NANOTECHNOLOGY
·
2024
·Vol. 36
·期 6
·065101
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IF 2.9
Xu Chen,Jian Yue,Xiongjun Xu,Jiajun Chen,Xuechan Huang,Yukai Huang,Yang Yang,Feng Li,Tianwang Li
Selenium-based nanoparticles exhibit antiviral activity by directly modulating immune function. Despite recent promising developments in utilizing selenium nanoparticles (Se NPs) against viral infections, the impact of surface ligand charge on the conformation and interaction with viral proteins, as well as the effectiveness of Se NPs in anti-Herpes simplex virus 1 (HSV-1) infection remains unexplored. In this study, three types of selenium nanoparticles (CTAB-Se, PVP-Se, SDS-Se) with distinct surface charges were synthesized by modifying the surface ligands. We found that apart from differences in surface charge, the size, morphology, and crystal structure of the three types of Se NPs were similar. Notably, although the lipophilicity and cellular uptake of SDS-Se with a negative charge were lower compared to positively charged CTAB-Se and neutrally charged PVP-Se, SDS-Se exhibited the strongest protein binding force during interaction with HSV-1. Consequently, SDS-Se demonstrated the most potent anti-HSV-1 activity and safeguarded normal cells from damage. The mechanistic investigation further revealed that SDS-Se NPs effectively inhibited the proliferation and assembly of HSV-1 by powerfully suppressing the key genes and proteins of HSV-1 at various stages of viral development. Hence, this study highlights the significant role of surface ligand engineering in the antiviral activity of Se NPs, presenting a viable approach for synthesizing Se NPs with tailored antiviral properties by modulating surface charge. This method holds promise for advancing research on the antiviral capabilities of Se NPs.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Yuvaraj Ravikumar,Abdullah Arsalan,Xinrui Tang,Guoyan Zhang,Xianghui Qi
l-Arabinose isomerase (l-AI) catalyzes d-galactose to produce the rare, industrially important sugar d-tagatose. Enzyme stability is vital for its application in industrial processes, and rational design-based protein engineering methods have been employed to improve its stability. This study employed molecular dynamics simulations (MDS)-guided rational redesign of flexible regions to improve the thermostability of mesophilic Bifidobacterium adolescentis l-AI (Ba-l-AI). Comparative MDS (200 ns, 340–360 K) of thermophilic l-AIs (Geobacillus kaustophilus Gk-l-AI and Thermotoga maritima Tm-l-AI) and mesophilic Ba-l-AI identified flexible regions in Ba-l-AI via RMSF and ionic interaction. Five stabilizing mutations (G78C, K46R, N187R, K112R, and N190R) were introduced to provide rigidness. MDS of the mutant revealed reduced RMSF/RMSD, improved compactness, and enhanced conformational stability across temperatures, supported by free-energy landscape analysis resembling thermophilic profiles. Experimentally, the mutant exhibited superior thermal stability (72 °C), broader pH/temperature tolerance, and efficient d-tagatose production. The redesigned Ba-l-AI’s rigid backbone and stability highlight its potential for industrial d-tagatose biosynthesis.
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EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
·
2025
·
IF 1.8
Run-Yang Zhang,Jing-Ru Jia,Peng-Xiao Chen,Wen-Xue Zhu,Hua-Min Liu
Tiger nut ( Cyperus esculentus L.) is a promising oilseed for its high-oleic oil with superior oxidative stability. However, the oxidative stability of crude tiger nut oil varies significantly between extraction batches, a phenomenon hypothesized to correlate with the raw material's moisture content. In this study, tiger nut oil (labeled as TO-4, TO-8, TO-12, TO-16, and TO-20) was obtained by solvent extraction from tiger nuts with varying moisture contents (4%–20%). All oils exhibited no obvious difference in the major triacylglycerol and fatty acids, with low acid values (1.30–1.35 mg/g) and peroxide values (0.14–0.15 mmol/kg). Phospholipids (0.71–8.61 mg/g) and phenolic compounds (24.34–70.83 mg/kg) exhibited significant inter-sample variations, whereas tocopherols, carotenoids, and sterols showed no significant differences. Notably, phospholipids and phenolic compounds demonstrated a significant positive correlation with oxidative stability as measured by induction period (21.9–105.2 h), with TO-12 displaying the longest induction period (105.2 h). Centrifugation of oils confirmed phospholipid and phenolic removal decreased induction periods. Therefore, targeted moisture adjustment for tiger nuts enhanced the oxidative stability of solvent-extracted oil through elevated levels of phospholipids and phenolic compounds in the oil. This work provides a green strategy for improving oil quality of tiger nut. Practical Applications : In this study, how the moisture content of tiger nuts influences the oxidative stability of tiger nut oil was explored, offering guidance for selecting the optimal moisture parameters during oil processing. Employing tiger nuts with 12% moisture content during oil extraction produces crude oil exhibiting optimal oxidative stability. This moisture adjustment strategy not only enhances oil quality through an environmentally benign process but also reduces production and refining expenditures.
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Biosensors-Basel
·
2024
·Vol. 14
·期 3
·121
·
IF 4.9
Chuanxiang Zhang,Song Li,Lingxiao Tang,Shuo Li,Changchun Hu,Dan Zhang,Long Chao,Xueying Liu,Yimin Tan,Yan Deng
Development of an efficient technique for accurate and sensitive dibutyl phthalate (DBP) determination is crucial for food safety and environment protection. An ultrasensitive molecularly imprinted polymers (MIP) voltammetric sensor was herein engineered for the specific determination of DBP using poly-l-lysine/poly(3,4-ethylenedioxythiophene)/porous graphene nanocomposite (PLL/PEDOT−PG) and poly(o-phenylenediamine)-imprinted film as a label-free and sensing platform. Fabrication of PEDOT−PG nanocomposites was achieved through a simple liquid–liquid interfacial polymerization. Subsequently, poly-l-lysine (PLL) functionalization was employed to enhance the dispersibility and stability of the prepared PEDOT−PG, as well as promote its adhesion on the sensor surface. In the presence of DBP, the imprinted poly(o-phenylenediamine) film was formed on the surface of PLL/PEDOT−PG. Investigation of the physical properties and electrochemical behavior of the MIP/PLL/PEDOT−PG indicates that the incorporation of PG into PEDOT, with PLL uniformly wrapping its surface, significantly enhanced conductivity, carrier mobility, stability, and provided a larger surface area for specific recognition sites. Under optimal experimental conditions, the electrochemical response exhibited a linear relationship with a logarithm of DBP concentration within the range of 1 fM to 5 µM, with the detection limit as low as 0.88 fM. The method demonstrated exceptional stability and repeatability and has been successfully applied to quantify DBP in plastic packaging materials.
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ACS Applied Engineering Materials
·
2025
·
IF 3.5
Yihan Zhang,Yiliyasi Baikeli,Zehong Gao,Longyi Chen
Development of polysaccharide biomass for efficient electrocatalytic sensing applications is important for its natural resource utilization and detection application. Elaeagnus angustifolia L. gum is chosen as an electron-rich macromolecule of polysaccharide as the encapsulating agent to surround etched parted ZIF-67(Co) particles. The three electron-rich molecules trithiocyanuric acid, urea, and sodium hypophosphite are selected as the weak acid etching agents, which could etch ZIF-67(Co) microcubes. This fragmentation of ZIF-67(Co) is attributed to the electron-rich molecules disrupting the internal coordination bonds of ZIF-67(Co), thus producing fragmented particles with increased exposed active electrocatalytic spots. Further carbonization of the mixture is processed to obtain the electrocatalyst ZIF-67(Co)-NPS-doped biomass carbon (ZNBC). The ZNBC/GCE sensor shows a significant improvement in electrochemical performance with a linear range of 0.5 to 100 μM, a sensitivity of 10.04 μA μM–1 cm–2, a limit of detection of 0.052 μM, and good selectivity. The ZNBC/GCE sensor was also used in rapid and accurate electrochemical sensing of APAP in urine, lake water, and pharmaceutical capsule preparation samples with good recoveries. This electron-rich macromolecule/molecule as an encapsulating/etching combo strategy provides insight into the usage of Elaeagnus angustifolia L. gum and top-down design of metal–organic frameworks (MOFs) for potential applications in electrocatalyst design.
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BMC PLANT BIOLOGY
·
2025
·Vol. 25
·期 1
·1613
·
IF 4.8
Ghanaim Amira M.,Mahmoud Ghada A.,Mohamed Heba I.,Hanafy Rania S.,Zaki Laila M.,Mahmoud Mohamed,Mogazy Asmaa M.
Background Plant viral infections threaten global food security and cause major crop losses. This study investigates the antiviral effects of biosynthesized Chitosan nanoparticles (ChNPs) with Bacillus subtilis 1211 EMCCN against potato virus Y (PVY) and their influence on tomato growth and yield. Results Scanning electron microscopy showed clusters of crystals on ChNPs, while transmission electron microscopy revealed that the nanoparticles were spherical, ranging from 12 to 198 nm. Energy-dispersive X-ray spectroscopy confirmed the presence of carbon, oxygen, sodium, and phosphorus, and Fourier-transform infrared analysis identified typical chitosan functional groups, including hydroxyl, carbonyl, and amine. Tomato leaves were treated with ChNPs, B. subtilis , or both to evaluate their effectiveness against PVY. The treated plants exhibited a marked reduction in both disease severity and PVY concentration compared to the untreated controls. By 28 days post-inoculation, infectivity decreased to 46.7% in plants treated with Bacillus subtilis, 33.3% with ChNPs, and reached the lowest level of 20% in the combined treatment. The combined priming strategy significantly enhanced plant growth attributes, with increases in shoot length (95.0%), root length (47.0%), leaf area (668.1%), plant height (30.7%), shoot and root fresh weight (370.0% and 162.9%), and shoot and root dry weight (562.7% and 127.7%). Biochemical and physiological analyses revealed substantial increases in total pigments (268.4%), flavonoids (112.2%), phenols (59.4%), α-tocopherol (92.1%), ascorbic acid (30.1%), anthocyanins (71.3%), peroxidase (51.9%), catalase (39.6%), polyphenol oxidase (73.6%), amino acids (119.9%), proline (93.3%), soluble sugars (128.0%), and proteins (614.0%) in shoots. Yield components, including carotenoids and anthocyanins, were also significantly enhanced. Importantly, oxidative stress indicators were markedly reduced, with malondialdehyde and hydrogen peroxide levels decreasing by 76.8% and 72.6%, respectively, in treated plants compared to infected plants. Conclusion These findings suggest that combination between ChNPs and B. subtilis offers an eco-friendly method to enhance tomato yield and effectively manage viral diseases by activating the plants' defense mechanisms.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2014
·
IF 2.694
Lingxiao Chen,Wenhua Ji,Wenjuan Duan,Xiao Wang,Qianshan Gao,Yanling Geng,Luqi Huang
Molecularly imprinted polymers (MIPs) with high selectivity and affinity to glabridin were designed based on the screening results of the library of non-imprinted polymers (NIPs). The NIP library contained 48 polymers that were polymerized with the combinations of different functional monomers, cross-linkers, and porogenic solvents. The distribution coefficient ( k ) values were used to estimate the affinity of NIPs to glabridin. The corresponding MIPs of the best three NIPs were prepared. After evaluating the imprinting effects and selectivity of the three MIPs, the performance of the best MIP as solid phase extraction sorbent was investigated. Glabridin with percent recovery of 83 was obtained from the extract of Glycyrrhiza glabra L. ( G. glabra L.) residues by molecularly imprinted solid phase extraction (MISPE). Thus, this material can be successfully used for the extraction and purification of glabridin from G. glabra L. residues.
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Foods
·
2025
·Vol. 14
·期 16
·2800
·
IF 5.1
Yangzu Gao,Haoyu Li,Qian Wu,Bang Chen,Kangzhen Xu,Cong Li,Yehua Shen
The functional exploration of natural foods, coupled with the increasing prevalence of gastrointestinal motility disorders and the associated therapeutic challenges, has generated significant interest in this field. This study aims to investigate the ameliorative effects of the extract fromPugionium cornutum(L.) Gaertn (EAEPC), a traditional edible vegetable in northwest China’s desert region, on atropine-induced gastroparesis in mice, as well as to elucidate its mechanism in terms of the gut microbiota and major metabolites. The findings indicate that EAEPC effectively reduces the rate of pigment residual in the stomach while shortening the gastrointestinal transit time and alleviating other symptoms associated with atropine-induced gastroparesis. These effects may be mediated through modulation of the expression levels of major intestinal metabolites, such as short-chain fatty acids (SCFAs), bile acids (BAs), and L-tryptophan, alongside remodeling of both the diversity and relative abundance of the gut microbiota. Furthermore, correlation analyses were conducted on significantly altered strains and metabolites to clarify their interactions. Moreover, the chemical constituents of EAEPC were identified by UPLC-Q-TOF-MS/MS, and the key active components responsible for improving gastroparesis were predicted through network pharmacology approaches and validated experimentally. These results provide a foundation for further research into the functions ofPugioniumand offer scientific support for developing natural plant-based strategies aimed at treating gastrointestinal motility disorders.
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MOLECULES
·
2025
·Vol. 30
·期 20
·4070
·
IF 4.6
Ru Zhao,Wenjun Xu,Yuxiang Tang,Jinwen Liu,Xiaoli Li,Liangui Tan,Ailing Ben,Tingli Liu,Lei Yang
The ultrasound-assisted antisolvent precipitation method was used to prepare lignin nanoparticles fromThlaspi arvenseL. rhizomes. The influence of each experimental variable on the average particle size (APS) of the lignin nanoparticles was determined via single-factor experiments. The optimal conditions for the preparation of the lignin nanoparticles were investigated in detail, and the APS of the lignin nanoparticles was 118 ± 3 nm. Compared with those of untreated lignin, the lignin nanoparticles prepared via this method were spherical and evenly distributed, and the structure was not damaged. Ultrasound generated local extreme physical conditions through its cavitation effect to promote nucleation, triggered high-speed turbulence to refine the particle size and improve uniformity, and applied mechanical disturbance to inhibit particle agglomeration, which promoted the preparation of lignin nanoparticles with a small size and good dispersion. A solubility test revealed that the lignin nanoparticles had greater solubility, which was improved 9-fold. The determination of antioxidant capacity revealed that the lignin nanoparticles had high free radical scavenging activity, which provided a broader space for the multifaceted utilization of a kind of grass lignin with the structural characteristics ofT. arvenselignin (p-hydroxyphenyl lignin).
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American Journal of Translational Research
·
2019
·
IF 3.266
Zhongyue Huang,Huijie Gu,Xiaofan Yin,Long Gao,Kunxi Zhang,Yiming Zhang,Jun Xu,Liang Wu,Jingbo Yin,Lei Cui
Microspheres have gained immense popularity in bone tissue engineering because of their unique properties as injectable scaffolds for bone tissue regeneration. Herein, we evaluated the feasibility of using poly (γ-benzyl-L-glutamate) (PBLG) microspheres for bone engineering by examining the attachment, proliferation, and osteogenic differentiation of adipose-derived stem cells (ASCs) in vitro and the use of PBLG microspheres in healing non-union in vivo . Scanning electron microscopy and fluorescent 3, 30-dioctadecyloxacarbocyanine perchlorate-labeling were performed to study the attachment and growth of ASCs to the PBLG microspheres, and a DNA assay was performed to quantify cell proliferation with time. Osteogenic differentiation of ASCs cultured on the PBLG microspheres was assessed by determining alkaline phosphatase expression and extracellular calcium deposition, which was further confirmed by real-time polymerase chain reaction for osteogenesis-related genes. Femoral non-union were created in mouse models and filled with ASCs/PBLG microspheres in vivo . The results showed that ASCs attached, spread, and showed good osteogenic differentiation on the PBLG microspheres in vitro . Moreover, the ASCs/PBLG microspheres could repair the mouse femoral non-union in vivo . Thus, PBLG microspheres have good biocompatibility and cytocompatibility and are potentially useful as an injectable scaffolds for bone tissue engineering.
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Journal of Biobased Materials and Bioenergy
·
2021
·Vol. 15
·期 4
·553-558
·
IF 0.708
Chen Zhifeng,Guo Haifeng,Sui Changling,Gao Zhixi,Wang Tianhong,Luo Yong,Qin Cheng,Guan Xiaoxi
Drought and salt are main environmental factors that affect the growth, development, productivity and distribution of plants of plants. Alfalfa has a strong ability of early and salt resistance. In this work, the varieties Xinjiang Daye was used as material, the effects of drought (simulated
with PEG, polyethylene glycol-6000) and salt stress (with NaCl solution) on the antioxidant capacity of alfalfa seedlings and stress resistance genes was studied, to select alfalfa varieties with strong resistance and study its functional principle of resistance related genes. The results
showed that with the increase of drought stress, the contents of H2O2, O2−, MDA increased by 323, 247 and 235 (15% PEG treatment). The activities of SOD, CAT and APX increased by 18.01, 15.56 and 587% (15% PEG treatment), respectively. The expression
of drought resistance genes increased significantly. With the increase in NaCl stress, the activities of SOD, cat, pod and APX increased by 132.14, 315.60, 102.78 and 27.61%, respectively. The expression of two genes related to salt stress increased significantly. In conclusion, alfalfa leaves
have good survival ability under high stress, and the activities of antioxidant enzymes and the expression of related genes have adaptive changes under drought and salt stress.
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MOLECULAR PLANT PATHOLOGY
·
2021
·Vol. 23
·期 1
·60-77
·
IF 5.663
Changyun Liu,Shaorui Tian,Xing Lv,Yundan Pu,Haoran Peng,Guangjin Fan,Xiaozhou Ma,Lisong Ma,Xianchao Sun
Asparagine synthetase is a key enzyme that catalyses the conversion of amide groups from glutamine or ammonium to aspartate, which leads to the generation of asparagine. However, the role of asparagine synthetase in plant immunity remains largely unknown. Here, we identified a Nicotiana benthamiana asparagine synthetase B (NbAS-B) that associates with tomato mosaic virus coat protein-interacting protein L (IP-L) using the yeast two-hybrid assay and examined its role in tobacco mosaic virus (TMV) resistance. The association of IP-L with NbAS-B was further confirmed by in vivo co-immunoprecipitation, luciferase complementation imaging, and bimolecular fluorescence complementation assays. IP-L and NbAS-B interact in the nucleus and cytosol and IP-L apparently stabilizes NbAS-B, thus enhancing its accumulation. The expressions of IP-L and NbAS-B are continuously induced on TMV-green fluorescent protein (GFP) infection. Co-silencing of IP-L and NbAS-B facilitates TMV-GFP infection. Overexpression of NbAS-B in tobacco reduces TMV-GFP infection by significantly improving the synthesis of asparagine. Furthermore, the external application of asparagine significantly inhibits the infection of TMV-GFP by activating the salicylic acid signalling pathway. These findings hold the potential for the future application of asparagine in the control of TMV.
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