Journal of Central South University
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2014
·Vol. 21
·期 11
·4115-4120
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IF 0.464
Li Xin-she,Liu Zheng-yan,Lu Bu-shi
Ultrasound was applied for the extraction of pectin from chaenomeles . The content of pectin was evaluated by carbazole colorimetric method. Based on the results of the single factor experiment and the orthogonal tests, the optimum extraction parameters are as follows: the solid-to-liquid ratio is 1:4 g/mL, the ultrasonic power is 320 W, the ultrasonic-assisted extraction temperature is 60 °C, the ultrasonic-assisted extraction time is 50 min, and pH value is 2.0. Compared with acid hydrolyze methods, the extraction time of the present technique decreases from 70 min to 50 min, and the extraction yield increases from 1.39% to 2.61%. The results show that ultrasonic-assisted extraction is time-saving and highly efficient, so it provides a new way to extract pectin from chaenomeles .
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Polymer Chemistry
·
2015
·Vol. 6
·期 15
·2879-2891
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IF 5.52
Junfei Zhao,Yanyan Zhou,Yiwen Li,Xiangqiang Pan,Wei Zhang,Nianchen Zhou,Ke Zhang,Zhengbiao Zhang,Xiulin Zhu
Modular and precise construction of topological polymers with structural complexity is a prerequisite to understand the structure-property relationships of functional non-linear macromolecules. In this article, several examples of well-defined topological polymers are prepared by using two kinds of functionalized cyclic polymeric precursors via different high-efficient thiol-related chemistries. Firstly, multifunctional atom transfer radical polymerization (ATRP) initiators bearing alkyne and disulfide groups were elaborately prepared. Well-defined thiol-containing monocyclic and fused-bicyclic polymers via ATRP and Cu-catalyzed azide–alkyne cycloaddition (CuAAC) ring closure were then synthesized. Finally, by the utilization of thiol-containing monocyclic or fused-dicyclic polymers as building blocks, several kinds of macromolecules with tadpole-, spiro-shaped, fused-dicyclic tadpole and other topologies were successfully constructed via a modular mode. This study expands the scope of available macrocycle-based topological polymers and facilitates further diverse development of even more complex non-linear macromolecules via modular synthetic methodology.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Chunyan Sun,Rui Sun,Yuqing Chen,Yun Tong,Jiawei Zhu,Hong Bai,Shuyu Zhang,Hongru Zheng,Haiqing Ye
A fluorescent assay for Hg 2+ was developed based on magnetic separation and the formation of T-Hg 2+ -T base pairs. The aptamers were immobilized on the surfaces of magnetic beads to form aptamers-functionalized magnetic beads (AMB). In the absence of Hg 2+ , the signal transduction probe (STP) can combine with AMB through complementary base pairing. There is almost no STP in the detection solution after magnetic separation, which results in the fluorescence signal quite weak. The presence of Hg 2+ leads to the formation of T-Hg 2+ -T base pairs between aptamers and help DNA. After magnetic separation, Hg 2+ is separated from the system and STP is left in the solution, so the fluorescence signal was significantly enhanced. The fluorescence intensity linearly increased with the increasing of Hg 2+ concentration from 2 to 160 nM with the detection limit of 0.2 nM. This method has been successfully applied to test and quantify Hg 2+ in river water and ribbon fish with satisfactory recoveries, and the results were in full agreement with those from the atomic fluorescence spectroscopy (AFS). As-built method could avoid the direct binding of Hg 2+ with the fluorescent signal probe. Therefore, combining with magnetic separation, the heavy metal-fluorescence quenching effects could be effectively eliminated.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2017
·Vol. 409
·期 24
·5757-5765
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IF 3.431
Kong Rong-Mei,Zhang Xiaobin,Ding Lu,Yang Daoshan,Qu Fengli
Fluorescent light-up probes based on aggregation-induced emission (AIE)-active molecules have recently attracted great research interest due to the intelligent fluorescence activation mechanism and high sensitivity. In this work, an AIE-silica nanosphere (SiO 2 NP)-based label-free fluorescent aptasensor for the sensitive “turn-on” detection of prostate-specific antigen (PSA) is reported for the first time. The positively charged amino-functionalized SiO 2 NPs were used as efficient nanocapturer to electrostatically adsorb single-stranded PSA aptamer (PA) to form SiO 2 NP-PA nanocomposite as well as adsorb negatively charged tetraphenylethylene derivative 3 (TPE3) to form AIE-SiO 2 NP nanocomposite. The binding of the aptamer to the target PSA could induce a rigid aptamer conformation, resulting in the release of the PA away from the surface of SiO 2 NPs. This made the AIE molecules TPE3 aggregate on the SiO 2 NP surface and emit high fluorescence. With the advantages of simple design and rapid responses, the proposed aptasensor showed high sensitivity and selectivity for PSA with a detection limit of 0.5 ng/mL. The aptasensor was further applied in human serum samples with satisfactory results. Given its versatility, high selectivity, and sensitivity, the proposed method could be extended to other targets by varying the recognition probes.
An AIE-SiO 2 NP-based label-free fluorescent aptasensor for the sensitive “turn-on” detection of PSA is reported for the first time
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ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Huixia Di,Huiqiao Liu,Mingmin Li,Jin Li,Dingbin Liu
Precise profiling of the sialic acid (SA) expression on the membrane of cancer cells is critical for early identification of cancers and assessment of cancer metastasis. However, the complex physiological environments often result in false positives with currently available imaging technologies. Herein, we have established a background-free surface-enhanced Raman scattering (SERS) imaging platform that allows high-precision profiling of SA expression in cancer cells and differentiation of clinically relevant cancer tissues with various metastasis degrees. Three-dimensional Raman imaging technique provided a deeper insight into visualizing the probe distribution and thus the SA expression at the single-cell level, without destructing the cells. This noninvasive, high-precision imaging technique could favor early diagnosis, staging, and monitoring therapeutic responses of cancers that are highly essential in clinical settings.
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MATERIALS CHEMISTRY AND PHYSICS
·
2017
·
IF 2.084
Yiru Jia,Yi Pan,Haiying Wang,Rong Chen,Hua Wang,Xinjian Cheng
A fluorescent monomer of boron dipyrromethene (BODIPY) derivative ( 9AnPD) is designed and synthesized at first, and then it is introduced into polyurethane to obtain polymeric chemosensor with dangling fluorescent side groups. The monomer chemosensor 9AnPD exhibits highly selective properties to Hg 2+ ion in a wide pH range. The color of the monomer changed rapidly upon the addition of Hg 2+ ion. The fluorescence peak shifted and is enhanced about 4-flod. In addition, the monomer chemosensor reaches the detection limit of Hg 2+ is as low as 5 × 10 −6 m. When the fluorescent monomer is introduced to polymers, the sensing properties of the monomer chemosensor 9AnPD still remained. The polymer chemosensors not only exhibit fluorescence shift and color change for identifying Hg 2+ ion as its monomer chemosensor, but also improve the efficiency of detection. The detection limit of polymer chemosensor could reach 5 × 10 −7 m , and the fluorescence enhanced about 6-flod. The glass transition temperature (T g ) of the fluorescent PU is increased with the introduction of 9AnPD . However, it is about 60 °C, and will not lead to any adverse effect for the process. The molecular weights of PUs are lower than which without 9AnPD , but still of the macromolecular level.
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Biomaterials Science
·
2017
·Vol. 5
·期 4
·792-799
·
IF 4.21
Yi Hu,Leilei Shi,Yue Su,Chuan Zhang,Xin Jin,Xinyuan Zhu
Fluorescent light-up probes with aggregation-induced emission (AIE) characteristics have been focused on recently. In this report, a new fluorescent probe, namely, DEVD-TPE, which consisted of the substrate peptide Asp-Glu-Val-Asp (DEVD) and the AIE reporter group tetraphenylethene (TPE), was developed for detecting caspase-3 in living cells. In a slightly alkaline solution, the DEVD-TPE probe displayed almost no fluorescence owing to the dynamic rotation of the phenyl rings in solution. However, DEVD-TPE exhibited significant fluorescence when it was cleaved by caspase-3, as well as when the reporter group TPE underwent aggregation. The epidermal growth factor receptor (EGFR) inhibitor gefitinib was used for determining the screening efficacy of the probe for different non-small cell lung carcinoma (NSCLC) cell lines, namely, HCC827, A549 and H1650 cells. Cell proliferation and apoptosis assays indicated that the three cell lines had different sensitivities to gefitinib. The results of analysis by living-cell fluorescence imaging and flow cytometry were consistent with those of the cell proliferation and apoptosis assays. This demonstrated that our probe could detect caspase-3 in living cells, which confirmed the apoptosis of NSCLC cells. Furthermore, our probe indicated that gefitinib was more efficient against HCC827 cells than against the other two NSCLC cell lines. This report proves that the fluorescent probe DEVD-TPE is highly sensitive to caspase-3 and has potential prospects in the rapid screening of NSCLC.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Huixing Li,Xiong Shuai,Yanyan Chen,Jiaxing Xiong,Zhongxing Zou,Shuping Peng,Fangwei Qi,Cijun Shuai
Electrical stimulation displayed tremendous potential in promoting nerve regeneration. However, the current electrical stimulation therapy required complex traversing wires and external power sources, which significantly limited its practical application. Herein, a self-powered nerve scaffold based on primary battery principle was gradient printed by laser additive manufacturing technique. Specifically, poly-L-lactide (PLLA) containing Ag 2 O and Zn nanoparticles was prepared as the positive and negative electrode of the scaffold respectively, and PLLA/PPy was prepared as the middle conductive segment. In simulated body fluid, the negative electrode underwent oxidation to lose electrons and become positively charged. The lost electrons were transferred to the positive segment in a directed and orderly manner via the middle conductive segment, causing the positive electrode to be enriched electrons and become negatively charged. Subsequently, two segments can generate a potential difference to form an electric field, further generating current. Not merely, the redox process can release Ag + and Zn 2+ to endow the scaffold with antibacterial properties. Results showed that the scaffold could generate a current of up to 17.2 μA, which promoted a 14-fold increase in calcium ion influx and increased the mRNA expression of neuronal markers MAP2 by 24-fold. Moreover, the antibacterial rates of the scaffold against E. coli and S. aureus could reach 92.6 % and 91.9 %, respectively.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Qi Li,Guizhou Liu,Shixiang Zhou,Sihan Tang,Ruiying Luo,Peng Wei,Chun Fang,Chunze Yan
Utilizing reversible Zn metal anode is the most attractive way to meet the urgent demand for rechargeable Zn-ion batteries with high energy density. For reliable and stable zinc-ion batteries, it is of particular importance to understand and prevent the issues associated with the chemically reactive, ionically blocking and mechanically unstable interfaces/interphases of the Zn metal anode. It is well known that increasing the surface area of electrode can create a more uniform ion/electron field, facilitating uniform ion deposition. However, larger surface area often exacerbates corrosion issues, and the impact of the geometric structure of electrode on ion diffusion-nucleation-growth mode remains unclear. Herein, a fast ion transport network enhanced 3D Zn anode prepared by 3D printing is reported to regulate the electrochemistry of Zn. 3D structure design help to explore the impact of geometric constraints on ion diffusion-nucleation behaviors, validating boundary conditions of nucleation theories, so as to achieve a dense and uniform deposition morphology. And the Zn(OTF) 2 -PEO hydrogel components within the 3D Zn powder electrode construct a 3D ion transport network. The hydrogel network optimizes the nucleation process by reducing the surface energy, which can address the corrosion issue caused by the increased surface area.
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ACS Applied Nano Materials
·
2024
·
IF 5.3
Junjie Zhang,Yansheng Wu,Li Zhang,Wenran Gao
Nitrogen-doped graphene has been regarded as one of the most promising materials for nonradical peroxymonosulfate (PMS) activation. However, facile construction of nitrogen-doped graphene and systematic investigation of its nonradical PMS activation in a practical environment still far lag behind. Herein, graphitic N-rich graphene was prepared by one-step pyrolysis of urea. The obtained urea-derived graphitic N-rich graphene (UGNG) possessed a high N content of 12.12 at. % and a graphitic N proportion of 41.12%, giving a high graphitic N content of 4.98 at. %. We confirmed that PMS donated electrons to the graphitic N in UGNG, resulting in the cleavage of PMS to singlet oxygen for nonradical oxidation. The turnover frequency for the oxidation reached the highest value of 10.5 min–1. Also, the reaction could be conducted in a wide temperature (5–45 °C) range, even with the existence of common matrix species and different natural water matrixes. This work presented the direct conversion of N-rich organic urea to UGNG and systematically investigated its nonradical PMS activation, which is believed to be helpful to its further practical use.
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TALANTA
·
2024
·
IF 5.6
Jingwen Zhao,Zhuxuan Shi,Mixia Chen,Fengna Xi
Inspired by the iron porphyrin structure of natural horseradish peroxidase (HRP), an efficient carbon-based nanozyme was fabricated using nitrogen-doped graphene quantum dots (NGQDs) and iron ion (Fe 3+ ) nanocomposite, enabling selective distinguishment of hydroquinone (HQ) from its isomers. NGQDs with good dispersibility and uniform size were synthesized via a one-step hydrothermal process. NGQDs lacked peroxidase-like activity but the formed nanocomposite (Fe 3+ -NGQDs) upon Fe 3+ addition possessed high peroxidase-like activity. Fe 3+ -NGQDs nanocomposite exhibited shuttle-shaped structure (∼30 nm), the lattice structure of NGQDs and electron transfer between Fe 3+ and NGQDs. The Fe 3+ -NGQDs nanocomposite can catalyze the production of superoxide radicals (•O 2 − ) from H 2 O 2 . The Michaelis constant ( K m ) of Fe 3+ -NGQDs (0.115 mM) was lower than that of natural HRP (0.434 mM) with 3,3′,5,5′-tetramethylbenzidine (TMB) as the substrate and the maximum initial reaction rate ( V max , 16.47 × 10 −8 M/s) was nearly 4 times higher than that of HRP using H 2 O 2 substrate. HQ, unlike its isomers catechol (CC) and resorcinol (RE), could consume •O 2 − generated from the decomposition of H 2 O 2 catalyzed by Fe 3+ -NGQDs nanocomposite, reducing the oxidation of TMB. This principle enabled selective colorimetric determination of HQ ranged from 1 μM to 70 μM and a limit of detection (LOD) of 0.2 μM. Successful determination of HQ in pond water was also realized.
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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2024
·
IF 11.2
Junbing Zhu,Wenxi Hu,Jiangfeng Ni,Liang Li
Rechargeable zinc-ion micro-batteries (ZIMBs) are promising candidates for powering various microelectronic devices, although they typically exhibit low areal energy of ∼0.1 mWh cm − ². In this study, we reported the design and assembly of zinc micro-batteries through an ink extrusion-based printing approach. In the process, a three-axis printer was adopted, allowing for the fabrication of microelectrodes with large areal loading and diverse morphologies. Vanadium oxide anchored with carbon nanotubes was employed as the cathode material, zinc powder was used as the anode material, and a polyacrylamide gel containing ethylene glycol was selected as the electrolyte. The printed micro-batteries demonstrate an areal capacity of 0.51 mAh cm − ² and an energy density of 0.37 mWh cm − ² at a charge-discharge current of 0.2 mA, outperforming state-of-the-art zinc counterparts. This work provides a simple yet efficient solution to the development of high-energy micro-batteries.
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