MOLECULES
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2023
·Vol. 28
·期 13
·5172
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IF 4.6
Yue Sun,Jia-Xin Feng,Zhong-Bao Wei,Hui Sun,Li Li,Jun-Yi Zhu,Guang-Qing Xia,Hao Zang
Euphorbia fischerianahas a long-standing history of use in traditional medicine for the treatment of tuberculosis diseases. However, the plant’s therapeutic potential extends beyond this specific ailment. The present study aimed to investigate the antioxidant properties ofEuphorbia fischerianaand lay the groundwork for further research on its potential therapeutic applications. Phytochemical tests were performed on the plant, and 11 types of phytochemicals were identified. Ultraviolet–visible spectrophotometry was used to evaluate the active components and antioxidant properties of eight different solvent extracts, ultimately selecting acetone extract for further research. UHPLC-ESI-Q-TOF-MS identified 43 compounds in the acetone extract, and chemical calculations were used to isolate those with high content and antioxidant activity. Three stability experiments confirmed the extract’s stability, while cell viability and oral acute toxicity studies demonstrated its relatively low toxicity. In rats, the acetone extract showed significant protective effects against D-galactosamine-induced liver damage through histopathological examination and biochemical analysis. These results suggest thatEuphorbia fischeriana’s acetone extract has potential in treating diseases related to oxidative imbalances. Therefore, this study highlights the plant’s potential therapeutic applications while providing insight into its antioxidant properties.Keywords:Euphorbia fischeriana;phytochemical analysis;antioxidant activity;theoretical calculation
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Journal of Orthopaedic Surgery and Research
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2023
·Vol. 18
·期 1
·1-15
·
IF 2.6
Sun Kai,Xue Yuman,Zhang Xin,Li Xiaodong,Zhao Jun,Xu Xilin,Zhang Xiaofeng,Yang Fubiao
Background The impaired blood supply to the bones is an important pathological feature of steroid-induced osteonecrosis of the femoral head (SIONFH). Danshen is a Chinese herb that shows therapeutic effects on SIONFH, but the effects of one of its major bioactive constituents, Tanshinone I (TsI), on SIONFH remain unknown. Here, we evaluated the effects of TsI on SIONFH, particularly focusing on its effects on angiogenesis, in in vivo and in vitro research. Methods SIONFH was induced in Sprague–Dawley rats by an intramuscular injection of methylprednisolone (40 mg/kg) in combination with an intraperitoneal injection of lipopolysaccharide (20 μg/kg). Morphological alterations of the femoral head were observed by dual-energy X-ray absorptiometry and HE staining. Western blot, qRT-PCR, and immunohistochemical/immunofluorescence staining were used to determine gene expression. Results TsI (10 mg/kg) alleviated bone loss and rescued the expression of angiogenesis-related molecules (CD31, VWF, VEGF, and VEGFR2) in the femoral heads of SIONFH rats. Notably, TsI rescued the down-regulated expression of SRY-box transcription factor 11 (SOX11) in CD31 + endothelial cells in the femoral heads of SIONFH rats. In vitro studies showed that TsI preserved the dexamethasone-harmed angiogenic property (migration and tube formation) of human umbilical vein cells (EA.hy926), suppressed dexamethasone-induced cell apoptosis, reduced pro-apoptotic proteins (cytosolic cytochrome C, Bax, and caspase 3/9) and increased anti-apoptotic protein Bcl-2, whereas silencing of SOX11 reversed these beneficial effects. Conclusions This study demonstrates that TsI alleviates SIONFH and promotes angiogenesis by regulating SOX11 expression. Our work would provide new evidence for the application of TsI to treat SIONFH. Graphical Abstract
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ACTA PHARMACOLOGICA SINICA
·
2023
·
IF 8.2
Wang Jie,Bai Mei,Zhang Cui,An Ning,Wan Li,Wang Xiao-ning,Du Rong-hui,Shen Yan,Yuan Zhi-yao,Wu Xu-dong,Wu Xue-feng,Xu Qiang
Intestinal fibrosis is a common complication of inflammatory bowel disease. There is still a lack of effective drugs for the prevention or treatment of intestinal fibrosis. Heat shock protein 47 (HSP47) plays a key role in the development of intestinal fibrosis. In this study we investigated the therapeutic potential and underlying mechanisms of fraxinellone, a degraded limonoid isolated from the root bark of Dictamnus dasycarpus , in the treatment of intestinal fibrosis. Intestinal fibrosis was induced in mice by dextran sodium sulfate (DSS) treatment. DDS-treated mice were administered fraxinellone (7.5, 15, 30 mg·kg −1 ·d −1 , i.g .) for 45 days. We showed that fraxinellone administration dose-dependently alleviated DSS-induced intestinal impairments, and reduced the production of intestinal fibrosis biomarkers such as α-smooth muscle actin (SMA), collagen I, hydroxyproline, fibronectin and laminin, and cytokines such as TGF-β, TNF-α and IL-β. We then established in vitro intestinal fibrosis cell models in SW480 and HT-29 cells, and demonstrated that treatment with fraxinellone (3, 10, 30 μM) significantly relieved TGF-β-induced fibrosis responses by inhibiting the TGF-β/Smad2/3 signaling pathway. Molecular docking suggested that the fraxinellone might disrupt the interaction between HSP47 and collagen, which was confirmed by coimmunoprecipitation experiments. SPR analysis showed that fraxinellone had a high affinity for HSP47 with a K d value of 3.542 × 10 −5 M. This study provides a new example of HSP47-collagen intervention by a natural compound and has important implications for the clinical treatment of inflammation-induced issue fibrosis.
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PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY
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2023
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IF 5.6
Jiao-Zhao Yan,Guang-Xiang Li,Si-Rui Sun,Lin-Yu Cui,Yong-Yu Yin,Yun-Feng Li
Although clinical antidepressants have varied mechanisms of action, it remains unclear whether they may have a common mechanism underlying their antidepressant effects. We investigated the behavioral effects of five different antidepressants (differing in target, chemical structure, and rate of onset) and their effects on the firing activities of glutamatergic pyramidal neurons in the medial prefrontal cortex (mPFC) using the forced swimming test (FST) and electrophysiological techniques (in vivo). We employed fiber photometry recordings to validate the effects of antidepressants on the firing activity of pyramidal neurons. Additionally, multichannel electrophysiological recordings were conducted in mice exhibiting depressive-like behaviors induced by chronic restraint stress (CRS) to investigate whether antidepressants exert similar effects on pyramidal neurons in depressed mice. Behavioral tests were utilized for evaluating the depression model. We found that fluoxetine , duloxetine , vilazodone , YL-0919, and ketamine all increase the firing activities of glutamatergic pyramidal neurons (at least 57%) while exerting their initial onset of antidepressant effects. Fiber photometry revealed an increase in the calcium activity of pyramidal neurons in the mPFC at the onset of antidepressant effects. Furthermore, a significant reduction was observed in the firing activity of pyramidal neurons in the mPFC of CRS-exposed mice, which was reversed by antidepressants. Taken together, our findings suggested that five pharmacologically distinct classes of antidepressants share the common ability to increase the firing activity of pyramidal neurons, just different time, which might be a rate-limiting step in antidepressants onset. The study contributes to the body of knowledge of the mechanisms underlying antidepressant effects and paves the way for developing rapid-acting antidepressants.
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International Journal of Environmental Research and Public Health
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2022
·Vol. 19
·期 19
·12547
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IF 4.614
Nan Wang,Zhengwu Cui,Yang Wang,Jingjing Zhang
In this study, eleven organochlorine pesticides (OCPs) in fresh vegetables in the Changchun suburb were investigated, and their potential health risks were evaluated. The average concentrations of OCPs in edible parts of vegetables were found in the following descending order: Σhexachlorocyclohexanes (ΣHCHs) (6.60 µg·kg−1) > Σdichlorodiphenyltrichloroethanes (ΣDDTs) (5.82 µg·kg−1) > ΣChlordanes (2.37 µg·kg−1) > heptachlor (0.29 µg·kg−1). Moreover, OCPs in different types of vegetables exceeded the maximum residue limits (MRLs), and the exceeding rates in various vegetables decreased in the following order: leafy vegetables (19.12%) > root vegetables (18.75%) > fruit vegetables (3.85%). The proportions of OCPs exceeding MRL in different vegetables were found in the following descending order: Welsh onion (22.50%) > radish (18.75%) > Chinese cabbage (14.29%) > pepper (6.90%) > cucumber (3.23%) > eggplant (2.94%) > tomato (2.78%). The sources’ identification results showed that DDTs in vegetables came mainly from newly imported technical DDTs and dicofol, while HCHs originated mainly from lindane. For both adults and children, the average target hazard quotients (avg. THQ) were all less than 1, and the average hazard index (avg. HI) values were 0.043 and 0.036, respectively. There were no significant health risks associated with OCP exposure for the inhabitants of the study area.Keywords:suburban vegetables;organochlorine pesticides;maximum residue limit (MRL);sources identification;target hazard quotient (THQ)
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BIOMACROMOLECULES
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2022
·
IF 6.978
Lu Wan,Liangliang Lu,Tangsong Zhu,Zhichang Liu,Ruichun Du,Qiong Luo,Qiang Xu,Qiuhong Zhang,Xudong Jia
As a biodegradable elastomer, poly(1,8-octanediol-co-citrate) (POC) has been widely applied in tissue engineering and implantable electronics. However, the unclear degradation mechanism has posed a great challenge for the better application and development of POC. To reveal the degradation mechanism, here, we present a systematic investigation into in vivo and in vitro degradation behaviors of POC. Initially, critical factors, including chemical structures, hydrophilic and water-absorbency characteristics, and degradation reaction of POC, are investigated. Then, various degradation-induced changes during in vitro degradation of POC-x (POC with different cross-linking densities) are monitored and discussed. The results show that (1) cross-linking densities exponentially drop with degradation time; (2) mass loss and PBS-absorption ratio grow nonlinearly; (3) the morphology on the cross-section changes from flat to rough at a microscopic level; (4) the cubic samples keep swelling until they collapse into fragments from a macro view; and (5) the mechanical properties experience a sharp drop at the beginning of degradation. Finally, the in vivo degradation behaviors of POC-x are investigated, and the results are similar to those in vitro. The comprehensive assessment suggests that the in vitro and in vivo degradation of POC occurs primarily through bulk erosion. Inflammation responses triggered by the degradation of POC-x are comparable to poly(lactic acid), or even less obvious. In addition, the mechanical evaluation of POC in the simulated application environment is first proposed and conducted in this work for a more appropriate application. The degradation mechanism of POC revealed will greatly promote the further development and application of POC-based materials in the biomedical field.
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CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Yizhen Cheng,Binyuan Wang,Pengwei Yan,Jimin Shen,Jing Kang,Shengxin Zhao,Xinwei Zhu,Linlu Shen,Shuyu Wang,Yang Shen,Zhonglin Chen
Adsorption is the first step of the interface mechanism, but the adsorption behaviors of ozone (O 3 ) and pollutants on the catalyst during catalytic ozonation have always been overlooked in previous works. In this study, a promising strategy for the in-situ decomposition of O 3 to trigger surface reactive oxygen species (ROS) by nitrogen (N)-doped biochar was proposed, which greatly improved the efficiency of O 3 utilization. Specifically, N -doped biochar (NBC700) with a high defect level ( I D /I G = 1.165) was achieved by a one-pot method. It showed good adsorption on O 3 and atrazine (ATZ), which promoted the in-situ formation of surface ROS, as well as resists the interferences of multiple coexisting anions (NO 3 − , Cl − , PO 4 3− , SO 4 2− and HCO 3 − ) on ATZ removal. In-situ Raman spectra revealed the interface catalytic mechanism of O 3 decomposition into adsorbed peroxide species (*O 2 ) and adsorbed atomic oxygen (*O). Additionally, OH was the dominant ROS and surface-O 3 further strengthened direct ozonation via intramolecular electron transfer. In this process, sp 2 -hybridized system with delocalized π electrons, electron-rich oxygen-containing functional groups, and conjugated heteroatoms were identified as the active sites, but defective sites with free electrons played the most important part according to the lowest adsorption energy (−13.12 eV) calculated by density functional theory (DFT). The degradation of ATZ included dechlorination and non-dechlorination pathways, which made the acute and chronic toxicity of most intermediate products both decrease to not be harmful to fish and green algae. This work provides a new perspective on the interface mechanism in catalytic ozonation for ATZ removal.
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ANALYTICA CHIMICA ACTA
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2022
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IF 6.911
Feifeng Yang,Yue Zhu,Congyu Zhang,Ziyan Yang,Jia Yuan,Qing Zhu,Shushu Ding
As the most aggressive reactive oxygen species (ROS), hydroxyl radical (•OH) can directly modulate the biological ion channel and interfere with the progression of diseases. Inspired by biological •OH-activated ion channel, we reported a novel •OH-regulated glass nanopore functionalized with protoporphyrin Ⅸ (PP) film. This system showed outstanding •OH selective response owing to the ultra-fast reaction between •OH and thiol derivatives. In this case, the PP film is responsible for the changing not only of wettability but also of the inner surface charge. The synergetic effect of the dual transitions can regulate the ion transportation within the nanochannels and enabled tremendous enhancement of responsive efficiency. The detection limit could be achieved down to 1.58 nM. Taking advantage of the excellent analytical performance and mechanical qualities of this glass nanopore, the changes of •OH in single living cells were in situ monitored. Together, this study is beneficial for exploring the role of •OH in pathological events and shows promising potential for biomedical research.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2022
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IF 9.965
Qinghai Ma,Fang Cui,Jiajia Zhang,Tieyu Cui
Metal oxides are promising electrode candidates for supercapacitor due to their high theoretical capacitance, good reversibility, and low cost. However, they show inferior specific capacitance and power density because of their sluggish ion diffusion kinetics and intrinsically poor electrical conductivity within the solid phase. Herein, heterostructured ZnCo 2 O 4 /ZnO nanobelts are successfully prepared by using self-assembled Zn/Co-based nanosized coordination polymers as the precursors. The resulted nanobelts are composed of uniformly distributed ZnCo 2 O 4 and ZnO nanocrystals, which spontaneously develop built–in electric fields in the nanobelts, and thus improve the conductivity and accelerate charge transport. The as-obtained ZnCo 2 O 4 /ZnO nanobelts display a high specific capacitance of 481.0 F g −1 at 1 A/g. The asymmetric supercapacitor , with a ZnCo 2 O 4 /ZnO positive electrode and an activated carbon negative electrode, deliver an energy of 23.77 Wh kg −1 at the power density of 399.98 W kg −1 and excellent prolonged cycle life. The excellent electrochemical performance benefits from both the special structure and built–in field at the heterostructure interface, which could significantly reduce the ion diffusion resistance and thus promote charge transport. This strategy may blaze a trail for engineering efficient electrode based on earth-abundant materials.
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AQUATIC TOXICOLOGY
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2022
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IF 5.202
Jia Hu,Jingjing Tian,Tongkuo Yuan,Qingqing Yin,Jian Yin
Despite the increasing evidences for adenosine triphosphate-binding cassette (ABC transporters)-mediated efflux of nanoparticles, the universality of these phenomena and the determining factors for the process remained to be clarified. This paper aimed to systemically investigate the role of nanoparticle size in the interactions between adenosine triphosphate-binding cassette (ABC transporters) and gold nanoparticles (AuNPs, 3 nm, 19 nm, and 84 nm, named as Au-3, Au-19, and Au-84) in zebrafish embryos. The results showed that all the three AuNPs induced significant toxicity as reflected by delayed hatching of embryos, decreased glutathione (GSH) contents, and increased reactive oxygen species (ROS) levels. Under the hindrance of embryo chorions, smaller AuNPs could more easily accumulate in the embryos, causing higher toxicity. Addition of transporter inhibitors enhanced the accumulation and toxicity of Au-3 and Au-19, and these nanoparticles induced the expressions of abcc2 and abcb4 , indicating a fact that Au-3 and Au-19 were the potential substrates of ABC transporters, but these phenomena were barely found for Au-84. On the contrary, Au-84 suppressed the gene expressions of various ABC transporters like abcc1, abcg5, and abcg8 . With specific suppressors, transcription factors like nuclear factor-erythroid 2-related factor-2 (Nrf2) and pregnane X receptor (Pxr) were found to be important in the induction of ABC transporters by AuNPs. After all, these results revealed a vital role of nanoparticle sizes in the interactions between ABC transporters and AuNPs in zebrafish embryos, and the critical size could be around 19 nm. Such information would be beneficial in assessing the environmental risk of nanoparticles, as well as their interactions with other chemical toxicants.
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Scientific Reports
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2022
·Vol. 12
·期 1
·1-11
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IF 4.996
Li Shuangde,Wang Li,Guan Shanyue,Zhou Shuyun,Chen Yunfa
Cooking oil fumes (COFs) represent a major indoor environmental pollutant and exhibit potent mutagenic or carcinogenic health effects caused by containing various heterocyclic aromatic amines (HAAs) and long-chain aldehydes. Despite some evaluation of the cumulative exposure of COFs to cancer cells under high concentration were evaluated, their biological adverse effects with low-dose exposure to healthy cells had been inadequately investigated. Herein, we firstly scrutinized the three selected typically toxic compounds of heterocyclic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 3,8-dimethylammidazo[4,5-f]quinoxalin-2-amine (MeIQx) and trans, trans-2,4-decadienal (TDA)) emitted from COFs. In vitro studies revealed that the PhIP, MeIQx and TDA aerosol particles were negligible toxicity to cancer cells (A549 and HepG-2) but strong cytotoxicity to normal healthy cells (HelF and L02) under 0.5–4 μg/mL low dose exposure based on the reactive oxygen species (ROS) mechanism. In vivo studies demonstrated that PhIP caused significant lung and liver damage after exposure to PhIP for 30 days with mice. These results indicated the direct proof of healthy cell damage even at low-dose exposure to HAAs and aldehydes.
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Materials Chemistry Frontiers
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2022
·Vol. 6
·期 21
·3205-3212
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IF 8.683
Jiangting Zhao,Zhuo Xiong,Junyi Wang,Yaqin Qiu,Yongchun Zhao,Pengfei Liu,Junying Zhang
In this study, a 1D/2D ZnO nanofiber/graphdiyne oxide heterojunction was designed for photocatalytic CO2 reduction, and the difference between graphdiyne and graphdiyne oxide was investigated as co-catalysts. The ZGDO heterostructure has strong reactant adsorption capacity due to the enlarged specific surface area and abundant hydrophilic oxygen-containing functional groups. DFT calculations, EPR, and in situ XPS further verified the S-scheme charge transfer mechanism in ZGDO. In contrast, the abilities of GD to adsorb CO2 and enhance light absorption are not as good as those of GDO, and the band structure of GD is not conducive to the formation of a favorable electric field drive at the interface with ZnO. The CO yield of ZGDO with only 3% GDO was up to 34.9 μmol h−1 g−1 in the gas–phase photocatalytic reduction of CO2, which was 4.4, 24, and 1.5 times that of ZnO, GDO, and ZnO/GD, respectively. This work opens new possibilities for the application of new non-metallic photocatalysts in the photoreduction of CO2.
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