FOOD CONTROL
·
2024
·
IF 5.6
Huilin Lu,Sai Li,Qunyu Gao
In this study, an active gel of κ-carrageenan (KC) loaded with carvacrol/hydroxylpropyl-β-cyclodextrin (CAR/HPβCD) composite was prepared, its antibacterial property and application in strawberry preservation were evaluated. The successful preparation of CAR/HPβCD composite was verified by Fourier transform infrared spectroscopy (FT-IR) and 1 H NMR, and the embedding mechanism was further elucidated by the molecular docking and molecular dynamics simulation. Then the CAR/HPβCD composites were incorporated into KC to fabricate the active gels. The KC-based gels with great thermal reversibility presented sol states at high temperature and formed gels upon cooling. During the cooling process, KC transformed from random coil conformations to rigid helical rods. A large three-dimensional network structure was eventually constructed through the continuous aggregation of the double helices. It showed that the concentrations of the composites affected both the microstructures and properties of the gels. Low composite concentrations were beneficial to increase the hardness, gumminess and chewiness of the gels, while high composite concentrations could remarkably improve the water retention, freeze-thaw stability and in vitro antibacterial activity of the gels. The antibacterial activity of the gel containing 20% CAR/HPβCD composite against staphylococcus aureus ( S. aureus) could last 15 days. Furthermore, the active gel used as a fresh-keeping coating for strawberry preservation could effectively delay the decline of weight, titratable acidity (TA), total soluble solids (TSS) and firmness of strawberries during the storage time, and reduce the number of rotten strawberries by three quarters compared with the control group within 7 days. The active gel reported in this study was a promising material for food preservation.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Huajun Kang,Tengfei Fan,Zihan Lin,Yanxia Shi,Xuewen Xie,Lei Li,Sheng Xiang,Xiaowei Yuan,Xingsheng Li,Baoju Li,Ali Chai
Clubroot, caused by the obligate parasite Plasmodiophora brassicae , is one of the most important diseases of brassicas. The antagonistic bacterium Paenibacillus polymyxa ZF129 can suppress clubroot while its effectiveness is often unstable. To control clubroot more effectively, the macrobeads for controlled release of ZF129 were prepared using microencapsulation technology. Macrobeads with various ratios of chitosan (2 % w/w): carrageenan (0.3 % w/v) were prepared by an ionotropic gelation method and the bacteria ZF129 was loaded into macrobeads. The 1:1 chitosan: carrageenan showed the maximum swelling ratio (634 %), and the maximum survival rate (61.52 ± 1.12 %) after freeze-drying. Fourier transform infrared revealed the electrostatic interactions between chitosan and carrageenan. The macrobeads can efficiently release ZF129 strains into phosphate buffer solution and reach equilibrium in 48 h. The maximum number of bacteria cells to be released in the soil was observed after 25–30 days. The control efficacy of ZF129 macrobeads (chitosan: carrageenan, 1:1) and ZF129 culture against clubroot disease was 76.33 ± 3.65 % and 59.76 ± 4.43 % in greenhouse experiments, respectively and the control efficacy was calculated as 60.74 ± 5.00 % for ZF129 macrobeads and 40.94 ± 4.05 % for ZF129 culture under field experiments, respectively. The ZF129 macrobeads had significant growth-promoting effects on pak choi and Chinese cabbage. The encapsulation method described in this study is a prudent approach toward efficient biopesticides utilization with reduced environmental implications.
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高影响因子
INFLAMMATION
·
2024
·
IF 4.5
Liu Qi,Zhao Yubo,Dong Shuai,Bai Xingyuan,Chen Bin,Liu Xijuan,Shen Jing,Zhu Dan
Deciphering the complex and redundant process of acute inflammation remains challenging. The failure of numerous clinical trials assessing anti-inflammation agents which had promising preclinical effects inevitably questions the validity of current animal models of inflammation. This study aimed to better understand the process of immune inflammatory response and to select more suitable models to evaluate the effect of potential anti-inflammatory drugs. Zymosan and λ-carrageenan are the most used representatives of particulate and soluble irritants that trigger acute inflammation in the air pouch inflammation model. When zymosan was used, the number of exudate cells first increased at 4 h-8 h, followed by a drop at 12 h-24 h. While, the changes in number of leukocytes in peripheral blood and proportion of neutrophils in bone marrow have the opposite trend. Meanwhile, neutrophils released neutrophil extracellular traps (NETs) to clean zymosan particles. In contrast, the cell migration response to carrageenan increased during 4 h to 24 h, no obvious NETs were observed, and the number of leukocytes in peripheral blood increased and the proportion of neutrophils in bone marrow decreased slightly. This study indicated that although both zymosan and carrageenan are sterile irritants, the characteristics of the inflammatory response induced by each other were different. In the acute phase of inflammation, zymosan-stimulated neutrophils were mobilized, recruited, and engulfed, and then died by NETs. Carrageenan stimulated the production of cytokines/chemokines by neutrophils or macrophages, but did not lead to an obvious death by releasing NETs. Graphical Abstract
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Benke Cui,Chunguang Ren,Renlong Zhang,Yuhang Ren,Jiaxin Liu,Yanzhe Gou,Tianyu Zhao,Xue Jiang,Hongli Cui,Xiuzhi Wang,Linlin Wang
Hypoxia can prolong the healing time of wounds and oxygen delivery to the hypoxic tissue has been reported an effective strategy for promoting wound regeneration. Bacterial infections can interfere with the wound healing process, leading to poor skin regeneration and even more serious complications, which is also an urgent issue to be solved during the process of wound healing. To address the problem of delayed wound healing caused by hypoxia and bacterial infections, we fabricated a series of Chlorella -loaded hydrogels (CK) using gelatin, κ-carrageenan and I-carrageenan as matrixes, which introduced Chlorella and ampicillin conferred oxygen-producing and antimicrobial capacity to the hydrogel. The CK hydrogels possessed good mechanical and adhesive properties, as well as the capability of efficient and sustained oxygen release. The hydrogels possessed outstanding hemostatic ability, excellent blood and cell compatibility, which also owned excellent antibacterial capacity against E. coli and S. aureus . More notably, the in vivo evaluation revealed that the hydrogel can accelerate wound healing by promoting collagen deposition, which may as a promising potential wound dressing for hypoxic wound healing.
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FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Yalu Yun,Xiling Cheng,Jin Xu,Yuxi Wang,Hailan Jin,Jian Li,Lijuan Wang
In this paper, κ-Carrageenan/carboxymethyl cellulose/Ca 2+ (κC/CMC/Ca 2+ ) composite cryogels with high porosity, low density, and excellent hygroscopicity were obtained via cross-linking effect. The porosity and density of the cryogel (κC: CMC (w/w) of 7:3, 5.0 wt% Ca 2+ ) were 94.85%, and 0.067 g/cm 3 , respectively, and the moisture absorption reached 39.84% at 75% relative humidity (RH). Moreover, Clitoria ternatea anthocyanins (CTA) were introduced into κC/CMC/Ca 2+ cryogel to fabricate a porous pH-colorimetric label (κC/CMC/Ca 2+ /CTA cryogel) whose maximum moisture absorption of the cryogel was increased to 42.50%. κC/CMC/Ca 2+ /CTA cryogel (κCCCC) possessed excellent pH-color development and a quick response because it turned from blue to green within 7 s after injecting 7.50 mmol/L ammonia at 53% RH. The label with diameter of 0.75 cm could be used to declare the quality of 200 g pork due to obvious color change as the total volatile basic nitrogen (TVB-N) increased to 14.57 mg/100 g. The results showed that κCCCC would play an important role in the practical application for food quality indication.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Chao Liu,A.M. Omer,Xiao-kun Ouyang
In the present study, new composite beads based on carboxymethyl cellulose (CMC)/k-carrageenan (kC)/activated montmorillonite (AMMT) were prepared for adsorptive removal of cationic methylene blue (MB) as a dye model. The structure and morphology of the composite beads were investigated by FT-IR and SEM, while the thermal properties were tested using TGA. Factors affecting the removal percent of MB such as CMC/kC/AMMT ratios, initial MB concentration, pH medium, adsorbent dosage, solution temperature, and agitation speed were also explored. Results demonstrated that MB removal (%) exceeded 92% after 120 min using CMC/kC/AMMT (1:1:0.4 ratio) compared to 69% in case of free AMMT beads. Moreover, data obtained from isotherm studies were fitted well to Langmuir model (R 2 = 0.999), and the kinetics of adsorption followed pseudo-second order model. Finally, the composite beads showed good reusability for MB dye removal with high efficiency. Results obtained from this study suggest that the prepared composite beads could be applied effectively for removing cationic dyes from aqueous solutions.
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ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Mingxi Ma,Chao Yu,Hanyuan Liu,Xuepeng Lv,Haidong Zhu,Weiwei Tang,Gaojun Teng,Fei Xiong
Hepatocellular carcinoma (HCC) is a highly fatal malignant tumor. The clinical outcomes of vascular interventional therapy for HCC based on existing drug-eluting microspheres remain unsatisfactory. This study adopted a unique fabrication method to synthesize methacrylated carrageenan/gelatin hydrogel embolic microspheres (MCGs). The interpenetrating network formed by these two biocompatible and absorbable natural macromolecules endows MCGs with outstanding mechanical properties and vascular conformity. MCGs could be delivered through microcatheters smoothly, and they are provided in various sizes to accommodate different clinical requirements. Their in vivo degradation behavior is controlled and predictable. Due to the strong anionic nature of carrageenan, MCGs can efficiently load and sustainably release Lenvatinib, achieving the precise delivery of this antiangiogenic targeted drug in the tumor region. Animal models reveal that the microspheres block blood vessels effectively and MCGs allow for high-dose Lenvatinib to have prolonged contact with HCC. We also propose an HCC treatment strategy by combining PD-L1 inhibitors with MCGs carrying Lenvatinib. This combination therapy is safe and effective, and the additive and synergistic effects showed impressive antitumor potency and great clinical application potential.
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INFLAMMOPHARMACOLOGY
·
2025
·
IF 5.3
Xiang Meixian,Wu Songtao,Liu Minxin,Zhang Bin,Xia Xiankun,Tan Wenjing,Xiang Shijian
Iota-carrageenan oligosaccharides (iCOs), derived from marine red algae, are traditionally used as antithrombotic and anti-inflammatory agents in folk medicinal practice. Despite the prevailing emphasis on these aspects in their applications, the potential of iCOs as a prebiotic agent for gut health and its subsequent impact on intestinal disorders such as colitis remains largely unexplored. A DSS-induced colitis model was employed in C57BL/6 male mice to analyze the gut microbiota via 16S rRNA sequencing. Fecal microbiota transplantation (FMT) was used to assess the therapeutic effects of iCOs on colitis. RNA sequencing (RNA-Seq) identified pathways and genes affected by iCOs. ELISA measured inflammatory cytokines, while western blot and RT-qPCR evaluated protein and gene expressions, respectively. The iCOs increased beneficial bacteria, such as Lactobacillus , Bifidobacterium , and Akkermansia . They enhanced short-chain fatty acid production and upregulated GPR41, GPR43, and GPR109A mRNA, influencing cytokine secretion. The iCOs reduced mRNA of SPHK1 , BDKRB1 , LCN2 , and so on, potentially through PI3K-Akt pathway inhibition, and promoted tight junction protein expression. Our findings highlight the novel therapeutic potential of iCOs in colitis, indicating a multifaceted approach to treatment that includes gut microbiota modulation, intestinal barrier restoration, and the suppression of inflammatory responses. Graphical abstract
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Lihua Zhang,Yuli Ning,Yinjiao Wei,Yingying Xin,Bakht Ramin Shah,Hongtao Du,Jian Zou,Wei Xu
Konjac glucomannan (KGM) undergoes deacetylation in alkaline conditions, while κ-carrageenan (CRG) is sensitive to potassium ions. This study examines the influence of K 2 CO 3 on the mechanical properties of KGM/CRG-based camellia oil Pickering emulsion gels. Texture analysis and rheological testing revealed that the addition of K 2 CO 3 significantly enhanced the mechanical properties of emulsion gels. Texture parameters, such as the hardness, gumminess, and chewiness of the gel, were closer to those of pork back fat when the K 2 CO 3 concentration was 0.2 M. The addition of K 2 CO 3 enhance the intermolecular crosslinking within the gel and formed a more uniform and dense gel network, promoting gelation and structural stability, which were confirmed by Fourier transform infrared spectroscopy, X-ray diffraction, and microstructural analysis. Besides, low-field nuclear magnetic resonance and thermogravimetric analyses indicated that the addition of K 2 CO 3 reduced the water mobility of the gel and improved its thermal stability. Physicochemical and color analysis results showed that the energy value of the camellia oil Pickering emulsion gel was only 15.9 % that of pork back fat, and its appearance was closer to that of pork back fat than hydrogels. This study provides technical support for the use of KGM/CRG-based camellia oil Pickering emulsion gels as fat analogs.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiaofan Zhang,Yuli Ning,Liwen Chai,Yongpeng Yin,Denglin Luo,Wei Xu
This study aimed to elaborate the combination effect of polysaccharides on physicochemical properties and in vitro digestive behavior of astaxanthin (AST)-loaded Pickering emulsion gel. AST-loaded Pickering emulsion gel was prepared by heating Pickering emulsion with konjac glucomannan (KGM) and κ-carrageenan (CRG). The microstructure revealed that adding the two polysaccharides resulted in Pickering emulsion forming a network structure. It exhibited a denser and more uniform network structure, enhancing its mechanical properties four times and increasing its water-holding capacity by 20 %. In vitro digestion experiments demonstrated that the release of free fatty acids from the Pickering emulsion gel (4.25 %) was notably lower than that from conventional Pickering emulsion (17.19 %), whereas AST bioaccessibility was remarkably low at 0.003 %. It provided a feasible strategy to regulate the bioaccessibility in Pickering emulsion, which has theoretical significance to guide the current eutrophic diet people.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Ting Zhuo,Jun-ru Qi,Jin-song Liao,Hui-ying Wei,Rong Xiao
Konjac glucomannan (KGM) is a natural functional polysaccharide, and deacetylation (removing acetyl groups) impacts its physicochemical and gel properties. In order to study KGM's gel properties and explore new deacetylation modifiers, three deacetylated konjac glucomannan (DKGM) with different deacetylation degrees (DD, 17.51 %, 35.41 %, and 54.52 %) were prepared using L-arginine. Results showed that L-arginine deacetylated and retained the main structure and high molecular weight of KGM. Solubility decreased from 85.38 % to 81.17 %, transparency decreased from 38.52 % to 35.73 %, water absorbing and swelling slowed down, and molecular morphology changed from chain structures to micelle-like aggregates after deacetylation. Dynamic viscoelastic analysis showed that deacetylation facilitated the formation of the entangled network structure of KGM. The strength of konjac glucomannan and κ-carrageenan mixed gels increased after deacetylation, and this enhancement was mainly through hydrogen bonds and hydrophobic interactions. These findings will provide theoretical guidance for the application of KGM.
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高影响因子
Frontiers of Environmental Science & Engineering
·
2022
·Vol. 16
·期 10
·1-14
·
IF 6.048
Li Na,Gao Boqiang,Yang Ran,Yang Hu
3D composite aerogels (CMC-CG) composed of carboxymethyl cellulose and κ-carrageenan were designed and fabricated using the one-pot synthesis technique. The optimized CMC-CG showed a good mechanical property and a high swelling ratio due to its superior textural properties with a proper chemically cross-linked interpenetrating network structure. CMC-CG was utilized for the removal of various fluoroquinolones (FQs) from water and exhibited high adsorption performance because of effective electrostatic attraction and hydrogen bonding interactions. Ciprofloxacin (CIP), a popular FQ, was used as the representative. The optimized CMC-CG had a theoretically maximal CIP uptake of approximately 1.271 mmol/g at the pH of 5.0. The adsorption capacity of CMC-CG was improved in the presence of some cations, Cu 2+ and Fe 3+ ions, at a low concentration through the bridging effect but was reduced at a high concentration. The investigation of adsorption mechanisms, based on the adsorption kinetics, isotherms and thermodynamic study, Fourier transform infrared spectrometry and X-ray photoelectron spectroscopy analyses before and after adsorption, and changes in the adsorption performance of CMC-CG toward two molecular probes, further indicated that electrostatic attraction was the dominant interaction rather than hydrogen bonding in this adsorption. CMC-CG after saturated adsorption of CIP could be easily regenerated using a dilute NaCl aqueous solution and reused efficiently. Moreover, the disused aerogel could still be reused as a new adsorbent for effective adsorption of Cu 2+ ion. Overall, this study suggested the promising applications of this composite aerogel as an eco-friendly, cost-effective, and recyclable adsorbent for the efficient removal of FQs from water.
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