CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2017
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IF 0.795
Dan-Dan TANG,Jin-Yi ZHANG,Xian-Deng HOU,Peng WU
The key to maximize the sensitivity of inner filter effect (IFE)-based assay is to enlarge the overlap between the absorption spectra of the absorber and the excitation and/or emission bands of the fluorophore. In this work, Mn-doped ZnS QDs is chosen for IFE-based detection of β -glucuronidase (GUS), since the excitation spectrum of QDs is perfectly overlapped with the absorption spectrum of the substrate of GUS, namely 4-nitrophenyl- β-D- glucuronide (PNPG). Since absorption spectrum of the enzymatic product of GUS-PNP are almost not overlapped with the excitation and emission spectra of Mn-ZnS QDs, the phosphorescent turn-on determination of GUS can be successfully proposed and carried out. In addition, the excellent room temperature phosphorescence property of Mn-ZnS QDs can eliminate the fluorescence background from biological organisms, which can be effectively applied to biological sample analysis with good anti-interference ability. Accordingly, a simple turn-on phosphorescent assay of GUS was developed with a linear range of 10–300 U L −1 and a limit of detection of 7 U L −1 , and the proposed system was also successfully applied for actual detection of Escherichia coli .
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POWDER TECHNOLOGY
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2018
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IF 3.23
Weiwei Chu,Zhenzhen Lu,Ruiqin Tan,Shihui Tang,Wei Xu,Weijie Song,Junhua Zhao
In this work, β-SrHPO 4 , Sr 3 (PO 4 ) 2 , and Sr 5 (PO 4 ) 3 (OH) (SrHAp) powders were successfully prepared by a controllable hydrothermal method and were utilized in removal of lead ion (Pb 2+ ). The effects of pH value, contact time and initial ion concentration on the immobilization of Pb 2+ were investigated systematically. Results showed that β-SrHPO 4 exhibited a capacity of 1438 ± 45 mg/g for Pb 2+ , while Sr 3 (PO 4 ) 2 and SrHAp showed values of 1223 ± 45 mg/g and 543 ± 45 mg/g respectively under the optimum conditions. The XRD , SEM and EDX results revealed that the mechanism of Pb 2+ immobilization by β-SrHPO 4 , Sr 3 (PO 4 ) 2 and SrHAp was mainly due to a process of dissolution and precipitation in the acidic solution. Comparing the immobilization behavior and capacities of β-SrHPO 4 , Sr 3 (PO 4 ) 2 and SrHAp for Pb 2+ removal, it is shown that β-SrHPO 4 is an effective and eco-friendly material for the removal of Pb 2+ from acidic aqueous solutions among strontium phosphates materials.
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Scientific Reports
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2018
·Vol. 8
·期 1
·1-11
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IF 4.122
Jiang Zhiqiang,Dong Xiaoyan,Yan Xin,Liu Yang,Zhang Lin,Sun Yan
Inhibition of amyloid β-protein (Aβ) aggregation is considered as a promising strategy for the prevention and treatment of Alzheimer’s disease. Epigallocatechin-3-gallate (EGCG) and curcumin have been recognized as effective inhibitors of Aβ aggregation. Herein, we proposed dual-inhibitor modification of hyaluronic acid (HA) to explore the synergistic effect of the two inhibitors. EGCG-modified HA (EHA) formed dispersed hydrogel structures, while EGCG-curcumin bi-modified HA (CEHA) self-assembled into nanogels like curcumin-modified HA (CHA). Thioflavin T fluorescent assays revealed that the inhibitory effect of CEHA was 69% and 55% higher than EHA and CHA, respectively, and cytotoxicity assays showed that the viability of SH-SY5Y cells incubated with Aβ and CEHA was 28% higher than that with Aβ and the mixture of EHA and CHA. These results clearly indicate the synergism of the two inhibitors. It is considered that the difference in the hydrophobicities of the two inhibitors made the bi-modification of HA provide a favorable CEHA nanostructure that coordinated different inhibition effects of the two inhibitors. This research indicates that fabrication of dual-inhibitor nanosystem is promising for the development of potent agents against Aβ aggregation and cytotoxicity.
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高影响因子
Scientific Reports
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2018
·Vol. 8
·期 1
·1-18
·
IF 4.122
Zhou Bohang,Zhang Bingyu,Li Xingqiang,Liu Xiuxiu,Li Hui,Li Ding,Cui Zhiming,Geng Huiling,Zhou Le
A series of 2-aryl-9-methyl- β -carbolinium bromides (B) were synthesized and explored for anti-acetylcholinesterase (AChE) activities in vitro , action mechanism and structure-activity relationship. All the compounds B along with their respective 3,4-dihydro intermediates (A) presented anti-AChE activity at 10 μM. Thirteen compounds B showed the excellent activity with IC 50 values of 0.11 – 0.76 μM and high selectivity toward AChE relative to butyrylcholinesterase (BChE), superior to galantamine (IC 50 = 0.79 μM), a selective AChE inhibitor drug. Kinetic analysis showed that the action mechanisms of both compounds B and A are a competitive inhibition model. Structure-activity relationship analyses showed that the C = N + moiety is a determinant for the activity. Substituents at 6, 7 or 4′ site, the indole-N-alkyl and the aromatization of the C-ring can significantly improve the activity. Molecular docking studies showed that the compounds could combine with the active site of AChE by the π-π or cation-π action between the carboline ring and the phenyl rings of the residues, and the β-carboline moiety is embedded in a cavity surrounded by four aromatic residues of Trp86, Tyr337, Trp439 and Tyr449. The present results strongly suggest that the para-position of the D-ring should be a preferred modification site for further structural optimization design. Thus, 2-aryl-9-methyl- β -carboliniums emerged as novel and promising tool compounds for the development of new AChE inhibitor agents.
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JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Mingtao Tan,Hong Jiang,Rusong Chai,Miao Fan,Zengting Niu,Guotong Sun,Shanchun Yan,Dun Jiang
Heavy metal pollutants are important abiotic environmental factors affecting pest habitats. In this study, Cd pre-exposure significantly increased the tolerance of Lymantria dispar larvae to β-cypermethrin, but did not significantly alter their tolerance to λ-cyhalothrin and bifenthrin. The activation of P450 by Cd exposure is the key mechanism that induces insecticide cross-tolerance in L. dispar larvae. Both before and after β-cypermethrin treatment, Cd exposure significantly increased the expression of CYP6AB224 and CYP6AB226 in L. dispar larvae. Silencing CYP6AB224 and CYP6AB226 reduced the tolerance of Cd-treated L. dispar larvae to β-cypermethrin. Transgenic CYP6AB224 and CYP6AB226 genes significantly increased the tolerance of Drosophila and Sf 9 cells to β-cypermethrin, and the recombinant proteins of both genes could significantly metabolise β-cypermethrin. Cd exposure significantly increased the expression of CnCC and Maf . CnCC was found to be a key transcription factor regulating CYP6AB224 - and CYP6AB226 -activated insecticide cross-tolerance in Cd-treated larvae. Decreasing reactive oxygen species (ROS) levels in the Cd-treated larvae or increasing ROS levels in the untreated larvae reduced or enhanced the expression of CnCC , CYP6AB224 and CYP6AB226 and β-cypermethrin tolerance in L. dispar larvae, respectively. Collectively, Cd exposure confers β-cypermethrin tolerance in L. dispar larvae through the ROS/ CnCC signalling pathway-mediated P450 detoxification. Environmental Implication Heavy metals have been reported to produce cross-tolerance in agricultural insects to insecticides. The growing demand for chemical pest control necessitates expanding research on the widespread occurrence of cross-tolerance to insecticides among pests, particularly forest pests, in areas polluted with heavy metals. In this study, Cd pre-exposure enhanced the cross-tolerance of Lymantria dispar larvae to β-cypermethrin. Cd pre-exposure activated the P450 detoxification system of L. dispar larvae. CYP6AB224 and CYP6AB226 were key detoxify genes to the formation of cross-tolerance. ROS-CnCC signaling pathway was proved to be responsible for activation of detoxify genes.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Md Alomgir Hossen,Islam Md Shimul,Dur E. Sameen,Zainab Rasheed,Wuxia Tanga,Mingrui Chen,Yaowen Liu
Blueberries are highly susceptible to fungal pathogens and oxidative deterioration due to their thin epidermal layer, which can be mitigated by applying a natural polymer-based antimicrobial coating to their surface. This study aimed to develop a chitosan/gelatin-based antimicrobial coating utilizing ginger essential oil (GEO) to extend the postharvest quality of blueberries. To ensure GEO's stability within the coating, it was initially encapsulated with β-cyclodextrin (β-CD) using the inclusion complexation technique. The GEO/β-CD inclusion complex (IC) formed rhomboidal shapes with high encapsulation efficiency and small particle sizes. When the optimized GEO/β-CD IC was incorporated into the chitosan/gelatin polymer solution, it significantly increased surface hydrophobicity and free radical scavenging activity, and suppressed the growth of three selected fungi, namely Botrytis cinerea, Penicillium italicum and Alternaria alternaria . The results of postharvest storage quality revealed that blueberry samples coated with CH/Gel-GEO/β-CD IC-5 effectively maintained the quality of blueberries by decreasing weight loss and decay incidence, and regulating anthocyanin and other oxidation-related enzyme activities compared to the control group during 16 days at 25 °C and 40 days at 4 °C storages. In conclusion, it can be stated that CH/Gel-GEO/β-CD composite coating can be a promising technology to address the drawbacks of blueberry preservation.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Xuewu Liu,Xingfei Li,Zhengjun Xie,Xing Zhou,Long Chen,Chao Qiu,Cheng Lu,Zhengyu Jin,Jie Long
Agarose from biomass can be used to synthesize the rare sugar 3,6-anhydro- L -galactose ( L -AHG), and the new synthesis route and functional properties of L -AHG have always been the focus of research. Here we developed a novel method to co-immobilize Aga50D and Bp GH117 onto streptavidin-coated magnetic nanoparticles and achieved the conversion of agarose to bioactive L -AHG in one pot. Results showed that enzymes were successfully immobilized on the carrier. The activity of co-immobilized enzymes was 2.5-fold higher than that of single immobilized enzymes. Compared with free enzymes, co-immobilized enzymes exhibited enhanced thermal stability. The co-immobilized enzymes retained 79.45 % relative activity at 40 °C for 3 h, while the free enzymes only possessed 21.40 % residual activity. After eight cycles, the co-immobilized enzymes still retained 73.47 % of the initial activity. After silica gel chromatography, the purity of L -AHG obtained by co-immobilized enzymes hydrolysis reached 83.02 %. Furthermore, bioactivity experiments demonstrated that L -AHG displayed better antioxidant and antibacterial effects than neoagarobiose. L -AHG had broad-spectrum antibacterial activity, while neoagarobiose and D -galactose did not show an obvious antibacterial effect. This study provides a feasible method for the production of L -AHG by a co-immobilized multi-enzyme system and confirms that L -AHG plays a key role in the bioactivity of neoagarobiose.
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高影响因子
BIOMATERIALS
·
2024
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IF 12.8
Xiudan Wang,Rui Tian,Chenghong Liang,Yifan Jia,Lingyun Zhao,Qinyuan Xie,Fenglian Huang,Huijuan Yuan
Insulin resistance and pancreatic β -cell dysfunction are the main pathogenesis of type 2 diabetes mellitus (T2DM). However, insulin therapy and diabetes medications do not effectively solve the two problems simultaneously. In this study, a biomimetic oral hydrogen nanogenerator that leverages the benefits of edible plant-derived exosomes and hydrogen therapy was constructed to overcome this dilemma by modulating gut microbiota and ameliorating oxidative stress and inflammatory responses. Hollow mesoporous silica (HMS) nanoparticles encapsulating ammonia borane (A) were used to overcome the inefficiency of H 2 delivery in traditional hydrogen therapy, and exosomes originating from ginger (GE) were employed to enhance biocompatibility and regulate intestinal flora. Our study showed that HMS/A@GE not only considerably ameliorated insulin resistance and liver steatosis, but inhibited the dedifferentiation of islet β -cell and enhanced pancreatic β -cell proportion in T2DM model mice. In addition to its antioxidant and anti-inflammatory effects, HMS/A@GE augmented the abundance of Lactobacilli spp. and tryptophan metabolites, such as indole and indole acetic acid, which further activated the AhR/IL-22 pathway to improve intestinal-barrier function and metabolic impairments. This study offers a potentially viable strategy for addressing the current limitations of diabetes treatment by integrating gut-microbiota remodelling with antioxidant therapies.
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CARBOHYDRATE POLYMERS
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2024
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IF 10.7
Xi Wang,Jingjing Mu,Junjie Ren,Hairong Yu,Xingbin Lv,Ting Liang,Changjing Cheng
Cholesterol (CHO) is an essential lipid in cell membranes and a precursor for vital living substances. Abnormal CHO levels can cause cardiovascular diseases. Therefore, simple and accurate monitoring of CHO levels is crucial for early diagnosis and effective management of cardiovascular diseases. Herein, we report a β -cyclodextrin-based supramolecular photonic crystal hydrogel (PCH) biosensor for naked-eye visual CHO detection. This sensor is composed of a poly(acrylamide- co - N -isopropylacrylamide- co -maleic anhydride- β -cyclodextrin) smart hydrogel with embedding Fe 3 O 4 colloidal chains, which can translate CHO concentration signals into visually detectable color changes of the hydrogel. By carefully selecting a macromolecular crosslinker, a macroporous structure of the hydrogel was achieved, significantly enhancing CHO responsiveness. Molecular docking provides detailed information about the binding mode between MAH- β -CD and CHO. The cyclohexanol portion and alkyl chain of CHO molecule are inserted into MAH- β -CD cavity, driven primarily by hydrophobic interactions. Moreover, this sensor demonstrates excellent regenerability and could be facilely regenerated by washing using hot/cold water or acid solution. Furthermore, it is highly selective for CHO and is suitable for detecting actual human serums. Such a β -CD-based supramolecular PCH biosensor with visualization, excellent regenerability, and high sensitivity and selectivity toward CHO, provides a basis for clinical diagnosis of diseases relative to CHO abnormalities.
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CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Yue Xiong,Chao He,Xun Lin,Ke Cheng,Fumei He,Jingxin Zhao,Mengjie Yang,Hong Gao,Fangjie He,Xiaopei Zhang,Zeqi Liu,Gan Liu,Wenbin Deng
Black phosphorus nanosheet (BPNS) has recently demonstrated intrinsic anti-tumor bioactivity, but its underlying molecular mechanism remains unclear, limiting its potential applications in biomedicine. In this study, we investigated the impact of BPNS on glioblastoma cells and observed a significant dose-dependent inhibition of invasion and migration. RNA sequencing analysis revealed downregulation of genes associated with the WNT/β-catenin and NOTCH signaling pathways, both linked to invasion and migration. Mechanistically, BPNS directly binds to CSNK2A2, reducing its kinase activity, which indirectly enhances GSK-3β kinase activity. As a result, GSK-3β increases the phosphorylation level of β-catenin, leading to its degradation and subsequent inhibition of downstream molecules in the WNT/β-catenin signaling pathway. Our study uncovers the inherent biological activity of BPNS in hindering glioblastoma invasion and migration and sheds light on the molecular mechanisms, offering new directions for the nanomaterial’s biomedical applications.
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INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
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2024
·Vol. 77
·期 3
·961-972
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IF 2.5
Shujie Cheng,Shimin Wu
This study aimed to examine the impact of β-carotene, astaxanthin and phytosterol esters on the colour and oxidative stability of butter, as well as to explore a shelf life prediction method based on colour analysis. The results of correlation analysis indicated a significant linear correlation between acidity and whiteness index and yellowness index, with a linear fit coefficient ( r 2 ) exceeding 0.9796. In comparison with traditional shelf life prediction models, this method exhibited an average deviation of only 6.9% in its predicted results. The findings may contribute to the rapid evaluation of the shelf lives of commercial butter products.
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CHEMICAL ENGINEERING JOURNAL
·
2024
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IF 13.3
Shen Wang,Meng Rong,Lin-Bing Sun,Yunze Zhao,Huifang Xing,Liangrong Yang
Indeed, the efficient and sustainable recovery of lithium from highly concentrated sodium-containing mother liquors of Li 2 CO 3 and salt-lake brines remains a significant challenge. Herein, two typical Li + recognition receptors, namely benzo-12-crown-4 and thenoyltrifluoroacetone, were separately introduced into the cationic and anionic parts to successfully synthesize novel bi-functionalized task-specific ionic liquids [(B12C4)C n im][TTA] (n = 4, 6, 8). A new, green, and high-efficiency extraction system has been developed by dissolving [(B12C4)C 6 im][TTA] in commercial room-temperature ionic liquid 1-hexyl-3-methyl-imdazolium bis(trifluromethylsulfonyl)imide [C 6 mim][NTf 2 ], without the addition of any other co-extractants. The newly developed [(B12C4)C 6 im][TTA]-[C 6 mim][NTf 2 ] extraction system exhibited a wide pH working range (4.0–11.0), high lithium extraction efficiency (95.4 %), as well as superior separation factors of Li/Na (β Li/Na = 2154.0) and Li/K (β Li/K =757.2). The extraction mechanism involving cation exchange was determined through slope analysis, ion chromatography, UV–vis spectroscopy and FT-IR spectra measurement, indicating that one [(B12C4)C 6 im][TTA] molecule can interact with two lithium ions and form a 1:2 complex. Moreover, the [(B12C4)C 6 im][TTA]-[C 6 mim][NTf 2 ] extraction system also demonstrated fast extraction kinetics, facile stripping and regeneration, along with good cycling performance. Furthermore, a multi-stage countercurrent extraction process to extract lithium from the simulated mother liquor of Li 2 CO 3 was evaluated. When the volume ratio (O/A) of oil phase (O) to water phase (A) was 1.0, only two theoretical extraction stages were needed to extract 91.4 % of lithium into the organic phase. Thus, the [(B12C4)C 6 im][TTA]-[C 6 mim][NTf 2 ] extraction system is promising for lithium extraction from mother liquor of Li 2 CO 3 and salt-lake brines. This study also opens the avenue for designing cationic and anionic bi-functionalized ionic liquids for metal ion separation.
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