MOLECULES
·
2023
·Vol. 28
·期 16
·6064
·
IF 4.6
Shuai Xu,Shiyue Zhang,Yutong Li,Jiyang Liu
Visual detection based on nanozymes has great potential for the rapid detection of metabolites in clinical analysis or home-based health management. In this work, iron and nitrogen co-doped carbon dots (Fe,N-CDs) were conveniently synthesized as a nanozyme for the visual detection of glucose (Glu) or cholesterol (Chol). Using inexpensive and readily available precursors, Fe,N-CDs with peroxidase-like activity were conveniently prepared through a simple hydrothermal method. Co-doping of Fe and N atoms enhanced the catalytic activity of the nanozyme. The nanozyme had a low Michaelis constant (Km) of 0.23 mM when hydrogen peroxide (H2O2) was used as the substrate. Free radical trapping experiments revealed that the reactive oxygen species (ROS) generated in the nanozyme-catalyzed process were superoxide anion radicals (•O2−), which can oxidize colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to generate blue oxidation product (ox-TMB) with characteristics absorbance at 652 nm. Based on this mechanism, a colorimetric sensor was constructed to detect H2O2ranging from 0.1 μM to 200 μM with a detection limit (DL) of 75 nM. In the presence of glucose oxidase (Gox) or Chol oxidase (Chox), Glu or Chol was oxidized, respectively, and generated H2O2. Based on this, indirect detection of Glu and Chol was realized with linear detection ranges of 5–160 μM and 2–200 μM and DLs of 2.8 μM and 0.8 μM, respectively. A paper-based visual detection platform was fabricated using Fe,N-CDs as nanozyme ink to prepare testing paper by inkjet printing. Using a smartphone to record the RGB values of the testing paper after the reaction, visual detection of Glu and Chol can be achieved with linear detection ranges of 5–160 μM (DL of 3.3 μM) and 2–200 μM (DL of 1.0 μM), respectively.Keywords:visual detection;colorimetric sensor;nanozyme;carbon dots;metabolite detection
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INORGANIC CHEMISTRY
·
2023
·
IF 4.6
Yunfei Song,Chengzhi Huang,Yuanfang Li
Vacancy engineering as an effective strategy has been widely employed to regulate the enzyme–mimic activity of nanomaterials by adjusting the surface, electronic structure, and creating more active sites. Herein, we purposed a facile and simple method to acquire transition metal manganese oxide rich in oxygen vacancies (OVs-Mn2O3-400) by pyrolyzing the precursor of the Mn(II)-based metal–organic gel directly. The as-prepared OVs-Mn2O3-400 exhibited superior oxidase-like activity as oxygen vacancies participated in the generation of O2•–. Besides, steady state kinetic constant (Km) and catalytic kinetic constant (Ea) suggested that OVs-Mn2O3-400 had a stronger affinity toward 3,3′,5,5′-tetramethylbenzidine and possessed prominent catalytic performance. By taking 2-phospho-l-ascorbic acid as the substrate, which can be converted into reducing substance ascorbic acid in the presence of alkaline phosphatase (ALP), OVs-Mn2O3-400 can be applied as an efficient nanozyme for ALP colorimetric analysis without the help of destructive H2O2. The colorimetric sensor established by OVs-Mn2O3-400 for ALP detection showed a good linearity from 0.1 to 12 U/L and a lower limit of detection of 0.054 U/L. Our work paves the way for designing enhanced enzyme-like activity nanozymes, which is of significance in biosensing.
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JOURNAL OF FOOD BIOCHEMISTRY
·
2023
·
IF 4
Qiao Tong,Jianjun Xi,Yu Cao,Ruoyu He,Yanmei Zhao,Yidan Shao,Xuwang Pan,Jinsong Huang,Yongping Chen,Rangxiao Zhuang
Chronic fructose consumption is becoming one leading risk factor for NAFLD due to its hazard on cellular lipid metabolism, which plays an essential role in the pathogenesis of NAFLD. Morroniside, an iridoid glycoside from Cornus officinalis, has been suggested to have potent in regulating cellular glucolipid metabolism. However, the effect and mechanism of morroniside on improving fructose-induced hepatic steatosis and retarding NAFLD progression remain ambiguous. In this study, we illustrate the efficacy of morroniside in alleviating fructose-triggered hepatic steatosis in mice. Mechanically, morroniside suppressed de novo lipogenesis and promoted β-oxidation by inhibiting the activation of PGC1β. Additionally, morroniside was found to work in attenuating hepatic inflammation in response to long-term fructose intake. Taken together, the current study reveals that morroniside is a promising food and medicinal therapy for NAFLD treatment and is effective in delaying the progression of NAFLD to NASH, especially in subtypes caused by excessive carbohydrate intake.
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RENEWABLE ENERGY
·
2023
·
IF 8.7
Ronghua Zhang,Wenhao Zhang,Jianchun Jiang,Junming Xu,Kui Wang,Junfeng Feng,Hui Pan
Methyl levulinate (ML) and levulinic acid (LA), important platform chemicals derived from biomass carbohydrates, are potential candidates for use as fuel additives and chemicals. In this work, bifunctional solid acid catalysts were prepared by impregnation via an environmentally friendly method and used for the conversion of glucose to ML and LA. In the conversion of glucose to levulinate, the isomeric conversion of glucose into fructose mainly utilized the Lewis acid sites properties exhibited by chromium, and the dehydration of fructose to 5-hydroxymethylfurfural and its degradation to levulinate mainly exploited the Brønsted acid sites properties exhibited by A15 . The yield of ML and LA was 43.1 % and 1.2 %, respectively, and glucose was completely transformed into methanol within 2.0 h at 200 °C. The excellent activity of the best catalyst may be due to the sufficient total acidity and suitable ratio of the number of Lewis acid sites to that of Brønsted acid sites. The results for five catalysis cycles proved that the catalyst has good stability and reusability . In addition, the catalyst prepared was effective for the conversion of other carbohydrates, namely, sucrose, cellobiose, and 5-hydroxymethylfurfural to ML with yields of 83.3 %, 77.7 %, and 57.4 %, respectively.
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INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Shaochao Sun,Junqiang Zhang,Min Hong,Jialong Wen,Jiliang Ma,Runcang Sun
The utilization of photocatalysis for the simultaneous production of valuable fine chemicals and fuels from biomass-derived feedstocks holds significant promise; however, its practical implementation remains constrained. In this study, we present the development of an Fe-doped gallium nitride (GaN) catalyst treated by calcination (Fe@GaN-X) for the selectively conversion of biomass-based monosaccharides and xylan into lactic acid and carbon monoxide (CO). Fe@GaN-400 exhibited broader visible light absorption, lower resistance, and reduced photoluminescence intensity in comparison to pristine GaN, thereby affording exceptional photocatalytic activity (lactic acid yield: 71.38%; CO evolution rate: 385.74 μmol g −1 h −1 ). When monosaccharides and xylan were used as substrates, the Fe@GaN-400 system demonstrated outstanding photocatalytic activity, particularly in the case of xylan system (CO evolution rate = 923.21 μmol g −1 h −1 ). Additionally, the experimental results revealed the generation of distinctive reactive species, encompassing holes (h + ), superoxide anion (·O 2 - ), hydroxyl radical (·OH) and singlet oxygen ( 1 O 2 ) within this catalytic system. Remarkably, the ·O 2 - and h + exhibit heightened selectivity towards CO and lactic acid, respectively. This work establishes a novel pathway for the co-production of fine chemicals and fuels through the photocatalytic conversion of biomass.
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Food Bioscience
·
2023
·
IF 5.2
Shijin Xiong,Dandan Qiu,Xiaoyan Xu,Tonghao Du,Zhen Peng,Muyan Xiao,Tao Huang,Jinqing Huang,Hongbing Ren,Mingyong Xie,Tao Xiong
Laotan Suancai is a well-known Chinese traditional fermented vegetable with a unique flavor. However, there are significant differences in the quality of Laotan Suancai pickled by jars (LTSC-J) and pools (LTSC-P) on an industrial scale. To reveal the effect of fermentation craft on Laotan Suancai quality, the microbial community, flavor metabolites, and physicochemical properties of the two types of Laotan Suancai, as well as their interrelationship were investigated. LTSC-P had higher salinity, glucose content, and histidine content, while LTSC-J had higher TA, lactic acid content, and isobutyric acid content. Nine differential aroma-active compounds were found between the two types of Laotan Suancai: anethole , eucalyptol, diallyl sulfide, estragole, diallyl disulfide , eugenol, linalool, nonanal , and ethyl butyrate. LTSC-P showed a higher relative abundance of Weissella , Acinetobacter , Pseudomonas , Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium , Inhella , and Leuconostoc compared to LTSC-J. Co-occurrence analysis indicated the distinct differences in the co-occurrence patterns of the microbial network across the two types of Laotan Suancai. RDA analysis indicated that glucose, salinity, TA, maltose , and nitrite were the main drivers for the differences in bacterial communities between the two types of Laotan Suancai. Spearman correlation analysis revealed strong correlations between 18 bacteria and 61 flavor compounds. Finally, bacterial metabolic function prediction indicated that sulfide and aromatic ester may be biosynthesized by sulfur metabolism , cysteine and methionine metabolism, pyruvate metabolism, and butanoate metabolism. Overall, these findings provide important guidance for improving the manufacturing and quality of Laotan Suancai.
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Chemosensors
·
2023
·Vol. 11
·期 7
·398
·
IF 4.2
Pengjuan Ni,Siyuan Liu,Yizhong Lu
The threat of glyphosate to food safety has attracted widespread attention. Consequently, it is highly urgent to develop a sensitive and accurate method for glyphosate detection. Herein, a turn-on fluorescent method for glyphosate detection using polydopamine-polyethyleneimine (PDA-PEI) copolymer as a fluorescent probe andp-nitrophenylphosphate (PNPP)/alkaline phosphatase (ALP) as a fluorescence quenching system is developed. The PDA-PEI copolymer was prepared by a one-pot method under mild condition, and its fluorescence kept almost unchanged after storing in a refrigerator for one month. ALP catalyzed the hydrolysis of PNPP top-nitrophenol (PNP) that caused the fluorescence quenching of PDA-PEI copolymer via the inner filter effect. However, glyphosate inhibited ALP activity, thereby preventing the formation of PNP and restoring the fluorescence signal. Under the optimized conditions, the fluorescence of PDA-PEI copolymer depended on glyphosate concentrations ranging from 0.2 to 10 μg/mL with a detection limit of 0.06 μg/mL. Moreover, this assay was applied to detect glyphosate in real samples using the standard addition method. The recoveries were in the range from 88.8% to 107.0% with RSD less than 7.78%. This study provides a novel insight for glyphosate detection and expands the applications of fluorescent copolymer.Keywords:fluorescence;alkaline phosphatase;glyphosate;inner filter effect
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BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Hossain M. Zabed,Suely Akter,Mudasir A. Dar,Jamila A. Tuly,Mukesh Kumar Aswathi,Junhua Yun,Jia Li,Xianghui Qi
This study aimed to produce enhanced fermentable sugars from a novel stover system through the bioprocessing of its soluble sugars and insoluble carbohydrates. The pretreatment conditions were optimized for this high sugar-containing stover (HSS) to control inhibitor formation and obtain enhanced fermentable sugar concentrations. The optimum temperature, acid loading, and reaction time for the pretreatment were 155 °C, 0.5%, and 30 min, respectively, providing up to 97.15% sugar yield and 76.51 g/L total sugars at 10% solid-load. Sugar concentration further increased to 126.9 g/L at 20% solid-load, generating 3.89 g/L acetate, 0.92 g/L 5-hydroxymethyl furfural, 0.82 g/L furfural, and 3.75 g/L total phenolics as inhibitors. To determine the effects of soluble sugars in HSS on fermentable sugar yield and inhibitor formation, sugar-removed HSS was further studied under the optimum conditions. Although prior removal of sugars exhibited a reduction in inhibitor generation, it also decreased total fermentable sugar production to 115.45 g/L.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·Vol. 103
·期 15
·7631-7642
·
IF 4.1
Dongshuang Liang,Haosong Wen,Yaxuan Zhou,Taohong Wang,Guangqun Jia,Zongyan Cui,Adan Li
Background Honey aroma is one of its most important properties and it depends on the qualitative and quantitative composition of the volatile compounds. The volatile profile of honey could reveal its botanical origin to avoid a false characterization. Thus, it is of great significance to honey authentication. This study developed and validated a headspace solid-phase microextraction and gas chromatography–mass spectrometry (HS-SPME-GC–MS) method for simultaneous qualitative and quantitative analyses of 34 volatile components in honey. The developed method was applied to 86 honey samples from six different botanical origins, including linden honey, rape honey, jujube honey, vitex honey, lavender honey and acacia honey. Results The volatile fingerprints and quantitative results were simultaneously obtained by using the full scan and selected ion monitoring (SCAN+SIM) MS scanning mode. The limits of quantification (LOQs) and limits of detection (LODs) of 34 volatile compounds were in the ranges of 1–10 ng/g and 0.3–3 ng/g, respectively. And the spiked recoveries ranged between 70.6% and 126.2%, with the relative standard deviations (RSDs) not higher than 45.4%. A total of 98 volatile compounds were found with relative contents determined, and the 34 volatile compounds were determined with absolute concentrations. Based on the volatile fingerprints and the contents of volatile compounds, honey samples from six botanical origins were well classified by principal component analysis and orthogonal partial least-squares discrimination analysis. Conclusions The HS-SPME-GC–MS method was successfully applied to achieve the volatile fingerprints of six types of honey and to quantitatively analyze 34 volatile compounds with satisfying sensitivity and accuracy. Chemometrics analysis showed significant correlations between honey types and volatiles. These results reveal the characteristics of volatile compounds in six types of unifloral honey and provide some supports for honey authentication. © 2023 Society of Chemical Industry.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Yujia He,Rui Zhang,Weipeng Song,Huai Liu,Junhua Zhang,Wenlong Jia,Lincai Peng
The simultaneous valorization of biomass-based C5 and C6 sugars to furfural, a versatile intermediate, is of great significance to fully utilize biomass components. Inability to effectively transform C6 sugars remains a major barrier toward this challenging goal. We herein report the 1,4-dioxane-mediated solvent system with Hβ zeolite catalyst for the transformation of glucose giving high yield to furfural (57%). The maintenance of intrinsic balanced Lewis and Brønsted acid sites from Hβ zeolite that is credited to the positive solvent effect of 1,4-dioxane is highly conductive to the selective C-C bond cleavage of glucose. Also importantly, the introduction of proper amount of H 2 O into the reaction system results in a high formation rate and an improved selectivity toward furfural due to the induction of highly reactive acyclic glucose. Glucose-to-furfural through 3-deoxyglucose should be more favorable than that via fructose. Impressively, the developed protocol can also be adapted to C5 sugars dehydration, and the one-pot conversion of glucose and xylose mixtures gains a furfural yield as high as 63%. The activation energy barrier for furfural production is higher from glucose compared to xylose. This work not only develops a promising protocol for furfural production simultaneously from C5 and C6 sugars but also provides insights into the mechanistic role of the solvent 1,4-dioxane to availably manipulate the acid-catalyzed conversion of C6 sugars.
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ACS Sustainable Chemistry & Engineering
·
2023
·
IF 8.4
Guoxing Liu,Huai Liu,Junhua Zhang,Rui Zhang,Heng Zhang,Yong Sun,Lincai Peng
Catalytic conversion of concentrated carbohydrates and raw biomass into 5-hydroxymethylfurfural (HMF), an important and value-added platform chemical, is highly desirable for industrial applications but challenging. In this work, the tin pyrophosphate-oxide (SnP2O7–SnO2) catalyst has been designed to efficiently produce HMF from various concentrated carbohydrates and raw biomass. Especially, a high HMF yield of 67.8% was implemented over the SPC4 catalyst from concentrated glucose (36 wt %) in a biphasic solvent system of water and methyl isobutyl ketone. Moreover, the SPC4 catalyst disclosed a wide range of universality for converting various concentrated carbohydrates and raw biomass to HMF with satisfactory yields (45.9–79.5%). To the best of our knowledge, this is the first example of accomplishing appreciable HMF yields from carbohydrates and raw biomass at such a high substrate concentration. It has been demonstrated that the superior catalytic performance of the catalyst should originate from its balanced Lewis and Brønsted acidity and superhydrophilicity property. This work invents a highly promising heterogeneous catalyst for efficiently valorizing renewable lignocellulose-related compounds into versatile furans.
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Catalysts
·
2023
·Vol. 13
·期 6
·969
·
IF 3.9
Srinivasan Vinju Vasudevan,Jin Cai,Junming Xu,Hongjian Lin,Hongliang Wang,Quan Bu
In this work, the microwave-assisted reaction of 5-hydroxymethylfurfural (5-HMF) into valuable ether and acylated production formation was investigated with the help of molybdophosphoric acid encapsulated dendritic fibrous silica (KCC-1) as a catalyst. XRD, N2adsorption-desorption, SEM, FT-IR, NH3-TPD, and TEM were used to analyze the physicochemical and structural properties of the synthesized catalysts. The microwave etherification of 5-HMF with ethanol was tested using synthesized catalysts. The effects of the reaction temperature, reaction time, catalytic amount, and microwave power were investigated. The resulting MPA-KCC-1 and IMPA-KCC-1 catalysts demonstrated excellent activity for the etherification of 5-HMF with ethanol, producing 5-(hydroxymethyl) furfural diethyl acetal (HMFDEA) and 5-(ethoxymethyl)furfural diethyl acetal (EMFDEA) products selectively. The significant advantages of the work are the selective production of EMFDEA at 82%, the catalyst can be easily removed via filtration, and the catalyst activity remains nearly intact even after five reaction cycles.Keywords:biomass;acetylation;5-HMF;etherification;heteropolyacid;fibrous silica
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危险品化学品经营许可证（带存储）