ACS Omega
·
2022
·
IF 4.132
Meng-Ting Song,Wen-Zhu Wang,Yao Lu,Rui-Min Han,Leif H. Skibsted,Jian-Ping Zhang
The interactions of luteolin (Lut) with bovine serum albumin (BSA) mediated by Cu(II) were investigated by spectroscopic, calorimetric, and molecular dynamic (MD) methods. Fluorescence studies showed that the binding of Lut to BSA was significantly enhanced by Cu(II) coordination with the number of binding sites and binding constant increasing from n = 1 and Ka = 3.2 × 105 L·mol–1 for Lut to n = 2 and Ka = 7.1 × 105 L·mol–1 for a 1:1 Cu(II)–luteolin complex, in agreement with the results from isothermal titration calorimetry (ITC). Site-specific experiments with warfarin and ibuprofen and MD confirmed that two binding sites of BSA were sequentially occupied by two Cu(II)–luteolin complexes. Cu(II) coordination increased the antioxidant activity of luteolin by 60% in the inhibition of carbonyl formation from the oxidation of amino groups in the side chain of BSA induced by the peroxyl radical ROO•; however, it counteracted the antioxidant effects of luteolin and played pro-oxidative roles in BSA aggregation induced by •OH.
研究领域:
生物化学
·
分子生物学
·
光谱学
·
量热法
·
分子动力学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 6
·1988-1994
·
IF 4.019
Qi Wang,Yuan Zhang,Xiaodong Wang,Yuehuan Wu,Chuan Dong,Shaomin Shuang
A MnO2 nanoparticle (MnO2 NP)-mediated fluorescent turn-on probe for sensitively and selectively detecting glutathione (GSH) and recognizing cancer cells was established in this work. MnO2 NPs were synthesized simply and quickly through an in situ redox reaction by mixing bovine serum albumin (BSA) and KMnO4, in which BSA served the dual roles of template and reductant. It was found that the MnO2 NPs served as an effective energy acceptor and quenched the fluorescence intensity of carbon dots (CDs), owing to the fluorescence resonance energy transfer (FRET) process. Further, the addition of GSH triggered the decomposition of MnO2, breaking the FRET between MnO2 NPs and CDs and thus restoring the fluorescence intensity of CDs. Based on this mechanism, quantitative determination of GSH was performed. Under optimal conditions, a satisfactory linear range of 0.05–90 μM and limit of detection of 39 nM were obtained, and GSH content in human serum samples was detected. Moreover, taking advantage of the higher levels of GSH in cancer cells than in normal cells, the MnO2 NP-CD probe was applied to distinguish SMMC-7721 cancer cells from L02 normal cells. The FRET was interrupted by GSH in cancer cells, and strong fluorescence was observed. This work provides a facile approach for synthesizing MnO2 NPs, and this rapid, low-cost method with no need for reductants makes synthesis green and convenient. The MnO2 NP-mediated fluorescent turn-on response to GSH could improve the MnO2 nanomaterial-based biochemical analysis applications.
研究领域:
纳米医学
·
肿瘤学
·
生物分析化学
·
分子探针
·
转化医学
·
氧化还原生物学
✓ 已验证
DOI
高影响因子
Nanoscale
·
2021
·Vol. 13
·期 18
·8531-8542
·
IF 7.79
Wenjing Hou,Yingzong Jiang,Guangchao Xie,Lu Zhao,Fangshi Zhao,Xuejun Zhang,Shao-Kai Sun,Chunshui Yu,Jinbin Pan
Hemorrhage transformation (HT) is a frequent but maybe fatal complication following acute ischemic stroke due to severe damage of the blood–brain barrier (BBB). Quantitative BBB permeability imaging is a promising method to predict HT in stroke patients for a favorable prognosis. However, clinical gadolinium chelate-based magnetic resonance (MR) imaging of the stroke suffers from a relatively low sensitivity and potential side effects of nephrogenic systemic fibrosis and intracranial gadolinium deposition. Herein, BSA–MnO2 nanoparticles (BM NPs) fabricated by a facile disinfection-mimic method were employed for the permeability imaging of BBB in the stroke for the first time. The BM NPs showed a high T1 relaxivity (r1 = 5.9 mM−1 s−1), remarkable MR imaging ability, and good biocompatibility, allowing the noninvasive timely visualization of BBB permeability in the model rats of middle cerebral artery occlusion (MCAO). Furthermore, increased peak intensity, extended imaging duration, and expanded imaging region indicated by BM NPs in MR imaging showed a good prediction for the onset of HT in MCAO rats. Therefore, BM NPs hold an attractive potential to be an alternative biocompatible MR contrast agent for the noninvasive BBB permeability imaging in vivo, benefiting the fundamental research of diverse neurological disorders and the clinical treatment for stroke patients.
研究领域:
神经学
·
医学影像学
·
纳米技术
·
中风研究
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yuao Feng,Zeliang Wu,Hui Liu,Rong Xu
Triple-negative breast cancer (TNBC) presents a formidable challenge due to its poorest prognosis and limited array of treatment options available. Photodynamic therapy (PDT) has emerged as a potent therapeutic modality to generate intratumoral toxic reactive oxygen species (ROS) in combating refractory triple-negative breast cancer (TNBC). However, its therapeutic efficacy is compromised due to insufficient tumor accumulation and therapeutic resistance. Herein, an “all-in-one” tumor-therapeutic nanomedicine named HA@IR780@KU55933@BSA (HIKB) which integrated photosensitizer IR780 with ATM kinase inhibitor KU55933 was designed to facilitate drug delivery and target specific pathways involved in tumor PDT treatment resistance. Co-delivery of IR780 and KU55933 exacerbated intracellular ROS production, mitochondrial dysfunction and DNA damage to form a potent anti-TNBC therapeutic cyclical feedback loop and then induced pyroptosis and apoptosis of TNBC cells by activating the Caspase3/GSDME signaling pathway and regulating apoptosis-related protein expression, respectively. In vivo evaluations in the TNBC orthotopic xenograft mouse model demonstrated that the designed HIKB NPs could accumulate in tumor tissues and exert synergistic therapeutic effects. Altogether, this study described a self-assembling strategy for constructing an all-in-one nanomedicine that effectively integrates multiple therapeutic modalities to provide a comprehensive and systemic approach to tumor suppression.
研究领域:
肿瘤学
·
治疗学
·
纳米技术
·
癌症生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Pharmaceutics
·
2025
·Vol. 17
·期 3
·376
·
IF 5.5
Yuxing Zhang,Xixi Zou,Qiran Du,Xiaotao Dong,Uday Kumar Chinta,Ruyue Yu,Fei Wu,Tuo Jin
Objectives: Thermo-gelling hydrophilic polymers like PLGA–PEG–PLGA are known as injectable sustained-release depots for biologics, but they face challenges due to the occurrence of severe burst release. This study aimed to develop a strategy to avoid the initial burst release by pre-encapsulating proteins in polysaccharide microparticles through an aqueous–aqueous emulsion mechanism, thereby enhancing therapeutic retention and linear release kinetics.Methods: Five model proteins (G-CSF, GM-CSF, IGF-1, FVIII, BSA) were encapsulated in dextran microparticles, using an organic solvent-free aqueous–aqueous emulsion method. These particles were dispersed in a 23% (w/w) PLGA–PEG–PLGA solution and injected into a 37 °C release buffer to form a gel depot. The in vitro release profiles were quantified using ELISA and MicroBCA assays over 9–42 days. The bioactivity of the proteins was validated using cell proliferation assays (NFS-60, TF-1, MCF-7) and chromogenic kits. The in vivo pharmacokinetics of the FVIII-loaded formulations were evaluated in Sprague–Dawley rats (n = 5/group) over 28 days.Results: Protein-loaded dextran particles retained their structural integrity within the hydrogel and exhibited minimal burst release (≤5% within 30 min vs. >25% for free proteins). Sustained near-linear release profiles were observed for all the proteins, with complete release by day 9 (G-CSF, GM-CSF, BSA) or day 42 (FVIII). Rats administered with the thermal gel with FVIII–dextran particles showed a significantly lower peak plasma concentration (Cmax: 88.25 ± 30.21 vs. 132.63 ± 66.67 ng/mL) and prolonged therapeutic coverage (>18 days vs. 15 days) compared to those administered with the thermal gel with the FVIII solution. The bioactivity of the released proteins remained at ≥90% of the native forms.Conclusions: Pre-encapsulation in dextran microparticles effectively mitigates burst release from thermosensitive hydrogels, while preserving protein functionality.
研究领域:
药剂学
·
生物医学工程
·
药物递送系统
·
蛋白质工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2020
·
IF 3.557
Xingyuan Wang,Xiansong Shi,Yong Wang
Covalent organic frameworks (COFs) are increasingly utilized as doping agents for the design of advanced ultrafiltration mixed matrix membranes, thanks to their prominent nanoporosity and excellent polymer compatibility. However, current strategies are largely limited in the complicated postaddition of neutral COF particulates. Herein, cationic COFs, namely, TpEB, with sizes down to ∼39 nm are in situ synthesized in polyacrylonitrile (PAN) solution as crystalline fillers for the production of highly permeable TpEB–PAN ultrafiltration membranes. After the condensation of monomer pairs, the growth of cationic TpEB crystallites is restrained due to the electrostatic interaction with negatively charged PAN chains, leading to the formation of a homogeneous TpEB-incorporated casting solution. During the subsequent nonsolvent-induced phase separation process, TpEB crystallites facilitate exchange between the solvent and the nonsolvent because of their hydrophilic and nanoporous nature, accelerating the rate of phase inversion to form a highly porous membrane surface. Thus-prepared TpEB–PAN membranes deliver a tight rejection of BSA with water permeance of up to 380 L m–2 h–1 bar–1, which is 35.6% higher than that of the original PAN membranes prepared without TpEB. The TpEB–PAN membranes also exhibit enhanced operation stabilities and fouling resistances. This in situ growth strategy suggests a new avenue for the preparation of advanced mixed matrix membranes.
研究领域:
材料科学
·
化学工程
·
膜技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Liubing Kong,Ying Gan,Tao Liang,Longjie Zhong,Yuxiang Pan,Dmitry Kirsanov,Andrey Legin,Hao Wan,Ping Wang
As a powerful tool for medical diagnosis and bioanalysis , conventional optical spectrometers are generally expensive, bulky and always require an accompanying data processing device. In this work, we developed a novel smartphone-based CD-spectrometer (SCDS) for high sensitive and ultra-portable colorimetric analysis , with the advantage of cost-effective and simplicity. The distance between the light source and slit, the structure of SCDS and the parameters of camera in the smartphone were all optimized to ensure the best analytical performance. Besides, the SCDS employed HSV color model and utilized the overall intensity calculated by summing V-value of adjacent position for the absorbance measurement. In this way the errors caused by the low resolution of CD-grating can effectively be eliminated to promote the sensitivity of the SCDS. The performance of the SCDS was first validated for colorimetric detection of BSA with a detection limit of 0.0073 mg/mL, which is superior compared to that of the microtiter plate reader (MTPR). Moreover, by combining with 3,3′,5,5′-tetramethylbenzidine-manganese dioxide (TMB-MnO 2 ) nanosheets reaction, a high sensitive and specific system for ascorbic acid detection was established. The SCDS gives a detection range from 0.6250 μM to 40 μM with a detection limit of 0.4946 μM for AA detection. Compared to other studies, the SCDS features wide detection range and very low detection limit with low cost instrument. Therefore, the SCDS will be an ideal and promising colorimetric system for point-of-care (POC) application in food security, disease diagnosis and environmental monitoring.
研究领域:
医学诊断
·
生物分析
·
分析化学
·
即时检测
·
环境监测
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Jing Zhang,Biao Shen,Liqun Chen,Lingdong Chen,Yangsheng Meng,Jie Feng
A multifunctional envelope-type mesoporous silica nanoparticle (MSN) was delicately designed for subcellular co-delivery of drug and therapeutic peptide to tumor cells. Firstly, a kind of cell apoptosis peptide (KLAKLAK) 2 (KLA) was anchored on surface of MSN via disulfide bond to obtain MSN-SS-KLA. Subsequently, anticancer drug doxorubicin hydrochloride (DOX) was loaded into the pores of MSN-SS-KLA. Then, the drug loaded MSN-SS-KLA (DOX@MSN-SS-KLA) was further coated with bovine serum albumin (BSA) to obtain a biological media stable MSN based drug delivery system (DDS), DOX@MSN-SS-KLA/BSA, for cancer synergetic therapy. The results show that stability of the DOX@MSN-SS-KLA/BSA is much better than that of DOX@MSN-SS-KLA and it could keep well dispersed in serum for more than 24 h. After accumulating at tumor site by EPR effect, the DOX@MSN-SS-KLA/BSA could be effectively phagocytosed by HeLa cells and release apoptotic peptide KLA as well as DOX simultaneously responding to reductive stimulus inside the cells. In vitro cell experiment results show that the DOX@MSN-SS-KLA/BSA complex exhibits much better inhibition on HeLa cells compared with pure DOX, indicating that co-delivery of KLA and DOX is expected to achieve synergetic therapy of cancer.
研究领域:
纳米技术
·
药学
·
癌症研究
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Yimu Qiao,Xue Han,Feiyong Chen,Cuizhen Sun,Linxu Xu,Rupeng Liu,Xue Shen
To guide the practical application of ozonation and adsorption coupled with ceramic membrane technology , the mitigation mechanisms of O 3 pre-oxidation, powdered activated carbon (PAC) pre-adsorption, and combined O 3 -PAC pretreatment on membrane fouling caused by natural organic matter (NOM) were investigated. The membrane and water quality were characterized by scanning electron microscopy (SEM), energy dispersive spectrometer (EDS), three-dimensional fluorescence-parallel factor analysis (PARAFAC-EEM), dissolved organic carbon (DOC), laser particle size and Zeta potential . Results showed that the membrane flux decline caused by humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and surface water were alleviated by 35.2%, 55.2%, 31.0% and 59.0% after O 3 -PAC pretreatment. Meanwhile the removal rates of HA, SA and BSA were 26.2, 74.8% and 42.3%, respectively. The combination of PAC and O 3 can effectively control the membrane fouling caused by HA and SA. Ozonation can degrade organic matter into small molecules, and PAC has high adsorption to these small molecules, by recycling PAC can take away this part of organic matter, thereby reducing membrane fouling. O 3 showed no obvious advantage for membrane fouling caused by BSA, and the decrease of membrane fouling was mainly attributed to the adsorption of PAC. This investigation highlights the potential mechanism of O 3 -PAC in alleviating the membrane fouling caused by different organic compounds, contributing to the efficient and low-carbon operation of the production.
研究领域:
环境工程
·
水处理
·
膜技术
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Yao Lu,Hong Ke,Yang Wang,Yao Zhang,Huan Li,Chusen Huang,Nengqin Jia
A ratiometric electrochemiluminescence resonance energy transfer (ECL-RET) platform depended on novel dye BODIPY derivatives was proposed for rapid detection of lactoferrin . This ECL-RET platform is composed of aptamer decorated BODIPY composites and C 60 @BSA, in which BODIPY derivative is the ECL probe and can generate significant resonance energy transfer with K 2 S 2 O 8 . BODIPY derivative and K 2 S 2 O 8 are used as built-in reference signal and calibration signal respectively to eliminate background signal and abnormal change signal by double signal self-calibration process. At the same time, C 60 , as the accelerator of K 2 S 2 O 8 , can effectively increase the ECL signal and further transfer as much energy as possible to BODIPY derivative. Under optimal conditions, the constructed ECL-RET platform exhibited sensitive detection of lactoferrin in the wide linear range of 10 -4 - 850 ng/mL with a LOD of 42 fg/mL. Meanwhile, the proposed ECL-RET aptasensor demonstrated superior stability, specificity and reproducibility, displaying favorable application value in practical diagnosis of this method.
研究领域:
分析化学
·
生物化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2013
·Vol. 37
·期 7
·2174-2178
·
IF 2.966
Xing Liu,Qi Wang,Yu Zhang,Lichun Zhang,Yingying Su,Yi Lv
In this paper, a novel and simple colorimetric method for the determination of glutathione (GSH) was developed. The BSA–MnO2 NPs possess oxidase-like activity that can catalyze the oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB), and the existence of GSH can cause reduction of oxidized TMB along with a visual color variation. A good linear relationship can be obtained from 0.26 to 26 μM with a limit of detection of 0.1 μM. Furthermore, the good recoveries for serum samples indicated that the present colorimetric methodology was feasible, simple and sensitive, with a promising application in routine analysis of biosamples.
研究领域:
分析化学
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
POLYMER ENGINEERING AND SCIENCE
·
2015
·Vol. 55
·期 12
·2829-2837
·
IF 1.52
Shushan Yuan,Juan Wang,Xiaojun Wang,Shengru Long,Gang Zhang,Jie Yang
Poly(arylene sulfide sulfone) (PASS) is a kind of newly developed polymeric membrane material which has excellent mechanical strength, thermal stability, and solvent resistance. And, it would be a potential material for high temperature ultrafiltration and organic solvent filtration. In this article, PASS hybrid ultrafiltration membrane with improved antifouling property was prepared by mixing TiO 2 nanoparticles which were grafted with polyacrylic acid (PAA). These membranes were prepared by a phase inversion technique and their separation performance and antifouling property of the prepared membranes were investigated in detail by SEM, FTIR, EDS, contact angle goniometry, filtration experiments of water, and BSA solution. The results shown that the TiO 2 - g -PAA nanoparticles dispersed well in membrane matrix, the hydrophilicity of the membranes prepared within TiO 2 - g -PAA nanoparticles have been improved and these membranes exhibited excellent water flux and antifouling performance in separation than that of the pure PASS membranes and PASS membranes with TiO 2 nanoparticles. More specifically, among membrane sample M0, M1.5, and MP1.5, MP1.5 which contained 1.5 wt% TiO 2 - g -PAA exhibited the highest water permeation (190.4 L/m 2 h at 100 kPa), flux recovery ratio, and the lowest BSA adsorption amount. POLYM. ENG. SCI., 55:2829–2837, 2015. © 2015 Society of Plastics Engineers
研究领域:
材料科学
·
化学工程
·
膜技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）