CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2023
·Vol. 39
·期 3
·516-524
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IF 3.1
Cheng Xue,Jiang Xinshu,Liu Liquan,Huang Jun
Emerging contaminants(ECs) have aroused great concerns due to their widespread presence as well as their potential health and ecological risks. Currently monitoring data about ECs in Chinese rural areas are still limited. In this study, Taige Canal basin was selected as a typical rural area for a pilot study. The occurrence of ECs, including pharmaceutical and personal care products(PPCPs), pesticides, plasticizers and flame retardants, in surface water and aquaculture ponds, as well as in wastewater treatment facilities, was comprehensively investigated. The effectiveness of rural decentralized wastewater treatment facilities and centralized wastewater treatment plant(WWTP) in removing ECs was also analyzed. Among 126 commonly used pesticides for target analysis, 25 compounds were detected in surface water with high frequency of >80%. Besides, 55 PPCPs, 21 pesticides and 18 other industrial products were identified by non-target analysis using high resolution mass spectrometry(HRMS). Totally 18 compounds were detected in surface water with a frequency of >60% and 6 of them were found in all samples, including irbesartan, valsartan, propazine, buprofezin, bis(2-ethylhexyl) phthalate(DEHP) and tri-(2-chloroisopropyl) phosphate(TCPP), with mean concentrations of 3022, 195, 125, 218, 3738 and 545 ng/L, respectively. There are 29 compounds(11 PPCPs, 8 pesticides and 10 other industrial products) detected in water samples from the aquaculture ponds, of which 13 compounds were found in all three ponds. The removal efficiencies of ECs in wastewater treatment facilities are generally limited, except valsartan(82%–86%), nobiletin(100%) and diisodecylphthalate(100%) were found to be significantly removed.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 10
·8682
·
IF 5.6
Zijiao Zhang,Juhan Song,Seung-Hee Kwon,Zhao Wang,Suk-Gyun Park,Xianyu Piao,Je-Hwang Ryu,Nacksung Kim,Ok-Su Kim,Sun-Hun Kim,Jeong-Tae Koh
There has been increasing interest in adjunctive use of anti-inflammatory drugs to control periodontitis. This study was performed to examine the effects of pirfenidone (PFD) on alveolar bone loss in ligature-induced periodontitis in mice and identify the relevant mechanisms. Experimental periodontitis was established by ligating the unilateral maxillary second molar for 7 days in mice (n = 8 per group), and PFD was administered daily via intraperitoneal injection. The micro-computed tomography and histology analyses were performed to determine changes in the alveolar bone following the PFD administration. For in vitro analysis, bone marrow macrophages (BMMs) were isolated from mice and cultured with PFD in the presence of RANKL or LPS. The effectiveness of PFD on osteoclastogenesis, inflammatory cytokine expression, and NF-κB activation was determined with RT-PCR, Western blot, and immunofluorescence analyses. PFD treatment significantly inhibited the ligature-induced alveolar bone loss, with decreases in TRAP-positive osteoclasts and expression of inflammatory cytokines in mice. In cultured BMM cells, PFD also inhibited RANKL-induced osteoclast differentiation and LPS-induced proinflammatory cytokine (IL-1β, IL-6, TNF-a) expression via suppressing the NF-κB signal pathway. These results suggest that PFD can suppress periodontitis progression by inhibiting osteoclastogenesis and inflammatory cytokine production via inhibiting the NF-κB signal pathway, and it may be a promising candidate for controlling periodontitis.Keywords:pirfenidone;periodontitis;osteoclast differentiation;alveolar bone loss;inflammation;NF-κB pathway
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Sanlei Xie,Yuehong Yue,Guoyang Xu
To prevent the hazard concerns of amantadine (AMD) on humans, a sensitive and reliable detection method is urgently required. Herein, we reported a one-pot method in situ synthesis of carbon nanoparticles (CNPs) exhibited yellow color and green fluorescence. 4-Aminophenyl phosphate was catalyzed by alkaline phosphatase (ALP) to ρ-aminophenol (AP), and induced polymerization with diethylenetriamine (DETA) under a mild condition, which promotes in situ formation of CNPs with excellent photoluminescence stability. According to ALP-triggered specific catalytic reaction and in situ formed CNPs as signal tags, we proposed a dual-signal immunoassay for colorimetric and fluorometric monitoring AMD residues in poultry foodstuffs. Under an optimized detection parameter, the linear range and detection limit corresponded to colorimetric and fluorometric were 0.03 to 200 ng g −1 and 0.03 to 200 ng g −1 , 0.047 ng g −1 and 0.060 ng g −1 , respectively. The convincing performance of our established in-situ dual-readout sensing platforms opened a promising horizon for monitoring AMD residues in poultry productions.
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MOLECULAR PHARMACEUTICS
·
2023
·
IF 4.9
Yaxin Cheng,Jinsong Tao,Yaqi Zhang,Long Xi,Run Han,Meng Xu,Simon Ming-Yuen Lee,Wei Ge,Yong Gan,Ying Zheng
Mesoporous silica nanoparticles (MSNs) are widely used in the biomedical field because of their unique and excellent properties. However, the potential toxicity of different shaped MSNs via injection has not been fully studied. This study aims to systematically explore the impact of shape and shear stress on the toxicity of MSNs after injection. An in vitro blood flow model was developed to investigate the cytotoxicity and the underlying mechanisms of spherical MSNs (S-MSN) and rodlike MSNs (R-MSN) in human umbilical vein endothelial cells (HUVECs). The results suggested that the interactions between MSNs and HUVECs under the physiological flow conditions were significantly different from that under static conditions. Whether under static or flow conditions, R-MSN showed better cellular uptake and less oxidative damage than S-MSN. The main mechanism of cytotoxicity induced by R-MSN was due to shear stress-dependent mechanical damage of the cell membrane, while the toxicity of S-MSN was attributed to mechanical damage and oxidative damage. The addition of fetal bovine serum (FBS) alleviated the toxicity of S-MSN by reducing cellular uptake and oxidative stress under static and flow conditions. Moreover, the in vivo results showed that both S-MSN and R-MSN caused cardiovascular toxicity in zebrafish and mouse models due to the high shear stress, especially in the heart. S-MSN led to severe oxidative damage at the accumulation site, such as liver, spleen, and lung in mice, while R-MSN did not cause significant oxidative stress. The results of in vitro blood flow and in vivo models indicated that particle shape and shear stress are crucial to the biosafety of MSNs, providing new evidence for the toxicity mechanisms of the injected MSNs.
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Thoracic Cancer
·
2023
·Vol. 14
·期 14
·1276-1285
·
IF 2.9
Shuanghu Wang,Mengming Xia,Yu Wang,Zebei Lu,Peiwu Geng,Dapeng Dai,Yunfang Zhou,Qingjun Wu
Background As a pan-HER tyrosine kinase inhibitor with a promising application prospect, poziotinib is likely to be coadministered with Schisandrins in clinical treatment due to its anticancer activities. Methods Eighteen Sprague–Dawley rats were randomly divided into three groups: Schisandrin A group and Schisandrin B group (20 mg/kg daily for 1 week), and control group (vehicle). On day 8, poziotinib (2 mg/kg) was administered by oral gavage 30 min later. An in vitro study was developed to identify the possible mechanisms of Schisandrins on poziotinib metabolism. All analytes were detected by UPLC/MS–MS, and molecular docking was performed by AutoDock Tools. Results When rats were preadministered with Schisandrin A, AUC (0−∞) and Cmax of poziotinib were obviously increased by 0.79- and 1.17-fold, whereas the Vz/F and CLz/F values were dramatically decreased. The results in Schisandrin B group presented similarly. Both Schisandrin A and Schisandrin B were mixed inhibitors of poziotinib in RLMs, and Schisandrin B showed stronger inhibitory activity with IC 50 values of 2.55 μM for M1 and 6.97 μM for M2. Molecular docking analysis demonstrated that Schisandrin A and Schisandrin B exhibited a strong binding ability towards CYP2D6 as compared to CYP3A4. Conclusion All results provided the direct evidence of the pharmacokinetic drug–drug interactions (DDIs) between Schisandrin and poziotinib. Thus, particular attention should be paid when poziotinib is taken together with Schisandrins in clinical practice.
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PHYTOTHERAPY RESEARCH
·
2023
·
IF 7.2
Li Liang,Weiliang Sun,Xiaoxuan Wei,Li Wang,Huaqiang Ruan,Junchuan Zhang,Suyan Li,Bi Zhao,Mengshi Li,Zhengwen Cai,Jie'an Huang
Chinese herb Radix sophorae tonkinensis extract oxymatrine shows anticancer effects. This study evaluated the role of oxymatrine in colorectal cancer (CRC) and the underlying molecular events in vitro and in vivo. CRC cells were treated with different doses of oxymatrine to assess cell viability, reactive oxygen species production, gene expression, and gene alterations. Meanwhile, mouse xenograft and liver metastasis models were used to assess the effects of oxymatrine using histology examination, transmission electron microscopy, and Western blot, respectively. Our results showed that oxymatrine treatment triggered CRC cell mitophagy to inhibit CRC cell growth, migration, invasion, and metastasis in vitro and in vivo. At the gene level, oxymatrine inhibited LRPPRC to promote Parkin translocation into the mitochondria and reduce the mitophagy-activated NLRP3 inflammasome. Thus, oxymatrine had an anticancer activity through LRPPRC inhibition, mitophagy induction, and NLRP3 inflammasome suppression in the CRC cell xenograft and liver metastasis models. In conclusion, the study demonstrates the oxymatrine anti- CRC activity through its unique role in regulating CRC cell mitophagy and NLRP3 inflammasome levels in vitro and in vivo.
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WATER RESEARCH
·
2022
·
IF 13.4
Yuefei Song,Yajuan Li,Xiaomei Chen,Chunchun Meng,Saifei Ma,Tiemei Li,Kai Jiang,Chun Hu
Bifunctional photocatalytic nanofiltration (PNF) membrane is increasingly concerned in practical micro-polluted water purification , but there are still several bottlenecks that inhibit its practicality. In this context, the feasibility of a novel metal-free and visible light-responsive surface-anchored PNF membrane for simultaneously removing target antibiotics in real sewage effluent in a continuous dynamic process was explored. The results showed that the optimal PNF-4 membrane was expectedly consisted of an inside tight sub-nanopore structured separation layer and an outside thinner, smoother, super hydrophilic mesoporous degradation layer, respectively. Consequently, the activated PNF-4 membrane could synergistically reduce trimethoprim and sulfamethoxazole concentrations to below two orders of magnitude, accompanying with almost constant high water permeability, suggesting that the hydrophilic modification of the mesoporous degradation layer basically offsets its inherent hydraulic resistance. Also, after repeating the fouling-physical rinsing process three times lasted for 78 h, only sporadic adherent contaminants remained onto the top surface, together with the minimal total and irreversible fouling ratios (as low as 7.2% and 1.2%, respectively), strongly demonstrated that PNF-4 membrane displayed good self-cleaning performance. Undoubtedly, this will significantly reduce its potential cleaning frequency and maintenance cost in long-term operation. Meanwhile, the acute and chronic biotoxicities of its permeate to Virbrio qinghaiensis sp. -67 were also reduced sharply to 2.22% and 0.45%, respectively. All of these evidences suggest that the dual functions of PNF-4 membrane are synergetic in an uninterrupted permeating process. It will provide useful insights for continuously enhancing the practicality and effectiveness of PNF membrane in actual micro-polluted water purification scenarios.
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ANALYST
·
2022
·Vol. 148
·期 4
·752-761
·
IF 5.227
Xintong Lu,Luoyu Ding,Haohan Song,Wenxin Yu,Chaoqing Dong,Jicun Ren
Matrix metalloproteinase-9 (MMP-9) plays an important role in tumor progression. It is of great significance to establish a sensitive in situ assay strategy for MMP-9 activity in single living cells. Here a novel in situ single molecule spectroscopy method based on the fluorescence correlation spectroscopy (FCS) technique was proposed for measuring the MMP-9 activity at different locations within single living cells, using a fluorescent specific peptide and a reference dye as dual probes. The measurement principle is based on the decrease of the ratiometric translational diffusion time of dual probes in the detection volume due to the peptide cleavage caused by MMP-9. The peptide probe was designed to be composed of an MMP-9 cleavage and cell-penetrating peptide sequence that was labeled with a fluorophore and conjugated with a streptavidin (SAV) molecule. The ratiometric translational diffusion time was used as the measurement parameter to eliminate the effect of intracellular uncertain viscosity. The linear relationship between the ratiometric diffusion time and MMP-9 activity was established, and applied to the determination of enzymatic activity in cell lysates as well as the evaluation of the inhibitory effects of different inhibitors on MMP-9. More importantly, the method was successfully used to dynamically determine MMP-9 activity in single living cells or under the stimulation with phorbol 12-myristate 13-acetate (PMA) and inhibitors.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
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IF 5.8
Yong-Qiang Xu,Liu-Yuan Zang,Hai-Yu Gao,Jin Peng,Dong-Yun Zheng,Chao Liu,Xiao-Jun Liu,Dong-Bing Cheng,Chun-Nan Zhu
Gd 3+ -doped quantum dots (QDs) have been widely used as small-sized bifunctional contrast agents for fluorescence/magnetic resonance (FL/MR) dual-modality imaging. However, Gd 3+ doping will always compromise the FL of host QDs. Therefore, balancing the Gd 3+ doping and the optical properties of QDs is crucial for constructing high-performance bifunctional nanoprobes . Additionally, most paramagnetic QDs are synthesized in the organic phase and need to be transferred to the aqueous phase for bioimaging. Herein, ingeniously designed shell-doped Cu-In-S/ZnS:Gd 3+ QDs have been prepared in the aqueous phase. It has been demonstrated that isolating paramagnetic Gd 3+ from fluorescent Cu-In-S core via doping Gd 3+ into ZnS shell not only avoided the decrease of FL quantum yield (QY), but also ensured the water accessibility of paramagnetic Gd 3+ ions, by which the FL QY and r 1 relaxivity of Cu-In-S/ZnS:Gd 3+ QDs achieved as much as 15.6% and 15.33 mM -1 ·s -1 , respectively. These high-performance QDs with excellent stability, low biotoxicity, and good tumor permeability were successfully applied for in vivo tumor FL/MR dual-modality imaging, and have shown significant potential in the precision detection and diagnosis of diseases.
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Environmental Science-Nano
·
2022
·Vol. 10
·期 2
·567-580
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IF 9.473
Yuefei Song,Chunchun Meng,Xiaomei Chen,Yajuan Li,Saifei Ma,Lingjie Zhang,Jinran Wang,Kai Jiang
Bifunctional photocatalytic nanofiltration (PNF) membranes have gradually become one of the research hotspots in the field of micropolluted water purification. However, their usability still needs to be greatly improved. To this end, a novel surface-anchored PNF membrane was developed for synchronously degrading and separating target antibiotics in actual sewage effluent under continuous flow-through. As expected, experimental results demonstrated that the optimized PNF-2 membrane was composed of an inner dense sub-nanoporous separating layer and an outer mesoporous, thinner, and hydrophilic degrading layer. Consequently, the activated PNF-2 membrane module was capable of simultaneously removing more than 99% of trimethoprim and sulfamethoxazole, almost without sacrificing water permeability (21.1 L m−2 h−1 bar−1), indicating that the hydrophilic mesoporous degradation layer brings little additional hydraulic resistance. The removal rates of both selected antibiotics in practical water environment were slightly higher than those in the simulated water environment, mostly because of the increased steric hindrance of contaminants in the former case. Besides, after long-term operation up to 76 h, the minimum total and reversible fouling ratios (7.97% and 2.43%) together with only sporadic foulants remaining on the physically rinsed membrane surface were obtained. This is bound to greatly improve its cleaning threshold and maintain superior service performance. Moreover, the acute and chronic toxicities of its permeate were dramatically cut down to 4.14% and 1.56%, respectively. All these strongly prove that the dual functions of the PNF-2 membrane are synergetic in an uninterrupted permeating process and will boost the efficient removal of trace organic contaminants in real applications.
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JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2023
·
IF 3.6
Shaodong Hu,Quan Zhao,Yapeng Niu,Canxuan Luo,Zhixuan Zhang,Bin He,Hui Hao
Biodegradable polymer materials are attractive in various biomedical applications. Herein, we report a block aliphatic copolymer, polycaprolactone-block-polyglycolide (PCL-b-PGA, denoted as PCLGA), and explored the polymer composition effect on the thermal and degradation behaviors. Three copolymers with different PCL/PGA weight ratios were obtained by a two-step ring opening polymerization. The chemical structures and compositions of copolymers were studied by NMR and FT-IR. The thermal behaviors were investigated by DSC and TGA. The in vitro degradation in phosphate buffer saline and in vivo degradation as an implant in rats were evaluated and the hygroscopic rate and polymer weight loss changes were monitored in an eight-week period. PCLGA with a higher PCL composition showed a slower degradation in vitro and in vivo. These PCLGAs with different degradation rates could be used as biomedical implants for potential application in drug delivery and tissue repair.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 4
·3464
·
IF 5.6
Shan-Cheng Yi,Yu-Hang Wu,Rui-Nan Yang,Dong-Zhen Li,Hazem Abdelnabby,Man-Qun Wang
Natural enemies such as parasitoids and parasites depend on sensitive olfactory to search for their specific hosts. Herbivore-induced plant volatiles (HIPVs) are vital components in providing host information for many natural enemies of herbivores. However, the olfactory-related proteins involved in the recognition of HIPVs are rarely reported. In this study, we established an exhaustive tissue and developmental expression profile of odorant-binding proteins (OBPs) fromDastarcus helophoroides, an essential natural enemy in the forestry ecosystem. Twenty DhelOBPs displayed various expression patterns in different organs and adult physiological states, suggesting a potential involvement in olfactory perception. In silico AlphaFold2-based modeling and molecular docking showed similar binding energies between six DhelOBPs (DhelOBP4, 5, 6, 14, 18, and 20) and HIPVs fromPinus massoniana. While in vitro fluorescence competitive binding assays showed only recombinant DhelOBP4, the most highly expressed in the antennae of emerging adults could bind to HIPVs with high binding affinities. RNAi-mediated behavioral assays indicated that DhelOBP4 was an essential functional protein forD. helophoroidesadults recognizing two behaviorally attractive substances:p-cymene and γ-terpinene. Further binding conformation analyses revealed that Phe 54, Val 56, and Phe 71 might be the key binding sites for DhelOBP4 interacting with HIPVs. In conclusion, our results provide an essential molecular basis for the olfactory perception ofD. helophoroidesand reliable evidence for recognizing the HIPVs of natural enemies from insect OBPs’ perspective.Keywords:herbivore-induced plant volatiles;odorant-binding proteins;Dastarcus helophoroides;binding affinities;behaviorally attractive substances
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危险品化学品经营许可证（带存储）