetric and fluorescent platform for immunoassay.
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Nanoscale
·
2018
·Vol. 10
·期 31
·14931-14937
·
IF 7.233
Juan Li,Cheng Yang,Wen-Long Wang,Xiu-Ping Yan
In vitro diagnostic is a crucial component of healthcare systems for early diagnosis of diseases and follow-up therapy, which generally makes clinical diagnosis faster, easier, and painless for patients. Herein, we report a dual-signaling nanoplatform for ratiometric absorption and time-resolved fluorescence resonance energy transfer based on L-cysteine-mediated aggregated gold nanoparticles and long afterglow nature of persistent luminescence nanoparticles. With this nanoplatform, we have achieved high-throughput sequential detection of L-cysteine and insulin in human serum without matrix interference. The proposed nanoplatform shows excellent linearity and precision for the determination of L-cysteine in the range of 10 nM to 5.5 μM with the limit of detection (LOD) of 2.2 nM and for the detection of insulin in the range of 12 pM to 3.44 nM with LOD of 2.06 pM. The developed dual-signaling nanoplatform has been successfully applied for the sequential determination of L-cysteine and insulin in human serum.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 19
·15743-15749
·
IF 3.201
Jing Wang,Daquan Li,Xinyue Liu,Yu Qiu,Xiao Peng,Liang Huang,Huimin Wen,Jun Hu
Aqueous N-acetyl-L-cysteine (NAC)-capped CdTe quantum dots (QDs) are prepared in one step by using sodium tellurite as a tellurium source and sodium borohydride as a reductant. The fluorescence (FL) emissions of the QDs are tunable between 514 and 753 nm. The effects of NAC/Cd, Cd/Te molar ratios, and pH values on the photoluminescence quantum yield (PLQY) of the QDs have been systematically studied. They display a high PLQY of up to 52.83%, with uniform narrow-size distributions. Furthermore, the obtained green- and red-emissive CdTe QDs are applied to explore the impact of QD size on the FL response toward glutathione (GSH) by a “turn-on” strategy. The results imply that the large-sized CdTe QDs with red FL are more suitable for detecting GSH than the small-sized QDs with green FL. The possible mechanism is also investigated.
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