INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2016
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IF 3.994
Xiao-Bin Fang,Jin-Ming Zhang,Xi Xie,Di Liu,Cheng-Wei He,Jian-Bo Wan,Mei-Wan Chen
Curcumin (Cur) is a highly pleiotropic anticancer agent that inhibits cell proliferation and induces apoptosis in cancer cells. A variety of nano-systems constituted by polymer–drug conjugates have been designed to overcome its shortages on water solubility, chemical instability, and poor bioavailability. However, most of them suffer from ineffective release of Cur in cancer cells in vivo . This work developed a novel flexible acid-responsive micelle formulation by covalently conjugating Cur on the hydrophilic terminals of pluronic F68 chains via cis -aconitic anhydride linkers. The synthesized F68-Cis–Cur conjugates can readily precipitate to form homogeneous micelles with average size about 100 nm in aqueous solution. In acid environments, F68-Cis–Cur conjugates would break down and subsequently release Cur rapidly, for the reason of pH-sensitive cleavage of cis -aconitic anhydride linkers. In vitro anticancer activity tests demonstrated that F68-Cis–Cur micelles induced higher cytotoxicity against both A2780 and SMMC 7721 cells than free Cur. It provided a larger decrease of mitochondrion membrane potential and induced cellular apoptosis. F68-Cis–Cur micelles remarkably increased cellular uptake of Cur than free Cur through caveolae-mediated endocytosis in an energy-dependent manner. This study demonstrates F68-Cis–Cur conjugation as a promising tool for improving intracellular drug delivery in cancer therapy.
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JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2020
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IF 2.69
Zhentao Lei,Qiuju Tang,Yanshan Ju,Yonghui Lin,Xiaowen Bai,Haipeng Luo,Zaizai Tong
Developing the hybrid nanosystems for controlled drug release is still a challenging task. In this work, pH-responsive core-shell nanocomposites have been prepared by the growth of zeolitic imidazolate framework-8 (ZIF-8) on the surface of polymeric aggregates self-assembled from poly(ε-caprolactone)-block-poly (quaternized vinylbenzyl chloride/bipyridine) (PCL-b-q(PVBC/BPy), BCP for short) in water. The core of the micelles or the inner cavity of vesicles serves as the drug storage reservoir for the doxorubicin hydrochloride (DOX) and the ZIF-8 shells act as the gatekeepers to prevent drug premature release at physiological environment. Upon pH stimulus, the core-shell nanocomposites (BCP@ZIF-8) show a retarded drug release behavior compared with DOX-loaded polymeric aggregates counterparts (without the shell of ZIF-8). Moreover, the as-prepared nanocomposites perform good biocompatibility towards MCF-7 cell. Meanwhile, the DOX-loaded BCP@ZIF-8 nanocomposites present lower cytotoxicity compared with DOX-loaded BCP and free DOX. The confocal microscopy study shows the core-shell nanocomposites could be efficiently internalized by cancer cells, and the loaded DOX could be successfully released under acidic intracellular environment. The above result shows that the core-shell nanocomposite could be a promising candidate for pH-responsive drug delivery system in the cancer therapy.
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ANALYTICAL CHEMISTRY
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2020
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IF 6.785
Yingna Bi,Huixia Di,Erzao Zeng,Qiang Li,Wenshuai Li,Jie Yang,Dingbin Liu
Intracellular pH is an important parameter that is highly associated with diverse physiological processes. The reliable measurement of pH values inside cells remains a formidable challenge because of the complexity of cytoplasm. Herein, we report a robust Prussian blue (PB)-caged pH-responsive surface-enhanced Raman scattering (SERS) probe for precisely mapping the dynamic pH values in live cells. The PB shell has a subnanoscale porous structure that allows only very small biospecies such as H+ or OH– to pass freely through the shell and react with the encased pH-responsive SERS probe, while physically resisting the entry of large biomolecules. This probe achieved unmatched detection linearity (R2 > 0.999) for pH measurements in diverse complex biological samples. Moreover, the nitrile (C≡N) in PB shows a sharp band in the cellular Raman-silent region, which serves as a background-free internal standard for accurate profiling of the probe distribution inside the cells. We applied the proposed probe to monitor the dynamic pH changes during cellular autophagy induced by different stimuli and thereby demonstrated that the PB-caged probe can reliably quantify subtle intracellular pH variations, providing an effective tool for revealing the relationship between abnormal intracellular pH and cellular functions.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Mengting Xie,Jiecheng Zhao,Xufeng Mai,Yan Chen,Wei Zhao,Ming Sun,Lin Yu,Hui-Juan Yu
A new ruthenium complex-based luminescent probe Ru-impa for Cu 2+ and pH detection has been synthesized and characterized. Ru-impa could rapidly and selectively detect Cu 2+ in aqueous solutions and the working pH ranges from weakly acidic to alkaline. The detection limit calculated using the S/N and S/B ratio was 24.7 nM and 3.4 μM, respectively. The test strips for practical detecting application were also prepared and the actual detection limit in drinking water was found to be 3 μM, which is lower than the WHO-guided drinking water limit (30 µM) and the upper limit of human serum free copper content (1.7–3.9 μM). Luminescence imaging study showed that Ru-impa could monitor Cu 2+ level fluctuation in the cells. In addition, Ru-impa also shows a sensitive on-off luminescence response when pH > 10, indicating that it can also be used as a pH sensor under extremely alkaline conditions.
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MICROPOROUS AND MESOPOROUS MATERIALS
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2022
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IF 5.876
You Liang,Sijin Wang,Huijuan Jia,Yijia Yao,Jiehui Song,Wenchao Yang,Yongsong Cao,Feng Zhu,Zhongyang Huo
The development of stimuli-responsive controlled release formulations is a promising strategy to enhance the utilization efficiency and reduce the environmental pollution of pesticides. In this work, a smart pH/redox/α-amylase triple stimuli-responsive pesticide delivery system was constructed by bonding carboxymethyl starch (CMS) with metal-organic frameworks (MIL-101(Fe)–NH 2 nanoparticles) which loaded with CAR ( [email protected] (Fe)-CMS nanoparticles). The results indicated that the loading capacity of [email protected] (Fe)-CMS nanoparticles for CAR was approximately 18.3% and the CMS attached on the surface of [email protected] (Fe)–NH 2 nanoparticles could protect CAR from photolysis effectively. Meanwhile, the coated CMS and metal-organic framework structure of the nanoparticles could be decomposed and consequently release pesticide active ingredients on demand when [email protected] (Fe)-CMS nanoparticles were metabolized by glutathione and α-amylase in the insect alkaline midgut. The bioactivity survey confirmed that [email protected] (Fe)-CMS nanoparticles had a longer duration in controlling Spodoptera frugiperda larvae compared to CAR suspension concentrate. In addition, the MIL-101(Fe)-CMS nanocarriers were safe for the growth of maize and could be used as fertilizer to promote the growth and development of crops. Thus, this smart stimuli-responsive pesticide-fertilizer all-in-one combination strategy has great potential in pest management.
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BIOMATERIALS
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2020
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IF 10.317
Xiaoting Gao,Shanshan Jiang,Chunying Li,Yunzhi Chen,Yifan Zhang,Peng Huang,Jing Lin
The development of tumor acidic microenvironment-responsive theranostic agents is a research hotspot. Herein, we developed highly photostable amphiphilic croconium dye-anchored red blood cell membrane vesicle (denoted as LET-5) for tumor pH-responsive near-infrared fluorescence (NIRF) and photoacoustic (PA) duplex imaging-guided photothermal therapy. In tumor acidic microenvironment , both NIRF and PA signals of LET-5 were significantly enhanced and the photothermal effect of LET-5 was activated. Notably, cell membrane-based vesicle with enhanced stability and long blood circulation significantly improved the tumor accumulation of croconium dye, thus achieving better therapeutic effect than free croconium dye. These findings provide a promising approach to construct amphiphilic dye-anchored cell membrane vesicle for cancer theranostics.
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Materials Science & Engineering C-Materials for Biological Applications
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2020
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IF 5.88
Xinli Li,Yunyun Chen,Xu Zhang,Yanbao Zhao
In this work, disulfide-bridged organic silica (OS) based nanocarriers were constructed for drug release. The broken of S S bonds in Si-O-Si skeleton would improve the degradation of Si-O-Si of OS carriers. The OS carriers have a central-radiated dendritic porous structure and a large specific surface area of 453.80 m 2 g −1 . The dextrin was selectively oxidized to dialdehyde dextrin (DAD) and then was modified on the surface of OS carriers by Schiff base bonds. Subsequently, cystamine (Cys) was linked with DAD to form DAD/Cys layer (OS-N=C-DAD/Cys) to seal the loaded drug. The DAD/Cys layer display the degradation performance of pH/GSH dual response The obtained OS-N=C-DAD/Cys carriers displayed low premature and the cumulative release was 6.5% under normal physiological conditions within 48 h. The Schiff base (-N=C-) structure in the DAD/Cys layer is also capable of monitoring acid-responsive drug release by fluorescence change. The prepared OS-N=C-DAD/Cys carriers and their degraded products have high biocompatibility.
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Materials Science & Engineering C-Materials for Biological Applications
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2018
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IF 5.08
Yixin Zhu,Yakun Ma,Yanli Zhao,Min Yang,Lingbing Li
In this paper, to achieve the targeted ability of anti-tumor drug doxorubicin (DOX), enhance the treatment effect and reduce the side effect, a novel pH-sensitive and charge-convertible prodrug nanogel was prepared. Firstly, cis -aconitic anhydride-doxorubicin prodrug (CAD) and Pluronic F127-chitosan-CAD (F127-CS-CAD) conjugates were synthesized. Then the DOX loaded polyion complex micelles (F127-CS-CAD/CAD) were prepared by self-assembling, thus CAD was incorporated into micelles via electrostatic interactions between electronegative CAD and positively charged F127-CS-CAD and hydrophobic interactions. Finally a pH-responsive charge-convertible copolymer, folic acid modified gelatin (Gel-FA) was shielded on the surface of micelles and the Gel-FA/F127-CS-CAD/CAD nanogel was formed, the charge-convertible capability was evaluated through changes of the morphology and Zeta potential under different pH value environment by transmission electron microscopy (TEM) and Zeta potential analyzer. And in vitro pH-dependent and two-phase drug release from nanogel was also evaluated. In vitro anti-tumor activity of Gel-FA/F127-CS-CAD/CAD nanogel was performed on HeLa cells and HepG2 cells to prove the strong cell toxicity of nanogels. Finally, the in vivo safety experiments showed that the nanogel achieved the reducing the toxic side effects of DOX significantly.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2022
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IF 9.965
Jingwen Ma,Juan Wang,Jian Liu,Xiang Li,Yuanqing Sun,Ruiying Li
The development of a low-cost and high-stability catalyst with Platinum-like electrocatalytic performance is significant for the hydrogen evolution reaction (HER) from water electrolysis. Herein, ultrafine ruthenium nanoparticles encapsulated in Nitrogen(N)-doped carbon anchored on graphene sheets are fabricated and utilized as HER electrocatalysts. Characterization results verify that the strong interaction between the N -doped carbon shell and the Ru core leads to charge redistributions, promoting Ru in a more active electron-rich state. Therefore, the catalyst exhibits excellent activity over a wide pH range, which only needs 16, 23 and 72 mV to drive a current density of 10 mA cm −2 in alkaline, acidic and neutral electrolytes, respectively. In addition, the N -doped carbon shell protects the Ru nanoparticles from aggregation and decomposition in the extreme electrolyte, contributing to the excellent stability.
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Journal of Materials Chemistry A
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2019
·Vol. 7
·期 28
·16793-16802
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IF 10.733
Lingyou Zeng,Kaian Sun,Yanju Chen,Zhi Liu,Yinjuan Chen,Yuan Pan,Ruiyu Zhao,Yunqi Liu,Chenguang Liu
Development of nonprecious, efficient, and stable electrocatalysts for overall water splitting under mild conditions is crucial but still a huge challenge for the renewable energy techniques. Herein, a facile hydrothermal and then selenization strategy is proposed to synthesize hierarchical nickel sulfoselenide (NiS2(1−x)Se2x) hollow/porous spheres with controllable composition for electrocatalytic water splitting in neutral media. By regulating the degree of selenization, the Se/S atomic ratio in the nickel sulfoselenides can be controlled to realize the tunable electronic structure of nickel to boost its intrinsic activity. Benefiting from their unique structural features and the anion doping effect, nickel sulfoselenides, especially with the chemical composition Ni(S0.5Se0.5)2, are discovered to exhibit high activity and stability toward both hydrogen and oxygen evolution reactions under neutral conditions. When using Ni(S0.5Se0.5)2 as a bifunctional electrode as both the anode and cathode in a neutral electrolyte, it demonstrates a durable activity for overall water splitting to drive 10 mA cm−2 at a cell voltage of merely 1.87 V, outperforming the noble metal-based Pt/C and IrO2 couple. This study not only offers a new type of promising earth-abundant electrocatalyst towards water electrolysis under benign conditions, but also highlights that anion doping engineering or composition control can be an elegant strategy to improve the electrochemical catalytic performance.
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Biomaterials Science
·
2018
·Vol. 6
·期 5
·1227-1237
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IF 5.831
Qianwen Li,Donghui Wang,Jiajun Qiu,Feng Peng,Xuanyong Liu
Hard tissue implant materials which can cause a suitable alkaline microenvironment are thought to be beneficial for stimulating osteoblast differentiation while suppressing osteoclast generation. To make the local pH around the interface between materials and cells controllable, we prepared a series of Mg–Fe layered double hydroxide (LDH) films on acid-etched pure titanium surfaces via hydrothermal treatment. By adjusting the Mg/Fe proportion ratio, the interlayer spacing of Mg–Fe LDHs was regulated, making their OH− exchange abilities adjustable, and this ultimately resulted in a microenvironment with a controllable pH value. In vitro experiments demonstrated that the Mg–Fe LDH film-modified titanium surface possessed good biocompatibility and osteogenic activity, especially the Mg–Fe LDH film with Mg/Fe proportion ratio of 4, which could form a suitable alkaline microenvironment for the growth and osteogenetic differentiation of stem cells. These results demonstrate the potential application of the prepared Mg–Fe LDH films in enhancing the osteogenesis of implant materials while providing a new way into the design of controllable alkaline environment.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2022
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IF 5.999
Weinan Li,HeXin Gong,Yuhan Fu,Jialin Sun,Yanhong Wang
Developing chemotherapy with nanoparticle-based prodrugs provides promising strategies for improving the safety and delivery of anti-cancer drugs therapeutics and effective cancer treatment. Herein, we developed a pH-sensitive prodrug delivery system (All-Trans-Retinoic Acid (ATRA) grafted poly (β-amino esters) (PBAE) copolymers , ATRA-g-PBAE) for delivery of ATRA with some physicochemical and biological properties. The in vitro release of ATRA-g-PBAE prodrug nanoparticles (PNPs) was sustained-release and pH-sensitive. The cytotoxicity and uptake of different preparations in vitro were evaluated on MCF-7 cells at pH 7.4 and 5.5. The carrier PBAE had no cytotoxicity, and ATRA-g-PBAE PNPs could significantly inhibit cell growth at pH 5.5. MCF-7 cells treated with Cy5.5 grafted PBAE (Cy5.5-PBAE) showed stronger fluorescence signals at pH 5.5. Meanwhile, ATRA-g-PBAE PNPs entered the cell via a clathrin-mediated endocytic pathway. Subsequently, PBAE protonation facilitated the escape of PNPs from the lysosome and released the drug. ATRA-g-PBAE seems promising as a novel pH-sensitive prodrug to overcome the limitations of ATRA for breast cancer therapy.
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