MICROCHIMICA ACTA
·
2023
·Vol. 191
·期 1
·1-8
·
IF 5.7
Che Dou,Cao Xitao,Chen Chong,Yan Hui
The rapid detection of Pseudomonas aeruginosa ( P. aeruginosa ) is of great significance for the diagnosis of medical infection. In view of the above, a novel aptasensor based on fluorescence resonance energy transfer (FRET) was developed. It contained aptamer-coupled upconversion nanoparticles (UCNPs-apt) as a donor (excitation 980 nm) and molybdenum disulfide (MoS 2 ) nanosheets as an acceptor. The upconversion fluorescence aptamer system was investigated to obtain the optimal parameters of MoS 2 concentration, the incubation time of UCNPs-apt/MoS 2 and P. aeruginosa , and pH. Based on the optimal parameters, a linear calibration equation (emission 654 nm) with a wide detection range 8.7 × 10 ~ 8.7 × 10 7 cfu/mL, a high coefficient of determination R 2 0.9941, and a low limit of determination (LOD) 15.5 cfu/mL were established. The method was validated with P. aeruginosa infected foci of mouse wound. The advantage of this aptasensor is that analysis results can be obtained within 1.5 h, which was much faster than that of the standard method (18–24 h). Furthermore, combined with a portable instrument, it can be used as a point-of-care testing for the early detection of P. aeruginosa infection, which is useful for selecting the correct antibiotics to achieve good therapeutic effects. Additionally, it also has a broad application prospect in food and environmental areas. Graphical abstract
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 30
·期 10
·28227-28237
·
IF 5.19
Liu Huan-Huan,Yang Lei,Guo Lin-Kai,Tu Li-Xin,Li Xiao-Tong,Wang Jia,Ren Yong-Xiang
The occurrence of metal oxide nanoparticles (NPs) in wastewater treatment plants (WWTPs) has raised great concerns about their adverse impacts on nitrification performance. In this study, a heterotrophic nitrifying bacterium Pseudomonas putida strain NP5 showed strong resistance against TiO 2 and NiO NPs. Under 5–50 mg/L NP stress, cell viability was still normal, and the final nutrient removal rates, always higher than 80%, were slightly inhibited. Correspondingly, the PO 4 3− -P removal rates were almost the same as those observed in the control test. Although the enzyme assay demonstrated ammonia monooxygenase and hydroxylamine oxidoreductase activities markedly decreased caused by increased reactive oxygen species (ROS) level under 50 mg/L NPs stress. The total antioxidant capability of NP5 could eliminate excess ROS to maintain a balance between oxidants and antioxidants. Besides, in response to the escalating burden of NPs, strain NP5 tended to secrete more extracellular polymeric substances (EPS), which could protect cell from being damaged by binding to ions and coating. Thus, the strong NP resistance of NP5 would help to overcome the vulnerability of the nitrification process in WWTPs.
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JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2024
·
IF 8
Qi Li,Xiaolei Yang,Changning Li,Aolei He,Shanmu He,Xuemei Li,Ying Zhang,Tuo Yao
Phosphate materials (PMs) combine with phosphate solubilizing bacteria play an essential roles in lead (Pb) immobilization, but their resulting ability to reduce Pb bioavailability may vary depending on PMs used. In this study, Pseudomonas edaphica GAU-665 and three PMs: tricalcium phosphate, calcium phytate and nano-hydroxyapatite were respectively encapsulated into bio-beads by sodium alginate, which immobilization efficiency of Pb 2+ were 99.11%, 97.76% and 99.02% at initial Pb 2+ concentration of 200 mg L −1 , respectively. The Pb 2+ immobilization performance of bio-beads under different conditions and their organic acids secreted were examined. Most Pb 2+ was immobilized by bio-beads through combined functions of adsorption, precipitation, ion exchange and biomineralization, accompanied by the formation of more stable compounds such as Pb 3 (PO 4 ) 2 , Pb 5 (PO 4 ) 3 OH and Pb 5 (PO 4 ) 3 Cl. Meanwhile, pot experimental results indicated that the inoculation of CPhy (calcium phytate) bio-beads with PSB have highest biomass and root growth of oat ( Avena sativa L.) in Pb-stressed compared with CK, which increased the content of chlorophyll b (167.51%) in shoot. In addition, the CPhy bio-beads enhance the peroxidase, catalase activities and reduce the malondialdehyde content to alleviating lead physiological toxicity in oat, which reductions the Pb accumulation in shoot (52.06%) and root (81.04%), and increased the residual fraction of Pb by 165.80% in soil. These findings suggest the bio-beads combined with P. edaphica GAU-665 and calcium phytate is an efficient Pb immobilization material and provided feasible way to improve safety agricultural production and Pb-contaminated soil remediation.
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Journal of Environmental Chemical Engineering
·
2025
·Vol. 14
·期 1
·120844
·
IF 7.2
Xinyu Zhang,Yujie Liu,Ziling Yuan,Haomin Chen,Danyang Ma,Bingzhi Li,Ruihua Liu,Chao Yang
In this study, to remediate 1,2-dichloroethane (DCA)- and 2,4-dichlorophenoxyacetic acid (2,4-D)-polluted wastewater, an autofluorescent engineered strain KTU-DDGV was constructed from a biosafety strain Pseudomonas putida KT2440 by the implantation of the DCA and 2,4-D mineralization pathways, green fluorescent protein (GFP) and Vitreoscilla hemoglobin (VHb). All exogenous genes stably existed in passage cultures of strain KTU-DDGV. The successful transcription of all fourteen exogenous genes in strain KTU-DDGV was confirmed by RT-PCR. Chromatographic analysis demonstrated that strain KTU-DDGV was able to degrade DCA, 2,4-D and their intermediates. The capacity of strain KTU-DDGV to mineralize DCA and 2,4-D was further confirmed by stable isotope assay. When strain KTU-DDGV was incubated with 13 C-labeled DCA and 2,4-D, the 13 C-labeled CO 2 was detected in the collected gases using an isotope mass spectrometer, demonstrating that strain KTU-DDGV was capable of converting 13 C 2 -DCA and 13 C 6 -2,4-D into 13 CO 2 , cell biomass and cellular components. Under oxygen-limited conditions, strain KTU-DDGV still possessed the capability to co-degrade DCA and 2,4-D. The strain KTU-DDGV exhibits visible green fluorescence with naked eyes under daylight, enabling real-time monitoring of strain KTU-DDGV in polluted environments. During wastewater remediation, strain KTU-DDGV maintained high viability and showed high mineralization efficiency for DCA and 2,4-D. This study highlights the potential of strain KTU-DDGV for in situ bioremediation of DCA- and 2,4- D -polluted wastewater. More importantly, constructing composite degradation circuits in a robust chassis may serve as a useful strategy to cope with multiple organic pollutants.
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iScience
·
2022
·
IF 6.107
Fanyang Lv,Yuhua Zhan,Wei Lu,Xiubin Ke,Yahui Shao,Yiyuan Ma,Juan Zheng,Zhimin Yang,Shanshan Jiang,Liguo Shang,Yao Ma,Lei Cheng,Claudine Elmerich,Yongliang Yan,Min Lin
Summary Bacteria of the genus Pseudomonas consume preferred carbon substrates in nearly reverse order to that of enterobacteria, and this process is controlled by RNA-binding translational repressors and regulatory ncRNA antagonists. However, their roles in microbe-plant interactions and the underlying mechanisms remain uncertain. Here we show that root-associated diazotrophic Pseudomonas stutzeri A1501 preferentially catabolizes succinate, followed by the less favorable substrate citrate, and ultimately glucose. Furthermore, the Hfq/Crc/CrcZY regulatory system orchestrates this preference and contributes to optimal nitrogenase activity and efficient root colonization. Hfq has a central role in this regulatory network through different mechanisms of action, including repressing the translation of substrate-specific catabolic genes, activating the nitrogenase gene nifH posttranscriptionally, and exerting a positive effect on the transcription of an exopolysaccharide gene cluster. Our results illustrate an Hfq-mediated mechanism linking carbon metabolism to nitrogen fixation and root colonization, which may confer rhizobacteria competitive advantages in rhizosphere environments.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Ting Liu,Jiamu Kang,Liu Liu
Pseudomonas aeruginosa is a notoriously difficult pathogen to control with antibiotics and disinfectants. Thymus vulgaris L. essential oil (TEO) and thymol are useful for replacing synthetic bacteriostatic agents due to their broad-spectrum bactericidal properties. This study focuses on understanding the inhibitory effects of TEO and its major component against P. aeruginosa , and exploring the antibacterial mechanism from the perspectives of cell membrane destruction, DNA intercalation and biofilm prevention. Results indicate that thymol is the principal component in TEO, as confirmed by GC-MS analysis. TEO and thymol potently combat the planktonic P. aeruginosa and directly change the cell structure. P. aeruginosa cell membrane integrity is destroyed as evidenced by an increase in permeability of the inner and outer membranes and the leakage of intracellular biological macromolecules (DNA). Our study further confirms that the accumulation of intracellular ROS caused by TEO and thymol can result in oxidative stress-mediated DNA damage. Thymol intercalates in the P. aeruginosa genomic DNA to affect the normal function of DNA and even cause bacterial death. Moreover, TEO and thymol affect the biofilm formation and limit the bacterial swimming and swarming motilities. This study provides a potential approach for the development and utilization of natural essential oils and bioactive compunds as novel food additives in the food processing.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Liu Su,Ping Li,Ganggang Zhang,Fengjuan Chen,Daofeng Liu,Weihua He,Cong Liu,Xiaocui Lai,Weihua Lai,Shengliang Deng
Pseudomonas aeruginosa , a hazardous pathogen, poses a significant public health risk through its potential contamination of drinking water. Current culture-based detection methods remain limited by their prolonged duration and operational complexity. In this study, ultrabright orange-emitting aggregation-induced emission nanodots (AIENDs) were successfully prepared through a self-assembly approach constructing a metal-polyphenol network formed by coordination between tannic acid and Zr 4+ . The synthesized ultrabright orange-emitting AIENDs demonstrated excellent biocompatibility and enabled direct conjugation with anti- P. aeruginosa monoclonal antibodies (mAbs) without the need for coupling reagents, enabling rapid antibody immobilization with a coupling efficiency exceeding 94 %. The resulting fluorescent probe (AIEND@mAbs) was employed as an amplified signal reporter in a lateral flow immunoassay (LFIA), establishing a sensitive and rapid quantitative detection platform for P. aeruginosa . This AIENDs-LFIA achieved a linear detection range of 1.0 × 10 3 to 5.0 × 10 4 CFU/mL, with a detection limit of 9.74× 10 2 CFU/mL within 15 min. Comparative analysis revealed that the AIENDs-LFIA exhibits 2.2- to 5.7-fold higher sensitivity than conventional LFIAs that use fluorescent microspheres or gold nanoparticles. Furthermore, spiked-recovery experiments in tap water, mineral water, and purified water yielded acceptable recoveries and satisfactory precision. These results demonstrate the AIENDs-LFIA as a promising, efficient, and practical tool for monitoring pathogenic bacteria in drinking water.
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TOXICON
·
2025
·
IF 2.4
Ranran Ma,Fred Mwabulili,Weiwei Peng,Weibin Ma,Yuhui Yang,Yanli Xie
Fungal mycotoxins pose a serious threat to human and animal health, especially due to their co-occurrence in food and feed, causing synergistic toxicity. Current enzymatic detoxification strategies often depend on redox mediators, limiting enzyme diversity and restricting practical application. In this study, we characterized PsCopA, a novel multicopper oxidase from Pseudomonas sp., heterologously expressed in Escherichia coli , which efficiently degraded aflatoxin B 1 and zearalenone simultaneously without mediators. PsCopA showed optimal activity at 50°C (pH 7.0) for AFB 1 and 60°C (pH 8.0) for ZEN and retained over 80% activity after 2 h incubation across 20-80°C. UPLC-MS/MS analysis identified AFQ 1 and β-zearalanol as degradation products, both exhibiting significantly reduced hepatotoxicity and oxidative stress in zebrafish, even under co-exposure, suggesting effective mitigation of synergistic toxicity. Notably, PsCopA removed over 90% of toxins from contaminated cornmeal and peanut meal. These findings demonstrate PsCopA has a promising prospect for industrial application in food and feed detoxification.
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Bioresource Technology Reports
·
2026
·
IF 5
Xuwang Zhang,Huimin Li,Dongli Bao,Minghuo Wu,Hao Zhou,Yuanyuan Qu,Jingjing Zhan
Diesel contamination poses a significant threat to soil and public health. Microbial remediation, recognized for its eco-friendly benefits, necessitates the isolation of efficient diesel-degrading strains, particularly for saline-alkaline conditions. In this study, an alkali-salt-tolerant strain, Pseudomonas aeruginosa BDL, was isolated and demonstrated robust capabilities for degradation of diesel, crude oil, and petroleum hydrocarbons. Genomic analysis identified multiple hydrocarbon-degrading genes, including alkB , adhP , and ALDH , enabling its effective biodegradation of diesel pollutants. Strain BDL demonstrated moderate tolerance to saline-alkaline conditions, achieving a 72.02% removal of total petroleum hydrocarbons at pH 9, 20 g/L NaCl, and 0.5% (v/v) diesel. Bioaugmentation experiments revealed that P. aeruginosa BDL significantly enhanced diesel removal in soil. High-throughput sequencing indicated that diesel pollution reduced microbial diversity and richness. The abundance of Proteobacteria increased significantly under diesel stress, with Alcanivorax emerging as the predominant genus in both the bioaugmentation and control groups. Additionally, Pseudomonas successfully colonized the soil, contributing 5.69%–6.81% to the bioaugmented community. PICRUSt analysis suggested bioaugmentation enriched alkane-degrading genes, likely enhancing diesel biodegradation efficiency. This study highlights P. aeruginosa BDL as a promising microbial resource for bioremediation of petroleum-contaminated soils and offers practical insights for its application in environmental restoration.
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MOLECULAR PLANT PATHOLOGY
·
2026
·Vol. 27
·期 5
·e70265
·
IF 5
Yarui Yang,Jikai Yu,Jinxi Yang,Yulin Wang,Wenrui Hou,Dongfang Zhang,Jianquan Wang,Wenfeng Nie,Yan Zhang,Xiaoyun Wang,Xiuming Li,Qinghua Shi
Bacterial speck of tomato, caused by Pseudomonas syringae pv. tomato (Pst), is a globally significant disease leading to substantial yield losses. This study demonstrated that methyl caffeate (MC), a plant-derived phenolic acid, exhibited dual functionality against Pst strain DC3000 through direct antibacterial action and indirect host resistance priming. In vitro assays revealed MC's potent inhibitory activity against bacterial growth, characterised by disruption of membrane integrity, leading to intracellular content leakage and reactive oxygen species (ROS) accumulation. Transcriptomic analysis identified 434 upregulated and 326 downregulated genes, with differentially expressed genes primarily enriched in pathways related to flagellar assembly, motility, quorum sensing, chemotaxis and the type III secretion system (T3SS), thereby impairing bacterial motility and virulence. Concurrently, MC enhances plant defence responses by inducing the transcriptional expression of defence hormone signalling pathway-related genes and increasing the activities of defence enzymes such as phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO). This modulation helps maintain ROS homeostasis and alleviates oxidative damage caused by Pst infection, thereby attenuating disease progression. These findings established MC as a multifunctional agent combining direct bactericidal effects with plant immunity induction, providing a sustainable strategy for bacterial speck management.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Jingxin Ye,Weiqiang Qiu,Weiqing Lan,Jing Xie
Citral exerted its antibacterial activities in a dose-dependent manner in the three groups. • Citral increased intracellular oxidative stress levels of S. putrefaciens and P. fluorescens in mono- and co-culture. • Citral reduced motility and weakened biofilm formation of S. putrefaciens and P. fluorescens in mono- and co-culture. • Citral treatment can prolong the shelf life of large yellow croaker. Shewanella putrefaciens and Pseudomonas fluorescens , as significant specific spoilage organisms in aquatic products, can regulate cell density and a variety of physiological processes through quorum sensing (QS). This research examined the antibacterial mechanism and antivirulence efficacies of citral (CT), as a quorum sensing inhibitor (QSI), against S. putrefaciens and P. fluorescens in mono- and co-culture. Furthermore, the application of CT to large yellow croaker preservation was investigated. Results revealed that CT exerted its antibacterial activities in a dose-dependent manner. CT increased intracellular oxidative stress levels and malondialdehyde (MDA) content, decreased ATP content and peroxidase activity, and promoted the damage of cell membrane, especially in S. putrefaciens group. These two bacteria had a synergistic effect in causing spoilage through biofilm formation and motility. The antivirulence efficacies of CT against the co-culture group were weaker than those of in the mono-culture groups. CT was also found to suppress the expression of key virulence regulators in S. putrefaciens and P. fluorescens virulence, including Autoinducers-2 (AI-2) production in the QS system. Furthermore, CT had the potential capability in the preservation of large yellow croaker. Consequently, these results proved CT could combat bacteria and had antivirulence efficacies against S. putrefaciens and P. fluorescens in mono- and co-culture. Download: Download high-res image (183KB) Download: Download full-size image
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PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
·
2026
·
IF 7.9
Xin Qi,Ziwei Wang,Qixin Xia,Xiuping Liang,Guiyin Wang,Changlian Xu,Guochun Lv,Junzhuo Cai,Zhang Cheng,Zhanbiao Yang,Liling Wang,Xiaoxun Xu,Hua Yin
Although microbial degradation has been extensively studied for removing 2,2′,4,4′-tetrabrominated diphenyl ether (BDE-47), knowledge regarding the comparative efficiency and safety of degradation by facultative anaerobes under varying oxygen conditions remain limited. This study investigated the degradation of BDE-47 by Pseudomonas sp. strain QS-1 under both aerobic and anaerobic conditions. Our findings revealed that 47.5% and 12.4% of the initially added BDE-47 (0.5 mg/L) were transformed within 7 days under aerobic and anaerobic conditions, respectively. The aerobic transformation involved a complex pathway consisting of debromination, hydroxylation, ether bond cleavage, and ring opening, whereas the anaerobic transformation was limited to debromination. Toxicity assessments using an Escherichia coli model revealed that aerobic degradation products reduced cytotoxicity, whereas anaerobic degradation products induced higher rates of cell apoptosis than the parent BDE-47. Metabolomics analysis attributed this enhanced toxicity of anaerobic products to the specific inhibition of purine metabolism. In soil microcosm experiments, strain QS-1 exhibited stronger colonization and degradation performance under aerobic conditions, shifting the soil microbial community assembly towards more deterministic processes and strengthening network interactions with indigenous taxa. These findings provide insights into the biological effects of BDE-47 degradation products, and inform the assessment of efficacy and safety for bioremediation strategy. Download: Download high-res image (175KB) Download: Download full-size image
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危险品化学品经营许可证（带存储）