JOURNAL OF CONTROLLED RELEASE
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2026
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IF 11.5
Ying-Tong Ye,Xi Xiao,Hong-Ying Xia,Jie Li,Zhen-Zhen Gong,Ai-Zheng Chen,Shi-Bin Wang,Ranjith Kumar Kankala
Despite the success of various intelligent materials in meeting the multiple needs of diabetic ulcer wounds (DUW) healing, it is often challenging to consider the entire healing process, from early antibacterial and anti-inflammatory effects to late matrix remodeling and angiogenesis characteristics, by releasing multiple active ingredients in stages and on demand. Herein, we designed a versatile bilayer (GCM/Clean-GDA/Cure) hydrogel dressing with light-controlled, spatiotemporal sequential release of MXene nanosheets (MX NSs)/nitric oxide (NO) gas for the healing of methicillin-resistant Staphylococcus aureus (MRSA) biofilm-infected DUWs. Initially, the lower GCM layer comprised carboxymethyl chitosan-gelatin and cerium oxide (CeO 2 )-decorated MX NSs (CO-MX), and the upper GDA layer consisted of dopamine-modified methacrylated gelatin (GelMA-DA) with L-arginine (L-Arg) as a precursor. Under NIR irradiation, the GCM hydrogel releases photothermal-induced CO-MX NSs as self-regenerating antioxidants, exhibiting 98.25 % inhibition against MRSA and 96.37 % inhibition against Pneumonia aeruginosa. The abundant oxygen vacancies and reversible Ce(III)/Ce(IV) redox pairs of the released Ce species from CO-MX NSs could attenuate inflammation and promote early macrophage polarization. The light-assisted gel–sol thermal transition of the lower GCM layer further exposes the upper GDA layer, providing a conducive microenvironment for angiogenesis and tissue remodeling. L-Arg in the GDA layer could enable light-controlled in situ release of NO gas activated by elevated reactive oxygen species (ROS), reaching 7.71 μM within 10 min of exposure to NIR light. Thus, the NIR-assisted bilayer treatment group achieved a 97.74 % wound closure rate. Finally, the transcriptomic analysis validated the activation of regenerative pathways and suppression of excessive inflammatory and metabolic stress signals. Together, the photo-controlled sequential release of MX NSs and NO gas from the bilayer could expand the application of light-responsive nanomaterials, providing an innovative therapeutic modality for in situ treatment of diabetic wounds infected by highly drug-resistant MRSA biofilms.
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Nature Communications
·
2026
·Vol. 17
·期 1
·173
·
IF 15.7
Luo Ruifeng,Liu Jinwei,Dai Linxin,Duan Tianshun,Zhang Qingwen,Gao Cheng,Wang Ruibing
Inflammatory bowel disease (IBD) involves elevated intestinal reactive oxygen species (ROS) and microbial imbalance. A key challenge is that current delivery systems cannot adequately protect active agents, such as antioxidants and probiotics, through the harsh gastric environment nor precisely deliver them to inflamed intestinal sites. Here, we present the EcN@PDA@HP biohybrid robot, composed of stress-trained microalgae, probiotics, and an intestinal braking system, enabling rapid gastric passage and precise retention in inflamed regions. Stress-trained Haematococcus pluvialis (HP) developed a thickened cell wall for gastric resilience, maintained flagella-driven motility, and enhanced astaxanthin (AST) production. Polydopamine (PDA)-coated Escherichia coli Nissle 1917 (EcN) anchors onto HP via host-guest interactions. In vivo, PDA preserves EcN activity and enables specific adhesion to inflamed sites. By combining antioxidizing AST and bacteriotherapy, EcN@PDA@HP effectively alleviates male murine IBD models via ROS scavenging and microbiota restoration, offering a promising strategy for diverse IBD conditions.
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ANALYST
·
2026
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IF 3.3
Xueying Zhu,Shuo Tian,Shuyun Chen,Yunsen Wang,Dianping Tang
As a key alkaloid, the concentration of xanthine in the body often reflects various physiological or pathological states of the human body. However, conventional methods for xanthine detection often suffer from time-consuming procedures and a narrow linear range. The present study screened 30 nanozymes with varying Fe/S molar ratios using a hydrogen peroxide-3,3',5,5'-tetramethylbenzidine (H2O2-TMB) colorimetric system and identified FeS-loaded nitrogen-doped carbon nanozyme (Fe300S5-NC) as a highly efficient catalyst. Inspired by the biological metabolic pathway of xanthine, an XOD/Fe300S5-NC/TMB colorimetric system was constructed for the detection of xanthine. The constructed colorimetric sensing platform demonstrated facile operation and rapid response, with a linear xanthine detection range of 10–1000 μM and a detection limit of 0.97 μM. This work provides a viable strategy for designing artificial enzymes with high peroxidase-like activity, indicating potential for biosensing applications.
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JOURNAL OF ETHNOPHARMACOLOGY
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2026
·
IF 5.4
Xing-lan Ye,Ling-shuai Zeng,Qiang Liu,Wen-cheng Xu,Hong Liu,Hong Cheng,Wen-xiao Zheng,Xiao-qin Wang,Xue Xue
Ethnopharmacological relevance Huaiqihuang granule (HQH) is a traditional Chinese herbal formula comprising Trametes robiniophila Murr. , Lycii Fructus ., and Polygonatum sibiricum Red. This granule can nourish Qi and Yin, improve blood circulation and remove blood stasis. HQH has been extensively used to improve immune-mediated kidney diseases, such as IgA vasculitis nephritis (IgAVN). However, its renal protective mechanism remains elusive. Aim of the study This study investigated whether HQH ameliorates IgAVN by modulating the AMPK/mTOR-mediated autophagy pathway. Materials and methods The IgAVN mouse model was established using gliadin sensitization and India ink-mediated reticuloendothelial blockade (n = 10/group). The model mice were then treated for 4 weeks with low/high-dose HQH (4/8 g/kg/day) or dexamethasone (2.5 mg/kg/day). For in vitro analyses, human mesangial cells (HMCs) were first stimulated with aggregated IgA1 (aIgA1, 25 μg/mL) and then treated with the optimal concentration of HQH-containing serum (40 %). Furthermore, biochemical indices assays, renal histopathology, and immunofluorescence analysis of IgA deposition were performed. Moreover, autophagy levels were evaluated by assessing the expression of autophagy-related proteins, MDC staining, and mCherry-EGFP-LC3 transfection. Western blot and immunofluorescence were carried out to assess the levels of the AMPK/mTOR pathway proteins and proliferation markers. Results The results showed that HQH treatment significantly reduced 24-h urinary protein and serum IgA levels, but improved renal histopathology in IgAVN mice. Furthermore, HQH activated autophagy by upregulating p-AMPKα, LC3-Ⅱ/LC3-Ⅰ, and Beclin1, while inhibiting p-mTOR/mTOR and p62. Moreover, in HMCs, 40 % HQH-containing serum optimally inhibited aIgA1-induced mesangial proliferation and increased autophagic levels, as evidenced by the quantitative increase in autophagy activity and mean fluorescence intensity of MDC. However, these effects were blocked by AMPK inhibition. Conclusion This study showed that HQH alleviates IgAVN by upregulating AMPK/mTOR-mediated autophagy, inhibiting mesangial cell proliferation, and mitigating renal injury. These findings suggest that HQH has therapeutic potential for IgAVN and link autophagy modulation to IgAVN treatment.
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DYES AND PIGMENTS
·
2026
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IF 4.2
Jiahui Cai,Yuwei Du,Huimin Miao,Yingge Cong,Minghui Zan,Ruhong Yan,Li Li,Juan Yue
The overuse of tetracycline (TC) antibiotics has raised significant environmental and health concerns, while traditional detection methods face challenges in real-time monitoring and intracellular analysis. In this study, sulfur-doped carbon dots (S-CDs) were synthesized through a one-step hydrothermal method, using biotin and o -phenylenediamine as precursors. The resulting S-CDs exhibit three key functional properties: (1) a highly sensitive fluorescent quenching response to TC, with a detection limit of 76.6 nM and a linear range of 0–200 μM, driven by specific molecular interactions (inner filter effect and static quenching); (2) pH-dependent fluorescence signaling (pH 2.0–8.0) attributed to protonation of surface acid-base groups; and (3) selective lysosomal targeting, with a Pearson correlation coefficient of 0.95, confirmed by confocal microscopy in HeLa cells and zebrafish models. The S-CDs demonstrated excellent recoveries (97.07–107.99 %) in real food samples (milk, beef, and pork) and exhibited negligible cytotoxicity. This work effectively integrates environmental pollutant detection with intracellular microenvironment analysis, providing a versatile tool for investigating TC-induced lysosomal dysfunction and advancing food safety monitoring and biomedical imaging.
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ACS Nano
·
2026
·
IF 16
Minghao Jin,Muge Gu,Keyu Kong,Wenxuan Fan,Sonu Ng,Yuehao Hu,Zhe Wang,Jiemao Su,Wei-En Yuan,Huiwu Li,Zanjing Zhai
Prosthesis-associated osteolysis (PAO), driven by a self-perpetuating inflammatory-osteoclastogenic cycle, remains a critical challenge following arthroplasty. Current single-target therapies inadequately address the spatiotemporal heterogeneity of pathological microenvironments: ROS overload during the early immune activation phase and low pH in the osteoclastic resorption phase. This study develops bovine serum albumin (BSA)-coated copper–manganese carbonate nanocomposites (CuMnCO3@BSA, CMC) that dynamically coordinate immunosuppressive intervention with osteoclast-specific cuproptosis induction through microenvironment-guided functional switching. In vitro studies demonstrate that at neutral pH, Mn2+-mediated SOD/CAT-like nanozyme activity effectively scavenges ROS and reprograms macrophage metabolism to oxidative phosphorylation, thereby suppressing M1 polarization. During late osteoclast differentiation, acid-triggered carbonate decomposition releases Cu2+, selectively eliminating mature osteoclasts via cuproptosis-mediated mitochondrial lipoylated protein aggregation and TCA cycle collapse. In titanium particle-induced osteolysis models, this dual-functional CMC strategy demonstrates superior therapeutic efficacy to bisphosphonates. This study pioneers a microenvironment-adaptive nanotherapeutic approach, innovatively coupling cuproptosis with immune-metabolic regulation, thereby establishing a novel paradigm for osteolytic disease management.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.7
Yehui Kang,Yahui Zhang,Weijiao Fan,Liya Yu,Mengdie Yu,Yuejia Ding,Yu Cai,Aihong Jiao,Jiajie Xu
Head and neck squamous cell carcinoma (HNSCC) remains a clinically challenging malignancy due to limited therapeutic options and considerable treatment-related toxicity. In this study, we present an innovative nanotherapeutic platform—monocarboxylate transporter 1 (MCT1) antibody-targeted nanoparticles co-loaded with a second near-infrared window (NIR-II, 1000–1700 nm) absorbing conjugated polymer (PDTP-TBZ-OD, denoted as PTO) and emodin (MCT@PTO-Em NPs) —that synergistically integrates emodin-induced pyroptosis with NIR-II mediated mild photothermal therapy (MPTT) to address critical limitations of current HNSCC treatments strategies. The synthesized NIR-II-absorbing conjugated polymer PTO exhibits strong absorption at 1064 nm and a high photothermal conversion efficiency (39 %), enabling deeptissue penetration and precise hyperthermia control. Accordingly, MCT@PTO-Em NPs implement a dual-modal therapeutic strategy that triggers pyroptosis through NLRP3 inflammasome activation while simultaneously enhancing tumor cell death via mild hyperthermia. Moreover, emodin encapsulated within the nanoparticles (NPs) inhibits heat-shock protein 70 (HSP70), thereby disrupting cellular thermoresistance mechanisms and sensitizing cancer cells to MPTT. Additionally, surface functionalization of the nanoparticles with MCT1-targeting antibodies enhances tumor-specific delivery and intracellular accumulation, thereby minimizing off-target effects. Both in vitro and in vivo studies demonstrate that MCT@PTO-Em NPs achieve superior tumor suppression through the synergistic interplay between pyroptosis and MPTT, while exhibiting excellent biocompatibility. This work presents a precision nanomedicine strategy that integrates molecular targeting, pyroptosis-mediated immune activation, and MPTT, offering a transformative therapeutic paradigm for HNSCC and other solid tumors.
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Theranostics
·
2026
·Vol. 16
·期 1
·398
·
IF 13.3
Jian Xu,Susu Zhang,Yu Yang,Xingwei Wei,Yunteng Fang,Zhilin Wang,Linwen Lan,Jiayi Shen,Enqian Liu,Wuming Hu,Tingting Hu,Chaojie Yu,Ruizheng Liang,Lingchun Lyu
Rationale: Myocardial ischemia-reperfusion (MI/R) injury induces apoptosis, metabolic dysregulation, and ventricular remodeling through complex pathological mechanisms. Although nanozyme engineering has the potential for antioxidation, reoxygenation, and pro-vascularization, achieving responsive modulation of the pathological microenvironment remains significantly challenging. Methods: A layered double hydroxide (LDH)-based nanozyme (ZnSrMo-LDH/Cu) was synthesized via a low-temperature hydrothermal/isomorphic substitution method for MI/R treatment. The reactive oxygen species (ROS) scavenging ability and responsive ion release performance of ZnSrMo-LDH/Cu were evaluated through various spectroscopic characterization methods. The biosafety and therapeutic efficiency of ZnSrMo-LDH/Cu-BSA nanozymes were assessed by in vitro and in vivo experiments. Results: ZnSrMo-LDH/Cu demonstrated cascade superoxide dismutase (SOD) and catalase (CAT) activities, effectively overcoming acidic microenvironment limitations to maintain CAT activity rather than peroxidase (POD) activity while scavenging ROS to generate oxygen, with a ROS scavenging capacity 2.97 times that of Fe 3 O 4 . Moreover, the acid-triggered Sr 2+ release promoted vascular regeneration and synergistically improved the ischemic-hypoxic microenvironment. Consequently, after bovine serum albumin (BSA) modification, ZnSrMo-LDH/Cu-BSA demonstrated excellent cytoprotective effects, reducing the cardiomyocyte apoptosis rates to 9.4% ( in vitro ) and 20.7% ( in vivo ) of the levels in the MI/R group. In vivo studies further validated that ZnSrMo-LDH/Cu-BSA enhanced cardiac function and attenuated ventricular remodeling by inhibiting oxidative stress and promoting angiogenesis. Mechanistically, ZnSrMo-LDH/Cu-BSA provided a cardioprotective effect by inhibiting the TGF-β signaling pathway, thereby alleviating cell damage caused by MI/R. Conclusions: The pathologically responsive LDH-based nanozyme represents a promising avenue for MI/R treatment.
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DRUG DELIVERY
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2013
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IF 2.015
Zhipeng Chen,Dan Liu,Jian Wang,Li Wu,Weidong Li,Jun Chen,Bao-Chang Cai,Haibo Cheng
To increase the intra-articular (IA) retention time of osteoarthritis drugs in the synovial cavity and slow down the burst release of microspheres (MPs), we prepared a novel drug delivery system named nanoparticles-in-microspheres (NiMs). The system was constructed by dispersing the brucine-loaded nanoparticle, which was prepared by an emulsification method in the MPs. The NiMs were characterized by scanning electron microscope, Fourier transform infrared spectra and differential scanning calorimetry. After investigating the biocompatibility with synovium of NiMs in rats, the pharmacokinetics was studied and FX-imaging was used to visualize the transmission of nanoparticles after IA administration in rats. From the results, we know that the NiMs were spherical, there was no chemical bond between the drug and the polymer, and the drug was dispersed in the polymer in an amorphous form. Compared with MPs (41%), the burst release of NiMs could be slowed down to 9%. After that, the drug was released from NiMs by diffusion. The results of FX imaging in rats showed that the NiMs could stay in the articular cavity for over 11 d. The studies of pharmacokinetics revealed that the NiMs could slow down the burst release and improve retention in vivo. This study demonstrates the feasibility of using NiMs to slow down the burst release and increase the retention of therapeutic agents in articular joints.
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DESIGNED MONOMERS AND POLYMERS
·
2014
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IF 2.21
Bo Gao,Ping Guan,Xiaoling Hu,Liwei Qian,Qiaoli Wang,Longfei Yang,Dan Wang
L-Phenylalanine (L-Phe) imprinted polymers (L-MIPs) were prepared via precipitation polymerization using 1-vinyl-3-carboxymethylimidazolium chloride ([VCIM]Cl) as functional monomer, which could interact with L-Phe through electrostatic interactions, hydrogen-bonding, and π–π interactions. The morphologies of obtained polymers were observed by a scanning electron microscope, and their structures were analyzed by Fourier transform infrared spectroscopy. The effects of monomer species and molar ratio of template to functional monomer or crosslinker on adsorption capacity and imprinting factors (IFs) of polymers were investigated. The result indicated that [VCIM]Cl was preferable monomer and the optimum molar ratio of template to functional monomer and crosslinker was 1:4:16. The maximum IF of the obtained L-MIPs could reach 3.86 in 0.4 mg mL−1 L-Phe solution at 25 °C, after 8 h of adsorption and the maximum adsorption capacity of L-MIPs for L-Phe was 33.08 mg g−1. The adsorption behavior of L-Phe on the L-MIPs was construed seriously within the experimental concentration by Freundlich model and Langmuir model. In terms of the higher correlation coefficient, it was considered that the adsorption behavior of L-Phe fit Freundlich model better than Langmuir model, which means that the distribution of active sites was heterogeneous in L-MIPs. The selectivity adsorption showed L-MIPs have better specificity and selectivity for L-Phe than that of L-His and L-Trp. Finally, L-MIPs were applied to the CD and Automatic Polarimeter experiments, showing that L-MIPs had the capability of specific chiral recognition for L-Phe.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
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IF 5.4
Li Wang,Jiaqi Chen,Simin Gou,Yuting Long,Dandan Yang,Jifang Zhou,Yaxin Zheng,Wan Yan,Xuzhi Hu
Postoperative tumor recurrence and chemoresistance in osteosarcoma treatment highlight the urgent need to develop localized implants, particularly large-scale scaffolds capable of selectively eliminating residual tumor cells, minimizing significant trauma during implantation. In this study, a drug-loaded intelligent scaffold system was designed for minimally invasive surgical implantation, enabling localized sustained release of the anticancer drug paclitaxel (PTX). This material system was fabricated into a three-dimensional porous scaffold via in situ polymerization combined with gas foaming technology. Citrate-functionalized amorphous calcium phosphate (CACP) was incorporated into the polyurethane (PU) scaffold, serving as an antibacterial agent while enhancing osteogenic expression. Following surface modification with polydopamine (PDA), PTX-Lips were covalently immobilized onto the modified scaffold surface. The resulting scaffold exhibited an extracellular matrix-mimicking highly porous structure, provided good mechanical support, and demonstrated excellent shape memory properties at 37°C. Comprehensive in vitro and in vivo studies confirmed that the drug-loaded scaffold exhibited three key functionalities: sustained drug release kinetics; significant tumor cell inhibitory efficacy; and biocompatibility supporting normal cell adhesion. Crucially, animal models unequivocally demonstrated its effectiveness in preventing postoperative local tumor recurrence and suppressing distant metastasis. This scaffold provided an efficient synergistic strategy for postoperative osteosarcoma treatment by integrating localized chemotherapy with tissue regeneration support.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Xinlan Wang,Xiong Wu,Menghan Qi,Huasheng Lai,Jianlian Xie
A “signal-off” surface-enhanced Raman scattering (SERS) strategy is developed for the rapid and ultrasensitive detection of total free arsenic in water and traditional Chinese medicine (TCM) beverages. The method employs methimazole (MMI) as a multifunctional probe that first reduces As(V) to As(III) and subsequently forms a As-MMI complex, leading to concentration-dependent attenuation of the characteristic MMI SERS signal. The assay achieves rapid equilibration within 2 min under optimized conditions and exhibits exceptional selectivity for free arsenic over common interfering ions, attributed to the combined redox and As-S complexation mechanisms under acidic pH. Ultrasensitive detection is achieved with a limit of detection of 0.6 fmol/L, more than eight orders of magnitude below the WHO safety guideline for drinking water. Practical applicability is demonstrated through quantitative recovery (93.8–106%) of spiked arsenic in real water and TCM beverage samples with relative standard deviations below 6.1%. This work provides a rapid, selective, and ultra-sensitive platform for free arsenic monitoring in environmental and pharmaceutical quality control applications.
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危险品化学品经营许可证（带存储）