JOURNAL OF CONTROLLED RELEASE
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2020
·
IF 7.727
Zhihao Guo,Junhui Sui,Mengcheng Ma,Jianshe Hu,Yong Sun,Liqun Yang,Yujiang Fan,Xingdong Zhang
Stimuli-responsive nanotechnology-mediated drug co-delivery system is a notable strategy to improve access of the systemically administered chemotherapeutics to the tumors. Herein, a tailor-made 2,3-dimethylmaleic-anhydride-poly(ethylene glycol)- ε -poly- l -lysine-doxorubicin /lapatinib polymeric nanoplatform (DMMA-P-DOX/LAP) for synergistically eliminating breast cancer is developed by encapsulating lapatinib into dual-pH responsive charge switchable biopolymer-doxorubicin conjugate nanoparticles . The physicochemical properties of polymeric nanoparticles are conducive to their stable circulation in the physiological condition, but reverse the surface charge from negative to positive ultrasensitively in slightly acidic tumor microenvironment , facilitating cell internalization and deep tumor penetration. Subsequently, DOX and LAP are synchronously released into the cytoplasm in response to the significantly increased acidity of intracellular environment. As a result, the combination therapy by DMMA-P-DOX/LAP nanoparticles compels the solid tumors to contract significantly or even vanish completely in the MCF-7 tumor model, moreover, the structural composition with amino acid and bioinert PEG ensures the favorable biosecurity of the co-delivery system in vivo . This dual-pH responsive nanotechnology-mediated drug co-delivery system provides great potentials for safe and effective cancer therapy.
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PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2019
·
IF 4.527
Yang Wu,Yan Li,Liangqing Zheng,Pengcheng Wang,Yan liu,Yongning Wu,ZhiYong Gong
Nε-(carboxymethyl)lysine (CML) is a potentially noxious compound that is causing widespread concern due to its use in various food products. In this study, we investigated CML neurotoxicity via an in vivo experiment with mice, and an in vitro experiment using a 3D microvascular network model (with human brain vascular endothelial cell and human astrocyte) that simulated the blood-brain barrier. We found that CML could induce cell survival status variations, and histopathological changes to the brain. In addition, CML increased levels of oxidative stress , prompted the protein expression of the receptor for advanced glycation end-products (RAGE). CML up-regulated both the gene expression of RAGE, the activating protein-1 (AP-1), the inflammatory cytokines Interleukin-6 (IL-6), vascular cell adhesion molecule1 (VCAM-1), monocyte chemotactic protein1 (MCP-1). We, therefore, postulated that CML has the potential to deleteriously affect the nervous system through oxidative stress and that activation of the p38 MAPK-AP-1 signaling pathway might be implicated in this pathological process.
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高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 26
·4314-4325
·
IF 4.776
Tao Liu,Shaohua Chen,Siyi Zhang,Xidong Wu,Peina Wu,Beiping Miao,Xiang Cai
Transferrin-functionalized chitosan-graft-poly(L-lysine) dendrons (CS–PLLD–Tf) were synthesized in this work and then used to deliver the MMP-9 shRNA plasmid to HNE-1 cells for gene therapy. The obtained CS–PLLD–Tf displayed excellent gene transfection in vitro, verifying the strategy of constructing a high-efficiency gene carrier by conjugating the multiple low generation PLLDs to one molecule of CS to form a copolymer. Particularly, CS–PLLD–Tf showed much higher gene transfection efficiency than PEI-25k and CS–PLLD in the presence of serum, and more than 40% HNE-1 cells were transfected. This result led to an obvious decrease in MMP-9 protein expression and the apoptosis of HNE-1 cells as well as inhibition of the growth and invasion of HNE-1 tumors. The in vivo anti-tumor assay showed that CS–PLLD–Tf delivered the MMP-9 plasmid effectively and the tumor volume decreased more than 77% compared with the PBS control group. Moreover, CS–PLLD–Tf displayed higher bioavailability and good biocompatibility such as lower cytotoxicity, excellent blood compatibility and non-toxicity to organs, suggesting a potential application in gene therapy of tumors.
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高影响因子
RSC Advances
·
2017
·Vol. 7
·期 29
·17711-17719
·
IF 3.108
Yingling Miao,Ruirui Yang,David Y. B. Deng,Li-Ming Zhang
Excellent biocompatibility and bioactivity are necessary requirements for a scaffold for nerve repair and regeneration. Natural plant protein zein was chosen as the primary material and poly(L-lysine), which is composed of common amino acids in the human body, was used to modify it. Poly(L-lysine) modified zein (ZPLL) with different PLL contents of 1.46%, 3.57%, and 6.18% was synthesized and nanofibrous membranes were prepared by electrospinning. The hydrophilicity of the membranes improved with an increase of PLL content. The biodegradability of the membranes was proved by in vitro experiments. Compared with pure zein membranes, ZPLL membranes can efficiently improve cell viability, adhesion, proliferation, and differentiation of neural stem cells (NSCs) and the effect of PLL content was further investigated. The results show that when the PLL content was 3.57%, cell adhesion and proliferation proved to be the best and most differentiated toward mature neurons with extensive neurite formation and astrocytes rather than oligodendrocytes. The ZPLL was made into nerve conduits for future study and they may be a promising biomaterial for nerve repair and regeneration.
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高影响因子
Frontiers in Bioengineering and Biotechnology
·
2024
·
IF 4.3
You Xiaoxiao,Wang Zhongke,Wang Li,Liu Youbo,Chen Hongmei,Lan Xiaorong,Guo Ling
The construction of an antibacterial biological coating on titanium surface plays an important role in the long-term stability of oral implant restoration. Graphene oxide (GO) has been widely studied because of its excellent antibacterial properties and osteogenic activity. However, striking a balance between its biological toxicity and antibacterial properties remains a significant challenge with GO. ε-poly-L-lysine (PLL) has broad-spectrum antibacterial activity and ultra-high safety performance. Using Layer-by-layer self-assembly technology (LBL), different layers of PLL/GO coatings and GO self-assembly coatings were assembled on the surface of titanium sheet. The materials were characterized using scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and contact angle test. The antibacterial properties of Porphyromonas gingivalis (P.g.) were analyzed through SEM, coated plate experiment, and inhibition zone experiment. CCK-8 was used to determine the cytotoxicity of the material to MC3T3 cells, and zebrafish larvae and embryos were used to determine the developmental toxicity and inflammatory effects of the material. The results show that the combined assembly of 20 layers of GO and PLL exhibits good antibacterial properties and no biological toxicity, suggesting a potential application for a titanium-based implant modification scheme.
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高影响因子
Advanced Science
·
2024
·
IF 14.3
Xianchi Zhou,Wenzhong Cao,Yongcheng Chen,Zihao Zhu,Yifeng Chen,Yanwen Ni,Zuolong Liu,Fan Jia,Zhouyu Lu,Yang Ye,Haijie Han,Ke Yao,Weifeng Liu,Xinyue Wei,Shengfu Chen,Youxiang Wang,Jian Ji,Peng Zhang
The foreign body response (FBR) to implanted biomaterials and biomedical devices can severely impede their functionality and even lead to failure. The discovery of effective anti-FBR materials remains a formidable challenge. Inspire by the enrichment of glutamic acid (E) and lysine (K) residues on human protein surfaces, a class of zwitterionic polypeptide (ZIP) hydrogels with alternating E and K sequences to mitigate the FBR is prepared. When subcutaneously implanted, the ZIP hydrogels caused minimal inflammation after 2 weeks and no obvious collagen capsulation after 6 months in mice. Importantly, these hydrogels effectively resisted the FBR in non-human primate models for at least 2 months. In addition, the enzymatic degradability of the gel can be controlled by adjusting the crosslinking degree or the optical isomerism of amino acid monomers. The long-term FBR resistance and controlled degradability of ZIP hydrogels open up new possibilities for a broad range of biomedical applications.
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高影响因子
PHYTOMEDICINE
·
2024
·
IF 6.7
Fukui Shen,Wen Yang,Guoqing Luan,Jiamin Peng,Zhenqiang Li,Jie Gao,Yuanyuan Hou,Gang Bai
Background Catalpol (CAT), a naturally occurring iridoid glycoside sourced from the root of Rehmannia glutinosa , affects mitochondrial metabolic functions. However, the mechanism of action of CAT against pyrexia and its plausible targets remain to be fully elucidated. Purpose This study aimed to identify the specific targets of CAT for blocking mitochondrial thermogenesis and to unveil the unique biological mechanism of action of the orthogonal binding mode between the hemiacetal group and lysine residue on the target protein in vivo . Methods Lipopolysaccharide (LPS)/ carbonyl cyanide 3-chlorophenylhydrazone (CCCP)-induced fever models were established to evaluate the potential antipyretic effects of CAT. An alkenyl-modified CAT probe was designed to identify and capture potential targets. Binding capacity was tested using in-gel imaging and a cellular thermal shift assay. The underlying antipyretic mechanisms were explored using biochemical and molecular biological methods. Catalpolaglycone (CA) was coupled with protein profile identification and molecular docking analysis to evaluate and identify its binding mode to UCP2. Results After deglycation of CAT in vivo , the hemiacetal group in CA covalently binds to Lys239 of UCP2 in the mitochondria of the liver via an ɛ-amine nucleophilic addition. This irreversible binding affects proton leakage and improves mitochondrial membrane potential and ADP/ATP transformation efficiency, leading to an antipyretic effect. Conclusion Our findings highlight the potential role of CA in modulating UCP2 activity or function within the mitochondria and open new avenues for investigating the therapeutic effects of CA on mitochondrial homeostasis.
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高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Duo Xu,Xinyang Sun,Simiao Wu
Forward osmosis (FO) technology has been employed extensively in a multitude of fields. The primary impediment to the development of this technology is the phenomenon of bio-fouling. This study presented an innovative and straightforward approach to mitigating bio-fouling and simultaneously improving water drawing performance through the incorporation of arginine (Arg) and lysine (Lys) into the active layer of FO membranes. This was contributed from the hydrophilic groups (i.e. guanidine and amino groups) derived from Arg and Lys. The highest water fluxes were observed of 23.3 ± 0.4 and 20.7 ± 0.7 LMH, with Arg and Lys loading concentrations of 6 and 7 wt%, respectively (Arg-FO-6 and Lys-FO-7 membrane). The E. coli fouling test demonstrated that the modified FO membranes exhibited excellent and stable antibacterial activity. The normalized water fluxes of the Arg-FO-6 and Lys-FO-7 membranes remained at 0.50 and 0.59, respectively, after 120-hour dynamic fouling, whereas the flux of the pristine membrane declined to 0.32. The Arg-FO-6 and Lys-FO-7 membranes exhibited total membrane resistance ( R t ) of 3.6 × 10 8 and 4.9 × 10 8 m −1 , respectively, significantly lower than the pristine membrane (11.4 × 10 8 m −1 ). These findings demonstrate that the modification of FO membranes with Arg and Lys is an effective approach with significant potential for alleviating bio-fouling.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xinzong Li,Hongfeng Gao,Yujun Li,Yonggang Wang,Haiyong Zhang
A novel type of humic acid-based nitrogen-doped graphene quantum dots (N-GQDs) exhibiting strong blue fluorescence emission was synthesized using a straightforward hydrothermal method, with coal-derived humic acid and ethanolamine serving as precursors. It was observed that the incorporation of Fe 3+ ions quenches the fluorescence emission of the N-GQDs, the classification of quenching is recognized as static quenching; however, the addition of l -lysine to the N-GQDs/Fe 3+ system effectively restores this fluorescence. Building on these findings, this study further investigates the potential application of N-GQDs as an “on-off-on” fluorescent sensor for detecting both Fe 3+ and l -lysine. It has been established that N-GQDs exhibit commendable selectivity and sensitivity towards Fe 3+ , achieving a detection limit (LoD) of 0.41 μM. Furthermore, the stable N-GQDs/Fe 3+ complex demonstrates significant detection sensitivity to l -lysine in aqueous environments, with a LoD of 1.06 μM. The experimental results indicate that the synthesized N-GQDs possess favorable properties including excellent fluorescence intensity, dispersibility, and water solubility. The developed sensor exhibits considerable potential for improvement and holds promising applications in the domain of water quality monitoring. Additionally, the fluorescence selectivity of N-GQDs towards Fe 3+ was elucidated through calculations involving frontier molecular orbitals.
研究领域:
分析化学
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环境监测
·
纳米材料
·
生物传感
·
水处理
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分子探针
·
荧光分析
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高影响因子
Water
·
2025
·Vol. 17
·期 22
·3234
·
IF 3
Zeyu Xiong,Peng Jing
Road-marking operations generate alkaline wash water with intense color and soluble cationic additives. A new biomass adsorption material (LML) was developed to address dye pollution in road-marking wash water effectively. Enzymatically hydrolyzed lignin was used as the raw material for the first time. L-lysine was modified to the structure of the lignin benzene ring using a simple one-step synthesis method, which endowed lignin with a large number of active carboxyl and amino functional groups to improve its adsorption capacity. The adsorption performance of LML for methylene blue in water was also investigated. The experimental results show that the LML has a high dye removal rate under alkaline conditions. The fitted adsorption model shows that the saturated adsorption capacity of LML for methylene blue (MB) is 129.4 mg g−1and malachite green (MG) is 244.9 mg g−1, which is in line with the Langmuir isotherm adsorption model. The adsorption process is endothermic, which means that the adsorption capacity increases with increasing temperature. Kinetic studies showed that the adsorption process reached equilibrium within 120 min following a pseudo-second-order kinetic model. The cycle experiment shows that the removal efficiency of the adsorbent for dyes can still reach 90% after five cycles, indicating a good practical application value for the polishing of road-marking wash water.
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高影响因子
Carbohydrate Polymer Technologies and Applications
·
2021
·
IF 0
Yiru Wang,Wenrong Qin,Qi Liang,Feng Zhou,Chengyun Yan,Yueyi Deng
The purpose of this study was to enhance quercetin (Que) dissolution by preparing its ternary amorphous solid dispersions. The ternary amorphous solid dispersions were prepared by solvent evaporation method using hydroxypropylmethylcellulose acetate succinate (HPMCAS) and L-lysine (lys) as the carriers. The effect of the combination of polymer and amino acid on nucleation inhibition was evaluated by the nucleation induction time. Supersaturation tests were used to investigate the ability of maintaining Que supersaturation of the combination of polymer and lys. The dissolution results showed that the dissolution effect of ternary solid dispersions was better than binary solid dispersions. Stronger crystallization inhibition didn't lead to better dissolution effect and the dissolution of the prepared amorphous solid dispersions of Que was carrier-controlled. Lys modified the dissolution microenvironment of the solid dispersions, which made drug dissolution in the optimal pH environment, and effectively improved drug dissolution. Polymer/lys can be one of the most potential carriers for preparing amorphous solid dispersions of Que.
研究领域:
药剂学
·
药物化学
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DOI
CRYSTENGCOMM
·
2022
·Vol. 24
·期 36
·6400-6407
·
IF 3.756
Anzhou Yang,Qiuzi Huang,Ziqi Wei,Zehan Yu,Meifeng Cui,Wu Lei,Yawen Tang,Xiaoyu Qiu
Designing cost-effective, highly active, and durable noble-metal-free electrocatalysts as alternative electrode materials for the methanol oxidation reaction (MOR) is of significant importance for practical applications. Herein, we report a facile one-pot synthetic route to construct Cu@Ni core–satellite nanoassemblies (CS-NAs) with the assistance of eco-friendly L-lysine, which involves the spontaneous process of preferential formation of the Cu core followed by self-assembly growth of Ni satellites. The selection of the Cu core is beneficial for enhancing the charge transfer and tuning the electronic structure of Ni satellites, as well as serving as a self-skeleton to enhance the structural stability for catalysis. Meanwhile, the tiny Ni satellites could be fully exposed, which not only produce a larger number of valid active sites, but also provide a short electron and ion transport distance. As a result, the Cu@Ni CS-NAs are qualified as superior and durable electrocatalysts for the MOR, affording 29.6 mA cm−2 at 1.6 V with long-term chronoamperometric stability.
研究领域:
材料科学
·
电化学
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危险品化学品经营许可证（带存储）