FOOD CHEMISTRY
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2025
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IF 9.8
Yuanxiu Liu,Tong Shi,Xiyue Cao,Xin Wang,Mengzhe Li,Chenya Lu,Xiaoli Zhang,Ying Tian,Li Yuan,Wengang Jin,Ruichang Gao
This study explored the influences of L-Arg in the ion migration and the quality of low-salt fish balls (L, 0.3 % NaCl). L-Arg significantly increased the weight gain and expansion rate of L by 18.33 % and 13.47 %. Water-holding capacity decreased from the internal to the external part of fish balls, consistent with the looser microstructure. Inductively coupled plasma-mass spectrometry (ICP-MS) revealed that L-Arg promoted Na + migration at setting ( P < 0.05), decreased the internal concentration from 34.38 to 30.37 mg/L and increased the external one from 25.78 to 29.80 mg/L ( P < 0.05). Conversely, Cl − concentration was decreased at setting and improved after the two-stage heating ( P < 0.05), indicating the suppressed Cl − migration. The partial replacement of Na + by L-Arg facilitated the protein unfolding, formation of disulfide bonds and exposure of tryptophan residues. This study enriched the mechanisms of salt reduction and quality maintenance for surimi products regarding ion migration.
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EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
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2026
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IF 5
Xiaoyang Li,Xiaodan Wang,Yan Ming,Rongtao Li,Yun Yang,Jing Wang,Baiyu Chang,Qing Zhang,Jingling Tang,Jiaxin Liu,Hongyu Ji,Congcong Lin
Multifunctional microneedles MR-NPs-MNs was developed for chronic wound treatment, loading mupirocin and L-arginine into PLGA nanoparticles. • The MR-NPs-MNs penetrate the stratum corneum, facilitating deep drug delivery and a synergistic antibacterial effect with mupirocin. • Co-encapsulation of mupirocin and L-arginine in MR-NPs synergizes immediate antibacterial action with wound microenvironment remodeling. • MR-NP s-MNs simultaneously address infection, inflammation and tissue repair to accelerate wound healing. Chronic wounds pose notable clinical challenges due to persistent infection, inflammation, and impaired tissue regeneration. We designed a multi-functional microneedle patch (MR-NPs-MNs) comprising chitosan-based microneedles encapsulating PLGA nanoparticles co-loaded with mupirocin and L-arginine. As a key component, L-arginine significantly enhances anti-inflammatory effects by reducing pro-inflammatory cytokines and effectively promotes tissue repair by regulating oxidative stress and facilitating angiogenesis. The PLGA nanoparticles MR-NPs exhibited stable physical properties (181.7 ± 5.875 nm particle size, 22.4 ± 0.529 mV zeta potential) and were incorporated into microneedles capable of withstanding 40.38 N compression. Notably, MR-NPs-MNs outperformed conventional mupirocin ointment, achieving a 99.82 ± 0.15% antibacterial rate (vs. 90.69 ± 1.11% for the ointment), superior biofilm inhibition (96.82 ± 0.83% and 77.8 ± 5.32%), and enhanced transdermal drug delivery. In vitro, MR-NPs-MNs increased NO production, reduced TNF-α, IL-6 and ROS levels, and promoted wound healing. In diabetic wound models, MR-NPs-MNs increased the healing rate by 1.54-fold, significantly reduced bacterial counts ( P < 0.01) by day 9, and improved key healing parameters including collagen matrix reorganization, robust neovascularization, and functional tissue formation. With its biocompatible components, this system provides a potential local therapeutic platform for infected diabetic wound treatment. Download: Download high-res image (153KB) Download: Download full-size image
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TALANTA
·
2026
·
IF 6.7
Xuedong Jia,Hongyuan Guo,Xiu Zhao,Zhuo Shi,Yingchun Zhu,Suozhu Wu
Monitoring nitrite in water and food is critical for ensuring human health. Herein, an electrochemical sensor was constructed for determining nitrite based on iodide probe and gold nanoparticles-poly(L-arginine)-poly(L-lysine) (AuNPs-PArg-PLys) modified electrode. The AuNPs-PArg-PLys nanocomposites were fabricated by one-step in-situ electrochemical synthesis method. Electrochemical detection of nitrite was carried out using square-wave voltammetry. With the assistance of iodide probe, the minimum detectable nitrite concentration reduced remarkably by five orders of magnitude when compared to that without this probe. The iodide-assisted AuNPs-PArg-PLys-based electrochemical sensor could determine nitrite from 1.0 nM to 10.0 μM with a detection limit of 271.8 pM. This sensor possessed the advantages of simplicity, rapidness, low detection limit, wide response range, excellent reproducibility, and high selectivity towards nitrite, which was successfully utilized to measure nanomolar-scale nitrite in tap water and beef. The proposed sensor offered potential applications in environmental protection and food safety.
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Food Chemistry-X
·
2026
·
IF 9.2
Rong Ma,Chen Chen,Zihang Shi,Yangyang Hu,Yangying Sun,Qiang Xia,Changyu Zhou,Daodong Pan
Although pulsed electric field (PEF) and L-arginine alone can improve meat tenderness, their synergistic molecular mechanism remains unclear. This study first explored the tenderization mechanism of combined PEF and L-arginine (LP) in goose meat. Results showed that LP reduced goose meat shear force by 51.30%, with effects significantly superior to either treatment alone. Hematoxylin-eosin staining revealed that LP enlarged intermuscular spaces. Infrared spectroscopy indicated that LP induced a conformational shift from α-helix to β-sheet in myofibrillar proteins (MPs). Fluorescence spectroscopy showed a significantly increased fluorescence intensity, confirming structural unfolding of MPs. Enzyme activity assays showed that LP elevated calpain activity by 64.33%. SDS-PAGE further confirmed the accelerated degradation of troponin-T. Molecular dynamics simulations revealed that PEF primarily drives the restructuring of the enzyme-substrate binding interface, while L-arginine stabilizes the process by maintaining the hydrogen bond network. This study provides a theoretical basis for developing efficient and controllable meat tenderization technologies.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2020
·
IF 3.573
Ruizhang Xu,Lei Ding,Dandan Chen,Tianyu Liu,Yunqi Wu,Ya Cao,Jinyao Chen,Feng Yang,Jian Kang,Ming Xiang
Polyamide thin-film composite reverse osmosis (RO) membranes face poor chlorine resistance and antifouling properties, which greatly limit the wide application of RO technology. To evidently enhance chlorine stability, antifouling properties, and the permeability of the RO membrane, in this research, L-arginine (Arg) had been grafted on polyvinyl alcohol (PVA) first, and the functionalized PVA (denoted as PVA–Arg) had been grafted on the RO membrane surface subsequently. Results of X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy indicated the successful synthesis of PVA–Arg as well as the successful grafting of PVA or PVA–Arg on the RO membrane surface. The permeability, chlorine resistance property, and antifouling properties of all RO membranes were tested. The PVA- or PVA–Arg-grafted RO membranes showed obvious enhancements in permeability, chlorine resistance property, and antifouling properties. For PVA-modified RO membranes, the bonus PVA layer deteriorated its water flux while filtrating. However surprisingly, the PVA–Arg-grafted RO membranes exhibited excellent enhancement in water flux and antifouling properties. For the optimized sample, the pure water flux of the PVA–Arg-grafted RO membrane is 8.3% higher than the nascent RO membrane (57.2 L/m2 h compared with 52.8 L/m2 h); the salt rejection of membranes is promoted to around 99.50% from the original 95.58%. Meanwhile, the antifouling properties of PVA–Arg-grafted RO membranes are further enhanced than that of PVA-grafted RO membranes.
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JOURNAL OF BIOMATERIALS APPLICATIONS
·
2019
·
IF 2.442
Xiaoling Yang,Xue Liu,Qianqian Wu,Xu Lv,Bo Li,Hongzhe Sun,Wenfeng Xu,Xiaoling Liao
Due to their excellent biocompatibility and suitable elastic modulus, carbon–carbon composites have excellent prospects for a wide range of applications such as artificial joints and bone replacements. However, the hydrophobicity and poor bioactivity limit their application in bone repair and substitution. Herein, a two-step surface modification of carbon–carbon composites was proposed to overcome these disadvantages. A polydopamine (PDA) layer was first deposited on the surface of carbon–carbon composites, followed by covalent coupling with arginine–glycine–aspartic acid (RGD) in varying concentrations. The surface-modified materials were characterized using a variety of techniques including water contact angle, scanning electron microscopy, atomic force microscopy, and protein adsorption. In addition, the cellular proliferation, alkaline phosphatase activity, and real-time quantitative PCR (RT-PCR) analysis were further tested in vitro. The results indicated that polydopamine and RGD nanoparticles formed on the surface, which significantly enhanced the hydrophilicity following modification. The RGD–PDA-coated carbon–carbon composites exhibited excellent roughness, protein adsorption behavior, and uniform morphology when a concentration of 50 µg/mL RGD was used, unlike blank and polydopamine-modified carbon–carbon composites. The in vitro cell co-culture experiments demonstrated that proliferation and alkaline phosphatase expression in osteoblasts was greatly improved after RGD–PDA coating. The RT-PCR results revealed that the expression of differentiation markers (alkaline phosphatase and osteocalcin) in cells on the RGD–PDA-coated carbon–carbon composites was higher than that on the blank and polydopamine-modified carbon–carbon composites, indicating that the RGD–PDA coating could support cell proliferation and promote osteogenic differentiation of MC3T3-E1 cells. In particular, the cells cultured on this material grew linearly along the morphological features of the material, which was effective for the subsequent regulation of cell growth. Our study suggested that the RGD–PDA coating was an effective method of enhancing the hydrophobicity and the cellular bioactivity of carbon–carbon composites, thus extending applications of carbon–carbon composites to the bone repair field.
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CURRENT PHARMACEUTICAL DESIGN
·
2023
·
IF 3.1
Yi Shi,Qian Wu,Yi Lu,Ling-Peng Meng,Xiao-Ling Xu,Xiao-Juan Wang,Wei Chen
Aim We aimed to design RGD-anchored liposomes encapsulating an antipyroptosis drug that could efficiently target macrophages and relieve the rate of cytokine release syndrome, providing a new strategy for sepsis treatment, especially sepsis-induced acute renal injury. Background Sepsis is a clinical syndrome of life-threatening organ dysfunction caused by host response disorders due to infection. Sepsis has a high incidence and remains one of the leading causes of death worldwide. Objective Macrophage-mediated pyroptosis plays an important role in the occurrence and development of cytokine release syndrome and organ injury caused by sepsis. Curcumin can inhibit inflammasome assembly and slow the progression of pyroptosis by scavenging intracellular reactive oxygen species, but it has poor water solubility and low bioavailability. The emergence of drug-delivery nanosystems has overcome this problem, but there is still a lack of research on how to accurately deliver antipyroptotic drugs to innate immune cells and subsequently hinder pyroptosis. Methods We constructed a curcumin-loaded RGD-modified liposome (RGD-lipo/Cur) and demonstrated that RGD-lipo/Cur could effectively target macrophages. Results In vitro , RGD-lipo/Cur reduced the upregulation of caspase-1, caspase-3, NLRP3, IL-1β and GSDMD, inhibiting pyroptosis, reducing oxidative stress, and attenuating the proinflammatory cytokine cascade. Conclusion RGD-lipo/Cur was considered to have great potential for sepsis treatment.
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ACS Applied Bio Materials
·
2019
·
IF 0
Jingyuan Ma,Peizhi Yu,Boru Xia,Yuxiu An,Zhiqiao Wang
We synthesized a biodegradable and environmentally friendly shale inhibitor based on chitosan-grafted l-arginine (CA) for wellbore stability in shale formation. The structure of CA was characterized by Fourier-transform infrared spectroscopy. Linear swelling, shale hot-rolling recovery, shale inhibition durability, and sedimentation experiments were used to evaluate the inhibition properties of CA and compared with the commonly used inhibitors potassium chloride (KCl) and polyamines (HPA and SIAT). The results showed that the inhibition of CA was better than that of KCl, HPA, and SIAT and that it can have a shale hot-rolling recovery of more than 90% at 150 °C, which indicated that CA had higher temperature resistance and longer durability. More importantly, it can be biodegraded as exhibited by the biodegradibility experiment. The inhibition mechanism of CA was studied by particle size distribution, X-ray diffraction, scanning electron microscopy, zeta potential analysis, and contact angle test. The strong inhibition of CA can be attributed to its encapsulation of MMT and shale surfaces. The CA with strongly positively charge was firmly adsorbed on the surface of MMT and shale, which not only neutralized the negative charge of MMT, compressed the diffused electric double layer, but also increased the contact angle of MMT and shale surface which enhancing hydrophobicity of MMT and shale. The hydration swelling and dispersion of MMT and shale were further inhibited. In addition, compatibility experiments showed that CA was compatible with commonly used treatment agents. CA did not affect the rheology of water-based drilling fluids and can reduce fluid loss after aging.
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MACROMOLECULAR BIOSCIENCE
·
2015
·Vol. 15
·期 6
·829-838
·
IF 3.851
Haiyang Yu,Zhaohui Tang,Dawei Zhang,Wantong Song,Taicheng Duan,Jingkai Gu,Xuesi Chen
A novel random copolypeptide of ornithine, arginine, glycine, and aspartic acid [Poly(ornithine-co-arginine-co-glycine-co-aspartic acid), Poly(O,R,G,D)] has been prepared through ring-opening polymerization of N -δ-carbobenzoxy-l-ornithine N -carboxyanhydride [Orn(Cbz)-NCA)], l-glycine N -carboxyanhydride (Gly-NCA) and β-benzyl l-aspartate N -carboxyanhydride [Asp(Bn)-NCA], following by subsequent deprotection and guanidization. The structure of Poly(O,R,G,D) was confirmed by nuclear magnetic resonance (NMR) spectroscopy and gel permeation chromatography (GPC). Low cytotoxicity of Poly(O,R,G,D) was confirmed from MTT assay. The Poly(O,R,G,D) contain some internal sequences of RXXR ( X = O, R, G, or D) that could be proteolytically cleaved to expose the cryptic CendR element and bind to Neuropilin-1. This would lead to vascular and tissue permeabilization. Therefore trypsin-cleaved Poly(O,R,G,D) increase the vascular leakage of Evans blue from dermal microvessels at the injection site in vivo skin permeability assay. The intratumoral injection of the Poly(O,R,G,D) significantly enhanced the concentration of cisplatin-loaded nanoparticles in MCF-7 solid tumors. These results show that Poly(O,R,G,D) could increase the vascular leakage and tissue penetration of nanoparticles in a solid tumor and can be used as a potential polymeric tumor-penetrating agent.
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FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Li-Feng Li,Meng-Di Wang,Chen-Yang Zhang,Meng-Yao Jin,Hua-Lei Chen,Huan Luo,Tian-Yu Hou,Zhi-Jun Zhang,He Li
Advanced glycation end products (AGEs) generated from the Maillard reaction (MR) during food processing in the human physiological environment, have been proven to be significantly associated with various chronic metabolic diseases. In this study, 18 flavonoids were investigated to study their effects on AGEs formation during MR within a glucose-lysine-arginine model system. Five AGEs— N ε -carboxymethyl-lysine (CML), N ε -carboxyethyl-lysine (CEL), pyrraline, pentosidine, and argpyrimidine—were determined by high-performance liquid chromatography-mass spectrometry, with inhibitory rates ranging between 0 % and 71.35 %. Isorhamnetin and naringenin exhibited the strongest inhibitory effect on the formation of CML and CEL, respectively, whereas myricetin exhibited the strongest inhibitory effect on pyrraline, pentosidine, and argpyrimidine formation. In addition, each flavonoid was reacted with glyoxal (GO) and methylglyoxal (MGO) to investigate their trapping activities and adducts. The results showed that the 18 flavonoids could effectively clear GO and MGO, with clearance rates of 1.03 %–71.42 % and 0.93 %–69.37 % for GO and MGO, respectively. Six flavonoids—chrysin, naringenin, apigenin, luteolin, diosmetin, and kaempferol—could form adduct products with mono-/di-MGO, with flavonoid to mono-/di-MGO adduct ratios of approximately 6:1, 2:1, 8:1, 23:1, 10:1, and 3:1, respectively. The number and site of phenolic hydroxyl groups, as well as methoxy substitution on the B-ring in flavonoids, had little effect, but phenolic hydroxyl groups at the C-3 position in the C-ring impeded adduct formation. The flavonoids lowered the degradation of glucose itself, and decreased ammonia-induced degradation and Amadori rearrangement product oxidation due to their antioxidant activities, and they trapped the reactive 1,2-di‑carbonyl species via nucleophilic addition reaction to form AGEs.
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Acta Biomaterialia
·
2016
·
IF 6.008
Qianming Lin,Yumeng Yang,Qian Hu,Zhong Guo,Tao Liu,Jiake Xu,Jianping Wu,Thomas Brett Kirk,Dong Ma,Wei Xue
Hydrogels have attracted much attention in cancer therapy and tissue engineering due to their sustained gene delivery ability. To obtain an injectable and high-efficiency gene delivery hydrogel, methoxypolyethylene glycol (MPEG) was used to conjugate with the arginine-functionalized poly( l -lysine) dendron (PLLD-Arg) by click reaction, and then the synthesized MPEG-PLLD-Arg interacted with α-cyclodextrin (α-CD) to form the supramolecular hydrogel by the host-guest interaction. The gelation dynamics, hydrogel strength and shear viscosity could be modulated by α-CD content in the hydrogel. MPEG-PLLD-Arg was confirmed to bind and deliver gene effectively, and its gene transfection efficiency was significantly higher than PEI-25k under its optimized condition. After gelation, MMP-9 shRNA plasmid (pMMP-9) could be encapsulated into the hydrogel matrix in situ and be released from the hydrogels sustainedly, as the release rate was dependent on α-CD content. The released MPEG-PLLD-Arg/pMMP-9 complex still showed better transfection efficiency than PEI-25k and induced sustained tumor cell apoptosis. Also, in vivo assays indicated that this pMMP-9-loaded supramolecular hydrogel could result in the sustained tumor growth inhibition meanwhile showed good biocompatibility. As an injectable, sustained and high-efficiency gene delivery system , this supramolecular hydrogel is a promising candidate for long-term gene therapy. Statement of Significance To realize the sustained gene delivery for gene therapy, a supramolecular hydrogel with high-efficiency gene delivery ability was prepared through the host-guest interaction between α-cyclodextrin and PEGylated arginine-functionalized poly( l -lysine) dendron. The obtained hydrogel was injectable and biocompatible with adjustable physicochemical property. More importantly, the hydrogel showed the high-efficiency and sustained gene transfection to our used cells, better than PEI-25k. The supramolecular hydrogel resulted in the sustained tumor growth inhibition meanwhile keep good biocompatibility. As an injectable, sustained and high-efficiency gene delivery system, this supramolecular hydrogel is a promising candidate in long-term gene therapy and tissue engineering.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jinghe Sun,Yimeng Li,Tingting Yan,Jingfeng Yang
An edible composite film was developed and applied for ready-to-eat sea cucumber storage to improve the product quality. The PAC film base is first prepared by mixing 0.5 % glycerin (GL) with 4 % polyvinyl alcohol (PVA) and 1 % arginine-modified chitosan (Arg-CTS) in the same volume. After the addition of nano-ZnO (ZnO) and thymol (Thy) to the PAC film base, the mechanical properties and functions were tested. Compared to the PAC film, the PAC-ZnO-ThyH composite film showed a 1.34-fold increase in the DPPH scavenging rate and a 2.19-fold increase in the ABTS scavenging rate. Contrary to the PAC film, the inhibition zone diameter of Escherichia coli and Staphylococcus aureus significantly increased by 2.35 and 4.08 folds in the PAC-Zno-ThyH film, respectively. After applying the PAC-ZnO-ThyH film to store ready-to-eat sea cucumber for 10 days, there was a significant reduction in weight loss, total volatile basic nitrogen (TVB-N), and lipid oxidation levels to 1.47 and 1.26 folds to the Ctrl group. After preservation, the hardness and chewiness of ready-to-eat sea cucumber were maintained at 1079.62 ± 138.86 N and 913.73 ± 175.79 N, respectively. The novel PAC-ZnO-ThyH composite film can be used as an active food packaging for promising seafood applications.
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