MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xinzong Li,Hongfeng Gao,Yujun Li,Yonggang Wang,Haiyong Zhang
A novel type of humic acid-based nitrogen-doped graphene quantum dots (N-GQDs) exhibiting strong blue fluorescence emission was synthesized using a straightforward hydrothermal method, with coal-derived humic acid and ethanolamine serving as precursors. It was observed that the incorporation of Fe 3+ ions quenches the fluorescence emission of the N-GQDs, the classification of quenching is recognized as static quenching; however, the addition of l -lysine to the N-GQDs/Fe 3+ system effectively restores this fluorescence. Building on these findings, this study further investigates the potential application of N-GQDs as an “on-off-on” fluorescent sensor for detecting both Fe 3+ and l -lysine. It has been established that N-GQDs exhibit commendable selectivity and sensitivity towards Fe 3+ , achieving a detection limit (LoD) of 0.41 μM. Furthermore, the stable N-GQDs/Fe 3+ complex demonstrates significant detection sensitivity to l -lysine in aqueous environments, with a LoD of 1.06 μM. The experimental results indicate that the synthesized N-GQDs possess favorable properties including excellent fluorescence intensity, dispersibility, and water solubility. The developed sensor exhibits considerable potential for improvement and holds promising applications in the domain of water quality monitoring. Additionally, the fluorescence selectivity of N-GQDs towards Fe 3+ was elucidated through calculations involving frontier molecular orbitals.
研究领域:
分析化学
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环境监测
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纳米材料
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生物传感
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水处理
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分子探针
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荧光分析
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产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yuting Wang,Ying Xiong,Hui Tian,Shi Wang,Qianying Zhang,Mingjing Zhang,Hong Ding,Ye Gu,Anlin Peng
A novel disposable sensor modified with MWNTs/Al 2 O 3 /poly-L-lysine film was developed for detection of clinical 17β-estradiol (17β-E 2 ) urine samples in this study. The electrochemical behavior of 17β-E 2 was greatly increased due to the good electrocatalytic activity of MWNTs/Al 2 O 3 /poly-L-lysine nanocomposites. The optimum experimental conditions such as scan rate, pH, accumulation time were obtained, and the peak potentials of 17β-E2 was explored at about 530mV. The peak current of 17β-E 2 measured by CV displayed the good linear property with the increasing of the concentrations. Under optimized conditions, the disposable sensor displayed excellent analytical performance with linear ranges of 17β-E 2 from 0.5nM to 50nM. And a very low detection limit of 0.014 nM was attained. Furthermore, the MWNTs/Al 2 O 3 /poly-L-lysine SPE was used to the rapid and successful determination of the hormone in spiked synthetic and real clinical pregnant woman urine samples with wonderful selectivity and sensitivity.
研究领域:
生物医学工程
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临床检验医学
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分子诊断学
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电化学分析
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纳米生物技术
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激素检测
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Chongchong Miao,Amin Zhang,Yanan Xu,Shen Chen,Feimeng Ma,Chusen Huang,Nengqin Jia
A new electrochemiluminescence (ECL) sensor was constructed for the ultrasensitive detection of diphenhydramine hydrochloride (DPH) based on l -cysteine-functionalized multiwalled carbon nanotubes/gold nanoparticles (MWCNTs-Cys-AuNPs) nanocomposites. In this work, the nanocomposites were facilely synthesized by using l -cysteine as linking agent which could covalently conjugate to MWCNTs and AuNPs via amide bond and Au S bond. Herein, the prepared MWCNTs-Cys-AuNPs were used as matrix material and fixed by Nafion to adsorb Ru(bpy) 3 2+ on glassy carbon electrode (GCE) to provide a new ECL sensor for DPH . Then the electrochemical and ECL behaviors of the sensor were investigated. Approximate 9-fold enhancement in ECL intensity was observed after addition of DPH. By optimizing the experimental conditions, the proposed ECL sensor exhibited excellent assay performances in determination of DPH with a wide linear range (2 × 10 −8 –7.5 × 10 −4 M), low detection limit (6.7 × 10 −9 M), good reproducibility and acceptable stability. Furthermore, the constructed ECL sensor was used to analyze the DPH in real sample with a satisfactory result, which holds a great potential in the field of pharmaceutical analysis.
研究领域:
分析化学
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药物分析
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生物传感器
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纳米医学
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电化学
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DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Lin Yuan,Lizhen Liu,Yunfeng Bai,Jun Qin,Meng Chen,Feng Feng
In this article, we report for the first time the use of a dual-emission carbon dots (CDs) with orange-yellow fluorescence for the detection of l -glutamic acid (L-Glu). The CDs was synthesized through a facile strategy of one-pot hydrothermal route using o -phenylenediamine (oPD) and oxalic acid . The CDs exhibit two fluorescence emission peaks around 453 nm and 560 nm when the excitation wavelength is at 390 nm. In the existence of L-Glu the fluorescence at 560 nm was decreased, whereas the fluorescence at 453 nm was constant. The fluorescence intensity ratio at 560 nm and 453 nm (F 560 /F 453 ) expressed two great linear relationships in the L-Glu concentration range from 0 to 200 μM and 200–400 μM, respectively, with a detection limit (LOD) of about 0.085 μM. In addition, it was used to analyze L-Glu in fetal bovine serum samples successfully, which recoveries were ranging from 97.07 to 103.7%. Those results demonstrate CDs can be further explored in biomedicine studies.
研究领域:
分析化学
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生物医学工程
·
纳米技术
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Yu-Cai He,Dan-Ping Zhang,Zhi-Cheng Tao,Yun Lu,Yun Ding,Feng Liu,Zheng-Zhong Zhu,Huan Rui,Gao-Wei Zheng,Xian Zhang
To reduce dependence on the expensive cofactor and effectively biotransform ethyl 4-chloro-3-oxobutanoate, l -glutamine and glycine were found to enhance the content of intracellular NADH and the reductase activity. Adding the mixture of 200 mM of l -glutamine and 500 mM of glycine to the reaction media, a 1.67-fold of reductase activity was increased over the control without the addition of the two compounds. Moreover, β-cyclodextrin (0.4 mol β-cyclodextrin/mol ethyl 4-chloro-3-oxobutanoate) was also added into this reaction media, and the biocatalytic activity of the whole-cell biocatalyst of Escherichia coli CCZU-K14 was increased by 1.34-fold than that without β-cyclodextrin. In this β-cyclodextrin–water media containing l -glutamine (200 mM) plus glycine (500 mM), ethyl ( S )-4-chloro-3-hydroxybutanoate (>99% ee ) could be obtained from 3000 mM ethyl 4-chloro-3-oxobutanoate in the yield of 98.0% after 8 h. All the positive features demonstrate the potential applicability of the bioprocess for the large-scale production of ethyl ( S )-4-chloro-3-hydroxybutanoate.
研究领域:
生物化学
·
酶工程
·
有机合成
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2020
·
IF 7.539
Lian Xu,Li-Chao Wang,Bing-Mei Su,Xin-Qi Xu,Juan Lin
L-threonine transaldolase (PsLTTA) could asymmetric synthesize β-hydroxy-α-amino acids (HAAs) with excellent stereoselectivity, while the poor yield limited its further application. Here we provided a combinatorial strategy to improve HAAs production, by directed evolution of PsLTTA towards enhanced activity and introducing an acetaldehyde elimination system to avoid acetaldehyde over-accumulation. A novel high throughput screening (HTS) method for evaluating PsLTTA activity was developed and applied for directed evolution of PsLTTA. Subsequently, we co-expressed alcohol dehydrogenase and formate dehydrogenase to construct an acetaldehyde elimination system to remove acetaldehyde inhibition. Moreover, the above positive strategies were integrated. As a result, the (2S,3R)- p -methylsulfonyl phenylserine yield reached 154.0 mM and with 94.6% de value, the highest productivity and stereoselectivity of (2S,3R)-HAAs reported by enzymatic synthesis so far. Taken together, our studies provided an efficient and green route for chiral synthesis of (2S,3R)-HAAs, which might contribute to the industrialization production of these useful building blocks.
研究领域:
生物化学
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生物技术
·
酶工程
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2022
·Vol. 41
·期 7
·1115-1122
·
IF 3.815
Zhong Chan-Na,Liu Ya-Dong,Tang Juan,Chen Wei-Shuo,Li Shuang-Cheng,Shao Jun,Hou Hao-Qing
Blending of poly(levorotatory-lactic acid) (PLLA) and poly(dextrorotatory-lactic acid) (PDLA) produces the stereocomplex crystallites (PLA SC), which present higher melting temperature and mechanical properties than that of neat PLLA or PDLA. However, in the PLLA/PDLA blends with higher molecular weights, the phase separation occurs and the SC exhibits weak memory after melting, which lead to a small amount of SC together with a large amount of homochiral crystallites (HC) develop during crystallization from the melt. In this study, a small content of graphite oxide was blended with PLLA and PDLA to form ternary blends, and it was exciting to find that the formation of SC was enhanced gradually with the content of graphite oxide. The SC exclusively developed when 2 wt% graphite oxide was incorporated into the PLLA/PDLA, and the crystallinity with ∼50% was received even during fast cooling from the melt (−50 °C/min). The acceleration formation of SC was speculated due to the interaction between PLA molecular chains and the hydroxyl groups on the surface of graphite oxide and the obstruction of proliferation of graphite oxide.
研究领域:
高分子科学
·
材料工程
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纳米复合材料
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结晶动力学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2019
·
IF 7.149
Xinghao Wang,Wenrui Xiang,Siyuan Wang,Jiali Ge,Ruijuan Qu,Zunyao Wang
Soluble manganese(III), stabilized by ligands as Mn(III)-L complexes, are ubiquitous in natural waters and wastewaters and can potentially serve as both the oxidant and reductant in one-electron transfer reactions with organic contaminants. In this study, the oxidative transformations of 14 phenolic endocrine disrupting chemicals (EDCs) by in situ-formed Mn(III)-L complexes, generated from irradiated water containing Mn(II) and humic acid, were investigated. The pseudo-first-order rate constants (kobs, h–1) of these phenols varied from 1.0 × 10–4 to 5.9 × 10–2. A quantitative structure–activity relationship model was developed, which suggests that the electron-donating ability (EHOMO) of phenolic chemicals was the most important molecular characteristic for the Mn(III)-L-mediated oxidative transformation. Phenol transformation was initiated by the generation of a phenoxyl radical through electron transfer to Mn(III)-L. Subsequent self-coupling reactions between phenoxyl radicals resulted in the formation of self-coupling dimers and trimers. With the addition of simple phenol as a cosubstrate, enhanced transformations of these phenolic EDCs were clearly observed, and cross-coupling products of simple phenol and the substrates were also detected. In addition, a reaction activation energy calculation based on the transition-state theory indicated that the cross-coupling reaction was more likely than the self-coupling reaction to occur in the presence of phenol. This work provides new insights into the environmental fate of phenolic compounds.
研究领域:
环境科学
·
化学
·
生物学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 16
·2800
·
IF 5.1
Yangzu Gao,Haoyu Li,Qian Wu,Bang Chen,Kangzhen Xu,Cong Li,Yehua Shen
The functional exploration of natural foods, coupled with the increasing prevalence of gastrointestinal motility disorders and the associated therapeutic challenges, has generated significant interest in this field. This study aims to investigate the ameliorative effects of the extract fromPugionium cornutum(L.) Gaertn (EAEPC), a traditional edible vegetable in northwest China’s desert region, on atropine-induced gastroparesis in mice, as well as to elucidate its mechanism in terms of the gut microbiota and major metabolites. The findings indicate that EAEPC effectively reduces the rate of pigment residual in the stomach while shortening the gastrointestinal transit time and alleviating other symptoms associated with atropine-induced gastroparesis. These effects may be mediated through modulation of the expression levels of major intestinal metabolites, such as short-chain fatty acids (SCFAs), bile acids (BAs), and L-tryptophan, alongside remodeling of both the diversity and relative abundance of the gut microbiota. Furthermore, correlation analyses were conducted on significantly altered strains and metabolites to clarify their interactions. Moreover, the chemical constituents of EAEPC were identified by UPLC-Q-TOF-MS/MS, and the key active components responsible for improving gastroparesis were predicted through network pharmacology approaches and validated experimentally. These results provide a foundation for further research into the functions ofPugioniumand offer scientific support for developing natural plant-based strategies aimed at treating gastrointestinal motility disorders.
研究领域:
消化病学
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天然产物药理学
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肠道微生态学
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代谢组学
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分子药理学
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植物医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2014
·
IF 7.261
Haiyang Yu,Zhaohui Tang,Dawei Zhang,Wantong Song,Ying Zhang,Yan Yang,Zaheer Ahmad,Xuesi Chen
Platinum-based polymeric nano-drugs, especially cisplatin-loaded polymeric nanoparticles (CDDP-NPs), have been extensively exploited for the treatment of solid tumors . However, it is still unclear what role the processing procedure and the properties of the polymeric carrier materials may play in influencing the plasma pharmacokinetics , biodistribution and in vivo efficacy of CDDP-NPs. In this study, a series of poly( l -glutamic acid)- g -methoxy poly(ethylene glycol) (PLG- g -mPEG) copolymers were synthesized for the preparation of CDDP-loaded PLG- g -mPEG (CDDP/PLG- g -mPEG) nanoparticles . All of the parameters, including PLG molecular weight, mPEG/PLG weight ratio, mPEG chain length, ultrafiltration purification and cisplatin loading content, were found to have a significant influence on the plasma pharmacokinetics of the CDDP/PLG- g -mPEG nanoparticles . The blood circulation time of the nanoparticles was prolonged with increases in PLG molecular weight, mPEG/PLG weight ratio, mPEG chain length and CDDP loading content. The use of ultrafiltration purification could prolong the blood circulation time of the nanoparticles as well. Experiments to measure the pharmacokinetics and biodistribution demonstrated that the selected CDDP/PLG- g -mPEG nanoparticles, NP10, had a long blood circulation time and could achieve selective and significant accumulation in Lewis lung carcinoma (LLC) tumors. The platinum plasma concentrations in the LLC tumor-bearing mice receiving NP10 remained up to 46-fold higher than that of mice receiving equivalent doses of free CDDP. In addition, the plasma area under the concentration time curve (AUC) of NP10 was 31-fold higher than that of free CDDP in 48 h. The platinum concentration ratio of NP10 to free CDDP in tumors reached as high as 9.4. The tumor AUC ratio of NP10 to CDDP was 6. Using a mouse C26 tumor model, here we demonstrate that NP10 improves the safety and tolerance in vivo when compared to CDDP and effectively inhibits the growth of C26 tumors. Furthermore, increasing the dosage of NP10 by 2 or 3-fold of free CCDP improved its anticancer efficacy to comparable or higher levels. These results indicate that CDDP/PLG- g -mPEG nanoparticles have greater potential for the treatment of solid tumors in clinical application.
研究领域:
药理学
·
肿瘤学
·
纳米医学
✓ 已验证
DOI
高影响因子
Materials Today Communications
·
2025
·
IF 4.5
Xiaodong Wang,Qinghui Luo,Chunyu Zhang,Shuanglin Gong,Zihao Qin,Zhibo Yu,Yujie Liu,Shilong He,Tingting Cao
To address escalating waterborne heavy metal contamination and low utilization of agricultural waste, this study developed L-arginine-functionalized corn straw biochar (Arg-CSB) via hydrothermal treatment, leveraging the amino and carboxyl groups of arginine for surface modification. Multi-technique characterization revealed that Arg- CSB retained the native fibrous carbon skeleton of corn straw while enriching surface functional groups—notably carboxyl groups and guanidinium moieties. Adsorption experiments demonstrate selective Pb(II) binding by Arg-CSB, achieving 83.4 % removal efficiency at pH 6 and 20°C, which is double that of unmodified biochar (CSB). Kinetic analysis followed the pseudo-first-order model, indicating predominant chemical adsorption, while the Langmuir isotherm confirmed monolayer adsorption behavior with a theoretical maximum capacity of 33.29 mg/g. Furthermore, Arg-CSB demonstrated excellent stability, maintaining a 69.9 % Pb(II) removal rate after the fifth cycle experiment. This work presents a green, efficient strategy for upgrading agricultural waste into functional adsorbents, offering high-value resource recycling and targeted heavy metal remediation.
研究领域:
环境科学
·
材料工程
·
化学工程
·
水处理技术
·
农业废弃物管理
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产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Yangzhou Xie,Sitao Wang,Yu Tian,Kexi Ye,Zhigang Xu,Han Zheng,Wei Jiang,Fengmei Zhang,Xiaoxi Si
Excessive D-lactic acid in fermented foods can result in unpleasant flavors and is difficult for the human body to metabolize. This study is the first to combine tandem column technology (C18 and Poroshell chiral-T) with a machine learning strategy. The method achieved a resolution of 2.86, with a concentration range of 50.00 μg·L −1 -50.00 mg·L −1 and a limit of detection of 16.67 μg·L −1 . We aimed to classify different lactic acid products and tobacco, and the research showed that L-lactic acid was predominant in all food analyses , while D-lactic acid was only found in lactic acid products In addition, the machine learning algorithms were optimized, and the random forest model effectively distinguished samples with similar lactic acid content. Based on this collaborative model of tandem column and machine learning, lactic acid chiral biomarkers gained the ability to classify food samples. The accuracy of model reached 80 %. This strategy contributes to the chiral analysis of lactic acid in complex food matrices. It also demonstrates the practical value of classification in food research.
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）