SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Mengjie Su,Haitian Lan,Liang Tian,Mengyuan Jiang,Xiaoqing Cao,Cailing Zhu,Chunmei Yu
Levels of serotonin above or below normal values in the human body can cause a variety of diseases, including depression, Alzheimer's disease , and irritable bowel syndrome. In this study, we developed a novel electrochemical sensor based on Ti 3 C 2 T x -reduced oxide graphene (Ti 3 C 2 T x -rGO) nanocomposite modified glassy carbon electrode for the detection of serotonin in human biofluids. The Ti 3 C 2 T x -rGO nanocomposite was synthesized by hydrazine reduction followed by self-assembly, which was characterized using scanning electron microscopy, transmission electron microscopy coupled energy-dispersive X-ray analysis, and Raman spectroscopy. The obtained Ti 3 C 2 T x -rGO nanocomposite could not only mitigate the restacking problem of Ti 3 C 2 T x nanosheets but also provide a large surface area and effective active sites for improving the electrochemical performance of the nanocomposite. The electrochemical sensor fabricated based on the Ti 3 C 2 T x -rGO nanocomposite showed high sensitivity, excellent specificity, and good stability for serotonin detection. Additionally, it was successfully used to determine serotonin in human blood plasma, and the results were within the expected range. The serotonin detection method demonstrated in this work is expected to be applied for the prevention and rapid diagnosis of serotonin-related diseases.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Linlin Zhang,Jie Wang,Chenglong Zhao,Feijin Zhou,Cheng Yao,Chan Song
The nanozymes-based sensing platforms have been attracted in recent years due to their outstanding performances in the practical applications. In this work, the magnetic Fe nanoparticles (NPs) with peroxidase-like activity was developed for the fast detection of glutathione (GSH). The Fe NPs were facilely prepared through the reduction of iron trichlorrde hexahydrate (FeCl 3 ‧6H 2 O) by sodium borohydride (NaBH 4 ) at room temperature , exhibiting a merit of simplicity. Significantly, the achieved Fe NPs have high peroxidase-like catalytic activity . At the room temperature, the colorimetric substrates such as 3,3′,5,5′-tetramethylbenzidine (TMB) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) can be rapidly oxidized by Fe NPs with the help of H 2 O 2 . After incubated for just 30 s, the catalytic system could achieve a colored solution. Thus, the Fe NPs-based sensing systems can be employed in the development of the colorimetric biosensors with the naked-eye detection.
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BIOSENSORS & BIOELECTRONICS
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2022
·
IF 12.545
Weisu Kong,Da Zhu,Rengan Luo,Siqi Yu,Huangxian Ju
Traditional photoelectrochemical (PEC) systems with inorganic semiconductors as photoactive materials generally involve effortless recombination of electron-hole pairs, which greatly limit the detection sensitivity. The arrangement of multiple components with tunable bandgaps provides an effective way to accelerate charge transfer. In this work, a framework material with adjustable structure was used to promote the charge transfer in the PEC process. The framework was constructed with 9,10-di(p-carboxyphenyl)anthracene (DPA) ligands as the light collector to coordinate with Zn 2+ nodes, which formed an electronegative metal-organic framework (ZnMOF), and showed good conductivity and PEC performance due to the π-π stacking of DPA and the intrareticular charge transfer. Based on the band and charge matching of dopamine (DA) with ZnMOF, the ZnMOF modified electrode as a biosensor showed excellent PEC response to DA with good selectivity, thus realized sensitive detection of DA ranging from 0.03 to 10 μM with a detection limit of 17.7 nM. The biosensor could be used to monitor the release of DA from PC12 cells and evaluate the stimulation of K + to DA release . The conductive framework material provided an approach to develop highly selective sensing platform for trace bioanalysis .
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ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Haoye Wang,Aijuan Xie,Shuji Li,Jiajun Wang,Kaixuan Chen,Zilong Su,Ningning Song,Shiping Luo
A three-dimensional g-C 3 N 4 /MWNTs/GO hybrid modified electrode was constructed as an electrochemical sensor for the simultaneous determination of ascorbic acid (AA), dopamine (DA) and uric acid (UA). Due to the high conductivity of MWCNTs and the strong synergy between g-C 3 N 4 and GO , the combination of the three effectively improved the electrocatalytic activity of the modified electrode for the oxidation of AA, DA, and UA, and solved the problems such as overlapping anodic peaks. The electrochemical performance of the as-constructed sensor was investigated and optimized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The linear response range of AA, DA, and UA in the optimal condition was 0.2–7.5 mM, 2–100 μM, and 4–200 μM, respectively. The detection limits (S/N = 3) of AA, DA, and UA were 96, 0.22, and 1.36 μM, respectively. The recoveries of AA, DA and UA in serum samples from three groups were 92.82–106.50%, and the relative standard deviations were less than 2%. The results show that the as-constructed g-C 3 N 4 /MWNTs/GO modified electrode has the advantages of simplicity, high sensitivity and good selectivity , and can simultaneously determine AA, DA, and UA.
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ChemElectroChem
·
2022
·Vol. 9
·期 10
·e202200237
·
IF 4.782
Xiaoxiao Chen,Pu Li,Chen Luo,Chaobiao Huang
Graphical Detection mechanism of PEC sensor for detecting xanthine based on photocurrent signal changes caused by hydrogen peroxide reduction catalyzed by copper nanoclusters A photoelectrochemical (PEC) sensor combining chitosan-coated and glutathione-protected copper nanocluster (CS−GSH−CuNCs) with peroxidase-like activity and xanthine oxidase (XAO) was developed for rapid detection of xanthine. Firstly, CS−GSH−CuNCs was prepared by glutathione (GSH) reduction using chitosan (CS) as protective agent, and then was modified on titanium dioxide nanoparticles (TiO 2 NPs)/ITO electrode which was prepared by sintering titanium dioxide (TiO 2 ) on the ITO electrode surface by high temperature calcination. After excited by light at 350 nm, the photoinduced electrons from the lowest unoccupied molecular orbital (LUMO) of CS−GSH−CuNCs were transferred to the conduction band of TiO 2 , which resulted in spatial separation of electron-hole and enhancement of photocurrent signal. XAO oxidizes xanthine to produce hydrogen peroxide (H 2 O 2 ). Part of the photoinduced electrons from CS−GSH−CuNCs transferred and catalyzed the reduction of H 2 O 2 , resulting in the reduction of the photocurrent. Under optimal conditions, the PEC sensor exhibits good sensitivity and reproducibility with the limit of detection (LOD) of 6.6 nmol ⋅ L −1 and a relative standard deviation of 3.5 % for 10 replicate detections of 1.00 μmol ⋅ L −1 xanthine and the linear range of 0.04–90.0 μmol ⋅ L −1 for xanthine. Furthermore, the PEC sensor presents nice selectivity owing to its enzyme-like activity and was successfully applied to human urine, indicating its great potential for real sample analysis.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2022
·
IF 4.598
Mingzhu Cui,Zhiqiang Xie,Meiling Wang,Xianming Zhang
Development of a high-performance electrode materials for the simultaneous sensing of ascorbic acid (AA), dopamine (DA) and uric acid (UA) is critical in clinical diagnostics. Novel polymer hydrogel-derived porous carbon has been investigated in sensing due to their high conductivity, hierarchical porous structure and environmental benignity. However, the high cost and cumbersome synthesis of the crosslinker for polymer hydrogel precursors make carbon production by pyrolysis greatly compromised. To address this challenge, here, a facile approach to N, O, S co-doped three-dimensional porous carbon frameworks (N, O, S-HCF) derived from methyl orange (MO) crosslinked polypyrrole hydrogel is developed. Experimental and spectroscopy demonstrate that the derived 3D porous structure and heteroatom doping effect create multidimensional electron transfer pathways and electrocatalytic activity centers. Specifically, the sensor exhibits potent and persistent electrochemical behavior with well-separated oxidation peaks and enhanced oxidation peak currents towards ascorbic acid (AA), dopamine (DA) and uric acid (UA). As a result, the N, O, S-HCF contributes to the effective determination of the three analytes in their mixture and the calibration curves show linear range of 2.5 × 10 −6 –1.0 × 10 −2 M, 1.8 × 10 −7 –8.7 × 10 −5 M, and 2.5 × 10 −7 –1.0 × 10 −4 M with detection limits of 1.0 × 10 −6 , 1.2 × 10 −7 , and 1.8 × 10 −7 M for AA, DA and UA, respectively. This study provides a simple strategy to build heteroatom doped porous carbon for the simultaneous monitoring of multiple electroactive species.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2022
·
IF 5.876
Zhiqiang Zhou,Yuanping Liao,Huiyan Wu,Qi Xiao,Yi Liu,Liyun Yang
Herein, zeolitic imidazolate framework-8 capped gold nanoclusters (AuNCs@ZIF-8) was fabricated and developed as an “on-off-on” fluorescent nanoprobe for selectively and sensitively detecting KMnO 4 and ascorbic acid (AA). The prepared AuNCs@ZIF-8 initially exhibited strong fluorescence at 595 nm KMnO 4 could quench the fluorescence of AuNCs@ZIF-8, rendering the fluorescence signal of AuNCs@ZIF-8 in the “off” state. The probe allows KMnO 4 to be detected with a 10 nM detection limit and the linear analytical range was 0.05–2 μM. When AA was added into the AuNCs@ZIF-8/KMnO 4 system, the fluorescence of the AuNCs@ZIF-8 recovered due to the redox reaction between KMnO 4 and AA, and the fluorescence signal of the system turned “on”. The detection limit of AA was 37 nM in a good linear concentration range of 0.05–5 μM. Furthermore, this AuNCs@ZIF-8 nanoprobe was successfully used for AA detecting in fresh orange juices with the recoveries ranged from 96.6% to 108.2%, which validates the potential applicability of this strategy.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Jiaying Zhao,Congjuan He,Weixuan Wu,Huisi Yang,Lan Peng,Li Wen,Zhikun Hu,Changjun Hou,Danqun Huo
Flexible sensing technology has received widespread attention in the monitoring of human physiological indicators. Thus, it is of great significance to develop a universal flexible sensing interface for early diagnosis of multi-level biomarkers. Herein, we reported a multifunctional 3D carbon fiber paper (CFP)-MXene-MoS 2 (CMM) sensing interface, which provided a flexible electrochemical biosensing platform for the ultrasensitive detection of multiple bioanalysis in biofluids. Notably, the CMM electrochemical interface was comprised of staggered carbon fibers on which the organ-like MXene-MoS 2 heterostructure covered with MoS 2 nanosheet arrays was uniformly allocated, permitting local enrichment of biomolecules for enhanced biomolecular capture efficiency, laying a solid foundation for the ultrasensitive detection of multiple biomolecules. In proof-of-concept experiments, we validated that the flexible CMM interface could be applied for ultrasensitive analyzing of quite a few biomolecules like ascorbic acid (AA), uric acid (UA), dopamine (DA) and microRNA (miRNA), with charming sensitivity and reproducibility. It was worth mentioned that an ultra-high sensitivity of 3.16 aM was acquired towards detecting microRNA. Under optimal parameters, the designed electrochemical interface worked well in complex biofluids such as human serum and urine samples. Overall, this flexible and disposable CMM interface with ultrahigh sensitivity provided insights for a more portable, accessible and valuable approach for monitoring biomolecules in clinical diagnosis.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Hangqing Xie,Zi Chen,Xiaoqin Wei,Qing Han,Haotuo Wang,Xiaolei Yang,Shiqing Xu,Xiang Wang
The fluoride ion (F – ) concentration in serum is an important indicator of fluorosis. However, the biofluorescence of serum itself makes the concentration of F – difficult to detect. Herein, a Tb‐1@F127 nanoprobe is synthesized by Flash NanoPrecipitation of Tb 3+ , Ga 3+ , organic ligands and block copolymer Pluronic (F127). The absorbance and photoluminescence (PL) of the Tb‐1@F127 nanoprobe are enhanced by the "antenna" effect of the organic ligands and the energy transfer of triplet excitons . The molar extinction coefficient of Tb‐1@F127 nanoprobe is 28,000 times higher than the f-f transfer in free Tb 3+ . Moreover, the addition of F – blocks the above energy transfer process , resulting in a decrease in the PL intensity of Tb‐1@F127 nanoprobe. In the serum solution, the PL intensity of Tb‐1@F127 nanoprobe has a linear response to the concentration of F – in the range of 0–18.81 ppm, and the detection limit is 0.056 ppm. The Tb‐1@F127 nanoprobe demonstrates high sensitivity and selectivity for the detection of F concentration, and is not affected by biological autofluorescence.
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NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 20
·9595-9601
·
IF 3.925
Hongbo Wu,Yingjie Xu,Ruyan Xie,Mingyu Tang,Lihua Chen,Fengyu Qu,Changhong Guo,Fang Chai
Facile and accurate detection of dopamine (DA) in biological samples is crucial for diagnostic needs. This study reports a portable colorimetric fluorescent probe for the tracing of DA based on orange-red emitting Au nanoclusters (AuNCs). The AuNCs were reduced and stabilized with L-methionine by strictly controlling the reaction conditions. The resultant L-methionine capped AuNCs (M-AuNCs) exhibit bright and stable orange-red emission with a quantum yield of 31.08%. The excitation/emission wavelength is located at 420 nm/610 nm, and the large Stoke's shift of 190 nm endows the M-AuNCs with great potential as a late-model sensing probe for life studies and environmental science. The M-AuNCs exhibit unique specificity and excellent sensitivity for tracing DA by an “on–off” strategy with a lower limit of detection (LOD) of 3.96 nM (S/N = 3) within a linear range of 10–100 nM. Notably, the colorimetric detection results can be explored as a visual sensor for sensitive, reliable and real-time/on-site detection of DA with the assistance of a smartphone. The LOD can be determined as 81.94 nM within a wide linear range of 0.1–1.0 μM by a smartphone. Furthermore, the M-AuNCs as a late-model potable probe allow simple and rapid discrimination of DA in serum, and the cellular imaging of the M-AuNCs indicated that the detection of DA can also be achieved in living cells, demonstrating their great feasibility for the tracing of DA in real samples.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Jilong Li,Yanhui Wang,Jianbing Zang,Yingke Zhou,Shengyao Su,Qi Zou,Yungang Yuan
Levels of dopamine (DA) in the human body is directly related to an individual’s health status. It is crucial to detect DA via economical and effective means, especially when it coexists with other molecules. A self-supporting array-like electrode composed of micron-diamond particles distributed in amorphous carbon (AC) matrix (D/AC) was synthesized by vacuum annealing the mixture of phenolic resin (PR) and micro-diamond. During the annealing process, the PR was converted to AC. The AC was divided into micron-level areas using micron-diamond particles to obtain an array-like electrode, which improved the response of electroanalysis signals. The D/AC exhibited a low limit of detection for DA (0.238 μM), which was attributed to the array-like structure. Moreover, outstanding selectivity towards DA on D/AC in the presence of uric acid was confirmed, and the evident peak potential differences (300 mV) facilitated detection of DA. The detection limit of DA on D/AC in the presence of uric acid was 0.334 μM. Accordingly, D/AC may be suitable as biosensor candidate due to its decent stability, reproducibility and selectivity.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 7
·3799
·
IF 6.208
Lulu Xiong,Yuanyuan Zhang,Shiming Wu,Feng Chen,Lingli Lei,Ling Yu,Changming Li
A facile and ingenious method to chemical etching-coordinating a metal-organic framework (MOF) followed by an annealing treatment was proposed to prepare Co3O4nanoparticles uniformly dispersed in rational porous carbon nano-boxes (Co3O4@CNBs), which was further used to detect H2O2released from living cells. The Co3O4@CNBs H2O2sensor delivers much higher sensitivity than non-etching/coordinating Co3O4, offering a limit of detection of 2.32 nM. The wide working range covers 10 nM-359 μM H2O2, while possessing good selectivity and excellent reproducibility. Moreover, this biosensor was used to successfully real-time detect H2O2released from living cells, including both healthy and tumor cells. The excellent performance holds great promise for Co3O4@CNBs’s applications in electrochemical biomimetic sensing, particularly real-time monitor H2O2released from living cells.Keywords:nano-boxes;tannic acid;Co3O4@CNBs;hydrogen peroxide sensor;electrochemical detection;living cell
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