Journal of Functional Foods
·
2026
·
IF 3.9
Jialing Yu,Jiayao Li,Zhufang Duan,Dandan Han,Yingrui Cao,Gang Wu,Xin Lü,Fang Xie,Guoqiang Qian,Yanglei Yi
Goji berries ( Lycium spp.) are valued for their health-promoting potential. However, their juices often exhibit excessive acidity and unbalanced flavor, particularly after fermentation, which limits consumer acceptance and product development. This study aimed to improve both sensory quality and functional properties of red and black goji berry juices through probiotic fermentation using a low-acid-producing strain. Four probiotic strains were screened for their fermentation performance in red and black goji berry juices. Among them, Limosilactobacillus fermentum WXZ2-1 exhibited the most balanced fermentation profile; its moderate acid accumulation effectively preserved the sugar-acid balance, leading to a 29.12% increase in overall sensory acceptability scores compared to the average of other strains. L. fermentum WXZ2-1 exhibited superior growth performance in black goji juice, reaching 1.2 × 10 8 CFU/mL at 18 h, and produced a significantly higher sugar-to-acid ratio than other strains (SAR 27.25 in red and 23.33 in black at 24 h). Moreover, fermentation with L. fermentum WXZ2-1 significantly increased the antioxidant activity (DPPH radical scavenging capacity) and total phenolic content by 16.16% and 56.01%, respectively; in red goji berry juice, and by 15.57% and 77.63%, respectively, in black goji berry juice. Metabolomic and volatile analyses revealed enhanced sugar metabolism, moderated acid formation, and enriched aromatic profiles, particularly in black goji juice. Overall, L. fermentum WXZ2-1 is a promising starter for producing fermented goji beverages with improved sensory quality and functional benefits.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Jing Tang,Guangping Qin,Qiuhui Wen,Wenhao Li,Liuci Huang,Jian Liang,Zhijiao Tang,Zhengyi Chen,Yumin Wang
The development of a simple-yet-practical and accurate sensing strategy for rapid detection of Alzheimer's disease (AD)-related miRNA in biofluids holds great promise for early diagnosis and monitoring of AD. Herein, a colorimetric/portable Raman dual-mode nanoprobe was proposed for quantification of AD-related miRNA-574-5p in biological fluids. The MOF-818@Au NPs@ssDNA nanoprobe was prepared by step-by-step loading of gold nanoparticles (Au NPs) and ssDNA onto the surface of MOF-818. Strikingly, the adsorbed ssDNA was capable of greatly improving the intrinsic peroxidase-like activity of MOF-818@Au NPs. In the presence of miRNA-574-5p, the ssDNA onto MOF-818@Au NPs will specifically hybridized with miRNA-574-5p, resulting in the desorption of ssDNA from MOF-818@Au NPs and the decrease of nanoenzyme activity for H 2 O 2 -mediated TMB oxidation. Based on this feature, visualization and quantitative analysis of miRNA-574-5p can be successfully realized by observing and measuring changes in output colorimetric/Raman signal from blue TMB oxide (oxTMB) with portable Raman spectrometer. Experimental results demonstrated that the colorimetric/portable Raman dual-mode nanoprobe can be utilized for fast, sensitive and accurate detection of AD-related miRNA-574-5p in complex human serum samples, providing a universal, valuable and practical tool for AD biomarkers detection and AD early diagnosis.
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Bo Li,Lin Zhang,Ding-Tao Wu,Qin Yuan,Shengpeng Wang,Mao-Ling Tan,Mei-Lin Feng,Yi-Chen Hu,Liang Zou,Zi-Peng Wang,Ya-Nan Cao,Jing-Wei Huang
Tartary buckwheat sprout pectic polysaccharides (TP) have demonstrated notable immunostimulatory activity in vitro. Nevertheless, the in vivo function and how structural features dictate its immunostimulatory activity through gut microbiota modulation are still unclear. To fill this gap in our understanding, we compared in vivo immunostimulatory effects of TP and its derivatives featuring key structural modifications: a lower esterification degree (TP-LDE: 4.72% vs. TP: 28.04%) or a lower molecular mass (TP-LMW: 0.957 × 10 4 Da vs. TP: 8.191 × 10 4 Da). Results demonstrated that TP and its derivatives (TP-LDE, TP-LMW) counteracted cyclophosphamide (CTX)-induced immunosuppression by restoring gut microbiota balance, short-chain fatty acid (SCFA) levels, intestinal barrier function, and systemic immunity. Notably, the derivatives, particularly TP-LMW, exhibited superior immunostimulatory activity to the native TP in enhancing serum cytokines (TNF-α and IFN-γ) and serum immunoglobulin IgA, promoting SCFA (propionic acid and butyric acid) production, and maintaining intestinal barrier function (enhanced expression of ZO-1 and occludin), confirming that reducing either the molecular mass or the esterification degree enhances TP's immunostimulatory potency. Furthermore, all treatments reversed CTX-induced dysbiosis by increasing Muribaculaceae while suppressing dysbiosis-linked genera (e.g., Lachnospiraceae_NK4A136_group , Colidextribacter , and Oscillibacter ). In contrast, only TP-LDE uniquely and significantly enriched the beneficial genera Bacteroides , Prevotellaceae_UCG_001 , and Parabacteroides . Taken together, this work elucidates how structural features govern the immunostimulatory activity of TP, which provides a foundation for developing TP and its derivatives into functional foods for improving and maintaining host immunity.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 4
·233
·
IF 5.4
Gao Xiang,Hou Yujiao,Lin Fang,Wen Limei,Guo Yali,Kong Weijun,Hu Junping,Yang Jianhua
An electrochemical (EC) molecularly imprinted polymer (MIP) sensor for therapeutic drug monitoring of olanzapine (OLZ) has been developed. A “rime-like” gold-platinum nanostructured film (Au-Pt NF) was fabricated on a screen-printed carbon electrode (SPCE) via an one-step hydrogen bubble dynamic template (HBDT) method, followed by electropolymerization of a polyaniline-based MIP. The Au-Pt NF/SPCE served as an ideal three-dimensional conductive substrate with a large active area and enhanced electron-transfer capability, endowing the specific MIP sensor with high sensitivity. Using [Fe(CN) 6 ] 3−/4− as a redox probe, the sensor quantified OLZ by the decrease in differential pulse voltammetry current caused by target binding. It exhibited two linear ranges (0.005–0.4 µM and 0.4–10 µM) with a detection limit of 0.94 nM, and demonstrated high selectivity, reproducibility, and stability. The sensor achieved recoveries of 96.7–102.7% for OLZ in tablets, urine, and whole blood. This work provides a reliable, all-EC sensing platform with strong potential for rapid OLZ monitoring in clinical settings.
Section
Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2026
·
IF 4.4
Cheng Li-Qian,Gong Yi,Chen Kai,Hao Xuxia,Jiang Min,Dai Yu,Xie Xinyuan
LiMn 0.8 Fe 0.2 PO 4 (LMFP) cathode has emerged as a promising candidate for high-energy–density lithium-ion batteries due to its cost-effectiveness, high voltage plateau and intrinsic safety. However, drawbacks such as low electrical conductivity, Jahn–Teller effect and Mn 2+ dissolution severely limit its application potential. Herein, we propose a strategy that couples B-F co-doping with HAP@PP separator modification to simultaneously boost the conductivity of LMFP and mitigate Mn 2+ leaching using a facile solid-phase method. The optimized LiMn 0.8 Fe 0.2 P 0.99 B 0.01 O 3.97 F 0.03 (LMFP-BF1) exhibits superior rate capability and excellent cycle stability, which delivers a discharge capacity of 137.4 mAh g −1 and retains 92.6% of that capacity at 1C after 300 cycles. Moreover, HAP@PP separator exhibits an excellent manganese capture effect, which reduces the precipitation of manganese content from 134.647 to 14.056 mg kg −1 . The LMFP-BF1|HAP@PP cell remains a discharge capacity of 140.6 mAh g −1 at 0.5–45 ℃ after 100 cycles, with a capacity retention rate of 88.31%. This study offers insights into integrating ion doping and separator modification for phosphate-based high-energy–density lithium-ion batteries. Graphical abstract B-F co-doping and modified HAP@PP separator can simultaneously boost the conductivity of LMFP and mitigate Mn 2+ leaching, delivering superior cycling stability.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2026
·
IF 6.8
Zhuoshuo Zhou,Weirong Sun,Liye Yang,Yue Li,Hongpeng Guo,Ximing Cui,Yanli Zhao,Jinlian Li,Gang Wang,Dongmei Wu
Ethnopharmacological relevance Physalis alkekengi L. ( P. alkekengi ) is widely used in traditional medicine and functional foods for its various medicinal properties. However, its toxicological profile has not been comprehensively assessed. Aim of the study This study aimed to characterize the developmental toxicity and cardiotoxicity of P. alkekengi using a Danio rerio ( D. rerio ) embryo model and to identify key toxic constituents to support safety optimization. Materials and methods D. rerio embryos were exposed to the fruit and calyx extracts of P. alkekengi as well as membrane-fractionated preparations to assess toxic phenotypes. Chemical profiling was performed using ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-Exactive Orbitrap-MS), and mechanistic insights were explored using apoptosis-related assays and network toxicology analysis. Results The fruit extract of P. alkekengi exhibited stronger developmental toxicity and cardiotoxicity effects compared to the calyx extract. The 5–10 kDa non-polysaccharide fraction of the fruit was identified as the primary toxic source, with Glycidyl stearate (GS) being the key cardiotoxic component in this fraction. Removal of the most toxic 5–10 kDa fraction during the extraction process significantly reduced the toxicity of the fruit extract. Conclusion The toxicity of P. alkekengi fruit extract is concentrated in the non-polysaccharide 5–10 kDa fraction, with GS being the main contributor to the toxicity in this fraction. Membrane-based separation technology to remove this fraction can enhance the safety of the extract. This study provides a framework for the safety evaluation and optimization of traditional medicinal plants.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Nguyen Ngoc Huyen,Ngo Xuan Dinh,Vu Thi Huong Mai,Phung Thi Lan Huong,Nguyen Duc Toan,Nguyen Anh Son,De Pham-Cong,Van Tan Tran,Hoang Van Tuan,Nguyen Quang Hoa,Pham Duc Thang,Anh-Tuan Le
This study reports the carbendazim (CBZ) detecting capability of spinel cobaltite MnCo 2 O 4 (MCO) synthesized via a microwave-assisted hydrothermal approach. The MCO-modified screen-printed electrode (MCO/SPE) exhibited excellent electrocatalytic activity toward CBZ oxidation. As a result, the proposed sensor enables sensitive CBZ detection over two distinct linear ranges (0.05–5 μM and 25–500 μM), with a low detection limit of 0.02 μM and a high sensitivity of 6.46 μA μM −1 cm −2 . The enhanced sensing performance is attributed to the synergistic redox activity of Co 2+ /Co 3+ and Mn 2+ /Mn 3+ couples, which enhance both electrical conductivity and catalytic activity, together with the nanorod morphology that promotes efficient diffusion of CBZ molecules and electrolyte species. The applicability of the MCO/SPE was further validated through effective CBZ detection in fruit and vegetable samples as compared with the liquid chromatography–tandem mass spectrometry (LC-MS/MS) method, showing strong potential for food safety monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2026
·
IF 5.8
Xinxin Li,Yueming Yu,Siyu Li,Xueyang Lin,Chen Yang,Yufeng Yang,Shengran Wang,Mengwei Zhou,Zhenghao Bao,Xin Sui,Wenya Feng,Jun Yang,Daiying Zuo,Yuan Luo,Yongan Wang,Xianli Du
Acetylcholinesterase (AChE) is a crucial hydrolytic enzyme in the central nervous system, responsible for the rapid degradation of the neurotransmitter acetylcholine (ACh) in the synaptic cleft, thereby maintaining the balance between neuronal excitation and inhibition. AChE is not only the primary target of neurotoxic agents and organophosphorus pesticides but its aberrant activity is also closely associated with various neurodegenerative diseases such as Alzheimer’s disease (AD) and myasthenia gravis. The efficient and rapid discovery and screening of AChE inhibitors hold urgent and significant value for chemical toxin detection, toxicological research, and drug development for neurodegenerative diseases. Addressing the limitations of existing methods, such as low biocompatibility, low detection throughput, relative operational complexity, and high cost, this study innovatively utilizes a genetically encoded biosensor to construct a stable cell line co-expressing the ACh probe and AChE, establishing a novel high-throughput screening method for AChE inhibitors. The results demonstrate that this method achieved to detect AChE inhibitors at micromole level. This method eliminates the need for purified enzymes and toxic chemical reagents (e.g., DTNB in Ellman’s assay), significantly reduces cost (by approximately two orders of magnitude), and offers a simplified, rapid, and high-throughput compatible workflow for applications in neurotoxin detection and neurotherapeutic drug discovery.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR DIVERSITY
·
2026
·
IF 4.3
Deng Yasi,Liang Ling,Lin Haokai,Shen Xinyang,Zheng Hao,Deng Ying,Tian Xing,Huang Juan,Zhang Ye,Li Bin,Yu Huanghe,Wang Wei
Eucommiae Folium (EF) is a dual-purpose substance utilized in both medicine and nutrition, commonly incorporated into porridge and tea. Traditionally, it is recognized for its ability to tonify the kidney and liver, strengthen muscles and bones, lower blood pressure, and exhibit anti-aging effects. This study aims to investigate the primary components and mechanisms underlying the anti-rheumatoid arthritis (RA) properties of aqueous extract of EF. To identify the material basis of aqueous extract of EF, we employed liquid chromatography-mass spectrometry (LC-Orbitrap-MS) for component analysis using database software. Subsequently, a network approach was applied to pinpoint key anti-RA targets and pathways in order to validate its potential as an effective anti-RA agent. An adjuvant-induced arthritis (AIA) rat model was established, with oral administration of aqueous extract of EF over 30 consecutive days to evaluate its therapeutic effects. The results indicated that LC-Orbitrap-MS identified 29 principal compounds, including geniposidic acid, asperuloside, chlorogenic acid, kaempferol, aucubin, and trifolin. Pharmacological network analysis suggested that these components may exert their therapeutic effects on RA through modulation of NF-κB and PI3K-AKT signaling pathways. Furthermore, oral administration significantly reduced paw swelling in AIA rats while histological analysis demonstrated suppression of inflammatory responses in paw tissues by inhibiting NF-κB and PI3K-AKT pathways.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2026
·
IF 5.4
Chuanju Wang,Qiang Peng,Qianhua Zhang,Shihua Han,Lingyong Zeng,Jiarong Su,Guixin Liang,Zihao Chen,Juan Guo,Yongguang Bi,YuShi Gong,Shaofan Tan,Huaicheng Qin
Pithecellobium clypearia Benth polyphenols (PCBPL) possess potent antioxidant and anti-inflammatory properties, making them promising agents for ulcerative colitis (UC) therapy. However, their clinical translation is hindered by poor water solubility, low stability, and limited bioavailability. To overcome these limitations, Pithecellobium clypearia Benth polyphenols liposomes (PCBPL-Lips) were prepared via thin-film hydration. The resulting PCBPL-Lips exhibited a high encapsulation efficiency of 89.31 ± 1.09%, an average particle size of 124.51 ± 7.61 nm, and a zeta potential of −31.62 ± 2.37 mV. Transmission electron microscopy confirmed their uniform particle distribution, spherical morphology, and smooth surfaces. Stability evaluations showed that PCBPL-Lips remained stable under various pH, temperature, and ionic strength conditions. In vitro digestion studies revealed a sustained-release profile, suggesting potential for prolonged therapeutic action. Biocompatibility assessments showed a hemolysis rate below 5% and cell viability above 80%, indicating excellent safety. In vitro activity assays demonstrated that liposomal encapsulation preserved the antioxidant activity of PCBPL and significantly enhanced its anti-inflammatory efficacy. In a murine model of UC, PCBPL-Lips alleviated clinical symptoms, reduced body weight loss, increased colon length, decreased spleen index and tumor necrosis factor-alpha (TNF-α) levels, and improved colonic tissue architecture. This study demonstrates that liposomal encapsulation markedly enhances the stability, bioavailability, and therapeutic efficacy of PCBPL, providing a promising strategy for its use in the treatment of inflammatory bowel diseases.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Han Zhiqiang,Chen Qiao,Duan Xinmei,He Zhigui,Mou Nianlian,Cao Yu,Ou Yangsipei,He Houhua,Zhang Can,Yang Xu,Qu Kai,Zhang Kun,Malashicheva Anna,Zhong Yuan,Li Chuanwei,Fu Li,Wang Guixue,Wu Wei
Atherosclerotic plaques are characterized by oxidative stress and inflammatory responses, which amplify each other in a vicious cycle, leading to progressive plaque destabilization and ultimately serious cardiovascular events. Therefore, the development of nanoformulations with synergistically enhanced anti-inflammatory and antioxidant activities and effective aggregation in plaques is ideal. In this study, we prepared a gallic acid (GA)-cerium metal polyphenol nanoconjugate (GA-Ce, GC) through cerium ions-mediated oxidative coupling of GA, followed by functionalization of its surface with a phosphatidylserine (PS)-binding peptide (GC-PS, GCP), and subsequent encapsulation with a P-selectin glycoprotein ligand-1 (PSGL-1)-overexpressing endothelial cell membrane (PEM) to harvest a biomimetic PEM@GCP nanozyme. The in vitro results showed that PEM@GCP could effectively scavenge free radicals, promote macrophage differentiation to the M2 phenotype, reduce the content of proinflammatory cytokines, inhibit lipid peroxidation, and inhibit apoptosis with cytoprotective functions. In the atherosclerotic mouse model, compared with the control, PSGL-1-based PEM@GCP resulted in a 4.04-fold greater accumulation in plaque lesions. Through its synergistic anti-inflammatory and antioxidant activities, it effectively blocks the vicious cycle of inflammation-oxidative stress, thereby significantly ameliorating the pathological microenvironment and leading to a substantial 71.3% reduction in the plaque area. These studies elucidate the therapeutic potential of a biomimetic metal-polyphenol nanozyme in the treatment of atherosclerosis, demonstrating its potential as a promising candidate for clinical translation. Graphical
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Qingfei He,Lingjun Cheng,Yuanyuan Yang,Zhiyan Zou,Canping Su,Xuan Shi,Jiaming Chen,Weirong Huang,Zhenyu Lin,Guolin Hong
Gold nanoclusters (AuNCs) are emerging electrochemiluminescence (ECL) emitters with unique luminescence and high stability. However, conventional AuNC-based ECL systems, which rely on single-signal regulation, often exhibit weak signal responses and limited stability. To address the challenge of detecting extremely low concentrations of the Alzheimer’s disease (AD) blood biomarker Aβ40, this study presents a novel ECL-based biosensing platform that leverages dual-signal regulation of AuNCs via glutathione (GSH) and H2O2 serving as ECL quenchers. Based on the peroxidase-like activity of the Aβ40–hemin complex, this system restores the ECL signal by catalyzing the consumption reaction between GSH and H2O2. Using this mechanism, a dual-regulation ECL platform was developed, demonstrating a linear detection range of 0.001–100 ng/mL for Aβ40 with a detection limit of 0.18 pg/mL (S/N = 3). This approach not only offers high sensitivity and specificity but also reduces the complexity of surface modification steps. Overall, this study advances ECL technology through dual regulation and provides a sensitive, practical, efficient, and reliable analytical method for early AD screening.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）