ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Mengxing Cui,Qianmei He,Ziwei Wang,Yongjiang Yu,Huan Gao,Ziqi Liu,Honghao Peng,Han Wang,Xue Zhang,Daochuan Li,Liping Chen,Xiumei Xing,Yongmei Xiao,Wen Chen,Qing Wang
Micro/nano-plastics (MPs/NPs) are a newly discovered environmental pollutant that can be ingested by humans through food and drinking water. In this study we evaluated the impact of MPs/NPs on the intestinal barrier and its mechanism. Doses of MPs/NPs were used to treat Caco-2/HT29-MTX in-vitro model and in-vivo model. In in-vitro model, 20 nm polystyrene nanoplastics (PS-NPs) had higher cytotoxicity than larger particles (200 nm and 2000 nm), and led to the increase of the permeability along with the decreased expression of tight junction proteins. Intriguingly, 20 nm PS-NPs elevated the expression of MUC2 simultaneously. Further studies revealed that PS-NPs increased the expression of HO1 through ROS generation, and then activated p38 to elevate IL-10 secretion in Caco-2 cell. The IL-10 secreted by Caco-2 cell promoted the expression of MUC2 in HT29-MTX cell through STAT1/3. Elevated MUC2 expression alleviates the cytotoxicity of PS-NPs. Besides, increased intestinal permeability and up-regulation of MUC2 through Ho1/p38/IL-10 pathway was also observed in 20 nm PS-NPs treated mouse model. In conclusion, PS-NPs can induce the intestinal toxicity and result in the increased adaptive expression of MUC2 to resist this adverse effect. People with inadequate mucin expression need to pay more attention to the toxicity of PS-NPs. This study provided a valuable insight for clarifying the mechanism and potential risk of intestinal toxicity induced by nanoplastics.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES
·
2022
·
IF 3.848
Xiaojun Yuan,Qi Wang,Qing Zhao,Zehua Bai,Chen Chen,Haifeng Xie
The activation of matrix metalloproteinases (MMPs) is responsible for degradation of the hybrid layer . The effect of 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP) on biodegradation of the hybrid layer remains unknown. This study aimed to evaluate the effects of 10-MDP and its Ca salt on MMP-9 activity and dentin-bonding durability. 10-MDP-Ca salt was synthesized and characterized by X-ray diffraction (XRD) and nuclear magnetic resonance (NMR). The effects of chlorhexidine (CHX), 10-MDP, and 10-MDP-Ca salt on MMP activity were evaluated by in-situ zymography, MMP-9 inhibition assay, and hydroxyproline (HYP) assay. Dentin-resin bonded specimens were fabricated with using CHX or 10-MDP, including Single Bond 2 (Ctr), CHX + Single Bond 2 (CHX), 10-MDP + Single Bond 2 (10-MDP), and SBU (SBU). Micro-tensile bond strength (μTBS) and nanoleakage tests were performed after 24-h or two-years water storage. XRD and NMR confirmed the presence of 10-MDP-Ca salt. The relative inhibition percentages of recombinant MMP-9 for 10-MDP and 10-MDP-Ca salt were 8.04 ± 0.83% and 55.96 ± 1.82% respectively. HYP assay showed that 10-MDP, CHX, and 10-MDP-Ca salt conditioning decreased collagen degradation. Water storage weakened μTBS of all the groups, with the Ctr and CHX groups showing more significant decreases ( P < 0.05). After aging, the Ctr and CHX groups also presented more nanoleakage, and the CHX group showed more in-situ zymography fluorescence than the 10-MDP and SBU groups. The capacity to inhibit MMP-9 activity, decrease HYP release, and reduce nanoleakage of 10-MDP and its salt contribute to improvement of dentin-bonding durability.
✓ 已验证
产品关联
DOI
高影响因子
DENTAL MATERIALS
·
2022
·
IF 5.687
Xin Jin,Fei Han,Qi Wang,Xiaojun Yuan,Qiyue Zhou,Haifeng Xie,Lina Niu,Chen Chen
Objectives 10-Methacryloyloxydecyl dihydrogen phosphate (MDP) has been regarded as the most effective dentin-bonding monomer for more than 20 years. Although the dentin-bonding promoting effect of MDP has been well demonstrated, the mechanisms by which it benefits the stably of collagen within the adhesive–dentin hybrid layer are not currently fully understood. The objective of this study was to investigate the roles of MDP and its calcium salt in preserving the adhesive–dentin hybrid layer. Methods MDP-conditioned collagen was investigated by Fourier-transform infrared spectroscopy, Ultraviolet–visible spectroscopy, and molecular docking. The structural changes to the dentin surface upon acid-etching and MDP-conditioning were observed by SEM. X-ray diffraction and nuclear magnetic resonance were used to investigate the chemical interactions between MDP and HAp. The collagen-protecting effects of MDP and its Ca salt were investigated using in-situ zymography, rhMMP-9 colorimetric assay, hydroxyproline assay, and molecular docking. Results MDP forms a stable collagen-phosphate complex through hydrogen bonding with the collagen in dentin. Furthermore, it generates MDP-Ca salts that are deposited on the dentin collagen scaffold, protecting it from degradation. Moreover, both free MDP and the MDP-Ca salt inhibit matrix metallopeptidase and exogenous proteases, with the inhibitory effect of the calcium salt being significantly stronger than that of the free form. Significance MDP-based adhesives preserve the collagen within the hybrid layer by simultaneously improving collagen’s resistance to exogenous enzymes and inhibiting MMP activity, both of which contribute to the longevity of dentin–resin bonding.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemistrySelect
·
2022
·Vol. 7
·期 28
·e202201584
·
IF 2.307
Cheng-hui Wang,Hong-qian Zou,Hang Cong,Ying Huang,Zhu Tao,Carl Redshaw,Xin Xiao
Graphical A fluorescent probe based on supramolecular assembly between acridine orange (AO) and cucurbit[10]uril (Q[10]). Subsequent addition of dodine (DD) displaces AO from the Q[10] cavity, and results in a fluorescent output. Results revealed that the system is capable of the selective recognition of DD. The interaction between acridine orange (AO) and cucurbit[10]uril, Q[10] affords the ternary complex 2AO@Q[10]. Subsequent addition of dodine (DD) displaces AO from the Q[10] cavity, and results in a fluorescent output. The effect of 20 different pesticides, as well as a number of common interferents, on the fluorescent output is presented. Results revealed that the system is capable of the selective recognition of DD, with a limit of detection (LOD) for DD of 1.12×10 −7 M.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Qihong Zhang,Chen Su,Zhaohui Lu,Hui Wang,Zongmiao Feng,Alexandr V. Dushkin,Weike Su
An amphiphilic conjugate of carboxymethyl xylan-nonanoic acid (CX-NA) was synthesized with molecular weight of 38.35 kDa, HLB value of 13.59, and critical micelle concentration of 23.17 μg/ml. CX-NA could efficiently encapsulate the model drug of 10-hydroxycamptothecin (HCPT). The drug loaded amphiphilic conjugate could self-assembled to micelles with an average diameter of 110 nm, zeta potential of −42.88 mV, and drug encapsulation efficiency of 79.8%. In vitro experiments confirmed that the drug-loaded micelles exhibited excellent stability and permeability in the intestinal environment. Transport pathway demonstrated that HCPT was uptake by cells through clathrin-mediated endocytosis. Intestinal in situ absorption study further confirmed CX-NA vehicle could enhance HPCT to transport across intestinal epithelial cells in colonic tissues. Furthermore, the formulation showed excellent anti-tumor activity in vitro and improved bioavailability of 3.4 times in vivo as comparing with free HCPT. These findings imply that this amphiphilic conjugate is a potential and promising vehicle for delivery anticancer drug.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2021
·
IF 7.561
Wu Binxin,Gao Furong,Lin Jianhua,Lu Lixia,Xu Huiming,Xu Guo-Tong
Allergic conjunctivitis (AC) is the most prevalent form of mucosal allergy, and the conditioned medium (CM) from mesenchymal stem cells has been reported to attenuate some allergic diseases. However, the therapeutic effects of CM from different tissue stem cells (TSC-CM) on allergic diseases have not been tested. Here, we studied the effects of topical administration of different human TSC-CM on experimental AC (EAC) mice. Only human amniotic epithelial cell-CM (AECM) significantly attenuated allergic eye symptoms and reduced the infiltration of immune cells and the levels of local inflammatory factors in the conjunctiva compared to EAC mice. In addition, AECM treatment decreased immunoglobulin E (IgE) release, histamine production, and the hyperpermeability of conjunctival vessels. Protein chip assays revealed that the levels of anti-inflammatory factors, interleukin-1 receptor antagonist (IL-1ra) and IL-10, were higher in AECM compared to other TSC-CM. Furthermore, the anti-allergic effects of AECM on EAC mice were abrogated when neutralized with IL-1ra or IL-10 antibody, and the similar phenomenon was for the activation and function of B cells and mast cells. Together, the present study demonstrated that AECM alleviates EAC symptoms by multiple anti-allergic mechanisms mainly via IL-1ra and IL-10. Such topical AECM therapy may represent a novel and feasible strategy for treating AC.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Fan Xiao,Ming Cao,Rongrong Chu,Xiaoyu Hu,Wenxiong Shi,Yingbo Chen
A new type of composite membrane was prepared through the vacuum filtration self-assembly, in which, graphene oxide (GO) was the basic material, and the horizontal insertion material is product of perylene-3, 4, 9, 10-tetracarboxylic dianhydride (PTCDA) and UiO-66-NH 2 (PTCDA-UiO-66-NH 2 ). The leading role of Π-Π conjugate, auxiliary effect of hydrogen bonding during membrane preparation have been confirmed through Fourier transform infrared spectroscopy (FTIR), UV–visible spectrophotometer, Raman spectroscopy, and X-ray diffraction (XRD). The prepared GO@PTCDA-UiO-66-NH 2 membrane had new nodular structure compared to GO membrane by scanning electron microscopy (SEM) and atomic force microscopy (AFM), which promoted the water transport. In addition, the insertion of PTCDA-UiO-66-NH 2 narrowed the actual filtration spacing between GO sheets, and PTCDA-UiO-66-NH 2 could also adsorbed dye laterally. Experiments showed that the permeance of GO@PTCDA-UiO-66-NH 2 membrane was 1.7 times of GO membrane, and the removal of methyl blue, congo red, crystal violet and disperse black 9 was close to 100%. Under extreme pH, high salt concentration and multiple recycling, its separation ability was still excellent. The GO@PTCDA-UiO-66-NH 2 membrane constituted a unique synergistic structure of vertical-screening and horizontal-adsorption, which successfully overcame the trade-off effect and obtained excellent stability of structure and performance. Therefore, GO@PTCDA-UiO-66-NH 2 membrane had great potential in practical applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2021
·Vol. 23
·期 1
·1-12
·
IF 2.253
Zhang Gongguo,Liu Zhiang,Fu Xiaowei,Zhao Wenjun,Yin Xunxiao,Niu Xiankang,Kong Rongmei,Qu Fengli,Zheng Yiqun
Branched Au nanocrystals with sub-10-nm arms are synthesized via a one-pot reduction in aqueous phase. The success of current work relies on the use of octadecyltrimethylammonium chloride as the shape-directing capping agent to facilitate the formation of branched products in high purity. Their overall size could be controlled in the range of 16–60 nm by varying the Au precursor amount or concentration of capping agent, and the diameter of arms is limited to sub-10-nm size range. Interestingly, Au nanocrystals with a unique morphology, sheet-wrapped dendrites, could be obtained when the concentration of a reductant was varied. The current strategy is also validated effective in the fabrication of dendritic products composed of other noble metals or alloys in addition to Au by simply using the alternative metal precursor. As compared to spherical counterparts, the current branched Au nanocrystals exhibited noticeable red-shift in UV-Vis extinction spectrum and enhanced catalytic activities for electrooxidation of ethanol in alkaline media. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2020
·
IF 2.369
Yanmin Shen,Wenju Liu,Chen Sun,Paifeng Shi
The solid–liquid phase equilibrium solubility of doxycycline hyclate (DH) in 10 pure organic solvents and cosolvent mixtures DMF (1) + ethanol (2), DMSO (1) + ethanol (2) and acetone (1) + ethanol (2) at a temperature range from 278.15 to 323.15 K was measured and discussed. The results indicated that the experimental mole fraction (x) of DH in 10 neat solvents followed this order: acrylic acid > acetic acid > 1-butanol > 1-pentanol > tetrahydrofuran > isobutyl alcohol > isopropyl alcohol> propanoic acid > acetone > acetonitrile, and temperature affected obviously the solubility especially in acetic acid. Moreover, the experimental mole fraction (x) of DH increased with mass concentration rising of DMF or DMSO in DMF (1) + ethanol (2) or DMSO (1) + ethanol (2), and decreased gradually with mass concentration rising of acetone in acetone (1) + ethanol (2). The experimental solubility in all solvents was correlated better by the modified Apelblat model according to lower values of RAD and RMSD.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2020
·
IF 2.888
Chunhao Guo,Yang Tang,Jinru Wan,Yue Zuo,Yong Wang,Yichang Wang,Xunqiu Wang
The isobaric vapour–liquid equilibrium (VLE) data for the binary system of cyclohexanol + cyclohexylbenzene were determined at (5, 10, 15, 20 and 25) kPa with a dynamic recirculating apparatus and the temperature ranged from 358.10 K to 456.43 K, and the thermodynamic consistency of the experimental data was checked by the Herington area method and Van Ness method. Afterwards, the experimental data was correlated with NRTL, Wilson and UNIQUAC model, and the corresponding interaction of thermodynamic models of the binary system parameters were obtained through the Aspen software. The results indicated that all three models represent the experimental values quite well, while the NRTL model provided a slightly better result than Wilson model and UNIQUAC model. The maximum root mean square deviation of the temperature and vapour phase mole fraction for the NRTL model was less than 0.4 K and 0.004, respectively.
✓ 已验证
产品关联
DOI
高影响因子
DESALINATION
·
2020
·
IF 7.098
Weiwei Zhang,Hui-Jing Li,Liwei Chen,Jiani Sun,Xingyi Ma,Yunfei Li,Chao Liu,Xu Han,Boyi Pang,Yan-Chao Wu
An environment-friendly scaling–corrosion inhibitor composed of 10-methylacridinium iodide (MAI) and sodium citrate (SC) was developed, whose scale inhibition performance were studied through weight loss, electrochemical techniques , static tests and surface analysis techniques (SEM, SECM , XPS , XRD). The optimum mass ratio of MAI and SC corrosion inhibition was found to be 1:2, and the optimum mass ratio of scale inhibition was 1:3. The experimental results showed that MAI–SC mixture as mixed-type scaling–corrosion inhibitor with excellent corrosion ( η w = 92.7%) and scale inhibition performance ( η Ca = 98.3%). The electrochemical stability of the inhibitor film was investigated by chronoamperometry (CA). SEM and XRD simulations indicated that the MAI–SC mixture inhibitor can distort and disperse the crystal lattice of calcium carbonate without forming scale, and it plays a dominant role in the inhibition of calcite (110) surface growth. The structure-activity relationship between the molecule structure of the MAI–SC mixture and its scale–corrosion inhibition performance was discussed based on the quantum chemistry calculation.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Lulu Zhang,Zhenning Yan,Yamin Lv,Shuangxia Shen
In the present work, three new pharmaceutical salts based on N -alkyl-pyridinium ([C n Py] + , n = 8, 10, 12) as the cations and acetyl-sulfacetamide ([AceSA] - ) as an anion were synthesized. The thermal stability was measured by synchronous thermal analyzer. The solvation and micellization behaviors in aqueous solution were investigated by experimental density and conductivity data at different temperatures. These data were used to calculate the apparent molar volume at infinite dilution, V 2 , φ 0 , the apparent molar expansibility at infinite dilution, E φ 0 , limiting molar conductivity, Ʌ 0 , critical micelle concentration, cmc , and the relative thermodynamic functions for the micellization of [C n Py][AceSA] in aqueous solution. The effect of the aliphatic chain length of N -alkyl-pyridinium cation and temperature on the estimated properties was examined. The obtained results confirmed that [C n Py] + (n = 8, 10, 12) cations with longer alkyl chain exhibit higher decomposition temperature, large V 2 , φ 0 , low cmc and Ʌ 0 values. The results were discussed in terms of solute–solvent interactions and structural changes of [C n Py][AceSA] in water.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）