SCIENCE OF THE TOTAL ENVIRONMENT
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2022
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IF 10.753
Qilu Cheng,Linna Du,Ligen Xu,Yuhua Zhao,Junwei Ma,Hui Lin
Nonylphenol (NP) toxicity limits the improvements in its algal remediation efficiency. This study comprehensively investigated the performance and mechanism of NaHCO 3 -driving effects on NP-exposed algae. The results showed that NaHCO 3 enhanced algal resistance to NP and the corresponding EC 50 values increased 1.31–4.25 times. Further, the toxicological effects of NP reduced with increasing pyrenoid volume and chlorophyll and carotenoids production, and decreasing cellular damage degree. Moreover, the concentration of extracellular polymeric substances was enhanced and more NP adsorption sites were formed. Consistently, RNA-seq demonstrated significant expression alterations in genes related to energy metabolism, cellular synthesis, photosynthesis, and carbon fixation. Besides, NP biodegradation rate was increased by 15.2 % and 11.1 % in the 1, and 4 mg/L NP treatments, respectively. Identification of degradation intermediates and their toxicity via Ecological Structure Activity Relationship program showed that NaHCO 3 accelerated sequential α-C removal from NP in algae with faster generation of less toxic metabolites, namely, 4-ethylphenol, 4-cresol and 4-hydroxybenzoic acid. This study provides new insights into the role of NaHCO 3 in toxicity alleviation and metabolism enhancement of NP in algae and can assist NP bioremediation efforts in aquatic environment.
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ENVIRONMENTAL RESEARCH
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2022
·
IF 8.431
Lingyu Wen,Yiqun Huang,Weiwei Wang,Lige Zhang,Jijun Xu,Zhao Li,Ping Xu,Hongzhi Tang
Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene , are a type of organic pollutants that exist widely in the environment. Of the currently known degradation methods, bioremediation is a desirable and feasible option. A novel Diaphorobacter sp. Strain MNS-0 was isolated from saponification wastewater and showed the ability to degrade phenanthrene, fluorene, acenaphthene, anthracene , benzo[ a ]anthracene, or chrysene using phenanthrene as the sole carbon source. Gas chromatography mass spectroscopy analysis of catabolic intermediates indicates that phenanthrene degradation occurs through the phthalic acid pathway in strain MNS-0. Genome sequencing shows that strain MNS-0 has two plasmids and one chromosome containing a presumptive phenanthrene degradation gene cluster. Strain MNS-0 was able to completely degrade 100 mg/L phenanthrene within 40 h and tolerate up to 10 g/L NaCl at pH 9.0, while maintaining phenanthrene degradation activity. We thus propose that strain MNS-0 is an effective degrader for bioremediation of PAHs pollution, even in relatively harsh alkali environments such as saponification wastewater.
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Frontiers in Microbiology
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2022
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IF 6.064
Li Bing,Wu Di,Li Yan,Shi Yan,Wang Chenlin,Sun Jiasi,Song Chunfeng
Antibiotic resistance is one of the most important environmental challenges. Microalgae has been considered as a promising green media for environmental purification. In this work, sulfadimethoxine (SDM) biodegradation potential of Chlorella sp. L38 and Phaeodactylum tricornutum MASCC-0025 is investigated. Experimental results indicated that the tested freshwater and marine microalgae strains presented stress response to SDM addition. For Chlorella sp. L38, it has a good adaptability to SDM condition via antioxidant enzyme secretion (SOD, MDA, and CAT up to 23.27 U/mg, 21.99 μmol/g, and 0.31 nmol/min/mg) with removal rate around 88%. P. tricornutum MASCC-0025 exhibited 100% removal of 0.5 mg/L SDM. With increasing salinity (adding a certain amount of NaCl) of cultivation media, the removal rate of SDM by microalgae increased. Although its adaptive process was slower than Chlorella sp. L38, the salinity advantage would facilitate enzyme accumulation. It indicated that microalgae could be used to remove SDM from freshwater and marine environment via suitable microalgae strain screening.
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PROTEIN EXPRESSION AND PURIFICATION
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2022
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IF 2.025
Xiaowen Wang,Sibo Chen,Lin Fan,Hao Liu,Si Chen,Xianfeng Ye,Zhoukun Li,Zhongli Cui,Yan Huang
A new glycoside hydrolase family 2 (GH2) β-galactosidase encoding gene galM was cloned from Microvirga sp. strain MC18 and overexpressed in Escherichia coli . The recombinant β-galactosidase GalM showed optimal activity at pH 7.0 and 50 °C, with a stability at pH 6.0–9.0 and 20–40 °C, which are conditions suitable for the diary environment. The K m and V max values for o-nitrophenyl-β- d -galactopyranoside (oNPG) were 1.30 mmol/L and 15.974 μmol/(min·mg), respectively. GalM showed low product inhibition by galactose with a K i of 73.18 mM and high tolerance for glucose that 86.5% activity retained in the presence of 500 mM glucose. It was also stable and active in 20% of methanol, ethanol and isopropanol. In addition, the enzyme activity of GalM was activated significantly over 0–2 mol/L NaCl (1.6–4.3 fold). These favorable properties make GalM a potential candidate for the industrial application.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2023
·Vol. 107
·期 11
·3745-3761
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IF 5
Xu Chenglong,Lin Wenxi,Chen Yunni,Gao Boliang,Zhang Zhibin,Zhu Du
Perylenequinones (PQs) are a class of natural polyketides used as photodynamic therapeutics. Heat stress (HS) is an important environmental factor affecting secondary metabolism of fungi. This study investigated the effects of HS treatment on PQs biosynthesis of Shiraia sp. Slf14(w) and the underlying molecular mechanism. After the optimization of HS treatment conditions, the total PQs amount reached 577 ± 34.56 mg/L, which was 20.89-fold improvement over the control. Also, HS treatment stimulated the formation of intracellular nitric oxide (NO). Genome-wide analysis of Shiraia sp. Slf14(w) revealed iNOSL and cNOSL encoding inducible and constitutive NOS-like proteins (iNOSL and cNOSL), respectively. Cloned iNOSL in Escherichia coli BL21 showed higher nitric oxide synthase (NOS) activity than cNOSL, and the expression level of iNOSL under HS treatment was observably higher than that of cNOSL , suggesting that iNOSL is more responsible for NO production in the HS-treated strain Slf14(w) and may play an important role in regulating PQs biosynthesis. Moreover, the putative biosynthetic gene clusters for PQs and genes encoding iNOSL and nitrate reductase (NR) in the HS-treated strain Slf14(w) were obviously upregulated. PQs biosynthesis and efflux stimulated by HS treatment were significantly inhibited upon the addition of NO scavenger, NOS inhibitor, and NR inhibitor, indicating that HS-induced NO, as a signaling molecule, triggered promoted PQs biosynthesis and efflux. Our results provide an effective strategy for PQs production and contribute to the understanding of heat shock signal transduction studies of other fungi.
Key points
• PQs titer of Shiraia sp. Slf14(w) was significantly enhanced by HS treatment . • HS-induced NO was first reported to participate in PQs biosynthetic regulation . • Novel inducible and constitutive NOS-like proteins (iNOSL and cNOSL) were obtained and their NOS activities were determined .
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2021
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IF 3.575
Zhimao Mai,Lin Wang,Qiqi Li,Yingting Sun,Si Zhang
The bacterium Gordonia sp. SCSIO19801, which could effectively utilize phenanthrene as the sole carbon source, was isolated from the seawater of the South China Sea. Its biodegradation characteristics, whole genome sequence, and biodegradation pathway were investigated. The phenanthrene biodegradation process of Gordonia sp. SCSIO19801 was estimated to be a first-order kinetic model with a k value of 0.26/day. Based on the identification of metabolites, utilization of probable intermediates, and genomics analysis of related genes, the degradation of phenanthrene by Gordonia sp. SCSIO19801 was proposed to occur via the salicylate metabolic pathway. This is the first report of a phenanthrene degradation pathway in Gordonia species. In addition, the Gordonia sp. SCSIO19801 could use other aromatic compounds as the sole source of carbon and energy. These characteristics indicate that Gordonia sp. SCSIO19801 can be utilized for developing effective methods for the biodegradation of petroleum hydrocarbons in marine environments.
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PETROLEUM SCIENCE AND TECHNOLOGY
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2019
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IF 1.07
Lin Li,Xuwei Liu,Huijing Wang,Chaocheng Zhao,Qiyou Liu,Xiufang Shang
Environmental adaptability and biodegradability of oil pollutants on functional bacteria are two important keys to bioremediation technologies. Pseudomonas sp. LKY-5 revealed strong biological activities in high salinity (3%) and high alkalinity (pH 9.0). N-hexadecane, phenanthrene, pyrene and carbazole could be directly utilized, and inhibited by the presence of DBT. Sulfur compounds and alkanes in No. 0 diesel and FCC diesel were effectively degraded. The concentrations of short chain alkanes were increased significantly after degradation. It can be seen that strain LKY-5 is an excellent bacterial candidate for bioremediation of oil-contaminated sites and sediments.
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Innovative Food Science & Emerging Technologies
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2018
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IF 3.116
Debao Niu,Lang-Hong Wang,Xin-An Zeng,Qing-Hui Wen,Charles S. Brennan,Zhong-Sheng Tang,Man-Sheng Wang
The effects of low concentrations of ethanol (3% to 9%, v/v) on cell membrane fatty acid composition , membrane lipid conformation, membrane fluidity and pulsed electric fields (PEF) resistance of Acetobacter sp. in the stationary phase were investigated. Results showed that the growth of Acetobacter sp. was inhibited, and analysis of gas chromatography–mass spectrometry, Raman spectroscopy and fluorescence polarization indicated that membrane fluidity of Acetobacter sp. cells was significantly increased by modifying membrane fatty acid composition and membrane lipid conformation after exposure to ethanol. Moreover, results showed that cells cultivated in the medium with relatively high ethanol concentrations were more vulnerable to PEF. For example, the inactivation of Acetobacter sp. (10 9 CFU/mL) cultivated with 9% ethanol by PEF (20.0 kV/cm, 6.0 ms) could reach 5.17 log, significantly higher than those cultivated without ethanol (3.22 log). This was confirmed by morphological analysis, showing various changes on the cell surface after PEF treatment. Industrial relevance Acetobacter sp. is one of spoilage microorganisms in winemaking, and it is able to convert the ethanol produced by yeasts into acetic acid and thereby increase volatile acidity in wine. In this study, the effect of ethanol as a growth substrate on PEF resistance in Acetobacter sp. cells was investigated. Results here reported will contribute to better understand the influencing mechanisms of ethanol on the inactivation of Acetobacter sp. cells by PEF treatment and further develop the application of PEF for inactivating acetic acid bacteria (AAB) in the wine industry.
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Marine Drugs
·
2022
·Vol. 20
·期 8
·533
·
IF 6.085
Juanjuan Wang,Zebin Liu,Xiaowei Pan,Ning Wang,Legong Li,Yuguang Du,Jianjun Li,Mei Li
Fucoidans represent a type of polyanionic fucose-containing sulfated polysaccharides (FCSPs) that are cleaved by fucoidan-degrading enzymes, producing low-molecular-weight fucoidans with multiple biological activities suitable for pharmacological use. Most of the reported fucoidan-degrading enzymes are glycoside hydrolases, which have been well studied for their structures and catalytic mechanisms. Little is known, however, about the rarer fucoidan lyases, primarily due to the lack of structural information. FdlA fromFlavobacteriumsp. SA-0082 is an endo-type fucoidan-degrading enzyme that cleaves the sulfated fuco-glucuronomannan (SFGM) through a lytic mechanism. Here, we report nine crystal structures of the catalytic N-terminal domain of FdlA (FdlA-NTD), in both its wild type (WT) and mutant forms, at resolutions ranging from 1.30 to 2.25 Å. We show that the FdlA-NTD adopts a right-handed parallel β-helix fold, and possesses a substrate binding site composed of a long groove and a unique alkaline pocket. Our structural, biochemical, and enzymological analyses strongly suggest that FdlA-NTD utilizes catalytic residues different from other β-helix polysaccharide lyases, potentially representing a novel polysaccharide lyase family.Keywords:fucoidan lyase;polysaccharides;crystal structure;catalytic mechanism
研究领域:
生物化学
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结构生物学
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Frontiers in Bioengineering and Biotechnology
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2022
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IF 6.064
Ma Anzhou,Ma Jianpeng,Chen Xianke,Zhuang Guoqiang
Highly chlorinated benzenes usually have a low efficient degradation in environment. Here we proposed a synergistic removal strategy of pentachlorobenzene (PeCB) using Pseudomonas sp. JS100 coupled with immobilized nanoscale zero-valent iron (NZVI). The structural and textural features of the synergistic system were characterized by X-ray powder diffraction, field emission scanning electron microscopy, and a specific surface area and pore size analysis. Nanoscale zero-valent iron particles were dispersed and attached to the biofilter, which increased the specific surface area to 34.5 m2 g−1. The batch experiment revealed that the removal efficiency of PeCB reached 80.2% in the synergistic system within 48 h. The degradation followed pseudo-first-order reaction kinetics, and the reaction rate constant was measured to be 0.0336 h−1. In the degradation mechanism, PeCB was degraded by NZVI to lower chlorobenzenes, which were utilized by Pseudomonas sp. JS100 as nutrients, thereby achieving rapid removal of PeCB.
研究领域:
环境科学
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微生物学
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
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IF 4.192
Miaomiao Li,Jinlong Song,Qingyun Ma,Delong Kong,Yiqing Zhou,Xu Jiang,Rebecca Parales,Zhiyong Ruan,Qinghua Zhang
A total of five strains of nicosulfuron-degrading bacteria were isolated from a continuously cultivated microbial consortium using culturomics. Among them, a novel Pseudomonas strain, LAM1902, with the highest degradation efficiency was investigated in detail. The characteristics of nicosulfuron-degradation by LAM1902 were investigated and optimized by response surface analysis. Furthermore, non-targeted metabolomic analysis of extracellular and intracellular biodegradation of nicosulfuron by LAM1902 was carried out by liquid chromatography/mass spectroscopy (LC–MS) and gas chromatography–time-of-flight/mass spectroscopy (GC–TOF/MS). It was found that nicosulfuron was degraded by LAM1902 mainly via breaking the sulfonylurea bridge, and this degradation might be attributed to oxalate accumulation. The results of GC–TOF/MS also showed that the intracellular degradation of nicosulfuron did not occur. However, nicosulfuron exerted a significant influence on the metabolism of inositol phosphate, pyrimidine, arginine/proline, glyoxylate, and dicarboxylate metabolism and streptomycin biosynthesis. The changes of myo-inositol, trehalose, and 3-aminoisobutanoic acid were proposed as a mechanism of self-protection against nicosulfuron stress.
研究领域:
微生物学
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环境科学
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植物保护
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2013
·Vol. 97
·期 23
·9941-9954
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IF 3.689
Zhang Jinhua,Shi Junling,Liu Yanlin
To identify the substrates and enzymes related to resveratrol biosynthesis in Alternaria sp. MG1, different substrates were used to produce resveratrol, and their influence on resveratrol production was analyzed using high performance liquid chromatography (HPLC). Formation of resveratrol and related intermediates was identified using mass spectrum. During the biotransformation, activities of related enzymes, including phenylalanine ammonia-lyase (PAL), trans -cinnamate 4-hydroxylase (C4H), and 4-coumarate-CoA ligase (4CL), were analyzed and tracked. The reaction system contained 100 mL 0.2 mol/L phosphate buffer (pH 6.5), 120 g/L Alternaria sp. MG1 cells, 0.1 g/L MgSO 4 , and 0.2 g/L CaSO 4 and different substrates according to the experimental design. The biotransformation was carried out for 21 h at 28 °C and 120 rpm. Resveratrol formation was identified when phenylalanine, tyrosine, cinnamic acid, and p -coumaric acid were separately used as the only substrate. Accumulation of cinnamic acid, p -coumaric acid, and resveratrol and the activities of PAL, C4H, and 4CL were identified and changed in different trends during transformation with phenylalanine as the only substrate. The addition of carbohydrates and the increase of phenylalanine concentration promoted resveratrol production and yielded the highest value (4.57 μg/L) when 2 g/L glucose, 1 g/L cyclodextrin, and phenylalanine (4.7 mmol/L) were used simultaneously.
研究领域:
微生物学
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生物化学
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