FOOD CHEMISTRY
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2022
·
IF 9.231
Jinmiao Sun,Yixin Dong,Xun Li,Fei Wang,Yu Zhang
Phytoferritin was explored as an attractive nanocarrier to encapsulate bioactive compounds due to its excellent stability and biocompatibility. In the present study, a novel phytoferritin derived from alfalfa (Medicago sativa ) was successfully expressed, purified and characterized. Results confirmed that alfalfa ferritin, self-assembled by 24 subunits, formed a spherical hollow structure. Baicalein exhibits superior antioxidant properties and nutritious values, but low bioavailability and solubility limit its application. Herein, we fabricated water-soluble chitosan-ferritin-baicalein nanoparticles to overcome its drawbacks. It was calculated that one apoferritin cage could encapsulate 52 molecules of baicalein. Moreover, chitosan-ferritin-baicalein nanoparticles prolonged the release of baicalein in simulated gastrointestinal tract digestion. Caco-2 cell monolayer absorption analysis demonstrated that baicalein encapsulated within ferritin-chitosan double layers was more efficient in cellular transportation. These results indicated that alfalfa ferritin, as a novel cage-like protein, has potential application in improving the bioavailability of insoluble bioactive molecules.
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Huanhuan Cui,Cuie Tang,Shan Wu,David Julian McClements,Shilin Liu,Bin Li,Yan Li
In this study, chitosan-based bigels were fabricated, where glycerol monolaurate was added in MCT oil to produce a gelled lipid phase and cinnamaldehyde was included in the lipid phase in order to act as a crosslinking agent. The synergistic effect of pH on chemical crosslinking effects was investigated. The potential of using these bigels as an alternative to cream was also investigated. The pH of the aqueous phase played an important role in controlling the extent of the Schiff-base reaction promoted by cinnamaldehyde. At pH 3.8, the bigels formed were homogenous but at pH 5.0 and 5.5 they exhibited phase separation, which highlighted the importance of chemical crosslinking. To better mimic the properties of real cream, span 80 was added to create a more homogeneous and smoother structure of the bigels. These bigels might provide a healthy and more sustainable alterative to food products that contain plastic fats, like cream.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
·
IF 5.518
Xin-Hao Yu,Fu-Zhen Zhou,Yong-Kang Xi,Xiao-Nan Huang,Shou-Wei Yin,Xiao-Quan Yang
Water-in-oil (W/O) emulsions have numerous applications in many industries like foods, pharmaceuticals, in particular biphasic bio-catalysis. However, most of them are stabilized by surfactants or synthetic particles, which lead to limited uses in foods. Here, a W/O type Pickering emulsion was successfully prepared firstly by ordered ethyl cellulose-chitosan complex particles (ECCPs) and used for a recyclable biocatalytic micro-reactor. The performances of emulsions with different ethyl cellulose-chitosan ratios, water phase fraction and particle concentration were discussed. The type of emulsions could be shifted from W/O to W/O/W and O/W as water fraction increased. Interestingly, a recyclable bio-catalytic microreactor platform was constructed using such novel W/O emulsions, and the activity of lipase was significantly increased by encapsulating it into Pickering emulsions, and was 6.3-times higher than that of free lipase in biphasic system. In particular, the encapsulated lipase remained stable and could be recycled over 15 times without the loss of activity.
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Marine Drugs
·
2022
·Vol. 20
·期 2
·86
·
IF 6.085
Jingmin Cui,Xia Ji,Yingqi Mi,Qin Miao,Fang Dong,Wenqiang Tan,Zhanyong Guo
N-2-hydroxypropyltrimethyl ammonium chloride chitosan (HACC), a cationic quaternary ammonium salt polymer exhibiting good solubility in water, is widely used because of its low toxicity and good biocompatibility. Herein, through ion exchange reaction, we preparedN-2-hydroxypropyltrimethyl ammonium chitosan derivatives bearing amino acid Schiff bases with good biological activities. The accuracy of the structures was verified by FT-IR and1H NMR. The antibacterial activity, antifungal activity, and scavenging ability of DPPH radical and superoxide radical of HACC derivatives were significantly improved compared with that of HACC. In particular, HACGM (HACC-potassium 2-((2-hydroxy-3-methoxybenzylidene)amino)acetate) and HACGB (HACC-potassium 2-((5-bromo-2-hydroxybenzylidene)amino)acetate) showed good inhibitory effect on bacteria and fungi, includingStaphylococcus aureus,Escherichia coli,Botrytis cinerea,andFusarium oxysporumf. sp.cubense. The inhibition rate of HACGB onStaphylococcus aureusandEscherichia colicould reach 100% at the concentration of 0.1 mg/mL, and the inhibition rate of HACGM and HACGB onBotrytis cinereaandFusarium oxysporumf. sp.cubensecould also reach 100% at the concentration of 0.5 mg/mL. Improving antimicrobial and antioxidant activities of HACC could provide ideas and experiences for the development and utilization of chitosan derivatives.Keywords:N-2-hydroxypropyltrimethyl ammonium chloride chitosan;amino acid Schiff bases;antimicrobial activity;antioxidant activity
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Chaoyi Shen,Menglu Wu,Cui Sun,Jiangkuo Li,Di Wu,Chongde Sun,Yong He,Kunsong Chen
In this work, the solution blow spinning (SBS) technique was used to rapidly fabricate the thymol (THY)/2-hydroxypropyl-β-cyclodextrin (HPβCD) inclusion complexes loaded chitosan (CS)/polycaprolactone (PCL) nanofibrous films for fruit preservation and packaging. XRD results indicated that the THY/HPβCD inclusion complexes were successfully incorporated into the CS/PCL nanofibers . The nanofibrous films had an increase of average diameters of nanofibers from 243.84 nm to 560.55 nm, an enhancement of water vapor permeability, a decrease of the crystallinity , and a hydrophilic surface after the incorporation. FTIR and thermal analysis showed that the thermal stability was also improved due to the formation of hydrogen bonds between THY/HPβCD inclusion complexes and CS/PCL nanofibers. The developed films obtained a long-term continuous release of THY during 240 h, and had a good antifungal activity in vitro and in vivo . The above results indicated the promising prospects of SBS in developing antifungal nanofibrous films for postharvest fruit.
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CELLULOSE
·
2022
·Vol. 29
·期 4
·2731-2742
·
IF 6.123
Liu Zihan,Luo Yapei,Zhao Xiaomin,Zheng Keying,Wu Minghua,Wang Lili
Natural antibacterial agents have attracted increasing attention due to safety, ecological, and environmental protection concerns. In this study, hinokitiol-grafted-chitosan (HTCS) was prepared via the Mannich reaction and used as an antibacterial agent for the treatment of cotton fabric. The results showed that, compared with chitosan (CS) and hinokitiol (HT) alone, HTCS exhibited a lower value of minimum inhibitory concentration (MIC). Compared with the fabrics treated by CS and HT, the antibacterial rate of the treated fabric against Escherichia coli using HTCS as antibacterial agent increased by approximately 90% and 27%, the bacterial reduction rate against Staphylococcus aureus increased by about 58% and 39%. The HTCS-treated cotton fabric possessed good antibacterial properties even after 25 washing cycles. Moreover, the antibacterial cotton fabric retained original hydrophilicity, handle, and strength. Consequently, HTCS has great potential as a natural antibacterial agent for textiles.
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ACS Applied Polymer Materials
·
2022
·
IF 4.855
Lu Yang,Yunhong Cong,Jingyi Zhang,Zheyuan Gu,Lili Shen,Huailing Gao,Xianyu Zheng,Mohan Wang,Jiacai He
Uniform and porous chitosan-based microparticles with injectable and shape-memory properties are particularly attractive due to their promising application potential for tissue engineering and regenerative medicine. However, simple and efficient methods for producing this kind of microparticle are still desirable. In this study, we report that uniform, injectable, and shape-memory chitosan microsponges were efficiently prepared by combining microfluidic emulsion with further freezing and in situ thawing processes without using any potentially cytotoxic chemical cross-linker. The produced chitosan microsponges have controllable size and could be easily injected with syringe needles. Structural observations confirmed that the chitosan microsponge had an interconnected porous structure with pore size of several micrometers and could withstand a large compressive strain of ∼93% and then recover ∼96% of its initial diameter without structural damage. The chitosan microsponges showed a high porosity (∼84%) and swelling ratio (∼3800%) as well as good antibacterial activity. Additionally, an in vitro cell coculturing investigation revealed that they also had good biocompatibility and exhibited great superiority to support cell adhesion and proliferation in three dimensions. The kind of chitosan microsponge presented here has great potential to serve as cell carriers for biomedical applications, especially as injectable scaffolds for regeneration and reconstruction of tissue defects.
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ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Yingqi Chen,Weibei Sheng,Jianjing Lin,Chongzhou Fang,Jiapeng Deng,Peng Zhang,Meng Zhou,Peng Liu,Jian Weng,Fei Yu,Deli Wang,Bin Kang,Hui Zeng
Natural polysaccharide (NPH)-based injectable hydrogels have shown great potential for critical-sized bone defect repair. However, their osteogenic, angiogenic, and mechanical properties are insufficient. Here, MgO nanoparticles (NPs) were incorporated into a newly synthesized water-soluble phosphocreatine-functionalized chitosan (CSMP) water solution to form an injectable hydrogel (CSMP-MgO) via supramolecular combination between phosphate groups in CSMP and magnesium in MgO NPs to circumvent these drawbacks of chitosan-based injectable hydrogels. Water-soluble chitosan deviate CSMP was first synthesized by grafting methacrylic anhydride and phosphocreatine into a chitosan chain in a one-step lyophilization process. The phosphocreatine in this hydrogel not only provides sites to combine with MgO NPs to form supramolecular binding but also serves as the reservoir to control Mg2+ release. As a result, the lyophilized CSMP-MgO hydrogels presented a porous structure with some small holes in the pore wall, and the pore diameters ranged from 50 to 100 μm. The CSMP-MgO injectable hydrogels were restricted from swelling in DI water (lowest swelling ratio was 16.0 ± 1.1 g/g) and presented no brittle failure during compression even at a strain above 85% (maximum compressive strength was 195.0 kPa) versus the control groups (28.0 and 41.3 kPa for CSMP and CSMP-MgO (0.5) hydrogels), with regulated Mg2+ release in a stable and sustained manner. The CSMP-MgO injectable hydrogels promoted in vitro calcium phosphate (hydroxyapatite (HA) and tetracalcium phosphate (TTCP)) deposition in supersaturated calcium phosphate solution and presented no cytotoxicity to MC3T3-E1 cells; the CSMP-MgO hydrogel promoted MC3T3-E1 cell osteogenic differentiation with upregulation of BSP, OPN, and Osterix osteogenic gene expression and mineralization and HUVEC tube formation. Among them, CSMP-MgO (5) presented most of these properties. Moreover, this hydrogel (CSMP-MgO (5)) showed an excellent ability to promote new bone formation in critical-sized calvarial defects in rats. Thus, the CSMP-MgO injectable hydrogel shows great promise for bone regeneration.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Lirong Yang,Xuan Luo,Lin Yan,Yawen Zhou,Shaoqing Yu,Hui Ju,Yan Wang,Lin Zhang
In the present study, a novel eco-friendly foam (CS-AMP) was successfully prepared by grafting adenosine 5′-monophosphate (AMP) with high biocompatibility onto environment-friendly monomer chitosan (CS). The CS-AMP foam was used to absorb uranium and exhibited an efficient selective adsorption of uranium from aqueous solution and natural seawater . Due to the phosphate group of AMP, the CS-AMP foam shows an excellent adsorption capacity of 311 mg/g (pH 5.0 and 308 K) for uranium, which is much higher than that of CS-PTA foam without modification by AMP. The adsorption process is fitted well with Sips isothermal and pseudo-second-order kinetic models . As a highly selective uranium adsorbent, it is expected to be applied to extract uranium from wastewater and seawater.
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RSC Advances
·
2022
·Vol. 12
·期 5
·3147-3156
·
IF 4.036
Bingbing Yu,Yao Chen,Lijun Zhu,Mengmeng Ban,Li Yang,Yeda Zeng,Shijie Li,Chunzhi Tang,Danyan Zhang,Xiaoqing Chen
The purpose of this research was to develop an efficient and non-destructive method for decolorizing of polysaccharides extracted from Isaria cicadae Miquel by magnetic chitosan microspheres (MCM). The optimum decolorization parameters were achieved by response surface methodology as follows: the MCM amount was 8.0%, the adsorption temperature was 48 °C, the adsorption time was 82 min and the pH was 7. Under these optimal conditions, the Dr%, Rr%, and Kc were 90.31 ± 0.12%, 95.40 ± 0.11% and 19.66 ± 0.49, respectively. MCM adsorption of pigment molecules was a spontaneous and endothermic process that could be fitted with the pseudo-second-order equation and the Freundlich equation. Besides, the adsorption mechanism could be controlled by multiple-diffusion steps, including film diffusion and intra-particle diffusion. Furthermore, MCM is a recyclable material. Adsorption with MCM is a promising method to remove pigment molecules of polysaccharide, it may replace the traditional decolorization method.
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Polymers
·
2023
·Vol. 15
·期 10
·2352
·
IF 5
Linxinzheng Guo,Kun Hu,Haibo Wang
Polyvinyl alcohol (PVA) is a polymeric material with good biocompatibility, excellent hydrophilicity, and a large number of hydroxyl groups. However, due to its insufficient mechanical properties and poor inhibition of bacteria, it has a lack of applications in wound dressings, stent materials, and other fields. In this study, a simple method was used to prepare composite gel materials: Ag@MXene-HACC-PVA hydrogels with a double-network structure were prepared using an acetal reaction. Due to the double cross-linked interaction, the hydrogel has good mechanical properties and is resistant to swelling. The adhesion and bacterial inhibition were enhanced due to the addition of HACC. In addition, the strain sensing properties of this conductive hydrogel were stable, and the GF (specification factor) was 1.7617 at 40–90% strain. Therefore, the dual-network hydrogel with excellent sensing properties, adhesion properties, antibacterial properties, and cytocompatibility has potential applications in biomedical materials, especially as a tissue engineering repair material.
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EUROPEAN POLYMER JOURNAL
·
2023
·
IF 6
Jianhua Lv,Meihua Jiang,Yaru Fang,Wenchang Zhang,Mi Wu,Fanghao Zheng,Kaijun Lei,Lei Shang,Yan Zhao
The biomimetic chitosan derivatives synthesized by chemical coupling of L-arginine onto chitosan with low molecular weight, named Arg-CS, which was prepared by mimicking the arginine-rich peptides, to achieve the permeability performance of Cell-penetrating peptides (CPPs). The physicochemical properties of the Arg-CSs were characterized via Attenuated total reflection Fourier transform infrared (ATR-FTIR) and 1 H-nuclear magnetic resonance spectroscopy ( 1 H NMR), and high-performance liquid chromatography (HPLC). The thermal stability of Arg-CS was improved by approximately 80 °C, compared to the pure chitosan. The maximum cumulative amounts of aspirin for Arg-CS 4.7 k, Arg-CS 5.2 k, and Arg-CS 6.3 k were 32.85 µg/cm 2 , 22.98 µg/cm 2 , and 17.51 µg/cm 2 at 8 h, respectively, presenting a phenomenon of increasing of aspirin permeation with decreasing molecular weight of Arg-CS. Arg-CS with low molecular weight was more conducive to improve the penetration of aspirin by changing the secondary structure of keratin from β-sheets to random coil. The molecular docking also suggested that Arg-CS had stronger affinity to keratin by strong hydrogen bond interactions. These results indicated that the nonirritant and thermally stable Arg-CS with lower molecular weight can be used to enhance the permeation of aspirin and applied in the field of transdermal drug delivery.
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