FOOD HYDROCOLLOIDS
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2024
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IF 11
Xumei Feng,Xixi Wu,Tian Gao,Mengjie Geng,Fei Teng,Yang Li
In this study, carboxymethyl cellulose (CMC) was introduced to reduce the instability and poor functional properties of soy protein isolate (SPI) in low pH environment. The SPI–CMC systems containing 0–0.5% CMC were prepared at pH 4.0 and 5.0. By exploring the interaction mechanism between them, the optimal formation conditions of the composite systems were revealed. The results showed that CMC was continuously adsorbed on the surface of SPI at pH 4.0, in which electrostatic behavior and hydrogen bonds played an important role. The emulsion properties were the best at 0.4% CMC, and the emulsion activity index (EAI) and emulsion stability index (ESI) were increased by 48.91 m 2 /g and 91.41 min compared with SPI emulsion. The optical microscope images showed that the emulsion interfacial proteins were formed and the droplets were evenly distributed at 0.4% CMC. At pH 5.0, the stability of the SPI–CMC systems was highly dependent on the steric hindrance and high viscosity of CMC. The EAI and ESI of the emulsion at 0.5% CMC were the highest, which increased by 49.08 m 2 /g and 83.79 min compared with SPI emulsion. The optical microscope images confirmed that the addition of 0.5% CMC significantly inhibited the leakage of oil droplets. In conclusion, this study provides an effective strategy for stabilizing SPI emulsion in low pH environments, which has important practical application value.
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高影响因子
FOOD HYDROCOLLOIDS
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2024
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IF 11
Hanyu Li,Minghui Zhao,Jinghong Li,Jiaqi Wang,Huajiang Zhang,Jing Wang,Ning Xia,Zhongjiang Wang,Ahmed M. Rayan
The pH-driven method, recognized for its material safety and avoidance of organic solvents, is considered a promising approach for the encapsulation of hydrophobic active substances. However, the interaction mode and mechanism between carriers and receptors in two stages (alkalization and neutralization) remain underexplored. In this study, the hydrophilic globular protein ovalbumin (OVA) was utilized as a carrier to load curcumin (Cur) by the pH-driven method, with an alkalization pH of 8–12. The results indicated that the solubility of Cur and the degree of protein unfolding increased with increasing alkalization pH, with a transformation in the secondary structure observed at pH 12. During the alkalization stage at pH 11 and 12, the loading efficiency of Cur exceeded 90%, with binding constants reaching as high as 5.79 × 10 5 M −1 and 1.44 × 10 5 M −1 , respectively. The interactions transitioned through three phases in the alkalization process: electrostatic dominance to hydrophobic dominance, electrostatic assistance to hydrophobic dominance, and electrostatic repulsion hindrance. Notably, the generation of electrostatic repulsion and the potential loss of binding sites resulted in a decrease in the binding affinity between OVA and Cur at pH 12. In the neutralization stage, a stable structure dominated by hydrogen bonds and van der Waals forces was observed, with Cur encapsulated within the hydrophobic core of OVA. The mechanism proposed in this study elucidates the factors affecting the efficacy of the pH-driven method and helps to refine the application in hydrophobic active substance delivery systems.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Hui Li,Shuangshuang Chen,Yumei Li,Zelin Sang,Zhenhua Chen,Xifan Mei,Xiuli Ren
In the pathogenesis of inflammatory bowel disease (IBD), overstimulation of inflammatory factors can trigger a coagulation cascade, increase the risk of intestinal micro-thrombosis and lead to microcirculation disorders. However, prevention of microcirculation pathways has not received enough attention. Heparin is commonly used in anticoagulant therapy, but oral delivery does not have an excellent anticoagulant effect. To improve the stability of heparin (HEP) in the gastrointestinal tract, zein/tea polyphenol nanospheres with a core-shell structure (EGNs@Z) were developed for oral administration of heparin (HEGNs@Z). The Zein shell has pH-responsive properties and is effective in preventing premature dissolution of heparin. At the same time, EGCG nanospheres (EGNs) play an anti-inflammatory role, jointly improve the vicious cycle between inflammation and microthrombosis. The results of SEM, TEM and FTIR showed that EGNs successfully encapsulated heparin and formed zein shells on the surface of microspheres with a thickness of 50-100 nm. In vitro simulated digestion experiments showed that zein shells prevented the breakdown of microspheres and heparin in a simulated gastric environment, whereas EGNs and HEP were slowly degraded and released in a simulated intestinal environment. Coagulation analysis showed that HEGNs@Z was effective in delaying clotting time. A mouse model of acute colitis has also shown that HEGNs@Z robust promotes colonic epithelial regeneration, inhibits malignant microcirculation, and reduces bleeding risk. These findings reveal that this orally bioavailable multifunctional material may provide a novel, effective and convenient treatment for inflammatory bowel disease.
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DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
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2024
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IF 5.8
Ying Liu,Qingyun Ma,Zhixuan Wang,Xinxin Shang,Yuting Lan,Guoping Guan,Lu Wang
Thrombosis and infection are the primary challenges limiting the clinical application of small-diameter artificial blood vessels. Numerous studies have focused on improving the hemocompatibility of artificial vascular materials, addressing properties such as anticoagulant activity, antithrombogenicity, low hemolysis rates, and reduced platelet adhesion. However, there has been comparatively less attention given to the antibacterial properties of these materials. This study utilizes polyester braided tubes and PVA/PAAm hydrogel materials, combined with surface modification techniques, to develop a pH-responsive antibacterial hydrogel artificial blood vessel graft. The hydrogel surface was grafted with a polymer brush hierarchical structure, and antimicrobial peptide MLT was immobilized to confer antibacterial properties. Additionally, 2,3-dimethylmaleic anhydride (DMMA) was covalently attached as a shielding group to achieve an environment-responsive strategy. The research demonstrates that this artificial blood vessel graft exhibits structural stability, excellent mechanical properties, and good cellular and hemocompatibility. Furthermore, it exhibits pH-responsive capabilities, triggering efficient antibacterial activity in low pH environments. The modified antibacterial hydrogel artificial blood vessel material shows low hemolysis rates, excellent anticoagulant properties, and reduced platelet adhesion. Therefore, the strategy of combining MLT with DMMA may effectively enhance the hemocompatibility and responsive antibacterial performance of artificial blood vessel materials.
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高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2014
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IF 3.785
Yuanyuan Ding,Jingtian Han,Baocheng Tian,Jian Han,Jing Zhang,Hong Zheng,Yuzhen Han,Meishan Pei
Smart nanoparticles based on the mechanisms of asialoglycoprotein (ASGP)-mediated endocytosis and pH-induced drug release were developed for the efficient treatment of hepatoma using a newly developed copolymer, methoxy-polyethylene glycols (PEG)-b-poly ( d -galactopyranose) (MPEG-b-PMaIPG). The particles exhibited spherical shapes, uniform particle size distribution (100 ± 4.43 nm), negative zeta potential (−32.8 ± 0.23 mV), high drug loading (24.77 ± 2.68%) and encapsulation efficiency (66.12 ± 9.44%). The in vitro drug release was also investigated, resulting that the release of drug from particles depended on different pH value. In vitro cell cytotoxicity and hemolysis assays were conducted to confirm the safety of the MPEG-b-PMaIPG nanoparticles. Anticancer activity showed that DOX-loaded MPEG-b-PMaIPG nanoparticles exhibited a high antitumor activity toward HepG2 cells, which was similar to free DOX, while blank MPEG-b-PMaIPG nanoparticles were non-toxic up to a tested concentration of 1.0 mg/mL. Confocal laser scanning microscopy (CLSM) and flow cytometry (FCM) were used to verify the targeting efficiency of d -galactopyranose-modified nanoparticles. The results clearly demonstrated that d -galactopyranose-modified nanoparticles were taken up quickly by the HepG2 cells, which suggests that MPEG-b-PMaIPG nanoparticles with good biocompatibility and non-toxic for normal cells may be used as an effective cancer-targeting drug delivery system for chemotherapy.
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PubMed
高影响因子
Acta Biomaterialia
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2019
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IF 6.638
Xiaohui Pu,Luling Zhao,Jia Li,Rufeng Song,Yanling Wang,Keke Yu,Xianqiao Hou,Peng Qiao,Lanlan Zong,Sheng Chang
Amphiphilic poly(ethylene glycol)-imino-poly(benzyl- l -aspartate) (PIPA) and poly(ethylene glycol)-poly(benzyl- l -aspartate) (PPA) block copolymers were synthesized as pH-responsive and pH-nonresponsive copolymers, respectively. Polymer micelles were fabricated by the film dispersion method, and hydroxycamptothecin (HCPT) was physically encapsulated into the micelles. The average diameter of the HCPT-loaded PIPA micelles (PIPAH micelles) was approximately 230 nm, which was slightly smaller than that of the HCPT-loaded PPA micelles (PPAH micelles, approximately 260 nm). The drug-loading content and encapsulation efficiency of the PIPAH micelles (3.33% and 68.89%, respectively) were slightly higher than those of the PPAH micelles (2.90% and 59.68%, respectively). The PIPAH micelles exhibited better colloid stability, storage stability, and plasma stability than the PPAH micelles. Drug release from the PIPAH micelles with imino groups was pH dependent, and more than 75% or 65% of the loaded HCPT was released within 24 h in weakly acidic media (pH 5.0 or 6.0, respectively). An in vitro cell assay demonstrated that the pH-sensitive micelles exhibited potent suppression of cancer cell proliferation and little cytotoxicity on normal cells. Additionally, these micelles could be efficiently internalized by the tumor cells through macropinocytosis- and caveolin-mediated endocytotic pathways. HCPT-loaded micelles had longer circulation time than the HCPT solution in a pharmacokinetic study. In vivo antitumor experiments indicate that the PIPAH micelles had better antitumor efficacy than the pH-insensitive PPAH micelles and the HCPT solution. Therefore, the pH-responsive PIPAH micelles have great potential for high-efficiency delivery of HCPT. Statement of Significance In this study, a new type of pH-responsive amphiphilic copolymer, poly(ethylene glycol)-imino-poly(benzyl- l -aspartate) (PIPA) block copolymer, was synthesized. This copolymer had then self-assembled to form nanomicelles for tumor intracellular delivery of hydroxycamptothecin (HCPT) for the first time. In in vitro test, the PIPAH micelles exhibited adequate stability and pH-dependent drug release. To one's excitement, the PIPAH micelles exhibited better antitumor efficacy and biosafety than the pH-insensitive micelles (PPAH) and the HCPT solution in in vitro and in vivo antitumor experiments. Therefore, the pH-responsive micelles in this study have significant potential to be used for high-performance delivery of HCPT and potentially for the targeted delivery of other cancer therapeutic agents. The polymer designed in this study can be used as a carrier of poorly soluble drugs or other active ingredients.
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DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2025
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IF 6.3
Meiyi Tang,Hou Tao,Hongli Lu,Yutong Xue,Meiqi Ren,Dan Cui
Fenton-based advanced oxidation processes had shown significant potential for antibiotics remediation, yet their application remained constrained by challenges including the dependence on continuous H 2 O 2 supplementation, limited catalyst reusability, and insufficient mechanistic understanding of antibiotic degradation. This study developed a pH-universal heterogeneous electro-Fenton system using an in-situ synthesized Fe@Fe 2 O 3 /active carbon fiber (ACF) composite cathode for sulfadiazine (SDZ) removal from wastewater. Under optimal conditions (pH of 2-3, current density of 20 mA/cm 2 , aeration rate of 0.6 L/min, and electrolyte concentration of 0.05 mol/L Na 2 SO 4 ), the removal efficiency of SDZ achieved 90.0 % ± 0.6 % and the corresponding removal rate was as high as 22.4 ± 0.1 g/(m 3 ·h). Notably, the Fe@Fe 2 O 3 /ACF composite cathode demonstrated exceptional stability with minimal iron leaching of 18.2 ± 0.6 μg/L in each cycle. Cross-scale mechanism validation, which integrated radical scavenging experiments, electron spin resonance spectroscopy, density functional theory, and intermediates analysis, revealed that hydroxyl radicals (·OH) was the exclusive reactive species driving SDZ degradation and that the preferential substitution of the free amino group and cleavage of the pyrimidine ring were critical steps in intermediate formation. This work advanced the mechanistic understanding of Fenton-based antibiotic degradation while providing a sustainable strategy for stable catalytic system design.
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Han Guo,Jingwei Kou,Houle Xiang,Hui Wang,Ting Guo,Hanjie Ying,Huanqing Niu,Jinglan Wu
Wastewater that contains highly toxic and carcinogenic organic dyes is harmful to the environment. Herein, a novel magnetic multifunctional pH-responsive adsorbent (Fe 3 O 4 @UiO-66-NH 2 /GSH) was developed, which showed excellent adsorption properties for both anionic and cationic dyes at different pH values. The adsorption capacities for CR (pH = 2) and MB (pH = 11) at 298.15 K were achieved at 1609.73 mg/g and 1827.24 mg/g, respectively. The effect of pH on the adsorption capacity was discussed experimentally and theoretically. Moreover, the uptake of four additional dyes (MO, AB, NR, and MV) was determined to test the application properties of the prepared particles. The influence of the salt concentration was also studied. The results showed that the prepared adsorbents can be applied in the treatment of complex dye wastewater. At the end of this work, the adsorption mechanism was investigated systematically by adsorption thermodynamics and hydrodynamics, as well as by comparing the FTIR and XPS results before and after adsorption. This demonstrated that electrostatic interactions played an essential role in the adsorption process. Last but not least, the regeneration rate of Fe 3 O 4 @UiO-66-NH 2 /GSH remained above 90 % after four consecutive adsorption-desorption cycles in 10 % and 20 % ethanol solutions, which indicated that it had good reusability and recyclability and was an economical material. Thus, the synthesized Fe 3 O 4 @UiO-66-NH 2 /GSH is a promising adsorbent that can be used for dye removal.
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高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
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2025
·
IF 2.8
Jiayao Wang,Jingguo Li,Shaokui Cao,Yingliang Liu,Jun Shi
In recent years, the remotely multi-responsive chitosan (CS) hydrogel has been garnering extensive attention owing to its remarkable potential in biomedical applications. Meanwhile, it presents a wide range of uses in the realm of controlled drug delivery and photothermal chemical combination therapy. In this paper, the CS/β-glycerophosphate disodium salt (β-GP) physical crosslinked network was employed as the framework to encapsulate near-infrared (NIR) responsive hollow copper sulfide (CuS)/polydopamine (PDA) nanoparticles, and the NIR/pH synergistic responsive CS/CuS/PDA hydrogel was fabricated. The negatively charged β-GP, along with the hollow CuS/PDA nanoparticles, played a key role in promoting the creation of abundant electronegative cavities. As a result, the hybrid hydrogel achieved an impressively high DOX loading rate, reaching up to 95.5%. The CS/CuS/PDA hydrogel exhibits excellent pH responsiveness, which could be attributed to the disintegration of the CS/β-GP network under acidic buffer solution. In addition, hollow CuS/PDA nanoparticles embedded in the hydrogel endow the hydrogel with excellent NIR photothermal responsiveness, which significantly promotes the release of DOX. Consequently, the CS/CuS/PDA hydrogel reveals excellent synergistically enhanced NIR, pH, and thermal triple-responsiveness, indicating its tremendous promise in the field of controlled drug release.
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高影响因子
Acta Biomaterialia
·
2016
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IF 6.008
Maohua Chen,Yun Zhang,Zhoujiang Chen,Songzhi Xie,Xiaoming Luo,Xiaohong Li
Challenges remain to load and deliver two or multiple drugs of complementary effects for synergistic cancer therapies. In the current study, multiarmed amphiphilic copolymers of 4-arm poly(ethylene glycol) (PEG) and polyaspartate (PAsp) are created for conjugation of camptothecin (CPT) and condensation with tumor necrosis factor-α (TNF) plasmids. Diethylenetriamine (DET) is grafted on PAsp, and CPT is conjugated onto PAsp(DET) by disulfide linkages to form hydrophobic cores of micelles , followed by condensation with TNF plasmids to form micelleplexes. The cis -aconitic linkers are introduced between PEG and PAsp(DET) to remove PEG shells in response to acidic pH, resulting in destabilized micelleplexes and prompted endosomal escape into the cytosol. The micelleplex disintegration in response to reductive stimuli in the cytosol leads to an efficient CPT release and pDNA disassociation. The co-delivery of CPT with TNF plasmids enhances the gene transfection of micelleplexes at low N/P ratios, and shows synergetic cytotoxicities to tumor cells with 2.5 and 8 folds lower IC 50 s compared with those after treatment with CPT or TNF alone, respectively. The micelleplex treatment on 4T1 tumor models dramatically extends the animal survival and suppresses the tumor growth with 2.3 and 3 folds lower in volume compared with CPT or TNF treatment alone, respectively. Histological and biochemical analyses display TNF expressions in tumor tissues after micelleplex treatment, resulting in significantly larger necrotic regions in tumors, higher cell apoptosis rates , and no obvious sign of tumor metastasis in lungs compared with other treatment. Therefore, the multifunctional micelleplexes based on multiarmed PEG-PAsp(DET) copolymers offer the targeted drug/gene delivery, dually responsive drug/gene release and synergistic antitumor efficacy, holding great promises for combination therapies. Statement of Significance Micelleplexes are constructed from multiarmed amphiphilic copolymers with conjugation of captothecin (CPT) and condensation of tumor necrosis factor-α (TNF) plasmid. The pH/redox stimuli realize co-delivery of CPT and pDNA in a sequential manner of folate-mediated endocytosis , endosomal escape induced by PEG cleavage, reduction-sensitive release of CPT in cytosol, and pDNA release from disintegrated polyplexes after CPT release. Compared with CPT or TNF treatment alone, the micelleplexes achieve 2.5 and 8 folds higher cytotoxicities to tumor cells, and suppress the tumor growth with 2.3 and 3 folds lower in volume, respectively. It demonstrates a feasible strategy to develop multifunctional micelleplexes with simultaneous drug conjugation and pDNA condensation, dually responsive drug/gene release and synergistic antitumor efficacy, holding great promise for combinational therapies.
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PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
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IF 10.723
Shumin Chen,Xiaoguo Ji,Mengyao Zhao,Jiayang Jin,Haiping Zhang,Liming Zhao
AZD9291 can prolong the survival of patients with non-small cell lung cancer. Unfortunately, resistance to AZD9291 is inevitable and hinders effectiveness. Studies showed the combination of Cyclosporin A (CsA) and AZD9291 could increase the efficacy of AZD9291, but the delivery efficiency of free drugs was limited. A chitooligosaccharide (COS) -based nanoparticle with enhanced delivery efficiency and endocytosis was constructed in this study. The results showed that this pH/redox cascade responsive nanoparticles improved therapeutic effect. The system is small and the surface charge changed from negative to positive according to the weakly acidic tumor microenvironment. After endocytosis, the nanoparticles decomposed and released AZD9291 and CsA in redox-rich cytoplasm. Experiments in vitro and in vivo proved that the nanoparticles overcame the biological barrier and significantly enhanced the anti-tumor effect of AZD9291. The novel multifunctional nanoparticle provides a way to overcome the drug resistance and the possibility of clinical application.
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PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
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IF 6.044
Ding Qu,Mengying Jiao,Haijiao Lin,Chunli Tian,Guowei Qu,Jingwei Xue,Lingjing Xue,Caoyun Ju,Can Zhang
Controlled release and tumor-selective distribution are highly desirable for anticancer nanomedicines. Here, we design and synthesize an anisamide-conjugated N -octyl- N,O -maleoyl- O -phosphoryl chitosan (a-OMPC) which can form amphiphilic micelles featuring pH-responsive release and high affinity to sigma-1 receptor-overexpressed tumors for paclitaxel (PTX) delivery. Thereinto, maleoyl and phosphoryl groups cooperatively contribute to pH-responsive drug release due to a conversion from hydrophile to hydrophobe in the acidic microenvironment of endo/lysosomes. We demonstrated that PTX-loaded a-OMPC micelles (PTX-aM) enhanced the cellular internalization via the affinity between anisamide and sigma-1 receptor, rapidly released drug in endo/lysosomes and elevated the cytotoxicity against PC-3 cells. The in vivo studies further verified that PTX-aM could largely accumulate at the tumor site even after 24 h of administration, resulting in obvious inhibition effect and prolonged survival period in PC-3 tumor xenograft-bearing mice. Moreover, OMPC showed no obvious hemolytic and acute toxicity. Collectively, this chitosan derivate holds a promising potential in application of prostate cancer-targeted drug delivery system.
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PubMed
高影响因子
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