Chemistry-An Asian Journal
·
2012
·Vol. 8
·期 1
·276-281
·
IF 4.5
Jian Chen,Huping Jiao,Wenying Li,Dongli Liao,Huipeng Zhou,Cong Yu
Graphical A desired breakdown : A novel tetracationic perylene probe (probe 1 ) was designed and synthesized. Probe 1 was used for the real-time fluorescence turn-on assay of alkaline phosphatase (ALP) activity and inhibitor screening. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. en A tetracationic perylene probe (probe 2 ) was designed and synthesized. Probe 1 was used for the real-time fluorescence turn-on assay of alkaline phosphatase (ALP) activity and inhibitor screening. Probe 1 monomer fluorescence could be very efficiently quenched by ATP through the formation of an ATP/probe 1 complex. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. In the presence of an ALP inhibitor, a decrease in fluorescence recovery was observed. zh 1
研究领域:
分析化学
·
生物化学
·
分子生物学
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SN Applied Sciences
·
2020
·Vol. 2
·期 5
·1-8
·
IF 0
Gong Sheng,He Tianhao,Huang Qizhang,Shu Xugang,Zhou Xinhua
A thermo-responsive polymer, poly(N-isopropylacrylamide) (PNIPAM), was anchored on the surface of low-cost natural clay nanorods, attapulgite (ATP), through the interactions including hydrogen bonding and coordination. Premixing the monomers of NIPAM with ATP before polymerization caused that the obtained PNIPAM could distribute among the pore voids of ATP and function as a surface affinity conditioner in response to temperature change. The PNIPAM/ATP composite was then applied in temperature-triggered adsorption and desorption of a hydrophobic drug (typically, an agricultural pesticide: acetamiprid). The results indicated a satisfactory adsorption capacity and reusability with the strong combination of PNIPAM and ATP. Furthermore, the adsorption mechanism was discussed, and the Sips model was employed to explain the adsorption behavior. It was proved to be beneficial for hydrophobic drug loading as well as its delivery, which may also have a potential to extend to other natural products and responsive polymers.
研究领域:
材料科学
·
药物递送
·
高分子化学
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Decai Wang,Tong Zhou,Yalan Cui,Chen Bao,Shuaiqi Yuan,Chengchen Wu,Qian Liu,Chao Liu,Jiannan Hu,Mingrong Liu,Ziling Li,Xinyue Ma,Yunfei Zhu,Jingjing Sun,Shuyun Xu,Yuzhou Wu
The persistent opening of the mitochondrial permeability transition pore (mPTP) plays a critical role in bronchial asthma pathogenesis. The ATP synthase c subunit (c subunit) constitutes a core component of mPTP. A novel c subunit inhibitor, 1,3,8-triazaspiro [4.5] decane derivatives (PP10), effectively suppresses pathological mPTP opening without impairing ATP synthesis. Although intraperitoneal PP10 administration mitigates airway inflammation in asthmatic mice, its hydrophobicity hinders inhaled delivery to airway epithelial mitochondria, requiring penetration of the mucus layer, cell membrane, and mitochondrial outer membrane. To overcome this, we developed inhalable human serum albumin-triphenylphosphine-polyethylene glycol-PP10 nanoparticles (HSA-TPP-PEG-PP10 NPs). These NPs demonstrated efficient mucus penetration, high drug loading, mitochondria-targeting capability, and biosafety. They suppressed house dust mite/lipopolysaccharide (HDM/LPS)-induced mPTP opening, inhibited the mitochondrial DNA (mtDNA)-cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway, reduced inflammation in human bronchial epithelial (HBE) cells, and alleviated airway inflammation in asthmatic mice upon inhalation.
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Advanced Healthcare Materials
·
2026
·
IF 11
Si-Yi Li,Ying-Jie Zhu,Heng Li,Han-Ping Yu
The high stability of storage and high mineralization efficiency are pivotal for the restoration of acid-etched dental enamel, yet these demands are mutually exclusive and difficult to be met. Herein, we propose a bioinspired “dormancy-to-activation” mineralization strategy by synthesizing “dormant” adenosine 5’-triphosphate (ATP)-stabilized amorphous calcium phosphate (ACP) nanoparticles as the precursor that can be activated and accelerated by saliva for rapid crystallization to form hierarchically ordered crystalline hydroxyapatite nanorods (mineralization). To realize this goal, we have designed and constructed a bioinspired dormant-ATP-stabilized ACP nanoparticle system with high stability and controllable mineralization ability using ATP biomolecules as the stabilizer. The ACP-ATP nanoparticles can maintain the dormancy for mineralization in vitro, which can stabilize the amorphous structure of ACP nanoparticles for 25 days in water. In the oral environment, the alkaline phosphatase (ALP) in saliva acts as a biological signal to activate and accelerate the mineralization of ACP-ATP amorphous nanoparticles. More importantly, the coating of ACP-ATP amorphous nanoparticles on the acid-etched dental enamel can effectively restore the hierarchically ordered structure, recover mechanical properties, and replicate fracture-resistance behaviors of natural dental enamel.
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Advanced Science
·
2026
·
IF 14.1
Zhijun Liu,Haihong Lai,Qing Yang,Chaoyue Zhang,Bin Jia,Qiyi Chen,Yuling Deng,Fen Cao,Yuyu You,Zhe Li,Dongming Kuang,Bo Li
Tumor-associated macrophages (TAMs) arise from monocytes and represent major contributors to the immunosuppressive microenvironment of solid tumors. However, the environmental cues that govern TAM differentiation and immunosuppressive activity remain incompletely understood. Here we demonstrate that hepatocellular carcinoma (HCC) cells secrete extracellular vesicles (EVs) that are preferentially taken up by monocytes, inducing their differentiation to TAMs characterized by a distinct immune-inhibitory signature. Mechanistically, HCC-derived EVs encapsulate the lipogenic enzyme ATP-citrate lyase (ACLY), promote palmitate biosynthesis in targeted monocytes, thereby enhancing the S-palmitoylation and stability of multiple immune checkpoint proteins. To validate this, we synthesized liposomal vesicles (LVs) decorated with an EV-marker protein CD81, which mimicked the targeting specificity of endogenous EVs for monocytes and differentiated macrophages. When loaded with ACLY proteins as interior cargo, these LVs were sufficient to induce immunosuppressive TAMs and promote HCC progression. Conversely, CD81-decorated LVs encapsulating the ACLY inhibitor SB204990 markedly reduced the TAM-mediated immunosuppressive activity, leading to restrained HCC progression. Importantly, we further demonstrated that targeting EV-transferred, TAM-specific ACLY represents a promising strategy to enhance immunotherapeutic efficacy without notable side effects, particularly when combined with anti-PD-1/PD-L1 antibodies for HCC treatment.
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ANALYTICAL CHEMISTRY
·
2026
·Vol. 98
·期 33
·24079-24092
·
IF 7.3
Chi Jinxin,Xie Qian,Xie Shulun,Luo Dexin,Lin Xucong,Huang Guihua
The precise quantification of adenosine triphosphate
(ATP) at physiologically
critical femtomolar levels in complex biological fluids remains a
formidable challenge, constrained by irreconcilable demands for extreme
sensitivity, specificity, and operational robustness. Conventional
integrated platforms, such as LC-SERS, often suffer from functional
fragmentation, in which separation and detection operate sequentially
without synergy, thereby limiting overall performance. Stand-alone
amplification strategies, meanwhile, are intrinsically hampered by
matrix interference. Herein, we introduce a versatile biosensing paradigm
that overcomes these limitations through the synergistic integration
of two orthogonal amplification mechanisms within a unified microfluidic
platform. Our dual-signal amplification microfluidic platform (2Amp-MFP)
integrates online micro high-performance liquid chromatography (μHPLC)
for target pre-enrichment and interference removal with a nanozyme-catalyzed
SERS aptasensor (NC-SERS aptasensor) for ATP recognition and catalytic
signal generation. The upstream amino-silica monolith (ASM) provides
phosphate-dependent retention, allowing adenosine diphosphate (ADP),
adenosine monophosphate (AMP), and matrix components to be removed
during loading and washing, while triphosphate species are retained
and subsequently eluted with a Mg 2+ -containing mobile phase.
In the downstream aptasensor, ATP is selectively recognized, triggering
the displacement of a fraction of the aptamer-conjugated Au@Pt nanozymes
from the sensor interface. After washing, the Au@Pt@Aptamer NPs remaining
hybridized on the monolith catalyze the oxidation of 3,3 ′ ,5,5 ′ -tetramethylbenzidine
(TMB) to generate Raman-active reporter oxTMB, yielding a SERS intensity
that decreases with increasing ATP concentration. Under optimized
conditions, the 2Amp-MFP achieved a limit of detection (LOD) of 63
fM for ATP and enabled specific quantification of spiked ATP in human
serum and urine samples. This synergistic dual-signal amplification
strategy provides a promising platform for trace ATP analysis in complex
biological matrices.
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Fermentation-Basel
·
2025
·Vol. 11
·期 12
·667
·
IF 3.3
Yutong Lu,Huayang Tang,Dexun Fan,Qingzhu Wang,Shuangyan Han
D-Allulose is a promising low-calorie rare sugar with significant health benefits. However, its industrial production is hindered by the low catalytic efficiency (≤33% conversion) and unfavorable equilibrium of the key enzyme, D-allulose 3-epimerase (DAE). To overcome this thermodynamic bottleneck, an in vitro synthetic enzymatic cascade based on a phosphorylation–dephosphorylation strategy was constructed. This engineered system comprises four synergistically operating enzymes: D-allulose-3-epimerase (DAE), L-rhamnulose kinase (RhaB), polyphosphate kinase (PPK), and acid phosphatase (AP). Through rational design and systematic optimization, the cascade achieved an exceptional 84.5% conversion yield from 50 mM D-fructose. Importantly, the system also maintained high conversion rates of 64.4% and 61.1% at high D-fructose loadings (50–100 g L−1). This performance, together with the integration of a low-cost PolyP6–PPK ATP regeneration module, underscores the potential industrial applicability of the proposed cascade strategy.
研究领域:
生物催化
·
酶工程
·
合成生物学
·
工业生物技术
·
碳水化合物化学
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POLYMER BULLETIN
·
2023
·Vol. 81
·期 1
·969-993
·
IF 3.2
Li Chen,Zhen Weijun
Modified attapulgite (ATP-PS) grafted with polystyrene (PS) was prepared based on Pickering emulsion polymerization. The characterization of ATP-PS was studied by transmission electronic microscopy (TEM), X-ray photoelectron spectrometer (XPS), Fourier transform infrared spectrometer (FTIR) and contact angle analysis. TEM analysis showed that the surface of ATP-PS was coated with PS organic layer; XPS analysis showed that the proportion of C element in ATP-PS was much larger than that of ATP; FTIR analysis found that ATP-PS had more PS specific functional group peaks than that of ATP; Moreover, contact angle results showed that the hydrophobicity of ATP-PS was significantly improved compared with ATP. The above characterization analysis proved that the modification of ATP-PS was successful. Moreover, polyphenylene sulfide (PPS) hydrophobic porous membrane was prepared with 1-chloronaphthalene and dibutyl phthalate as mixed diluents and ATP-PS as additives. The results showed that 0.8 wt% of ATP-PS had a great effect on the structure and performance of the membrane. With the increasing of the mass proportion of ATP-PS in the preparation of PPS base membrane, the microstructure of PPS based membrane was changed from blocky bicontinuous to spherical granular bicontinuous. When the addition amount of ATP-PS was 0.8 wt% of the total mass of PPS base membrane, PPS based membrane had strong hydrophobicity and organic solvent affinity, and its n -butanol flux could reach 106.9642 L m −2 h −1 bar −1 . The rheological properties of PPS based membrane showed that ATP-PS was well compatible with the PPS, and ATP-PS was beneficial to crosslink between PPS molecular chains, thereby increasing the mechanical properties of PPS based membrane. Phase transition analysis demonstrated that ATP-PS (0.8 wt%) increased the melting temperature (2.34 °C) and crystallization temperature (2.63 °C) of PPS based membrane compared to PPS, thus allowing PPS to complete the phase transition at higher temperature interval during film formation, resulting in a stable microscopic cluster structure. Based on hydrophobic porous PPS membrane, PPS/ATP-PS/TA nanocomposites membrane with ATP-PS selective layer was prepared by co-deposition of tannic acid (TA) and ATP-PS on the surface of PPS membrane. The performance of PPS/ATP-PS/TA nanocomposites membrane was studied, which showed that the n -butanol flux of PPS/ATP-PS/TA nanocomposites membrane was 61.2913 L m −2 h −1 bar −1 . More importantly, the simulated filtration performance of MB dye organic waste liquid (the solvent is n -butanol) showed that the maximum rejection rate of MB dye reached 99.82%. These results demonstrate that PPS/ATP-PS/TA nanocomposites membrane will have potential application in the filtration of organic dye wastewater.
研究领域:
材料科学
·
高分子科学
·
化学工程
·
膜技术
·
环境科学
·
表面改性
·
纳米技术
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高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Zhihao Wu,Ruchun Chen,Shuang Pan,Hui Liu,Xiaoli Hu
Herein, a convenient method is developed for ratiometric fluorescence detection of ATP (adenosine triphosphate) based on CDs-OPD (carbon dots- o-phenylenediamine). In the absence of ATP, oxOPD, the colored oxidation product of OPD fabricated by a redox reaction occurred between OPD and Cu 2+ , not only quenches the fluorescence of CDs, but also emits a yellow fluorescence which acts as another signal element. However, ATP will compete with OPD to chelate Cu 2+ inhibiting the redox reaction, which hampers the formation of oxOPD resulting in the diminution of IFE (inner filter effect) between CDs and oxOPD, then the fluorescence of CDs restores. ATP-induced dual-signal response of the CDs-OPD/Cu 2+ system makes ratiometric assay possible. The method exhibits high sensitivity toward ATP with a detection limit of 0.43 μmol L −1 . Furthermore, the semi-quantitative measurement of ATP is available by naked eyes through the color of fluorescence and solution. Finally, the developed method is employed for the quantification of ATP in practical samples with satisfactory results.
研究领域:
分析化学
·
生物传感
·
纳米材料
·
分子生物学
·
临床检验
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Journal of Materials Chemistry B
·
2026
·
IF 6.2
Qingqing Wang,Kun Yan,Lin Zhu,Jiaxin Li,Sen Li,Yiyan Miao,Yansong Shen,Lei Cao,Haijiao Chen,Guiping Yu,Dihai Gu
Mitochondria serve as central regulators of tumorigenesis, progression, metastasis, and chemoresistance, playing a pivotal role in cancer bioenergetics and thus representing prime targets for anticancer therapy. However, conventional strategies aimed at disrupting mitochondrial energy supply often exhibit limited efficacy due to the compensatory interplay between mitochondrial tricarboxylic acid cycle (TCA) and glycolysis. Herein, we developed a trimetallic nano-trap capable of achieving synergistic ATP depletion via dual-pathway metabolic interference. Specifically, this engineered system co-delivers Cu-MOF and CaO2 to trigger irreversible Cu2+/Ca2+ dual-overload, resulting in structural damage and functional impairment of mitochondria, thereby suppressing ATP generation. Concurrently, glucose is progressively depleted through the catalytic activity of Au NPs, effectively interrupting the glycolytic energy supply. By concomitantly inhibiting glycolysis and mitochondrial metabolism, this system achieves a marked reduction in ATP production. This ion interference-based strategy offers a promising paradigm to overcome metabolic plasticity in cancer treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Huaqiang Li,Shuqiang Zhuang,Yang Yang,Fei Zhou,Jianhua Rong,Jianhao Zhao
The stability of self-assembled drug nanocarriers during blood circulation and the controlled intracellular drug delivery are two challenges in cancer therapy. In this paper, we constructed an adenosine triphosphate (ATP)/hyaluronidase(Hyals) dually responsive core-shell hyaluronan/chitosan-based drug nanocarrier for breast cancer therapy, using SNX-loaded 3-fluoro-4-carboxyphenylboronic acid-conjugated quaternary ammonium chitosan nanoparticles ( [email protected] NPs) as the core and crosslinked polyethylene glycol-/methacrylate-modified hyaluronic acid (mHA-PEG) as the shell. The formed [email protected] /mHA-PEG NPs were stable against salt ion strength, pH values and human plasma mimicking the bloodstream, but ATP/Hyals dually sensitive with a drug delivery of 85% within 48 h in the mimicking intracellular environment of breast cancer cells. These nanoparticles showed a low hemolysis of less than 3%, a high resistance to bovine serum albumin adsorption of 0.06 mg/mg, and an efficient internalization by two breast cancer cell lines (MCF-7 and MDA-MB-453). The cell culture indicated that they were friendly to human skin fibroblasts, but presented a close IC 50 value to SNX for MCF-7 (0.14 μg mL −1 ) and MDA-MB-453 (0.05 μg mL −1 ) at 48 h, respectively. Thus, [email protected] /mHA-PEG NPs were potential drug nanocarriers for breast tumor therapy.
研究领域:
药学
·
肿瘤学
·
生物材料
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RSC Advances
·
2018
·Vol. 8
·期 40
·22577-22582
·
IF 2.936
Fengying Dai,Mengjuan Wen,Jinna Wang,Wenzhe Jiang,Xuetao Tian,Yao Dong,Yiping Zhao,Li Chen
To detect and adsorb methylene blue (MB) from wastewater simultaneously, a solid fluorescent and absorbent material was designed by immobilizing attapulgite (ATP) on calcium alginate (CA) and reacting with carbon dots (CDs) which were modified by the activation of γ-(2,3-epoxypropoxy) propyltrimethoxysilane (KH-560), then the CA/ATP-g-CDs gel fibers were prepared. The problem of CDs easily falling out of materials was solved. The structures of the gel fibers were characterized by field emission scanning electron microscopy (FE-SEM), specific surface area (BET) and X-ray photoelectron spectroscopy (XPS). The thermal properties were analyzed by thermogravimetry (TG). The adsorption capacity was measured and the effect of initial pH was investigated. The results showed that ATP was successfully reacted with CA and the adsorption capacity was enhanced with the increase of the pH value. CA/ATP-g-CDs gel fibers were favorable materials to detect and adsorb MB simultaneously, and MB could be adsorbed by gel fibers and also the fluorescence of CA/ATP-g-CDs was weakened. At low concentrations of MB (1 μg L−1), the removal efficiency could even be as high as 100%.
研究领域:
材料科学
·
环境科学
·
化学工程
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高影响因子
危险品化学品经营许可证（带存储）