APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2024
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IF 3.1
An Le,Yu Yang,He Long,Xiao Xu,Li Pengcheng
There are numerous therapeutic applications for ginsenoside Rb1 (GRb1), the primary saponin derived from ginseng root. According to earlier research, ginsenoside Rb1 causes apoptosis and reduces the cell cycle. Its adverse effects, especially those on the development of the embryo, still need to be thoroughly studied. A host’s lifestyle choices, including smoking, drinking too much alcohol, using tobacco products, and having an HPV infection, can increase the risk of oral squamous cell carcinoma (OSCC), one of the most prevalent malignancies of the oral cavity. To address this challenge, this investigation focuses on the design of GRb1 for treating OSCC. In vitro cytotoxicity studies confirmed that GRb1 was more effective in PCI-9A and PCI-13 cells, with reduced toxicity in non-cancerous cells. Further verification of cellular morphology was achieved through various biochemical staining methods. The mechanism of cell death was investigated by Annexin V-FITC and PI methods. Additionally, the antimetastatic attributes of GRb1 have been evaluated using both migration scratch and Transwell migration assays, which have collectively revealed excellent antimetastatic potential. The DNA fragmentation of the PCI-9A and PCI-13 cells was assessed using a comet assay. Ginsenoside Rb1 improved ROS levels and caused mitochondrial membrane potential alterations and DNA damage, which resulted in apoptosis. OSCC administration significantly reduced the levels of SOD, GSH, GPx, and CAT, increasing the levels of PCI-9A and PCI-13 cells, while GRb1 improved this situation. Therefore, we propose that Ginsenoside Rb1 could be an alternative therapeutic strategy for OSCC therapy.
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Food and Bioprocess Technology
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2024
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IF 5.3
Jiang Jingya,Song Xiaoning,Zhao Liming,Wang Song,Hou Baochao,Li Baolei,Dudu Olayemi Eyituoyo,Yi Huaxi,Zhang Lanwei,Gong Pimin
Spray drying is a promising technique for embedding and stabilizing lactic acid bacteria. However, bacteria are unable to withstand high-temperature environment. Naturally small molecules fall short in meeting the evolving demands of production. Therefore, it is imperative to explore non-chemical pretreatment methods urgently, with the aim of enhancing their protective efficacy. In this study, natural deep eutectic solvents (NADES) were synthesized by glycerin-betaine to improve the survival rates of spray-dried Lactobacillus bulgaricus sp1.1. Results revealed that the spray drying survival rate of L. bulgaricus sp1.1 after adding NADES was 30.54%, which was 4.72 times higher than adding equal amounts of untreated glycerin and betaine, and the number of viable bacteria decreased less than 1 log after 6 months of storage. The investigation revealed that the impact of NADES was localized external to the cell rather than within. Through crosslinking with the peptidoglycan on the cell wall, NADES bolstered the cell wall structure thereby creating a protective barrier. This resulted in a reduction of damage to the membrane and ribosome induced by high temperatures during the lactic acid bacteria’s exposure. This study synthesized NADES using current protective agents as an effective method to enhance heat resistance of lactic acid bacteria for spray drying for the first time, and the lactic acid bacteria powder with high survival rate and high storage stability was successfully prepared, which provides a new approach for its industrial application. Graphical Abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2024
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IF 3.8
Lv Jie,He Chengcai,Cao Jingcheng,Shuai Qiuyan,Liu Xifang,Li Meng,Lin Yulong
The abnormal expression of acetylcholinesterase (AChE) is linked to the development of various diseases. Accurate determination of AChE activity as well as screening AChE inhibitors (AChEIs) holds paramount importance for early diagnosis and treatment of AChE-related diseases. Herein, a fluorescent and colorimetric dual-channel probe based on gold nanoclusters (AuNCs) and manganese dioxide nanosheets (MnO 2 NSs) was developed. The fluorescence of AuNCs was suppressed in the presence of MnO 2 NSs, providing a platform for fluorescence-based detection. For colorimetric, the nanocomposites exhibited oxidase-like activity, rapidly catalyzing 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a blue color. Thiocholine (TCh), produced through the enzymatic reaction of acetylthiocholine (ATCh) in the presence of AChE, can reduce the MnO 2 NSs, thus recovering the suppressed fluorescence of AuNCs and decreasing oxidase-like activity. Based on this principle, a dual-mode assay for AChE detection was achieved. Compared to the commonly used single-signal detection, multi-mode detection can offer reliable and accurate results, due to its inherent self-validation and self-regulation capabilities. Under optimum conditions, the limit of detection (LOD) for AChE activity was 0.067 mU mL –1 (fluorescent mode) and 0.042 mU mL –1 (colorimetric mode). Moreover, the probe realized AChE detection in biological samples and AChEIs screening. This work showed the great prospects for early diagnosis of AChE-related diseases. Graphical
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RARE METALS
·
2024
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IF 9.6
Wan Si-Jie,Hou Yan-Ting,Wang Wang,Luo Guo-Qiang,Wang Chuan-Bin,Tu Rong,Cao Shao-Wen
Photocatalytic reduction of CO 2 has attracted considerable interest owing to its potential to resolve the energy and environmental problems. Nevertheless, the lack of proficient photocatalysts has restricted the application of solar-driven photocatalytic CO 2 reduction. Herein, we reported an S-scheme heterojunction by combining g-C 3 N 4 with La 3+ and Rh 3+ co-doped SrTiO 3 through the electrostatic self-assembly method for the efficient photocatalytic CO 2 reduction. In comparison with g-C 3 N 4 , the as-prepared CN/LRSTO-30 wt% S-scheme heterojunction not only possesses a broadened visible-light response due to the defect states in La, Rh co-doped SrTiO 3 induced by co-doped La 3+ and Rh 3+ but also has more adsorption sites for the capture and activation of CO 2 molecules. Additionally, separation and transfer efficiency of the photoinduced charge carriers is much enhanced in the CN/LRSTO-30 wt% S-scheme heterojunction via its robust internal electric field, which is firmly demonstrated by in situ irradiation X-ray photoelectron spectroscopy technology. Consequently, the prepared S-scheme heterojunction achieves impressive photocatalytic CO 2 reduction performance with an average CO and CH 4 evolution rate of 4.1–1.8 μmol·g −1 ·h −1 , respectively, which are ~ 1.9 and ~ 22.5-fold higher than those of pure g-C 3 N 4 . This study provides innovative perspectives on the design of creative S-scheme heterojunctions for applications in photocatalytic CO 2 reduction. Graphical abstract
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 3
·1-12
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IF 5.3
Wang Hongjun,Bian Ziyang,Wang Yue,Yang Zhenshan,Niu Huijuan,Li Hefu
A dual-functional biomimetic surface-enhanced Raman scattering (SERS) substrate composed of grating/microcavity arrays (GCA) was developed for on-site monitoring of pesticides on fruit peels and in surface water. The hierarchical GCA structure was fabricated by molding a negative polydimethylsiloxane (PDMS) replica from lily petals, followed by the generation of grating wrinkles through oxygen plasma treatment. After the deposition of silver nanoparticles (Ag NPs) through magnetron sputtering, the optimal Ag NPs@GCA substrate exhibits high sensitivity, good uniformity, and reproducibility. Moreover, due to its optical transparency, mechanical stability, and hydrophobic properties, the Ag NPs@GCA substrate combined with a handhold Raman spectrometer can be used for on-site determination of pesticides in real samples. The limit of detection (LOD) for thiram on the surfaces of apples, pears, and cherry tomatoes were 2.86 × 10 −7 , 5.03 × 10 −7 , and 7.25 × 10 −7 M, respectively. For malachite green, the LODs were 9.04 × 10 −8 M in lake water and 4.83 × 10 −7 M on fish scales. The results indicate the developed dual-functional Ag NPs@GCA substrate has great potential for applications in food safety assessment and environmental pollutant monitoring. Graphical abstract
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Nature Protocols
·
2024
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IF 13.1
Feng Xin,Shen Ao,Zhang Wei,Jia Shengnan,Iliuk Anton,Wang Yuling,Zhang Wenke,Zhang Ying,Tao W. Andy,Hu Lianghai
Extracellular vesicles (EVs) are small particles with phospholipid bilayers that carry a diverse range of cargoes including nucleic acids, proteins and metabolites. EVs have important roles in various cellular processes and are increasingly recognized for their ubiquitous role in cell–cell communications and potential applications in therapeutics and diagnostics. Although many methods have been developed for the characterization and measurement of EVs, analyzing them from biofluids remains a challenge with regard to throughput and sensitivity. Recently, we introduced an approach to facilitate high-throughput analysis of EVs from trace amounts of sample. In this method, an amphiphile–dendrimer supramolecular probe (ADSP) is coated onto a nitrocellulose membrane for array-based capture and to enable an in situ immunoblotting assay. Here, we describe the protocol for our array-based method of EV profiling. We describe an enhanced version of the method that incorporates an automated printing workstation, ensuring high throughput and reproducibility. We further demonstrate the use of our array to profile specific glycosylations on the EV surface using click chemistry of an azide group introduced by metabolic labeling. In this protocol, the synthesis of ADSP and the fabrication of ADSP nitrocellulose membrane array can be completed on the same day. EVs are efficiently captured from biological or clinical samples through a 30-min incubation, followed by an immunoblotting assay within a 3-h window, thus providing a high-throughput platform for EV isolation and in situ targeted analysis of EV proteins and their modifications.
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npj Flexible Electronics
·
2024
·Vol. 8
·期 1
·1-10
·
IF 12.3
Wang Chenhao,Wang Zhengyu,Wei Wei,Zhang Zhenjie,Li Anne Ailina,Huang Guanghao,Li Xian,Ge Shuzhi Sam,Zhou Lianqun,Kong Hui
As a stress hormone existing in the human body, cortisol can reflect the psychological stress and health status in daily life, and is a potential biomarker of the body’s stress response. To effectively collect sweat and accurately identify the target, this paper reports a flexible wearable cortisol detection device with outstanding reliability and sensitivity. Molecular imprinted polymer (MIP) ensures cortisol specificity. And carbon nanotubes (CNT) on electrodes increase sensitivity, expanding the detection range to 10 −3 to 10 4 nM, with sensitivity at 189.2 nA/lg(nM). In addition, porous chitosan hydrogel (PCSH) collects sweat effectively, its adhesive properties and 80% swelling rate offer a low-cost alternative to microfluidics. Flexible printed circuit board (FPCB) and serpentine electrode (SE) ensure device durability. This non-invasive, highly sensitive device offers a novel method for mental stress monitoring and clinical diagnosis, advancing human physiological state monitoring.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-14
·
IF 15.7
He Zhe,Li Kailang,Chen Tianxiang,Feng Yunchao,Villalobos-Portillo Eduardo,Marini Carlo,Lo Tsz Woon Benedict,Yang Fuyuan,Zhang Liang,Liu Lichen
Liquid organic hydrogen carriers (LOHCs) are considered promising carriers for large-scale H 2 storage and transportation, among which the toluene-methylcyclohexane cycle has attracted great attention from industry and academia because of the low cost and its compatibility with the current infrastructure facility for the transportation of chemicals. The large-scale deployment of the H 2 storage/transportation plants based on the toluene-methylcyclohexane cycle relies on the use of high-performance catalysts, especially for the H 2 release process through the dehydrogenation of methylcyclohexane. In this work, we have developed a highly efficient catalyst for MCH dehydrogenation reaction by incorporating subnanometer PtFe clusters with precisely controlled composition and location within a rigid zeolite matrix. The resultant zeolite-encapsulated PtFe clusters exhibit the up-to-date highest reaction rate for dehydrogenation of methylcyclohexane to toluene, very high chemoselectivity to toluene (enabling the production of H 2 with purity >99.9%), remarkably high stability (>2000 h) and regenerability over consecutive reaction-regeneration cycles.
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Nano Research
·
2024
·
IF 9.5
Zhao Yuan,Wang Qingshuang,Yin Dongming,Wang Xiaoli,Li Shouliang,Wang Chunli,Liang Long,Zhao Shaolei,Wang Limin,Cheng Yong
α-AlH 3 is regarded as one of the most promising hydrogen storage materials due to its high hydrogen storage capacity (10.1 wt.%, 148 kg·m −3 ). However, in practical applications, the associated hydrogen release temperature remains relatively high. To effectively address this issue, hollow structured Fe@C nanorods derived from Fe-MOF are introduced as highly efficient catalyst to optimize the dehydrogenation properties of α-AlH 3 . Comparatively, the initial hydrogen release temperature of α-AlH 3 + 3 wt.% Fe@C is reduced to 94.2 °C, which is significantly lower than that of pure α-AlH 3 (137.8 °C). At 100 and 120 °C, it exhibits hydrogen capacities of 5.38 wt.% and 7.47 wt.%, respectively, whereas pure α-AlH 3 only delivers hydrogen capacities of 0.24 wt.% and 5.94 wt.% under the same temperatures. The density functional theory (DFT) calculations further indicate that the existence of Fe@C catalyst can make the length of Al–H bond increase, which is more conducive to the release of hydrogen. The results show that the synergistic effect of Fe and porous carbon in Fe@C nanorods can improve the hydrogen desorption kinetics of α-AlH 3 , providing a good prospect for the application of α-AlH 3 in hydrogen storage fields.
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CHINESE JOURNAL OF POLYMER SCIENCE
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2024
·
IF 4.1
Wang Meng-Yun,Zhang Yue,Wang Dan,Yao Ming,Wang Yi-Xuan,Zhou Xing-Ping,Peng Hai-Yan,Xie Xiao-Lin
Holographic optical elements (HOEs) based on polymer composites have become a research hot spot in recent years for augmented reality (AR) due to the significant improvement of optical performance, dynamic range, ease of processing and high yield rate. Nevertheless, it remains a formidable challenge to obtain a large field of view (FOV) and brightness due to the limited refractive index modulation. Herein, we report an effective method to tackle the challenge by doping an epoxy liquid crystal termed E6M, which enables a large refractive index modulation of 0.050 @ 633 nm and low haze of 5.0% at a doping concentration of 5 wt%. This achievement may be ascribed to the improved molecular ordering of liquid crystals within the holographic polymer composites. The high refractive index modulation can endow transmission-type holographic polymer composites with a high diffraction efficiency of 96.2% at a small thickness of 5 µm, which would promise the design of thin and lightweight AR devices.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2024
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IF 3.5
Wang Lingqin,Jia Mengya,Gao Dandan,Li Haixing
The probiotic fermentation of the bioactive substance gamma-aminobutyric acid (GABA) is an attractive research topic. There is still room for further improvement in reported GABA fermentation methods based on a single substrate ( l -glutamic acid or l -monosodium glutamate). Here, we devised a pH auto-buffering strategy to facilitate the fermentation of GABA by Levilactobacillus brevis CD0817. This strategy features a mixture of neutral monosodium l -glutamate plus acidic l -glutamic acid as the substrate. This mixture provides a mild initial pH; moreover, the newly dissolved l -glutamic acid automatically offsets the pH increase caused by substrate decarboxylation, maintaining the acidity essential for GABA fermentation. In this study, a flask trial was first performed to optimize the GABA fermentation parameters of Levilactobacillus brevis CD0817. The optimized parameters were further validated in a 10 L fermenter. The flask trial results revealed that the appropriate fermentation medium was composed of powdery l -glutamic acid (750 g/L), monosodium l -glutamate (34 g/L [0.2 mol/L]), glucose (5 g/L), yeast extract (35 g/L), MnSO 4 ·H 2 O (50 mg/L [0.3 mmol/L]), and Tween 80 (1.0 g/L). The appropriate fermentation temperature was 30 °C. The fermenter trial results revealed that GABA was slowly synthesized from 0–4 h, rapidly synthesized until 32 h, and finally reached 353.1 ± 8.3 g/L at 48 h, with the pH increasing from the initial value of 4.56 to the ultimate value of 6.10. The proposed pH auto-buffering strategy may be popular for other GABA fermentations.
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FIBERS AND POLYMERS
·
2024
·Vol. 25
·期 10
·3661-3671
·
IF 2.2
Molla Aniruddha,Lee Jeongui,Park Eunji,Youk Ji Ho
The catalytic activity of FeCo alloy nanoparticles (NPs) supported on nitrogen-doped mesoporous carbon spheres (FeCo/NMCS) was evaluated for hydrogen evolution and reduction of various organic dyes. The NMCS was spherical, with an average diameter of 488.1 nm, while the FeCo alloy NPs were evenly distributed across the NMCS, with an average diameter of 8.2 nm. The nitrogen content in FeCo/NMCS was found to be 3.91 wt%. The FeCo/NMCS displayed remarkable efficiency in hydrogen evolution through the hydrolysis of ammonia borane (AB), characterized by an activation energy of 23.49 kJ/mol. The FeCo/NMCS also demonstrated superior catalytic activity in reducing 4-nitrophenol (4-NP), with a reduction rate constant of 0.468 min⁻ 1 using 5 mg of FeCo/NMCS and 20 mg of AB. This rate places the FeCo/NMCS among the highest-performing catalysts for 4-NP reduction. Further, the catalyst efficiently reduced crystal violet, methylene blue, and rhodamine B under the same conditions. Azo dyes, including tartrazine, methyl orange, Red 195, and Yellow 145, underwent faster reduction than other tested dyes. The FeCo/NMCS maintained its integrity and activity even after four hydrogen generation cycles. These results highlight FeCo/NMCS’s potential as a multifunctional, magnetically separable, and reusable catalyst for efficient hydrogen production and environmental remediation applications.
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