PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Haiyan Yu,Tao Zhang,Cunyu Wei,Juan Yang,Xinle Ma,Jingchao Chen,Hailan Cui,Feng Liu,Xiangju Li
BACKGROUND Mesosulfuron-methyl (MSM) is currently the only available selective herbicide for controlling Aegilops tauschii in wheat fields. Improving the control performance of MSM against Ae. tauschii is crucial for ensuring wheat yield. Herein, strategies to improve the bioactivity of MSM against Ae. tauschii and the underlying mechanism were investigated. RESULTS The efficacy of MSM oil dispersion (MSM-OD, GR 50 = 21.62 g a.i. ha −1 ) on Ae. tauschii was higher than emulsion in water (GR 50 = 59.96 g a.i. ha −1 ) and emulsifiable concentrate (GR 50 = 77.34 g a.i. ha −1 ). A greater retention on Ae. tauschii leaves was responsible for the increased MSM-OD bioactivity. As the particle size decreased from 13.58 to 1.20 μm, the herbicidal activity of MSM-OD towards Ae. tauschii increased 3.07-fold, which was attributed to the small particle size-enabled faster plant absorption of MSM. Polyvinyl alcohol (PVA, 3%) addition in MSM-OD with a small particle size (MSM-OD-S + PVA) increased MSM residue on Ae. tauschii leaves after washing, and thus herbicidal activity. The increased wettability on Ae. tauschii leaves was related to the PVA-induced rainfastness increase of MSM-OD-S. Importantly, MSM-OD-S + PVA at the recommended field rate did not show an adverse effect on wheat growth. CONCLUSION A suitable formulation, reduced particle size and PVA addition can increase the herbicidal activity of MSM against Ae. tauschii , particularly under rainy conditions. These results can provide guidance for the development and application of other herbicides. © 2025 Society of Chemical Industry.
研究领域:
农业化学
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杂草科学
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农药制剂
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植物生理学
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作物保护
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·Vol. 80
·期 9
·4253-4263
·
IF 3.8
Ying Zhang,Tao Zhang,Xinhang Wang,Zhipeng Bian,Xiaofang Zhang,Guoqing Yang,Yanhui Lu
BACKGROUND Insects use odor detection to sense their surroundings. Use of volatile compounds, such as essential oils (EOs) of plants, to repel pests and disrupt their olfaction-driven behaviors has great practical potential for use in integrated pest management. Despite the available information on the repellent effects of EOs on herbivorous insects, the olfaction-based mechanisms remain unknown. RESULTS Y-tube olfactometer tests showed that the EOs of three Lamiaceae plants – Mentha arvensis L., Mentha piperita L. and Lavandula angustifolia Mill. – were significantly repellent to winged cotton aphid, Aphis gossypii Glover. Electrical penetration graph (EPG) tests indicated the EOs reduced phloem feeding and increased the level of non-productive probing by the aphids. The EOs also reduced the fecundity of winged Aphis gossypii . Electrophysiological bioassays and gas chromatography–mass spectrometry (GC–MS) identified five physiologically active volatiles, that is menthone, isomenthone, neomenthol and menthol from Mentha piperita ; menthone and menthol from Mentha arvensis ; and linalool from L. angustifolia . Behavioral tests confirmed that all five compounds repelled winged Aphis gossypii . Under field conditions, the growth rate of aphid populations after 7 days was significantly lower in fields treated with these compounds than in the control fields. CONCLUSION Our findings demonstrated that three EOs not only repelled winged Aphis gossypii but also interfered with the aphid's feeding behavior and reduced its fecundity. These EOs and their active constituents have great potential as eco-friendly control products for use against Aphis gossypii . The effects of these EOs also exceed other repellents that only keep pests away from host plants. © 2024 Society of Chemical Industry.
研究领域:
植物学
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昆虫学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
He Wu,Wen-Hao Han,Kai-Lu Liang,Jun-Xia Wang,Feng-Bin Zhang,Shun-Xia Ji,Shu-Sheng Liu,Xiao-Wei Wang
BACKGROUND Jasmonic acid (JA) is an important phytohormone used to defend against herbivores, but it does not respond to whitefly feeding. Conversely, another phytohormone, salicylic acid (SA), is induced when plants are fed upon by whiteflies. JA has a better anti-whitefly effect than SA; however, there is limited research on how to effectively improve plant resistance by utilizing the different responses of these phytohormones to whitefly feeding. RESULTS We discovered that protease inhibitors 8 ( PI8 ) and terpene synthase 10 ( TPS10 ) located downstream of the JA-regulated pathway in plants have anti-whitefly effects, but these two genes were not induced by whitefly feeding. To identify whitefly-inducible promoters, we compared the transcriptome data of tobacco fed upon by Bemisia tabaci with the control. We focused on pathogenesis - related ( PR ) genes because they are known to be induced by SA. Among these PR genes, we found that expression levels of pathogenes - related protein 1C - like ( PR1 ) and glucose endo - 1 , 3 - beta - glucosidase ( BGL ) can be significantly induced by whitefly feeding and regulated by SA. We then engineered the whitefly-inducible promoters of BGL and PR1 to drive the expression of PI8 and TPS10 . We found that compared with control plants that did not induce the expression of PI8 or TPS10 , transformed plants expressing PI8 or TPS10 under the PR1 or BGL promoter showed a significant increase in the expression levels of PI8 and TPS10 after whitefly infection, significantly improving their resistance to whiteflies. CONCLUSION Our findings suggest that using SA-inducible promoters as tools to drive the expression of JA-regulated defense genes can enhance plant resistance to whiteflies. Our study provides a novel pathway for the enhancement of plant resistance against insects. © 2024 Society of Chemical Industry.
研究领域:
植物学
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分子生物学
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昆虫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Tao Sun,Jiangbo Song,Jingzhi Luo,Yu Jiang,Duan Tan,Lei Zhou,Wentao Wu,Minjin Han,Hai Hu,Xiaoling Tong,Cheng Lu,Fangyin Dai
BACKGROUND Diverse lepidopteran insects cause serious damage to plants, and their larvae possess a crucial epidermal barrier against environmental stimuli. Their ultraviolet (UV) resistance is enhanced by accumulating uric acid granules in the epidermis, suggesting that genes involved in this process may be potential targets for lepidopteran pest management. RESULTS The silkworm pan-genome dataset is a valuable source for studying genomic mutations and phenotype–genotype associations. Hoarfrost translucent ( oh ) is a recessive silkworm mutant with a translucent larval integument. Using comparative genomic analysis, we found that the oh mutant has an 828-bp deletion in the BmBLOC1S6 genome. BmBLOC1S6 encodes a BLOC-1 complex subunit and is conserved during lepidopteran evolution. Knockout of BmBLOC1S6 replicated the oh phenotype. Furthermore, BmBLOC1S6 knockout and oh larvae are more sensitive to UV irradiation compared to the wild-type. These results revealed that BmBLOC1S6 is essential in forming uric acid granules for silkworm epidermal UV resistance. CONCLUSION Our results showed that BmBLOC1S6 is responsible for the oh phenotype in silkworms and is conserved during lepidopteran evolution. This study may help us better clarify uric acid granules formation in the epidermis, explore their function in UV resistance and identify a potential molecular target for lepidopteran pest control. © 2025 Society of Chemical Industry.
研究领域:
昆虫学
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遗传学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Yuecheng Zhao,Lu Zhang,Chuanshan Zou,Huilin Han,Chengde Li,Xingpeng Li,Liwen Song
BACKGROUND Cuticular proteins (CPs) play essential roles in forming cuticular structures in insects. However, the specific functions and regulatory mechanisms of CPs remain largely unexplored. In this study, the Larval cuticular protein 17 ( HcLCP-17 ) gene was identified from Hyphantria cunea , a highly destructive and polyphagous forest pest. To investigate the role of HcLCP-17 in cuticular function and transcriptional regulation mediated by 20E-responsive transcription factors (ERTFs), we employed RNA interference (RNAi) and yeast one-hybrid assay techniques. Additionally, we examined the molecular mechanism by which chlorbenzuron, a type of benzoylphenylurea (BPU) that functions as a chitin synthesis inhibitor (CSI), affects the 20E signaling pathway and ultimately regulates HcLCP-17 expression. RESULTS HcLCP-17 encodes a polypeptide consisting of 393 amino acids, which includes a chitin-binding domain. Silencing HcLCP-17 resulted in a disturbance in the structural organization of the larval cuticle and a notable reduction in chitin levels. HcLCP-17 expression was controlled by the interaction between Broad-Complex (Br-C) and beta Fushi Tarazu Factor-1 (βFTZ-F1) with its promoter fragment. Furthermore, the inhibitory effect of chlorbenzuron on HcLCP-17 expression was found to be potentially mediated by Br-C and βFTZ-F1. CONCLUSION The study presents a novel mode of action for the 20E signaling pathway in regulating the expression of CPs and reveals the potential mode-of-action of BPUs in insect cuticles. These findings provide a theoretical basis for future utilization of LCP-17 as a pesticide target making a significant contribution to the development of effective pest management strategies. © 2024 Society of Chemical Industry.
研究领域:
昆虫学
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分子生物学
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害虫管理
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Yun Wang,Hui-Qian Xu,Hong-Liang Han,Dong Chen,Hongbo Jiang,Guy Smagghe,Jin-Jun Wang,Dong Wei
BACKGROUND The melon fly, Zeugodacus cucurbitae (Coquillett), is an invasive Tephritidae pest with robust fertility. The male accessory glands (MAGs) form a vital organ that ensures insect reproductive efficiency. Most of the secreted proteins by MAGs exhibit a male bias expression. Takeout, one of these proteins, is abundantly present in the MAGs of many insects. RESULTS In this study, we identified 32 takeout genes in Z. cucurbitae . The phylogenetic analysis and multiple sequence alignment results showed that Zctakeout1 is the most related homolog to the MAGs-specific takeout in Tephritidae. The real-time quantitative PCR results showed that Zctakeout1 was exclusively expressed in the male adult stage, and its expression level gradually increased with the increase in age and then remained stable at the sexually matured stage. The distribution among tissues demonstrated the specific expression of Zctakeout1 in the MAGs, and fluorescence immunohistochemical results confirmed the presence of Zctakeout1 in close proximity to binuclear cells of the mesoderm epidermal MAGs. In continuation, CRISPR/Cas9-mediated genome editing was employed, resulting in successfully generating a homozygous strain with an +8 bp insertion. The mating experiments with the Zctakeout1 −/− males resulted in significant reductions in both the mating rate and egg production of females. CONCLUSION These findings prove that the MAGs-specific Zctakeout1 is essential in regulating fecundity in female Z. cucurbitae fruit flies. Our data suggests its utilization in future essential insect-specific gene-directed sterility insect technique (SIT) by the genetic manipulation to keep these important Tephritidae populations under control. © 2024 Society of Chemical Industry.
研究领域:
昆虫学
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遗传学
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分子生物学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Xing Chen,Junpeng Yang,Lingmin Xia,Rui Yang,Chaoyang Ding,Xueping Huang,Yu Chen,Jian Luo
BACKGROUND At present, it is vital to develop a stable and efficient pesticide delivery system to optimize pesticide foliar utilization, which could improve control efficacy, enhance resistance to adverse climates, and prolong foliar retention. In this study, reaction monomers methylene diphenyl diisocyanate (MDI) and polycaprolactone diol (PCL) were used to synthesize a polymer network structure for loading the organic phase of pesticides in a micron-reactor, then the shell was formed by sodium lignosulfonate (SL) and didecyl dimethyl ammonium chloride (DDAC) through electrostatic self-assembly, resulting in self-assembled microcapsules and efficient pesticide loading, and the stability and efficacy were discussed. RESULTS Self-assembled microcapsules Pyr@MCs-C and Pyr@MCs-V with cores of different mechanical strength and morphological characteristics are realized by regulating the reaction ratio of MDI and PCL. Compared with conventional self-assembled microcapsule Pyr@MCs-S, Pyr@MCs-C and Pyr@MCs-V exhibit stable and unruptured morphology in dehydrated environment. Moreover, self-assembled microcapsules provide similar fungicidal activity as emulsifiable concentrate. Notably, the washout resistance property of Pyr@MCs-C and Pyr@MCs-V increased by 3.20 and 3.51 times, respectively, and ultraviolet (UV) resistances of the two microcapsules increased by 5.72 and 5.02 times, respectively, which promote the control efficiency and prolong the duration. CONCLUSION In summary, this system has simple preparation process and stable foliar performances, making it a promising precise pesticide delivery platform. © 2025 Society of Chemical Industry.
研究领域:
农业科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Shuning Chen,Ao Yuan,Huafeng Wang,Daibin Yang,Li Cui,Fengjiao Hao,Xiaojing Yan,Huizhu Yuan
BACKGROUND Citrus huanglongbing (HLB) is a devastating disease in citrus, caused by Candidatus Liberibacter asiaticus (CLas), which primarily resides in the phloem where chemicals cannot effectively reach, posing a significant challenge in controlling HLB. To address these challenges, plant essential oils (EOs), widely used as transdermal enhancers and known for their benefits for plant tissues, were investigated for their potential to enhance chemical permeation. RESULTS In this study, seven EOs – eugenol, carvacrol, eucalyptol, geraniol, linalool, cinnamaldehyde, and d -limonene – were evaluated for their potential to enhance chemical penetration into citrus leaves. Preliminary screening with tracer methods revealed that geraniol provided the greatest enhancement of permeation, achieving the deepest and broadest distribution into the spongy tissue of the citrus leaf. The permeation levels of rhodamine B and crystal violet through the cuticular layer were elevated from 0.55 mg L −1 and 0.11 mg L −1 to 20.04 mg L −1 and 7.14 mg L −1 , respectively, by the addition of geraniol. Further validation through high-performance liquid chromatography–mass spectrometry (HPLC–MS) confirmed that the penetration of fludioxonil and tetracycline into citrus leaf tissues was enhanced by 15.07-fold and 20.43-fold, respectively. In planta experiments confirmed that adding geraniol to tetracycline and oxytetracycline via foliar application eliminated CLas bacteria in HLB-affected citrus more efficiently than controls. CONCLUSION Our study underscores the potential of geraniol as an effective EO-based permeation enhancer for combating HLB. By significantly improving the delivery and efficacy of bactericidal treatments, geraniol offers a promising strategy for managing this catastrophic citrus disease, potentially transforming the approach to controlling HLB. © 2024 Society of Chemical Industry.
研究领域:
植物病理学
·
农业化学
·
植物化学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Hui-Ming Xiang,Xiao-Hong Wei,Yi-Ran Li,Bo-Jun Zhang,Min Li,Rui-Yan Ma
BACKGROUND As sex pheromones are environmentally friendly and specific, they are often used to monitor and control oriental fruit moths (OFMs). Currently, non-biodegradable polymers are commonly employed as carriers to prepare controlled sex pheromone release systems for plant protection. Electrospinning is a relatively simple technique for preparing biodegradable nanofibers that allows for the controlled release of sex pheromones. This study aimed to develop a biodegradable controlled release system for the combination of OFM pheromone and codlemone via electrospinning, in which codlemone was used as a synergist for OFM pheromone. RESULTS New systems for the controlled release of combined pheromones from OFM and colding moths were developed using electrospun nanofibers from polycaprolactone (PCL), cellulose acetate (CA), and polylactic acid (PLA). In the indoor experiments, the load ratio and release stability of CA nanofibers loaded with combined pheromones (CA-SP) were superior to those of the other two nanofiber types. In the field experiments, among all the treatments, 10 mg of CA-SP was the most attractive to OFMs, and its effective duration was approximately 6 weeks. The optimum storage temperature for all nanofibers was −20 °C. CONCLUSION Electrospun CA-SP nanofibers were biodegradable and environmentally friendly, with stable release. This study presents a new technique that could be beneficial for the monitoring and control of OFMs. © 2025 Society of Chemical Industry.
研究领域:
昆虫学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Yanling Wu,Songhao Zhang,Zhenping Bao,Rui Liu,Fengzhe Sun,Yue Wang,Haiqi Huang,Yuxia Gao,Yongqiang Ma,Chenhui Zhang,Fengpei Du
BACKGROUND Biotic and abiotic stresses threaten crop growth and yield. Agrochemicals are an important way to mitigate biotic stress, while frequent low utilization and potential environmental risk affect their sustainable use. In order to improve pesticide utilization, it is common practice to add tank-mix adjuvants by reducing surface tension or forming spherical self-assembly. However, there is a lack of quantitative indicators to screen suitable molecules for sustainable application. In this work, critical factors based on physicochemical properties, and kinetic and thermodynamic parameters are applied to analyze regulatory mechanisms in dynamic processes, and ultimately to establish an integrated strategy for the management of stresses. RESULTS Compared with traditional one-dimensional linear amphiphilic molecules, two-dimensional bio-based amphiphilic molecules, especially sodium deoxycholate (NaDC), form self-assembly and could significantly promote the deposition of agrochemical droplets due to maximum energy dissipation. Meanwhile, NaDC increased the inhibition rate of pyraclostrobin against Rhizoctonia solani from 24.4% to about 100.0%, which was beneficial for pesticide resistance to biotic stress. In addition, NaDC could significantly mitigate the harmful effects of salt stress on Oryza sativa by increasing the germination rate of salt-stressed seeds by about 30%, and reducing the environmental risk of pesticides to soil microbial communities for eco-friendly crop protection. CONCLUSION Herein, this work demonstrates a sustainable strategy for crop management that enhances the effects of agrochemicals on biotic stresses, mitigates abiotic stresses, and significantly reduces environmental risks. © 2025 Society of Chemical Industry.
研究领域:
农业科学
·
环境科学
·
植物保护
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产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·Vol. 80
·期 12
·6566-6574
·
IF 3.8
Mengwei Zhang,Xingyu Ren,Yuying Li,Yaqiang Wang,Yi Li,Zhiqing Ma,Yong Wang,Juntao Feng
BACKGROUND Sclerotinia sclerotiorum , a pathogenic fungus of oilseed rape, poses a severe threat to the oilseed rapeseed industry. In this study, we evaluated the potential of the natural compound hinokitiol against S. sclerotiorum by determining its biological activity and physiological characteristics. RESULTS Our results showed that hinokitiol strongly inhibited the hyphae expansion of S. sclerotiorum , and its effective concentration of hyphae growing inhibition by 50% (EC 50 ) against 103 S. sclerotiorum strains varied from 0.36 to 3.45 μg/mL, with an average of 1.23 μg/mL. Hinokitiol possessed better protective efficacy than therapeutic effects, and it exhibited no cross-resistance between carbendazim. After treatment with hinokitiol, many vesicular protrusions developed on the mycelium with rough surface and thickened cell wall. Moreover, the cell membrane permeability and glycerol content increased, while the oxalic acid declined after hinokitiol treatment. In addition, hinokitiol induced membrane lipid peroxidation and improved the production of reactive oxygen species (ROS) in S. sclerotiorum . Importantly, real-time quantitative polymerase chain reaction showed that cell wall and ROS synthesis-related genes were significantly up-regulated after hinokitiol treatment. CONCLUSION This study revealed that hinokitiol has good biological activity against S. sclerotiorum and could be considered as an alternative bio-fungicide for the resistance management in controlling sclerotinia stem rot infected by S. sclerotiorum . These investigations provided new insights into understanding the toxic action of hinokitiol against pathogenic fungi. © 2024 Society of Chemical Industry.
研究领域:
植物保护
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微生物学
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植物病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Min Tan,Yanxia Fang,Yuanhe Zhang,Sheng Qiang
BACKGROUND Microstegium vimineum (Trin.) A. Camus, commonly called stiltgrass, is a dominant weed in the United States and China. Although a lot of control approaches have been attempted, an economic, effective and practical measure has not been available to control the weed so far. RESULTS A serious rust disease of Microstegium vimineum was observed in three regions of Wenzhou city in China, from 2019 to 2021, with a disease incidence ranging from 82% to 97%. Typical rust disease symptoms on Microstegium vimineum were prominently visible during the early monsoon season (June–July), with chlorotic spots on the leaf surfaces. The morphological characterization of the strain WZ-1 which was isolated from the diseased leaves was consistent with Puccinia polliniicola . The virulence tests showed that the average disease index of Microstegium vimineum plants could reach 35% at 10 days post-inoculation. The host specificity of Puccinia polliniicola was tested on 64 plant species from 12 families and it did not cause any diseased symptoms on 24 major crops and 36 weeds, but slightly infected four gramineous weeds, Arthraxon hispidus , Polypogon fugax , Cynodon dactylon , and Microstegium ciliatum . However, newly-produced urediniospores were not observed on the slightly infected plants. The urediniospores of strain WZ-1 infected the Microstegium vimineum leaves by two main approaches: mycelium or appressorium invaded the stoma; and mycelium or appressorium directly invaded intercellular spaces. Field experiments showed that the rust disease naturally prevailed among Microstegium vimineum populations, causing severe rust disease symptoms on the leaf surface. The rust epidemic effectively controlled all of the target plants in the closed plot where the rust was released. CONCLUSION Puccinia polliniicola strain WZ-1 has great potential to be used as a classical biological control agent against Microstegium vimineum . © 2024 Society of Chemical Industry.
研究领域:
植物病理学
·
杂草科学
·
生物控制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）