MICROCHIMICA ACTA
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2024
·Vol. 191
·期 12
·1-10
·
IF 5.3
Ye Tai,Luo Zheng,Che Yueyue,Yuan Min,Cao Hui,Hao Liling,Zhang Qian,Xie Yongxin,Zhang Kaisen,Xu Fei
An inverted DNA tetrahedron–mediated modular DNA walker was developed for the determination of sulfadimethoxine. The inverted DNA tetrahedron scaffold raises several advantages of recognition module including appropriate lateral space, multiple recognition domains, and cost-effectiveness. The proposed inverted DNA tetrahedron-based recognition module exhibited better binding affinity and kinetics toward target antibiotic than that of other DNA tetrahedron counterparts. Upon specific binding with target, the released bipedal DNA walking strand hops to the signal amplification module and moves stochastically with assistant of nicking enzyme. By coupling these two modules, a good linear relationship between the fluorescence intensity of supernatant and the concentration of sulfadimethoxine was achieved in the range 0.1–100 nM, and the limit of detection was 64.7 pM. Furthermore, this modular DNA walker had also successfully applied to spiked honey and milk samples with satisfactory recoveries from 91.5 to 108.8%, demonstrating its practical sensing capability. Graphical
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Waste and Biomass Valorization
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2025
·
IF 2.8
Zhou Caixue,Dai Shuxia,Yin Li,Zhuo Yu,Zhao Nannan,Li Dehai,Sun Changyan
Purpose In this paper, we use Pinus pumila (Pall.) Pinecone to extract essential oil and improve its utilization. Methods The study used hydrodistillation to extract essential oil from Pinus pumila (Pall.) pinecone. Inhibition analysis of E. coli using extracted essential oils was evaluated to reveal the inhibitory activity by assessing growth curves, conductivity, nucleic acids, proteins, enzyme activities, and protein synthesis. Results The primary constituents, as shown by GC-MS chemical composition study, were α-pinitol, β-pinene and limonene. The results of the bacteriostatic activity indicated that the inhibitory zone (DIZ) had a diameter of 14.93 ± 0.14 mm, the minimum bactericidal concentration (MBC) was 16.0 µL/mL, and the minimum inhibitory concentration (MIC) was 8.0 µL/mL. Sweeping electron microscopy and laser confocal also demonstrated the disruption of cell wall and cellular enzymes, analyzed by CATase and ATPase activities, which showed a decrease in enzyme activity, and SDS-PAGE analysis showed its ability to inhibit protein synthesis, which leads to cellular damage. Conclusion The present study emphasizes the sustainable use of Pinus pumila (Pall.) Pinecone for economic opening of food preservation in addition to inhibition of spoilage bacteria.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-12
·
IF 15.7
Wang Dun,Hu Sanlue,Li Titi,Chang Caiyun,Li Senlin,Guo Songde,Li Huan,Liu Qingming,Gong Jinqiu,Zhou Jun,Han Cuiping
Aqueous zinc ion batteries exhibit great prospects due to their low cost and high safety, while their lifespan is limited by severe dendritic growth problems. Herein, we develop an anti-dendrite hot-pressing separator interlayer through a mass-producible hot-pressing strategy, by spreading metal-organic framework (MOF) precursor on nonwoven matrix followed by a simple hot-pressing process. The in situ modification of MOF crystals on fiber surface processes abundant nitrogenous functional groups and high specific surface area (190.8 m 2 g −1 ) with a strong attraction to Zn 2+ . These features contribute to a staged deposition behavior to promote uniform nucleation at high concentrations and two-dimensional grain growth at low concentrations. Consequently, Zn | |Zn symmetrical cells with hot-pressing separator interlayer demonstrate cycle lives of 3000 hours at 2 mA cm −2 , 2 mAh cm −2 . Moreover, Zn | |I 2 pouch batteries with hot-pressing separator interlayer realizes 840 cycles lifespan with a capacity retention of 90.9% and a final discharge capacity of 110.6 mAh at 25 °C.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-13
·
IF 14.7
Liu Qinglu,Tang Tang,Tian Ziyu,Ding Shiwen,Wang Linqin,Chen Dexin,Wang Zhiwei,Zheng Wentao,Lee Husileng,Lu Xingyu,Miao Xiaohe,Liu Lin,Sun Licheng
Ion-solvating membranes have been gaining increasing attention as core components of electrochemical energy conversion and storage devices. However, the development of ion-solvating membranes with low ion resistance and high ion selectivity still poses challenges. In order to propose an effective strategy for high-performance ion-solvating membranes, this study conducted a comprehensive investigation on watermelon skin membranes through a combination of experimental research and molecular dynamics simulation. The micropores and continuous hydrogen-bonding networks constructed by the synergistic effect of cellulose fiber and pectin enable the hypodermis of watermelon skin membranes to have a high ion conductivity of 282.3 mS cm −1 (room temperature, saturated with 1 M KOH). The negatively charged groups and hydroxyl groups on the microporous channels increase the formate penetration resistance of watermelon skin membranes in contrast to commercially available membranes, and this is crucial for CO 2 electroreduction. Therefore, the confinement of proton donors and negatively charged groups within three-dimensional microporous polymers gives inspiration for the design of high-performance ion-solvating membranes.
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EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2024
·
IF 3
Kong Xinxin,Zhang Lingli,Cai Ling,Cheng Shengyu,Li Xi-Ling,Jin Toufeng,Min Jun Zhe
Selenium (Se) is a vital trace element for mammals, but excessive intake can lead to selenium poisoning. To manage Se intake properly, this study developed a novel method using ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). This technique allows the simultaneous detection of three selenoamino acids in supplements. The three selenoamino acids were fully separated within 3 min. The method demonstrated excellent linearity (R 2 ≥ 0.9993), high sensitivity (detection limits were 2.49–2.92 fmol), and good precision (intraday and interday precision ranged from 2.87 to 13.54%). This study applied this method to analyze the content of selenoamino acids in organic selenium supplements from various sources and to dynamically monitor their absorption and excretion in the body after consumption. The novel approach offers a new strategy for the quality control of selenium-containing supplements and for studying selenium absorption and metabolism. Graphical abstract
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Friction
·
2024
·Vol. 12
·期 11
·2480-2490
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IF 6.3
Hou Yacong,Chen Lei,Zhang Zheng,Chang Jinlin,Weng Ding,Ma Yuan,Wang Jiadao
Low friction ice sliding interfaces were critical for ski performance optimization. Traditional fluorinated ski waxes have attracted considerable attention for enhancing the hydrophobicity, anti-wearing, and oxidation resistance of ski-ice base. However, the toxicity and complexity of the waxing process limited batch manufacturing of low-cost and non-toxic ski-ice base, what is more, the wax covering on the base wore and failed during skiing due to the friction between ski and ice. Herein, we demonstrated a novel ultra-high molecular weight polyethylene (UHMWPE) composite that could maintain a low coefficient of friction (COF) with about 0.026 for at least 160 min when skiing on the ice. Microcapsule (MS) could release liquid (liquid paraffin (LP)). The released LP further enhanced the hydrophobicity of UHMWPE’s surfaces when friction occurred, which would maintain the stability and durability of the water film, and achieved superior and long-lasting friction resistance. Compared with other microcapsules with lower hydrophobic core, microcapsules with LP performed the best in reducing the friction of ski base from 0.126 to 0.024. Meanwhile, the COF of the surface kept at about 0.02 even after 12 rapid temperature changes. The presented UHMWPE composite of encapsulated liquids showed great potential and broad application owing to its simplicity and efficiency in winter sports.
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CHROMATOGRAPHIA
·
2012
·Vol. 75
·期 9
·499-511
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IF 1.195
Zhang Chunhua,Wu Huiqin,Huang Xiaolan,Zhu Zhixin,Luo Huitai,Huang Fang,Lin Xiaoshan
A sensitive and selective method for simultaneous determination of 29 toxic alkaloids in human blood and 31 in urine using high-performance liquid chromatography–electrospray ionization-tandem mass spectrometry was developed and validated. The samples were diluted with 0.1 mol L −1 HCl, and the target alkaloids were purified by solid phase extraction. The separation of 31 alkaloids was carried out on a C18 column using a gradient mobile phase with 10 mmol L −1 ammonium formate in water with 0.1% formic acid and methanol at the rate of 0.25 mL min −1 . The triple-quadrupole mass spectrometer equipped with an electrospray source in the positive mode was set up in the dynamic multiple reactions monitoring mode (dynamic MRM) to detect the ion transitions of 31 alkaloids. The calibration curves were linear over a range of 0.5–400, 1–400, or 4–400 μg L −1 for target alkaloids in human blood and urine, and the correlation coefficients ( r
2 ) was higher than 0.9943. The limit of determination and limit of quantification were 0.2–1 and 0.5–4 μg L −1 for blood and urine, respectively. The only exceptions were sanguinarine and chelerythrine in human blood. All the target alkaloids were stable under the test condition. In addition, the solvent effect and reconstituted solution were investigated. The method was validated and proved to be accurate and precise over the studied concentrations and suitable for poisoning diagnosis and forensic toxicology.
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WATER AIR AND SOIL POLLUTION
·
2012
·Vol. 223
·期 6
·3461-3469
·
IF 1.625
Yang Huijuan,Zhang Qianli,Zhao Jinjin,Tu Yifeng
The separation on microchip provides the advantages including high efficiency, increased throughput, reduced quantities of hazardous materials, cost saving, relatively facile instrumentation, improved portability, etc. A technique of micellar electrokinetic chromatography (MEKC) coupled with amperometric detection has been actualized on a polydimethylsiloxane microchip for the rapid separation and determination of three phenolic xenoestrogens as octylphenol (OP), 4-nonylphenol (4-NP), and bisphenol A (BPA). The baseline separation of these phenolic xenoestrogens is successfully obtained within 55 s under the optimized MEKC conditions with borate running buffer of pH 8.0 containing sodium dodecyl sulfate and β-cyclodextrin. The linear range for OP, 4-NP, and BPA are 20–1,000, 15–1,000, and 20–1,000 μg/L with the detection limit of 5.0, 4.0, and 3.0 μg/L, respectively. The present method is successfully applied for the determination of these phenolic xenoestrogens in some industrial wastewater samples from mainland of China with the recoveries ranged from 90.2 to 109.4 %.
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CHROMATOGRAPHIA
·
2012
·Vol. 75
·期 5
·289-296
·
IF 1.195
Zhang Yulin,Huang Liang,Chen Qinhua,Chen Zilin
A silica monolithic column chemically modified with l -pipecolic acid as chiral stationary phase has been developed for chiral separation of dansyl amino acids by capillary electrochromatography–mass spectrometry (CEC–MS). The monolithic column was prepared by a sol–gel process and subsequent chemical modification by l -pipecolic acid as chiral selector with 3-glycidoxypropyltrimethoxysilane as spacer. Interestingly, it was found that the l -pipecolic acid-modified monolithic column can hold copper(II) ions tightly after loading Cu(II) ions during column preparation and conditioning and allows CEC separation to be conducted based on chiral ligand exchange with MS detection by a mobile phase without copper ions. It has been demonstrated that the chiral monolithic column operates well for enantioseparation of several dansyl amino acids by CEC–MS.
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Nano Research
·
2024
·
IF 9.5
Liu Fang,Zou Lingyun,Chen Yongcheng,Liu Zuolong,Huang Yue,Jin Qiao,Ji Jian
Biofilm-associated bacterial infection brings serious threats to global public health owing to serious antibiotic resistance. It is urgently needed to develop innovative strategies to combat biofilm-associated bacterial infections. Polymyxins stand out as the last line of defense against Gram-negative bacteria. However, serious nephrotoxicity of polymyxins severely limits their clinical utility. Herein, a hypoxia-responsive liposome is designed as the nanocarrier of polymyxin B (PMB) to combat biofilms developed by Gram-negative bacteria. A metronidazole modified lipid (hypoxia-responsive lipid (HRLipid)) is synthesized to fabricate hypoxia-responsive liposomes (HRLip). PMB loaded hypoxia-responsive liposomes (HRL-PMB) is then prepared to mitigate the nephrotoxicity of PMB while preserving its excellent bactericidal activity. HRL-PMB shows very low hemolysis and cytotoxicity due to liposomal encapsulation of PMB. PMB can be readily released from HRL-PMB in response to hypoxic biofilm microenvironment, exerting its bactericidal activity to realize biofilm eradication. The excellent in vivo antibiofilm ability of HRL-PMB is confirmed by a Pseudomonas aeruginosa infected zebrafish model and a P. aeruginosa pneumonia infection model. Meanwhile, HRL-PMB can greatly reduce the nephrotoxicity of PMB after intravenous injection. The hypoxia-sensitive liposomes held great promise to improve the biosafety of highly toxic antibiotics while preserving their intrinsic bactericidal ability, which may provide an innovative strategy for combating biofilm-associated infections.
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Nano Research
·
2024
·
IF 9.5
Hong Jiawei,Xian Shiyun,Zheng Shusen,Wang Hangxiang,Jiang Donghai
Resistance to gemcitabine in pancreatic cancer poses a significant clinical challenge. Further investigation is warranted to assess whether nano-formulation strategy can be employed to enhance the sensitivity of resistant strains to gemcitabine therapy. In this study, using gemcitabine-resistant pancreatic cancer cell lines, we examined the therapeutic potential of a gemcitabine nanodelivery platform and assessed the ability to overcome drug resistance against resistant strains. Silencing of human equilibrative nucleoside transporter 1 (hENT1) led to reduced cellular uptake of gemcitabine, resulting in chemoresistance in pancreatic cancer. Gemcitabine nanoparticles circumvented the entry blockade caused by hENT1 silencing through endocytosis. Nanoparticle entry via clathrin-mediated endocytosis increased intracellular gemcitabine accumulation in gemcitabine-resistant pancreatic cancer cells. Moreover, gemcitabine nanoparticles are preferential in vivo delivery to tumor tissues, likely due to the enhanced permeability and retention effect. In comparison to free gemcitabine, gemcitabine nanoparticles demonstrate a more pronounced cytotoxic effect on gemcitabine-resistant pancreatic cancer cells, with favorable biosafety. This study improved the efficacy of gemcitabine through nanotechnology, providing a novel strategy to address gemcitabine-resistant pancreatic cancer.
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Particle and Fibre Toxicology
·
2024
·Vol. 21
·期 1
·1-19
·
IF 7.2
Fan Junjie,Liu Li,Lu Yongling,Chen Qian,Fan Shijun,Yang Yongjun,Long Yupeng,Liu Xin
Background The global use of plastic materials has undergone rapid expansion, resulting in the substantial generation of degraded and synthetic microplastics and nanoplastics (MNPs), which have the potential to impose significant environmental burdens and cause harmful effects on living organisms. Despite this, the detrimental impacts of MNPs exposure towards host cells and tissues have not been thoroughly characterized. Results In the present study, we have elucidated a previously unidentified hepatotoxic effect of 20 nm synthetic polystyrene nanoparticles (PSNPs), rather than larger PS beads, by selectively inducing necroptosis in macrophages. Mechanistically, 20 nm PSNPs were rapidly internalized by macrophages and accumulated in the mitochondria, where they disrupted mitochondrial integrity, leading to heightened production of mitochondrial reactive oxygen species (mtROS). This elevated mtROS generation essentially triggered necroptosis in macrophages, resulting in enhanced crosstalk with hepatocytes, ultimately leading to hepatocyte damage. Additionally, it was demonstrated that PSNPs induced necroptosis and promoted acute liver injury in mice. This harmful effect was significantly mitigated by the administration of a necroptosis inhibitor or systemic depletion of macrophages prior to PSNPs injection. Conclusion Collectively, our study suggests a profound toxicity of environmental PSNP exposure by triggering macrophage necroptosis, which in turn induces hepatotoxicity via intercellular crosstalk between macrophages and hepatocytes in the hepatic microenvironment.
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