CARBOHYDRATE POLYMERS
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2022
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IF 10.723
Miao Miao,Lan Mu,Shaomei Cao,Yuhuan Yang,Xin Feng
Tetracycline (TC) residues have been noted as an important class of emerging contaminants in the environment, thus fast, straightforward, highly sensitive detection method is becoming an issue that must be addressed. Herein, a novel CDs@ZIF-8 sensing material was fabricated by encapsulating luminescent carbon dots (CDs) into the metal-organic framework (ZIF-8) to achieve highly luminescent porous composites. Furthermore, to avoid the secondary contamination caused by powders, a shapable CDs@ZIF-8/CS film sensor as a portable disposable test strip with dual-function of TC detection and adsorption was designed by crosslinking CDs@ZIF-8 with chitosan (CS). The as-prepared CDs@ZIF-8/CS hybrid film exhibited the high sensitivity and selectivity for TC fluorescence detection and rapid decontaminate capability, as well as high optical transmittance and robust mechanical property, which were essential for the practical sensing application. This paper-based dual-functional luminescent sensor exhibits promising practicability for TC detection and contaminant removal in pharmaceutical analysis, food safety and water treatment.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Jie Song,Chaoqun Zhang,Shaowen Kong,Fangyu Liu,Wenju Hu,Feng Su,Suming Li
This work aimed to develop a novel chitosan based metal-organic polyhedrons (MOPs)/enzyme hybrid hydrogel with superior antimicrobial properties in wound healing treatment. Hybrid hydrogel was prepared by crosslinking glucose oxidase (GOx), vanadium metal-organic polyhedrons (VMOP-2) and chitosan using glutaraldehyde as crosslinker. The formed GVCS hydrogel was characterized by using various techniques, including FTIR, SEM, XPS , TGA and EDX . Data show that GVCS hydrogel was successfully obtained with uniform distribution of GOx and VMOP-2 in the hydrogel structure. Antibacterial tests show that GVCS hydrogel exhibits better bactericidal effect on both gram-negative bacteria ( S. aureus ) and gram-positive bacteria ( E. coli ) compared to other hydrogel samples because of its hydroxyl radicals generation capacity in the presence of glucose. MTT assay shows that the hydrogel presents good cell compatibility. In vivo experiments using an infected wound model indicate that GVCS hydrogel can effectively facilitate wound healing. Therefore, chitosan based MOPs/enzyme hybrid hydrogel could be most promising for antibacterial therapy in clinical applications.
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JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Ting Pan,Huanhuan Chen,Xiang Gao,Zeyu Wu,Yingwang Ye,Yizhong Shen
Bacterial biofilms have evoked worldwide attention owing to their serious threats to public health, but how to effectively eliminate bacterial biofilms still remains great challenges. Here, we rationally designed a novel and vigorous chitosan grafted Fe-doped-carbon dots ( [email protected] /CDs) as an efficient artificial nanozyme to combat rigid bacterial biofilms through the selective activation of Fenton-like reaction-triggered peroxidase-like catalytic activity and the synergistic antibacterial activity of CS. On the one hand, the peroxidase-like catalytic activity made [email protected] /CDs catalyze H 2 O 2 for producing hydroxyl radicals (•OH), resulting in efficient cleavage of extracellular DNA (eDNA). On the other hand, CS was capable of binding with the negatively charged cell membrane through electrostatic interaction, changing the cell membrane permeability and causing cell death within bacterial biofilms. Based on their synergistic effects, the fragments of bacterial biofilm and exposed bacteria were persistently eradicated. Remarkably, [email protected] /CDs-based nanozyme not only enabled the effective destroying of gram-positive Staphylococcus aureus ( S. aureus ) biofilms, but also completely eliminated gram-negative Pseudomonas aeruginosa ( P. aeruginosa ) biofilms, showing great potential as a promising anti-biofilm agent against bacteria biofilms. This proposed synergistic strategy for bacterial biofilm eradication might offer a powerful modality to manage of bacterial biofilm fouling in food safety and environmental protection.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Yang Xu,Na Liang,Jiyang Liu,Xianfeng Gong,Pengfei Yan,Shaoping Sun
In this study, cetyl 4-formylbenzoate alkyl and 4-(2-hydroxyethoxy) benzophenonesalicylaldazide modified biotinylated chitosan (CS-BT-HBS-CB) featured with aggregation-induced emission (AIE) characteristic, active tumor-targeting ability and pH-responsive drug release property was designed and synthesized. The polymer was fabricated by introducing hydrophobic segment, tumor targeting ligand, acid-sensitive bond and AIE fluorophore to the backbone of chitosan. Due to its amphiphilicity, the polymer could self-assemble into micelles and encapsulate paclitaxel (PTX) to form PTX-loaded CS-BT-HBS-CB micelles. The mean size of the micelles was 167 nm, which was beneficial to the EPR effect . Moreover, with the help of above functional groups, the micelles exhibited excellent AIE effect, triggered drug release behavior by acidic condition, selective internalization by MCF-7 cells and excellent cellular imaging capability. In vivo studies revealed that the PTX-loaded CS-BT-HBS-CB micelles could enhance the antitumor efficacy with low systemic toxicity. This micellar system would be a potential candidate for cancer therapy and bioimaging.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
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IF 6.056
Chun Yang,Jia-Hao Lu,Mei-Ting Xu,Xin-Chi Shi,Zhi-Wei Song,Tang-Min Chen,Daniela D. Herrera-Balandrano,Yun-Jiao Zhang,Pedro Laborda,Mahdi Shahriar,Su-Yan Wang
Strawberries are a widely consumed fruit that contains a variety of antioxidant metabolites. However, postharvest strawberries are highly perishable and susceptible to a variety of fungal pathogens. In this study, a chitosan-based coating containing turmeric (TU) and green tea (GT) extracts was developed and evaluated for the preservation of strawberries. Both TU and GT extracts contained high phenolic contents (>5.8 g kg −1 dry weight), but only TU extract showed strong antifungal activity. SEM observations revealed that high concentrations of extract (3–5% v/v ) caused the formation of agglomerates within the films, reduced elongation at break and increased tensile strength. The postharvest treatment of strawberries with chitosan coating containing TU extract inhibited the proliferation of Botrytis cinerea during 7 d of storage at 20 °C, whereas the coating containing GT extract extended the antioxidant properties of postharvest strawberries from 4 to 8 d at 20 °C. Sensory analysis indicated that the coatings did not significantly affect flavor and taste. Overall, TU extract was used for the first time for food packaging purposes and proved to be a great complement to the antioxidant properties of GT to extend the shelf-life of postharvest strawberries.
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Polymers
·
2022
·Vol. 14
·期 11
·2133
·
IF 4.967
Ziwei Xia,Zhinan Fu,Li Li,Enguang Ma,Liang Sun,Qinyu Ma,Xuhong Guo
Metal coordination-driven nanocomplexes are known to be responsive to physiologically relevant stimuli such as pH, redox, temperature or light, making them well-suited for antitumor drug delivery. The ever-growing demand for such nanocomplexes necessitates the design of a scalable approach for their production. In this study, we demonstrate a novel coordination self-assembly strategy, termed flash nanocomplexation (FNC), which is rapid and efficient for the fabrication of drug-loaded nanoparticles (NPs) in a continuous manner. Based on this strategy, biocompatible chitosan (CS) and Cu2+can be regarded anchors to moor the antitumor drug (curcumin, Cur) through coordination, resulting in curcumin-loaded chitosan nanocomplex (Cur-loaded CS nanocomplex) with a narrow size distribution (PDI < 0.124) and high drug loading (up to 41.75%). Owing to the excellent stability of Cur-loaded CS nanocomplex at neutral conditions (>50 days), premature Cur leakage was limited to lower than 1.5%, and pH-responsive drug release behavior was realized in acidic tumor microenvironments. An upscaled manufacture of Cur-loaded CS nanocomplex is demonstrated with continuous FNC, which shows an unprecedented method toward practical applications of nanomedicine for tumor therapy. Furthermore, intracellular uptake study and cytotoxicity experiments toward H1299 cells demonstrates the satisfied anticancer efficacy of the Cur-loaded CS nanocomplex. These results confirm that coordination-driven FNC is an effective method that enables the rapid and scalable fabrication of antitumor drugs.Keywords:chitosan;flash nanocomplexation;coordination-driven;curcumin
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Food Chemistry-X
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2022
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IF 6.443
Ben Niu,Hangjun Chen,Weijie Wu,Xiangjun Fang,Honglei Mu,Yanchao Han,Haiyan Gao
Most of the current research only explored the loading of an active substance in active packaging. In this study, cinnamaldehyde essential oil (CEO) and chlorogenic acid (CA) were co-encapsulated in chitosan (CS) nanoparticles based Pickering emulsion. The morphology and wettability of CS-CA particles were determined. In addition, physicochemical characterizations and stability of the Pickering emulsion were also investigated. Results showed that the wettability of nanoparticles was improved with increasing the ratios of CS to CA, which is helpful to stabilize the emulsion. CEO Pickering emulsion was stabilized by CS-CA nanoparticles and CEO emulsion showed the best stability by using CS-CA nanoparticles with the ratios of CS to CA 1:0.75 with the minimum creaming index value of 26.5 ± 4.6% after 5 days of storage. These overall results presented in this work demonstrate, for the first time, the potential of Pickering emulsion for the co-encapsulation of water-soluble and water-insoluble ingredients.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2022
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IF 5.999
Mei-Yan Bai,Qi Zhou,Jie Zhang,Ting Li,Jun Cheng,Qun Liu,Wen-Rong Xu,Yu-Cang Zhang
Chitosan (CS) is becoming increasingly popular in food packaging due to its natural degradability and great film-forming properties. Nevertheless, its poor antibacterial properties and inadequate antioxidant properties prevent it from being used effectively. In this study, β-cyclodextrin-epichlorohydrin (β-CD-EP) oligomers were prepared and encapsulated with natural essential oils cinnamaldehyde and thymol , and then the inclusion complexes (IC) were incorporated into chitosan in various contents to afford a series of CS-IC composite films . The impacts of IC on the morphological, mechanical, thermal, and water resistance properties, antioxidant and antibacterial activities of chitosan films, as well as the loading and sustained release behavior of IC, were thoroughly examined. The results turned out that the essential oils were well-loaded with high encapsulation efficiency and showed a significant slow-release effect. It was also found that the tensile strength and the elongation at break decreased with increasing IC contents, while the thermal stability was enhanced. The incorporation of IC dramatically promoted the antioxidant and antibacterial properties of the chitosan films towards Gram-positive bacteria. Based on our findings, chitosan films containing essential oils-loaded β-CD-EP oligomers may serve as an effective food packaging material.
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Biomedical Materials
·
2022
·Vol. 17
·期 3
·035006
·
IF 4.103
Haibo Wang,Ao Dong,Kun Hu,Weiwei Sun,Jundong Wang,Lu Han,Lixin Mo,Luhai Li,Wei Zhang,Yan Guo,Li Zhu,Fuzhai Cui,Yen Wei
Poly L-lactic acid (PLLA) is a non-toxic, biocompatible degradable polymer material with excellent mechanical properties after moulding. However, it faces challenges in the use of biomedical materials because of its intolerance to bacteria. Here, we use an easy-to-operate method to prepare a composite multilayer membrane: PLLA membrane was used as substrates to assemble positively charged chitosan and negatively charged Ag@MXene on the surface using the layer-by-layer (LBL) method. The assembly process was detected by fluorescein isothiocyanate-labelled chitosan and the thickness of the coating multilayer was also detected as 210.0 ± 12.1 nm for P-M membrane and 460.5 ± 26.5 nm for P-Ag@M membrane. The surface self-assembled multilayers exhibited 91.27% and 96.11% growth inhibition ratio against Escherichia coli and Staphylococcus aureus strains under 808 nm near-infrared laser radiation with a synergistic photothermal antibacterial effect. Furthermore, best biocompatibility of P-M and P-Ag@M membranes compare to PLLA membrane motivated us to further explore its application in biomedical materials.
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Materials Today Communications
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2022
·
IF 3.662
Lei Nie,Jie Li,Guoqi Lu,Xiaoyan Wei,Yaling Deng,Shuang Liu,Shengping Zhong,Qimin Shi,Ruixia Hou,Yi Sun,Constantinus Politis,Lihong Fan,Oseweuba Valentine Okoro,Amin Shavandi
Temperature-responsive hydrogels exhibit great potentials to encapsulate cells for 3D bioprinting and tissue engineering applications; however, cell aggregation and the hydrogel stiffness determine cell fate and limit its application. Here, we developed a new temperature-responsive hydrogel with interpenetrating polymer networks using poly (N-isopropylacrylamide) (pNiPAM) and hydroxyethyl-chitosan (HECS) with the incorporation of dithiol-modified graphene oxide nanosheets (t-GO). The fabricated hydrogel showed excellent cytocompatibility toward human bone marrow mesenchymal stem cells (hBMSCs), and as expected, the lower critical solution temperature (LCST) could be regulated by changing the weight ratio of pNiPAM/HECS/t-GO. Finally, hBMSCs could be directly encapsulated in the hydrogel at a low temperature (around 20 °C), after then, the cell-laden hydrogel was formed above LCST and displayed high cell viability after 3D culturing. The results indicate that the new pNiPAM/HECS/t-GO hydrogel has the potential to serve as a cell carrier for drug delivery and tissue engineering applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Longquan Xiao,Shuai Kang,Molazi Lapu,Peng Jiang,Xinhui Wang,Dayu Liu,Jing Li,Mingxue Liu
In this research, the common microorganisms in chilled meat were used as the targeted antibacterial objects. Chitosan, pullulan, and carvacrol were chosen to prepare the edible antibacterial film. The effects of different concentrations of carvacrol on the microstructure, physical properties and antibacterial properties of the films were investigated. The results showed that the antibacterial activity of chitosan/pullulan film (CS/PU) was unsatisfactory, when carvacrol was added, the antibacterial activity of the chitosan/pullulan/carvacrol film (CS/PU/CAR) improved significantly ( p < 0.05 ), and the water vapor permeability (WVP) of the CS/PU/CAR decreased significantly ( p < 0.05 ). When the carvacrol concentration was higher than 1.25% (w/v), the tensile strength and percentage elongation at break of the CS/PU/CAR increased significantly ( p < 0.05 ), and the CS/PU/CAR exhibited satisfying antibacterial activity against the common bacteria in chilled meat such as Pseudomonas fluorescens , Listeria monocytogenes , Escherichia coli , Pseudomonas putida , Enterobacter cloacae , and Staphylococcus aureus . Finally, the CS/PU/CAR film was applied to the preservation of chilled goat meat and extended the shelf life of goat meat to more than 15 days. These results suggested that the targeted CS/PU/CAR film can be used as biodegradable films for the active packaging of chilled meat.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Yuhui Sheng,Xingchen Sun,Jingtian Han,Wei Hong,Jie Feng,Shuyang Xie,Youjie Li,Fangwei Yan,Keke Li,Baocheng Tian
An N -acetylcysteine functionalized chitosan oligosaccharide-palmitic acid conjugate (NAC-COS-PA) with bioadhesive and permeation promoting properties was synthesized to enhance transocular drug delivery. Flurbiprofen (FB) loaded self-assembled NAC-COS-PA nanomicelles (NAC-COS-PA-FB) were prepared and the drug loading was 7.35 ± 0.32%. Human immortalized corneal epithelial (HCE-T) cell cytotoxicity and hen's egg test-chorioallantoic membrane assays confirmed that the conjugate had good biocompatibility. The transportation efficiency of coumarin-6 (C6) loaded nanomicelles in the HCE-T cell monolayer was approximately 1.97 times higher than that of free C6. Decreased intracellular Ca 2+ concentration and cell membrane potential , increased cell membrane fluidity , and reversible changes in the F-actin cytoskeleton are presumed to be responsible for the enhanced drug permeation. NAC-COS-PA exhibited strong binding capacity with mucin and rabbit eyeball. In vivo pharmacokinetics indicated that the area under the curve (AUC 0 – 6 h ) and the maximum concentration (C max ) of NAC-COS-PA-FB were approximately 1.92 and 2.44 times that of the FB solution, respectively. NAC-COS-PA-FB demonstrated the best in vivo anti-inflammatory efficacy compared to unfunctionalized nanomicelles (COS-PA-FB) and FB solution. Consequently, NAC-COS-PA appears to be a promising bioadhesive carrier for ophthalmic delivery.
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