INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Jingqi Sun,Jiaxin Li,Yanzhi Xing,Han Leng,Wei Chen,Yanfeng Zhang,Xuwei Chen
Adenine DNA glycosylase (MutY) is a crucial member of DNA glycosylase family, and the abnormal expression of the human MutY homologs is associated with the pathogenesis of various diseases, therefore, convenient and cost-effective assessing the activity of MutY holds significant biological and medical importance. Herein, the precise identification/excision capacity of MutY to mismatched G-A base pair of dsDNA chains and the DNA-template-dependant fluorescence behaviors of copper nano cluster (CuNCs) was exploited for the accurate sensing of active MutY. Hairpin DNA with G-A base mismatch was excised by MutY to produce dsDNA chains with repetitive AAT-TTA base pairs. The newly formed dsDNA provided more active sites for the growth of CuNCs compared to the original hairpin DNA, resulting in the significantly enhanced fluorescence of final CuNCs. MutY was accurately quantified with a detection limit of 9.98 nmol L −1 . The developed sensing protocol exhibited excellent selectivity toward MutY over various ions, neutral biomolecules, and protein species. Most importantly, The sensing system is capable to distinguish the active MutY from other MutY homologs with low activity, e.g., de-[4Fe4S] cluster MutY (DIS-MutY), and the practicality was well demonstrated by detecting active MutY contents in various cell lysates.
研究领域:
生物化学
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分子生物学
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医学诊断
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2024
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IF 6.1
Yan Chen,Miao He,Feifan Yin,Wenting Cheng,Zhongyun Wang,Yang Xiang
Dipeptidyl peptidase IV (DPPIV) is an enzyme belonging to the type II transmembrane serine protease family that has gained wide interest in the fields of hematology, immunology, and cancer biology. Moreover, DPPIV has emerged as a promising target for therapeutic intervention in type II diabetes. Due to its biological limitations, traditional strategies cannot meet the requirements of low abundance DPPIV analysis in complex environments. In this work, combining the high programmability of DNA and the chemical diversity of peptides, we designed DNA–peptide conjugates that can be specifically recognized, polypeptides as specific substrates for target DPPIV and DNA probes as primers for catalytic hairpin assembly (CHA), recycling a large amount of DNAzymes by triggering CHA amplification. The DNAzyme substrate modified with the FAM fluorescent group was immobilized on the surface of gold nanoparticles by S–Au chemical bonds to form a signal output probe. The DNAzymes enzyme cleaved the substrate of the signal outputs probe, yielding a double-amplified fluorescence signal. This method has a detection limit as low as 0.18 mU mL−1 and a linear range of 0–5 mU mL−1 in serum samples, showing high stability and good potential for practical applications.
研究领域:
生物化学
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分子生物学
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分析化学
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糖尿病研究
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癌症生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2019
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IF 6.393
Rufeng Zhang,Yu Wang,Xiaonan Qu,Shasha Li,Yihan Zhao,Su Liu,Jiadong Huang
Herein, an Exonuclease III (Exo III)-powered DNA walking machine has been integrated into electrochemical sensing for label-free and highly sensitive detection of antibiotics. The electrochemical sensing interface is prepared by co-assembly of hemin aptamer-containing DNA track (DT) and DNA walker (DW)-locking probe (LP) duplex on gold electrode surface. The specific target and aptamer interaction triggers Exo III-assisted cyclic amplification, releasing the unlocking probe (UP) that is used for the deblocking of DW. Then the autonomous moving of DW on electrode surface is propelled via Exo III digestion of hybridized DT. The consumption of DT induces the formation of G-quadruplex/hemin DNAzyme that can catalyze the reduction of H 2 O 2 , thus a significantly strong current signal is released. Using ampicillin as a model analyte, the proposed biosensor achieves a detection limit of 0.76 pM within a linear detection range from 1 pM to 10 nM. Additionally, this biosensing strategy can be readily extended for the detection of wide variety of analytes by using corresponding target recognition probes. Therefore, the proposed strategy indeed creates a highly sensitive and label-free electrochemical sensing platform for molecular diagnosis applications.
研究领域:
生物化学
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分析化学
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分子诊断
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Jiajing Cai,Bei Xia,Haofei Ji,Xiuen Cao,Qubo Zhu,Chuanpin Chen
DNA methylation at specific CpG sites plays a critical role in the diagnosis and treatment of human diseases. In this study, we introduce a site-specific DNA methylation detection biosensor that leverages immune recognition and nonlinear hybridization chain reactions (NHCR). Since anti-methylcytosine antibodies are unable to recognize methylcytosine within a DNA duplex, we specifically target the CpG site by removing the guanine complementary to the target methylcytosine in the capture probe, thereby creating a protruding region accessible for antibody recognition. We utilized NHCR as a signal amplification strategy to enable site-specific DNA methylation detection under isothermal and enzyme-free conditions. The proposed method achieves a detection limit of 0.15 pM and can differentiate methylation levels as low as 0.1 %, even in the presence of a large excess of unmethylated DNA. This approach is capable of detecting DNA methylation at specific CpG sites in genomic DNA extracted from human hepatocellular carcinoma (HCC) tissues, showcasing its considerable potential for biological research and disease diagnosis.
研究领域:
分子生物学
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医学诊断
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表观遗传学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2018
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IF 5.123
Li Zou,Ruidi Shen,Liansheng Ling,Gongke Li
We developed a novel strategy for rapid colorimetric detection of specific DNA sequence based on gold nanoparticles assemblies induced by polymerase chain reaction (PCR) product. In the presence of target DNA , the two DNA-functionalized AuNP probes selectively hybridized with the prohibited nucleic acid segments of two primers owing to the zipping off of the hairpin structures during PCR process, resulted in the aggregation of AuNPs with a concomitant color change from red to blue-purple. It is a convenient and universal method for sensitive DNA detection with no need for any further post-treatment of the PCR products. Most importantly, our method showed a low limit of detection (LOD) of 4.3 fM with a wide range of target DNA from 16 fM to 1.6 nM. Owing to the versatility and low cost, the proposed strategy could be extremely useful for a wide range of applications, providing a promising tool for rapid disease diagnostics and gene sequencing.
研究领域:
生物化学
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分子诊断
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纳米生物学
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DOI
PubMed
高影响因子
BIOMACROMOLECULES
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2023
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IF 6.2
Cong Wei,Peipei Li,Lixin Liu,Hong Zhang,Tianyu Zhao,Yongming Chen
Following brain trauma, secondary injury from molecular and cellular changes causes progressive cerebral tissue damage. Acute/chronic neuroinflammation following traumatic brain injury (TBI) is a key player in the development of secondary injury. Rapidly elevated cell-free DNAs (cfDNAs) due to cell death could lead to production of inflammatory cytokines that aggravate TBI. Herein, we designed poly(amino acid)-based cationic nanoparticles (cNPs) and applied them intravenously in a TBI mice model with the purpose of scavenging cfDNA in the brain and suppressing the acute inflammation. In turn, these cNPs could effectively eliminate endogenous cfDNA, inhibit excessive activation of inflammation, and promote neural functional recovery.
研究领域:
神经科学
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药学
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生物医学工程
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Mingbing Yi,Wenjun Liu,Cong Wang
Lactate, a critical biomarker for various physiological and pathological conditions, necessitates precise quantification methods for effective disease diagnostics and monitoring. Traditional detection techniques, though effective, are often hampered by the need for complex instrumentation and procedures. Here, this study introduces a highly sensitive and selective electrochemiluminescence (ECL) aptasensor for the detection of lactate, leveraging the unique properties of DNA-terbium (Tb) nanoparticles (NPs) and aptamer technology. Specifically, in this label-free ECL sensor, DNA-Tb NPs were utilized as both ECL probes and recognition unit, which were synthesized by a simple coordination driven self-assembly of lactate aptamer ligand with terbium ions metal center. The biosensor exhibits a remarkable limit of detection (LOD) of 2.9 μ M across a linear range of 0.01 to 10 mM, demonstrating high selectivity, stability, reproducibility, and practical application potential. The work not only underscores the efficacy of DNA-Tb NPs in enhancing ECL signals but also showcases the aptamer’s role in achieving high specificity towards lactate detection. The optimization of assay conditions further elevates the biosensor’s performance, establishing it as a promising tool for the rapid, simple, and sensitive detection of lactate in various settings.
研究领域:
生物传感
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分析化学
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医学诊断
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DOI
高影响因子
Nature Communications
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2025
·Vol. 16
·期 1
·1-12
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IF 15.7
Chen Yonghuan,Li Xiuying,Yue Xinru,Yu Weihua,Shi Yuesen,He Zilong,Wang Yuanfeng,Huang Yu,Xia Fan,Li Fengyu
Synthetic drugs fundamentally reshape the illicit drug market due to their low cost, ease of production, and rapid manufacturing processes. However, current drug detection methods, which rely on complex instruments, have limited applicability and often neglect the influence of pH fluctuations, leading to potential bias and unreliable results. Herein, we propose co-calibration DNA probes on a nanoconfined biosensor (N C B S ), covering the range of sweat pH 3–8 to achieve significantly enhanced target signal recognition. The N C B S exhibits a linear response range of 10 3 -10 8 fM with a low limit of detection (LOD) of 3.58 fM in artificial sweat. Compared to the single-aptamer N C B S , the dual-aptamer N C B S offers a broader linear response range, primarily due to the synergistic effects of changes in surface wettability and the capture of hydrion, which together reduce signal interference in proton transport. The linear response range doubles, and its detection sensitivity improves by 4–5 orders of magnitude compared to existing drug detection methods. This sensing strategy expands the application scope of aptamer-based composite probes, offering an approach for ultra-sensitive drug detection and demonstrating significant potential in sweat sensing and drug monitoring fields.
研究领域:
生物传感
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分析化学
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药物检测
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DOI
PubMed
高影响因子
IEEE SENSORS JOURNAL
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2024
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IF 4.3
Lingling Li,Shen Yu,Chonglu Jing,Lihua Chen,Ai Zhou
In order to deal with the issue of low sensitivity to biomolecules concentrations in traditional optical fiber surface plasmon resonance (SPR) biosensors, a fiber SPR deoxyribonucleic acid (DNA) biosensor was developed, which is based on an amino-functionalized Zr-based metal–organic framework (MOF) (UIO-66-NH2)-modified heterogeneous core structure. Porous material UIO-66-NH2 facilitates electron transfer and amplifies the SPR effect, resulting in a heightened sensitivity for the detection of DNA hybridization. This can be attributed to the material’s advantageous characteristics, including a substantial specific surface area, an excellent water stability, and a pronounced affinity for probe-DNA (pDNA). The DNA biosensor presented in this study demonstrates enhanced sensitivity as a result of the covalent attachment of MOFs to pDNA, hence increasing the number of binding sites available for target DNA (tDNA). The optical fiber SPR biosensor, which has been modified with UIO-66-NH2, exhibits remarkable capabilities in detecting DNA concentrations ranging from 1 pM to 1 μM . It demonstrates a sensitivity of −7.72 nm/log ( μM ) and achieves a detection limit of 3 pM. In addition, the DNA biosensor has exceptional performance in terms of specificity and durability, rendering it a highly favorable option for utilization in several fields, such as drug delivery, protein identification, and environmental surveillance.
研究领域:
生物传感技术
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分子生物学
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DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
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2016
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IF 3.42
Qi Qin,Xue Ma,Xiali Liao,Bo Yang
As a prerequisite of gene delivery in living cells, DNA condensation has attracted more and more attention. In order to improve the efficiencies of polyamine-β-cyclodextrin-based cationic polyrotaxanes (PR-EDA and PR-DETA) as DNA condensation materials, we have designed and prepared two novel scutellarin-grafted cationic polyrotaxanes (PR-EDA-SCU and PR-DETA-SCU), in which scutellarins (SCU), the planar molecules, were conjugated on the cyclodextrin molecules of PR-EDA and PR-DETA. These materials were characterized by 1D and 2D NMR, XRD, TG and DSC. The electrophoresis assays showed that pDNA condensation efficiencies of PR-EDA and PR-DETA were better than that of PR-EDA and PR-DETA. The complexes of PR-EDA, PR-DETA, PR-EDA-SCU and PR-DETA-SCU with pDNA were further investigated by zeta potential and atomic force microscopy analysis. The results indicated that the planar structure of SCU played an important role in improvement of pDNA condensation efficiencies of PR-EDA-SCU and PR-DETA-SCU. The satisfactory pDNA condensation abilities of PR-EDA-SCU and PR-DETA-SCU could be helpful in designing non-viral gene delivery vectors to control gene expression and delivery.
研究领域:
生物化学
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分子生物学
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材料科学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2013
·Vol. 1
·期 46
·6418-6426
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IF 0
Wenyu Li,Peng Zhang,Kun Zheng,Qiaoling Hu,Youxiang Wang
Extracellular stability to protect DNA against nucleases and stimulus-triggered intracellular DNA release are key factors in designing non-viral gene vectors. In this study, the diselenide-linked polycation mPEG–SeSe–PEI was developed as a new type of PEG-detachable gene vector for redox-responsive gene delivery. The corresponding stable analog mPEG–PEI and the disulfide-linked polycation mPEG–SS–PEI were synthesized as controls. The results showed that all the PEGylated polycations could condense DNA into tightly packed spherical nanoparticles about 80 nm in size, which showed excellent stability under physiological conditions. The results of zeta-potential measurements, stability tests and DNA release ability assay indicated that at a GSH concentration of 0.3 mM, the diselenide bonds were more easily cleaved than disulfide bonds, which facilitated dePEGylation and DNA release. Meanwhile, it was interestingly found that mPEG–SeSe–PEI/DNA polyplexes showed higher gene expression than mPEG–SS–PEI/DNA polyplexes in both HEK293T and HepG2 cells. Confocal laser scanning microscope (CLSM) images revealed that mPEG–SeSe–PEI/DNA polyplexes showed more efficient endosomal escape ability than mPEG–SS–PEI/DNA polyplexes. Based on these results, the diselenide bonds as a novel strategy are more suitable to address the challenging problem of extracellular stability and intracellular DNA release.
研究领域:
基因递送
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聚合物化学
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分子生物学
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生物医学工程
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DOI
PubMed
APPLIED SURFACE SCIENCE
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2019
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IF 5.155
Yujie Qiang,Shengtao Zhang,Liping Wang
Deoxyribonucleic acid (DNA) was investigated for suppressing copper corrosion in 0.5 M H 2 SO 4 through electrochemical measurement, microscopic surface observation, X-ray photoelectron spectroscopy (XPS) analysis and molecular dynamics (MD) simulation. Results indicate that DNA acts as an effective cathodic-type inhibitor that mainly suppresses cathodic reaction by forming anchored DNA-adsorption film. The film shows active blocking effect, obeys the Langmuir adsorption model and involves physisorption and chemisorption . The MD simulation gives the new insights at molecular level and indicates the formation of an interesting double barrier of DNA molecule on the Cu (111) surface due to its unique twist structure.
研究领域:
材料科学
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电化学
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表面科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）