COMPOSITES SCIENCE AND TECHNOLOGY
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2016
·
IF 3.897
Ailin Gao,Qing Yang,Lixin Xue
The crystallinity of semi-crystallized Poly ( l -Lactic acid) (PLLA) is low, especially as a porous membrane prepared from non-solvent induced phase separation (NIPS) process. To improve the crystallinity and thermal stability of PLLA porous membranes, hexafluoroisopropanol (HFIP) was added into the PLLA casting solution together with a small amount of Silk Fibroin (SF). It is found that by optimizing the additive amounts of HFIP and SF, PLLA composite membranes with improved crystallinity (up to 47.1%) and endurance to temperature (above 90 °C) could be prepared with comparable mechanical property to the virgin PLLA membranes. The effects of HFIP in NMP solution on PLLA during the NIPS process was further investigated using dynamic rheological methods relating to the crystallinity of the formed PLLA porous membranes.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2016
·Vol. 51
·期 9
·1988-1996
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IF 1.504
Zhi-Hong Zhang,Lang-Hong Wang,Xin-An Zeng,Charles S. Brennan,Margaret Brennan,Zhong Han
Summary In this work, the effect of pulsed electric fields (PEF) treatment on the interactions between amino acids (using L-Phenylalanine: L-Phe) and sugar (using β-Cyclodextrin: β-CD) complex was analysed by fluorescence spectroscopy, Raman spectroscopy and simultaneous thermal analyzer. Moreover, the molecular dynamics of β-CD–L-Phe inclusion complex treated by PEF was calculated by molecular modelling. The results indicated that β-CD–L-Phe complexes are formed by a molar ratio of 1:1, and the stability constant of such complexes increased from 147 to 614 M −1 by PEF treatment. Thermal characterisations of β-CD–L-Phe complexes indicated that the PEF treatment could increase the yield of complexes. The PEF treatment resulted in an increase in the reaction enthalpy of β-CD–L-Phe inclusion complexes by DSC curve. These results show that PEF treatment has the potential to promote the chemical processing, especially the small organic molecules participate in inclusion or cross-linking reaction.
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ACS Applied Materials & Interfaces
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2016
·
IF 7.145
Xiaoyan Zhou,Qianqian Zheng,Changyong Wang,Jiake Xu,Jian-Ping Wu,Thomas Brett Kirk,Dong Ma,Wei Xue
The drug/gene codelivery is a promising strategy for cancer treatment. Herein, to realize the codelivery of docetaxel and MMP-9 siRNA plasmid efficiently into tumor cells, a star-shaped amphiphilic copolymer consisting of hyperbranched polyglycerol derivative (HPG-C18) and dendritic poly(l-lysine) (PLLD) was synthesized by the click reaction between azido-modified HPG-C18 and propargyl focal point PLLD. The obtained HPG-C18-PLLD could form the nanocomplexes with docetaxel and MMP-9, and the complexes showed good gene delivery ability in vitro by inducing an obvious decrease in MMP-9 protein expression in MCF-7 cells. The apoptosis assay showed that the complex could induce a more significant apoptosis to breast cancer cells than that of docetaxel or MMP-9 used alone. In vivo assay indicated that the codelivery strategy displayed a better effect on tumor inhibition. Moreover, HPG-C18-PLLD displayed lower toxicity as well as better blood compatibility compared to polyethylenimine PEI-25k, which may be the result of that HPG-C18-PLLD showed the comparative MMP-9 delivery ability in vivo compared with PEI-25k even if it showed the slight lower transfection efficiency in vitro. Therefore, HPG-C18-PLLD is a safe and effective carrier for the codelivery of drug/gene, which should be encouraged in tumor therapy.
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Food & Function
·
2016
·Vol. 7
·期 11
·4683-4692
·
IF 2.686
Fan Yuan-jing,Wei Wei,Luo Jian-ping,Jin Yu-xia,Dai Zi-ling
This study aimed to explore the effects of genistein on regulating the activation of UGTs via the Nrf2/Keap1 pathway and to elucidate the underlying mechanisms of detoxification and hepatic protection. Experiments monitoring genistein-induced protection against acetaminophen-induced cell damage were performed in L-02, HepG2 and Hep3b cells. The results of the MTT, AST, ALT, LDH, GSH and GSSG assays showed that genistein evidently protected the cells from acetaminophen-induced injury in a dose-dependent manner. The control cells were treated with 10 mM acetaminophen without genistein to compare with the effects of the combination of acetaminophen and genistein on the expression of UGT1A1, 1A6 and 1A9, Nrf2 and Keap1 mRNAs, as well as the expression of Nrf2 and Keap1 proteins, which were tested by western blotting. The results showed that the expression of the Nrf2 mRNA and protein increased; in contrast, the expression levels of the Keap1 mRNA and protein were obviously reduced by genistein in a dose-dependent manner. Meanwhile, the expression of the UGT mRNA was increased, and UGT1A9 exhibited the highest expression among the three UGTs. Accordingly, the residual acetaminophen content was obviously reduced and acetaminophen glucuronidation increased after 24 hours of treatment with genistein in a dose-dependent manner.
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Microorganisms
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2024
·Vol. 12
·期 8
·1624
·
IF 4.1
Yingsheng Liu,Yizhuo Du,Yue Li,Chuang Li,Shanshan Zhong,Zhelun Xu,Congyan Wang,Daolin Du
The deposition of nitrogen in soil may be influenced by the presence of different nitrogen components, which may affect the accessibility of soil nitrogen and invasive plant–soil microbe interactions. This, in turn, may alter the success of invasive plants. This study aimed to clarify the influences of the invasive plantBidens pilosaL. on the physicochemical properties, carbon and nitrogen contents, enzymatic activities, and bacterial communities in soil in comparison to the native plantPterocypsela laciniata(Houtt.) Shih treated with simulated nitrogen deposition at 5 g nitrogen m−2yr−1in four forms (nitrate, ammonium, urea, and mixed nitrogen). MonoculturalB. pilosaresulted in a notable increase in soil pH but a substantial decrease in the moisture, electrical conductivity, ammonium content, and the activities of polyphenol oxidase, β-xylosidase, FDA hydrolase, and sucrase in soil in comparison to the control. Co-cultivatingB. pilosaandP. laciniataresulted in a notable increase in total soil organic carbon content in comparison to the control. MonoculturalB. pilosaresulted in a notable decrease in soil bacterial alpha diversity in comparison to monoculturalP. laciniata. Soil FDA hydrolase activity and soil bacterial alpha diversity, especially the indices of Shannon’s diversity, Simpson’s dominance, and Pielou’s evenness, exhibited a notable decline under co-cultivatedB. pilosaandP. laciniatatreated with nitrate in comparison to those treated with ammonium, urea, and mixed nitrogen.
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ACS Agricultural Science & Technology
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2024
·
IF 2.3
Samera Ali Al-Gahtany,Abeer S. Meganid,Dalal Mohamed Alshangiti,Sheikha A. Alkhursani,Mohamed Mohamady Ghobashy,Mohamed A. Amin,Tasneam K. El-Damhougy,Atheer S. Almutairi,Mohamed Madani
Drought stress significantly affects plant growth and productivity, including sunflowers (Helianthus annuus L.). Superabsorbent hydrogels, specifically composed of dextrin and polyacrylamide, offer a potential solution to mitigate drought stress and enhance the biochemical traits of sunflowers. This study explored the impact of copolymer composition on the gel fraction, swelling behavior, water retention, morphology, and chemical structure of dextrin (Dix)/polyacrylamide (PAAm) hydrogels synthesized via radical polymerization. Results revealed that the 50/50 ratio of the Dix/PAAm hydrogel exhibited desirable characteristics, including a heterogeneous pore structure (approximately 100 μm) and higher water absorption capacity attributed to a greater Dix content. In a pot experiment, the (Dix/PAAm) hydrogel played a crucial role in alleviating the harmful effects of drought stress on the growth, chlorophyll content, fresh shoot weight, and pigments in sunflowers. The hydrogel application positively influenced these parameters by enhancing water retention, nutrient availability, and physiological responses to drought stress. Overall, these findings highlight the potential of the (Dix/PAAm) hydrogel as an effective tool for enhancing plant resilience to drought stress and promoting growth and biochemical traits in sunflowers.
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Metabolites
·
2024
·Vol. 14
·期 4
·176
·
IF 3.4
Yuan Jing,Wei Chen,Xuebai Qiu,Shuyue Qin,Weichang Gao,Chaochan Li,Wenxuan Quan,Kai Cai
The quality of crops is closely associated with their geographical location and yield, which is reflected in the composition of their metabolites. Hence, we employed GC–MS pseudotargeted metabolomics to investigate the metabolic characteristics of high-, medium-, and low-yieldNicotiana tabacum(tobacco) leaves from the Bozhou (sweet honey flavour) and Shuicheng (light flavour) regions of Guizhou Province. A total of 124 metabolites were identified and classified into 22 chemical categories. Principal component analysis revealed that the geographical location exerted a greater influence on the metabolic profiling than the yield. Light-flavoured tobacco exhibited increased levels of sugar metabolism- and glycolysis-related intermediate products (trehalose, glucose-6-phosphate, and fructose-6-phosphate) and a few amino acids (proline and leucine), while sweet honey-flavoured tobacco exhibited increases in the tricarboxylic acid cycle (TCA cycle) and the phenylpropane metabolic pathway (p-hydroxybenzoic acid, caffeic acid, and maleic acid). Additionally, metabolite pathway enrichment analysis conducted at different yields and showed that both Shuicheng and Bozhou exhibited changes in six pathways and four of them were the same, mainly C/N metabolism. Metabolic pathway analysis revealed higher levels of intermediates related to glycolysis and sugar, amino acid, and alkaloid metabolism in the high-yield samples, while higher levels of phenylpropane in the low-yield samples. This study demonstrated that GC–MS pseudotargeted metabolomics-based metabolic profiling can be used to effectively discriminate tobacco leaves from different geographical locations and yields, thus facilitating a better understanding of the relationship between metabolites, yield, and geographical location. Consequently, metabolic profiles can serve as valuable indicators for characterizing tobacco yield and geographical location.
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FITOTERAPIA
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2024
·
IF 2.5
Songqin Yang,Mao Zhao,Yuhan Feng,Xia Zhang,Qiuhong Li,Wenwen Jiang,Daoping Wang
Toddalia asiatica (L.) Lam. (TA) is a traditional folk medicine of ethnic minorities in the southwest of China. It is widely used in the treatment of dispersing blood stasis and activating blood. However, the effective substance and pharmacological mechanism have not been fully elucidated. The zebrafish larvae were treated with Phenylhydrazine (PHZ) to establish a thrombus model, and the staining intensity of zebrafish red blood cells was analyzed. The antithrombotic activity of TA was verified for the first time, and it was found that the inhibition rate of TA on thrombosis was up to 60.85 %. The chemical ingredients of TA were collected by combining UPLC-HRMS analysis and the literature research. Network pharmacology revealed that six key targets were obtained, which including TNF, AKT1, EGFR, PTGS2, PPARG, and IFNG. It showed that the PI3K-Akt pathway was a core signaling pathway. Coagulation factor III(TF), playing an important role in the process of hemostasis and thrombosis, which ranks high in the PPI network. Moreover, the results of molecular docking showed that the active components had a strong binding force with TF, which indicated that TF might be the key target of TA in treating thrombosis. In vitro experiments showed that TA could inhibit TNF-α-induced high expression of TF in EA.hy926 cells. In addition, TA could inhibit TNF-α-activated expression of Akt, IκBα and P65 protein phosphorylation in PI3K-Akt pathway. The results showed that TA had antithrombotic activity and exerted an antithrombotic effect by inhibiting the expression of TF through the PI3K-Akt-NF-κB signaling pathway.
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Foods
·
2024
·Vol. 13
·期 8
·1240
·
IF 4.7
Min Yu,Beibei Wang,Zhiqiang Huang,Jinjiao Lv,Yunfei Teng,Tianbo Li,Yu Zhang,Kun Dong,Dong Qin,Junwei Huo,Chenqiao Zhu
This study aims to comprehensively investigate the effects of hot-air dehydration on the quality of blue honeysuckle berries (Lonicera caeruleaL.). The results demonstrated that drying with hot air at 40–65 °C for 7–72 h resulted in blue honeysuckle berries with a moisture content of 0.21–1.10 g H2O/g dry weight. Generally, low to medium temperatures (40–55 °C) showed a better effect on the quality than high temperatures (60–65 °C). Specifically, drying at 40 °C exclusively resulted in better retention of cuticular wax, the best sensory appearance, and the highest total phenolic content. Drying at 45 °C and 50 °C resulted in the highest antioxidant capacity and the optimal sensory flavor. Drying at 55 °C led to the highest soluble solid/acid ratio, ascorbic acid concentration, total flavonoid, and total anthocyanin. The work introduces an innovative raw berry product and provides a comprehensive practical and theoretical framework for convective dehydration of blue honeysuckle berries.
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MOLECULAR NUTRITION & FOOD RESEARCH
·
2024
·
IF 4.5
Dai Zhao,Yuan Gao,Yiqin Chen,Yingsi Zhang,Yian Deng,Sai Niu,Hanchuan Dai
Scope Oxidative stress caused by iron overload tends to result in intestinal mucosal barrier dysfunction and intestinal microbiota imbalance. As a neutral and nonprotein amino acid, L-Citrulline (L-cit) has been implicated in antioxidant and mitochondrial amelioration properties. This study investigates whether L-cit can alleviate iron overload-induced intestinal injury and explores the underlying mechanisms. Methods and results C57BL/6J mice are intraperitoneally injected with iron dextran, then gavaged with different dose of L-cit for 2 weeks. L-cit treatment significantly alleviates intestine pathological injury, oxidative stress, ATP level, and mitochondrial respiratory chain complex activities, accompanied by ameliorating mitochondrial quality control. L-cit-mediated protection is associated with the upregulation of Glutathione Peroxidase 4 (GPX4) expression, inhibition Nuclear Receptor Coactivator 4 (NCOA4)-mediated ferritinophagy and ferroptosis, and improvement of gut microbiota. To investigate the underlying molecular mechanisms, Intestinal Porcine Epithelial Cell line-J2 (IPEC-J2) cells are treated with L-cit or AMP-activated Protein Kinase (AMPK) inhibitor. AMPK signaling has been activated by L-cit. Notably, Compound C abolishes L-cit's protection on intestinal barrier, mitochondrial function, and antioxidative capacity in IPEC-J2 cells. Conclusion L-cit may restrain ferritinophagy and ferroptosis to regulate iron metabolism, and induce AMPK pathway activation, which contributes to exert antioxidation, ameliorate iron metabolism and mitochondrial quality control, and improve intestinal microbiota. L-cit is a promising therapeutic strategy for iron overload-induced intestinal injury.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Weijie Gao,Zijian Jing,Yifang Meng,Qinghai Liu,Hualei Wang,Dongzhi Wei
Lysine cyclodeaminase (LCD)-mediated synthesis of l-pipecolic acid (l-PA) from l-lysine (l-Lys) is a promising approach. However, only one LCD has been reported, and its inadequate activity limits industrial applications. To address this problem, a substrate analogue-guided enzyme mining strategy was employed. A novel ornithine cyclodeaminase (OCD) from Rhizobium leguminosarum (RlOCD) was identified in combination with directed macrogenomic approaches. RlOCD displayed a conversion rate of 28% at a substrate loading as high as 1000 mM. A multiregion synergy strategy consisting of pocket reshaping, dynamical cross-correlation matrix-guided coevolutionary design, and surface modification was used to design RlOCD from the inside-out. A quadruple mutant (V93C/L119C/I170T/R90L) designated Mu4 with significantly increased activity was obtained, which showed a 28.46-fold increase in the catalytic efficiency. The conversion of Mu4 was 91% within 10 h at 1000 mM (146.19 g L–1) loading. The space–time yield of 282.1 g L–1 d–1 is the highest level ever reported. Molecular dynamics simulations and interaction analyses revealed that efficient pocket expansion and unique conformational rearrangements increased the affinity for the substrate, resulting in a more catalytically active conformation. This study expands the toolbox for the production of l-PA and demonstrates the effectiveness and potential of Mu4 for its production.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Xiangchen Su,Xinrong Geng,Fang Li,Mingzhu Song,Ruizhen Lv,Yifei Zhang,Jiayu Yuan,Jia Dong,Yijie Shi,Liang Zhao
Due to the high-glucose environment of diabetic wounds, a significant proliferation of bacteria can occur, resulting in an inflammatory response that extends the inflammatory phase of the wound, thereby complicating the healing process in diabetic wounds. Eliminating the proliferation of bacteria plays a crucial role in promoting the healing of diabetic wounds. Under near-infrared (NIR) laser irradiation, l -arginine-modified natural product puerarin (Pue)-derived carbon nanoparticles ( l -Arg-CNP) not only exhibited excellent photothermal effects but also produced reactive oxygen species (ROS) to react with l -arginine ( l -Arg) for producing Nitric Oxide (NO), thus contributing to a synergistic antibacterial therapy in diabetic wound. At the same time, l -Arg-CNP retained Pue’s original characteristics to promote cell proliferation and angiogenesis. Following the loading of l -Arg-CNP into microneedle patches ( l -Arg-CNP@MN), it can deliver them into the deeper wound, effectively killing bacteria, reducing inflammatory infiltration, and promoting neovascularization at the wound site. It offers an effective therapeutic strategy for treating diabetic wound healing.
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