Nanoscale Research Letters
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2011
·Vol. 6
·期 1
·1-10
·
IF 2.56
Kai Wei,Xiaojun Xu,Ximing Pu,Zhenqing Hou,Qiqing Zhang
The evaluation of the toxicity of magnetic nanoparticles (MNPs) has attracted much attention in recent years. The current study aimed to investigate the cytotoxic effects of Fe 3 O 4 , oleic acid-coated Fe 3 O 4 (OA-Fe 3 O 4 ), and carbon-coated Fe (C-Fe) nanoparticles on human hepatoma BEL-7402 cells and the mechanisms. WST-1 assay demonstrated that the cytotoxicity of three types of MNPs was in a dose-dependent manner. G1 (Fe 3 O 4 and OA-Fe 3 O 4 ) phase and G2 (C-Fe) phase cell arrests and apoptosis induced by MNPs were detected by flow cytometry analysis. The increase in apoptosis was accompanied with the Bax over-expression, mitochondrial membrane potential decrease, and the release of cytochrome C from mitochondria into cytosol. Moreover, apoptosis was further confirmed by morphological and biochemical hallmarks, such as swollen mitochondria with lysing cristae and caspase-3 activation. Our results revealed that certain concentrations of the three types of MNPs affect BEL-7402 cells viability via cell arrest and inducing apoptosis, and the MNPs-induced apoptosis is mediated through the mitochondrial-dependent pathway. The influence potency of MNPs observed in all experiments would be: C-Fe > Fe 3 O 4 > OA-Fe 3 O 4 .
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Nature Sustainability
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2024
·
IF 25.7
Zhao Ziming,Li Tianyu,Zhang Changkun,Zhang Mengqi,Li Shenghai,Li Xianfeng
The growing global capacity for renewable energy generation necessitates the deployment of energy storage technologies with a combination of low cost, good performance and scalability. With these advantages, aqueous organic flow batteries have the potential to be the system of choice because they could store energy from organic redox-active molecules. Here we report naphthalene derivatives as organic redox-active molecules that exhibit high solubility (~1.5 M) and a stable redox-active framework with no obvious capacity decay over 40 days (50 Ah l −1 ) in an air atmosphere in flow batteries. We report a battery that runs smoothly even under continuous airflow without obvious capacity decay for ~22 days (more than 600 cycles). A series of spectral analyses and theoretical calculations reveal that the dimethylamine scaffolds improve the water solubility and protect the active centre, ensuring the stability of the molecules during the charge and discharge process. Owing to the success in kilogramme-scale molecular synthesis, pilot-scale stack expansion with notable cycling stability over 270 cycles (~27 days) is attained. The cost benefit evidenced by technoeconomic analysis together with the stability even under open-air conditions indicates the practical value of the present molecular system in grid-scale energy storage.
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Communications Biology
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2024
·Vol. 7
·期 1
·1-15
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IF 5.2
Zhang Wen,Cai Shuhui,Qin Lihong,Feng Yaru,Ding Menglei,Luo Zichen,Shan Jinjun,Di Liuqing
Aconiti Lateralis Radix Praeparata ( Fuzi in Chinese) is widely used in the clinical treatment of tumors. This study aims to explore the active fractions and underlying mechanisms of Fuzi in the treatment of non-small cell lung cancer (NSCLC). Fuzi alkaloids (FZA) is prepared and found to inhibit the growth of NSCLC both in vitro and in vivo significantly. A total of 53 alkaloids are identified in FZA by UPLC-Q-TOF-MS. Proteomics experiment show that 238 differentially expressed proteins regulated by FZA are involved in amino acid anabolism, pyrimidine metabolism and PI3K/Akt-mTOR signaling pathway. Metabolomics analyses identify 32 significant differential metabolites which are mainly involved in amino acid metabolism, TCA cycle and other pathways. Multi-omics research combined with molecular biological assays suggest that FZA might regulate glycolysis through PI3K/Akt-mTOR pathway to treat NSCLC. The study lays a foundation for the anti-cancer investigation of Fuzi and provides a possible scientific basis for its clinical application.
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MARINE BIOTECHNOLOGY
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2024
·
IF 2.6
Yang Qingsong,Yang Bing,Yang Bin,Zhang Wenqian,Tang Xiaoyu,Sun Huiming,Zhang Yanying,Li Jie,Ling Juan,Dong Junde
In the background of global warming, coral bleaching induced by elevated seawater temperature is the primary cause of coral reef degradation. Coral microbiome engineering using the beneficial microorganisms for corals (BMCs) has become a hot spot in the field of coral reef conservation and restoration. Investigating the potential of alleviating thermal stress by quorum quenching (QQ) bacteria may provide more tools for coral microbial engineering remediation. In this study, QQ bacteria strain Pseudoalteromonas piscicida SCSIO 43740 was screened among 75 coral-derived bacterial strains, and its quorum sensing inhibitor (QSI) compound was isolated and identified as 2,4-di-tert-butylphenol (2,4-DTBP). Then, the thermal stress alleviating potential of QQ bacteria on coral Pocillopora damicornis was tested by a 30-day controlled experiment with three different treatments: control group (Con: 29 °C), high temperature group (HT: 31 °C), and the group of high temperature with QQ bacteria inoculation (HTQQ: 31 °C + QQ bacteria). The results showed that QQ bacteria SCSIO 43740 inoculation can significantly mitigate the loss of symbiotic algae and impairment of photosynthesis efficiency of coral P. damicornis under thermal stress. Significant difference in superoxide dismutase (SOD) and catalase (CAT) enzyme activities between HT and HTQQ was not observed. In addition, QQ bacteria inoculation suppressed the coral microbial community beta-dispersion and improved the stability of microbial co-occurrence network under thermal stress. It was suggested that QQ bacteria inoculation can alleviate coral thermal stress via reshaping microbial interaction and maintain community stability of coral microbiome. This study provided new evidence for the probiotic function of QQ bacteria in corals, which shedding light on the development of new microbiological tools for coral reef conservation.
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CHINESE JOURNAL OF POLYMER SCIENCE
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2024
·
IF 4.1
Qiu Hong,Zhang Peng-Fei,Zhao Jun-Peng
Organocatalysis has shown special potency for simplifying the construction of complex polymer structures. We are reporting here a one-pot synthetic pathway using amine as a selectivity-switching agent in the two-component catalytic system consisting of triethylborane (Et 3 B) and a phosphazene base. We first modelled the interactions of a variety of amines with Et 3 B by density functional theory calculations. The results indicate that the aliphatic diamines comprising both primary and tertiary amino groups, capable of forming stable intramolecular hydrogen bonds, undergo the strongest complexation with Et 3 B. Accordingly, experimental results demonstrate that the addition of such amines promptly actuates the in situ selectivity switch from Lewis pair-catalyzed ring-opening polymerization (ROP) of epoxide (propylene oxide, n -butylglycidyl ether, or glycidyl phenyl ether) to organobase-catalyzed ROP of δ -valerolactone, allowing one-pot continuous synthesis of ether-ester type block copolymers. We thus exploited the noncovalent interaction between amine and Et 3 B to refine the catalyst switch strategy by exempting it from loading of extra catalyst.
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CYTOTECHNOLOGY
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2011
·Vol. 64
·期 2
·157-163
·
IF 1.277
Yi Yu,Wang Zhuhuan,Li Min,Zhu Keyin,Ying Guoqing
Monoclonal antibodies (McAbs) against chloramphenicol (CAP) were produced to detect CAP residues, which could be toxic and possesses a potential threat to human health. The CAP-BSA conjugate was obtained by bovine serum albumin (BSA) coupled with CAP, and used to immunize the mice. The splenocytes from the immunized mice were fused with mouse myeloma cells SP2/0 to form hybridoma, which may secrete McAbs against CAP. Hybridomas 1D 1 and 3G 12 secreting McAbs against CAP were obtained by screening. Ascites containing McAbs were prepared by injecting 1 x 10 6 cells of hybridoma 1D 1 and 3G 12 into the abdomen of mice. Protein A affinity chromatography was used to purify McAbs against CAP in a single chromatographic step with recovery yield above 80% and purity above 95% and full recovery of antibody activity. Experiments showed that McAb 3G 12 was highly specific for CAP and had no cross-reactivity with analogues which have a structure similar to CAP. The IC 50 value was 50.8 ng/mL.
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CHINESE JOURNAL OF POLYMER SCIENCE
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2011
·Vol. 29
·期 3
·288-295
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IF 0.478
Xu Gao-fei,Zhu Wei-pu
P(AA-MA) copolymers composed of acrylic acid and methyl acrylate with different molecular weights and sequence structures were synthesized by combination of ATRP and selective hydrolysis. These copolymers were used as membrane materials to separate benzene/cyclohexane mixture by pervaporation. The effects of molecular weight and sequence structure of the copolymers on the pervaporation performance were investigated in detail. For the random copolymers, the permeate flux decreased rapidly with the increasing of molecular weight. The separation factor was also influenced by the molecular weight, which was changed from no selectivity to cyclohexane selectivity with increasing the molecular weight. Contrarily, the block copolymer membrane showed good benzene selectivity with separation factor of 4.3 and permeate flux of 157 g/(m 2 h) to 50 wt% benzene/cyclohexane mixture.
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MICROCHIMICA ACTA
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2010
·Vol. 172
·期 3
·343-349
·
IF 2.648
Yin Huanshun,Zhou Yunlei,Ma Qiang,Liu Tao,Ai Shiyun,Zhu Lusheng
The electrochemical oxidation of guanosine-5′-monophosphate (GMP) was studied with a glassy carbon electrode modified with a composite made from graphene and multi-walled carbon nanotubes. GMP undergoes an irreversible oxidation process at an oxidation peak potential of 987 mV in phosphate buffer solution. Compared to other electrodes, the oxidation peak current of GMP with this electrode was significantly increased, and the corresponding oxidation peak potential negatively shifted, thereby indicating that the modified material exhibited electrochemical catalytic activity towards GMP. Chronocoulometry demonstrates that the material also effectively increases the surface area of the electrode and increases the amount of GMP adsorbed. Under the optimum conditions, the oxidation current is proportional to the GMP concentration in the range from 0.1 to 59.7 μM with a correlation coefficient of 0.9991. The detection limit is 0.025 μM (at S/N = 3).
We have developed an electrochemical method for sensitive determination of guanosine-5’-monophosphate (1) based on graphene and multi-walled carbon nanotubes modified glassy carbon electrode by amperometry.
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NATURE
·
2024
·Vol. 631
·期 8022
·783-788
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IF 50.5
Fang Zizheng,Mu Hongfeng,Sun Zhuo,Zhang Kaihang,Zhang Anyang,Chen Jiada,Zheng Ning,Zhao Qian,Yang Xuxu,Liu Feng,Wu Jingjun,Xie Tao
Three-dimensional (3D) printing has emerged as an attractive manufacturing technique because of its exceptional freedom in accessing geometrically complex customizable products. Its potential for mass manufacturing, however, is hampered by its low manufacturing efficiency (print speed) and insufficient product quality (mechanical properties). Recent progresses in ultra-fast 3D printing of photo-polymers 1 , 2 , 3 , 4 , 5 have alleviated the issue of manufacturing efficiency, but the mechanical performance of typical printed polymers still falls far behind what is achievable with conventional processing techniques. This is because of the printing requirements that restrict the molecular design towards achieving high mechanical performance. Here we report a 3D photo-printable resin chemistry that yields an elastomer with tensile strength of 94.6 MPa and toughness of 310.4 MJ m −3 , both of which far exceed that of any 3D printed elastomer 6 , 7 , 8 , 9 , 10 . Mechanistically, this is achieved by the dynamic covalent bonds in the printed polymer that allow network topological reconfiguration. This facilitates the formation of hierarchical hydrogen bonds (in particular, amide hydrogen bonds), micro-phase separation and interpenetration architecture, which contribute synergistically to superior mechanical performance. Our work suggests a brighter future for mass manufacturing using 3D printing.
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CELLULOSE
·
2025
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IF 4.8
Zhou Zixing,Tang Jiayi,Du Xiaosheng,Du Junhui,Deng Sha,Wang Haibo
Superhydrophobic cotton fabric is regarded as an accessible and efficient technology for oily wastewater cleanup because of high porosity, switch wettability, and low cost. However, the existing superhydrophobic fabrics cannot maintain durable superhydrophobicity during practical application due to poor action force between hydrophobic substance and fabric. Herein, a robust and durable fluorine-free superhydrophobic cotton fabric was successfully fabricated through a facile two-step modification process. Firstly, amino-functionalized SiO 2 nanoparticles were chemically bonded to the cotton fabric surface, creating a rough structure with peaks and valleys. Subsequently, the surface was coated with a layer of hydrophobic polydimethylsiloxane (PDMS) for surface modification, resulting in a superhydrophobic surface effective for the separation of oil–water mixtures. The as-prepared superhydrophobic cotton fabric (PDMS-SiO 2 @cot) presented superhydrophobic property with water contact angle reached 156.7°. Meanwhile, the prepared PDMS-SiO 2 @cot can effectively separate various oil–water mixtures with high separation efficiency (98.2–99.4%). Furthermore, the superhydrophobic cotton fabric demonstrated remarkable robustness by maintaining its hydrophobic characteristics after being subjected to a series of durability tests, including immersing into various solutions (acidic solution, alkaline solution, and organic solvents), and exposing to ultrasonication and mechanical stress (scraping, adhesive tape peeling, and sandpaper abrasion). In conclusion, the as-prepared superhydrophobic cotton fabric PDMS-SiO 2 @cot with excellent mechanical stability, chemical resistance, and self-cleaning properties shows considerable potential for oily water remediation. Graphical abstract
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JOURNAL OF MATERIALS SCIENCE
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2024
·Vol. 59
·期 27
·12447-12463
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IF 3.5
Gong Yanan,Xue Pan,Wang Xinyi,Ma Shaoshuai,Xu Xinhua
Developing hydrogel actuators with excellent driving performance and extended lifespan remains challenging. Ti 3 C 2 T x MXene, as a two-dimensional nanomaterial with a unique layered structure, has attracted widespread attention in flexible hydrogel actuators for its excellent optical absorption properties and tunable surface functionality. However, MXene faces difficulties in dispersion and is prone to oxidation, which significantly hinders the development and use of MXene-based hydrogel actuators. In this study, we fabricated a near-infrared light-driven hydrogel actuator with rapid photo responsiveness and antioxidative properties by incorporating modified MXene with antioxidant characteristics and the pore-forming agent polyvinyl alcohol into the poly( N -isopropylacrylamide) (PNIPAM) hydrogel system. We functionalized MXene nanosheets with (3-aminopropyl)triethoxysilane (APTES), effectively enhancing antioxidative properties, preventing structural degradation caused by spontaneous oxidation, and improving surface properties. This enhanced the dispersion stability of MXene in the system and extended its lifespan from 7 days to over two weeks. The hydrophilic polyvinyl alcohol chains served as drainage channels during hydrogel contraction, imparting the hydrogel with rapid driving capabilities (127.1° s −1 ). Additionally, leveraging the fast response characteristics, we designed an octopus-inspired light-driven soft swimmer and gripper. This work provides novel insights into the application of intelligent responsive hydrogels in biomimetic and practical scenarios. Graphical abstract
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-13
·
IF 14.7
Tang Si,Liu Yaqing,Zhu Jianming,Cheng Xueyu,Liu Lu,Hammerschmidt Katrin,Zhou Jin,Cai Zhonghua
Understanding how organisms have adapted to persist in unpredictable environments is a fundamental goal in biology. Bet hedging, an evolutionary adaptation observed from microbes to humans, facilitates reproduction and population persistence in randomly fluctuating environments. Despite its prevalence, empirical evidence in microalgae, crucial primary producers and carbon sinks, is lacking. Here, we report a bet-hedging strategy in the unicellular microalga Haematococcus pluvialis . We show that isogenic populations reversibly diversify into heterophenotypic mobile and non-mobile cells independently of environmental conditions, likely driven by stochastic gene expression. Mobile cells grow faster but are stress-sensitive, while non-mobile cells prioritise stress resistance over growth. This is due to shifts from growth-promoting activities (cell division, photosynthesis) to resilience-promoting processes (thickened cell wall, cell enlargement, aggregation, accumulation of antioxidant and energy-storing compounds). Our results provide empirical evidence for bet hedging in a microalga, indicating the potential for adaptation to current and future environmental conditions and consequently conservation of ecosystem functions.
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