KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2021
·Vol. 38
·期 5
·1066-1077
·
IF 3.309
Lu Yuan,Dai Hongqian,Cheng Pengpeng,Shi Hanbing,Tang Lan,Sun Xingyuan,Ou Zhimin
A modular approach was applied for the synthesis of bienzyme-polymer nanoconjugates (nano-BECs) (50–70 nm) consisting of two enzymes (carbonyl reductase and glucose dehydrogenase) conjugated within a single universal polymer scaffold. The amount of the product ethyl (R)-2-hydroxy-4-phenylbutyrate (R-HPBE) with nano-BECs as the catalyst was 533 mM in a dibutyl phthalate-phosphate buffer (dibutyl phthalate-PB) biphasic system, while the amount of R-HPBE was 349 mM using carbonyl reductase-poly(acrylic acid) as the catalyst, indicating that the nano-BECs have an advantage for coenzyme regeneration. Compared with a single aqueous phase, the substrate treatment capacity was improved at the interface of the dibutyl phthalate-PB biphasic system. Under the optimal reaction conditions (35 °C, 40 h, dibutyl phthalate-PB 1: 1), nano-BECs can completely convert substrate into optically pure R-HPBE (enantiomeric excess (e.e.) >99.9%) in the organic-aqueous system.
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ChemistrySelect
·
2023
·Vol. 8
·期 39
·e202302583
·
IF 2.1
Chunyan Yang,Qin Yang,Lei Chen,Nan Xu,Fubin Chen,Ting Pu
Graphical N-doped yellow fluorescent carbon quantum dots (Y-CDs) and red fluorescent carbon quantum dots were prepared by reacting o-phenylenediamine and p-phenylenediamine with terephthalic acid using solvothermal method. The emission wavelengths of Y-CDs and R-CDs are located at 530 nm and 600 nm, respectively. These two types of carbon dots can quench water in organic solvents and determine the content of water in them. In this work, we reported two nitrogen doped carbon quantum dot fluorescence sensors (Y-CDs and R-CDs) with good stability and luminescence efficiency, which can be quickly, sensitively and low-cost for the determination of trace water content in organic solvents. Using o-phenylenediamine and p-phenylenediamine as nitrogen sources and terephthalic acid as carbon sources, the preparation was carried out through simple solvothermal synthesis and heteroatom doping. The luminescence characteristics and interaction mechanism with the target were discussed. The results indicate that the two carbon dots can effectively quench the water content in acetone, ethanol, and DMF, as well as physical interactions such as hydrogen bonding and dynamic quenching, which are the reasons for the observed quenching effect. Based on this, a new method for determining water content in organic solvents using carbon quantum dot fluorescence probes has been established. The linear relationship between the fluorescence quenching efficiency F 0 /F of two carbon quantum dots and the water content in different organic solvents was studied. The detection limits (LOD) of water in acetone, ethanol and N,N-dimethylformamide (DMF) were 0.17 %, 2.35 %, 0.26 %, respectively, determined by Y-CDs. The LOQ of acetone, ethanol, and DMF were 0.554 %, 7.83 %, 0.855 %, respectively. The LOD of water in acetone, ethanol and DMF determined by R-CDs were 0.056 %, 0.69 %, 0.27 % and the LOQ was 0.187 %, 2.290 %, 0.909 %, respectively. Good recovery rates and precision were achieved in practical applications.
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FISH & SHELLFISH IMMUNOLOGY
·
2023
·
IF 4.7
Lili Yun,Meiru Kang,Yihao Shen,Junchang Feng,Guokun Yang,Jianxin Zhang,Xiaolin Meng,Xulu Chang
This study aimed to evaluate the effects of dietary supplementation with Bacillus velezensis R-71003 combined with sodium gluconate on antioxidant capacity, immune response and resistance against Aeromonas hydrophila in common carp . In addition, the biocontrol potential of the secondary metabolites of B. velezensis R-71003 was also evaluated to analyze the possible mechanism of B. velezensis R-71003 against A. hydrophila . The results indicated that the antibacterial crude extract of B. velezensis R-71003 can destroy the cell wall of A. hydrophila . Moreover, the results showed that dietary B. velezensis R-71003 could promote antioxidant capacity, which significantly increased the activities of CAT and SOD and decreased the content of MDA. Additionally, B. velezensis R-71003 supplementation significantly enhanced the immunity of common carp, as measured by the mRNA expression levels of cytokine-related genes ( TNF-α , TGF-β , IL-1β and IL-10 ). In addition, dietary B. velezensis R-71003 exhibited an upregulation of IL-10 and a downregulation of IL-1β, coupled with higher survival rates when challenged with A. hydrophila compared to the positive group. Furthermore, compared to prechallenge, the mRNA expression levels of TLR-4 , MyD88 , IRAK1 , TRAF6 , TRIF and NF-κB in the head kidney of common carp were significantly increased after challenge. The fish fed the B. velezensis R-71003 diet showed lower expression of TLR-4 , MyD88 , IRAK1 , TRAF6 , TRIF and NF-κB after the challenge than those fed the control diet. Thus, this study revealed that B. velezensis R-71003 can improve the resistance of common carp to pathogenic bacteria by destroying bacterial cell walls and improving fish immunity by activating the TLR4 signaling pathway . Importantly, this study indicated that sodium gluconate has a positive effect on B. velezensis R-71003 in enhancing the anti-infection ability of common carp. The results of this study will lay the foundation for the application of B. velezensis R-71003 in combination with sodium gluconate as an alternative to antibiotics in aquaculture.
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Journal of Pharmaceutical Analysis
·
2019
·
IF 4.44
Satar Tursynbolat,Yrysgul Bakytkarim,Jianzhi Huang,Lishi Wang
The simultaneous electrochemical determination of myricetin and rutin remains a challenge due to their indistinguishable potentials. To solve this problem, we constructed a ternary platinum nanoparticle, reduced graphene oxide, multi-walled carbon nanotubes (Pt@r-GO@MWCNTs) nanocomposite via a facile one-pot synthetic method. Under the optimized conditions, the ternary Pt@r-GO@MWCNTs nanocomposite exhibited good electrocatalytic activity toward myricetin and rutin via solid phase extraction and excellent performance for the simultaneous determination of myricetin and rutin. The oxidation peak current of myricetin was proportional to its concentrations in the range of 0.05–50 μM with a detection limit of 0.01 μM (S/N = 3). The linear range for rutin was 0.05–50 μM with a detection limit of 0.005 μM (S/N = 3). The ternary nanocomposite sensor also exhibited good reproducibility and stability, and was successfully used for the simultaneous determination of myricetin and rutin in real orange juice samples with recoveries ranging between 100.57% and 108.46%.
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CHIRALITY
·
2018
·Vol. 30
·期 6
·769-776
·
IF 1.833
Yilong Wang,Yongkai Xu,Yun Zhang,Aijun Sun,Yunfeng Hu
The two enantiomers of ethyl 3-hydroxybutyrate are important intermediates for the synthesis of a great variety of valuable chiral drugs. The preparation of chiral drug intermediates through kinetic resolution reactions catalyzed by esterases/lipases has been demonstrated to be an efficient and environmentally friendly method. We previously functionally characterized microbial esterase PHE21 and used PHE21 as a biocatalyst to generate optically pure ethyl ( S )-3-hydroxybutyrate. Herein, we also functionally characterized one novel salt-tolerant microbial esterase WDEst17 from the genome of Dactylosporangium aurantiacum subsp. Hamdenensis NRRL 18085. Esterase WDEst17 was further developed as an efficient biocatalyst to generate ( R )-3-hydroxybutyrate, an important chiral drug intermediate, with the enantiomeric excess being 99% and the conversion rate being 65.05%, respectively, after process optimization. Notably, the enantio-selectivity of esterase WDEst17 was opposite than that of esterase PHE21. The identification of esterases WDEst17 and PHE21 through genome mining of microorganisms provides useful biocatalysts for the preparation of valuable chiral drug intermediates.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2012
·Vol. 19
·期 5
·1385-1391
·
IF 2.651
Liang Bin,Yao Qian,Cheng Haoyi,Gao Shuhong,Kong Fanying,Cui Dan,Guo Yuqi,Ren Nanqi,Wang Aijie
Purpose With the aim of enhanced degradation of azo dye alizarin yellow R (AY) and further removal of the low-strength recalcitrant matter (LsRM) of the secondary effluent as much as possible, our research focused on the combination of aerobic bio-contact oxidation (ABO) with iron/carbon microelectrolysis (ICME) process. Materials and methods The combined ABO (with effective volume of 2.4 l) and ICME (with effectively volume of 0.4 l) process were studied with relatively short hydraulic retention time (HRT) of 4 or 6 h. Results At the HRT of 6 h with the reflux ratio of 1 and 2, the AY degradation efficiency in the final effluent was >96.5%, and the total organic carbon (TOC) removal efficiency were 69.86% and 79.44%, respectively. At the HRT of 4 h and the reflux ratio of 2, TOC removal efficiency and AY degradation efficiency were 73.94% and 94.89%, respectively. The ICME process obviously enhanced the total AY removal and the generated micromolecule acids and aldehydes then that wastewater backflow to the ABO where they were further biodegraded. Conclusion The present research might provide the potential options for the advanced treatment azo dyes wastewater with short HRT and acceptable running costs.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Ying Zhong,Xiao Chen,Shiyao Liu,Huibin Liu,Deng Hu,Gwendoline Lafaye,Changhai Liang
The industrial advancement of downstream products resulting from the directed hydrogenation of maleic anhydride is hindered by the limitations related to the activity and stability of catalysts. The development of nonprecious metal intermetallic compounds, in which active sites are adjustable in the local structures and electronic properties embedded within a distinct framework, holds immense potential in enhancing catalytic efficacy and stability. Herein, we report that nickel-based silicides catalysts, RNi2Si2 (R = Ca, La, and Y), afford high efficiency in the selective hydrogenation of maleic anhydride. Among the RNi2Si2 with the same tetragonal structure, CaNi2Si2 with complete isolation and highest electron density of Ni sites presents the low apparent activation energy (98.4 kJ/mol). Preferential adsorption of intermediate succinic anhydride via C═O bonds is achieved by the high oxygen affinity of CaNi2Si2, resulting in chemoselectivity to γ-butyrolactone (>80%). Additionally, the silicide with good resistance to sintering and acid corrosion presents remarkable stability for at least 130 h. The design of the silicide structure will offer fresh perspectives on the development of effective and enduring selective hydrogenation catalysts.
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JOURNAL OF ETHNOPHARMACOLOGY
·
2024
·
IF 4.8
Rong Ma,Yuge Zhou,Weifan Huang,Xiaoni Kong
Ethnopharmacological relevance Epimedium Tourn. ex L. is a traditional Chinese medicine used for thousands of years in China to treat forgetfulness. Icariin is a principal component of the genus Epimedium . Aim of the study The metabolic mechanism of icariin treating forgetfulness is explored. Materials and methods A D-galactose-induced senescent mouse model was employed. The cognitive performance of mice was assessed in the fear conditioning test. Hippocampal pathology was assessed in the immunohistochemistry assay. Plasma metabolome was analyzed using GC-MS method, and the differential metabolites were further identified by UPLC-MS/MS or GC-MS method. The liver function, including ALT and AST, was assessed by enzyme reaction. Icariin was administered intraperitoneally at 50 and 100 mg/kg. Mice were administered five consecutive days per week for 8 weeks. Results Icariin treatment improved hippocampus-related fear memory but not amygdala-related memory, whereas Pexidartinib (PLX3397), a microglial scavenger, did not. Icariin treatment maintained the TMEM119-positive microglial population and decreased the accumulation of the senescent biomarker p16 in the dorsal hippocampus in senescent mouse brains, whereas PLX3397 did not. Notably, p16 in the CA2 subregion significantly decreased in icariin-treated mice than the other hippocampal subregions. The senescent mice exhibited the circulating metabolic characteristics of mild ketoacidosis, active tricarboxylic acid (TCA) cycle, lactic acidosis, hyperglycemia, active detoxification, active cis-oleic acid metabolism, and inhibitory GABA shut. R-3-hydroxybutyric acid primarily produced in the liver was selectively and robustly decreased by icariin treatment, which was not observed with PLX3397. The TCA cycle was rescued in senescent mice by icariin treatment. Icariin also protected liver function (plasma ALT) in D-gal-induced senescent mice. Conclusions Icariin may protect mouse hippocampal cognition from D-gal-induced senescence by protecting microglial homeostasis, and facilitating the utilization of R-3-hydroxybutyric acid is one of the underpins.
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INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Chengshun Liu,Jianlong Li,Miao Wang,Guotai Jian,Chen Zhu,Hanxiang Li,Yongxia Jia,Jinchi Tang,Lanting Zeng
Camellia sinensis var. assamica is a major variety used to produce black tea. However, the core compounds influencing its aroma quality are unknown. In this study, Yinghong No. 9 black tea (YJBT), a distinctive black tea in South China, was analyzed. We detected 44 aroma compounds and identified seven key contributors, namely linalool, geraniol, trans -linalool oxide (furanoid), hexanal, methyl jasmonate, phenylacetaldehyde, and β-ionone. Linalool, which was generally more abundant in spring tea than in summer tea, contributed significantly to sweet and floral aromas. ( R )-Linalool was the primary linalool form in Yinghong No. 9, wherein it was highly abundant in high-quality tea leaves. The substantial expression of a linalool synthase gene and the relatively high catalytic efficiency of the encoded enzyme likely contributed to the accumulation of ( R )-linalool. This study identified the primary aroma compounds in YJBT, providing a foundation for advancing our knowledge of the aromatic quality of black tea.
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Journal of Energy Storage
·
2024
·
IF 8.9
Bairui Tao,Xiaoyan Feng,Fengjuan Miao
In order to solve the problem of glucose detection in medical development and meet the urgent demand for new energy storage devices, this paper proposes a new material suitable for glucose sensing and capacitive properties. The sensor was fabricated by depositing Co 3 O 4 nanoparticles onto nickel foam with integrated r-GO via the hydrothermal method, followed by the synthesis of CuO nanoparticles using electroplating. The detection range of the sensor is 0.3–11.3 mM. The sensor's sensitivity is 1000.3 μA mM −1 cm −2 , indicating its responsiveness to changes in analyte concentration. In electrochemical test systems, Signal-to-Noise Ratio (SNR) usually represents the relative intensity of the effective current signal and the background noise, reflecting the accuracy and reliability of the measurement. When the SNR is three, the minimum detection limit is 0.431 μM, highlighting its ability to reliably detect analytes at low concentrations amidst background noise. According to electrochemical workstation tests, the sensor demonstrates robust stability. Furthermore, the electrode material proves suitable for asymmetric supercapacitor devices, when the current density is 2 Ag −1 , the specific capacitance is 660.5 Fg −1 . At the same time, we also explore the cyclic stability of the device, which can retain 92.3 % of its initial specific capacitance after 5000 cycles, showing its remarkable long-term stability. In addition, the prepared nanocomposites can also light the red LED light. The results show that the synthesized CuO/Co 3 O 4 /r-GO/NF electrode can be used for electrochemical glucose sensing and supercapacitors, and plays an important role as a multi-functional material in the fields of medical, food, electronics, transportation and energy, providing key technical support for a variety of applications.
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ACS Omega
·
2020
·
IF 2.87
Xinming Feng,Jing Guo,Rubing Zhang,Wei Liu,Yujin Cao,Mo Xian,Huizhou Liu
An aminotransferase ω-TAEn was identified from Enhydrobacter aerosaccus. The ω-TAEn was successfully expressed in Escherichia coli and the obtained enzyme showed activity toward β-phenylalanine (β-phe) at optimal conditions. For optically pure (R)-β-phe, 50% yield was observed by kinetic resolution of racemic amino with pyruvate as the amino acceptor. To obtain (S)-β-phe, the lipase/ω-TAEn catalytic system was adopted. The ω-TAEn showed strict stereoselectivity to the amino donor. The formation of (S)-β-phe was observed using 3-aminobutyric acid as the amino donor, and (S)-β-phe was obtained by asymmetric synthesis with a yield of 82%.
研究领域:
生物化学
·
生物催化
·
酶学
·
化学生物学
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 13
·13602-13618
·
IF 2.914
Yue Huifang,Lu Pei,Su Wei,Xing Yi,Li Rui,Wang Jiaqing
To optimize the simultaneous removal of NO x and Hg 0 , a series of Cu a Ce b Zr c O 3 /γ-Al 2 O 3 catalysts prepared by the impregnation method were explored and their physical and chemical properties were analyzed by X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) analysis, X-ray photoelectron spectroscopy (XPS), NH 3 -temperature-programmed desorption (NH 3 -TPD), H 2 -temperature-programmed reduction (H 2 -TPR), in situ diffuse reflectance infrared Fourier transform spectroscopy (in situ DRIFT), and Fourier transform infrared spectroscopy (FT-IR). The results showed that 15% Cu 1.4 Ce 0.55 Zr 0.25 O 3 /γ-Al 2 O 3 resulted in the highest conversion efficiency for the simultaneous removal of NO x (93%) and Hg 0 (85%) at low temperatures (200 to 300 °C). Meanwhile, 15% Cu 1.4 Ce 0.55 Zr 0.25 O 3 /γ-Al 2 O 3 showed good stability and resistance to SO 2 and H 2 O, which is due to its low crystallinity, good textural performance, and strong redox ability. According to the TPD, TPR, and XPS results, the strong acidic character of 15% Cu 1.4 Ce 0.55 Zr 0.25 O 3 /γ-Al 2 O 3 promoted the removal of NO x and Hg 0 . The synergistic effect between CuO and CeO 2 in 15% Cu 1.4 Ce 0.55 Zr 0.25 O 3 /γ-Al 2 O 3 can increase the mobility of chemically adsorbed oxygen and activates lattice oxygen, leading to an excellent performance. The DRIFT results showed that NH 3 , NH 4 + , nitrate, and nitrite participated in the selective catalytic reduction (SCR) reaction. On the basis of our experimental results, Hg 0 and NO x removal mechanisms were proposed as Hg (ad) + O* → HgO (ad) and 2NH 3 /NH 4 + (ad) + NO 2 /NO 3 − (ad) + NO→2N 2 + 3H 2 O/2H + , respectively.
研究领域:
环境催化
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大气污染控制
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材料化学
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表面科学
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工业生态学
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