INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Haifeng Sun,Dinglingge Cao,Hao Wu,Huan Liu,Xue Ke,Tianyuan Ci
Tumor metastasis is the primary obstacle in cancer treatment and is always the leading cause of human death. And heparin and its derivatives are potential anti-metastatic agents with good biocompatibility. In this work, low molecular weight heparin (LMWH) based LMWH-Cholesterol (LHC) conjugates were prepared for intravenous delivery of doxorubicin (DOX). The DOX/LHC nanoparticles (DOX/LHC NPs) exhibited a spherical shape with a mean diameter of 135.5 ± 2.2 nm and had a longer circulation time than that of DOX. The in vitro results confirmed that the DOX/LHC NPs was more effectively taken up by 4T1 cells and showed a stronger anti-metastatic effect by cell invasion and cell migration compared with DOX. Meanwhile, DOX/LHC NPs also exhibited superior anti-metastatic effects in the pulmonary metastasis model compared with other groups. The reason may be account for the synergistic effect between the cytotoxic drug of DOX and its drug carrier of LMWH based nanoparticles, which is capable of anti-metastatic and anti-angiogenic efficiency. Thus DOX/LHC nanoparticles could be a promising anti-metastatic drug delivery system for postoperative chemotherapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2018
·Vol. 6
·期 8
·2172-2188
·
IF 5.831
Haifeng Sun,Dinglingge Cao,Yanhong Liu,Hui Wang,Xue Ke,Tianyuan Ci
Tumor metastasis has become a major obstacle for the clinical treatment of malignant breast cancer. Thus, a delivery system capable of both antitumor and anti-metastasis efficacy is desired. In this work, a low molecular weight heparin (LMWH)-based reduction-sensitive delivery system was developed, aiming to combine the properties of both the antitumor drug and active excipients to achieve a synergistic antitumor and anti-metastatic efficiency as well as resolving the potential adverse effects of both doxorubicin and LMWH. That is, the efficiency of DOX could be enhanced and its toxicity could be lowered. Meanwhile, the biological properties of LMWH could be strengthened and its bleeding risk could also be alleviated. Briefly, drug-loaded crosslinked nanoparticles (DOX/cLLHC2) had a longer blood circulation time and exhibited a rapid reduction-triggered release of DOX. The in vitro assays revealed that DOX/cLLHC2 exhibited a higher cellular uptake and cytotoxicity compared to free DOX. Meanwhile, for the existence of LMWH, DOX/cLLHC2 inhibited cell migration and invasion, as well as effectively inhibited the tube-like formation of human umbilical vein endothelial cells. DOX/cLLHC2 possessed a superior tumor accumulation compared to lipoic acid unmodified nanoparticles and free DOX. Also, DOX/cLLHC2 exhibited encouraging antitumor and anti-metastasis efficiency in an orthotopic breast cancer model. Overall, DOX/cLLHC2 could exert antitumor, anti-metastasis, and anti-angiogenesis efficacy simultaneously as well as having lower systemic toxicity, which makes it suitable for the therapy of metastatic breast carcinoma.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2018
·
IF 13.709
Xuanrong Sun,Guowei Wang,Hao Zhang,Shiqi Hu,Xin Liu,Jianbin Tang,Youqing Shen
Polymer micelles are one of the most investigated nanocarriers for drug delivery; many have entered clinical trials and some are in clinic use, but their delivery systems have not yet shown the expected high therapeutic efficacy in clinics. Further understanding their in vivo behaviors, particularly how quickly and by what mechanism polymer micelles are cleared (i.e., via micelles or unimers) once injected, is key to solving this dilemma. Herein, we hope to answer these questions for the clinically relevant polyethylene glycol-block-poly(ε-caprolactone) (PEG-PCL) and PEG-block-poly(d,l-lactide) (PEG-PDLLA) micelles. A small fraction of the hydrophobic chain ends was conjugated with a pair of fluorescence resonance energy transfer (FRET) dyes, Cy5 and Cy5.5, and used to fabricate FRET micelles whose FRET efficiency was correlated to the percentage of polymer chains in the micelles, the micelle degree. In vitro, serum proteins induced PEG-PCL micelle dissociation to some extent; mouse serum or blood surprisingly did not induce micelle dissociation but once with shear applied by a microfluidic channel caused most PEG-PCL micelles dissociated. After intravenous administration in mice, the PEG-PCL or PEG-PDLLA micelles were quickly sequestered into the liver as unimers, and the micelle degree in the blood quickly decreased to about 20%. The FRET-imaging experiments showed that in blood vessels the micelles quickly dissociated into unimers, which were found associated with albumin in blood and in liver. Thus, it is concluded that, upon intravenous injection, the shear and the bloodborne proteins (particularly albumin) induced the most (∼80%) PEG-PCL and PEG-PDLLA micelles to quickly dissociate into unimers, which were sequestered by Kupffer cells, while intact micelles were difficult to clear. These micelles were able to penetrate tumors and were very stable with cell membranes, but dissociated gradually inside cells. These findings on in vivo micelle fate and the clearance mechanism are directional for the rational design of polymer micelles for improved therapeutics; particularly, improving micelle stability in blood is the prerequisite for surface functionalizations such as introducing targeting ligands.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Ning Li,Di Wu,Na Hu,Guangsen Fan,Xiuting Li,Jing Sun,Xuefeng Chen,Yourui Suo,Guoliang Li,Yongning Wu
The present study reported a facile, sensitive, and efficient method for enrichment and determination of trace polycyclic aromatic hydrocarbons (PAHs) in food samples by employing new core–shell nanostructure magnetic covalent organic framework hybrid microspheres (Fe3O4@COF-(TpBD)) as the sorbent followed by HPLC-DAD. Under mild synthetic conditions, the Fe3O4@COF-(TpBD) were prepared with the retention of colloidal nanosize, larger specific surface area, higher porosity, uniform morphology, and supermagnetism. The as-prepared materials showed an excellent adsorption ability for PAHs, and the enrichment efficiency of the Fe3O4@COF-(TpBD) could reach 99.95%. The obtained materials also had fast adsorption kinetics and realized adsorption equilibrium within 12 min. The eluent was further analyzed by HPLC-DAD, and good linearity was observed in the range of 1–100 ng/mL with the linear correlation being above 0.9990. The limits of detection (S/N = 3) and limits of quantitation (S/N = 10) for 15 PAHs were in the range of 0.83–11.7 ng/L and 2.76–39.0 ng/L, respectively. For the application, the obtained materials were employed for the enrichment of trace PAHs in food samples and exhibited superior enrichment capacity and excellent applicability.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Tianfu Wei,Zhengyi Chen,Gongke Li,Zhuomin Zhang
Aflatoxins are highly toxic mycotoxin contamination, which pose serious food safety incidents. It is very important to precisely and rapidly determine trace aflatoxins in food. In this study, we designed porous monolithic column based on covalent cross-linked polymer gels for online extraction and analysis of trace aflatoxins in food samples with complicated matrices coupled with high-performance liquid chromatography-ultraviolet detector (HPLC-UV). The prepared monolithic column showed excellent enrichment performance due to its good permeability, good reproducibility and long life span. The study of adsorption mechanism suggested that the excellent enrichment performance of this monolithic column was attributed to the multiple effect of π-π stacking interaction, hydrophobic effect and steric effect. When the online analytical method was applied for the determine of trace aflatoxins in real food samples, aflatoxins G 1 and aflatoxins B 1 could be actually found in one positive bean sauce sample and quantified to be 32.8 and 26.4 μg/kg, respectively. Aflatoxins G 1 in one bean sample could be also found and quantified to be 25.9 μg/kg. The low detection limits of the developed method were achieved in range of 0.08-0.2 μg/kg. And the recoveries for spiked samples were in range from 76.1 to 113% with RSDs of 1.1-9.6%. The developed method was proved to be a promising method for online enrichment and analysis of trace aflatoxins in complicated food samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Xiao Sun,Jing Li,Li Xu
In the present study, the narrow-dispersed penetrable poly(methacrylic acid- co -ethylene glycol dimethacrylate) (poly(MAA- co -EDMA)) microspheres were successfully synthesized based on the sacrificial support method. The poly(MAA- co -EDMA) microspheres mirrored the porous structure of the sacrificial support, i.e. penetrable silica, characteristic of copious mesopores and throughpores. In addition, they possessed large surface area, adjustable hydrophobicity and the cation-exchange ability. Owing to their multi functionalities, they were applied as chromatographic stationary phase to separate proteins in different separation modes, including reversed phase, hydrophobic interaction and weak cation exchange. Moreover, thanks to their throughpores, fast separation at low column backpressure could be achieved in these three modes. Both protein recovery and column stability were satisfactory. The penetrable poly(MAA- co -EDMA) microspheres were potential stationary phase matrix for fast protein separation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY AND ECOLOGY
·
2018
·
IF 1.091
Tingfeng Liu,Keqiang Ding,Guang Guo,Feng Yang,Lihong Wang
Effect of hydroxypropyl-β-cyclodextrin (HPCD) on the bioavailability and biodegradation of the polycyclic aromatic hydrocarbons (PAHs) pyrene (PYR) and benzo[α]pyrene (BaP) in spiked soils was investigated in 14-week incubation experiments. To evaluate the effect of HPCD in soils with a different matrix, humic substance (HS) was added into soil samples. A 6-h Tenax TA extraction method was used to evaluate pollutants bioavailability. The biodegraded and extracted fractions were compared to evaluate the impact of HPCD on PAHs biodegradation. Results indicated positive effects of HPCD on fast desorption behaviours of PAHs. The biodegraded fraction was consistent with that of the extracted for PYR. However, in terms of BaP, the results were contrary which suggests that biological factors may be limiting factors for BaP pollution remediation. HS weakened the HPCD solubilisation effect while accelerated the decay of PYR and BaP, also implying that bioavailability was not the sole factor limiting PAH biodegradation. In addition, analysis of microbial communities demonstrated that HPCD inhibited the growth of some soil bacteria while HS promoted the evolution of some soil microorganisms. A limited population of hydrocarbon degrader populations led to observing incomplete PAH biodegradation even in the presence of HPCD.
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Xingru Hu,Chaohai Wang,Jiansheng Li,Rui Luo,Chao Liu,Xiuyun Sun,Jinyou Shen,Weiqing Han,Lianjun Wang
Developing novel coating materials for fast and sensitive solid-phase microextraction (SPME) is highly desired but few are achieved. In this work, a new material of metal–organic framework (MOF)-derived hollow carbon nanocubes (HCNCs) was prepared as a fiber coating material for SPME. The HCNC-coated fiber (denoted as HCNCs-F) exhibited a better enrichment performance than solid carbon nanocube (SCNC)-coated fiber (denoted as SCNCs-F) and commercial fibers based on the abundant active sites of the hollow structure, hydrophobic interactions, and π–π interactions. Moreover, because of the reduced mass-transport lengths of the hollow mesoporous structure, the HCNCs-F demonstrated a faster mass transfer compared with the SCNCs-F. The HCNCs-F was used to determine the six hydrophobic polycyclic aromatic hydrocarbons (PAHs) with wide linear ranges (10–2000 ng L–1 for naphthalene and 5–2000 ng L–1 for the other five analytes), good reproducibility (relative standard deviation < 8.8%), and low detection limits (0.03–0.70 ng L–1). Finally, the HCNCs-F was successfully applied for the determination of PAHs from the real water samples. It can be concluded from the results that MOF-derived hollow carbon materials are promising candidates for the fast SPME and can be used for practical applications in analytical chemistry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2018
·
IF 1.712
Xiaoqiang Jia,Yun He,Lei Huang,Dawei Jiang,Wenyu Lu
The high-molecular weight polycyclic aromatic hydrocarbons (PAHs) pyrene and typical long chain alkane n -hexadecane are both difficult to degrade. In this study, n -hexadecane and pyrene degrading strain Rhodococcus sp. T1 was isolated from oil contaminated soil. Strain T1 could remove 90.81% n -hexadecane (2 vol%) and 42.79% pyrene (200 mg·L − 1 ) as a single carbon within 5 days, respectively. Comparatively, the degradation of pyrene increased to 60.63%, but the degradation of n -hexadecane decreased to 87.55% when these compounds were mixed. Additionally, identification and analysis of degradation metabolites of Rhodococcus sp. T1 in the above experiments showed that there were significant changes in alanine, methylamine, citric acid and heptadecanoic acid between sole and dual substrate degradation. The optimal conditions for degradation were then determined based on analysis of the pH, salinity, additional nutrient sources and liquid surface activity. Under the optimal conditions of pH 7.0, 35 °C, 0.5% NaCl, 5 mg·L − 1 of yeast extract and 90 mg·L − 1 of surfactant, the degradation increased in single or dual carbon sources. To our knowledge, this is the first study to discuss metabolite changes in Rhodococcus sp. T1 using sole substrate and dual substrate to enhance the long-chain alkanes and PAHs degradation potential.
✓ 已验证
产品关联
DOI
高影响因子
CHEMISTRY OF MATERIALS
·
2018
·
IF 9.89
Xin Ye,Yang Liu,Quanxiang Han,Chao Ge,Shuangyue Cui,Leilei Zhang,Xiaoxin Zheng,Guangfeng Liu,Jie Liu,Duo Liu,Xutang Tao
Organic single crystals manifest the intrinsic physical properties of materials. However, traditional growth of organic single crystals is limited by low solubility from solutions or complexity from physical vapor deposition. Here we report a new method to grow organic single crystals by microspacing in-air sublimation, which avoids costly vacuum system and time-consuming procedures and is practical for a wide range of organic crystals. In situ crystal growth observation revealed an unprecedented vapor-to-melt-to-crystal mechanism, resulting from the micrometer scale spacing distance between the source and the growth position. FET devices based on the rubrene crystals directly grown on Si/SiO2 substrate exhibited higher mobility than the best record using SiO2 as the gate dielectric. This effective organic crystal growth technique can be affordable and handled for almost every lab, which may be beneficial for future research and application of organic crystals.
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 20
·17091-17095
·
IF 3.201
Youyou Pang,Rujian Zhao,Yao Lu,Jiyang Liu,Xiaoping Dong,Fengna Xi
Simple anti-counterfeiting printing using biocompatible, non-toxic and highly efficient luminescent materials plays crucial roles in economic and social fields as well as in our daily lives. In this work, new nitrogen doped graphene quantum dots (N-GQDs) are synthesized and applied in the preparation of quick-dry fluorescent ink for both writing and printing. The N-GQDs are synthesized based on a one-step bottom-up molecular fusion between 1,3,6-trinitropyrene and triethylamine by a solvothermal process with a high yield of 84.6%. Triethylamine simultaneously acts as a reaction medium and a nitrogen-doping agent for the formation of fluorescent N-GQDs. The as-prepared N-GQDs exhibit a single-layer graphene structure, high crystallinity, uniform size and excitation-independent fluorescence emission with a narrow half-peak width (66 nm). Successful doping of N atom in GQDs enables bright blue fluorescence with a high absolute photoluminescence quantum yield (17.8%). Owing to their excellent photostability and good solubility in ethanol, N-GQDs are applied to easily prepare quick-dry fluorescent ink for both writing and printing by simply blending GQDs with glycerol in an ethanol medium. Moreover, synthesis of a fluorescent powder and hydrogel is also achieved.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 56
·32193-32199
·
IF 2.936
Yiyuan Tang,Kai Chen,Jiacheng Li,Yuhong Feng,Gaobo Yu,Longzheng Wang,Xinyu Zhao,Yang Peng,Quan Zhang
In this study, a pH-responsive amphiphilic alginate (Ugi-Alg) was synthesized via Ugi reaction without using a catalyst. The structure of Ugi-Alg was confirmed by FT-IR and 1H NMR spectroscopy. Amphiphilic alginate can form micelles in an aqueous medium due to it's amphiphilic nature.. The impacts of Na+ concentration and pH on the micelle size were characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The dynamic light scattering observations showed that micelle size increases with the decrease in Na+ concentration in aqueous solution. However, the micelle size decreases first and then increases as the pH value decreases from 5.3 to 2.0. Transmission electron microscopy confirmed that the mean size of micelles is 30–200 nm. In addition, a model hydrophobic pesticide (acetamiprid) was loaded in the micelles. The encapsulation efficiency and release behavior of micelles were studied, which could be controlled by Na+ concentration and pH. The results indicated that encapsulation efficiency of acetamiprid increases from 55% to 96% due to the increase in Na+ concentration from 0.01 M to 0.3 M. Moreover, with the decrease in pH from 5.3 to 2.0, encapsulation efficiency increases from 55% to 80%. Furthermore, the data of acetamiprid release kinetics could be well-fitted by the Weibull model.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）